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You searched for subject:(intracellular storage). Showing records 1 – 3 of 3 total matches.

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1. Phan, Nolwenn Kathryn. Lipid body function as non-classical calcium stores in immunocytes.

Degree: 2015, University of Hawaii – Manoa

M.S. University of Hawaii at Manoa 2014.

Lipid bodies, found in most eukaryotic cells, are intracellular lipid storage organelles. The role of lipid bodies has been most studied in adipocytes and hepatocytes due to their role in energy storage. Comparatively, little is known of the role they play in immunocytes. Both in adipocytes/hepatocytes and in cells of the immune system, lipid body numbers are dynamically regulated and can accumulate to pathophysiological levels (steatosis). In both locales this steatotic state can be induced by nutrient overload and metabolic stress, and in the latter also under certain conditions of infection. The over-arching goals of this project are (1) to study the composition (lipid and protein) and functional contributions made by lipid bodies in immunocytes, and (2) to assess the impact of altered lipid body numbers upon cellular function. The work presented in this thesis describes three areas of progress towards these goals. First, we developed a microaspiration method for isolating highly purified lipid bodies, allowing for their ex vitro manipulation and study of lipid/protein content using microscopy. This technique was validated using fluorescence microscopy of the neutral lipid dye Oil Red O in microaspirated lipid bodies. Second, we assessed the impact of the accumulation of lipid bodies (steatosis) on transcytoplasmic calcium signaling, a major activation pathway in the model immune cell system studied here. Third, we tested a new hypothesis arising from our work on transcytoplasmic calcium signaling. The apparent ability of lipid bodies to act as long term loci for calcium accumulation, coupled with recent studies showing that lipid bodies may contain mitochondria and endoplasmic reticulum, led us to hypothesize their potential role as bona fide calcium stores. Our data revealed that the lipid body population within mast cells is not homogenous. However, some LB exhibit the ability to sequester calcium and to release it in the manner of a bona fide calcium store. These observations represent a potentially novel role for lipid bodies and indicate the possibility of their contribution to calcium dynamics in immune cells under both physiological and pathophysiological conditions.

Subjects/Keywords: intracellular lipid storage organelles

…organisms, LB are most appreciated for their role as intracellular lipid storage units and have… …a role in the regulate lipid storage metabolism (26-29). Figure 1.1… …plays a central role in cell signaling. Calcium is a ubiquitous intracellular signaling ion… …concentrations of calcium are in the millimolar (mM) range while intracellular calcium is in… …intracellular calcium stores could provide more insight into the calcium signaling dynamics of all… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Phan, N. K. (2015). Lipid body function as non-classical calcium stores in immunocytes. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Phan, Nolwenn Kathryn. “Lipid body function as non-classical calcium stores in immunocytes.” 2015. Thesis, University of Hawaii – Manoa. Accessed June 04, 2020. http://hdl.handle.net/10125/101219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Phan, Nolwenn Kathryn. “Lipid body function as non-classical calcium stores in immunocytes.” 2015. Web. 04 Jun 2020.

Vancouver:

Phan NK. Lipid body function as non-classical calcium stores in immunocytes. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2020 Jun 04]. Available from: http://hdl.handle.net/10125/101219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Phan NK. Lipid body function as non-classical calcium stores in immunocytes. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/101219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Le Saux, Sarah. Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors.

