subject:(intrinsically disordered region)

University of Colorado

1. Protter, David Stephen Warren. Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules.

Degree: PhD, Chemistry & Biochemistry, 2017, University of Colorado

URL: https://scholar.colorado.edu/chem_gradetds/242

► Cells assemble large, non-membrane bound granules of protein and RNA, termed Ri- bonucleoprotein granules (RNP granules), often in response to a wide variety of… (more)

Subjects/Keywords: intrinsically disordered region; phase separation; RNP granule; stress granule; Biochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Protter, D. S. W. (2017). Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules. (Doctoral Dissertation). University of Colorado. Retrieved from https://scholar.colorado.edu/chem_gradetds/242

Chicago Manual of Style (16^th Edition):

Protter, David Stephen Warren. “Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules.” 2017. Doctoral Dissertation, University of Colorado. Accessed April 13, 2021. https://scholar.colorado.edu/chem_gradetds/242.

MLA Handbook (7^th Edition):

Protter, David Stephen Warren. “Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules.” 2017. Web. 13 Apr 2021.

Vancouver:

Protter DSW. Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules. [Internet] [Doctoral dissertation]. University of Colorado; 2017. [cited 2021 Apr 13]. Available from: https://scholar.colorado.edu/chem_gradetds/242.

Council of Science Editors:

Protter DSW. Contributions of Intrinsically Disordered Regions of Proteins to the Assembly of Ribonucleoprotein Granules. [Doctoral Dissertation]. University of Colorado; 2017. Available from: https://scholar.colorado.edu/chem_gradetds/242

University of California – San Diego

2. Fang, Mark Yang. Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD.

Degree: Biomedical Sciences, 2019, University of California – San Diego

URL: http://www.escholarship.org/uc/item/1c0562nh

► Stress granules (SG) form during cellular stress and have been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD). Strategies to… (more)

Subjects/Keywords: Neurosciences; Cellular biology; ALS; intrinsically disordered region; RNA binding protein; screening; stem cell disease model; stress granule

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fang, M. Y. (2019). Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/1c0562nh

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fang, Mark Yang. “Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD.” 2019. Thesis, University of California – San Diego. Accessed April 13, 2021. http://www.escholarship.org/uc/item/1c0562nh.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fang, Mark Yang. “Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD.” 2019. Web. 13 Apr 2021.

Vancouver:

Fang MY. Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD. [Internet] [Thesis]. University of California – San Diego; 2019. [cited 2021 Apr 13]. Available from: http://www.escholarship.org/uc/item/1c0562nh.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fang MY. Modulation of RNA-dependent Interactions in Stress Granules Prevents Persistent TPD-43 accumulation in ALS/FTD. [Thesis]. University of California – San Diego; 2019. Available from: http://www.escholarship.org/uc/item/1c0562nh

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

3. Mouapi, Kelly Njine. Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity.

Degree: PhD, 2017, University of Louisville

URL: 10.18297/etd/2770 ; https://ir.library.louisville.edu/etd/2770

► Fibrinogen is the most abundant protein involved in blood coagulation and has been associated with many pathological implications in cardiovascular disease. At the final… (more)

▼ Fibrinogen is the most abundant protein involved in blood coagulation and has been associated with many pathological implications in cardiovascular disease. At the final stages of blood clot formation, the transglutaminase Factor XIIIa introduces γ-glutamyl-ε-lysinyl covalent bonds between reactive glutamines and lysines in fibrin, which results in a tighter clot network that is resistant to fibrinolysis. Factor XIIIa crosslinks specific reactive glutamines on fibrinogen, selecting more reactive glutamines in the αC region of fibrinogen than any other chain. Although crosslinking pairs in the αC region have been identified, little is known about the extent of crosslinking and the role played by each reactive glutamine. For the first time, we have ranked three reactive glutamines crosslinked under physiological conditions. A combination of MALDI-TOF mass spectrometry, LC-MS, and 2D ¹⁵N-¹H HSQC NMR studies was used on Fibrinogen aC(233-425) to understand Factor XIIIa’s substrate specificity. Factor XIIIa’s specificity for each reactive glutamine in aC(233-425) was monitored by the enzyme's ability to cross-link a lysine mimic, glycine ethyl ester (GEE), to the reactive glutamines (Q237, Q328, Q366). We showed that Factor XIIIa crosslinks these three glutamines to GEE in the order Q237 >> Q366 ≈ Q328. Fibrinogen aC(233-425) variants were generated in which each reactive glutamine (Q) was replaced with an inactive asparagine (N). We demonstrated that Factor XIIIa still crosslinks each reactive glutamine independently and is more selective in the absence of the most reactive glutamine Q237. To examine the role of the putative Factor XIII binding site on αC(233-425), we performed experiments in which an important amino acid E396 was replaced with an alanine (Fbg αCE396A). Our results showed that both plasma Factor XIII A₂B₂ and recombinant Factor XIII A₂ can crosslink all three reactive glutamines in Fbg αCE396A to a similar extent as with the WT. For structural characterization, aC(233-425) was expressed in ¹⁵N or ¹⁵N/¹³C-enriched media. 2D HSQC NMR experiments confirmed that the ¹⁵N-labeled αC(233-425) is mostly intrinsically disordered. Residues surrounding Q366 and Factor XIII's binding site αC(389-402) showed a higher propensity for order. 2D NMR experiments suggest the possibility of pre-structured motifs (PreSMOS) within Fibrinogen aC(233-425). Advisors/Committee Members: Maurer, Muriel, Merchant, Michael, Merchant, Michael, Mueller, Eugene, Wittebort, Ricard.

