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University of Hawaii – Manoa
1.
Greineisen, William Ernest.
Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells.
Degree: 2016, University of Hawaii – Manoa
URL: http://hdl.handle.net/10125/101908 
► Ph.D. University of Hawaii at Manoa 2013.
This thesis presents evidence that mast cells (MC) chronically exposed to insulin respond by developing steatotic levels of…
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▼ Ph.D. University of Hawaii at Manoa 2013.
This thesis presents evidence that mast cells (MC) chronically exposed to insulin respond by developing steatotic levels of cytosolic lipid bodies, suggesting that immunocytes, like hepatocytes and myocytes, are sites of lipid sequestration in response to dysregulated insulin levels. This ectopic lipid accumulation influences mast cell functionality, biasing mast cell phenotype towards the production of bioactive lipid mediators (LTC4) and away from the release of histamine and other secretory granule components.
In the current study we present an analysis of the whole cell and lipid body lipidome in control, and insulin-exposed mast cells. Our data show a significant upregulation in lipid-associated pro-inflammatory precursor molecules in response to chronic insulin exposure. We also show that the lipid body population in these cells are heterogeneous to a previously unsuspected degree.
Moreover, due to the intimate relationship between the endoplasmic reticulum (ER) and lipid body production, we tested the hypothesis that the ER may be altered in response to chronic insulin exposure. Indeed, our data show that (in a manner analogous to observations in hepatocytes from obese models) the ER is reprogrammed towards a lipogenic phenotype, is morphologically distended, is compromised as a calcium store and exhibits certain indicators of a unfolded protein response (UPR)/ER stress response in response to chronic insulin.
Taken together, these data show that chronic insulin exposure in a model mast cell system drives lipidomic remodeling in a manner that alters lipid body formation and mast cell proinflammatory function.
Subjects/Keywords: insulin
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APA (6th Edition):
Greineisen, W. E. (2016). Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/101908
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Greineisen, William Ernest. “Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells.” 2016. Thesis, University of Hawaii – Manoa. Accessed February 27, 2021.
http://hdl.handle.net/10125/101908.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Greineisen, William Ernest. “Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells.” 2016. Web. 27 Feb 2021.
Vancouver:
Greineisen WE. Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10125/101908.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Greineisen WE. Chronic exposure to an insulin-containing lipogenic stimulus results in ectopic cytoplasmic lipid accumulation and altered pro-inflammatory function in mast cells. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/101908
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of New South Wales
2.
Tang, Alice.
Insulin-Sensitive Obesity: Prospective and Interventional Studies.
Degree: Clinical School - St Vincent's Hospital, 2017, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/58936
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47978/SOURCE02?view=true
► BACKGROUND: Individuals with metabolically healthy/insulin-sensitive obesity (MHO/Obsen) have been shown to have a comparable metabolic profile to lean individuals. However, longitudinal change in metabolic parameters…
(more)
▼ BACKGROUND: Individuals with metabolically healthy/
insulin-sensitive obesity (MHO/Obsen) have been shown to have a comparable metabolic profile to lean individuals. However, longitudinal change in metabolic parameters in those with MHO/Obsen has not been comprehensively delineated with the gold-standard measure of
insulin-sensitivity, the hyperinsulinaemic-euglycaemic clamp. The response of MHO/ Obsen cohorts to weight loss is also unclear. This study investigates the metabolic changes in the Obsen phenotype over time and in response to weight loss. METHODS: A cohort of 124 individuals was carefully phenotyped 6 years ago and categorised into lean, overweight/obese
insulin-sensitive (Obsen) or resistant (Obres, using gender based median
insulin-sensitivity cut-offs) and type 2 diabetes mellitus (T2DM). Sixty-nine participants were followed up in this study. A subcohort (n = 13) also underwent a 16-week caloric restriction intervention. A comprehensive assessment of metabolic health, including
insulin sensitivity (using hyperinsulinaemic-euglcyaemic clamp), body fat composition, serum/plasma metabolic markers, energy metabolism and lifestyle parameters, was performed.RESULTS: There was an increase in total body fat, visceral adipose tissue mass, blood pressure and adipose tissue
insulin resistance and a decrease in resting metabolic rate in the whole cohort over 6 years.
Insulin sensitivity appeared to decrease over time in Obsen compared to the other groups. Physical activity increased and sugar intake decreased in Obres and T2DM. Saturated fat and trans-fat intake increased in the Obsen group.
Insulin sensitivity in the 6 years prior to weight loss was inversely correlated to the change in
insulin sensitivity induced by weight loss.
Insulin sensitivity immediately prior to weight loss was inversely correlated to the change in resting metabolic rate induced by weight loss.CONCLUSION: Obsen is not an enduring phenotype over 6 years. Hence, clinical intervention should target all individuals with overweight/obesity, irrespective of apparent metabolic health. However, further investigation into the role that
insulin sensitivity plays in the response to weight loss is required to ensure optimum management in Obsen individuals.
Advisors/Committee Members: Greenfield, Jerry, Garvan Institute of Medical Research, Faculty of Medicine, UNSW, Samocha-Bonet, Dorit, Garvan Institute of Medical Research, Faculty of Medicine, UNSW.
Subjects/Keywords: Insulin resistance; Obesity; Insulin sensitivity
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APA (6th Edition):
Tang, A. (2017). Insulin-Sensitive Obesity: Prospective and Interventional Studies. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/58936 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47978/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Tang, Alice. “Insulin-Sensitive Obesity: Prospective and Interventional Studies.” 2017. Doctoral Dissertation, University of New South Wales. Accessed February 27, 2021.
http://handle.unsw.edu.au/1959.4/58936 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47978/SOURCE02?view=true.
MLA Handbook (7th Edition):
Tang, Alice. “Insulin-Sensitive Obesity: Prospective and Interventional Studies.” 2017. Web. 27 Feb 2021.
Vancouver:
Tang A. Insulin-Sensitive Obesity: Prospective and Interventional Studies. [Internet] [Doctoral dissertation]. University of New South Wales; 2017. [cited 2021 Feb 27].
Available from: http://handle.unsw.edu.au/1959.4/58936 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47978/SOURCE02?view=true.
Council of Science Editors:
Tang A. Insulin-Sensitive Obesity: Prospective and Interventional Studies. [Doctoral Dissertation]. University of New South Wales; 2017. Available from: http://handle.unsw.edu.au/1959.4/58936 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:47978/SOURCE02?view=true
University of Utah
3.
Tidwell, Timothy James.
Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet.
Degree: Honors BS;, Biology;, 2009, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1658/rec/651
► INTRODUCTION: People with diabetes are at high risk for cardiovascular disease; which is the major cause of death in diabetics. Current research suggests that excessive…
(more)
▼ INTRODUCTION: People with diabetes are at high risk for cardiovascular disease; which is the major cause of death in diabetics. Current research suggests that excessive mitochondrial uncoupling is a potential mechanism for cardiac dysfunction in diabetic patients. Uncoupling protein 3 (UCP3) is an inner mitochondrial membrane protein that reduces the amount of reactive oxygen species (ROS) produced in the mitochondrial matrix by uncoupling the mitochondria. Mice lacking UCP3 (UCP3KO) have been shown to accumulate ROS and diacylglycerol in cardiac muscle. These two molecules have been shown to induce insulin resistance in skeletal muscle. The goal of my project was to determine if the absence of UCP3 in the heart will exacerbate diet-induced insulin resistance in the murine heart. MATERIALS AND METHODS: Whole heart homogenates were extracted from four groups of mice: (1) wild type (WT) fed normal chow (NC); (2) WT fed a high fat diet for 10 weeks (HF); (3) UCP3KO NC and (4) UCP3KO HF. Insulin signaling was examined using western-blotting to assess the phosphorylation status of key components of the insulin signaling pathway under non insulin and insulin-stimulated conditions in each group. Values form each group were analyzed using an ANOVA followed by a t test to determine any statistical significant differences between the groups. RESULTS: Phosphorylation of protein kinase B (Akt) (at serine 473 or threonine 308) and the downstream target glycogen synthase kinase 3 p at serine 9 was the same between all the groups. CONCLUSIONS: two major conclusions arise from this work; ( 1 ) 1 0 weeks of high fat diet does not affect insulin signaling in the heart and (2) despite increased ROS and DAG levels in the hearts of UCP3KO mice, insulin sensitivity was maintained. We speculate that UCP3KO mice have developed compensatory mechanisms that prevented the development of insulin resistance despite accumulation of compounds known to induce insulin resistance. These mechanisms remain to be determined in future studies.
Subjects/Keywords: Insulin resistance
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APA (6th Edition):
Tidwell, T. J. (2009). Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1658/rec/651
Chicago Manual of Style (16th Edition):
Tidwell, Timothy James. “Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet.” 2009. Masters Thesis, University of Utah. Accessed February 27, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1658/rec/651.
MLA Handbook (7th Edition):
Tidwell, Timothy James. “Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet.” 2009. Web. 27 Feb 2021.
Vancouver:
Tidwell TJ. Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet. [Internet] [Masters thesis]. University of Utah; 2009. [cited 2021 Feb 27].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1658/rec/651.
Council of Science Editors:
Tidwell TJ. Insulin signaling in the hearts of uncoupling protein three knockout mice on a high fat diet. [Masters Thesis]. University of Utah; 2009. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd2/id/1658/rec/651
Oregon State University
4.
Papaioannou, Stamatios Evangelos.
Studies of the functional tyrosyl residues of insulin.
Degree: PhD, Chemistry, 1966, Oregon State University
URL: http://hdl.handle.net/1957/47023
► Acetylimidazole was used to acetylate the tyrosyl residues of insulin. The kinetics of acetylation were studied by termination of the reaction at several time intervals,…
(more)
▼ Acetylimidazole was used to acetylate the tyrosyl residues
of
insulin. The kinetics of acetylation were studied by termination of
the reaction at several time intervals, isolation of acetylated
insulin
and determination of 0-acetyltyrosyl groups by deacetylation with
hydroxylamine. The deacetylation was also studied kinetically by
means of the absorbancy change during the reaction. The degree of
availability of the tyrosyl groups in native
insulin, and of the
0-acetyltyrosyl groups in the acetylated
insulin was studied by the
above kinetic studies. Two tyrosyl residues were acetylated at a
rate sufficiently more fast than the remaining two so that a diacetyl
derivative could be isolated. This preparation was fully active. The
completely acetylated
insulin was only thirty percent as active as
native
insulin in the mouse convulsion test. Native and acetylated
insulin were inactivated by seventy percent on treatment with hydroxylamine. These and other findings lead to the conclusion that
one or both of the slowly acetylated tyrosyl residues of
insulin are
involved in the mechanism of action of the hormone.
In order to determine the location of the two acetylated
tyrosyl residues, the diacetyl derivative of
insulin was oxidized and
the A and B chains isolated were assayed for acetyl groups. The
diacetyl derivative was also digested separately with trypsin and
chymotrypsin, and each core was also assayed for acetyl groups.