Degree: Docteur es, Biologie Santé, 2019, Montpellier

Les vésicules extracellulaires (VE) sont des nano-vésicules produites par les cellules : elles jouent un rôle clé dans la communication intercellulaire en délivrant des molécules, acides nucléiques et protéines. Grâce à ces propriétés naturelles, les VE ont émergé en tant que nouveaux vecteurs. La délivrance intracellulaire de protéines est particulièrement difficile, et les systèmes existants présentent des défauts (biocompatibilité, sûreté) : les VE pourraient être des candidats singulièrement intéressants. Mon objectif de thèse était d’évaluer le potentiel des VE pour la délivrance intracellulaire de fragment d’anticorps, en utilisant des processus de chargement physico-chimiques sur des VE après production. Le premier objectif était de produire, caractériser et conserver les VE. Les VE ont été isolés à partir de cellules souches mésenchymateuses murines par ultracentrifugation. Ils ont été caractérisés de façon exhaustive : les VE étaient sphériques, de 94±10 nm de diamètre, possédaient des protéines (TSG101, CD81) et des lipides (cholestérol, sphingomyélines, céramides) spécifiques de VE de petite taille. Les VE étaient davantage internalisés que les liposomes (standards, HSPC/Cholestérol) in vitro et ils suivent des voies d’endocytose différentes. Congeler les VE dans du trehalose (25 mM) avec inhibiteurs de protéase assurait une conservation optimale. Le deuxième objectif était d’utiliser des processus physico-chimiques pour charger dans les VE un fragment d’anticorps single-chain variable (scFv) avec un tag GFP et ciblant la tubuline humaine, et de parvenir à délivrer un scFv fonctionnel en intracellulaire. Différentes méthodes ont été évaluées : extrusion, sonication avec une sonde, bain à ultrasons, lyophilisation, cycles de congélation/décongélation, et des combinaisons de ces méthodes. Nous avons identifié des protocoles de chargement résultant en des pertes de VE et scFv inférieures à 20%. Pour certains procédés combinatoires, un léger marquage tubuline a été observé in vitro, ce qui implique que les complexes VE-scFv ont été internalisés, ont échappé à la dégradation endosomale et ont atteint la tubuline dans le cytoplasme. Ces résultats devront être confirmés, mais nous concluons que les VE semblent prometteurs en tant que système pour la délivrance intracellulaire d’anticorps fonctionnels, ce qui n’a jamais été décrit auparavant.

Extracellular vesicles (EVs) are nano-vesicles produced by cells: they play a key role in intercellular communication by delivering molecules, nucleic acids or proteins. Because of these natural features, EVs have emerged as novel vectors. Intracellular protein delivery is particularly challenging, and existing systems have shortcomings (biocompatibility, safety): EVs could be an especially interesting candidate. My objective for this PhD was to evaluate the potential of EVs for antibody fragment intracellular delivery, using physico-chemical loading processes on EVs once isolated. The first goal was to isolate, characterise and store nanosized EVs. EVs were isolated from…

Advisors/Committee Members: Legrand, Philippe (thesis director), Morille, Marie (thesis director).

Subjects/Keywords: Vésicules extracellulaires; Délivrance intracellulaire; Modifications post-Production; Conservation; Voie d'endocytose; Anticorps; Extracellular vesicles; Intracellular delivery; Post-Production modifications; Storage; Endocytic pathway; Antibody

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Le Saux, S. (2019). Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2019MONTT055

Chicago Manual of Style (16th Edition):

Le Saux, Sarah. “Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors.” 2019. Doctoral Dissertation, Montpellier. Accessed June 04, 2020. http://www.theses.fr/2019MONTT055.

MLA Handbook (7th Edition):

Le Saux, Sarah. “Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors.” 2019. Web. 04 Jun 2020.

Vancouver:

Le Saux S. Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors. [Internet] [Doctoral dissertation]. Montpellier; 2019. [cited 2020 Jun 04]. Available from: http://www.theses.fr/2019MONTT055.

Council of Science Editors:

Le Saux S. Exploration du potentiel des vésicules extracellulaires en tant que vecteurs de protéines thérapeutiques : Exploring the potential of extracellular vesicles as therapeutic protein delivery vectors. [Doctoral Dissertation]. Montpellier; 2019. Available from: http://www.theses.fr/2019MONTT055


Virginia Tech

3. Erdal, Zeynep Kisoglu. An Investigation of the Biochemistry of Biological Phosphorus Removal.

Degree: PhD, Civil Engineering, 2002, Virginia Tech

Although enhanced biological phosphorus removal (EBPR) and complete biological nutrient removal (BNR) systems can be operated successfully by experienced operators, the accuracy of design and strength of the scientific background need to be reinforced to enable accurate modeling and economically optimal design. One way to accomplish this would be through a better understanding of the biochemical mechanisms and microbial population dynamics that determine the reliability and efficiency of EBPR, and the utilization of this information to improve the design and operation of BNR plants. Such knowledge will also contribute to better structure of modeling tools that are used for design and educational purposes. The current body of knowledge is limited to observational studies that lack detailed biochemical explanations backed with a series of well planned experiments, and this has introduced uncertainties and inaccuracies into the biochemical and design models. Therefore, this study mainly covers a biochemical survey of the underlying metabolisms of active populations in BNR sludges. BNR biomass with biological phosphorus removal (BPR) capability was cultivated in continuous flow reactor (CFR) systems, configured as either University of Cape Town (UCT) and anoxic/oxic (A/O) systems. Following an acclimation period at 20°C, low temperature stress (5°C) was imposed on one UCT system for investigation of the response of the microbial consortium responsible from EBPR activity under cold temperature. Once a stable population with EBPR capabilities is established in each system, activities of ten enzymes that are hypothesized to be taking part in the EBPR metabolism were measured. These enzymes were selected among those that take part in major known pathways of bacterial energy and growth metabolism. Also, 13C-NMR was used as a tool to monitor the flux of labeled carbon in and out of pools of cellular storage; i.e. glycogen and polyhydroxyalkanoates (PHA). Combining the gathered information, accurate mass balances of carbons and reducing equivalents were calculated, eventually leading to determination of the biochemical pathways utilized by the EBPR consortium. Additionally, anaerobic stabilization of COD, a long debated but empirically established phenomenon, was addressed during the study. Considering the pathways proposed to be operative under different conditions imposed on the EBPR systems, a biochemical explanation for the occurrence of COD stabilization in wastewater treatment systems that incorporate anaerobic zones was proposed. Accordingly, depending on the pathways actively used by a microbial consortium, electrons stored in NADH and FADH2 can either be transferred to the terminal electron acceptor, oxygen, or they can be incorporated into storage polymers such as glycogen for future use. Such differences in metabolism reflect in the quantity of the oxygen consumed in the aerobic reactors. Thus, the correct incorporation of anaerobic stabilization of COD into process design would reduce design aeration… Advisors/Committee Members: Randall, Clifford W. (committeechair), Wightman, James P. (committee member), Gallagher, Daniel L. (committee member), Boardman, Gregory D. (committee member), Gregory, Eugene M. (committee member).

Subjects/Keywords: energy metabolism; enzyme activity; polyhydroxyalkanoates; glycogen; biochemical model; intracellular storage; anaerobic stabilization; activated sludge; competition; biological phosphorus removal

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Erdal, Z. K. (2002). An Investigation of the Biochemistry of Biological Phosphorus Removal. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/26383

Chicago Manual of Style (16th Edition):

Erdal, Zeynep Kisoglu. “An Investigation of the Biochemistry of Biological Phosphorus Removal.” 2002. Doctoral Dissertation, Virginia Tech. Accessed June 04, 2020. http://hdl.handle.net/10919/26383.

MLA Handbook (7th Edition):

Erdal, Zeynep Kisoglu. “An Investigation of the Biochemistry of Biological Phosphorus Removal.” 2002. Web. 04 Jun 2020.

Vancouver:

Erdal ZK. An Investigation of the Biochemistry of Biological Phosphorus Removal. [Internet] [Doctoral dissertation]. Virginia Tech; 2002. [cited 2020 Jun 04]. Available from: http://hdl.handle.net/10919/26383.

Council of Science Editors:

Erdal ZK. An Investigation of the Biochemistry of Biological Phosphorus Removal. [Doctoral Dissertation]. Virginia Tech; 2002. Available from: http://hdl.handle.net/10919/26383

.