Subjects/Keywords: fibrinogen; αC region; Factor XIII; intrinsically disordered proteins; mass spectrometry; NMR spectroscopy; Biochemistry; Biochemistry, Biophysics, and Structural Biology; Laboratory and Basic Science Research; Life Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mouapi, K. N. (2017). Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/2770 ; https://ir.library.louisville.edu/etd/2770

Chicago Manual of Style (16^th Edition):

Mouapi, Kelly Njine. “Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity.” 2017. Doctoral Dissertation, University of Louisville. Accessed April 13, 2021. 10.18297/etd/2770 ; https://ir.library.louisville.edu/etd/2770.

MLA Handbook (7^th Edition):

Mouapi, Kelly Njine. “Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity.” 2017. Web. 13 Apr 2021.

Vancouver:

Mouapi KN. Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity. [Internet] [Doctoral dissertation]. University of Louisville; 2017. [cited 2021 Apr 13]. Available from: 10.18297/etd/2770 ; https://ir.library.louisville.edu/etd/2770.

Council of Science Editors:

Mouapi KN. Characterizing reactive glutamines in fibrinogen and elucidating factor XIII substrate specificity. [Doctoral Dissertation]. University of Louisville; 2017. Available from: 10.18297/etd/2770 ; https://ir.library.louisville.edu/etd/2770

Virginia Commonwealth University

4. Desai, Megha. Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex.

Degree: PhD, Human Genetics, 2014, Virginia Commonwealth University

URL: https://doi.org/10.25772/FESX-3E41 ; https://scholarscompass.vcu.edu/etd/3617

► The MBD2-NuRD co-repressor complex is an epigenetic regulator of the developmental silencing of embryonic and fetal β-type globin genes in adult erythroid cells as… (more)

Subjects/Keywords: MBD2; globin; epigenetics; tumor suppressor PRSS8; intrinsically disordered region; histone deacetylase; Biochemistry, Biophysics, and Structural Biology; Genetics; Genomics; Molecular Biology; Molecular Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Desai, M. (2014). Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex. (Doctoral Dissertation). Virginia Commonwealth University. Retrieved from https://doi.org/10.25772/FESX-3E41 ; https://scholarscompass.vcu.edu/etd/3617

Chicago Manual of Style (16^th Edition):

Desai, Megha. “Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex.” 2014. Doctoral Dissertation, Virginia Commonwealth University. Accessed April 13, 2021. https://doi.org/10.25772/FESX-3E41 ; https://scholarscompass.vcu.edu/etd/3617.

MLA Handbook (7^th Edition):

Desai, Megha. “Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex.” 2014. Web. 13 Apr 2021.

Vancouver:

Desai M. Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex. [Internet] [Doctoral dissertation]. Virginia Commonwealth University; 2014. [cited 2021 Apr 13]. Available from: https://doi.org/10.25772/FESX-3E41 ; https://scholarscompass.vcu.edu/etd/3617.

Council of Science Editors:

Desai M. Structural and Functional Characterization of the MBD2-NuRD Co-Repressor Complex. [Doctoral Dissertation]. Virginia Commonwealth University; 2014. Available from: https://doi.org/10.25772/FESX-3E41 ; https://scholarscompass.vcu.edu/etd/3617

Universitat Pompeu Fabra

5. Agostini, Federico, 1985-. Predictions of RNA-binding ability and aggregation propensity of proteins.

Degree: Departament de Ciències Experimentals i de la Salut, 2014, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/318159

► Las proteínas de unión de ARN son responsables de controlar el destino de una multitud de transcriptos codificantes y no codificantes. De hecho, la formación… (more)

Subjects/Keywords: Intrinsically disordered region (IDR); Long non-coding RNA (lncRNA); Low-complexity sequence (LCS); Protein aggregate; Ribonucleoprotein complex (RNP complex); RNA granule; RNA recognition element (RRE); RNA-binding protein (RBP); Stress granules; Región intrínsecamente desordenada; ARN largo no codificante; Secuencia de baja complejidad; Agregado proteico; Complejo de ribonucleoproteína; Gránulo de ARN; Elemento de reconocimiento de ARN; Proteína de unión al ARN; Gránulos de estrés; 577

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Agostini, Federico, 1. (2014). Predictions of RNA-binding ability and aggregation propensity of proteins. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/318159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Agostini, Federico, 1985-. “Predictions of RNA-binding ability and aggregation propensity of proteins.” 2014. Thesis, Universitat Pompeu Fabra. Accessed April 13, 2021. http://hdl.handle.net/10803/318159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Agostini, Federico, 1985-. “Predictions of RNA-binding ability and aggregation propensity of proteins.” 2014. Web. 13 Apr 2021.

Vancouver:

Agostini, Federico 1. Predictions of RNA-binding ability and aggregation propensity of proteins. [Internet] [Thesis]. Universitat Pompeu Fabra; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10803/318159.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Agostini, Federico 1. Predictions of RNA-binding ability and aggregation propensity of proteins. [Thesis]. Universitat Pompeu Fabra; 2014. Available from: http://hdl.handle.net/10803/318159

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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