From the results of these assays it was concluded that the diacetyl
derivative of
insulin was acetylated to the A19 and B16 tyrosyl
residues, which are characterized as reactive, while the other two
tyrosyl residues, A14 and B26 are non-reactive or bound. The fact
that both tyrosyl B26, and asparaginyl A21 are bound and essential
for hormone activity suggests a specific tyrosyl B26-carboxylate A21
interaction essential for maintaining the native conformation and the
activity of
insulin. The usefulness of the kinetic and structural
studies employed here for evaluation of the degree of exposure of
tyrosyl residues, selective blocking of tyrosyl groups, and determination
of the location of reactive and non-reactive tyrosyl
residues of
insulin, for the purpose of structure-and-function studies
on other problems, is discussed.
Advisors/Committee Members: Becker, Robert R. (advisor).
Subjects/Keywords: Insulin
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APA (6th Edition):
Papaioannou, S. E. (1966). Studies of the functional tyrosyl residues of insulin. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/47023
Chicago Manual of Style (16th Edition):
Papaioannou, Stamatios Evangelos. “Studies of the functional tyrosyl residues of insulin.” 1966. Doctoral Dissertation, Oregon State University. Accessed February 27, 2021.
http://hdl.handle.net/1957/47023.
MLA Handbook (7th Edition):
Papaioannou, Stamatios Evangelos. “Studies of the functional tyrosyl residues of insulin.” 1966. Web. 27 Feb 2021.
Vancouver:
Papaioannou SE. Studies of the functional tyrosyl residues of insulin. [Internet] [Doctoral dissertation]. Oregon State University; 1966. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1957/47023.
Council of Science Editors:
Papaioannou SE. Studies of the functional tyrosyl residues of insulin. [Doctoral Dissertation]. Oregon State University; 1966. Available from: http://hdl.handle.net/1957/47023
Oregon State University
5.
Wu, Lily Henson Liu.
Preparation and properties of polyglycyl insulin.
Degree: MS, Chemistry, 1966, Oregon State University
URL: http://hdl.handle.net/1957/47267
► In order to understand more about insulin behavior in solution, Zn-free insulin was modified by adding polyglycyl chains to the molecule and then observing the…
(more)
▼ In order to understand more about
insulin behavior in solution,
Zn-free
insulin was modified by adding polyglycyl chains to the
molecule and then observing the effect of the peptide on the behavior
of
insulin under various conditions.
NCA-glycine (N-Carboxyglycine anhydride) of high purity was
reacted with Zn-free
insulin in aqueous solution to form polyglycyl
insulin. It was found by dinitrophenylation, formaldehyde titration
and amino acid analysis that two N-terminal amino acids and one
lysine were acylated. The length of the added glycine peptide varied
with the modification conditions.
Biological tests indicated that the polyglycyl
insulin retained
its biological activity, except for highly substituted derivatives. The
derivatives were resistant to trypsin digestion.
Polyglycyl
insulin was more soluble than native
insulin. The
solubility varied with pH in a manner similar to native
insulin, but
was about three times more soluble between pH 4 and pH 6. Thin film dialysis experiments indicated that the polyglycyl
insulin was heterogeneous with respect to the length of attached
glycine peptides.
Insulin forms aggregates under various conditions. In the
present study the gel filtration technique was used to analyze the
aggregation state of modified insulins. The polyglycyl-
insulin modified as its monomer behaved the same as the native
insulin. How
ever, when it was modified as the dimer, the product remained as
dimer under conditions where native
insulin could be converted to
monomer.
Advisors/Committee Members: Becker, Robert R. (advisor).
Subjects/Keywords: Insulin
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Manager
APA (6th Edition):
Wu, L. H. L. (1966). Preparation and properties of polyglycyl insulin. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47267
Chicago Manual of Style (16th Edition):
Wu, Lily Henson Liu. “Preparation and properties of polyglycyl insulin.” 1966. Masters Thesis, Oregon State University. Accessed February 27, 2021.
http://hdl.handle.net/1957/47267.
MLA Handbook (7th Edition):
Wu, Lily Henson Liu. “Preparation and properties of polyglycyl insulin.” 1966. Web. 27 Feb 2021.
Vancouver:
Wu LHL. Preparation and properties of polyglycyl insulin. [Internet] [Masters thesis]. Oregon State University; 1966. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1957/47267.
Council of Science Editors:
Wu LHL. Preparation and properties of polyglycyl insulin. [Masters Thesis]. Oregon State University; 1966. Available from: http://hdl.handle.net/1957/47267
University of Debrecen
6.
Németh, Anna.
A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
.
Degree: DE – TEK – Természettudományi és Technológiai Kar – Biológiai és Ökológiai Intézet, 2011, University of Debrecen
URL: http://hdl.handle.net/2437/108264
► A dolgozatban arra kerestem a választ, hogy mi történik a lipid anyagcserével diabetes mellitus során és azokat a tényezőket is, amelyeknek igazolódik a betegség létrejöttében…
(more)
▼ A dolgozatban arra kerestem a választ, hogy mi történik a lipid anyagcserével diabetes mellitus során és azokat a tényezőket is, amelyeknek igazolódik a betegség létrejöttében betöltött szerepe.
Nagyon fontos összefüggések derültek ki a zsíranyagcsere és cukorbetegség között és milyen tényezők okozhatnak cukorbetegséget, valamint,hogy mi a cukorbetegség és lipid anyagcsere fogalma, típusai, jellemzői.
Advisors/Committee Members: Vargha, György (advisor).
Subjects/Keywords: diabetes;
insulin
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APA (6th Edition):
Németh, A. (2011). A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/108264
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Németh, Anna. “A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
.” 2011. Thesis, University of Debrecen. Accessed February 27, 2021.
http://hdl.handle.net/2437/108264.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Németh, Anna. “A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
.” 2011. Web. 27 Feb 2021.
Vancouver:
Németh A. A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
. [Internet] [Thesis]. University of Debrecen; 2011. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2437/108264.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Németh A. A cukorbetegség és a lipid anyagcsere zavarainak összefüggései
. [Thesis]. University of Debrecen; 2011. Available from: http://hdl.handle.net/2437/108264
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Queens University
7.
Golkaran, Donya.
In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
.
Degree: Chemical Engineering, 2013, Queens University
URL: http://hdl.handle.net/1974/8211
► The aim of this study was to evaluate the factors, which affect insulin release and stability in simulated gastrointestinal fluid that ultimately will reduce the…
(more)
▼ The aim of this study was to evaluate the factors, which affect insulin release and stability in simulated gastrointestinal fluid that ultimately will reduce the bioavailability of insulin in a nanoparticulate oral delivery system. The focus was on nanoparticulate carriers developed by C. Reis, which have the highest level of bioavailability reported in the literature thus far.
Particles observed by TEM were spherical and particle analyzer data showed that the peak of the distribution was 10 nm. Entrapment efficiency of insulin was 85%. Insulin retention/release was evaluated in both enzymatic and enzyme-free simulated digestive fluids. HPLC measurement showed that insulin was stable in acid condition in presence and absence of pepsin. By changing the pH to 6.8 in an intestinal simulation, the amount of insulin decreased such that at the end of the 5 h simulation, 71% of the insulin was measureable in simulated GI fluid in presence of enzymes.
Insulin release profile from nanoparticles was low in gastric condition. After changing the pH to 6.8, an initial release of insulin occurred in the first 1h followed by plateau state in the remaining 2h. After a total of 5h in acidic followed by neutral pH medium, the formulation retained 48% of the insulin in the particles in simulated GI fluid in presence of enzyme. As confirmation of the amount of retained insulin, after 5h, particles were dissolved and the formulation was shown to fully retain up to 45% of the insulin in extended simulated gastrointestinal condition in the presence of enzymes.
Insulin release behavior was investigated in different simulated small intestinal media by incorporating phosphate buffer or bicarbonate buffer and physiological electrolytes. The release rate from particles in the phosphate buffer was faster compared to bicarbonate buffers. KBB-C showed a release profile that was very different from other media, with about 10% released in the first 30 min and 70% of the insulin remaining entrapped within the particles at the end of the experiment. Release in this buffer was reduced due to the decreased sodium to calcium ratio compared to the other KBB media.
Subjects/Keywords: Insulin Delivery
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Manager
APA (6th Edition):
Golkaran, D. (2013). In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
. (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/8211
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Golkaran, Donya. “In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
.” 2013. Thesis, Queens University. Accessed February 27, 2021.
http://hdl.handle.net/1974/8211.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Golkaran, Donya. “In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
.” 2013. Web. 27 Feb 2021.
Vancouver:
Golkaran D. In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
. [Internet] [Thesis]. Queens University; 2013. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/1974/8211.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Golkaran D. In Vitro Simulation of In Vivo Performance of Oral Dosed Nanoparticulate Insulin
. [Thesis]. Queens University; 2013. Available from: http://hdl.handle.net/1974/8211
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McGill University
8.
Lai, Wei H.
Characterization of growth Factor Receptors in Human Placental Cells in Culture.
Degree: PhD, Department of Experimental Medicine, 1984, McGill University
URL: https://escholarship.mcgill.ca/downloads/dv13zw87q.pdf
;
https://escholarship.mcgill.ca/concern/theses/41687k59b
► The characteristics and specificity of receptors for insulin, epidermal growth factor (EGF), and the insulin-like growth factors (IGFs), IGF-I and IGF-II, were studied in human…
(more)
▼ The characteristics and specificity of receptors for insulin, epidermal growth factor (EGF), and the insulin-like growth factors (IGFs), IGF-I and IGF-II, were studied in human early gestation and term placental cell cultures of syncytiotrophoblast origin. [125r]-1abeled peptides bound rapidly and reversibly to their cell surface receptors and exhibited a high degree of specificity. Maximal binding occurred at pH 7.5 and binding was proportional to cell protein and ligand concentrations. The placenta was found to be extremely rich in degrading activity for insulin and IGFs, but not for EGF. Bacitracin decreased the degradation of insulin and IGF peptides and was essential for the study of these peptides. […]
Les caractéristiques et spécificités des récepteurs à l'insuline au facteur de croissance épidermique (EGF), et aux facteurs de croissance insuliniques IGF-I et IGF-II, furent étudiés sur des cultures de cellules du syncytiotrophoblaste provenant de placentae de début de gestation et de placentae à terme. Les peptides marqués à l'iode 125 [125I] se liaient rapidement et réversiblement à leurs récepteurs membranaires et montraient un haut degré de spécificité. Le taux de liaison était maximal à pH 7.5 et était proportionnel à la concentration de proteine cellulaire et de chacun des peptides marqués. Il fut noté que le placenta possédait un pouvoir de dégradation de l'insuline et des IGFs extrêmement important, mais très faible dans le cas de l'EGF. La bacitracine diminuait la dégradation de l'insuline et des IGFs et était indispensable a l'étude de ces peptides. […]
Advisors/Committee Members: Guyda, H. J..
