subject:(immobilization)

University of Illinois – Chicago

1. Hill, Brittaney. Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/22043

► Objective: To determine if caregiver race/ethnicity impacts their willingness to accept passive immobilization and to determine if a written and pictorial description of the technique… (more)

Subjects/Keywords: Passive Immobilization

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APA (6^th Edition):

Hill, B. (2017). Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/22043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hill, Brittaney. “Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.” 2017. Thesis, University of Illinois – Chicago. Accessed June 17, 2019. http://hdl.handle.net/10027/22043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hill, Brittaney. “Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.” 2017. Web. 17 Jun 2019.

Vancouver:

Hill B. Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/10027/22043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hill B. Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/22043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

2. Ikeda, Yuko. CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES.

Degree: PhD, Department of Agricultural, Food, and Nutritional Science, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/2r36tz97r

► Immobilization has the potential to make enzyme utilization more cost efficient, but reduced enzymatic activity makes it unfeasible. The purpose of this study was to… (more)

Subjects/Keywords: Biorefinery; Cellulases; Immobilization

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APA (6^th Edition):

Ikeda, Y. (2013). CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2r36tz97r

Chicago Manual of Style (16^th Edition):

Ikeda, Yuko. “CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES.” 2013. Doctoral Dissertation, University of Alberta. Accessed June 17, 2019. https://era.library.ualberta.ca/files/2r36tz97r.

MLA Handbook (7^th Edition):

Ikeda, Yuko. “CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES.” 2013. Web. 17 Jun 2019.

Vancouver:

Ikeda Y. CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2019 Jun 17]. Available from: https://era.library.ualberta.ca/files/2r36tz97r.

Council of Science Editors:

Ikeda Y. CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/2r36tz97r

3. Mathew,P T. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.

Degree: Marine Science;, 1989, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/1224

Subjects/Keywords: Immobilization; Purification

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APA (6^th Edition):

T, M. (1989). Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/1224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

T, Mathew,P. “Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.” 1989. Thesis, Cochin University of Science and Technology. Accessed June 17, 2019. http://dyuthi.cusat.ac.in/purl/1224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

T, Mathew,P. “Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.” 1989. Web. 17 Jun 2019.

Vancouver:

T M. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. [Internet] [Thesis]. Cochin University of Science and Technology; 1989. [cited 2019 Jun 17]. Available from: http://dyuthi.cusat.ac.in/purl/1224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

T M. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. [Thesis]. Cochin University of Science and Technology; 1989. Available from: http://dyuthi.cusat.ac.in/purl/1224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

4. Tjong, C.T.F. Immobilization strategies for peptide microarrrays.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/256506

► Peptide microarrays have been developed over the last decade into a technology that can profile biomolecules. It has proven to be a versatile tool for… (more)

Subjects/Keywords: Immobilization strategies; Peptide Microarrays; Peptides

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APA (6^th Edition):

Tjong, C. T. F. (2012). Immobilization strategies for peptide microarrrays. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/256506

Chicago Manual of Style (16^th Edition):

Tjong, C T F. “Immobilization strategies for peptide microarrrays.” 2012. Masters Thesis, Universiteit Utrecht. Accessed June 17, 2019. http://dspace.library.uu.nl:8080/handle/1874/256506.

MLA Handbook (7^th Edition):

Tjong, C T F. “Immobilization strategies for peptide microarrrays.” 2012. Web. 17 Jun 2019.

Vancouver:

Tjong CTF. Immobilization strategies for peptide microarrrays. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2019 Jun 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/256506.

Council of Science Editors:

Tjong CTF. Immobilization strategies for peptide microarrrays. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/256506

Penn State University

5. Gaddes, David Edwin. Remote Calorimetric Biosensing For Clinical Diagnostic Applications.

Degree: PhD, Bioengineering, 2016, Penn State University

URL: https://etda.libraries.psu.edu/catalog/12544deg5098

► The medical community increasingly relies on quantification of biomarkers in blood, urine, and saliva to monitor treatments and screen for diseases. While glucose meters have… (more)

Subjects/Keywords: Biosensor; calorimetry; microfabrication; enzyme immobilization

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APA (6^th Edition):

Gaddes, D. E. (2016). Remote Calorimetric Biosensing For Clinical Diagnostic Applications. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/12544deg5098

Chicago Manual of Style (16^th Edition):

Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Doctoral Dissertation, Penn State University. Accessed June 17, 2019. https://etda.libraries.psu.edu/catalog/12544deg5098.

MLA Handbook (7^th Edition):

Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Web. 17 Jun 2019.

Vancouver:

Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Internet] [Doctoral dissertation]. Penn State University; 2016. [cited 2019 Jun 17]. Available from: https://etda.libraries.psu.edu/catalog/12544deg5098.

Council of Science Editors:

Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Doctoral Dissertation]. Penn State University; 2016. Available from: https://etda.libraries.psu.edu/catalog/12544deg5098

Louisiana State University

6. Calixte, Nyote J. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.

Degree: PhD, Chemistry, 2014, Louisiana State University

URL: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

► The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of… (more)

Subjects/Keywords: kinetics; microscale; nanoscale; immobilization; enzyme

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APA (6^th Edition):

Calixte, N. J. (2014). From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

Chicago Manual of Style (16^th Edition):

Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Doctoral Dissertation, Louisiana State University. Accessed June 17, 2019. etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.

MLA Handbook (7^th Edition):

Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Web. 17 Jun 2019.

Vancouver:

Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Internet] [Doctoral dissertation]. Louisiana State University; 2014. [cited 2019 Jun 17]. Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.

Council of Science Editors:

Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Doctoral Dissertation]. Louisiana State University; 2014. Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

Universidade Nova

7. Couto, Cláudia Alexandra Maia do. Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.

Degree: 2012, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145

►

Thesis for the master degree in Structural and Functional Biochemistry

The work presented here had the objective of using gold nanoparticles (AuNPs) functionalized with mercaptoundecanoic… (more)

▼

Thesis for the master degree in Structural and Functional Biochemistry

The work presented here had the objective of using gold nanoparticles (AuNPs) functionalized with mercaptoundecanoic acid (MUA), or the peptide CALNN, to develop bionanoconjugates (BNCs) with the enzyme Rhus vernicifera (Rv) laccase. Laccases are multi copper oxidases able to catalyze the oxidation of a variety of phenolic compounds with the reduction of molecular oxygen to water, and conjugating this enzyme with AuNPs could potentially contribute to enhance its catalytic activity. Spectroscopic, electrophoretic, kinetic, and computer modelling studies were carried out in order to characterize the enzyme structurally and investigate its stability and activity when conjugated with AuNPs. The studies performed revealed that Rv laccase is a monomer with 5.65 ± 0.83 nm in diameter and presents an optimal activity at pH 7.5, when syringaldazine was used as substrate. Laccase was successfully adsorbed to AuNP-CALNN but not to AuNP-MUA. BNCs with laccase and AuNP-CALNN remained active in the experimental conditions tested at pH from 6 – 8.5. Laccase followed a Michaelis-Menten kinetic model using syringaldazine as a substrate, in the pH range 6 – 8.5. Parallel studies with free laccase were conducted to evaluate the influence of AuNP-CALNN on laccase activity. These kinetic studies were performed by following substrate consumption and product formation. The kinetic results with less error were obtained when the reaction of product formation was followed. Therefore, the results for product formation were considered to be more reliable than the results obtained for substrate consumption. The results for product formation revealed that the catalytic efficiency of BNCs was more than 50% higher at pH 7, almost 100% higher at pH 7.5 and 30% higher at pH 8 in comparison with free laccase. At pH 6 there was a decrease of approximately 38% in the catalytic efficiency. The catalytic efficiency was similar in BNCs and free laccase at pH 6.5 and 8.5. In the pH range 6.5 to 8.5, AuNP-CALNN proved beneficial by either maintaining or increasing the catalytic activity of laccase. Using AuNP-CALNN to immobilize laccase might allow decreasing industrial costs, recycling the enzyme and improving the outcome of processes usually achieved with free laccase, such as delignification, wine clarification, textile dye bleaching or waste water detoxification.

Advisors/Committee Members: Franco, Ricardo.

Subjects/Keywords: Gold nanoparticles; Laccase; Bionanoconjugates; Immobilization

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APA (6^th Edition):

Couto, C. A. M. d. (2012). Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Couto, Cláudia Alexandra Maia do. “Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.” 2012. Thesis, Universidade Nova. Accessed June 17, 2019. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Couto, Cláudia Alexandra Maia do. “Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.” 2012. Web. 17 Jun 2019.

Vancouver:

Couto CAMd. Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. [Internet] [Thesis]. Universidade Nova; 2012. [cited 2019 Jun 17]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Couto CAMd. Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. [Thesis]. Universidade Nova; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

8. Fuchs, Brian K. Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.

Degree: MS, Chemical Engineering, 2015, Oregon State University

URL: http://hdl.handle.net/1957/56327

► Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20%… (more)

▼ Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20% of greenhouse gas emissions. Methane is routinely flared in many industrial processes without harnessing any of its energy. The environmental impact and wasted energy potential make it highly desirable to find an economically feasible process to use this methane. One possibility is to convert methane into liquid fuels for transportation and energy generation. Current technologies to convert methane gas to liquid fuels (GTL) are complex, and the facilities are only economical at huge scales. Methane gas is very difficult to transport and store, so GTL plants must be located at the source of the methane, typically at large petroleum fields or refineries. Biological conversion of methane to liquid fuels is an attractive alternative to traditional GTL processes, as microbial oxidation of methane can produce liquid fuels (e.g. methanol) at ambient temperatures and pressures. When biological organisms are combined with microfluidic technologies, which provide enhanced mass and heat transfer along with a high degree of process control, a very efficient conversion process can be attained at much smaller scales. A further advantage of microfluidics is that the reactors are inherently modular, allowing them to be adapted to practically any required size. This enables the economical conversion of small or remote methane streams to liquid fuels. In this thesis, techniques are described for the immobilization of Methylosinus trichosporium OB3b in a biolamina-plate microreactor (BLP) for conversion of methane to methanol. Effective immobilization requires that the cells remain viable and immobile in the reactor, and that the encapsulation medium is stable and does not degrade during reactor operation. Calcium alginate gels were identified as an ideal immobilization medium, as they are inexpensive, non-toxic, and widely used for the immobilization of cells. Three main requirements must be met in immobilization of cells in the alginate: gel cohesion, gel adhesion, and cell viability. The alginate gel must remain cohesive throughout the entire reactor process, without substantial swelling, disintegration or degradation. The alginate must also adhere stably to the reactor surface, to prevent sloughing which may cause clogging and loss of biological activity. The immobilized cells also must remain metabolically active over the duration of the reactor run. Stable, thin (300-μm) calcium alginate films were achieved by combining an “internal gelation” process to uniformly cross-link the hydrogel, electrostatic adhesion of alginate gel on stainless steel reactor plates modified with aminopropyl-trimethoxysilane (APTMS), and buffering using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) to minimize chelation of stabilizing calcium ions from the gel. The alginate-encapsulated OB3b cells retain viability and metabolic activity in these films, although their metabolism of methane to methanol… Advisors/Committee Members: Schilke, Karl F. (advisor), Remcho, Vincent T. (committee member).

Subjects/Keywords: cell immobilization; Methanotrophs – Biotechnology

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APA (6^th Edition):

Fuchs, B. K. (2015). Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/56327

Chicago Manual of Style (16^th Edition):

Fuchs, Brian K. “Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.” 2015. Masters Thesis, Oregon State University. Accessed June 17, 2019. http://hdl.handle.net/1957/56327.

MLA Handbook (7^th Edition):

Fuchs, Brian K. “Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.” 2015. Web. 17 Jun 2019.

Vancouver:

Fuchs BK. Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. [Internet] [Masters thesis]. Oregon State University; 2015. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/1957/56327.

Council of Science Editors:

Fuchs BK. Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. [Masters Thesis]. Oregon State University; 2015. Available from: http://hdl.handle.net/1957/56327

Cornell University

9. Lata, James. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/40952

► The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has… (more)

▼ The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has inherent issues with time-sensitive pathologies because of the inherently slow speed of antibodyantigen interactions. Such an obstacle can be overcome through use of faster enzymatic reactions. In this work, we focused on creating an enzymatic based PoCT diagnostic platform suitable for timesensitive pathologies, in particular addressing stroke. We hypothesize that by immobilizing enzymes to nanoparticles (NP) the enzymes will be stabilized and confined to a smaller volume, therefore increasing the efficiency of sequential enzymatic reactions needed to detect biomarkers at physiological and pathological levels in human serum and transduce that detection into a luminescent signal. Development of this platform involved several key steps, including immobilizing functional enzymes on inorganic surfaces and developing microfluidic systems with microliter-volume reactions. Because the use of immobilized enzymes in hybrid organic-inorganic devices is in a nascent stage of development, it is also essential to work out fundamental principles of how the inorganic scaffolds might affect the activities of the tethered enzymes. Through biomimicry of the sperm flagellum, enzymes were designed to include a His-tag to bind with Ni-NTA groups on an inorganic surface in a precise orientation. This approach yielded higher specific activity than did attachment of the same enzymes through adsorption or chemically-specific binding (e.g. amine-carboxyl) techniques. While the orientation had dramatic impacts on the enzyme function, the surface size/curvature to which it is bound also influenced the enzyme function. Prior literature on this relationship was largely anecdotal. To identify generalizable truths regarding the iii effects of the surface size and curvature on enzyme function, we used three enzymes, each representing a different class of enzyme, at maximal enzyme loading and in monolayers on gold NPs ranging in size from 5 to 50 nm in diameter. We found that larger NPs had increased enzyme multilayer formations and decreased kcat and specific activity by 2.8 to 8.3 fold, depending on the enzyme. Contrary to other reports and suggesting an advantage for oriented immobilization, we did not see effects of size on the same enzymes when tethered in monolayers. Tethering to NPs has immediate application in medical diagnosis of time-sensitive pathologies due to enzymes' rapid catalysis versus slow antibody interactions. Neuron specific enolase (NSE) is a biomarker for stroke, which we detected through the sequential reaction of tethered luciferase (Luc) and pyruvate kinase (PK), yielding a luminescent signal proportional to the concentration of enolase in our samples. In both rat stroke models and human patient samples, we found this diagnostic platform gave accurate results in 10 minutes compared to typical 4 hour ELISA tests, yet maintained a high… Advisors/Committee Members: Erickson,David (committeeMember), Jin,Moonsoo (committeeMember).

Subjects/Keywords: Protein Engineering; Diagnostic; Enzyme Immobilization

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APA (6^th Edition):

Lata, J. (2015). Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies .” 2015. Thesis, Cornell University. Accessed June 17, 2019. http://hdl.handle.net/1813/40952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies .” 2015. Web. 17 Jun 2019.

Vancouver:

Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/1813/40952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Akron

10. Chen, Xi. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.

Degree: MS, Polymer Science, 2015, University of Akron

URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

► Biomimetic catalysis has great impact on the development of the research of organic catalysis1. Immobilized enzyme used as catalysts has attracted a lot of attention… (more)

Subjects/Keywords: Polymers; Enzyme, immobilization, UV-vis, infrared

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APA (6^th Edition):

Chen, X. (2015). Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

Chicago Manual of Style (16^th Edition):

Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.” 2015. Masters Thesis, University of Akron. Accessed June 17, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.

MLA Handbook (7^th Edition):

Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers.” 2015. Web. 17 Jun 2019.

Vancouver:

Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. [Internet] [Masters thesis]. University of Akron; 2015. [cited 2019 Jun 17]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.

Council of Science Editors:

Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic Reaction of Enzymes and Immobilization Enzyme on Porous Polymers. [Masters Thesis]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

University of Alberta

11. Hass, Michael J. Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations.

Degree: MS, Department of Chemistry, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/n296x015t

► A method was developed to immobilize and reuse a catalyst for the asymmetric cycloisomerization of enynes. Immobilization is achieved through use of metal containing monomers,… (more)

Subjects/Keywords: cycloisomerization; rhodium; catalyst immobilization; catalysis; enantioselective

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APA (6^th Edition):

Hass, M. J. (2012). Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/n296x015t

Chicago Manual of Style (16^th Edition):

Hass, Michael J. “Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations.” 2012. Masters Thesis, University of Alberta. Accessed June 17, 2019. https://era.library.ualberta.ca/files/n296x015t.

MLA Handbook (7^th Edition):

Hass, Michael J. “Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations.” 2012. Web. 17 Jun 2019.

Vancouver:

Hass MJ. Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2019 Jun 17]. Available from: https://era.library.ualberta.ca/files/n296x015t.

Council of Science Editors:

Hass MJ. Reusable Ru and Rh catalysts for ester hydrogenations and enyne cycloisomerizations. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/n296x015t

University of Toronto

12. Odedra, Devangbhai. Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29595

►

A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We… (more)

Subjects/Keywords: gradient; VEGF; Tissue Engineering; collagen; immobilization; 0541

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Odedra, D. (2011). Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/29595

Chicago Manual of Style (16^th Edition):

Odedra, Devangbhai. “Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.” 2011. Masters Thesis, University of Toronto. Accessed June 17, 2019. http://hdl.handle.net/1807/29595.

MLA Handbook (7^th Edition):

Odedra, Devangbhai. “Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.” 2011. Web. 17 Jun 2019.

Vancouver:

Odedra D. Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/1807/29595.

Council of Science Editors:

Odedra D. Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29595

University of Toronto

13. Petryayeva, Eleonora. Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/32487

►

The unique optical properties of quantum dots (QDs) have been widely used to develop bioassays based on Fluorescence Resonance Energy Transfer (FRET). The solid-phase assays… (more)

Subjects/Keywords: quantum dots; fluorescence; hybridization; immobilization; 0486

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APA (6^th Edition):

Petryayeva, E. (2012). Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/32487

Chicago Manual of Style (16^th Edition):

Petryayeva, Eleonora. “Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.” 2012. Masters Thesis, University of Toronto. Accessed June 17, 2019. http://hdl.handle.net/1807/32487.

MLA Handbook (7^th Edition):

Petryayeva, Eleonora. “Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.” 2012. Web. 17 Jun 2019.

Vancouver:

Petryayeva E. Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/1807/32487.

Council of Science Editors:

Petryayeva E. Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/32487

Arizona State University

14. Bone, Stephanie Rosalia. Immobilization of T4 on Modified Silica Particles.

Degree: Civil and Environmental Engineering, 2017, Arizona State University

URL: http://repository.asu.edu/items/44290

► Bacteriophage provide high specificity to bacteria; receiving interest in various applications and have been used as target recognition tools in designing bioactive surfaces. Several current… (more)

Subjects/Keywords: Environmental engineering; bacteriophage; bacteriophage immobilization; silanization

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APA (6^th Edition):

Bone, S. R. (2017). Immobilization of T4 on Modified Silica Particles. (Masters Thesis). Arizona State University. Retrieved from http://repository.asu.edu/items/44290

Chicago Manual of Style (16^th Edition):

Bone, Stephanie Rosalia. “Immobilization of T4 on Modified Silica Particles.” 2017. Masters Thesis, Arizona State University. Accessed June 17, 2019. http://repository.asu.edu/items/44290.

MLA Handbook (7^th Edition):

Bone, Stephanie Rosalia. “Immobilization of T4 on Modified Silica Particles.” 2017. Web. 17 Jun 2019.

Vancouver:

Bone SR. Immobilization of T4 on Modified Silica Particles. [Internet] [Masters thesis]. Arizona State University; 2017. [cited 2019 Jun 17]. Available from: http://repository.asu.edu/items/44290.

Council of Science Editors:

Bone SR. Immobilization of T4 on Modified Silica Particles. [Masters Thesis]. Arizona State University; 2017. Available from: http://repository.asu.edu/items/44290

King Abdullah University of Science and Technology

15. Lloyd-Randol, Jennifer D. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.

Degree: 2012, King Abdullah University of Science and Technology

URL: http://hdl.handle.net/10754/224713

► The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1].… (more)

▼ The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1]. Biocatalytic energy conversion is a promising solution to meet the increased energy demand of industrialized societies. Applications of biocatalysis in the gas-phase are so far limited to production of fine chemicals and pharmaceuticals. However, this technology has the potential for large scale biocatalytic applications [2], e.g. for the formation of novel energy carriers. The so-called solid/gas biocatalysis is defined as the application of a biocatalyst immobilized on solid-phase support acting on gaseous substrates [3]. This process combines the advantages of bio-catalysis (green chemistry, mild reaction conditions, high specicity & selectivity) and heterogeneous dynamic gas-phase processes (low diffusion limitation, high conversion, simple scale-up). This work presents the modifications of a PID Microactivity Reference reactor in order to make it suitable for solid/gas biocatalysis. The reactor design requirements are based on previously published laboratory scale solid/gas systems with a feed of saturated vapors [4]. These vapors are produced in saturation flasks, which were designed and optimized during this project. Other modifications included relocation of the gas mixing chamber, redesigning the location and heating mechanism for the reactor tube, and heating of the outlet gas line. The modified reactor system was verified based on the Candida antarctica lipase B catalyzed transesterication of ethyl acetate with 1-hexanol to hexyl acetate and ethanol and results were compared to liquid-phase model reactions. Products were analyzed on line by a gas chromatograph with a flame ionization detector. C. antarc- tica physisorbed on silica particles produced a 50% conversion of hexanol at 40 C in the gas-phase. A commercial immobilized lipase from Iris Biotech produced 99% and 97% conversions of hexanol in similar experiments. This project achieved its goal to design, establish and successfully verify a solid/- gas biocatalysis reactor. Future work will target optimization of the reactor's operating conditions and the development of whole cell catalysts for energy production reactions. Potential experiments include the study of hydrogenolytic carbon dioxide reduction to methanol by free enzymes or methanogenic organisms [5], and the investigation of hydrogen production by water splitting of algae or cyanobacteria.

Subjects/Keywords: Biocatalytic; Validation; Solid gas; Bioreactor; Immobilization

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APA (6^th Edition):

Lloyd-Randol, J. D. (2012). Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/224713

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lloyd-Randol, Jennifer D. “Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.” 2012. Thesis, King Abdullah University of Science and Technology. Accessed June 17, 2019. http://hdl.handle.net/10754/224713.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lloyd-Randol, Jennifer D. “Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.” 2012. Web. 17 Jun 2019.

