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University of New Mexico

1. Nolan, Journey R. Molecular characterization of the freshwater snail Physella acuta.

Degree: UNM Biology Department, 2013, University of New Mexico

Current insights into general animal biology, including immune function, are derived mostly from a few model organisms, a necessity imposed by effort required for in-depth studies. Modern next-generation sequencing (NGS) enables genomics-level investigations to test whether specific aspects of biology are general or unique across animal phylogeny. Meaningful comparisons require thorough identification of phylogenetically-relevant species. To provide a touchstone for Biomphalaria glabrata, a freshwater snail that is a major study model for invertebrate immunity, physid snails (Physidae; Hygrophila; Panpulmonata; Gastropoda; Mollusca) will be developed as a model for comparative immunogenomics. Chapter 1 introduces current research capabilities and details the rationale behind developing a physid as a comparative model for B. glabrata. Chapter 2, submitted as a manuscript to the Journal of Molluscan Studies, describes the molecular characterization that was performed for species identification and confirms taxonomic placement of physid snails relative to B. glabrata. Comparative mitogenomics revealed high levels of intraspecific sequence divergence between the entire mitochondrial genomes of the two P. acuta isolates, identified a unique gene order, and compared substitutions rates of gene sequences from the mitochondrial genomes of other gastropods. Additional materials are provided in the Appendices; Appendix A provides supplemental information for Chapter 2 and Appendix B provides a report of preliminary results from NGS-based gene discovery for initial characterization of the immune response of P. acuta to bacterial exposure toward comparative immunogenomics. Results from this thesis support continuing efforts to develop P. acuta as a comparative immunogenomic model to B. glabrata. Advisors/Committee Members: Adema, Dr. Coenraad M., Stricker, Dr. Stephen, Takacs-Vesbach, Dr. Cristina.

Subjects/Keywords: gastropod; Physella acuta; Physa acuta; mitochondria; mitogenomics; transcriptomics; 454; next generational sequencing; Biomphalaria glabrata; gene rearrangements; dN/dS; Ka/Ks; intraspecific sequence differences; invertebrate immunology; lophotrochozoa; Mollusca; FREP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Nolan, J. R. (2013). Molecular characterization of the freshwater snail Physella acuta. (Masters Thesis). University of New Mexico. Retrieved from https://digitalrepository.unm.edu/biol_etds/87

Chicago Manual of Style (16th Edition):

Nolan, Journey R. “Molecular characterization of the freshwater snail Physella acuta.” 2013. Masters Thesis, University of New Mexico. Accessed April 16, 2021. https://digitalrepository.unm.edu/biol_etds/87.

MLA Handbook (7th Edition):

Nolan, Journey R. “Molecular characterization of the freshwater snail Physella acuta.” 2013. Web. 16 Apr 2021.

Vancouver:

Nolan JR. Molecular characterization of the freshwater snail Physella acuta. [Internet] [Masters thesis]. University of New Mexico; 2013. [cited 2021 Apr 16]. Available from: https://digitalrepository.unm.edu/biol_etds/87.

Council of Science Editors:

Nolan JR. Molecular characterization of the freshwater snail Physella acuta. [Masters Thesis]. University of New Mexico; 2013. Available from: https://digitalrepository.unm.edu/biol_etds/87

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