Subjects/Keywords: Insulin
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APA ·
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APA (6th Edition):
Lai, W. H. (1984). Characterization of growth Factor Receptors in Human Placental Cells in Culture. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/dv13zw87q.pdf ; https://escholarship.mcgill.ca/concern/theses/41687k59b
Chicago Manual of Style (16th Edition):
Lai, Wei H. “Characterization of growth Factor Receptors in Human Placental Cells in Culture.” 1984. Doctoral Dissertation, McGill University. Accessed February 27, 2021.
https://escholarship.mcgill.ca/downloads/dv13zw87q.pdf ; https://escholarship.mcgill.ca/concern/theses/41687k59b.
MLA Handbook (7th Edition):
Lai, Wei H. “Characterization of growth Factor Receptors in Human Placental Cells in Culture.” 1984. Web. 27 Feb 2021.
Vancouver:
Lai WH. Characterization of growth Factor Receptors in Human Placental Cells in Culture. [Internet] [Doctoral dissertation]. McGill University; 1984. [cited 2021 Feb 27].
Available from: https://escholarship.mcgill.ca/downloads/dv13zw87q.pdf ; https://escholarship.mcgill.ca/concern/theses/41687k59b.
Council of Science Editors:
Lai WH. Characterization of growth Factor Receptors in Human Placental Cells in Culture. [Doctoral Dissertation]. McGill University; 1984. Available from: https://escholarship.mcgill.ca/downloads/dv13zw87q.pdf ; https://escholarship.mcgill.ca/concern/theses/41687k59b
Deakin University
9.
Holmes-Truscott, Elizabeth.
Receptiveness and resistance: perceptions of insulin use in type 2 diabetes.
Degree: School of Psychology, 2016, Deakin University
URL: http://hdl.handle.net/10536/DRO/DU:30097451
► The aim of this thesis was to investigate attitudes to insulin therapy among Australian adults with type 2 diabetes. Using a mixed methods approach, this…
(more)
▼ The aim of this thesis was to investigate attitudes to
insulin therapy among Australian adults with type 2 diabetes. Using a mixed methods approach, this research identified barriers and facilitators of
insulin initiation, ongoing use, and intensification, as well as factors associated with resistance or receptiveness to
insulin therapy.
Advisors/Committee Members: Speight, Jane, Ricciardelli, Lina.
Subjects/Keywords: psychological insulin resistance (PIR); diabetes; insulin therapy
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Holmes-Truscott, E. (2016). Receptiveness and resistance: perceptions of insulin use in type 2 diabetes. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30097451
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Holmes-Truscott, Elizabeth. “Receptiveness and resistance: perceptions of insulin use in type 2 diabetes.” 2016. Thesis, Deakin University. Accessed February 27, 2021.
http://hdl.handle.net/10536/DRO/DU:30097451.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Holmes-Truscott, Elizabeth. “Receptiveness and resistance: perceptions of insulin use in type 2 diabetes.” 2016. Web. 27 Feb 2021.
Vancouver:
Holmes-Truscott E. Receptiveness and resistance: perceptions of insulin use in type 2 diabetes. [Internet] [Thesis]. Deakin University; 2016. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10536/DRO/DU:30097451.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Holmes-Truscott E. Receptiveness and resistance: perceptions of insulin use in type 2 diabetes. [Thesis]. Deakin University; 2016. Available from: http://hdl.handle.net/10536/DRO/DU:30097451
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
10.
Cvijović, Goran M., 1971-.
Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma.
Degree: Medicinski fakultet, 2016, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:12592/bdef:Content/get
► INTERNA MEDICINA - ENDOKRINOLOGIJA / INTERNAL MEDICINE - ENDOCRINOLOGY
Uvod i cilj: Prethodno je uočeno postojanje insulinske rezistencije i povećana prevalenca oštećene tolerancije na glikozu…
(more)
▼ INTERNA MEDICINA - ENDOKRINOLOGIJA / INTERNAL
MEDICINE - ENDOCRINOLOGY
Uvod i cilj: Prethodno je uočeno postojanje
insulinske rezistencije i povećana prevalenca oštećene tolerancije
na glikozu i tipa 2 dijabetesa kod pacijenata sa primarnim
hiperparatireoidizmom. Efekat paratireoidektomije na insulinsku
senzitivnost je praćen kontraverznim nalazima u zavisnosti od
metode koja je korištena za procenu insulinske senzitivnosti: nije
bilo promene kada su korišteni HOMA i QUICKI dok je Minimalnim
modelom pokazano značajno poboljšanje insulinske senzitivnosti.Cilj
ovog rada je bio da se proceni insulinska senzitivnost pre i nakon
paratireoidektomije kod pacijenata sa PHPT putem euglikemijskog
hiperinsulinemijskog klampa. Materijal i metode: Kod 44 pacijenta
sa PHPT i 11 zdravih kontrola komparabilne starosti i indeksa
telesne mase procenjivali smo insulinsku senzitivnost (pomoću
tehnike klampa, HOMA IR i ISI indeksa), insulinsku sekreciju (putem
površine pod krivom za glikemije i insulinemije tokom OGTTa i
izračunavajući akutni insulinski odgovor iz podataka dobijenih
IVGTTom), lipidni profil i nivo hsCRP kao marker hronične
inflamacije. Rezultati: Pre operacije vrednosti M indeksa i HOMA IR
su ukazivale na postojanje insulinske rezistencije kod pacijenata
sa PHPT. Nije bilo razlike u M indeksu (3.74±1.89 vs 4.62±2.27, p
>0.05), HOMA IR (2.94±1.39 vs. 3.29±0.81, p > 0.05), AUC
glikoze (863.0±261.3 vs 842.3±165.5, p>0.05), AUC insulina
(7068.7±4159.0 vs 7229.6±2581.7, p>0.05), ISI (4.73±2.77 vs
4.25±2.94, p>0.05) i AIR (47.89±32.05 vs. 38.96±21.20,
p>0.05) izmeĎu pacijenata sa PHPT i zdravih kontrola. Došlo je
do značajnog poboljšanja insulinske senzitivnosti nakon operacije
al ii preoperativna i postoperativna vrednost M indeksa se nisu
razlikovale od zdravih kontrola. Nije uočena promena u HOMA IR, AUC
za glikozu i insulin ISI i AIR, lipidima i hsCRP nakon operacije.
Zaključak: Dobili smo značajno poboljšanje insulinske senzitivnosti
nakon paratireoidektomije kod pacijenata sa PHPT koristeći klamp
tehniku. Postoperativno nije do došlo do značajne promene u drugim
pokazateljima insulinske senzitivnosti, paramtrima insulinske
sekrecije, lipidima i hsCRP.
Advisors/Committee Members: Micić, Dragan, 1950-.
Subjects/Keywords: primary hyperparathyroidism; insulin sensitivity;
insulin secretion
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Cvijović, Goran M., 1. (2016). Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:12592/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cvijović, Goran M., 1971-. “Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma.” 2016. Thesis, Univerzitet u Beogradu. Accessed February 27, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:12592/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cvijović, Goran M., 1971-. “Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma.” 2016. Web. 27 Feb 2021.
Vancouver:
Cvijović, Goran M. 1. Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Feb 27].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:12592/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cvijović, Goran M. 1. Sekrecija insulina i insulinska senzitivnost perifernih
tkiva pre i nakon radikalnog lečenja primarnog
hiperparatireoidizma. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:12592/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Louisiana State University
11.
Lestelle, Jeanne D.
Insulin dose response curves and factors affecting insulin sensitivity in horses.
Degree: MS, Animal Sciences, 2012, Louisiana State University
URL: etd-04162012-115502
;
https://digitalcommons.lsu.edu/gradschool_theses/1016
► Three experiments were conducted to further study the use of intravenous insulin injection to measure insulin sensitivity in horses. In the first experiment, glucose…
(more)
▼ Three experiments were conducted to further study the use of intravenous insulin injection to measure insulin sensitivity in horses. In the first experiment, glucose responses to multiple doses of recombinant human insulin were compared in insulin sensitive and insensitive mares. Regression lines describing the responses in insensitive mares had less (P = 0.003) steep slopes and greater (P = 0.006) effective doses at 50% response (ED50) compared to sensitive mares. Curvilinear regression models provided a good fit (R2 = 0.95) for the prediction of ED50 from the individual responses to 50 mU/kg BW insulin. The second experiment estimated the repeatability of the glucose responses to a fixed dose of insulin in 41 horses (mares and geldings) injected twice, between 7 and 14 days apart. Overall agreement between the responses was significant (P < 0.001) but not high enough to be predictive (R2 value = 0.384). Intra-horse coefficients of variation (CV) ranged from 0 to 68.7%, and averaged 23%. Mares and geldings were similar in their repeatabilities. Horses in the bottom half of the sensitivity rankings had a greater (P = 0.03) average intra-horse CV (28.7 ± 4.6%) than horses in the top half (16.7 ± 2.2%). In the third experiment (single switch-back design), glucose responses to a fixed dose of insulin were compared after an overnight (about 12 hours) versus a 24-hour period of feed deprivation in geldings previously determined to be insulin sensitive (n = 4) versus insensitive (n = 4). The longer period of feed deprivation decreased (P = 0.06) the percentage decrease in blood glucose concentrations, indicating a lesser sensitivity relative to the 12-hour period of deprivation. Insulin sensitivity also affected (P = 0.0003) the glucose response, with sensitive geldings exhibiting the greater response. It was concluded that the response to a single dose of insulin is often a good predictor of a horse's insulin sensitivity, but that the lack of repeatability in some horses could lead to errors in diagnosis. The decrease in glucose response seen after 24 hours of feed deprivation is likely due to the elevation of fatty acid concentrations in the blood.
Subjects/Keywords: insulin; horse; insulin sensitivity; glucose tolerance
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lestelle, J. D. (2012). Insulin dose response curves and factors affecting insulin sensitivity in horses. (Masters Thesis). Louisiana State University. Retrieved from etd-04162012-115502 ; https://digitalcommons.lsu.edu/gradschool_theses/1016
Chicago Manual of Style (16th Edition):
Lestelle, Jeanne D. “Insulin dose response curves and factors affecting insulin sensitivity in horses.” 2012. Masters Thesis, Louisiana State University. Accessed February 27, 2021.
etd-04162012-115502 ; https://digitalcommons.lsu.edu/gradschool_theses/1016.
MLA Handbook (7th Edition):
Lestelle, Jeanne D. “Insulin dose response curves and factors affecting insulin sensitivity in horses.” 2012. Web. 27 Feb 2021.
Vancouver:
Lestelle JD. Insulin dose response curves and factors affecting insulin sensitivity in horses. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Feb 27].
Available from: etd-04162012-115502 ; https://digitalcommons.lsu.edu/gradschool_theses/1016.
Council of Science Editors:
Lestelle JD. Insulin dose response curves and factors affecting insulin sensitivity in horses. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-04162012-115502 ; https://digitalcommons.lsu.edu/gradschool_theses/1016
University of Canterbury
12.
Subramanian, Kannan.
Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR).
Degree: PhD, Chemical Engineering, 2014, University of Canterbury
URL: http://dx.doi.org/10.26021/2927
► Type 2 diabetes or adult onset diabetes, has been a global epidemic for the past two decades, and the number of new cases accelerates every…
(more)
▼ Type 2 diabetes or adult onset diabetes, has been a global epidemic for the past two decades, and the number of new cases accelerates every year. Insulin resistance is one of the major factors behind this, wherein the insulin receptor, which signals to regulate glucose levels, based on the hormone insulin, loses its sensitivity. Obesity is one other major concern which is caused due to the improper balance between the caloric intake and the energy utilized. Gastric bypass surgeries (GBP) are performed to avert obesity. However, a beneficial side-effect is that the state of insulin resistance is reset to near baseline levels within a few days after the procedure. The reason behind this remains unexplained, with possible humoral effects, hypothesized to occur after the bariatric procedure.
In this work, high-five insect cell line was utilized to recombinantly produce full length insulin receptors (IR). However commercially sourced IR ectodomains (eIR – soluble version of the full length IR with the completely extracellular α subunits along with extracellular and transmembrane regions of the β subunit), were used to study the interaction. Measuring the kinetics of IR-insulin interactions is critical to improving our understanding of this disease. In this study, a multiplex surface plasmon resonance (SPR) assay was developed for studying the interaction between insulin and the eIR. A scaffold approach was used in which anti-insulin receptor monoclonal antibody 83–7 (Abcam, Cambridge, UK) was first immobilized on the SPR sensorchip by amine coupling, followed by eIR capture. The multiplex SPR system (ProteOn XPR36TM, Bio-Rad Laboratories, Hercules, CA) enabled measurement of replicate interactions with a single, parallel set of analyte injections, whereas repeated regeneration of the scaffold between measurements caused variable loss of antibody activity. The main approach was to replicate the physiological IR-insulin interaction using this assay. It was also observed that insulin at higher concentrations tend to form dimers and hexamers in solution.
This was tested using size exclusion chromatography analysis and proved to be true. Therefore an alternative analyte with the similar binding properties and affinity of insulin and at the same time with reduced self- association characteristics was explored. Lispro, the analogue of insulin with reduced self-association properties (generated by swapping of residue 28 and 29 with Lys and Pro respectively) was finally used to study the interaction with eIR.
Interactions between recombinant human insulin with eIR-A (A isoform of the insulin receptor ectodomain) followed a two-site binding pattern (consistent with the literature), with a high-affinity site (dissociation constant KD1 = 38.1 ± 0.9 nM) and a low-affinity site (KD2 = 166.3 ± 7.3 nM). The predominantly monomeric insulin analogue Lispro had corresponding dissociation constants KD1 =73.2 ± 1.8 nM and KD2 =148.9 ± 6.1 nM, but the fit to kinetic data was improved when conformational change factor was included in which…
Subjects/Keywords: insulin; insulin-receptor; kinetics; surface plasmon resonance
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Subramanian, K. (2014). Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR). (Doctoral Dissertation). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/2927
Chicago Manual of Style (16th Edition):
Subramanian, Kannan. “Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR).” 2014. Doctoral Dissertation, University of Canterbury. Accessed February 27, 2021.
http://dx.doi.org/10.26021/2927.
MLA Handbook (7th Edition):
Subramanian, Kannan. “Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR).” 2014. Web. 27 Feb 2021.
Vancouver:
Subramanian K. Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR). [Internet] [Doctoral dissertation]. University of Canterbury; 2014. [cited 2021 Feb 27].
Available from: http://dx.doi.org/10.26021/2927.
Council of Science Editors:
Subramanian K. Kinetics of insulin - insulin receptor interaction using a surface plasmon resonance (SPR). [Doctoral Dissertation]. University of Canterbury; 2014. Available from: http://dx.doi.org/10.26021/2927
University of British Columbia
13.
Verchere, Cameron Bruce.
Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995.
Degree: MS- MSc, Physiology, 1987, University of British Columbia
URL: http://hdl.handle.net/2429/26657
► The effect of varying concentrations of glucose or the gastrointestinal hormones, gastric inhibitory polypeptide (GIP) and somatostatin (SS-14), on the in vitro immunoreactive insulin (IRI)…
(more)
▼ The effect of varying concentrations of glucose or the gastrointestinal hormones, gastric inhibitory polypeptide (GIP) and somatostatin (SS-14), on the in vitro immunoreactive insulin (IRI) response to the parasympathetic neurotransmitter, acetylcholine (ACh) was investigated. The isolated, vascularly perfused rat pancreas was used in all experiments.
Acetylcholine (1.0 µM) did not stimulate IRI secretion in the presence of 2.2 mM glucose. However, in the presence of 4.4, 6.6, or 8.9 mM glucose, ACh (1.0 µM) potently stimulated IRI secretion (approximately fourfold). At a higher glucose concentration (17.8 mM), the IRI response to ACh was reduced. GIP also potentiated the IRI response to 1.0 µM ACh. This potentiation was most marked in the presence of 1.0 nM GIP, whereas the effect of concomitant infusion of 0.2 nM GIP and 1.0 µM ACh was only slightly greater than additive. SS-14 potently inhibited ACh-stimulated IRI secretion. These results demonstrated the glucose dependency of cholinergically stimulated IRI secretion, and that physiological levels of glucose and GIP increased B-cell sensitivity to cholinergic stimulation. It was suggested that the parasympathetic stimulation of IRI secretion associated with food intake could be affected by postprandial increases in glucose, GIP, and SS-14.
The idea that endogenously released somatostatin may have influenced glucose or GIP-stimulated IRI secretion was not supported by the present experiments, since neither glucose (8.9 mM) nor GIP (2.0 nM) were found to have a significant effect on the release of pancreatic somatostatin-like immunoreactivity (SLI). Both atropine (1.0 µM) and hexamethonlum (100 µM) inhibited the IRI response to ACh. This suggested that the parasympathetic stimulation of IRI secretion was mediated not only by muscarinic receptors on the B-cell, but also by nicotinic receptors on intrapancreatic ganglia. Neither atropine nor hexamethonlum had a significant effect on glucose- or GIP-stimulated IRI secretion, indicating that the IRI response to these stimuli was not mediated by cholinergic receptors.
Both SS-14 and the synthetic somatostatin analog SMS 201-995 (SMS; Sandostatin®) inhibited IRI secretion stimulated by 8.9 mM glucose, 2.0 nM GIP, or 1;.0 µM ACh, but not 17.8 mM glucose. The most potent inhibition by both SS-14 and SMS was observed in the presence of the weakest IRI stimuli (8.9 mM glucose and 1.0 µM ACh). These results suggested that the inhibitory effects of somatostatin on the B-cell could be overcome by the presence of strong stimuli. In addition, the inhibitory effects of the native hormone and the analog were found to be approximately equipotent (weight basis), indicating that the increased potency of SMS previously observed in vivo was due to its longer half-life in plasma, and not due to a more potent direct effect on the B-cell.
Subjects/Keywords: Insulin; Insulin resistance
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Verchere, C. B. (1987). Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/26657
Chicago Manual of Style (16th Edition):
Verchere, Cameron Bruce. “Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995.” 1987. Masters Thesis, University of British Columbia. Accessed February 27, 2021.
http://hdl.handle.net/2429/26657.
MLA Handbook (7th Edition):
Verchere, Cameron Bruce. “Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995.” 1987. Web. 27 Feb 2021.
Vancouver:
Verchere CB. Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995. [Internet] [Masters thesis]. University of British Columbia; 1987. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2429/26657.
Council of Science Editors:
Verchere CB. Control of insulin secretion from the perfused rat pancreas : effects of acetylcholine and a somatostatin analog, SMS 201-995. [Masters Thesis]. University of British Columbia; 1987. Available from: http://hdl.handle.net/2429/26657
Universiteit Utrecht
14.
Dopper, I.
Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients.
Degree: 2014, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/292970
► The relationship between diabetes and the risk for cancer development seems to be more and more confirmed. An important link between these two is the…
(more)
▼ The relationship between diabetes and the risk for cancer development seems to be more and more confirmed. An important link between these two is the hyperinsulinemia found both in patients with T1DM and patients with T2DM. T1DM patients cannot produce
insulin anymore and are therefore dependent on
insulin administration via injections. This causes an abnormal, evenly distributed
insulin concentration throughout the body; for tissues that normally receive low amounts of
insulin,
insulin administration can lead to hyperinsulinemia. T2DM patients have a reduced responsiveness to
insulin, the β-cells in the pancreas try to compromise for this by producing more, which results in hyperinsulinemia. (Vigneri et al., 2009). Hyperinsulinemia can lead to an increased risk for cancer formation. In this literature study the molecular aspects of the relationship between cancer risk and hyperinsulinemia will be reviewed, to clearly overview to what extent a malfunctioning in the
insulin system can lead to cancer formation.
Advisors/Committee Members: Dansen, T.B..
Subjects/Keywords: Insulin; Cancer; Diabetes
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dopper, I. (2014). Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/292970
Chicago Manual of Style (16th Edition):
Dopper, I. “Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients.” 2014. Masters Thesis, Universiteit Utrecht. Accessed February 27, 2021.
http://dspace.library.uu.nl:8080/handle/1874/292970.
MLA Handbook (7th Edition):
Dopper, I. “Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients.” 2014. Web. 27 Feb 2021.
Vancouver:
Dopper I. Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Feb 27].
Available from: http://dspace.library.uu.nl:8080/handle/1874/292970.
Council of Science Editors:
Dopper I. Diabetes, insulin and cancer risk
The molecular role of insulin signaling and hyperinsulinemia in cancer development and progression in diabetes patients. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/292970
Deakin University
15.
Holmes-Truscott, Elizabeth.
Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes.
Degree: 2016, Deakin University
URL: http://hdl.handle.net/10536/DRO/DU:30096807
► The aim of this thesis was to investigate attitudes to insulin therapy among Australian adults with type 2 diabetes. Using a mixed methods approach, this…
(more)
▼ The aim of this thesis was to investigate attitudes to
insulin therapy among Australian adults with type 2 diabetes. Using a mixed methods approach, this research identified barriers and facilitators of
insulin initiation, ongoing use, and intensification, as well as factors associated with resistance or receptiveness to
insulin therapy.
Advisors/Committee Members: Speight, Jane, Ricciardelli, Lina.
Subjects/Keywords: diabetes; insulin therapy
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Holmes-Truscott, E. (2016). Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30096807
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Holmes-Truscott, Elizabeth. “Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes.” 2016. Thesis, Deakin University. Accessed February 27, 2021.
http://hdl.handle.net/10536/DRO/DU:30096807.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Holmes-Truscott, Elizabeth. “Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes.” 2016. Web. 27 Feb 2021.
Vancouver:
Holmes-Truscott E. Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes. [Internet] [Thesis]. Deakin University; 2016. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10536/DRO/DU:30096807.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Holmes-Truscott E. Receptiveness and resistance: perceptions of insulin use in Type 2 diabetes. [Thesis]. Deakin University; 2016. Available from: http://hdl.handle.net/10536/DRO/DU:30096807
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Debrecen
16.
Chandan, Amar Paul Singh.
Diabetes Mellitus and the implications of modern insulin therapies
.
Degree: DE – Általános Orvostudományi Kar, 2014, University of Debrecen
URL: http://hdl.handle.net/2437/194936
► This thesis was written with the purpose of investigating the current litterature available on the subject of insulin analogues. Recently a plethora of molecularly modified…
(more)
▼ This thesis was written with the purpose of investigating the current litterature available on the
subject of
insulin analogues. Recently a plethora of molecularly modified
insulin analogs have been investigated and been introduced to the market with their benefit over older
insulin preparations not being as clear as one would believe initially. In this work i have summarized many recent trials involving novel
insulin preparations in an attempt to establish their use over older preparations that have been available on the market for decades.
Advisors/Committee Members: Pórszász, Róbert (advisor), University of Debrecen::Faculty of Medicine::Pharmacology and Pharmacotherapy (advisor).
Subjects/Keywords: Diabetes Mellitus;
Insulin
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APA (6th Edition):
Chandan, A. P. S. (2014). Diabetes Mellitus and the implications of modern insulin therapies
. (Thesis). University of Debrecen. Retrieved from http://hdl.handle.net/2437/194936
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chandan, Amar Paul Singh. “Diabetes Mellitus and the implications of modern insulin therapies
.” 2014. Thesis, University of Debrecen. Accessed February 27, 2021.
http://hdl.handle.net/2437/194936.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chandan, Amar Paul Singh. “Diabetes Mellitus and the implications of modern insulin therapies
.” 2014. Web. 27 Feb 2021.
Vancouver:
Chandan APS. Diabetes Mellitus and the implications of modern insulin therapies
. [Internet] [Thesis]. University of Debrecen; 2014. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/2437/194936.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chandan APS. Diabetes Mellitus and the implications of modern insulin therapies
. [Thesis]. University of Debrecen; 2014. Available from: http://hdl.handle.net/2437/194936
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tasmania
17.
St-Pierre, P.
Role of the microvasculature in the development of muscle insulin resistance.
Degree: 2010, University of Tasmania
URL: https://eprints.utas.edu.au/22258/1/whole_St-PierrePhilippe2010_thesis.pdf
► Type 2 diabetes is a disease characterised by a decrease in the sensitivity to insulin. Recently evidence has suggested an involvement of the microvasculature in…
(more)
▼ Type 2 diabetes is a disease characterised by a decrease in the sensitivity to insulin. Recently evidence has suggested an involvement of the microvasculature in the development of type 2 diabetes. Microvascular perfusion is increased by insulin and impairment of this response may contribute to muscle insulin resistance by limiting insulin and glucose delivery to skeletal muscle myocytes. This thesis focuses on the involvement of the microvascular perfusion in the development of insulin resistance.
Two different models were used to assess the impact of the microvascular perfusion on the insulin-mediated glucose disposal. Firstly, 4 weeks of high-fat diet (HFD model) feeding to rats was used as a pre-diabetic model of insulin resistance. Secondly, partial blockage of skeletal muscle microvascular beds with 15 μm diameter latex microspheres (MS model) was used to assess the effect of decreased muscle microvascular perfusion on insulin sensitivity.
In each model, insulin sensitivity was determined during hyperinsulinaemic isoglycaemic clamps in anaesthetized rats. Femoral arterial blood flow (FBF) and microvascular perfusion (assessed by the hindleg metabolism of infused 1-methylxanthine) were measured. Hindleg glucose uptake was determined from arteriovenous difference and FBF and muscle glucose uptake from [(14)C]-2-deoxyglucose uptake.
Insulin-mediated increases in femoral blood flow and glucose uptake were blunted in the HFD rats compared to those on the normal diet, while insulininduced microvascular perfusion was completely abolished. To see whether contraction responses were intact on the HFD, the functional response to electrical stimulation in the HFD rats was explored. The contraction-mediated increase in microvascular perfusion and glucose uptake was unaffected by the HFD.
In the MS model, microsphere occlusion of hindleg muscle microvasculature diminished microvascular perfusion as well as insulin-mediated glucose uptake, thus causing a vascular-derived acute state of insulin resistance. Next, the long-term effect of microsphere occlusion was assessed. Surprisingly, insulin responses following chronic microsphere occlusion were restored by 2 weeks of recovery post-injection. This may be explained by a rapid angiogenic response in occluded muscle vasculature.
Collectively, this thesis demonstrates two key findings that highlight the potential role of the microvasculature in the development of skeletal muscle insulin resistance: (i) that insulin- but not contraction-mediated microvascular responses are impaired in a pre-diabetic model of insulin resistance, and (ii) that acute physical reduction of microvascular flow impairs muscle insulin sensitivity. This furthers our understanding of the aetiology of type 2 diabetes and emphasizes the microvasculature as an interesting therapeutic target for the management of type 2 diabetes.
Subjects/Keywords: Insulin resistance; Diabetes
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APA (6th Edition):
St-Pierre, P. (2010). Role of the microvasculature in the development of muscle insulin resistance. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/22258/1/whole_St-PierrePhilippe2010_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
St-Pierre, P. “Role of the microvasculature in the development of muscle insulin resistance.” 2010. Thesis, University of Tasmania. Accessed February 27, 2021.
https://eprints.utas.edu.au/22258/1/whole_St-PierrePhilippe2010_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
St-Pierre, P. “Role of the microvasculature in the development of muscle insulin resistance.” 2010. Web. 27 Feb 2021.
Vancouver:
St-Pierre P. Role of the microvasculature in the development of muscle insulin resistance. [Internet] [Thesis]. University of Tasmania; 2010. [cited 2021 Feb 27].
Available from: https://eprints.utas.edu.au/22258/1/whole_St-PierrePhilippe2010_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
St-Pierre P. Role of the microvasculature in the development of muscle insulin resistance. [Thesis]. University of Tasmania; 2010. Available from: https://eprints.utas.edu.au/22258/1/whole_St-PierrePhilippe2010_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of New South Wales
18.
Chen, Daniel.
Insulin-sensitive Obesity.
Degree: Clinical School - St Vincent's Hospital, 2015, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/55745
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:38988/SOURCE01?view=true
► Introduction: While insulin resistance and obesity coexist, some obese individuals remain insulin-sensitive. We examined phenotypic and metabolic factors associated with insulin sensitivity in both muscle…
(more)
▼ Introduction: While
insulin resistance and obesity coexist, some obese individuals remain
insulin-sensitive. We examined phenotypic and metabolic factors associated with
insulin sensitivity in both muscle and liver in obese individuals. Methods: Sixty-four non-diabetic obese adults (29 males) underwent hyperinsulinaemic (15 and 80 mU/m2/min)-euglycaemic clamps with deuterated glucose. Top tertile subjects for glucose infusion rate during the high-dose
insulin clamp (GIRHI) were assigned Musclesen and those in the lower two tertiles were assigned Muscleres. Secondarily, top tertile subjects for endogenous glucose production (EGP) suppression during the low-dose
insulin clamp were deemed Liversen and the remainder Liverres. Clinical and laboratory parameters, muscle sympathetic nervous activity (MSNA) and visceral, subcutaneous, liver and pancreatic fat were compared.Results: Musclesen and Muscleres had similar body mass index and total fat (P ≥ 0.12), but Musclesen had lower HbA1c (P < 0.001) and systolic (P = 0.01) and diastolic (P = 0.03) blood pressure (BP). Despite similar subcutaneous fat (P = 0.83), Musclesen had lower visceral (P < 0.001) and liver (P < 0.001) fat. Liversen had lower visceral (P < 0.01) and liver (P < 0.01) fat and CRP (P=0.02) than Liverres. When subjects were grouped by both GIRHI and EGP suppression,
insulin sensitivity at either muscle or liver conferred apparent protection from the adverse metabolic features that characterized subjects
insulin-resistant at both sites. HDL-cholesterol, 1-hour glucose, systolic BP and triglycerides explained 54% of the variance in muscle
insulin sensitivity. In men (but not women), MSNA burst frequency correlated inversely with liver
insulin sensitivity (r = -0.53, P = 0.02) and positively with the C-reactive protein (CRP) and fibroblast growth factor (FGF)-19 (r = 0.57, P = 0.006 and r = -0.47, P = 0.03, respectively). Conclusions: Obese subjects who were
insulin-sensitive at muscle and/or liver exhibited favourable metabolic features, including lower BP, liver and visceral adiposity. Basal sympathetic nerve activity related to liver
insulin sensitivity in men, but not in women. MSNA associated with the circulating hepatokines CRP and FGF-19, suggesting a potential hepato-endocrine-autonomic axis. This study identifies factors associated with, and possibly contributing to,
insulin sensitivity in obesity.
Advisors/Committee Members: Greenfield, Jerry, Garvan Institute of Medical Research, Faculty of Medicine, UNSW, Chisholm, Don, Garvan Institute of Medical Research, Faculty of Medicine, UNSW.
Subjects/Keywords: Obesity; Insulin sensitivity
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APA (6th Edition):
Chen, D. (2015). Insulin-sensitive Obesity. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/55745 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:38988/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Chen, Daniel. “Insulin-sensitive Obesity.” 2015. Doctoral Dissertation, University of New South Wales. Accessed February 27, 2021.
http://handle.unsw.edu.au/1959.4/55745 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:38988/SOURCE01?view=true.
MLA Handbook (7th Edition):
Chen, Daniel. “Insulin-sensitive Obesity.” 2015. Web. 27 Feb 2021.
Vancouver:
Chen D. Insulin-sensitive Obesity. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2021 Feb 27].
Available from: http://handle.unsw.edu.au/1959.4/55745 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:38988/SOURCE01?view=true.
Council of Science Editors:
Chen D. Insulin-sensitive Obesity. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/55745 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:38988/SOURCE01?view=true
19.
Smith, Zachary K. F.
The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves.
Degree: MS, Animal Science, 2015, South Dakota State University
URL: https://openprairie.sdstate.edu/etd/1978
► A series of experiments were conducted to evaluate the influence of supplemental dietary chromium (0 or 400 ppb) on insulin responsiveness in finishing steers…
(more)
▼ A series of experiments were conducted to evaluate the influence of supplemental dietary chromium (0 or 400 ppb) on
insulin responsiveness in finishing steers (Exp 1. and Exp 2.), and energy status in newly weaned steers upon introduction to the feedlot (Exp 3.). To evaluate
insulin responsiveness, two models were used with finished steers of different ages: Exp 1. 12 steers; 24 mo old (BW=783 ± 29 kg); and Exp 2. 24 steers; 14 mo old (BW=625 ± 24 kg). A jugular infusion of
insulin (0.45 IU bovine
insulin/ kg of BW0.75, INS) was pulse dosed 2 h post-prandial. A jugular infusion of PBS (SHAM) was included in Exp. 2. Blood samples were collected at -30, 120, 195, 255, and 315 min) relative to the morning feed delivery after 84 d (Exp 1.) and 86 d (Exp 2.) on test diets. No diet x time interactions were detected for any plasma metabolites in old or young steers. In Exp 2., INS effectively cleared plasma glucose (GLS). Diet had no impact on changes in GLS in Exp 2. Diet had no impact (P > 0.20) on plasma concentrations of
insulin in either experiment. In Exp 2., there tended (P=0.10) to be a diet x
insulin interaction for post-infusion
insulin levels.
Insulin values were similar for all steers at (t195) and (t315) and elevated at (t255) for non-Cr fed steers subjected to INS. At t120, CrP caused greater (P < 0.05) plasma urea-N (PUN) concentrations when compared to CON in Exp 1. but not in Exp 2. Effects of Cr on PUN in Exp 1. may be due to improved
insulin sensitivity affecting AA uptake in the peripheral tissue of old, heavy steers. In Exp 3., there were no diet x day interactions (P>0.20) for GLS,
insulin, or PUN. The GLS and PUN levels were similar between diets (P > 0.20).
Insulin levels were higher (P0.20) between diets on d 5 and higher (P < 0.05) on d 12 in calves fed CrP. The shift in NEFA on d 12 coincided with a spike in
insulin levels. Both events occurred at the time that NEG intake was approaching the acclimated plateau and neither event impacted glucose status. The effective use of chromium in feedlot diets is likely dictated by age dependent differences in
insulin sensitivity, metabolite needs for growth, and the degree of stress that ultimately result in a chromium deficient state.
Advisors/Committee Members: Robbi H. Pritchard.
Subjects/Keywords: chromium; insulin; steers
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APA ·
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Manager
APA (6th Edition):
Smith, Z. K. F. (2015). The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves. (Masters Thesis). South Dakota State University. Retrieved from https://openprairie.sdstate.edu/etd/1978
Chicago Manual of Style (16th Edition):
Smith, Zachary K F. “The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves.” 2015. Masters Thesis, South Dakota State University. Accessed February 27, 2021.
https://openprairie.sdstate.edu/etd/1978.
MLA Handbook (7th Edition):
Smith, Zachary K F. “The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves.” 2015. Web. 27 Feb 2021.
Vancouver:
Smith ZKF. The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves. [Internet] [Masters thesis]. South Dakota State University; 2015. [cited 2021 Feb 27].
Available from: https://openprairie.sdstate.edu/etd/1978.
Council of Science Editors:
Smith ZKF. The Evaluation of Chromium Propionate Supplementation on Insulin Responsiveness in Finishing Steers and Energy Status in Newly Weaned Steer Calves. [Masters Thesis]. South Dakota State University; 2015. Available from: https://openprairie.sdstate.edu/etd/1978
University of Tasmania
20.
Wallis, Michelle G.(Michelle Gaye).
Interaction between the vascular and metabolic actions of insulin.
Degree: 2002, University of Tasmania
URL: https://eprints.utas.edu.au/22080/1/whole_WallisMichelleGaye2002_thesis.pdf
Subjects/Keywords: Insulin resistance; Insulin; Insulin
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APA ·
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APA (6th Edition):
Wallis, M. G. (. G. (2002). Interaction between the vascular and metabolic actions of insulin. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/22080/1/whole_WallisMichelleGaye2002_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wallis, Michelle G (Michelle Gaye). “Interaction between the vascular and metabolic actions of insulin.” 2002. Thesis, University of Tasmania. Accessed February 27, 2021.
https://eprints.utas.edu.au/22080/1/whole_WallisMichelleGaye2002_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wallis, Michelle G (Michelle Gaye). “Interaction between the vascular and metabolic actions of insulin.” 2002. Web. 27 Feb 2021.
Vancouver:
Wallis MG(G. Interaction between the vascular and metabolic actions of insulin. [Internet] [Thesis]. University of Tasmania; 2002. [cited 2021 Feb 27].
Available from: https://eprints.utas.edu.au/22080/1/whole_WallisMichelleGaye2002_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wallis MG(G. Interaction between the vascular and metabolic actions of insulin. [Thesis]. University of Tasmania; 2002. Available from: https://eprints.utas.edu.au/22080/1/whole_WallisMichelleGaye2002_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Georgia State University
21.
Kloc, Noreen.
Insulin Dynamic Measures and Weight Change.
Degree: MPH, Public Health, 2016, Georgia State University
URL: https://scholarworks.gsu.edu/iph_theses/437
► ABSTRACT Insulin Dynamic Measures and Weight Change By Noreen Kloc B.S. Computer Information Technology, Purdue University December 7, 2015 INTRODUCTION: Weight gain and obesity…
(more)
▼ ABSTRACT
Insulin Dynamic Measures and Weight Change
By
Noreen Kloc
B.S. Computer Information Technology, Purdue University
December 7, 2015
INTRODUCTION: Weight gain and obesity are risk factors for
insulin resistance that can lead to type 2 diabetes and cardiovascular disease; however, there is a complicated interplay between
insulin sensitivity (SI), fasting
insulin, acute
insulin response (AIR), and disposition index (DI) and the relationship of these dynamic measures with weight change is not well understood.
AIM: The aim of this study was to investigate the relationships between
insulin dynamic measures, SI, fasting
insulin, AIR, and DI, with weight change during a 5-years follow-up period in the multi-ethnic cohort of the
Insulin Resistance Atherosclerosis Study (IRAS).
METHODS: Data on 879 men and women of Hispanic, non-Hispanic White, and African-American race/ethnicity aged 40-69 years were obtained at baseline (1992-1994) and at 5 year follow-up. Crude associations between the
insulin dynamic measures and weight change were evaluated using Kruskal-Wallis test and the relationships between log-transformed
insulin-related variables were examined using Spearman rank-order analysis. Multivariate regression models evaluated associations of interest adjusted for age, sex, ethnicity, and diabetes status in a time-dependent manner using mixed models.
RESULTS:
Insulin sensitivity SI inversely coevolves with weight, i.e. greater weight is predicted by lower SI at any time point. To answer the question whether SI is the cause or a consequence of weight change, we examined the associations with the baseline values and a change in SI. In this model, both the baseline SI and change in SI were inversely correlated with weight gain. A similar approach showed that baseline values and change in fasting
insulin were directly associated with weight gain. Weight change over time was associated with AIR, i.e. increases in AIR and greater AIR at baseline predicted weight gain. We did not find strong relationships between DI and weight change.
DISCUSSION: These results suggest that
insulin sensitivity and
insulin secretion can modulate weight in a non-diabetic population.
Advisors/Committee Members: Dora Il'yasova, PhD, Ruiyan Luo, PhD.
Subjects/Keywords: insulin resistance; insulin sensitivity; insulin secretion; weight; metabolic syndrome; diabetes
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APA (6th Edition):
Kloc, N. (2016). Insulin Dynamic Measures and Weight Change. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/iph_theses/437
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kloc, Noreen. “Insulin Dynamic Measures and Weight Change.” 2016. Thesis, Georgia State University. Accessed February 27, 2021.
https://scholarworks.gsu.edu/iph_theses/437.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kloc, Noreen. “Insulin Dynamic Measures and Weight Change.” 2016. Web. 27 Feb 2021.
Vancouver:
Kloc N. Insulin Dynamic Measures and Weight Change. [Internet] [Thesis]. Georgia State University; 2016. [cited 2021 Feb 27].
Available from: https://scholarworks.gsu.edu/iph_theses/437.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kloc N. Insulin Dynamic Measures and Weight Change. [Thesis]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/iph_theses/437
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
22.
Huang, Huei-Jhen.
Influence of chemical conditions on human insulin fibril structure.
Degree: Master, Chemistry, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
► Many proteins can misfold to form non-native structures and induce aggregation. Previous studies had found that more than 20 kinds of proteins may cause specific…
(more)
▼ Many proteins can misfold to form non-native structures and induce aggregation. Previous studies had found that more than 20 kinds of proteins may cause specific diseases that under ineffective prevention and treatment, and greatly perplexed many patients and researchers. Either the amino acid sequences, or molecular size, or the folded three-dimensional conformation of these pathogenic proteins is quite different, all of them will lead to the formation of insoluble amyloid fibers after misfolding. Herein, we investigated the environmental changes by the alcohol contentãpH value and adding short peptides and dendrimers in solvent incubated with human
insulin. We used circular dichroism (CD) spectroscopyãfourier transform infrared spectroscopy (FT-IR) and fluoresce spectroscopy to obtain the structural transition of the
insulin, and gain the morphology information of fibril by atomic force microscopy (AFM). The results showed that the increasing percentage of alcohol or increasing carbon chain length of alcohols can cause a difference CD spectra. The surface morphology of
insulin fibers by AFM imaging indicated that either increasing the percentage of alcohol or increasing alcohol carbon chain length will inhibit
insulin fiber formation. This phenomenon was attributing to hydrophobic group expose to solution and solvent molecular may prevent nucleus to contact. And lowering the pH will undergo conformational change from α-helix to β-sheet form that causes the formation of amyloid plaques. This result may be attributed to Cl- that covered the surface charge of
insulin fibril, then weaken the original electrostatic repulsion among
insulin fibrils and result in their aggregation.
Advisors/Committee Members: Po-Chiao Lin (chair), Shu-chen Hsieh (committee member), Chai-Lin Kao (chair).
Subjects/Keywords: alcohol; amyloid; pH; peptide; insulin
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Manager
APA (6th Edition):
Huang, H. (2013). Influence of chemical conditions on human insulin fibril structure. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huang, Huei-Jhen. “Influence of chemical conditions on human insulin fibril structure.” 2013. Thesis, NSYSU. Accessed February 27, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huang, Huei-Jhen. “Influence of chemical conditions on human insulin fibril structure.” 2013. Web. 27 Feb 2021.
Vancouver:
Huang H. Influence of chemical conditions on human insulin fibril structure. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Feb 27].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huang H. Influence of chemical conditions on human insulin fibril structure. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
23.
Yang, Shu-Wei.
Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy.
Degree: Master, Chemistry, 2012, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0803112-155349
► In this report, electrostatic force microscopy (EFM), zeta-potential analyzer, circular dichroism Spectrophotometer and Fourier transform infrared spectroscopy are used to study the electrostatic property of…
(more)
▼ In this report, electrostatic force microscopy (EFM), zeta-potential analyzer, circular dichroism Spectrophotometer and Fourier transform infrared spectroscopy are used to study the electrostatic property of surface on
insulin fibril. EFM provides a simultaneous probe of topography and electrostatic property with highly local resolution in nanometer-scale. To understand the correlation between charge distribution on fibril surface and structure transformation, we controlled the incubation time and salt concentration. The results show that the surface charge increases with incubation time, and with the salt concentration increased, the pitch is found to decrease, variation of charge distribution are increased. In addition, we also have evaluated influence of amino acid sequence on growth rate of fibril associated with bovine, porcine and human
insulin by atomic force microscopy and circular dichroism Spectrophotometer. The experimental results show that the number of hydrophilic groups on the peptide sequence will affect the speed of fibril generated, and the location of hydrophilic groups on the peptide sequence will affect the stability of the fibril under the frozen environment.
Advisors/Committee Members: Shuchen Hsieh (committee member), Chao-Ming Chiang (chair), Hui-Ting Chen (chair).
Subjects/Keywords: freezing; salt; EFM; amyloid; insulin
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Manager
APA (6th Edition):
Yang, S. (2012). Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0803112-155349
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yang, Shu-Wei. “Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy.” 2012. Thesis, NSYSU. Accessed February 27, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0803112-155349.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yang, Shu-Wei. “Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy.” 2012. Web. 27 Feb 2021.
Vancouver:
Yang S. Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy. [Internet] [Thesis]. NSYSU; 2012. [cited 2021 Feb 27].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0803112-155349.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yang S. Various insulin fibril surface charge distribution studied by Electrostatic Force Microscopy. [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0803112-155349
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
24.
Chang, Chiung-Wen.
Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope.
Degree: Master, Chemistry, 2011, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802111-124018
► We study the conformational change of insulin fibril growth from three aspects: the impact of (i) incubation time; (ⅱ) nano-particles; (iii) and ion added. We…
(more)
▼ We study the conformational change of
insulin fibril growth from three aspects: the impact of (i) incubation time; (ⅱ) nano-particles; (iii) and ion added. We used circular dichroism (CD) spectroscopy and fourier transform infrared spectroscopy (FT-IR) to obtain the structural transition of the
insulin, and gain the morphology information of fibril by atomic force microscopy (AFM) and transmission electron microscopy (TEM). We show that the
insulin transform from α-helix to β-sheet structure as increased incubated time. The addition of Au nanoparticles (NPs) caused the formation of coordination bond with
insulin fiber and produced shorter and thicker
insulin fibril . The Fe3O4 NPs, on the other hand, offered only van der Waals interaction toward
insulin fibril. Hence they could be used to separate
insulin fibril from solution. Finally, addition of salts can induce the conformation changes of
insulin fibril ten times faster than that without salts. And the
insulin fibril fragment was two or three times shorter than that produced without salts. At high salt concentration,
insulin formed amorphous aggregates. This phenomenon was attribute to anions from salt: covering the surface charge of
insulin fibril, they weaken the original electrostatic repulsion among
insulin fibrils and result in their aggregation.
Advisors/Committee Members: CHAO-MING CHIANG (chair), Michael Y. Chiang (chair), Shuchen Hsieh (committee member).
Subjects/Keywords: salt; nanoparticle; AFM; amyloid; Insulin
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APA (6th Edition):
Chang, C. (2011). Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802111-124018
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chang, Chiung-Wen. “Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope.” 2011. Thesis, NSYSU. Accessed February 27, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802111-124018.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chang, Chiung-Wen. “Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope.” 2011. Web. 27 Feb 2021.
Vancouver:
Chang C. Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Feb 27].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802111-124018.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chang C. Investigation of the Insulin Amyloid Fibrils Structural Information by Atomic Force Microscope. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0802111-124018
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Dalhousie University
25.
Nelson, Stephanie.
Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides.
Degree: MS, Department of Physiology & Biophysics, 2010, Dalhousie University
URL: http://hdl.handle.net/10222/13014
► Proghrelin produces three proghrelin derived peptides (PGDP) known for their roles in glucose homeostasis: acylated ghrelin (AG), unacylated ghrelin (UAG), and obestatin (Ob). Only the…
(more)
▼ Proghrelin produces three proghrelin derived peptides
(PGDP) known for their roles in glucose homeostasis: acylated
ghrelin (AG), unacylated ghrelin (UAG), and obestatin (Ob). Only
the receptor for AG, growth hormone secretagogue receptor 1a (GHS
R1a), is known, however there is evidence for additional receptors
for AG, UAG and Ob. Our aim was to determine the actions of PGDP on
two beta cell lines, MIN6 and INS-1, which we have shown to contain
and lack GHS R1a respectively. PGDP increased proliferation in
INS-1 but not MIN6 cells, measured by BrdU incorporation. AG
decreased apopotosis in both cell lines, measured by decreased
levels of activated caspase 3.
Insulin secretion was investigated
in INS-1 cells, where PGDP modulated
insulin release in a glucose
dependent manner. Our results indicate that PGDP modulate beta cell
mass in the presence and absence of GHS R1a, and present a detailed
anaylsis of
insulin secretion in INS-1 cells.
Advisors/Committee Members: Dr. Kerry Goralski (external-examiner), Dr. Elizabeth Cowley (graduate-coordinator), Dr. Valerie Chappe (thesis-reader), Dr. Ali Imran (thesis-reader), Dr. Paul Murphy (thesis-reader), Dr. Younes Anini (thesis-supervisor), Not Applicable (ethics-approval), Not Applicable (manuscripts), Not Applicable (copyright-release).
Subjects/Keywords: Ghrelin; Diabetes; insulin; obestatin
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APA (6th Edition):
Nelson, S. (2010). Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/13014
Chicago Manual of Style (16th Edition):
Nelson, Stephanie. “Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides.” 2010. Masters Thesis, Dalhousie University. Accessed February 27, 2021.
http://hdl.handle.net/10222/13014.
MLA Handbook (7th Edition):
Nelson, Stephanie. “Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides.” 2010. Web. 27 Feb 2021.
Vancouver:
Nelson S. Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides. [Internet] [Masters thesis]. Dalhousie University; 2010. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10222/13014.
Council of Science Editors:
Nelson S. Regulation of Pancreatic Beta Cell Mass and Function by
Proghrelin Derived Peptides. [Masters Thesis]. Dalhousie University; 2010. Available from: http://hdl.handle.net/10222/13014
University of Utah
26.
Taylor, Joseph Charles.
Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;.
Degree: PhD, Biochemistry;, 1973, University of Utah
URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1546/rec/1132
► The relationship of fibrinolytic activity produced by baby hamster kidney (BHK-21) cells to surface properties and growth of the cells has been studied. Hydrolysis of…
(more)
▼ The relationship of fibrinolytic activity produced by
baby hamster kidney (BHK-21) cells to surface properties and growth
of the cells has been studied. Hydrolysis of fibrin clots and
casein was used to detect cellular protease activity. Cloned, high
passage sublines of the BHK-21/C13 cell line were found to produce
increased fibrinolysis when compared to low passage BHK-21/C13
cells (C13). Low passage C13 cells were also found to produce an
inhibitor of the cellular fibrinolysin. This inhibitory activity
was detected when low and high protease-producing cell lines were
co-cultivated and could be removed from the C13 cells by trypsin
treatment. Inhibitor could again be detected in C13 cell cultures
after 6-8.5 hours incubation at 37°C following typsinization. This
substance was not detected in supernatant fluids from growing C13
cells and its production was inhibited by incubation of cells at
4°C. All high protease-producing cultures formed colonies in agar
suspension, exhibited quantitative increases in agglutinability by
concanavalin A (Con A) when compared to low passage cell cultures
and grew faster as judged by transfer behavior of cells and
increased rates of accumulation of cells in metaphase when grown in
the presence of mitotic inhibitor. Low protease-producing C13 cells
and sublines low in protease activity isolated from high passage
cultures did not grow in agar suspension, exhibited slower growth
rates and decreased agglutinability by Con A. Low
protease-producing C13 cells could be stimulated to produce
increase amounts of fibrinolytic activity with accompanying changes
in surface properties characteristic of transformed cells by
addition of purines such as hypoxanthine or adenosine to culture
media. The effect was reversed by deletion of the purines. Growth
of C13 cell in agar suspension did not occur with purine
supplementation alone. With the addition of hypoxanthine and
thymidine or adenosine and thymidine or adenosine and thymidine,
C13 cells formed colonies in agar suspension which were similar to
those formed by high protease-producing cell lines. Fibrinolytic
activity could be detected from the colonies formed by C13 cells in
agar suspension. Treatment of C13 cells suspended in agar with
trypsin or fibrinolytically active culture fluids from high
protease-producing cells did not result in the multiplication of
the cells in agar. Temperature sensitive BHK-21 cells were isolated
which were defective for cytokinesis but not karyokinesis. At
39-40°C these cells became enlarged and multinucleated. The defect
did not appear to be associated with the cellular fibrinolysin
because protease activity could be detected at the permissive and
non-permissive temperatures. Growth of temperature sensitive cells
in agar suspension did not occur at 39-40°C but cell enlargement,
vacillation and lysis were observed in a large portion of the cell
population. The data demonstrate that growth in agar suspension,
increased Con A agglutinability and increased growth rates are
positively associated in some way with the…
Subjects/Keywords: Insulin; Proteins
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APA ·
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Manager
APA (6th Edition):
Taylor, J. C. (1973). Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1546/rec/1132
Chicago Manual of Style (16th Edition):
Taylor, Joseph Charles. “Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;.” 1973. Doctoral Dissertation, University of Utah. Accessed February 27, 2021.
http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1546/rec/1132.
MLA Handbook (7th Edition):
Taylor, Joseph Charles. “Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;.” 1973. Web. 27 Feb 2021.
Vancouver:
Taylor JC. Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;. [Internet] [Doctoral dissertation]. University of Utah; 1973. [cited 2021 Feb 27].
Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1546/rec/1132.
Council of Science Editors:
Taylor JC. Relationship of fibrinolytic activity to the growth of baby
hamster kidney cells in vitro;. [Doctoral Dissertation]. University of Utah; 1973. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1546/rec/1132
Tulane University
27.
Morford, Jamie.
Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females.
Degree: 2020, Tulane University
URL: https://digitallibrary.tulane.edu/islandora/object/tulane:120519
► [email protected]
Androgen excess predisposes females to type 2 diabetes. Using mouse models, our lab reported that androgen excess causes insulin resistance via activation of the…
(more)
▼ [email protected]
Androgen excess predisposes females to type 2 diabetes. Using mouse models, our lab reported that androgen excess causes insulin resistance via activation of the androgen receptor (AR) in the brain. Neurons of the arcuate nucleus of the hypothalamus (ARC) regulate hepatic glucose production (HGP). Thus, I hypothesized that in female mice, androgen excess in neurons of the ARC causes hepatic insulin resistance by increasing HGP. To test this, I injected AaV-Cre-GFP or AaV-GFP into the ARC of ARlox/lox female mice to generate ARC-specific AR knockout (ARC-ARKO) and control mice, respectively. When exposed to a Western diet, control female mice chronically treated with dihydrotestosterone (DHT) developed insulin resistance and fasting hyperglycemia compared to vehicle-treated control mice. In contrast, DHT-treated ARC-ARKO mice remained insulin sensitive and normoglycemic compared to vehicle-treated ARC-ARKO mice. During a hyperinsulinemic-euglycemic clamp, insulin’s ability to suppress HGP was blunted in DHT-treated control mice. In contrast, insulin was still able to suppress HGP in DHT-treated ARC-ARKO females. Additionally, during the clamp, DHT-treated control mice showed no alteration in hepatic activation of AKT, a marker of hepatocyte insulin sensitivity, but exhibited reduced activation of hepatic STAT3, a marker of hypothalamic insulin sensitivity. In contrast, in DHT-treated ARC-ARKO mice activation of hepatic STAT3 was increased. In a parallel study, estradiol treatment improved insulin sensitivity in control ovariectomized (OVX) mice. In contrast, in DHT-treated OVX mice, estradiol treatment did not improve insulin sensitivity. Together these results suggest that in female mice exposed to a Western diet, androgen excess causes hypothalamic estrogen resistance and insulin resistance in ARC neurons via action at the AR leading to impairments in the brain-IL6-pSTAT3 pathway which results in unsuppressed HGP.
1
Jamie Morford
Advisors/Committee Members: Mauvais-Jarvis, Franck (Thesis advisor), Daniel, Jill (Thesis advisor), Tasker, Jeff (Thesis advisor), Zsombok, Andrea (Thesis advisor), School of Science & Engineering Neuroscience (Degree granting institution).
Subjects/Keywords: Neuroscience; Androgen; Insulin Resistance
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APA (6th Edition):
Morford, J. (2020). Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:120519
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Morford, Jamie. “Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females.” 2020. Thesis, Tulane University. Accessed February 27, 2021.
https://digitallibrary.tulane.edu/islandora/object/tulane:120519.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Morford, Jamie. “Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females.” 2020. Web. 27 Feb 2021.
Vancouver:
Morford J. Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females. [Internet] [Thesis]. Tulane University; 2020. [cited 2021 Feb 27].
Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120519.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Morford J. Excess androgen acts via the androgen receptor (ar) in the arcuate nucleus of the hypothalamus (arc) to cause insulin resistance in females. [Thesis]. Tulane University; 2020. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120519
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
28.
Hu, Xiaobin.
Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans.
Degree: MS, Department of Biochemistry, 2010, University of Alberta
URL: https://era.library.ualberta.ca/files/xp68kg95d
► Mitochondrial dysfunction is considered as a key mechanism of aging but little is known about the impact of mitochondrial biogenesis. Mitochondrial DNA (mtDNA) copy number…
(more)
▼ Mitochondrial dysfunction is considered as a key
mechanism of aging but little is known about the impact of
mitochondrial biogenesis. Mitochondrial DNA (mtDNA) copy number
control is an important aspect of mitochondrial biogenesis and is
highly regulated in eukaryotic organisms. By studying mtDNA copy
number, our aim is to gain a better understanding of the
relationship between mitochondrial biogenesis and aging. We
developed an optimized protocol for measuring mtDNA copy number in
Caenorhabditis elegans using quantitative real-time PCR (qPCR). We
investigated how mtDNA regulation is affected by a variety of
aging-related pathways. We found the insulin/IGF-1 signaling (IIS)
pathway regulates mtDNA content in a DAF-16- and UCP-4-dependent
manner. By utilizing RNA interference (RNAi) against polg-1, we
showed that mitochondrial stress likely modulates lifespan through
the IIS pathway. Our work identifies IIS as a communications
pathway between mitochondria and the nucleus in modulating
mitochondrial biogenesis and lifespan in Caenorhabditis
elegans.
Subjects/Keywords: aging; mitochondria; insulin signaling
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Manager
APA (6th Edition):
Hu, X. (2010). Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/xp68kg95d
Chicago Manual of Style (16th Edition):
Hu, Xiaobin. “Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans.” 2010. Masters Thesis, University of Alberta. Accessed February 27, 2021.
https://era.library.ualberta.ca/files/xp68kg95d.
MLA Handbook (7th Edition):
Hu, Xiaobin. “Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans.” 2010. Web. 27 Feb 2021.
Vancouver:
Hu X. Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans. [Internet] [Masters thesis]. University of Alberta; 2010. [cited 2021 Feb 27].
Available from: https://era.library.ualberta.ca/files/xp68kg95d.
Council of Science Editors:
Hu X. Insulin signaling, mitochondrial DNA copy number regulation
and aging in Caenorhabditis elegans. [Masters Thesis]. University of Alberta; 2010. Available from: https://era.library.ualberta.ca/files/xp68kg95d
University of Alberta
29.
Rodriguez Dimitrescu, Carla.
Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD).
Degree: PhD, Department of Agricultural, Food, and Nutritional
Science, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/05741s17q
► Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease that typically occurs in obese children and adolescents with hyperinsulinemia/insulin resistance and dyslipidemia. While treatment…
(more)
▼ Nonalcoholic fatty liver disease (NAFLD) is a chronic
liver disease that typically occurs in obese children and
adolescents with hyperinsulinemia/insulin resistance and
dyslipidemia. While treatment is aimed at lifestyle modification
(diet and physical activity), very little is known regarding the
contribution of diet to underlying metabolic processes leading to
hepatic damage and how modification of meal patterning might
contribute to improved patient outcomes. We hypothesized that
children and adolescents with biopsy proven NAFLD will be
characterized by different body fat distribution, prolonged
postprandial hyperinsulinemia, lipemia and altered lipoprotein and
inflammatory marker expression when compared to obese and lean
healthy children and adolescents after consumption of two different
high saturated fat meal challenges (with varying long chain
polyunsaturated fat: 0% LCPUFA vs 1.5% LCPUFA). Children and
adolescents with NAFLD had higher fat localized to the visceral
region compared to the other groups. Higher (p>0.05) trunk
to extremity ratio and waist to hip ratio was observed in children
with NAFLD. Fasting and postprandial insulin were higher
(p<0.05) in the NAFLD when compared to lean and obese
controls. NEFA postprandial clearance in NAFLD population was
significantly lower when compared to obese and lean controls. NAFLD
subjects were characterized by higher (p<0.05) fasting Apo
B-100 and C-III as well as higher postprandial Apo B-48; all
suggestive of differences in fat balance across the liver following
a meal. Acute changes in LCPUFA intake in a high saturated fat meal
resulted in significant lowering of iAUC for NEFA in the NAFLD
group and significant increased postprandial changes in Apo C-III,
but did not evoke any other major postprandial changes in
lipoprotein expression. In conclusion our study suggests that
children and adolescents with NAFLD exhibit delayed postprandial
lipid and lipoprotein expression in comparison to lean controls.
Acute changes in LCPUFA content in a high saturated meal,
reflective of the typical amounts present in a fast food meal,
evoke small postprandial changes in children and adolescents with
NAFLD. Whether an improvement in postprandial lipid clearance can
be obtained through a higher LCPUFA dose or whether responses are
mainly determined by the chronic intake should be further
investigated
Subjects/Keywords: Body composition; Insulin resistance; NAFLD
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rodriguez Dimitrescu, C. (2012). Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD). (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/05741s17q
Chicago Manual of Style (16th Edition):
Rodriguez Dimitrescu, Carla. “Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD).” 2012. Doctoral Dissertation, University of Alberta. Accessed February 27, 2021.
https://era.library.ualberta.ca/files/05741s17q.
MLA Handbook (7th Edition):
Rodriguez Dimitrescu, Carla. “Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD).” 2012. Web. 27 Feb 2021.
Vancouver:
Rodriguez Dimitrescu C. Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD). [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Feb 27].
Available from: https://era.library.ualberta.ca/files/05741s17q.
Council of Science Editors:
Rodriguez Dimitrescu C. Influence of dietary fat intake on acute changes in
postprandial lipid and lipoprotein expression in children and
adolescents with nonalcoholic fatty liver disease (NAFLD). [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/05741s17q
Nelson Mandela Metropolitan University
30.
Clarke, Stephen.
Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens.
Degree: Faculty of Science, 2014, Nelson Mandela Metropolitan University
URL: http://hdl.handle.net/10948/d1020788
► Type 2 diabetes mellitus (T2DM) is rapidly emerging as one of the greatest global health issues of the 21st century. Insulin-resistance is a condition associated…
(more)
▼ Type 2 diabetes mellitus (T2DM) is rapidly emerging as one of the greatest global health issues of the 21st century. Insulin-resistance is a condition associated with T2DM and in the cell it is defined as the inadequate strength of insulin signalling from the insulin receptor downstream to the final substrates of insulin action involved in multiple metabolic, gene expression, and mitogenic aspects of cellular function. To investigate the potential mechanisms involved in the development of insulin-resistance, two in vitro liver cell models were established using palmitate or a combination of insulin and fructose as inducers. The development of insulin-resistance was determined via the capacity of the hepatocytes to maintain normal glucose metabolism functionality by measuring hepatic gluconeogenesis and glycogenolysis. It was established that the treatments induced the development of insulinresistance after 24 hours chronic exposure. Previous studies have investigated the potential of Sutherlandia frutescens extracts as therapeutic agents for insulin-resistance. The aim of this study was thus to investigate the ability of a hot aqueous extract of S. frutescens to reverse the insulin-resistant state, via measuring gluconeogenesis and glycogenolysis, the associated changes in cellular physiology (lipid accumulation, oxidative stress, and acetyl- CoA levels), and changes in mRNA expression. The results showed that S. frutescens had a significant effect on reversing the insulin-resistant state in both models of insulin-resistance. Furthermore, S. frutescens was capable of reducing lipid accumulation in the form of triacylglycerol in the high insulin/fructose model, while this was unaffected in the palmitate model. However, S. frutescens did reduce the accumulation of diacylglycerol in the palmitate model. Oxidative stress, seen to be associated with the insulin-resistant state, was successfully treated using the extract, as indicated by a reduction in reactive oxygen species. However no change was seen in the nitric oxide levels, in either model. Interestingly, although S. frutescens had no effect on the level of acetyl-CoA in the insulin/fructose model, it was found to increase this in the palmitate model. It is suggested that this may be due to increased β-oxidation and metabolic activity induced by the extract. The analysis of mRNA expression gave some insight into possible mechanisms by which insulin-resistance develops, although the results were inconclusive due to high variability in samples and the possibility of the RNA being compromised. Future studies will address this issue. The results of this study reflect different proposed clinical causes of insulin-resistance through the responses seen in the two cell models. These indicate that liver steatosis and insulin-resistance are induced by high palmitate as well as high insulin and fructose levels, and reversed by S. frutescens. Therefore the potential of S. frutescens to be used as a therapeutic agent in the treatment of insulin-resistance is indicated by this…
Subjects/Keywords: Insulin resistance; Diabetes – Treatment
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Manager
APA (6th Edition):
Clarke, S. (2014). Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/d1020788
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Clarke, Stephen. “Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens.” 2014. Thesis, Nelson Mandela Metropolitan University. Accessed February 27, 2021.
http://hdl.handle.net/10948/d1020788.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Clarke, Stephen. “Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens.” 2014. Web. 27 Feb 2021.
Vancouver:
Clarke S. Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2014. [cited 2021 Feb 27].
Available from: http://hdl.handle.net/10948/d1020788.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Clarke S. Liver steatosis and insulin-resistance : reversal by Sutherlandia frutescens. [Thesis]. Nelson Mandela Metropolitan University; 2014. Available from: http://hdl.handle.net/10948/d1020788
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations