subject:(induced pluripotent stem cells)

1. Paredes, Maria Guadalupe. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.

Degree: Biomedical Engineering, 2018, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:792836/

► Ischemic heart disease is the leading cause of death globally, which takes more than 17.7 million lives every year (WHO, 2018), yet current treatments and… (more)

Subjects/Keywords: induced pluripotent stem cells

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APA (6^th Edition):

Paredes, M. G. (2018). Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792836/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paredes, Maria Guadalupe. “Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.” 2018. Thesis, Brown University. Accessed January 19, 2021. https://repository.library.brown.edu/studio/item/bdr:792836/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paredes, Maria Guadalupe. “Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine.” 2018. Web. 19 Jan 2021.

Vancouver:

Paredes MG. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Jan 19]. Available from: https://repository.library.brown.edu/studio/item/bdr:792836/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paredes MG. Utilizing geometric cues for hiPSC-cardiomyocytes maturation on 2D patterned surfaces for regenerative medicine. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792836/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

2. Kolijn, K. The origin and future of hematopoietic stem cells.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/238606

► Hematopoietic stem cell (HSCs) therapy in the form of bone marrow transplantations has been used successfully in the clinic for over 40 years and continues… (more)

Subjects/Keywords: Hematopoietic stem cells; embryonic stem cells; induced pluripotent stem cells; ontogeny

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APA (6^th Edition):

Kolijn, K. (2012). The origin and future of hematopoietic stem cells. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/238606

Chicago Manual of Style (16^th Edition):

Kolijn, K. “The origin and future of hematopoietic stem cells.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 19, 2021. http://dspace.library.uu.nl:8080/handle/1874/238606.

MLA Handbook (7^th Edition):

Kolijn, K. “The origin and future of hematopoietic stem cells.” 2012. Web. 19 Jan 2021.

Vancouver:

Kolijn K. The origin and future of hematopoietic stem cells. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 19]. Available from: http://dspace.library.uu.nl:8080/handle/1874/238606.

Council of Science Editors:

Kolijn K. The origin and future of hematopoietic stem cells. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/238606

Louisiana State University

3. Addison, Meredith Kathleen. Lentiviral transduction of epigenetically modified bovine adult stem cells.

Degree: MS, Animal Sciences, 2012, Louisiana State University

URL: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

► Bovine adipose-derived stem cells (ADS), a form of adult stem cells, are somatic cells that have similar characteristics of embryonic stem (ES) cells. Bovine ADS… (more)

▼ Bovine adipose-derived stem cells (ADS), a form of adult stem cells, are somatic cells that have similar characteristics of embryonic stem (ES) cells. Bovine ADS cells possess multipotent capabilities and have been found to express pluripotency genes associated with ES cells. The unique properties of ADS cells make them a desirable source for reprogramming experiments. The goal of reprogramming experiments is to transform somatic cells from a differentiated state to a pluripotent state. When somatic cells reprogram, there are certain epigenetic changes or modifications that must occur in order to successfully reprogram the nucleus. Epigenetic modifications will change the chromatin configuration without changing the DNA sequence. Somatic cells can be exposed to small molecules that may be able to reduce the chances of having incomplete chromatin modification. Two epigenetic modifying factors are a DNA methyltranferase inhibitor, zebularine (Zeb), and a histone deacetylase inhibitor, valproic acid (VPA). By inducing gene expression with the epigenetic modifiers, the cells may be stimulated to reprogram more efficiently than cells with lower gene expression. In the first experiment, three bovine ADS cell lines were treated with VPA or Zeb to observe the changes in expression levels of Oct4, Sox2, and Nanog (pluripotency-associated genes). The cells were treated for a period of 5, 7,10, or 14 days. VPA led to the highest increase of the pluripotency genes; however, both treatments may have produced a partial reprogramming. This partial reprogramming may result in the bovine ADS cells reaching complete pluripotency when combined with a reprogramming technique. In the second experiment, three bovine ADS cell lines were treated with VPA or Zeb for five days then followed with transduction using lentivirus. Oct4, Sox2, and Nanog were increased the highest when using epigenetic modifiers. Statistical differences for expression of the pluripotency-associated genes were found for cells treated with zebularine. While it was thought that viral transduction in combination with epigenetic modifiers would produce higher expression levels of the pluripotency-associated genes, this was not found to be true in this experiment.

Subjects/Keywords: bovine adult stem cells; induced pluripotent stem cells; stem cells

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APA (6^th Edition):

Addison, M. K. (2012). Lentiviral transduction of epigenetically modified bovine adult stem cells. (Masters Thesis). Louisiana State University. Retrieved from etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

Chicago Manual of Style (16^th Edition):

Addison, Meredith Kathleen. “Lentiviral transduction of epigenetically modified bovine adult stem cells.” 2012. Masters Thesis, Louisiana State University. Accessed January 19, 2021. etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024.

MLA Handbook (7^th Edition):

Addison, Meredith Kathleen. “Lentiviral transduction of epigenetically modified bovine adult stem cells.” 2012. Web. 19 Jan 2021.

Vancouver:

Addison MK. Lentiviral transduction of epigenetically modified bovine adult stem cells. [Internet] [Masters thesis]. Louisiana State University; 2012. [cited 2021 Jan 19]. Available from: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024.

Council of Science Editors:

Addison MK. Lentiviral transduction of epigenetically modified bovine adult stem cells. [Masters Thesis]. Louisiana State University; 2012. Available from: etd-07162012-103728 ; https://digitalcommons.lsu.edu/gradschool_theses/4024

Universiteit Utrecht

4. Wever, I. From fibroblast to neuron: The use of stem cells for Parkinson's disease.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/258934

► One of the characterizations of Parkinson’s disease (PD) is the motor symptoms caused by the loss of the dopaminergic neurons of the substantia nigra pars… (more)

Subjects/Keywords: Parkinson's disease; Pluripotent Stem cells; Embryonic Stem cells; induced Pluripotent Stem cells; Neural development

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APA (6^th Edition):

Wever, I. (2012). From fibroblast to neuron: The use of stem cells for Parkinson's disease. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/258934

Chicago Manual of Style (16^th Edition):

Wever, I. “From fibroblast to neuron: The use of stem cells for Parkinson's disease.” 2012. Masters Thesis, Universiteit Utrecht. Accessed January 19, 2021. http://dspace.library.uu.nl:8080/handle/1874/258934.

MLA Handbook (7^th Edition):

Wever, I. “From fibroblast to neuron: The use of stem cells for Parkinson's disease.” 2012. Web. 19 Jan 2021.

Vancouver:

Wever I. From fibroblast to neuron: The use of stem cells for Parkinson's disease. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Jan 19]. Available from: http://dspace.library.uu.nl:8080/handle/1874/258934.

Council of Science Editors:

Wever I. From fibroblast to neuron: The use of stem cells for Parkinson's disease. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/258934

University of Southern California

5. Pomeroy, Jordan Elliott. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.

Degree: PhD, Systems Biology and Disease, 2012, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

► Future use of pluripotent cells for regenerative medicine will require the derivation and characterization of new cell lines in clinical grade conditions. Removing potential sources… (more)

▼ Future use of pluripotent cells for regenerative medicine will require the derivation and characterization of new cell lines in clinical grade conditions. Removing potential sources of contamination, such as cell culture components sourced from animals, will aid the regulatory approval for future stem cell based therapeutics. In this dissertation, I describe the development of a xenobiotic-free cell culture system for the derivation and maintenance of human pluripotent cell lines. I have derived >40 hiPSC lines and one hESC line in the xeno-free cell culture conditions with maintenance of pluripotency charcacterized by morphology and immunocytochemistry marker expression for >50 passages and >30 passages respectively. The hESC line, USC-01 and several hiPSC lines derived for this study demonstrate highly similar gene expression patterns although slight differences are apparent. USC-01 and several hiPSC lines demonstrate the ability to differentiate into cells displaying characteristics of all three germ layers in an embryoid body differentiation assay. Further examination of the process of reprogramming somatic cells to a pluripotent state at both the RNA and protein expression levels indicates several genes/markers selective for hiPSCs achieving a pluripotent state most similar to the “gold-standard” of pluripotency possessed by hESCs. This study confirms the usefulness of the markers TRA-1-60, E-Cadherin and EpCAM for live-cell selection of the best hiPSC colonies and also demonstrates the usefulness of the marker GCTM-2. Expression analysis of colonies undergoing reprogramming also indicates that the genes FOXD3, CDH3, LCK, EDNRB, EPHA1, SOX2, and HAS3 are active in only a small subset of colonies 30 days after transfection of the piggyBac transposon reprogramming cassette. Since these genes are active in all hESC and most hiPSC positive control lines tested, confirmation of their activation could be used to select reprogramming hiPSC colonies most likely to achieve a pluripotent state similar to hESCs. Increased selection and derivation efficiencies of hiPSC lines demonstrating high fidelity to the hESC pluripotent state will streamline the generation of hiPSC lines for future testing as a replacement for hESCs in regenerative medicine. Advisors/Committee Members: Pera, Martin F. (Committee Chair), Ying, Qi-Long (Committee Member), Lu, Wange (Committee Member), Chuong, Cheng-Ming (Committee Member).

Subjects/Keywords: embryonic; stem; induced; pluripotent; cells; clinical; human

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APA (6^th Edition):

Pomeroy, J. E. (2012). Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

Chicago Manual of Style (16^th Edition):

Pomeroy, Jordan Elliott. “Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.” 2012. Doctoral Dissertation, University of Southern California. Accessed January 19, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847.

MLA Handbook (7^th Edition):

Pomeroy, Jordan Elliott. “Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions.” 2012. Web. 19 Jan 2021.

Vancouver:

Pomeroy JE. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. [Internet] [Doctoral dissertation]. University of Southern California; 2012. [cited 2021 Jan 19]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847.

Council of Science Editors:

Pomeroy JE. Derivation and characterization of human embryonic stem (hES) cells and human induced pluripotent stem (hiPS) cells in clinical grade conditions. [Doctoral Dissertation]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/20555/rec/1847

California State University – Sacramento

6. Roenspie, Sean P. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.

Degree: MA, Biological Science (Stem Cell, 2011, California State University – Sacramento

URL: http://hdl.handle.net/10211.9/1093

► Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult onset neurodegenerative disorder affecting carriers of premutation expansions (55-200 CGG repeats) of the fragile X mental retardation… (more)

▼ Fragile X-associated tremor/ataxia syndrome (FXTAS) is an adult onset neurodegenerative disorder affecting carriers of premutation expansions (55-200 CGG repeats) of the fragile X mental retardation 1 (FMR1) gene. The clinical features of FXTAS, as well as various forms of clinical involvement in carriers without FXTAS, are thought to arise through a direct toxic gain-of-function of the FMR1 mRNA containing the expanded CGG repeat. A singular difficulty in the study of cellular regulation in FXTAS, as with most other neurogenetic disorders, is background genetic variation between/among cases and controls. To circumvent this problem, a novel approach was taken whereby single allele sub-clones were derived from a single individual who was profoundly mosaic for CGG repeat size, with CGG repeats in individual alleles ranging from the normal range (<50 CGG repeats) to well into the full mutation range (>700 CGG repeats). Sub-clones with expanded CGG repeat alleles, and those with CGG repeats in the normal range, thus represent background isogenic case-control comparison groups, since all clones derive from a single individual. In order to generate clonal fibroblast cell lines from the CGG repeat size mosaic, it was necessary to first develop a protocol that would successfully isolate single cells and allow for their efficient expansion. Once the protocol was established, a PCR-based method was used to genotype with respect to CGG repeat size each of the clonal cell lines. Through this process, fifteen clonal lines have been established to date, with representatives from normal (<55 CGG repeats), premutation (55-200 repeats) and full mutation (>200 repeats) ranges. These clonal lines are currently being characterized with respect to FMR1 gene expression (FMR1 mRNA and protein levels). The current clonal library forms the basis for our effort to reprogram the fibroblast cell lines into induced Pluripotent Stem Cells (iPSCs) with the intention of further differentiating the cells into neural progenitor cells, and subsequently, functional neurons. This research project was conducted in the department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, in the laboratory of Dr. Paul J Hagerman. Advisors/Committee Members: Peavy, Thomas R..

Subjects/Keywords: FXTAS; Neurodegenerative diseases; Induced pluripotent stem cells

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APA (6^th Edition):

Roenspie, S. P. (2011). An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. (Masters Thesis). California State University – Sacramento. Retrieved from http://hdl.handle.net/10211.9/1093

Chicago Manual of Style (16^th Edition):

Roenspie, Sean P. “An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.” 2011. Masters Thesis, California State University – Sacramento. Accessed January 19, 2021. http://hdl.handle.net/10211.9/1093.

MLA Handbook (7^th Edition):

Roenspie, Sean P. “An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles.” 2011. Web. 19 Jan 2021.

Vancouver:

Roenspie SP. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. [Internet] [Masters thesis]. California State University – Sacramento; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10211.9/1093.

Council of Science Editors:

Roenspie SP. An isogenic case-control study in which clonal cell lines are generated from a fibroblast population derived from an individual with a complex mosaic distribution of FMR1 alleles. [Masters Thesis]. California State University – Sacramento; 2011. Available from: http://hdl.handle.net/10211.9/1093

Laurentian University

7. Dénommé, Ginny Michelle. Induced pluripotent stem cells: where are we today? .

Degree: 2015, Laurentian University

URL: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

► For many years, scientists have been trying to elucidate the molecular mechanisms that convey pluripotency and self-renewal to stem cells. With this valuable knowledge, they… (more)

Subjects/Keywords: stem cells; somatic cells; induced pluripotent stem cells; self-renewal

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APA (6^th Edition):

Dénommé, G. M. (2015). Induced pluripotent stem cells: where are we today? . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dénommé, Ginny Michelle. “Induced pluripotent stem cells: where are we today? .” 2015. Thesis, Laurentian University. Accessed January 19, 2021. https://zone.biblio.laurentian.ca/dspace/handle/10219/2491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dénommé, Ginny Michelle. “Induced pluripotent stem cells: where are we today? .” 2015. Web. 19 Jan 2021.

Vancouver:

Dénommé GM. Induced pluripotent stem cells: where are we today? . [Internet] [Thesis]. Laurentian University; 2015. [cited 2021 Jan 19]. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dénommé GM. Induced pluripotent stem cells: where are we today? . [Thesis]. Laurentian University; 2015. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2491

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

8. Avery, John Welch. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.

Degree: 2018, University of Georgia

URL: http://hdl.handle.net/10724/37263

► Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling… (more)

▼ Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling the development of embryonic and adult tissues. In this work, we attempted a patient-specific disease in a dish model of the craniofacial disorder Treacher Collins Syndrome (TCS) and the directed differentiation of brown adipocytes from hPSCs. The TCS model was unable to replicate the expected phenotype of reduced neural crest cell numbers or migratory potential. This shortcoming has been putatively attributed to reducing and high antioxidant conditions of the culture medium. Modulating brown adipocyte activity in humans has become an attractive therapeutic target for obesity and related metabolic disorders such as type 2 diabetes. Murine models suggest the brown adipose organ is capable of significant changes in body mass and circulating levels of insulin and glucose. Methods to interrogate human brown adipocyte functionality have been based on adult primary cells or overexpression systems. Here we report a robust and efficient chemically-defined method to differentiate human pluripotent stem cells into brown adipocytes through a developmentally appropriate paraxial mesoderm intermediate. These adipocytes display characteristics of mature brown adipocytes including marker expression, responsiveness to stimulation, enhanced metabolic activity, thermogenic capacity, lipolysis, and utilization of fatty acids. These cells are capable of being maintained in culture for several weeks, are amenable to passage, and upon transplantation in mice can give rise to adipose depots that maintain both morphological and functional properties. We propose that these cells are appropriate for modeling brown adipocyte development in vivo, elucidating basic biological cell fate decisions and branch points and as a platform for high-throughput drug screening to identify putative therapeutic targets to combat obesity and related disorders.

Subjects/Keywords: Brown adipocytes; human pluripotent stem cells, induced pluripotent stem cells, neural crest cells, paraxial mesoderm

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APA (6^th Edition):

Avery, J. W. (2018). Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/37263

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Thesis, University of Georgia. Accessed January 19, 2021. http://hdl.handle.net/10724/37263.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Web. 19 Jan 2021.

Vancouver:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Internet] [Thesis]. University of Georgia; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10724/37263.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Thesis]. University of Georgia; 2018. Available from: http://hdl.handle.net/10724/37263

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

9. Avery, John Welch. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.

Degree: 2018, University of Georgia

URL: http://hdl.handle.net/10724/37106

► Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling… (more)

▼ Human pluripotent stem cells (hPSCs) are powerful tools to aid in the interrogation of the mechanisms of pathology of developmental disorders as well as modeling the development of embryonic and adult tissues. In this work, we attempted a patient-specific disease in a dish model of the craniofacial disorder Treacher Collins Syndrome (TCS) and the directed differentiation of brown adipocytes from hPSCs. The TCS model was unable to replicate the expected phenotype of reduced neural crest cell numbers or migratory potential. This shortcoming has been putatively attributed to reducing and high antioxidant conditions of the culture medium. Modulating brown adipocyte activity in humans has become an attractive therapeutic target for obesity and related metabolic disorders such as type 2 diabetes. Murine models suggest the brown adipose organ is capable of significant changes in body mass and circulating levels of insulin and glucose. Methods to interrogate human brown adipocyte functionality have been based on adult primary cells or overexpression systems. Here we report a robust and efficient chemically-defined method to differentiate human pluripotent stem cells into brown adipocytes through a developmentally appropriate paraxial mesoderm intermediate. These adipocytes display characteristics of mature brown adipocytes including marker expression, responsiveness to stimulation, enhanced metabolic activity, thermogenic capacity, lipolysis, and utilization of fatty acids. These cells are capable of being maintained in culture for several weeks, are amenable to passage, and upon transplantation in mice can give rise to adipose depots that maintain both morphological and functional properties. We propose that these cells are appropriate for modeling brown adipocyte development in vivo, elucidating basic biological cell fate decisions and branch points and as a platform for high-throughput drug screening to identify putative therapeutic targets to combat obesity and related disorders.

Subjects/Keywords: Brown adipocytes; human pluripotent stem cells, induced pluripotent stem cells, neural crest cells, paraxial mesoderm

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APA (6^th Edition):

Avery, J. W. (2018). Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Thesis, University of Georgia. Accessed January 19, 2021. http://hdl.handle.net/10724/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Avery, John Welch. “Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells.” 2018. Web. 19 Jan 2021.

Vancouver:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Internet] [Thesis]. University of Georgia; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10724/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Avery JW. Modeling treacher collins syndrome and directed differentiation of brown adipocytes from human pluripotent stem cells. [Thesis]. University of Georgia; 2018. Available from: http://hdl.handle.net/10724/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

10. Boer, A.S. de. iPS cell reprogramming.

Degree: 2009, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/35214

► Recently, pluripotent cells that are not derived from an embryo have been generated. These so called induced pluripotent stem cells (iPS cells) are pluripotent stem… (more)

Subjects/Keywords: Geneeskunde; embryonic stem cells, induced pluripotent stem cells, reprogramming, pluripotency, epigenetics

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APA (6^th Edition):

Boer, A. S. d. (2009). iPS cell reprogramming. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/35214

Chicago Manual of Style (16^th Edition):

Boer, A S de. “iPS cell reprogramming.” 2009. Masters Thesis, Universiteit Utrecht. Accessed January 19, 2021. http://dspace.library.uu.nl:8080/handle/1874/35214.

MLA Handbook (7^th Edition):

Boer, A S de. “iPS cell reprogramming.” 2009. Web. 19 Jan 2021.

Vancouver:

Boer ASd. iPS cell reprogramming. [Internet] [Masters thesis]. Universiteit Utrecht; 2009. [cited 2021 Jan 19]. Available from: http://dspace.library.uu.nl:8080/handle/1874/35214.

Council of Science Editors:

Boer ASd. iPS cell reprogramming. [Masters Thesis]. Universiteit Utrecht; 2009. Available from: http://dspace.library.uu.nl:8080/handle/1874/35214

11. 山添, 知宏. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.

Degree: 博士（医学）, 2015, Hamamatsu University School of Medicine / 浜松医科大学

URL: http://hdl.handle.net/10271/2879

►

Abstract. Although neural and mesenchymal stem cells have been well-known to have a strong glioma tropism, this activity in induced pluripotent stem cells (iPSCs) has… (more)

Subjects/Keywords: induced pluripotent stem cells; neural stem cells; glioma; migration; gene therapy

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APA (6^th Edition):

山添, �. (2015). Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. (Thesis). Hamamatsu University School of Medicine / 浜松医科大学. Retrieved from http://hdl.handle.net/10271/2879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

山添, 知宏. “Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.” 2015. Thesis, Hamamatsu University School of Medicine / 浜松医科大学. Accessed January 19, 2021. http://hdl.handle.net/10271/2879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

山添, 知宏. “Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する.” 2015. Web. 19 Jan 2021.

Vancouver:

山添 �. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. [Internet] [Thesis]. Hamamatsu University School of Medicine / 浜松医科大学; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10271/2879.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

山添 �. Potent tumor tropism of induced pluripotent stem cells and induced pluripotent stem cell-derived neural stem cells in the mouse intracerebral glioma model : iPS細胞およびiPS細胞由来神経幹細胞はマウス脳内グリオーマモデルにおいて腫瘍への活発な遊走能を有する. [Thesis]. Hamamatsu University School of Medicine / 浜松医科大学; 2015. Available from: http://hdl.handle.net/10271/2879

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

12. Schnabel, Lauren. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.

Degree: PhD, Veterinary Medicine, 2013, Cornell University

URL: http://hdl.handle.net/1813/34305

► Advancement of stem cell therapy is dependent upon the practicality, safety, and efficacy of the cells being evaluated for clinical application. Over the past decade,… (more)

▼ Advancement of stem cell therapy is dependent upon the practicality, safety, and efficacy of the cells being evaluated for clinical application. Over the past decade, the need for banked stem cells which are readily available for use at the time of a patient's diagnosis has become apparent. The overall goal of this dissertation research was to compare induced pluripotent stem cells (iPSCs) to bone marrow-derived mesenchymal stem cells (MSCs), first in terms of their ability to be generated from genetically diverse individuals, and then in terms of their immunogenic and immunomodulatory properties for potential allogeneic use. It has previously been demonstrated in mice that genetic background affects the proliferation and differentiation rates of MSCs. The purpose of our first study was to determine if genetic background affects the efficiency of generating iPSCs from mice. Results of this study confirmed that genetic background does affect both the efficiency of generating iPSCs during the early stages of reprogramming as well as the pluripotent stability of the iPSCs during later stages of reprogramming. The results also confirmed the need to understand the immunogenic and immunomodulatory properties of these cells for potential allogeneic application given that it may not be feasible to generate iPSCs from all individuals or to wait for the time that it takes to generate iPSCs and then screen them for safety and efficacy. The purpose of our second study, therefore, was to evaluate the in vitro immunogenic and immunomodulatory properties of murine iPSCs compared to MSCs using modified mixed leukocyte reactions. Our comparisons revealed that iPSCs generated through both lentiviral and piggyBac reprogramming methods had similar immunogenic properties as MSCs, and more potent immunomodulatory effects than MSCs. This information is critical when considering the use of iPSCs in the place of MSCs for both regenerative medicine and transplant medicine. Further studies must be performed, however, in order to determine if iPSCs retain their immunogenic and immunomodulatory properties upon differentiation into specific cell or tissue types. With this knowledge, we then shifted the focus of our third study to the horse, which is a valuable model for the human immune response. The purposes of this study were to immunophenotype MSCs from horses of known MHC haplotype and to compare the immunogenicity of MSCs with differing immunophenotypes, particularly in regards to MHC class II expression, through modified mixed leukocyte reactions. Results of this study demonstrated for the first time the extreme heterogeneity that exists in MHC class II expression by equine MSCs and that MHC class II positive equine MSCs are capable of inciting an immune response in vitro. This knowledge is critical for the treatment of our equine patients as well as for studies using the horse as an animal model for human diseases. Future experiments to determine if we can modulate this MHC class II expression in culture will be of great interest prior to… Advisors/Committee Members: Fortier, Lisa Ann (chair), Flaminio, Maria Julia Bevilaqua Felippe (committee member), Schimenti, John C. (committee member), Gilbert, Robert Owen (committee member), Nagy, Andras Bartos (committee member), Roberson, Mark Stephen (committee member).

Subjects/Keywords: Induced pluripotent stem cells (iPSCs); Mesenhcymal stem cells (MSCs); Immunonology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schnabel, L. (2013). Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/34305

Chicago Manual of Style (16^th Edition):

Schnabel, Lauren. “Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.” 2013. Doctoral Dissertation, Cornell University. Accessed January 19, 2021. http://hdl.handle.net/1813/34305.

MLA Handbook (7^th Edition):

Schnabel, Lauren. “Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells.” 2013. Web. 19 Jan 2021.

Vancouver:

Schnabel L. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1813/34305.

Council of Science Editors:

Schnabel L. Immunogenic And Immunomodulatory Properties Of Induced Pluripotent Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34305

Uppsala University

13. Bartish, Margarita. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.

Degree: Biology Education Centre, 2012, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

► The derivation of pluripotent stem cells (now termed induced pluripotent stem cells, iPSC) from mature somatic cells was a finding of seminal importance to… (more)

Subjects/Keywords: induced pluripotent stem cells; stem cells; disease modeling; cellular reprogramming

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bartish, M. (2012). Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bartish, Margarita. “Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.” 2012. Thesis, Uppsala University. Accessed January 19, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bartish, Margarita. “Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome.” 2012. Web. 19 Jan 2021.

Vancouver:

Bartish M. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. [Internet] [Thesis]. Uppsala University; 2012. [cited 2021 Jan 19]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bartish M. Establishing iPSCs as a method to model neurodevelopment in Down’s syndrome. [Thesis]. Uppsala University; 2012. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-182353

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

14. GUNEWARDENE, NILIKSHA. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.

Degree: 2014, University of Melbourne

URL: http://hdl.handle.net/11343/43098

► In mammals, overexposure to loud noise, ototoxic medication or even ageing can incur irreversible damage to the sensory hair cells and spiral ganglion neurons (SGNs)… (more)

▼ In mammals, overexposure to loud noise, ototoxic medication or even ageing can incur irreversible damage to the sensory hair cells and spiral ganglion neurons (SGNs) resulting in sensorineural hearing loss (SNHL). Currently, the cochlear implant is the only available treatment for SNHL, but its functionality is dependent on a healthy complement of SGNs. Therefore in cases of severe SNHL, where the numbers of SGNs are significantly depleted, the efficacy of this neural prosthesis may be compromised. Using stem cells to replace damaged SGNs is an emerging therapeutic strategy for deafness. Whilst previous studies have explored the potential for several stem cell types, particularly human embryonic stem cells (hESCs) to replace SGNs, it will eventually be important that transplanted cells are from an autologous source. This thesis therefore aims to explore the potential of human induced pluripotent stem cells (hiPSCs) for SGN replacement. These cells offer the option of transplanting SGNs generated from a patient’s own cells to potentially restore hearing function and/or improve the efficiency of the cochlear implant. To investigate the potential of hiPSCs, it is first necessary to assess their potential to differentiate into a SGN lineage. In the first study of the thesis, an established neural induction protocol was used to differentiate two hiPSC lines (iPS1 and iPS2) and one human embryonic stem cell line (hESC, H9) towards a neurosensory lineage in vitro. Immunocytochemistry and qRT-PCR were used to analyse the expression of key markers involved in SGN development, at defined time points of differentiation. The hiPSC- and hESC-derived neurosensory progenitors expressed the dorsal hindbrain and otic placodal markers (PAX7 and PAX2), pro-neurosensory marker (SOX2), ganglion neuronal markers (NEUROD1, BRN3A, ISLET1, ßIII-tubulin, Neurofilament kDa 160) and sensory SGN markers (GATA3 and VGLUT1) over the time course examined. The hiPSC-and hESC-derived neurosensory progenitors had the highest expression levels of the sensory neural markers at 35 days in vitro. Whilst all cell lines analysed produced neurosensory-like progenitors, variabilities in the levels of marker expression were observed between hiPSC lines and within samples of the same cell line, when compared to the hESC controls. Thereby, suggesting that hiPSCs have a more variable differentiation potential compared to the hESCs. The functionality of the hiPSC-derived neurons was next assessed using patch clamp electrophysiology and in vitro co-culture assays. It was found that the cells were capable of firing action potentials in response to depolarisation and exhibited a phasic profile of activity, thus indicating that the neurons were physiologically active. Following co-culture of cochlear explants or denervated explants with hiPSC- and hESC-derived neurons, their neural processes were observed to make direct contact and form extensive synaptic connections with inner and outer hair cells in vitro. However the hiPSC-derived neurons were…

Subjects/Keywords: stem cells; spiral ganglion; cochlea; induced pluripotent stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

GUNEWARDENE, N. (2014). The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/43098

Chicago Manual of Style (16^th Edition):

GUNEWARDENE, NILIKSHA. “The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.” 2014. Doctoral Dissertation, University of Melbourne. Accessed January 19, 2021. http://hdl.handle.net/11343/43098.

MLA Handbook (7^th Edition):

GUNEWARDENE, NILIKSHA. “The potential of induced pluripotent stem cells for spiral ganglion neuron replacement.” 2014. Web. 19 Jan 2021.

Vancouver:

GUNEWARDENE N. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. [Internet] [Doctoral dissertation]. University of Melbourne; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11343/43098.

Council of Science Editors:

GUNEWARDENE N. The potential of induced pluripotent stem cells for spiral ganglion neuron replacement. [Doctoral Dissertation]. University of Melbourne; 2014. Available from: http://hdl.handle.net/11343/43098

UCLA

15. Chan, Ngam Fung David. Modeling Early Human Development with Human Pluripotent Stem Cells.

Degree: Molec, Cell, & Dev Biology, 2012, UCLA

URL: http://www.escholarship.org/uc/item/6wm0k0pd

► Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) are pluripotent stem cells (hPSCs) that have the capacity to differentiate into a panoply… (more)

▼ Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) are pluripotent stem cells (hPSCs) that have the capacity to differentiate into a panoply of various cell types and to serve as a model for human development. Nevertheless, it is unknown whether in vitro development of hPSCs mirrors in vivo human development. We performed comprehensive transcriptome profiling to compare hPSC-derived progeny with tissue-derived counterparts. While hESC and hiPSC make nearly identical progeny for the neural, hepatic and mesenchymal lineages, these hPSC-derived progeny maintained, regardless of the cell lineage, the expression of genes normally associated with early development, including LIN28A, LIN28B and DPPA4. These data and expression patterns in early human fetal tissue suggested the hPSC-derivatives were reflective of very early human development. The LIN28/let-7 pathway has been known to regulate development. We hypothesize that high Lin28 and low level let-7 activities may be inhibiting the developmental maturity in hPSC-derivatives. In addition, such a LIN28/let-7 expression pattern is also thought to contribute to tumor aggressiveness. We have established a human cell-based system to screen for small molecules that could modulate LIN28/let-7 activity. This screening could potentially unveil powerful small molecules that could modulate developmental maturity and therapeutically combat cancer. Previous reports and our own transcriptional profiling indicate that hPSCs are suitable for modeling early human development. Epithelial to mesenchymal transitions (EMTs) are thought to be essential to generate diversity of tissues during early fetal development, but these events are impossible to study at the molecular level in vivo in humans. The first EMT event in human development occurs just prior to generation of the primitive streak. Because hPSCs are thought to most closely resemble cells found in epiblast stage embryos prior to formation of the primitive streak, we sought to model this first human EMT in vitro with hPSCs. We have demonstrated the EMT progression in hPSCs with embryoid bodies. We also identified PTK7 as a marker of this EMT population, which was used to purify these cells for identification of novel markers of human germ layer development.

Subjects/Keywords: Developmental biology; Molecular biology; development; differentiation; embryonic stem cells; induced pluripotent stem cells; pluripotent stem cells; stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chan, N. F. D. (2012). Modeling Early Human Development with Human Pluripotent Stem Cells. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/6wm0k0pd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chan, Ngam Fung David. “Modeling Early Human Development with Human Pluripotent Stem Cells.” 2012. Thesis, UCLA. Accessed January 19, 2021. http://www.escholarship.org/uc/item/6wm0k0pd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chan, Ngam Fung David. “Modeling Early Human Development with Human Pluripotent Stem Cells.” 2012. Web. 19 Jan 2021.

Vancouver:

Chan NFD. Modeling Early Human Development with Human Pluripotent Stem Cells. [Internet] [Thesis]. UCLA; 2012. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/6wm0k0pd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chan NFD. Modeling Early Human Development with Human Pluripotent Stem Cells. [Thesis]. UCLA; 2012. Available from: http://www.escholarship.org/uc/item/6wm0k0pd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

16. Brubaker, Chelsee. Stem cells: an overview of therapeutic approaches.

Degree: MS, Biomedical Research Technologies, 2017, Boston University

URL: http://hdl.handle.net/2144/26618

► The complexity of life exhibited in humans and other living creatures has drawn many to investigate the principles associated with organismal growth and development. A… (more)

Subjects/Keywords: Biology; Clinical medicine; Embryonic stem cells; Genome engineering; Induced pluripotent stem cells; Reprogramming; Stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brubaker, C. (2017). Stem cells: an overview of therapeutic approaches. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/26618

Chicago Manual of Style (16^th Edition):

Brubaker, Chelsee. “Stem cells: an overview of therapeutic approaches.” 2017. Masters Thesis, Boston University. Accessed January 19, 2021. http://hdl.handle.net/2144/26618.

MLA Handbook (7^th Edition):

Brubaker, Chelsee. “Stem cells: an overview of therapeutic approaches.” 2017. Web. 19 Jan 2021.

Vancouver:

Brubaker C. Stem cells: an overview of therapeutic approaches. [Internet] [Masters thesis]. Boston University; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2144/26618.

Council of Science Editors:

Brubaker C. Stem cells: an overview of therapeutic approaches. [Masters Thesis]. Boston University; 2017. Available from: http://hdl.handle.net/2144/26618

University of Edinburgh

17. Sartori, Chiara. Generation of ovine induced pluripotent stem cells.

Degree: PhD, 2012, University of Edinburgh

URL: http://hdl.handle.net/1842/6491

► Embryonic stem cells (ESCs) are pluripotent cells derived from the early embryo and are able to differentiate into cells belonging to the three germ layers.… (more)

Subjects/Keywords: 636.089; ovine iPS cells; induced pluripotent stem cells; iPSCs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sartori, C. (2012). Generation of ovine induced pluripotent stem cells. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/6491

Chicago Manual of Style (16^th Edition):

Sartori, Chiara. “Generation of ovine induced pluripotent stem cells.” 2012. Doctoral Dissertation, University of Edinburgh. Accessed January 19, 2021. http://hdl.handle.net/1842/6491.

MLA Handbook (7^th Edition):

Sartori, Chiara. “Generation of ovine induced pluripotent stem cells.” 2012. Web. 19 Jan 2021.

Vancouver:

Sartori C. Generation of ovine induced pluripotent stem cells. [Internet] [Doctoral dissertation]. University of Edinburgh; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1842/6491.

Council of Science Editors:

Sartori C. Generation of ovine induced pluripotent stem cells. [Doctoral Dissertation]. University of Edinburgh; 2012. Available from: http://hdl.handle.net/1842/6491

18. Avijgan, Mahtab. Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage .

Degree: Chalmers tekniska högskola / Institutionen för fysik, 2020, Chalmers University of Technology

URL: http://hdl.handle.net/20.500.12380/301119

► Diabetes is a chronic disease characterized by increased blood sugar because of dysfunctional glucose homeostasis by the beta cells in the pancreas. Functional beta cells… (more)

Subjects/Keywords: induced pluripotent stem cells; MafA; beta cells differentiation; pancreas; FACS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Avijgan, M. (2020). Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage . (Thesis). Chalmers University of Technology. Retrieved from http://hdl.handle.net/20.500.12380/301119

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Avijgan, Mahtab. “Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage .” 2020. Thesis, Chalmers University of Technology. Accessed January 19, 2021. http://hdl.handle.net/20.500.12380/301119.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Avijgan, Mahtab. “Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage .” 2020. Web. 19 Jan 2021.

Vancouver:

Avijgan M. Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage . [Internet] [Thesis]. Chalmers University of Technology; 2020. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/20.500.12380/301119.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Avijgan M. Differentiation and characterization of MafA-GFP reporter iPS cells into beta cell lineage . [Thesis]. Chalmers University of Technology; 2020. Available from: http://hdl.handle.net/20.500.12380/301119

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oxford

19. Sachamitr, Supatra. Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy.

Degree: PhD, 2015, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:89315b6b-a8cd-4a6f-8c43-3506d8dd1725 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712057

► Immunotherapy traditionally made use of biological agents such as cytokines and monoclonal antibodies. Such first generation therapies lack antigen specificity and fail to induce immunological… (more)

▼ Immunotherapy traditionally made use of biological agents such as cytokines and monoclonal antibodies. Such first generation therapies lack antigen specificity and fail to induce immunological memory, suggesting that cell therapies may provide the next generation of treatments that are more discerning in their mode of action. Nevertheless, difficulties in obtaining sufficient immunologically-relevant cell types from patients has limited their success. Given that induced pluripotent stem cells (iPSC) may be generated from patients, we have investigated the feasibility of deriving two cell types whose availability is restricted in vivo: regulatory T cells (T_regs) and CD141⁺ cross-presenting dendritic cells (DCs). We describe the optimization of protocols for differentiation and purification of CD141⁺ DCs, focussing on their utility as a therapeutic vaccine for HIV-1. We investigate their phenotype, chemotactic capacity, phagocytic ability and propensity to harbour infectious virus. We also assess their immunostimulatory capacity and ability to cross-present exogenous antigen to MHC class I-restricted T cells. Our findings led us to speculate that iPSC-derived DCs (iPDCs) possess fetal phenotype, which is characterised by excessive secretion of IL-10 and failure to secrete IL-12, under all but the most stringent conditions. We hypothesised that constitutive secretion of IL-10 may be responsible for maintaining the fetal phenotype, a hypothesis we tested by developing an appropriate mouse model. iPSCs were derived from WT and IL-10^-/- mice and were shown to differentiate into iPDCs which recapitulate the fetal phenotype observed among human cells. However, loss of the endogenous Il-10 gene failed to restore full immunogenicity and IL-12 secretion. Finally, we developed protocols for differentiation of FoxP3+ T_regs from iPSCs, a feat that has not previously been achieved. These findings pave the way for the differentiation of T_regs from iPSCs reprogrammed from antigen-specific pathogenic T cells, thereby creating a source of T_regs with matched specificity for therapeutic intervention.

Subjects/Keywords: 616.07; Stem cells; Dendritic cells; HIV infections – Immunological aspects; Immunotherapy; HIV; induced pluripotent stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sachamitr, S. (2015). Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:89315b6b-a8cd-4a6f-8c43-3506d8dd1725 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712057

Chicago Manual of Style (16^th Edition):

Sachamitr, Supatra. “Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy.” 2015. Doctoral Dissertation, University of Oxford. Accessed January 19, 2021. http://ora.ox.ac.uk/objects/uuid:89315b6b-a8cd-4a6f-8c43-3506d8dd1725 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712057.

MLA Handbook (7^th Edition):

Sachamitr, Supatra. “Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy.” 2015. Web. 19 Jan 2021.

Vancouver:

Sachamitr S. Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy. [Internet] [Doctoral dissertation]. University of Oxford; 2015. [cited 2021 Jan 19]. Available from: http://ora.ox.ac.uk/objects/uuid:89315b6b-a8cd-4a6f-8c43-3506d8dd1725 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712057.

Council of Science Editors:

Sachamitr S. Exploiting the use of induced pluripotent stem cell derived immune cells for immunotherapy. [Doctoral Dissertation]. University of Oxford; 2015. Available from: http://ora.ox.ac.uk/objects/uuid:89315b6b-a8cd-4a6f-8c43-3506d8dd1725 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712057

University of Manchester

20. Vitillo, Loriana. Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells.

Degree: 2013, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:215044

► Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the… (more)

▼ Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the hESCs pluripotent niche and supports their maintenance, but the contribution to survival remains to be elucidated. Understanding the mechanism of survival is particularly crucial in hESCs, since it affects their expansion in cell culture and ultimately translation of research to the clinic. HESCs bind to FN mainly via α5β1- integrin, known to be upstream of important survival cascades in other cell types. However, it is not understood if and how FN/integrin binding supports those molecular pathways in the context of pluripotent hESCs. The aim of this work was to elucidate the survival cascade downstream of the FN/integrin interaction in hESCs. Initially, when hESCs were cultured on a non-integrin activating substrate they initiated an apoptotic response that also occurred when β1-integrin was selectively blocked with antibody, leading the cells to detach from FN. Integrin activation is generally transduced within cells via a complex adhesome of scaffold and kinase proteins, among which the focal adhesion kinase (FAK) plays a key role. Indeed, blocking β1-integrin resulted in dephosphorylation of endogenous FAK in hESCs. When FAK kinase activity was directly inhibited (with small molecule inhibitors), hESCs responded by detaching from FN and activating caspase-3, leading to an increase in apoptosis. Furthermore, flow cytometry analysis showed that the population of hESCs that underwent apoptosis still retained the pluripotency-associated marker NANOG. FAK is a convergent point between growth factor signaling and the PI3K/Akt pathway, with a well-reported role in the maintenance of hESCs. Consistently, FN activated both AKT and its target the ubiquitin ligase MDM2 at the protein levels, while pAkt was reduced after β1-integrin blocking and FAK inhibition. Cell imaging showed that MDM2, which regulates p53 degradation in the nucleus, displayed reduced nuclear localisation after FAK inhibition, opening the possibility for a change in the p53 balance in hESCs. In fact, p53 protein increases after FAK inhibition corresponding also to caspase activation. Further investigation explored if FAK-dependent pathways are also implicated in the maintenance of hESC pluripotency. Inhibition of FAK led the cells that survived apoptosis to lose stem cell morphology, decrease pluripotency-associated markers and change nuclear shape. Moreover, a small pool of FAK was found in the nucleus of hESCs cultured on FN, but decreased after FAK inhibition. FAK was also co- immunoprecipitated with NANOG protein in standard hESC culture while NANOG decreased after sustained FAK inhibition. This data suggests that nuclear roles of FAK could support, together with the cytoplasmic activation of the PI3K cascade, both survival and pluripotency pathways requiring further investigation. In conclusion, the original contribution of this work is to identify in FAK the downstream… Advisors/Committee Members: GILMORE, ANDREW AP, Kimber, Susan, Gilmore, Andrew.

Subjects/Keywords: Stem Cells; Pluripotent stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vitillo, L. (2013). Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:215044

Chicago Manual of Style (16^th Edition):

Vitillo, Loriana. “Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells.” 2013. Doctoral Dissertation, University of Manchester. Accessed January 19, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:215044.

MLA Handbook (7^th Edition):

Vitillo, Loriana. “Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells.” 2013. Web. 19 Jan 2021.

Vancouver:

Vitillo L. Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Jan 19]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:215044.

Council of Science Editors:

Vitillo L. Focal Adhesion Kinase Regulation of Human Embryonic Stem Cells. [Doctoral Dissertation]. University of Manchester; 2013. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:215044

University of Manchester

21. Vitillo, Loriana. Focal adhesion kinase regulation of human embryonic stem cells.

Degree: PhD, 2014, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764277

► Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the… (more)

▼ Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the hESCs pluripotent niche and supports their maintenance, but the contribution to survival remains to be elucidated. Understanding the mechanism of survival is particularly crucial in hESCs, since it affects their expansion in cell culture and ultimately translation of research to the clinic. HESCs bind to FN mainly via alpha5β1- integrin, known to be upstream of important survival cascades in other cell types. However, it is not understood if and how FN/integrin binding supports those molecular pathways in the context of pluripotent hESCs. The aim of this work was to elucidate the survival cascade downstream of the FN/integrin interaction in hESCs. Initially, when hESCs were cultured on a non-integrin activating substrate they initiated an apoptotic response that also occurred when β1-integrin was selectively blocked with antibody, leading the cells to detach from FN. Integrin activation is generally transduced within cells via a complex adhesome of scaffold and kinase proteins, among which the focal adhesion kinase (FAK) plays a key role. Indeed, blocking β1-integrin resulted in dephosphorylation of endogenous FAK in hESCs. When FAK kinase activity was directly inhibited (with small molecule inhibitors), hESCs responded by detaching from FN and activating caspase-3, leading to an increase in apoptosis. Furthermore, flow cytometry analysis showed that the population of hESCs that underwent apoptosis still retained the pluripotency-associated marker NANOG. FAK is a convergent point between growth factor signaling and the PI3K/Akt pathway, with a well-reported role in the maintenance of hESCs. Consistently, FN activated both AKT and its target the ubiquitin ligase MDM2 at the protein levels, while pAkt was reduced after β1-integrin blocking and FAK inhibition. Cell imaging showed that MDM2, which regulates p53 degradation in the nucleus, displayed reduced nuclear localisation after FAK inhibition, opening the possibility for a change in the p53 balance in hESCs. In fact, p53 protein increases after FAK inhibition corresponding also to caspase activation. Further investigation explored if FAK-dependent pathways are also implicated in the maintenance of hESC pluripotency. Inhibition of FAK led the cells that survived apoptosis to lose stem cell morphology, decrease pluripotency-associated markers and change nuclear shape. Moreover, a small pool of FAK was found in the nucleus of hESCs cultured on FN, but decreased after FAK inhibition. FAK was also co- immunoprecipitated with NANOG protein in standard hESC culture while NANOG decreased after sustained FAK inhibition. This data suggests that nuclear roles of FAK could support, together with the cytoplasmic activation of the PI3K cascade, both survival and pluripotency pathways requiring further investigation. In conclusion, the original contribution of this work is to identify in FAK the downstream…

Subjects/Keywords: Stem Cells; Pluripotent stem cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vitillo, L. (2014). Focal adhesion kinase regulation of human embryonic stem cells. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764277

Chicago Manual of Style (16^th Edition):

Vitillo, Loriana. “Focal adhesion kinase regulation of human embryonic stem cells.” 2014. Doctoral Dissertation, University of Manchester. Accessed January 19, 2021. https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764277.

MLA Handbook (7^th Edition):

Vitillo, Loriana. “Focal adhesion kinase regulation of human embryonic stem cells.” 2014. Web. 19 Jan 2021.

Vancouver:

Vitillo L. Focal adhesion kinase regulation of human embryonic stem cells. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Jan 19]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764277.

Council of Science Editors:

Vitillo L. Focal adhesion kinase regulation of human embryonic stem cells. [Doctoral Dissertation]. University of Manchester; 2014. Available from: https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764277

University of Toronto

22. Salewski, Ryan P. Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/68474

► Advancements in medical management for spinal cord injury (SCI) have resulted in greatly improved survival rates following this devastating event; although medical interventions to regenerate… (more)

▼ Advancements in medical management for spinal cord injury (SCI) have resulted in greatly improved survival rates following this devastating event; although medical interventions to regenerate the injured spinal cord and restore function remain limited. Neural stem cells (NSCs) from embryonic or fetal/adult tissues sources show considerable promise in regenerative strategies for traumatic SCI. However, there are limitations with their use related to availability, immunogenicity, immunological rejection and uncertainty of mechanism. To address these issues, our laboratory has investigated the use of NSCs derived from induced pluripotent stem (iPS) cells generated using a non-viral and mutation-free approach. NSCs were generated from pluripotent cells using a free-floating neurospheres methodology previously used by our lab. To delineate the mechanism of action, specifically the role of exogenous myelination, NSCs derived from wildtype (wt) and non-myelinating Shiverer (shi) iPS cell lines were used in a mouse thoracic SCI model with sub-acute intraspinal transplantation. Behavioral, histological and electrophysiological outcomes were analyzed to assess the safety and efficacy of this treatment. Variable neural propensity in the iPS cell lines were identified and overcome by agonizing the NOTCH pathway, yielding a clinically relevant population of NSCs for transplantation. The wt- and shi-iPS-NSCs were validated and shown to be equivalent except in myelination capacity. Both iPS-NSC lines were transplanted following thoracic SCI, but only the wt-iPS-NSC treatment resulted in a functional benefit. Wt-iPS-NSC transplantation resulted in significantly improved hindlimb locomotor function, histological outcomes and action potential amplitudes compared to the non-myelinating and cell-free controls. This supports remyelination as the key process by which NSC treatment provides a benefit following thoracic SCI. This work addresses a significant knowledge gap in the field of regenerative medicine for SCI. It demonstrated that pluripotent cells, specifically iPS cells, can be used to generate clinical relevant NSCs for application in SCI. Even though NSCs have been shown to provide a diverse range of functions in the spinal cord, remyelination is the predominate mechanism of recovery following thoracic SCI. This work will have significant impact in shaping how iPS-NSCs will be potentially translated to a clinical setting. Advisors/Committee Members: Michael, G Fehlings, Medical Science.

Subjects/Keywords: Embryonic Stem Cells; Induced Pluripotent Stem Cell; Myelination; Neural Stem Cell; Spinal Cord Injury; 0317

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salewski, R. P. (2014). Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/68474

Chicago Manual of Style (16^th Edition):

Salewski, Ryan P. “Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury.” 2014. Doctoral Dissertation, University of Toronto. Accessed January 19, 2021. http://hdl.handle.net/1807/68474.

MLA Handbook (7^th Edition):

Salewski, Ryan P. “Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury.” 2014. Web. 19 Jan 2021.

Vancouver:

Salewski RP. Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1807/68474.

Council of Science Editors:

Salewski RP. Definitive Neural Stem Cell Clonally Generated from Pluripotent Stem Cells Promote Recovery following Spinal Cord Injury. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/68474

23. 本勝, 希実子. Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells.

Degree: 博士(医学), 2015, University of Miyazaki / 宮崎大学

URL: http://hdl.handle.net/10458/5428

以下に掲載:The Journal of reproduction and development. 2015, Volume 61, No.1, p. 13-19, doi:10.1262/jrd.2014-098.

Subjects/Keywords: Embryonic stem cells (ESCs); Induced pluripotent stem cells (iPSCs); Naïve; Neural differentiation; Rabbit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

本勝, �. (2015). Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells. (Thesis). University of Miyazaki / 宮崎大学. Retrieved from http://hdl.handle.net/10458/5428

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

本勝, 希実子. “Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells.” 2015. Thesis, University of Miyazaki / 宮崎大学. Accessed January 19, 2021. http://hdl.handle.net/10458/5428.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

本勝, 希実子. “Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells.” 2015. Web. 19 Jan 2021.

Vancouver:

本勝 �. Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells. [Internet] [Thesis]. University of Miyazaki / 宮崎大学; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10458/5428.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

本勝 �. Naïve-like conversion enhances the difference in innate in vitro differentiation capacity between rabbit ES cells and iPS cells. [Thesis]. University of Miyazaki / 宮崎大学; 2015. Available from: http://hdl.handle.net/10458/5428

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

24. Hoeven, T. van den. Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/297927

► Induction of pluripotency is a process that reverses the phenotype of terminally differentiated cells. Somatic cells, induced by specific transcription factors, are reprogrammed to a… (more)

Subjects/Keywords: Stem Cells; Induced Pluripotent Stem Cells; iPS; pre-iPS; reprogramming; transcription factors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hoeven, T. v. d. (2014). Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/297927

Chicago Manual of Style (16^th Edition):

Hoeven, T van den. “Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 19, 2021. http://dspace.library.uu.nl:8080/handle/1874/297927.

MLA Handbook (7^th Edition):

Hoeven, T van den. “Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming.” 2014. Web. 19 Jan 2021.

Vancouver:

Hoeven Tvd. Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 19]. Available from: http://dspace.library.uu.nl:8080/handle/1874/297927.

Council of Science Editors:

Hoeven Tvd. Induced pluripotent stem cells: Induction of pluripotency and the molecular mechanism of partial reprogramming. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/297927

Royal Holloway, University of London

25. Boza Moran, Maria. Modelling SMA using induced pluripotent stem cells from a discordant affected family.

Degree: PhD, 2013, Royal Holloway, University of London

URL: https://pure.royalholloway.ac.uk/portal/en/publications/modelling-sma-using-induced-pluripotent-stem-cells-from-a-discordant-affected-family(22ab02dd-fe47-421f-98ac-830a2fd1624b).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589610

► Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease in which low levels of survival of motor neuron (SMN) protein lead to the degeneration… (more)

▼ Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease in which low levels of survival of motor neuron (SMN) protein lead to the degeneration of alpha motor neurons (MNs) in the spinal cord. The pathological mechanism of SMA is highly controversial and until recently it was not possible to obtain human MNs to study the disease. The development of induced pluripotent stem cell (iPSC) technology has made it possible to bypass this obstacle and iPSC-based models of SMA type I have already been validated by two separate groups. Encouraged by these pioneering findings I have produced and characterized iPSCs from several members of a discordant consanguineous family in which four haploidentical siblings share the same homozygous SMNl mutation, but nonetheless show different phenotypes of the disease. I have differentiated iPSC clones from three of the siblings and the carrier unaffected mother, as well as a control unaffected clone and a clone derived from a patient with SMA type I, into ISL1+/ChAT+ MNs. No obvious phenotypic difference was observed between the MN cultures of the siblings during the period of study, but cells from the SMA type I patient did show an impaired ability to form rosettes. The study of SMN and PLS3 levels during the differentiation from iPSCs to ChAT+ MNs showed a gradual decay of these proteins during MN development in all clones. Furthermore, SMN protein levels did not correlate with the pattern of mRNA expression, c suggesting the existence of post-transcriptional and/or post-translational regulation of full-length SMN (FL-SMN) transcripts and protein. The FL/.d7-SMN mRNA ratio and total SMN (tSMN) mRNA levels were found to be possible biomarkers to distinguish unaffected individuals from SMA patients and the severity of SMA pathology, respectively. PLS3 protein level was higher in the SMA type IV/asymptomatic sibling than in two of the type III SMA siblings, but it could not be confirmed as a modifier factor in the family. These results suggest that SMN levels are regulated during MN development, and that low levels may impair the generation of rosettes but not necessarily of MNs. SMN levels in MNs only show minor differences between patients, suggesting that there may be a threshold after which reduced levels of SMN within a narrow range become suddenly and increasingly detrimental unless modifier factors can compensate for the cellular function(s) lost. 3

Subjects/Keywords: 616.73; Spinal Muscular Atrophy; Stem Cells; Induced Pluripotent Stem Cells; Motor Neurons

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boza Moran, M. (2013). Modelling SMA using induced pluripotent stem cells from a discordant affected family. (Doctoral Dissertation). Royal Holloway, University of London. Retrieved from https://pure.royalholloway.ac.uk/portal/en/publications/modelling-sma-using-induced-pluripotent-stem-cells-from-a-discordant-affected-family(22ab02dd-fe47-421f-98ac-830a2fd1624b).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589610

Chicago Manual of Style (16^th Edition):

Boza Moran, Maria. “Modelling SMA using induced pluripotent stem cells from a discordant affected family.” 2013. Doctoral Dissertation, Royal Holloway, University of London. Accessed January 19, 2021. https://pure.royalholloway.ac.uk/portal/en/publications/modelling-sma-using-induced-pluripotent-stem-cells-from-a-discordant-affected-family(22ab02dd-fe47-421f-98ac-830a2fd1624b).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589610.

MLA Handbook (7^th Edition):

Boza Moran, Maria. “Modelling SMA using induced pluripotent stem cells from a discordant affected family.” 2013. Web. 19 Jan 2021.

Vancouver:

Boza Moran M. Modelling SMA using induced pluripotent stem cells from a discordant affected family. [Internet] [Doctoral dissertation]. Royal Holloway, University of London; 2013. [cited 2021 Jan 19]. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/modelling-sma-using-induced-pluripotent-stem-cells-from-a-discordant-affected-family(22ab02dd-fe47-421f-98ac-830a2fd1624b).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589610.

Council of Science Editors:

Boza Moran M. Modelling SMA using induced pluripotent stem cells from a discordant affected family. [Doctoral Dissertation]. Royal Holloway, University of London; 2013. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/modelling-sma-using-induced-pluripotent-stem-cells-from-a-discordant-affected-family(22ab02dd-fe47-421f-98ac-830a2fd1624b).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589610

University of Western Ontario

26. Tobias, Ian C. Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming.

Degree: 2019, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/6224

► Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) derived from large mammals reproduce few characteristics displayed by rodent or human counterparts. This is… (more)

▼ Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) derived from large mammals reproduce few characteristics displayed by rodent or human counterparts. This is complicated by the inherent plasticity of mammalian ESC/iPSC cultures that resemble a variety of developmental stages including the naïve and primed pluripotent states. Defining the extrinsic signals that modulate the developmental identity of canine ESC/iPSC (i.e. primed versus naïve) will improve knowledge integration with more sophisticated rodent and primate research. In this thesis, I sought to determine if manipulation of the culture environment can promote nuclear and metabolic reprogramming of canine cell lines towards a more primitive state of pluripotency. Using growth factors and kinase inhibitors to modulate pluripotency of canine ESCs (cESCs), I demonstrated that cESCs exhibit the plasticity to adopt multiple developmental identities. I observed that lineage-specific differentiation of cESCs is influenced by pluripotent state modulation, coincident with changes to colony architecture, transcriptional and epigenetic markers of developmental maturity. I found that primed- and naïve-like cESCs exhibit pluripotent-state specific mitochondrial structure and function, including differential reliance on glucose oxidation pathways for steady-state proliferation in vitro. Lastly, using comparative sequence analysis and biochemical assays, I observed that evolutionary differences in genomic CpG density at pluripotency-associated promoters correlate with functionally relevant cytosine modifications to the induction and maintenance of pluripotency. I showed the utility of physiological metabolites that regulate 5-methylcytosine oxidation in promoting cellular and transcriptional features associated with early nuclear reprogramming of canine fibroblasts (e.g. mesenchymal-to-epithelial transition). Taken together, my work establishes a binary cESC model of primed- and naïve-pluripotent states, providing insight into shared and divergent features of pluripotency progression and acquisition in placental mammals.

Subjects/Keywords: Canine; Embryonic Stem Cells; Induced Pluripotent Stem Cells; Naive Pluripotency; Mitochondria; DNA Methylation; Developmental Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tobias, I. C. (2019). Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tobias, Ian C. “Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming.” 2019. Thesis, University of Western Ontario. Accessed January 19, 2021. https://ir.lib.uwo.ca/etd/6224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tobias, Ian C. “Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming.” 2019. Web. 19 Jan 2021.

Vancouver:

Tobias IC. Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2021 Jan 19]. Available from: https://ir.lib.uwo.ca/etd/6224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tobias IC. Phenotypic and Metabolic Plasticity In Canine Cellular Reprogramming. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Kole, Denis. Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions.

Degree: PhD, 2014, Worcester Polytechnic Institute

URL: etd-042814-131416 ; https://digitalcommons.wpi.edu/etd-dissertations/207

► Current usage of human embryonic stem cells (hES) and induced pluripotent stem cells (iPS) in clinical therapies and personalized medicine are limited as a result… (more)

Subjects/Keywords: Induced pluripotent stem cells; Pluripotency; FGF2; Cellular reprogramming; Stem cells; Xenopus laevis egg extract

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kole, D. (2014). Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions. (Doctoral Dissertation). Worcester Polytechnic Institute. Retrieved from etd-042814-131416 ; https://digitalcommons.wpi.edu/etd-dissertations/207

Chicago Manual of Style (16^th Edition):

Kole, Denis. “Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions.” 2014. Doctoral Dissertation, Worcester Polytechnic Institute. Accessed January 19, 2021. etd-042814-131416 ; https://digitalcommons.wpi.edu/etd-dissertations/207.

MLA Handbook (7^th Edition):

Kole, Denis. “Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions.” 2014. Web. 19 Jan 2021.

Vancouver:

Kole D. Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions. [Internet] [Doctoral dissertation]. Worcester Polytechnic Institute; 2014. [cited 2021 Jan 19]. Available from: etd-042814-131416 ; https://digitalcommons.wpi.edu/etd-dissertations/207.

Council of Science Editors:

Kole D. Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions. [Doctoral Dissertation]. Worcester Polytechnic Institute; 2014. Available from: etd-042814-131416 ; https://digitalcommons.wpi.edu/etd-dissertations/207

University of Georgia

28. Smith, Keriayn Nicole. Roles for myc in self-renewal of pluripotent stem cells.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25949

► Myc is a critical factor for embryonic stem cell maintenance. It also enhances the reprogramming of fibroblasts by effecting widespread changes in gene expression, effectively… (more)

Subjects/Keywords: Embryonic stem cells; induced pluripotent stem cells; self-renewal; pluripotency; myc; Gata6; primitive endoderm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, K. N. (2014). Roles for myc in self-renewal of pluripotent stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25949

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Smith, Keriayn Nicole. “Roles for myc in self-renewal of pluripotent stem cells.” 2014. Thesis, University of Georgia. Accessed January 19, 2021. http://hdl.handle.net/10724/25949.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Smith, Keriayn Nicole. “Roles for myc in self-renewal of pluripotent stem cells.” 2014. Web. 19 Jan 2021.

Vancouver:

Smith KN. Roles for myc in self-renewal of pluripotent stem cells. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10724/25949.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Smith KN. Roles for myc in self-renewal of pluripotent stem cells. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25949

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Yoshikawa, Tatsuya. Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons.

Degree: 博士(医学), 2017, Mie University / 三重大学

URL: http://hdl.handle.net/10076/14576

本文 / Kyoto Univ, Inst Frontier Med Sci ; Mie Univ, Dept Neurosurg, Grad Sch Med ; Kyoto Univ, Grad Sch Med, Dept Neurosurg

10

Subjects/Keywords: induced pluripotent stem cells; valproic acid; zonisamide; estradiol; transplantation; dopaminergic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yoshikawa, T. (2017). Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons. (Thesis). Mie University / 三重大学. Retrieved from http://hdl.handle.net/10076/14576

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yoshikawa, Tatsuya. “Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons.” 2017. Thesis, Mie University / 三重大学. Accessed January 19, 2021. http://hdl.handle.net/10076/14576.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yoshikawa, Tatsuya. “Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons.” 2017. Web. 19 Jan 2021.

Vancouver:

Yoshikawa T. Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons. [Internet] [Thesis]. Mie University / 三重大学; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10076/14576.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yoshikawa T. Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons. [Thesis]. Mie University / 三重大学; 2017. Available from: http://hdl.handle.net/10076/14576

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

30. Burgess, Kyle. Peptide Hydrogels for Advanced 3D Cell Culture.

Degree: 2018, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:317703

► Stem cell technology is an invaluable tool in tissue engineering and regenerative medicine, with induced pluripotent stem cells (iPSCs) providing the means to design: autologous… (more)

▼ Stem cell technology is an invaluable tool in tissue engineering and regenerative medicine, with induced pluripotent stem cells (iPSCs) providing the means to design: autologous cell therapies, drug screening platforms and disease/ developmental models. However, the expansion of hiPSCs in vitro typically relies on either the presence of a layer of supportive feeder cells, or the use of ill-defined matrices. Moreover, following differentiation, iPSC-derivatives often lack the same phenotypic traits as their native counterparts. As such, the field of tissue engineering moves toward recapitulating the cell niche in vitro, through the design of biomaterials for 3D cell culture. With this in mind, the designs of new biomaterials are emerging all the time. Of particular focus are 'hydrogels'; a water-swollen network of polymeric fibres which entangle and/ or aggregate to form a self-supporting construct. These fibres can be made of natural and/ or synthetic polymers. Of great interest are peptide hydrogels. Peptide sequences can be designed to self-assemble into an array of larger macromolecular structures. One family of self-assembling peptide hydrogels (SAPHs) are based on an alternating sequence of hydrophilic/ hydrophobic amino acid residues which self-assemble to form beta-sheet nanofibres. Many variations of these SAPHs currently exist within the literature, e.g. EAK, RADA and KFE, and have already shown great promise in supporting the culture of many different cell lineages; a promising substrate for stem cell culture and tissue engineering applications. Importantly, when considering any biomaterial for 3D cell culture, it is vital to determine whether the abundance of material interferes with the method of analysis, either through interactions with the substrate of interest, or a component(s) of the technique. The work presented here is the first to outline the development of protocols for the following applications: 1) the extraction of RNA from cells encapsulated in SAPHs for downstream quantitative polymerase chain reaction (qPCR); and 2) the solubilisation of SAPHs and cell proteins for downstream western blot analysis. For RNA extraction, RNA was isolated from four peptide formulations - which vary in net charge - using either: 1) precipitation-based extraction, or 2) solid-state RNA binding to a silica membrane. The latter was more effective at removing contaminants but both methods resulted in a low yield - demonstrating a clear interference from the presence of SAP. However, enzymatic degradation of the hydrogel construct prior to RNA isolation significantly increased the amount of RNA recovered. On the other hand, for western blot analysis, the focus was on achieving complete solubilisation of both the self-assembling peptide (SAP) monomer and cell proteins following disruption of the hydrogel construct and the lysis of encapsulated cells. The use of urea - unlike a detergent-based buffer (Radioimmunoprecipitation assay buffer, aka RIPA) - in combination with multiple cycles of ultrasonication, was able to… Advisors/Committee Members: OCEANDY, DELVAC D, Saiani, Alberto, Oceandy, Delvac.

Subjects/Keywords: Peptide Hydrogels; Induced Pluripotent Stem Cells (iPSCs); 3D Culture

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burgess, K. (2018). Peptide Hydrogels for Advanced 3D Cell Culture. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:317703

Chicago Manual of Style (16^th Edition):

Burgess, Kyle. “Peptide Hydrogels for Advanced 3D Cell Culture.” 2018. Doctoral Dissertation, University of Manchester. Accessed January 19, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:317703.

MLA Handbook (7^th Edition):

Burgess, Kyle. “Peptide Hydrogels for Advanced 3D Cell Culture.” 2018. Web. 19 Jan 2021.

Vancouver:

Burgess K. Peptide Hydrogels for Advanced 3D Cell Culture. [Internet] [Doctoral dissertation]. University of Manchester; 2018. [cited 2021 Jan 19]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:317703.

Council of Science Editors:

Burgess K. Peptide Hydrogels for Advanced 3D Cell Culture. [Doctoral Dissertation]. University of Manchester; 2018. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:317703

Open Access Theses and Dissertations