Vancouver:

Lloyd-Randol JD. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2012. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/10754/224713.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lloyd-Randol JD. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. [Thesis]. King Abdullah University of Science and Technology; 2012. Available from: http://hdl.handle.net/10754/224713

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

16. Misson, Mailin. Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/103557

► Enzyme immobilization has been recognized as a promising technique to enhance enzyme stability, activity and reusability for the development of cost-effective, green and sustainable biotechnological… (more)

▼ Enzyme immobilization has been recognized as a promising technique to enhance enzyme stability, activity and reusability for the development of cost-effective, green and sustainable biotechnological processes. Recent development in nanotechnology has opened a new frontier for diverse nano-scale enzyme carriers. The immobilization of enzyme onto nanomaterials produces a nanobiocatalyst assembly, which maximizes reaction efficiencies by favoring desirable chemical reaction kinetics and selectivity for substrates, while the unique properties of nanocarriers offer a revolution of biocatalyst applications in the bioprocessing field. Nevertheless, the issues of enzyme leakage and conformational changes make the translation of the biocatalyst technology into commercial practices technically challenging and economically infeasible. Hence, investigating new technologies for fabricating the nanobiocatalyst with promising biocatalytic activities and functionalities is of great importance. In this PhD research, nanoparticle- and nanofiber-based enzyme carriers were developed and explored to immobilize β-galactosidase for conversion of lactose from dairy industry wastes into galacto-oligosaccharide (GOS) as a high value product. The structure-function relationship for the nanocarrier, the enzyme-nanocarrier microenvironment and the enzyme-nanocarrier nanobiocatalyst structure were extensively evaluated, aiming to enhance the bioengineering performance of the nanobiocatalysts. Dendrimer-like silica nanoparticles (HPSNs) with hierarchical pores were synthesized, characterized and functionalized with amino (NH2) and carboxyl group (COOH) to facilitate enzyme binding. Our findings revealed that surface functionalization can promote enzyme affinity towards the nanomaterial interface and selectively enhance enzyme reusability and its catalytic activity for improving the GOS production yield. A systematic synthesis of polystyrene nanofibers (PSNFs) was executed by optimizing key fabrication parameters using the electrospinning technique, including polymer concentration, electric voltage and distance between discharge needle tips and the collector. Surface modification of the PSNF was found to improve enzyme loading and activity. In addition, the local microenvironment of the nanobiocatalysts was able to optimize the enzyme selectivity and specificity, resulting in favouring transgalactosylation over hydrolysis for the lactose bioconversion. Further investigation to enhance the enzyme stability and catalytic activity at various operating conditions was conducted. PSNFs were chosen as the enzyme carrier owing to their scaling up potential in a manufacturing reactor system with their excellent mechanical and structural properties. Immobilizing β-galactosidase on the modified PSNF surface facilitated formation of stable enzyme binding and exhibited distinguished catalytic performance. Thermal and pH stability were improved significantly while the recyclability was enhanced from four to nine cycles. The evaluation of lactose conversion… Advisors/Committee Members: Zhang, Hu (advisor), Jin, Bo (advisor), School of Chemical Engineering (school).

Subjects/Keywords: nandbiocatalysts; immobilization; β-galactosidase; nanofibers; nanoparticles; bioprocess

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APA (6^th Edition):

Misson, M. (2016). Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Misson, Mailin. “Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.” 2016. Thesis, University of Adelaide. Accessed June 17, 2019. http://hdl.handle.net/2440/103557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Misson, Mailin. “Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.” 2016. Web. 17 Jun 2019.

Vancouver:

Misson M. Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/2440/103557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Misson M. Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/103557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Uppsala University

17. Schönn, Mikael Andersson. Promoter regulation : designing cells for biotechnological applications.

Degree: Molecular Biomimetics, 2016, Uppsala University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502

► The filamentous cyanobacteria Nostoc punctiforme ATCC 29133 is a model species fordevelopment of sustainable production methods of numerous compounds. One of its uniquefeatures is… (more)

Subjects/Keywords: Cyanobacteria; heterocyst; promoter; specificity; confocal microscopy; immobilization

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APA (6^th Edition):

Schönn, M. A. (2016). Promoter regulation : designing cells for biotechnological applications. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Schönn, Mikael Andersson. “Promoter regulation : designing cells for biotechnological applications.” 2016. Thesis, Uppsala University. Accessed June 17, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Schönn, Mikael Andersson. “Promoter regulation : designing cells for biotechnological applications.” 2016. Web. 17 Jun 2019.

Vancouver:

Schönn MA. Promoter regulation : designing cells for biotechnological applications. [Internet] [Thesis]. Uppsala University; 2016. [cited 2019 Jun 17]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Schönn MA. Promoter regulation : designing cells for biotechnological applications. [Thesis]. Uppsala University; 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

18. Radovanović, Mirjana N., 1984-. Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином.

Degree: Tehnološko-metalurški fakultet, 2018, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get

►

Технолошко инжењерство - Биохемијско инжењерство и биотехнологија / Engineering technology - Biochemical Engineering and Biotechnology

Циљ ове дисертације био је имобилизација α-амилазе на полианилину и… (more)

▼

Технолошко инжењерство - Биохемијско инжењерство и биотехнологија / Engineering technology - Biochemical Engineering and Biotechnology

Циљ ове дисертације био је имобилизација α-амилазе на полианилину и магнетним честицама модификованим полианилином методом адсорпције ради добијања вишефункционалног биокатализаторског система погодног за вишекратну хидролизу скроба. Истраживања у оквиру ове тезе могу се поделити у три дела. У првом делу рада извршена је синтеза и карактеризација честица носача и карактеризација α-амилазe из Bacillus licheniformis ензимског препарата Termamyl. Магнетне честице (MAG) синтетисане су копреципитацијом FeCl3 и FeSO4 у базној средини, а затим обложене полианилинском превлаком (MAG-PANI). Честице полианилина (PANI) синтетисане су хемијски на граници фаза двофазног система вода/хлороформ. Испитане су морфолошке особине, магнетна својства, IR спектри, расподела величине честица и зета потенцијала MAG и MAG-PANI. Показано је да су синтетисани агломерати наночестица MAG и MAG-PANI. Површина магнетних честица униформно је пресвучена полианилином влакнасте структуре. Честице MAG карактерише бимодална, а MAG-PANI тримодална расподела величина честица. У другом делу рада извршена је имобилизација ензима α-амилазе на честицaма MAG, MAG-PANI и PANI. Одређени су оптимални услови имобилизације на MAG-PANI: време, почетна концентрација ензима, рН и температура. Испитивањем кинетичких параметара Km и Vm утврђено је да имобилисана α-амилаза на МАG-PANI показује већи афинитет према супстрату него имобилисана α-амилаза на MAG, а нижи афинитет од слободне α-амилазе. Утврђен је Ленгмиров (Langmuir) модел адсорпције α-амилазе на честицама MAG-PANI. У трећем делу рада испитана је стабилност имобилисане α-амилазе, могућност употребе кроз више циклуса као и оперативна стабилност у проточном биореактору са континуалним и повратним током. Утврђена је рН стабилизација α-амилазе имобилизацијом на MAG честицама и померање температурног оптимума имобилизацијом на MAG-PANI. Имобилисана α-амилаза на MAG-PANI задржала је око 55,5% почетне активности након 9 циклуса коришћења и око 80,3% почетне активности након 50 дана чувања. Време полу-живота биокатализатора при континуалним условима рада било је 6,2 h.

Advisors/Committee Members: Knežević-Jugović, Zorica, 1969-.

Subjects/Keywords: α-amylase; immobilization; magnetic particles; polyaniline

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APA (6^th Edition):

Radovanović, Mirjana N., 1. (2018). Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Radovanović, Mirjana N., 1984-. “Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином.” 2018. Thesis, Univerzitet u Beogradu. Accessed June 17, 2019. https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Radovanović, Mirjana N., 1984-. “Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином.” 2018. Web. 17 Jun 2019.

Vancouver:

Radovanović, Mirjana N. 1. Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2019 Jun 17]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Radovanović, Mirjana N. 1. Имобилизација алфа-амилазе на полианилину и магнетним честицама модификованим полианилином. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

19. Andler, Stephanie. Immobilized Enzymes for Waste Stream Valorization .

Degree: 2018, Cornell University

URL: http://hdl.handle.net/1813/59598

► Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes… (more)

▼ Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes provide a selective and greener route of synthesis, but lack stability in extreme environments, and the ability to be reused. Therefore, the present work studied immobilized enzymes for practical application in two major waste streams: oil and dairy. First, lipase B from Candida antarctica (E.C. 3.1.1.3) was immobilized within cross-linked microparticles (CLMP) displaying varying scales of support, and the influence of these nano- and macroscale support materials on lipase activity and stability was studied. Kinetic analysis and extreme environment testing revealed that that polydicyclopentadiene macrostructure had the greatest influence on stability. These CLMPs successfully produced surfactants from lauric acid and D-glucose. However, the ~10 µm particles were unrecoverable from viscous solvents. Next, lipase was immobilized into macroscale, polymerized high internal phase emulsions (HIPE) to facilitate ease of handling. After fabrication, HIPEs displayed an increase in activity (139±9.7%) over native lipase. However, lipase was weakly immobilized, and retained less than 10% relative activity after 5 washes. HIPEs retained above 40% relative activity after exposure to pH 3, 7, and 10 for 24 hours, effectively expanding the working range of lipase. HIPEs also displayed an increase in relative activity after storage at 20 ˚C and 40 ˚C for 24 hours and retained greater than 40% activity after storage in 50 ˚C and 60 ˚C for 24 hours. These macroscale HIPEs were recoverable from reaction media but require further optimization to increase protein retention. Finally, β-galactosidase from Aspergillus oryzae (E.C. 3.2.1.23) and glucose isomerase (E.C. 5.3.1.5) were co-immobilized within a cross-linked enzyme aggregate (combi-CLEA) for dairy waste upcycling. The β-galactosidase within the combi-CLEA retained approximately 10% activity after immobilization, but activity was unable to be detected from glucose isomerase. Additional optimization of the combi-CLEA is necessary before application testing in whey permeate. Applications-based approaches to enzyme immobilization are necessary to create low-cost, industrially relevant catalysts. Through the understanding of support material influences, and extreme environment testing, enzymes have the potential to increase the overall sustainability of food processing. Advisors/Committee Members: Sacks, Gavin Lavi (committeeMember), Nugen, Sam Rasmussen (committeeMember).

Subjects/Keywords: bioprocessing; enzyme; immobilization; waste stream; Food science

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APA (6^th Edition):

Andler, S. (2018). Immobilized Enzymes for Waste Stream Valorization . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/59598

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization .” 2018. Thesis, Cornell University. Accessed June 17, 2019. http://hdl.handle.net/1813/59598.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization .” 2018. Web. 17 Jun 2019.

Vancouver:

Andler S. Immobilized Enzymes for Waste Stream Valorization . [Internet] [Thesis]. Cornell University; 2018. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/1813/59598.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Andler S. Immobilized Enzymes for Waste Stream Valorization . [Thesis]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59598

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Politècnica de València

20. Tortajada Serra, Marta. Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation .

Degree: 2012, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/16800

► El objetivo general de la presente tesis doctoral es el desarrollo de herramientas para la obtencion, produccion y aplicacion de dos enzimas glicosidicas: �¿-L-arabinofuranosidasa proveniente… (more)

Subjects/Keywords: Glycosidases; Pichia pastoris; Constraint-based model; Immobilization

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APA (6^th Edition):

Tortajada Serra, M. (2012). Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/16800

Chicago Manual of Style (16^th Edition):

Tortajada Serra, Marta. “Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation .” 2012. Doctoral Dissertation, Universitat Politècnica de València. Accessed June 17, 2019. http://hdl.handle.net/10251/16800.

MLA Handbook (7^th Edition):

Tortajada Serra, Marta. “Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation .” 2012. Web. 17 Jun 2019.

Vancouver:

Tortajada Serra M. Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2012. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/10251/16800.

Council of Science Editors:

Tortajada Serra M. Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation . [Doctoral Dissertation]. Universitat Politècnica de València; 2012. Available from: http://hdl.handle.net/10251/16800

University of New South Wales

21. Hou, Jingwei. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.

Degree: Chemical Sciences & Engineering, 2014, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

► Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant… (more)

▼ Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant and greenhouse gas, which require novel mitigation approaches. Enzyme catalyzed reactions have been recognized as promising approaches for many environmental applications. Among different enzymes, laccase and carbonic anhydrase have attracted wide spread interest in wastewater treatment and CO2 capture respectively. However, the application of free enzyme is restricted due to the difficulty in recycle and reuse. Therefore, efficient enzyme immobilization and bio-reactor design are required.Based on a series of comprehensive experimental researches, the performance of TiO2 nanoparticle and TiO2 nanoparticle functionalized membrane as laccase immobilization support has been evaluated. Different immobilization techniques were compared and the sequential process was regarded as the optimal option due to its relatively high activity and stability. It was also found that the nanoparticle size, surface charge, and laccase accessibility also had significant effect on the immobilized laccase performance.TiO2 functionalized membranes were prepared with both nanoparticle blending and sol-gel coating processes, then laccase was immobilized on the resultant membranes. Compared with the blended membrane, the coated membrane provided more accessible TiO2 for laccase immobilization thus resulted in better performances. The effect of coating layer surface architectural properties and the membrane pore size on the laccase immobilization performance was also discussed.In this study, two laccase based membrane bioreactors were proposed for bisphenol-A (BPA) degradation: the bio-catalytic membrane reactor and the bio-catalytic nanoparticle suspension membrane hybrid reactor. For both reactors, satisfactory BPA removal was achieved through enzymatically degradation and membrane filtration process. In addition, the performance of the hybrid reactor under simulated wastewater harsh conditions was assessed to evaluate the feasibility of using such reactors for municipal water treatment.Finally, the feasibility of applying the TiO2 nanoparticle and TiO2 coated polymer membrane as carbonic anhydrase immobilization support was also explored. This study also prepared a novel TiO2 functionalized hydrophilic-hydrophobic membrane for carbonic anhydrase immobilization. Such a bio-catalytic membrane was further used for CO2 hydration with a gas-liquid contactor, and nearly 100 % improvement in CO2 hydration rate was obtained Advisors/Committee Members: CHEN, VICKI, Chemical Sciences & Engineering, Faculty of Engineering, UNSW.

Subjects/Keywords: CO2 hydration; Enzyme immobilization; Micropollutant removal

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APA (6^th Edition):

Hou, J. (2014). Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Doctoral Dissertation, University of New South Wales. Accessed June 17, 2019. http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Web. 17 Jun 2019.

Vancouver:

Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Internet] [Doctoral dissertation]. University of New South Wales; 2014. [cited 2019 Jun 17]. Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.

Council of Science Editors:

Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Doctoral Dissertation]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

22. Ogorzalek, Tadeusz L. Examining the Behavior of Surface Tethered Enzymes.

Degree: PhD, Chemical Biology, 2015, University of Michigan

URL: http://hdl.handle.net/2027.42/111541

► Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how… (more)

▼ Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how enzymes and proteins interact with abiological surfaces on the molecular level remains extremely limited. We have compared the structure, activity and thermal stability of beta-galactosidase variants attached to a chemically well-defined self assembled monolayer (SAM) surface. Maleimide-terminated ethylene glycol linkers were used to attach beta-galactosidase through a unique cysteinyl residue. These maleimide-terminated linkers were mixed with ethylene glycol linkers terminated with different chemical moieties to engineer surfaces with varying hydrophobicity and electrostatic charge. In collaboration with the Chen Lab, we used SFG and ATR-FTIR to experimentally measure the orientation of the surface tethered enzyme. In collaboration with the Brooks Lab, we conducted coarse grain model simulations to examine the atomic level interactions between the protein and the surface. Through coarse grain modelling, it was shown that the increased range of motion allowed to an enzyme tethered to a flexible loop region increased the number of protein surface interactions relative to the interactions experienced by an enzyme attached to the helix. For mildly hydrophibic surfaces, such as a full maleimide-terminated SAM, these increased interactions are destabilizing. This was confirmed experimentally by the reduction in thermal stability for beta-galactosidase attached by the loop region. Using SFG, it was shown that the distribution of orientations of an enzyme attached to a loop is greater than to an enzyme tethered to a helix. By varying the electrostatic properties of the terminal groups used in the SAMs, it was shown that beta-galactosidase immobilized onto SAMs containing a mixture of positively and negatively terminated residues retained a higher level of specific activity than surfaces terminated with either uncharged hydrophilic or hydrophobic surfaces. Thermal stability was highest on uncharged hydrophilic surfaces. Overall, we were able to develop a molecular level model for the behavior of surface attached enzymes, and a potential approach for predicting approaches for engineering tethered enzyme systems. Advisors/Committee Members: Marsh, E Neil G. (committee member), Chen, Zhan (committee member), Walter, Nils G. (committee member), O'Brien, Patrick (committee member).

Subjects/Keywords: Enzyme Immobilization; Surface Attachment; Biological Chemistry; Science

10 more images…

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APA (6^th Edition):

Ogorzalek, T. L. (2015). Examining the Behavior of Surface Tethered Enzymes. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/111541

Chicago Manual of Style (16^th Edition):

Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Doctoral Dissertation, University of Michigan. Accessed June 17, 2019. http://hdl.handle.net/2027.42/111541.

MLA Handbook (7^th Edition):

Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Web. 17 Jun 2019.

Vancouver:

Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/2027.42/111541.

Council of Science Editors:

Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/111541

University of New South Wales

23. Tregubov, Andrey Aleksandrovich. Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.

Degree: Chemistry, 2013, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true

► This thesis describes the study of the immobilization of Rh(I) complexes with N,N (bis(pyrazol-1-yl)methane (bpm), bis(N-methylimidazol-2-yl)methane (bim), and 4-(pyrazol-1-yl)methyl)-1-H-1,2,3-triazole (PyT)) and N,P (1-[2-(diphenylphosphino)ethyl]pyrazole (dmPyP) ligands… (more)

▼ This thesis describes the study of the immobilization of Rh(I) complexes with N,N (bis(pyrazol-1-yl)methane (bpm), bis(N-methylimidazol-2-yl)methane (bim), and 4-(pyrazol-1-yl)methyl)-1-H-1,2,3-triazole (PyT)) and N,P (1-[2-(diphenylphosphino)ethyl]pyrazole (dmPyP) ligands onto carbon surfaces (glassy carbon electrode (GC), glassy carbon beads (GCB), carbon black (XC)) via robust carbon-carbon bonds. The immobilized Rh(I) complexes were employed as recoverable catalysts for hydromamination and hydroalkoxylation reactions.A series of aforementioned N,N ligands and one N,P ligand precursor were functionalized with aniline and aniline hydrochloride respectively. The treatment of the aniline-functionalized ligands and aniline hydrochloride-functionalized ligand precursor with HNO2 resulted in the transformation of the NH2 functionality into diazonium one. The electrochemical reduction of the diazonium functionality of the N,N ligands and N,P ligand precursor resulted in the surface attachment of these compounds onto glassy carbon electrode surfaces. The treatment of the surface-bound N,P ligand precursor with LiPPh2 resulted in the formation of the surface-bound N,P ligand (dmPyP). The complexation of [Rh(CO)2(μ-Cl)]2 with the immobilized N,N and N,P ligands led to the formation of immobilized Rh(I) complexes of these ligands. XPS confirmed the surface attachment of the N,N ligands and N,P ligand precursor as well as the success of the synthesis of the N,P ligand on the surface and complexation between immobilized ligands and the Rh precursor. The surface coverage of immobilized Rh(I) complexes was evaluated using CV and was found to be close to being a monolayer. The immobilized Rh(I) complexes were active as catalysts for the intramolecular hydroamination and dihydroalkoxylation reactions with extremely high TONs in comparison with their homogeneous counterparts. Studies of the recovered catalyst samples showed that Rh leached from the surface while the ligand remained intact. The aniline-functionalized N,N ligands were also attached onto glassy carbon electrodes without electrochemical assistance by dipping the electrode into a solution of aryl diazonium salt. Complexation of the ligands immobilized without electrochemical assistance and [Rh(CO)2(μ-Cl)]2 resulted in immobilized Rh(I) complexes with these ligands. XPS confirmed that the immobilized species had the same nature as those immobilized using electrochemical reduction and they were effective catalysts for dihydroalkoxylation. The surface coverage of these complexes was evaluated by means of CV and was found to be close to being a monolayer. The ligand bpm was also immobilized onto carbon powders (GCB and XC) without electrochemical assistance. The immobilized support-[Rh(bpm)(CO)2] complexes were obtained by the treatment of bpm-functionalized supports with [Rh(CO)2(μ-Cl)]2. XPS confirmed the ligand attachment onto the surface of carbon powders and complexation with Rh. TGA also confirmed the covalent immobilization of the ligand and was used to determine the… Advisors/Committee Members: Messerle, Barbara, Chemistry, Faculty of Science, UNSW.

Subjects/Keywords: Solid-state NMR; Catalyst immobilization; Electrochemistry; Carbon

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APA (6^th Edition):

Tregubov, A. A. (2013). Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Tregubov, Andrey Aleksandrovich. “Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.” 2013. Doctoral Dissertation, University of New South Wales. Accessed June 17, 2019. http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Tregubov, Andrey Aleksandrovich. “Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.” 2013. Web. 17 Jun 2019.

Vancouver:

Tregubov AA. Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2019 Jun 17]. Available from: http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true.

Council of Science Editors:

Tregubov AA. Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true

24. Reni, George. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.

Degree: Applied Chemistry, 2013, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/4627

►

Mesoporous silica nanoparticles provide a non-invasive and biocompatible delivery platform for a broad range of applications in therapeutics, pharmaceuticals and diagnosis. Additionally, mesoporous silica materials… (more)

Subjects/Keywords: Enzymes as biocatalyst; Immobilization; ideal enzyme supports; Enzyme immobilization; activity of enzymes; Enzyme adsorption isotherms

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APA (6^th Edition):

Reni, G. (2013). Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Thesis, Cochin University of Science and Technology. Accessed June 17, 2019. http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Web. 17 Jun 2019.

Vancouver:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Internet] [Thesis]. Cochin University of Science and Technology; 2013. [cited 2019 Jun 17]. Available from: http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Thesis]. Cochin University of Science and Technology; 2013. Available from: http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Navya, Antony. Immobilization of Diastase α-amylase on to synthetic and natural polymers.

Degree: Applied Chemistry, 2014, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/4696

►

Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization. Synthetic polymer materials are prepared by chemical polymerization using various monomers.… (more)

Subjects/Keywords: Biocatalysis; Enzymes; Nature’s catalysts; Enzyme immobilization; Methods of enzyme immobilization; Synthetic polymer-polypyrrole

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APA (6^th Edition):

Navya, A. (2014). Immobilization of Diastase α-amylase on to synthetic and natural polymers. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Navya, Antony. “Immobilization of Diastase α-amylase on to synthetic and natural polymers.” 2014. Thesis, Cochin University of Science and Technology. Accessed June 17, 2019. http://dyuthi.cusat.ac.in/purl/4696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Navya, Antony. “Immobilization of Diastase α-amylase on to synthetic and natural polymers.” 2014. Web. 17 Jun 2019.

Vancouver:

Navya A. Immobilization of Diastase α-amylase on to synthetic and natural polymers. [Internet] [Thesis]. Cochin University of Science and Technology; 2014. [cited 2019 Jun 17]. Available from: http://dyuthi.cusat.ac.in/purl/4696.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Navya A. Immobilization of Diastase α-amylase on to synthetic and natural polymers. [Thesis]. Cochin University of Science and Technology; 2014. Available from: http://dyuthi.cusat.ac.in/purl/4696

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

26. Tran, Clara. Plasma activated polymers for yeast and enzyme immobilization .

Degree: 2013, University of Sydney

URL: http://hdl.handle.net/2123/10127

► This thesis investigates plasma immersion ion implantation (PIII) for immobilizing enzymes and yeast cells with the aim of developing new processes for cellulosic ethanol production.… (more)

Subjects/Keywords: yeast immobilization; enzyme immobilization; plasma immersion ion implantation; cellulosic ethanol; surface charge

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APA (6^th Edition):

Tran, C. (2013). Plasma activated polymers for yeast and enzyme immobilization . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/10127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tran, Clara. “Plasma activated polymers for yeast and enzyme immobilization .” 2013. Thesis, University of Sydney. Accessed June 17, 2019. http://hdl.handle.net/2123/10127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tran, Clara. “Plasma activated polymers for yeast and enzyme immobilization .” 2013. Web. 17 Jun 2019.

Vancouver:

Tran C. Plasma activated polymers for yeast and enzyme immobilization . [Internet] [Thesis]. University of Sydney; 2013. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/2123/10127.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tran C. Plasma activated polymers for yeast and enzyme immobilization . [Thesis]. University of Sydney; 2013. Available from: http://hdl.handle.net/2123/10127

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Leonardo JosÃ BrandÃo Lima de Matos. Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate.

Degree: PhD, 2014, Universidade Federal do Ceará

URL: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14401 ;

►

Immobilization of enzymes provides advantages such as increased in: stability, level of process control, yield and purity of the final product, in addition to allowing… (more)

▼

Immobilization of enzymes provides advantages such as increased in: stability, level of process control, yield and purity of the final product, in addition to allowing the use of different reactor configurations. In this study, two types of support, an organic polymer (polimetilmetacrilato- PMMA) and other inorganic (silica) were used for the immobilization of lipase Candida antarctica type B (CALB). Different alcohols (ethanol, iso-propanol, n-butanol) were evaluated as agents for optimization of the immobilization process, the concentrations of 5, 10 and 15% (volume of alcohol Ã 100 / solution volume immobilization). Immobilization of PMMA was conducted using two different protocols here denominated first and second protocol generation. The best results of hydrolytic activity and esterification to the derivative of the first generation was 3,91 Â 0,09 UpNPB/g. of catalyst e 409,5 Â 19,7 Uoleic acid/g. of catalyst, respectively. When using the protocol of the second generation, the best results were obtained when aa immobilization was carried out in the presence of 5% TritonÂ X-100 2 mM, rising rate of the esterification reaction between the derivatives studied was highly significant with a higher ratio of 3,81 times to the derivative prepared with TritonÂ X-100. The CALB was also immobilized for the macroporous silica support (IB S60SÂ - Chiralvision), the activate agent used was Triethoxy(octyl)silane (C8-TEOS). The results observed for immobilization in the presence of 5% n-butanol were the best for both hydrolytic activity, being four times higher than the activity for the immobilized derivative without alcohol, as for the esterification activity was 985,1 Â 5,6 Uoleic acid/g. of catalyst . All observed derivatives supported on silica and PMMA reached a level conversion for equilibrium esterification reaction for a reaction time of 24 h the best results varying between 80 to 90%.

A imobilizaÃÃo das enzimas traz vantagens como: aumento da estabilidade, do nÃvel de controle do processo, do rendimento e da pureza do produto final, alÃm de possibilitar o uso de reatores de diferentes configuraÃÃes. Neste trabalho, dois tipos de suporte, um polÃmero orgÃnico (polimetilmetacrilato- PMMA) e outro inorgÃnico (sÃlica), foram utilizados para a imobilizaÃÃo da lipase do tipo B de Candida antarctica (CALB). Diferentes Ãlcoois (etanol, iso-propanol, n-butanol) foram avaliados como agentes de otimizaÃÃo do processo de imobilizaÃÃo, nas concentraÃÃes de 5, 10 e 15 % (volume de Ãlcool Ã100 / volume da soluÃÃo de imobilizaÃÃo). A imobilizaÃÃo em PMMA foi conduzida atravÃs de dois protocolos diferentes, aqui denominados de protocolo de primeira e segunda geraÃÃo. Os melhores resultados de atividade hidrolÃtica e de esterificaÃÃo para o derivado de primeira geraÃÃo foi de 3,91 Â 0,09 UpNPB/g. de catalisador e 409,5 Â 19,7 UÃc. olÃico/g. de catalisador, respectivamente. Quando se utilizou o protocolo de segunda geraÃÃo, melhores resultados foram obtidos quando a a imobilizaÃÃo foi conduzida na presenÃa de n-butanol 5 % e TritonÂ…

Advisors/Committee Members: Diana Cristina Silva de Azevedo, Luciana Rocha Barros GonÃalves, Hosiberto Batista de Sant'Ana, Fabiano AndrÃ Narciso Fernandes, James Almada da Silva, Nair do Amaral Sampaio Neta.

Subjects/Keywords: ENGENHARIA QUIMICA; Enzimas - ImobilizaÃÃo; EsterificaÃÃo; SÃlica; Polimetilmetacrilato; PolÃmeros; Enzymatic immobilization; Enzymatic esterification; Macroporous support; Additives for the immobilization.

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APA (6^th Edition):

Matos, L. J. B. L. d. (2014). Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate. (Doctoral Dissertation). Universidade Federal do Ceará. Retrieved from http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14401 ;

Chicago Manual of Style (16^th Edition):

Matos, Leonardo JosÃ BrandÃo Lima de. “Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate.” 2014. Doctoral Dissertation, Universidade Federal do Ceará. Accessed June 17, 2019. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14401 ;.

MLA Handbook (7^th Edition):

Matos, Leonardo JosÃ BrandÃo Lima de. “Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate.” 2014. Web. 17 Jun 2019.

Vancouver:

Matos LJBLd. Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate. [Internet] [Doctoral dissertation]. Universidade Federal do Ceará 2014. [cited 2019 Jun 17]. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14401 ;.

Council of Science Editors:

Matos LJBLd. Immobilization of lipase type B Candida antarctica in macroporous silica and polymethylmethacrylate aimed at the synthesis ethyl oleate. [Doctoral Dissertation]. Universidade Federal do Ceará 2014. Available from: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14401 ;

28. Dupin, Lucie. Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa.

Degree: Docteur es, Ingénierie pour le vivant, 2016, Lyon

URL: http://www.theses.fr/2016LYSEC018

►

Pseudomonas aeruginosa (PA) est la troisième bactérie impliquée dans les maladies nosocomiales et est la principale cause de mortalité des patients atteints de la mucoviscidose.… (more)

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Pseudomonas aeruginosa (PA) est la troisième bactérie impliquée dans les maladies nosocomiales et est la principale cause de mortalité des patients atteints de la mucoviscidose. PA est résistante à la plupart des traitements antibiotiques. Trouver de nouvelles stratégies thérapeutiques est devenu un enjeu majeur de santé publique, l’une d’entre elles est l’inhibition de facteurs de virulence. Parmi ceux-ci, les lectines sont des protéines impliquées dans l’adhésion et la formation de biofilm via des interactions avec des sucres (PA-IL, PA- IIL, FliC, FliD, PilA, PilY1 et CupB6).Le but de ce travail est donc de trouver des leurres moléculaires ayant une forte affinité pour ces lectines. Ceux-ci sont des motifs saccharidiques présentés de façon multivalente : glycoclusters. De part leur grande diversité structurale et leur faible quantité, un outil de criblage innovant a été développé qui consiste en une lame de verre microstructurée : le glycocluster-microarray. Les glycoclusters sont immobilisés de manière ordonnée par DNA Directed Immobilization (DDI). Deux méthodes de criblage ont été développées grâce à cet outils : 1) le criblage en solution et par compétition d’une bibliothèque de motifs saccharidiques et 2) le criblage d’une bibliothèque de glycoclusters immobilisés sur le microarray. Avec cet outil, des protocoles de mesures d’IC50 et de Kd ont aussi été fiabilisés pour caractériser les meilleurs candidats inhibiteurs des lectines. Le glycocluster- microarray présente l’avantage de n’utiliser qu’une très faible quantité de matériel (quelques picomoles) et permet de réaliser diverses analyses en parallèle.Afin de valider cet outil, une étude sur l’impact de la densité de surface en glycocluster a été menée. Le criblage de plus de 150 motifs saccharidiques a permis de sélectionner ceux ayant une forte affinité pour les lectines. L’analyse sur microarray complétée par de la modélisation moléculaire d’une bibliothèque de glycoclusters, possédant ces motifs et différentes topologies, valences et propriétés (aromaticité, charge,…), a permis d’identifier les paramètres clés dirigeant les relations structure-affinité. Une activité anti-biofilm chez PA a été démontrée avec les meilleurs glycoclusters ciblant PA-IL.Tester l’activité in vivo, chez l’animal, des meilleurs candidats est une voie à explorer. Cibler d’autres lectines comme celles présentes sur le flagelle et les pili de PA et notamment impliquées dans son adhésion précoce est aussi une voie à développer. Pour cela, des tests préliminaires ont été présentés et d’autres sont en cours faisant appel à l’utilisation de bactéries entières ainsi qu’à une détection sans marquage des lectines.

Summary: Pseudomonas aeruginosa (PA) is the third pathogen involved in nosocomial diseases and the major cause of mortality of cystic fibrosis patients. PA develops resistance to antibiotics treatments. And so, developing new therapeutic strategies is a public health issue. One of the promising strategies is to inhibit virulence factors involved in the adhesion and the…

Advisors/Committee Members: Chevolot, Yann (thesis director).

Subjects/Keywords: Interaction glycocluster/lectine; Microarray; DNA Directed Immobilization (DDI); Modélisation moléculaire; Glycocluster/lectin interaction; Microarray; DNA Directed Immobilization (DDI); Molecular simulations

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dupin, L. (2016). Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2016LYSEC018

Chicago Manual of Style (16^th Edition):

Dupin, Lucie. “Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa.” 2016. Doctoral Dissertation, Lyon. Accessed June 17, 2019. http://www.theses.fr/2016LYSEC018.

MLA Handbook (7^th Edition):

Dupin, Lucie. “Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa.” 2016. Web. 17 Jun 2019.

Vancouver:

Dupin L. Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa. [Internet] [Doctoral dissertation]. Lyon; 2016. [cited 2019 Jun 17]. Available from: http://www.theses.fr/2016LYSEC018.

Council of Science Editors:

Dupin L. Validation et criblage de nouvelles molécules anti-infectieuses sur microarray : applications à Pseudomonas aeruginosa : Validation and screening of new anti-infective molecules on microarray : applications to Pseudomonas aeruginosa. [Doctoral Dissertation]. Lyon; 2016. Available from: http://www.theses.fr/2016LYSEC018

University of Michigan

29. Liu, Yuwei. Surface Studies on the Structure and Functionality of Bioactive Materials.

Degree: PhD, Chemistry, 2014, University of Michigan

URL: http://hdl.handle.net/2027.42/109042

► Bioactive materials are critical in many applications in the fields ranging from antibiofouling coatings to tissue engineering to biosensing devices. The construction of bioactive materials… (more)

▼ Bioactive materials are critical in many applications in the fields ranging from antibiofouling coatings to tissue engineering to biosensing devices. The construction of bioactive materials generally involves dispersion of bioactive components such as biocides, peptides or proteins in organic or inorganic matrices. However, when these bioactive components are incorporated to such matrices, their functionalities may be significantly hindered. As a result, it has spurred great attention seeking guidelines for future rational design and development of bioactive materials with optimal functionalities. This thesis proposes optimization strategies by investigating interfacial molecular interactions of various bioactive materials in situ using surface specific spectroscopies and correlating the structural and orientational information to their activity. Firstly, two biocide-incorporated antifouling polymeric materials are characterized using sum frequency generations vibrational spectroscopy at solid/liquid interfaces. The interaction mechanism is unraveled by varying both biocidal and the polymer components of the system. The structure of the alkyl chains from the biocidal components is determined and by comparing to their antifouling performance, it is concluded that the protruding-out state offers the best antifouling activity. Factors that affect the structure of the biocidal components are examined. Secondly, antimicrobial peptides are immobilized to various surface platforms. The immobilization processes are thoroughly monitored and the final immobilized peptide structures and orientations are determined using spectroscopic technique. The immobilization environment, the location of the attachment point and the peptide sequence all play important roles in determining the immobilization process and the final state of the immobilized peptides. Finally, a model enzyme is immobilized to solid surfaces and characterized by using combined spectroscopic measurements. These results are further correlated to the enzymatic activities and confirmed by molecular dynamics simulations performed by our collaborators. It is concluded that the surface properties, the location of the attachment point, the secondary structure of the attachment point are factors influencing the immobilized enzyme structure and further determining the activity and stability of the immobilized enzyme. In all, this thesis provides systematic understanding how structural differences are related to the difference in the functionality of the bioactive materials under study based on which the optimization designing rules are proposed. Advisors/Committee Members: Chen, Zhan (committee member), Kuroda, Kenichi (committee member), McNeil, Anne Jennifer (committee member), Marsh, E Neil G. (committee member).

Subjects/Keywords: Surface Analysis; Sum Frequency Generation; Antibiofouling Coatings; Antimicrobial Peptide Immobilization; Enzyme Immobilization; Structure-activity Relationship; Chemistry; Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2014). Surface Studies on the Structure and Functionality of Bioactive Materials. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/109042

Chicago Manual of Style (16^th Edition):

Liu, Yuwei. “Surface Studies on the Structure and Functionality of Bioactive Materials.” 2014. Doctoral Dissertation, University of Michigan. Accessed June 17, 2019. http://hdl.handle.net/2027.42/109042.

MLA Handbook (7^th Edition):

Liu, Yuwei. “Surface Studies on the Structure and Functionality of Bioactive Materials.” 2014. Web. 17 Jun 2019.

Vancouver:

Liu Y. Surface Studies on the Structure and Functionality of Bioactive Materials. [Internet] [Doctoral dissertation]. University of Michigan; 2014. [cited 2019 Jun 17]. Available from: http://hdl.handle.net/2027.42/109042.

Council of Science Editors:

Liu Y. Surface Studies on the Structure and Functionality of Bioactive Materials. [Doctoral Dissertation]. University of Michigan; 2014. Available from: http://hdl.handle.net/2027.42/109042

30. Kumar, Lalit. Production purification immobilization and application of a bacterial xylanase; -.

Degree: Bio chemistry, 2014, Kurukshetra University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/39901

None

References givan

Advisors/Committee Members: Garg, Neelam and Gupta, V K.

Subjects/Keywords: Bacterial xylanase; Production; Purification immobilization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kumar, L. (2014). Production purification immobilization and application of a bacterial xylanase; -. (Thesis). Kurukshetra University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/39901

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kumar, Lalit. “Production purification immobilization and application of a bacterial xylanase; -.” 2014. Thesis, Kurukshetra University. Accessed June 17, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/39901.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kumar, Lalit. “Production purification immobilization and application of a bacterial xylanase; -.” 2014. Web. 17 Jun 2019.

Vancouver:

Kumar L. Production purification immobilization and application of a bacterial xylanase; -. [Internet] [Thesis]. Kurukshetra University; 2014. [cited 2019 Jun 17]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/39901.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kumar L. Production purification immobilization and application of a bacterial xylanase; -. [Thesis]. Kurukshetra University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/39901

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations