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University of Illinois – Chicago
1.
Hill, Brittaney.
Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.
Degree: 2017, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/22043
► Objective: To determine if caregiver race/ethnicity impacts their willingness to accept passive immobilization and to determine if a written and pictorial description of the technique…
(more)
▼ Objective: To determine if caregiver race/ethnicity impacts their willingness to accept passive
immobilization and to determine if a written and pictorial description of the technique will make the caregivers more likely to accept the technique.
Methods: Caregivers of patients age 1-16 were recruited in the Post Graduate Pediatric Dentistry Clinic at the University of Illinois at Chicago College of Dentistry. If caregivers opted to participate, they completed a questionnaire, reviewed a pictorial and written description of the technique, and then completed another questionnaire. The data was analyzed in SPSS using Kruskal Wallis ANOVA and Wilcoxon Signed Ranks with a p-value of 0.05.
Results: A total of 284 subjects, who met the inclusion criteria, were entered and consented for this study. Eighteen were excluded from data analysis. Therefore, the sample size of 266 caregivers were used for statistical analysis.
Hypothesis 1: The null hypothesis that there is no difference in the caregiver's willingness to accept passive
immobilization based upon race was rejected using Kruskal Wallis ANOVA at p=0.05. Based upon initial attitudes, subjects consented to passive
immobilization at the following percentages: Hispanic (84%), African American (66%), Asian (50%) and Caucasian (24%).
Hypothesis 2: The null hypothesis that there is no difference in willingness to accept passive
immobilization after reviewing written and pictorial description was rejected using Wilcoxon Signed Ranks at p=0.05.
Conclusions: We conclude that caregiver race/ethnicity does impact their willingness to accept passive
immobilization in our patient population. We also conclude that caregivers are more likely to accept the use of passive
immobilization after they are given more information about the technique.
Advisors/Committee Members: Fadavi, Shahrbanoo (advisor), LeHew, Charles (committee member), Rada, Robert (committee member), Fadavi, Shahrbanoo (chair).
Subjects/Keywords: Passive Immobilization
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APA (6th Edition):
Hill, B. (2017). Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/22043
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hill, Brittaney. “Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.” 2017. Thesis, University of Illinois – Chicago. Accessed April 14, 2021.
http://hdl.handle.net/10027/22043.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hill, Brittaney. “Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment.” 2017. Web. 14 Apr 2021.
Vancouver:
Hill B. Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10027/22043.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hill B. Caregiver’s Race/Ethnicity on Acceptance of Passive Immobilization for their Child’s Dental Treatment. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/22043
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta
2.
Ikeda, Yuko.
CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES.
Degree: PhD, Department of Agricultural, Food, and Nutritional
Science, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/2r36tz97r
► Immobilization has the potential to make enzyme utilization more cost efficient, but reduced enzymatic activity makes it unfeasible. The purpose of this study was to…
(more)
▼ Immobilization has the potential to make enzyme
utilization more cost efficient, but reduced enzymatic activity
makes it unfeasible. The purpose of this study was to develop
immobilized cellulase systems on silica supports that retain high
enzymatic activity. Two of the four systems created resulted in
better-than-expected productivity during hydrolysis and make good
candidates for further research. The immobilized systems on fumed
non-porous silica retained enzyme activity equivalent to free
cellulases when hydrolyzing crystalline cellulose. However, sugar
composition analysis indicated that immobilization decreased
cellobiose conversion to glucose, resulting in substantial
cellobiose production. More research is required to understand the
mechanism at work. The immobilized cellulases obtained wider pH
stability, but displayed decreased thermal and ionic stability.
Storage stability was unchanged. One major finding was increased
enzyme reusability, with activity remaining at 30% after nine uses.
When lignocellulose biomass was used for hydrolysis, production was
substantially lower than when using free cellulases. It is
hypothesized that the immobilized cellulases had difficulty
hydrolyzing the amorphous regions of the lignocellulose. Although
further optimization is necessary, the immobilized cellulase
systems developed in this study contribute greatly to increasing
the commercial viability of hydrolysis and lignocellulose
pretreatment.
Subjects/Keywords: Biorefinery; Cellulases; Immobilization
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APA (6th Edition):
Ikeda, Y. (2013). CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2r36tz97r
Chicago Manual of Style (16th Edition):
Ikeda, Yuko. “CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES.” 2013. Doctoral Dissertation, University of Alberta. Accessed April 14, 2021.
https://era.library.ualberta.ca/files/2r36tz97r.
MLA Handbook (7th Edition):
Ikeda, Yuko. “CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES.” 2013. Web. 14 Apr 2021.
Vancouver:
Ikeda Y. CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2021 Apr 14].
Available from: https://era.library.ualberta.ca/files/2r36tz97r.
Council of Science Editors:
Ikeda Y. CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC
ACTIVITIES. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/2r36tz97r

Universiteit Utrecht
3.
Tjong, C.T.F.
Immobilization strategies for peptide microarrrays.
Degree: 2012, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/256506
► Peptide microarrays have been developed over the last decade into a technology that can profile biomolecules. It has proven to be a versatile tool for…
(more)
▼ Peptide microarrays have been developed over the last decade into a technology that can profile biomolecules. It has proven to be a versatile tool for epitope-mapping, substrate profiling and probing antigen-antibody, protein-protein and protein-ligand interactions, which all can lead or have led to the discovery of new drugs and drug targets. Microarrays are convenient to use because only miniscule quantities are needed for screening a high number of substances. Peptides used in peptide microarrays are synthesized in such a way that they can bind via either a covalent or non-covalent bond to the microarray surface. These peptides often need a special functionality, but there are also strategies that make use of the occurring moieties in the peptide to achieve
immobilization. This thesis gives a short summary of the development of peptide microarrays and discusses the strategies that have been developed for the
immobilization of peptides on glass microscopic slides.
Advisors/Committee Members: Kruijtzer, J.A.W., Rijkers, D.T.S..
Subjects/Keywords: Immobilization strategies; Peptide Microarrays; Peptides
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tjong, C. T. F. (2012). Immobilization strategies for peptide microarrrays. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/256506
Chicago Manual of Style (16th Edition):
Tjong, C T F. “Immobilization strategies for peptide microarrrays.” 2012. Masters Thesis, Universiteit Utrecht. Accessed April 14, 2021.
http://dspace.library.uu.nl:8080/handle/1874/256506.
MLA Handbook (7th Edition):
Tjong, C T F. “Immobilization strategies for peptide microarrrays.” 2012. Web. 14 Apr 2021.
Vancouver:
Tjong CTF. Immobilization strategies for peptide microarrrays. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Apr 14].
Available from: http://dspace.library.uu.nl:8080/handle/1874/256506.
Council of Science Editors:
Tjong CTF. Immobilization strategies for peptide microarrrays. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/256506
4.
Mathew,P T.
Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.
Degree: Marine Science;, 1989, Cochin University of Science and Technology
URL: http://dyuthi.cusat.ac.in/purl/1224
Subjects/Keywords: Immobilization; Purification
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APA ·
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APA (6th Edition):
T, M. (1989). Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/1224
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
T, Mathew,P. “Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.” 1989. Thesis, Cochin University of Science and Technology. Accessed April 14, 2021.
http://dyuthi.cusat.ac.in/purl/1224.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
T, Mathew,P. “Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish.” 1989. Web. 14 Apr 2021.
Vancouver:
T M. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. [Internet] [Thesis]. Cochin University of Science and Technology; 1989. [cited 2021 Apr 14].
Available from: http://dyuthi.cusat.ac.in/purl/1224.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
T M. Purification and Characterization of Glucose 6-Phosphate Dehydrogenase in Fish. [Thesis]. Cochin University of Science and Technology; 1989. Available from: http://dyuthi.cusat.ac.in/purl/1224
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University
5.
Lata, James.
Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.
Degree: PhD, Biomedical Engineering, 2015, Cornell University
URL: http://hdl.handle.net/1813/40952
► The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has…
(more)
▼ The primary advances in creating a point-of-care testing (PoCT) diagnostic platform involve miniaturizing assays based on recognition of biomarker antigens with antibodies. This approach has inherent issues with time-sensitive pathologies because of the inherently slow speed of antibodyantigen interactions. Such an obstacle can be overcome through use of faster enzymatic reactions. In this work, we focused on creating an enzymatic based PoCT diagnostic platform suitable for timesensitive pathologies, in particular addressing stroke. We hypothesize that by immobilizing enzymes to nanoparticles (NP) the enzymes will be stabilized and confined to a smaller volume, therefore increasing the efficiency of sequential enzymatic reactions needed to detect biomarkers at physiological and pathological levels in human serum and transduce that detection into a luminescent signal. Development of this platform involved several key steps, including immobilizing functional enzymes on inorganic surfaces and developing microfluidic systems with microliter-volume reactions. Because the use of immobilized enzymes in hybrid organic-inorganic devices is in a nascent stage of development, it is also essential to work out fundamental principles of how the inorganic scaffolds might affect the activities of the tethered enzymes. Through biomimicry of the sperm flagellum, enzymes were designed to include a His-tag to bind with Ni-NTA groups on an inorganic surface in a precise orientation. This approach yielded higher specific activity than did attachment of the same enzymes through adsorption or chemically-specific binding (e.g. amine-carboxyl) techniques. While the orientation had dramatic impacts on the enzyme function, the surface size/curvature to which it is bound also influenced the enzyme function. Prior literature on this relationship was largely anecdotal. To identify generalizable truths regarding the iii effects of the surface size and curvature on enzyme function, we used three enzymes, each representing a different class of enzyme, at maximal enzyme loading and in monolayers on gold NPs ranging in size from 5 to 50 nm in diameter. We found that larger NPs had increased enzyme multilayer formations and decreased kcat and specific activity by 2.8 to 8.3 fold, depending on the enzyme. Contrary to other reports and suggesting an advantage for oriented
immobilization, we did not see effects of size on the same enzymes when tethered in monolayers. Tethering to NPs has immediate application in medical diagnosis of time-sensitive pathologies due to enzymes' rapid catalysis versus slow antibody interactions. Neuron specific enolase (NSE) is a biomarker for stroke, which we detected through the sequential reaction of tethered luciferase (Luc) and pyruvate kinase (PK), yielding a luminescent signal proportional to the concentration of enolase in our samples. In both rat stroke models and human patient samples, we found this diagnostic platform gave accurate results in 10 minutes compared to typical 4 hour ELISA tests, yet maintained a high…
Advisors/Committee Members: Travis,Alexander J. (chair), Erickson,David (committee member), Jin,Moonsoo (committee member).
Subjects/Keywords: Protein Engineering; Diagnostic; Enzyme Immobilization
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lata, J. (2015). Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/40952
Chicago Manual of Style (16th Edition):
Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.” 2015. Doctoral Dissertation, Cornell University. Accessed April 14, 2021.
http://hdl.handle.net/1813/40952.
MLA Handbook (7th Edition):
Lata, James. “Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies.” 2015. Web. 14 Apr 2021.
Vancouver:
Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1813/40952.
Council of Science Editors:
Lata J. Engineering A Rapid Point-Of-Care Diagnostic Platform Utilizing Tethered Enzyme Technology For Time-Sensitive Pathologies. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40952

Penn State University
6.
Gaddes, David Edwin.
Remote Calorimetric Biosensing For Clinical Diagnostic Applications.
Degree: 2016, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/12544deg5098
► The medical community increasingly relies on quantification of biomarkers in blood, urine, and saliva to monitor treatments and screen for diseases. While glucose meters have…
(more)
▼ The medical community increasingly relies on quantification of biomarkers in blood, urine, and saliva to monitor treatments and screen for diseases. While glucose meters have been around since the 1980s only two biosensors have gained success in commercial applications, the glucose meter and the pregnancy test. The majority of biomarkers are quantified using a benchtop analytical system developed specifically for one biomarker. We developed a portable, calorimetric biosensor, which has the capability of quantifying a large variety of biomarkers. Specifically, our goal was to develop this biosensor to quantify creatinine in human urine samples. Further, we sought to develop a sensitive and robust calorimeter, in which the enzyme containing cartridge could be quickly removed and replaced. To achieve this, we implemented a highly sensitive Y-cut quartz crystal resonator as the sensing mechanism in this calorimetric biosensor. The high-temperature sensitivity of the quartz resonator enables a sensitive calorimetric system despite the separation between the fluid of interest and the sensor through 100 μm gap of air. Moreover, to further improve the calorimetric performance of this device we implemented a second quartz resonator, which acts as a reference sensor to eliminate spurious noise from thermal fluctuations. Additionally, we evaluate three enzyme
immobilization techniques: glutaraldehyde crosslinking, electrostatic layer-by-layer
immobilization, and alginate entrapment using both a fluorescent assay and the calorimetric biosensor. This work is concluded by quantifying the concentration of creatinine in human urine samples. The creatinine concentrations determined using the biosensors were compared against those determined through an HPLC technique. This Ph.D. work has successfully demonstrated the development of a calorimetric biosensor, specifically for the quantification of creatinine in human urine samples.
Advisors/Committee Members: Srinivas Tadgiadapa, Dissertation Advisor/Co-Advisor.
Subjects/Keywords: Biosensor; calorimetry; microfabrication; enzyme immobilization
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❌
APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Gaddes, D. E. (2016). Remote Calorimetric Biosensing For Clinical Diagnostic Applications. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/12544deg5098
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Thesis, Penn State University. Accessed April 14, 2021.
https://submit-etda.libraries.psu.edu/catalog/12544deg5098.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gaddes, David Edwin. “Remote Calorimetric Biosensing For Clinical Diagnostic Applications.” 2016. Web. 14 Apr 2021.
Vancouver:
Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Internet] [Thesis]. Penn State University; 2016. [cited 2021 Apr 14].
Available from: https://submit-etda.libraries.psu.edu/catalog/12544deg5098.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gaddes DE. Remote Calorimetric Biosensing For Clinical Diagnostic Applications. [Thesis]. Penn State University; 2016. Available from: https://submit-etda.libraries.psu.edu/catalog/12544deg5098
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Nova
7.
Couto, Cláudia Alexandra Maia do.
Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.
Degree: 2012, Universidade Nova
URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145
► Thesis for the master degree in Structural and Functional Biochemistry
The work presented here had the objective of using gold nanoparticles (AuNPs) functionalized with mercaptoundecanoic…
(more)
▼ Thesis for the master degree in Structural and Functional Biochemistry
The work presented here had the objective of using gold nanoparticles (AuNPs) functionalized with mercaptoundecanoic acid (MUA), or the peptide CALNN, to develop bionanoconjugates (BNCs) with the enzyme Rhus vernicifera (Rv) laccase. Laccases are multi copper oxidases able to catalyze the oxidation of a variety of phenolic compounds with the reduction of molecular oxygen to water, and conjugating this enzyme with AuNPs could potentially contribute to enhance its catalytic activity.
Spectroscopic, electrophoretic, kinetic, and computer modelling studies were carried out in order to characterize the enzyme structurally and investigate its stability and activity when conjugated with AuNPs.
The studies performed revealed that Rv laccase is a monomer with 5.65 ± 0.83 nm in diameter and presents an optimal activity at pH 7.5, when syringaldazine was used as substrate.
Laccase was successfully adsorbed to AuNP-CALNN but not to AuNP-MUA. BNCs with laccase and AuNP-CALNN remained active in the experimental conditions tested at pH from 6 – 8.5.
Laccase followed a Michaelis-Menten kinetic model using syringaldazine as a substrate, in the pH range 6 – 8.5. Parallel studies with free laccase were conducted to evaluate the influence of AuNP-CALNN on laccase activity. These kinetic studies were performed by following substrate consumption and product formation. The kinetic results with less error were obtained when the reaction of product formation was followed. Therefore, the results for product formation were considered to be more reliable than the results obtained for substrate consumption.
The results for product formation revealed that the catalytic efficiency of BNCs was more than 50% higher at pH 7, almost 100% higher at pH 7.5 and 30% higher at pH 8 in comparison with free laccase. At pH 6 there was a decrease of approximately 38% in the catalytic efficiency. The catalytic efficiency was similar in BNCs and free laccase at pH 6.5 and 8.5. In the pH range 6.5 to 8.5, AuNP-CALNN proved beneficial by either maintaining or increasing the catalytic activity of laccase. Using AuNP-CALNN to immobilize laccase might allow decreasing industrial costs, recycling the enzyme and improving the outcome of processes usually achieved with free laccase, such as delignification, wine clarification, textile dye bleaching or waste water detoxification.
Advisors/Committee Members: Franco, Ricardo.
Subjects/Keywords: Gold nanoparticles; Laccase; Bionanoconjugates; Immobilization
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Couto, C. A. M. d. (2012). Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Couto, Cláudia Alexandra Maia do. “Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.” 2012. Thesis, Universidade Nova. Accessed April 14, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Couto, Cláudia Alexandra Maia do. “Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies.” 2012. Web. 14 Apr 2021.
Vancouver:
Couto CAMd. Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. [Internet] [Thesis]. Universidade Nova; 2012. [cited 2021 Apr 14].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Couto CAMd. Active bionanoconjugates of laccase and gold nanoparticles: kinetic and structural studies. [Thesis]. Universidade Nova; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/8145
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
8.
Fuchs, Brian K.
Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.
Degree: MS, Chemical Engineering, 2015, Oregon State University
URL: http://hdl.handle.net/1957/56327
► Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20%…
(more)
▼ Methane is a flammable gas that is the main component of natural gas. It is a highly potent greenhouse gas, and accounts for about 20% of greenhouse gas emissions. Methane is routinely flared in many industrial processes without harnessing any of its energy. The environmental impact and wasted energy potential make it highly desirable to find an economically feasible process to use this methane.
One possibility is to convert methane into liquid fuels for transportation and energy generation. Current technologies to convert methane gas to liquid fuels (GTL) are complex, and the facilities are only economical at huge scales. Methane gas is very difficult to transport and store, so GTL plants must be located at the source of the methane, typically at large petroleum fields or refineries.
Biological conversion of methane to liquid fuels is an attractive alternative to traditional GTL processes, as microbial oxidation of methane can produce liquid fuels (e.g. methanol) at ambient temperatures and pressures. When biological organisms are combined with microfluidic technologies, which provide enhanced mass and heat transfer along with a high degree of process control, a very efficient conversion process can be attained at much smaller scales. A further advantage of microfluidics is that the reactors are inherently modular, allowing them to be adapted to practically any required size. This enables the economical conversion of small or remote methane streams to liquid fuels.
In this thesis, techniques are described for the
immobilization of Methylosinus trichosporium OB3b in a biolamina-plate microreactor (BLP) for conversion of methane to methanol. Effective
immobilization requires that the cells remain viable and immobile in the reactor, and that the encapsulation medium is stable and does not degrade during reactor operation. Calcium alginate gels were identified as an ideal
immobilization medium, as they are inexpensive, non-toxic, and widely used for the
immobilization of cells. Three main requirements must be met in
immobilization of cells in the alginate: gel cohesion, gel adhesion, and cell viability. The alginate gel must remain cohesive throughout the entire reactor process, without substantial swelling, disintegration or degradation. The alginate must also adhere stably to the reactor surface, to prevent sloughing which may cause clogging and loss of biological activity. The immobilized cells also must remain metabolically active over the duration of the reactor run. Stable, thin (300-μm) calcium alginate films were achieved by combining an “internal gelation” process to uniformly cross-link the hydrogel, electrostatic adhesion of alginate gel on stainless steel reactor plates modified with aminopropyl-trimethoxysilane (APTMS), and buffering using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) to minimize chelation of stabilizing calcium ions from the gel. The alginate-encapsulated OB3b cells retain viability and metabolic activity in these films, although their metabolism of methane to methanol…
Advisors/Committee Members: Schilke, Karl F. (advisor), Remcho, Vincent T. (committee member).
Subjects/Keywords: cell immobilization; Methanotrophs – Biotechnology
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fuchs, B. K. (2015). Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/56327
Chicago Manual of Style (16th Edition):
Fuchs, Brian K. “Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.” 2015. Masters Thesis, Oregon State University. Accessed April 14, 2021.
http://hdl.handle.net/1957/56327.
MLA Handbook (7th Edition):
Fuchs, Brian K. “Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol.” 2015. Web. 14 Apr 2021.
Vancouver:
Fuchs BK. Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. [Internet] [Masters thesis]. Oregon State University; 2015. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1957/56327.
Council of Science Editors:
Fuchs BK. Immobilization of Methylosinus trichosporium OB3b in a Biolamina Plate Reactor for the Oxidation of Methane to Methanol. [Masters Thesis]. Oregon State University; 2015. Available from: http://hdl.handle.net/1957/56327

McMaster University
9.
Shakeri, Amid.
Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics.
Degree: PhD, 2020, McMaster University
URL: http://hdl.handle.net/11375/26035
► Biofunctional interfaces capable of anchoring biomolecules and nanoparticles of interest onto a platform are the key components of many biomedical assays, clinical pathologies, as well…
(more)
▼ Biofunctional interfaces capable of anchoring biomolecules and nanoparticles of interest onto a platform are the key components of many biomedical assays, clinical pathologies, as well as antibacterial and antiviral surfaces. In an ideal biofunctional surface, bio-entities and particles are covalently immobilized on a substrate in order to provide robustness and long-term stability. Nonetheless, most of the reported covalent immobilization strategies incorporate complex wet-chemical steps and long incubation times hindering their implementation for mass production and commercialization. Another essential factor in the biointerface preparation, specially with regard to biosensors and diagnostic applications, is utilization of an efficient and durable blocking agent that can inhibit non-specific adsorption of biomolecules thereby enhancing the sensitivity of sensors by diminishing the level of background noise. Many of the commonly used blocking agents lack proper prevention of non-specific adsorption in complex fluids. In addition, most of these agents are physically attached to surfaces making them unreliable for long-term usage in harsh environments (i.e. where shear stresses above 50 dyn/cm2 or strong washing buffers are involved).
This thesis presents novel and versatile strategies to covalently immobilize nanoparticles and biomolecules on substrates. The new surface coating techniques are first implemented for robust attachment of TiO2 nanoparticles onto ceramic tiles providing self-cleaning properties. Further, we utilize similar strategies to covalently immobilize proteins and culture cells in microfluidic channels either as a full surface coating or as micropatterns of interest. The new strategies allow us to obtain adhesion of ~ 400 cells/mm2 in microfluidic channels after only 1-day incubation, which is not achievable by the conventional methods. Moreover, we show the possibility of covalently micropatterning of biomolecules on lubricant-infused surfaces (LISs) so as to attain a new class of biofunctional LISs. By integration of these surfaces into a biosensing platform, we are able to detect interleukin 6 (IL-6) in a complex biofluid of human whole plasma with a limit of detection (LOD) of 0.5 pg.mL-1. This LOD is significantly lower than the smallest reported IL-6 LOD in plasma, 23 pg mL-1, using a complex electrochemical system. The higher sensitivity of our developed biosensor can be attributed to the distinguish capability of biofunctional LISs in preventing non-specific adhesion of biomolecules compared to other blocking agents.
Thesis
Doctor of Philosophy (PhD)
The key goal of this thesis is to provide new strategies for preparation of robust and durable biointerfaces that could be employed for many biomedical devices such as self-cleaning coatings, microfluidics, point-of-care diagnostics, biomedical assays, and biosensors in order to enhance their efficiency, sensitivity, and precision. The introduced surface biofunctionalization methods are straightforward to use and do not require…
Advisors/Committee Members: Didar, Tohid, Mechanical Engineering.
Subjects/Keywords: Biofunctionalization; Biosensors; Microfluidics; biomolecule immobilization
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MLA ·
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APA (6th Edition):
Shakeri, A. (2020). Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics. (Doctoral Dissertation). McMaster University. Retrieved from http://hdl.handle.net/11375/26035
Chicago Manual of Style (16th Edition):
Shakeri, Amid. “Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics.” 2020. Doctoral Dissertation, McMaster University. Accessed April 14, 2021.
http://hdl.handle.net/11375/26035.
MLA Handbook (7th Edition):
Shakeri, Amid. “Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics.” 2020. Web. 14 Apr 2021.
Vancouver:
Shakeri A. Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics. [Internet] [Doctoral dissertation]. McMaster University; 2020. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/11375/26035.
Council of Science Editors:
Shakeri A. Development of Robust Biofunctional Interfaces for Applications in Selfcleaning Surfaces, Lab-Ona-Chip Systems, and Diagnostics. [Doctoral Dissertation]. McMaster University; 2020. Available from: http://hdl.handle.net/11375/26035

Louisiana State University
10.
Calixte, Nyote J.
From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.
Degree: PhD, Chemistry, 2014, Louisiana State University
URL: etd-08272014-211241
;
https://digitalcommons.lsu.edu/gradschool_dissertations/924
► The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of…
(more)
▼ The process of immobilizing enzymes onto solid supports for bioreactions has some compelling advantages compared to their solution-based counterpart including the facile separation of enzyme from products, elimination of enzyme autodigestion, and increased enzyme stability and activity. We report in this work, the immobilization of λ-exonuclease onto poly(methylmethacrylate) (PMMA) micro- and nano-pillars populated within a fluidic devices for the micro and nanoscale on-chip digestion of double-stranded DNA. Enzyme immobilization in both studies was successfully accomplished using 3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling to carboxylic acid functionalized PMMA micropillars. Our micro-scale results suggest that the reaction efficiency for the catalysis of dsDNA digestion using λ-exonuclease, including its processivity and reaction rate, were higher when the enzyme was attached to a solid support compared to the free solution digestion. We obtained a clipping rate of 1.0 x 103 nucleotides s-1 for the digestion of λ-DNA (48.5 kbp) by λ-exonuclease. We suggest that the kinetic behavior of this solid-phase reactor could be described by a fractal Michaelis-Menten. Preliminary nano-scale λ-Exo immobilization experiments reveal potential enzymatic activity changes as observed in reduced digestion rates (~303 nucleotides s-1). Further studies will deduce reasoning for these observed differences. Simulation of the nanofluidic reactors reveal kinetic behavior to be mass transport limited, a result not expected due to the reduction in reactor dimensions. Nonetheless, the results from these studies work will have important ramifications in new single-molecule DNA sequencing strategies that employ free mononucleotide identification. As a step towards this goal, an investigation of the dynamics of DNA in these irregularly shaped structures has been performed.
Subjects/Keywords: kinetics; microscale; nanoscale; immobilization; enzyme
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APA (6th Edition):
Calixte, N. J. (2014). From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924
Chicago Manual of Style (16th Edition):
Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Doctoral Dissertation, Louisiana State University. Accessed April 14, 2021.
etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.
MLA Handbook (7th Edition):
Calixte, Nyote J. “From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly.” 2014. Web. 14 Apr 2021.
Vancouver:
Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Internet] [Doctoral dissertation]. Louisiana State University; 2014. [cited 2021 Apr 14].
Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924.
Council of Science Editors:
Calixte NJ. From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly. [Doctoral Dissertation]. Louisiana State University; 2014. Available from: etd-08272014-211241 ; https://digitalcommons.lsu.edu/gradschool_dissertations/924

Louisiana State University
11.
Mai, Anthony Quan Quoc.
Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme.
Degree: PhD, Biology and Biomimetic Materials, 2021, Louisiana State University
URL: https://digitalcommons.lsu.edu/gradschool_dissertations/5464
► Urease enzyme was crystallized almost a century ago, and to this day its intrinsic stability is not ideal for everyday applications. This work introduces…
(more)
▼ Urease enzyme was crystallized almost a century ago, and to this day its intrinsic stability is not ideal for everyday applications. This work introduces a new process by which a naturally encapsulated material, watermelon seed powder (WMSP), is characterized for its stability and activity. WMSP enzymatic activity has been measured for over a year at various storage conditions—exposed to ambient atmosphere for a year, WMSP retained above 90% activity. In aqueous conditions, the enzyme maintained above 60% activity after two months; with the addition of a preservative that number stays at about 90%. There is a pH shift of the maximum activity at 8.1 pH in addition to a broadening of the activity curve allowing for a wider range of reactions.
In Chapter 3, immobilization techniques were employed on these powders, enhancing their usability and in turn presented some interesting dynamics. One of the techniques physically encapsulated the WMSP inside a porous resin, essentially creating a urea-ammonia exchange resin. These particles were subject to 10 cycles of urea hydrolysis, without any change in the urea-urease clock reaction. The agar immobilized WMSPs exhibited a behavior called quorum sensing. That is a biological communication process by which bacteria express certain genes when a “quorum” or sufficient number are in proximity of each other. With certain parameters, the particles will not clock individually, but when in a cluster of particles, the autocatalysis occurs. This property was then coupled with a hydrogel polymerization that gels in basic conditions. The WMSP particles in principle are biofilm generating analogues. Lastly, Chapter 4 presents an application of WMSP as a base generator for several adhesive formulations and outlines some potential future uses of WMSP.
Subjects/Keywords: Polymers; Urease; Watermelon Seeds; Immobilization
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APA (6th Edition):
Mai, A. Q. Q. (2021). Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme. (Doctoral Dissertation). Louisiana State University. Retrieved from https://digitalcommons.lsu.edu/gradschool_dissertations/5464
Chicago Manual of Style (16th Edition):
Mai, Anthony Quan Quoc. “Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme.” 2021. Doctoral Dissertation, Louisiana State University. Accessed April 14, 2021.
https://digitalcommons.lsu.edu/gradschool_dissertations/5464.
MLA Handbook (7th Edition):
Mai, Anthony Quan Quoc. “Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme.” 2021. Web. 14 Apr 2021.
Vancouver:
Mai AQQ. Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme. [Internet] [Doctoral dissertation]. Louisiana State University; 2021. [cited 2021 Apr 14].
Available from: https://digitalcommons.lsu.edu/gradschool_dissertations/5464.
Council of Science Editors:
Mai AQQ. Characterization, Immobilization, and Polymer Related Applications of Watermelon Seed Powder, a Practical Source of Urease Enzyme. [Doctoral Dissertation]. Louisiana State University; 2021. Available from: https://digitalcommons.lsu.edu/gradschool_dissertations/5464
12.
GIRALDELLI, MARILENE A.
Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos.
Degree: 1990, Brazil
URL: http://repositorio.ipen.br:8080/xmlui/handle/123456789/10236
Made available in DSpace on 2014-10-09T12:36:22Z (GMT). No. of bitstreams: 0
Made available in DSpace on 2014-10-09T13:59:30Z (GMT). No. of bitstreams: 1 03996.pdf: 3342659 bytes, checksum: 712f3dbe2d259d924e05243e956d4e94 (MD5)
Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
Advisors/Committee Members: Yasuko Tesuka.
Subjects/Keywords: cements; immobilization; radioactive wastes; immobilization
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APA (6th Edition):
GIRALDELLI, M. A. (1990). Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos. (Masters Thesis). Brazil. Retrieved from http://repositorio.ipen.br:8080/xmlui/handle/123456789/10236
Chicago Manual of Style (16th Edition):
GIRALDELLI, MARILENE A. “Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos.” 1990. Masters Thesis, Brazil. Accessed April 14, 2021.
http://repositorio.ipen.br:8080/xmlui/handle/123456789/10236.
MLA Handbook (7th Edition):
GIRALDELLI, MARILENE A. “Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos.” 1990. Web. 14 Apr 2021.
Vancouver:
GIRALDELLI MA. Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos. [Internet] [Masters thesis]. Brazil; 1990. [cited 2021 Apr 14].
Available from: http://repositorio.ipen.br:8080/xmlui/handle/123456789/10236.
Council of Science Editors:
GIRALDELLI MA. Alguns aspectos sobre a utilizacao de cimento Portland como matriz para imobilizacao de rejeitos radioativos. [Masters Thesis]. Brazil; 1990. Available from: http://repositorio.ipen.br:8080/xmlui/handle/123456789/10236

University of Alberta
13.
Hass, Michael J.
Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations.
Degree: MS, Department of Chemistry, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/n296x015t
► A method was developed to immobilize and reuse a catalyst for the asymmetric cycloisomerization of enynes. Immobilization is achieved through use of metal containing monomers,…
(more)
▼ A method was developed to immobilize and reuse a
catalyst for the asymmetric cycloisomerization of enynes.
Immobilization is achieved through use of metal containing
monomers, which are reacted with a cycloolefin via an alternating
ring-opening metathesis polymerization, assembling a
three-dimensional catalyst-organic framework. For example,
[RhCl((R)-5,5'-dinorimido BINAP)]2 was copolymerized with
ciscyclooctene using trans-RuCl2(CHPh)(PCy3)2 as a catalyst. After
supporting the resulting framework on barium sulphate, silver(I)
hexafluoroantimonate was added in the presence of enyne substrates
to generate cationic rhodium(I) sites. These catalytic rhodium(I)
sites catalyzed the asymmetric cycloisomerization of enynes, and
afforded a maximum of 620 turnovers and >99% ee over the
course of six runs. This catalyst was also used for batch reactions
with turnovers as high as 800. The maximum number of turnovers
reported in literature for a homogeneous non-tandem
cycloisomerization reaction is ten.
Subjects/Keywords: cycloisomerization; rhodium; catalyst immobilization; catalysis; enantioselective
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Hass, M. J. (2012). Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/n296x015t
Chicago Manual of Style (16th Edition):
Hass, Michael J. “Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations.” 2012. Masters Thesis, University of Alberta. Accessed April 14, 2021.
https://era.library.ualberta.ca/files/n296x015t.
MLA Handbook (7th Edition):
Hass, Michael J. “Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations.” 2012. Web. 14 Apr 2021.
Vancouver:
Hass MJ. Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Apr 14].
Available from: https://era.library.ualberta.ca/files/n296x015t.
Council of Science Editors:
Hass MJ. Reusable Ru and Rh catalysts for ester hydrogenations and
enyne cycloisomerizations. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/n296x015t
14.
Ogorzalek, Tadeusz L.
Examining the Behavior of Surface Tethered Enzymes.
Degree: PhD, Chemical Biology, 2015, University of Michigan
URL: http://hdl.handle.net/2027.42/111541
► Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how…
(more)
▼ Surface-immobilized enzymes are important for a wide range of technological applications, including industrial catalysis, drug delivery, medical diagnosis and biosensors. However, our understanding of how enzymes and proteins interact with abiological surfaces on the molecular level remains extremely limited. We have compared the structure, activity and thermal stability of beta-galactosidase variants attached to a chemically well-defined self assembled monolayer (SAM) surface. Maleimide-terminated ethylene glycol linkers were used to attach beta-galactosidase through a unique cysteinyl residue. These maleimide-terminated linkers were mixed with ethylene glycol linkers terminated with different chemical moieties to engineer surfaces with varying hydrophobicity and electrostatic charge. In collaboration with the Chen Lab, we used SFG and ATR-FTIR to experimentally measure the orientation of the surface tethered enzyme. In collaboration with the Brooks Lab, we conducted coarse grain model simulations to examine the atomic level interactions between the protein and the surface. Through coarse grain modelling, it was shown that the increased range of motion allowed to an enzyme tethered to a flexible loop region increased the number of protein surface interactions relative to the interactions experienced by an enzyme attached to the helix. For mildly hydrophibic surfaces, such as a full maleimide-terminated SAM, these increased interactions are destabilizing. This was confirmed experimentally by the reduction in thermal stability for beta-galactosidase attached by the loop region. Using SFG, it was shown that the distribution of orientations of an enzyme attached to a loop is greater than to an enzyme tethered to a helix. By varying the electrostatic properties of the terminal groups used in the SAMs, it was shown that beta-galactosidase immobilized onto SAMs containing a mixture of positively and negatively terminated residues retained a higher level of specific activity than surfaces terminated with either uncharged hydrophilic or hydrophobic surfaces. Thermal stability was highest on uncharged hydrophilic surfaces. Overall, we were able to develop a molecular level model for the behavior of surface attached enzymes, and a potential approach for predicting approaches for engineering tethered enzyme systems.
Advisors/Committee Members: Marsh, E Neil G. (committee member), Chen, Zhan (committee member), Walter, Nils G. (committee member), O'Brien, Patrick (committee member).
Subjects/Keywords: Enzyme Immobilization; Surface Attachment; Biological Chemistry; Science
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Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Ogorzalek, T. L. (2015). Examining the Behavior of Surface Tethered Enzymes. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/111541
Chicago Manual of Style (16th Edition):
Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Doctoral Dissertation, University of Michigan. Accessed April 14, 2021.
http://hdl.handle.net/2027.42/111541.
MLA Handbook (7th Edition):
Ogorzalek, Tadeusz L. “Examining the Behavior of Surface Tethered Enzymes.” 2015. Web. 14 Apr 2021.
Vancouver:
Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/2027.42/111541.
Council of Science Editors:
Ogorzalek TL. Examining the Behavior of Surface Tethered Enzymes. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/111541

Cornell University
15.
Andler, Stephanie.
Immobilized Enzymes for Waste Stream Valorization.
Degree: PhD, Food Science and Technology, 2018, Cornell University
URL: http://hdl.handle.net/1813/59598
► Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes…
(more)
▼ Food industry waste streams represent a significant environmental burden. These waste streams, rich in sugars, proteins, and lipids, can be transformed into value-added products. Enzymes provide a selective and greener route of synthesis, but lack stability in extreme environments, and the ability to be reused. Therefore, the present work studied immobilized enzymes for practical application in two major waste streams: oil and dairy. First, lipase B from Candida antarctica (E.C. 3.1.1.3) was immobilized within cross-linked microparticles (CLMP) displaying varying scales of support, and the influence of these nano- and macroscale support materials on lipase activity and stability was studied. Kinetic analysis and extreme environment testing revealed that that polydicyclopentadiene macrostructure had the greatest influence on stability. These CLMPs successfully produced surfactants from lauric acid and D-glucose. However, the ~10 µm particles were unrecoverable from viscous solvents. Next, lipase was immobilized into macroscale, polymerized high internal phase emulsions (HIPE) to facilitate ease of handling. After fabrication, HIPEs displayed an increase in activity (139±9.7%) over native lipase. However, lipase was weakly immobilized, and retained less than 10% relative activity after 5 washes. HIPEs retained above 40% relative activity after exposure to pH 3, 7, and 10 for 24 hours, effectively expanding the working range of lipase. HIPEs also displayed an increase in relative activity after storage at 20 ˚C and 40 ˚C for 24 hours and retained greater than 40% activity after storage in 50 ˚C and 60 ˚C for 24 hours. These macroscale HIPEs were recoverable from reaction media but require further optimization to increase protein retention. Finally, β-galactosidase from Aspergillus oryzae (E.C. 3.2.1.23) and glucose isomerase (E.C. 5.3.1.5) were co-immobilized within a cross-linked enzyme aggregate (combi-CLEA) for dairy waste upcycling. The β-galactosidase within the combi-CLEA retained approximately 10% activity after
immobilization, but activity was unable to be detected from glucose isomerase. Additional optimization of the combi-CLEA is necessary before application testing in whey permeate. Applications-based approaches to enzyme
immobilization are necessary to create low-cost, industrially relevant catalysts. Through the understanding of support material influences, and extreme environment testing, enzymes have the potential to increase the overall sustainability of food processing.
Advisors/Committee Members: Goddard, Julie Melissa (chair), Sacks, Gavin Lavi (committee member), Nugen, Sam Rasmussen (committee member).
Subjects/Keywords: bioprocessing; enzyme; immobilization; waste stream; Food science
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APA (6th Edition):
Andler, S. (2018). Immobilized Enzymes for Waste Stream Valorization. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/59598
Chicago Manual of Style (16th Edition):
Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization.” 2018. Doctoral Dissertation, Cornell University. Accessed April 14, 2021.
http://hdl.handle.net/1813/59598.
MLA Handbook (7th Edition):
Andler, Stephanie. “Immobilized Enzymes for Waste Stream Valorization.” 2018. Web. 14 Apr 2021.
Vancouver:
Andler S. Immobilized Enzymes for Waste Stream Valorization. [Internet] [Doctoral dissertation]. Cornell University; 2018. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1813/59598.
Council of Science Editors:
Andler S. Immobilized Enzymes for Waste Stream Valorization. [Doctoral Dissertation]. Cornell University; 2018. Available from: http://hdl.handle.net/1813/59598

Texas A&M University
16.
Rathnayake, Sewwandi.
In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.
Degree: PhD, Soil Science, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173842
► The efficacy of in situ fixing of arsenic (As) and lead (Pb) in a highly contaminated soil was examined using chemical extractions for lability, bioavailability,…
(more)
▼ The efficacy of in situ fixing of arsenic (As) and lead (Pb) in a highly contaminated soil was examined using chemical extractions for lability, bioavailability, and bioaccessibility accompanied by spectroscopic verification. Soil Samples from a former smelting site in Stockton, Utah, contained 7,520 mg kg^-1 total As and 66,400 mg kg^-1 total Pb, more than 100 times background levels. The high total Pb and As concentrations coupled with high bioaccessibility (Physiologically Based Extraction Test: 246 mg kg^-1 As; 20,900 mg kg^-1 Pb) raise concerns of environmental and human health effects. Goethite, ferrihydrite, and high-iron water treatment residuals (Fe-WTR) were tested as amendments. The Fe-WTR applied at a 10:1 Fe:As mole ratio was the most effective, reducing water soluble As and Pb by 95% and 72%, respectively. However, when soluble P was added to the soil to immobilize Pb, As lability significantly increased due to the similar chemistries of phosphate and arsenate. When soluble P and Fe-WTR were added in sequence, 90% reduction of bioaccessible As and 60% reduction in plant available As (uptake by Pteris quadriaurita) were observed. Speculation that the efficacy of Fe-WTR as an in situ immobilizing agent was due to the presence of high surface area and highly reactive, poorly crystalline Fe oxides was supported by μ-XRF and μ-XANES analyses. Unamended, contaminated soil was observed to be a mixture of As(III) and As(V) oxides whereas amendment with Fe-WTR resulted in a shift to As(V) solid phases dominated by As(V) oxides and sulfates, all containing Fe(III). Identified minerals included ferric arsenate sulfate [Fev5(AsOv4)v3(SOv4)v3], yukonite [Cav7Fev11(AsOv4)v9Ov10·24.3Hv2O], and scorodite (FeAsOv4·2Hv2O). When soluble phosphate was added as a single amendment, As mineralogy included significant concentrations of As(V) oxide, Asv2Ov5. For Pb, addition of soluble P increased hydropyromophite [Pbv5(POv4)v3OH] content to 70%, but when Fe-WTR was added after soluble P, hydropyromophite never exceeded 40%. Just as the addition of soluble P interfered with in situ fixing of As, Fe-WTR partially prevented the conversion of Pb to pyromorphite minerals. The μ-XRF and μ-XANES analyses give physical evidence of the chemical shifts suggested in the bioaccessibility and chemical lability tests.
Advisors/Committee Members: Schwab, Paul (advisor), Morgan, Cristine (committee member), Deng, Youjun (committee member), Karthikeyan, R (committee member).
Subjects/Keywords: Arsenic; Lead; In-situ immobilization; Bioavailability; Bioaccessibility
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APA ·
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MLA ·
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Export
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APA (6th Edition):
Rathnayake, S. (2018). In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173842
Chicago Manual of Style (16th Edition):
Rathnayake, Sewwandi. “In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.” 2018. Doctoral Dissertation, Texas A&M University. Accessed April 14, 2021.
http://hdl.handle.net/1969.1/173842.
MLA Handbook (7th Edition):
Rathnayake, Sewwandi. “In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.” 2018. Web. 14 Apr 2021.
Vancouver:
Rathnayake S. In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1969.1/173842.
Council of Science Editors:
Rathnayake S. In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173842

Texas A&M University
17.
Rathnayake, Sewwandi.
In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.
Degree: PhD, Soil Science, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173747
► The efficacy of in situ fixing of arsenic (As) and lead (Pb) in a highly contaminated soil was examined using chemical extractions for lability, bioavailability,…
(more)
▼ The efficacy of in situ fixing of arsenic (As) and lead (Pb) in a highly contaminated soil was examined using chemical extractions for lability, bioavailability, and bioaccessibility accompanied by spectroscopic verification. Soil Samples from a former smelting site in Stockton, Utah, contained 7,520 mg kg^-1 total As and 66,400 mg kg^-1 total Pb, more than 100 times background levels. The high total Pb and As concentrations coupled with high bioaccessibility (Physiologically Based Extraction Test: 246 mg kg^-1 As; 20,900 mg kg^-1 Pb) raise concerns of environmental and human health effects. Goethite, ferrihydrite, and high-iron water treatment residuals (Fe-WTR) were tested as amendments. The Fe-WTR applied at a 10:1 Fe:As mole ratio was the most effective, reducing water soluble As and Pb by 95% and 72%, respectively. However, when soluble P was added to the soil to immobilize Pb, As lability significantly increased due to the similar chemistries of phosphate and arsenate. When soluble P and Fe-WTR were added in sequence, 90% reduction of bioaccessible As and 60% reduction in plant available As (uptake by Pteris quadriaurita) were observed. Speculation that the efficacy of Fe-WTR as an in situ immobilizing agent was due to the presence of high surface area and highly reactive, poorly crystalline Fe oxides was supported by μ-XRF and μ-XANES analyses. Unamended, contaminated soil was observed to be a mixture of As(III) and As(V) oxides whereas amendment with Fe-WTR resulted in a shift to As(V) solid phases dominated by As(V) oxides and sulfates, all containing Fe(III). Identified minerals included ferric arsenate sulfate [Fev5(AsOv4)v3(SOv4)v3], yukonite [Cav7Fev11(AsOv4)v9Ov10·24.3Hv2O], and scorodite (FeAsOv4·2Hv2O). When soluble phosphate was added as a single amendment, As mineralogy included significant concentrations of As(V) oxide, Asv2Ov5. For Pb, addition of soluble P increased hydropyromophite [Pbv5(POv4)v3OH] content to 70%, but when Fe-WTR was added after soluble P, hydropyromophite never exceeded 40%. Just as the addition of soluble P interfered with in situ fixing of As, Fe-WTR partially prevented the conversion of Pb to pyromorphite minerals. The μ-XRF and μ-XANES analyses give physical evidence of the chemical shifts suggested in the bioaccessibility and chemical lability tests.
Advisors/Committee Members: Schwab, Paul (advisor), Morgan, Cristine (committee member), Deng, Youjun (committee member), Karthikeyan, R (committee member).
Subjects/Keywords: Arsenic; Lead; In-situ immobilization; Bioavailability; Bioaccessibility
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rathnayake, S. (2018). In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173747
Chicago Manual of Style (16th Edition):
Rathnayake, Sewwandi. “In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.” 2018. Doctoral Dissertation, Texas A&M University. Accessed April 14, 2021.
http://hdl.handle.net/1969.1/173747.
MLA Handbook (7th Edition):
Rathnayake, Sewwandi. “In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil.” 2018. Web. 14 Apr 2021.
Vancouver:
Rathnayake S. In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1969.1/173747.
Council of Science Editors:
Rathnayake S. In Situ Immobilization of Arsenic and Lead in Smelter Contaminated Soil. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173747

University of Toronto
18.
Petryayeva, Eleonora.
Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.
Degree: 2012, University of Toronto
URL: http://hdl.handle.net/1807/32487
► The unique optical properties of quantum dots (QDs) have been widely used to develop bioassays based on Fluorescence Resonance Energy Transfer (FRET). The solid-phase assays…
(more)
▼ The unique optical properties of quantum dots (QDs) have been widely used to develop bioassays based on Fluorescence Resonance Energy Transfer (FRET). The solid-phase assays using QDs as FRET donors have numerous practical advantages, including at least 10-fold enhancement in FRET efficiency, which is not immediately explained by theoretical predictions that model energy transfer processes of QDs in two-dimensional layers. Donor-acceptor separation distance, acceptor and donor concentrations were found to influence FRET efficiency in solid-phase assays. A novel immobilization strategy was implemented which made use of the high affinity of imidazole moieties to QD shells to build solid-phase QD bioassays. A 96-well polystyrene plate is presented as a platform suitable for rapid and convenient multiplexed detection. A typical microtiter plate reader was shown to be capable of discriminating different FRET pairs to picomol detection levels of target oligonucleotides. Furthermore, the QD-FRET bioassays provided for mismatch discrimination, and multiple cycles of regeneration were also demonstrated.
MAST
Advisors/Committee Members: Krull, Ulrich Jorg, Chemistry.
Subjects/Keywords: quantum dots; fluorescence; hybridization; immobilization; 0486
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Petryayeva, E. (2012). Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/32487
Chicago Manual of Style (16th Edition):
Petryayeva, Eleonora. “Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.” 2012. Masters Thesis, University of Toronto. Accessed April 14, 2021.
http://hdl.handle.net/1807/32487.
MLA Handbook (7th Edition):
Petryayeva, Eleonora. “Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors.” 2012. Web. 14 Apr 2021.
Vancouver:
Petryayeva E. Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1807/32487.
Council of Science Editors:
Petryayeva E. Fluorescence Resonance Energy Transfer between a Monolayer of Quantum Dots as Donors adjacent to a Monolayer of Biorecognition Elements as Acceptors. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/32487

University of Adelaide
19.
Misson, Mailin.
Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.
Degree: 2016, University of Adelaide
URL: http://hdl.handle.net/2440/103557
► Enzyme immobilization has been recognized as a promising technique to enhance enzyme stability, activity and reusability for the development of cost-effective, green and sustainable biotechnological…
(more)
▼ Enzyme
immobilization has been recognized as a promising technique to enhance enzyme stability, activity and reusability for the development of cost-effective, green and sustainable biotechnological processes. Recent development in nanotechnology has opened a new frontier for diverse nano-scale enzyme carriers. The
immobilization of enzyme onto nanomaterials produces a nanobiocatalyst assembly, which maximizes reaction efficiencies by favoring desirable chemical reaction kinetics and selectivity for substrates, while the unique properties of nanocarriers offer a revolution of biocatalyst applications in the bioprocessing field. Nevertheless, the issues of enzyme leakage and conformational changes make the translation of the biocatalyst technology into commercial practices technically challenging and economically infeasible. Hence, investigating new technologies for fabricating the nanobiocatalyst with promising biocatalytic activities and functionalities is of great importance. In this PhD research, nanoparticle- and nanofiber-based enzyme carriers were developed and explored to immobilize β-galactosidase for conversion of lactose from dairy industry wastes into galacto-oligosaccharide (GOS) as a high value product. The structure-function relationship for the nanocarrier, the enzyme-nanocarrier microenvironment and the enzyme-nanocarrier nanobiocatalyst structure were extensively evaluated, aiming to enhance the bioengineering performance of the nanobiocatalysts. Dendrimer-like silica nanoparticles (HPSNs) with hierarchical pores were synthesized, characterized and functionalized with amino (NH2) and carboxyl group (COOH) to facilitate enzyme binding. Our findings revealed that surface functionalization can promote enzyme affinity towards the nanomaterial interface and selectively enhance enzyme reusability and its catalytic activity for improving the GOS production yield. A systematic synthesis of polystyrene nanofibers (PSNFs) was executed by optimizing key fabrication parameters using the electrospinning technique, including polymer concentration, electric voltage and distance between discharge needle tips and the collector. Surface modification of the PSNF was found to improve enzyme loading and activity. In addition, the local microenvironment of the nanobiocatalysts was able to optimize the enzyme selectivity and specificity, resulting in favouring transgalactosylation over hydrolysis for the lactose bioconversion. Further investigation to enhance the enzyme stability and catalytic activity at various operating conditions was conducted. PSNFs were chosen as the enzyme carrier owing to their scaling up potential in a manufacturing reactor system with their excellent mechanical and structural properties. Immobilizing β-galactosidase on the modified PSNF surface facilitated formation of stable enzyme binding and exhibited distinguished catalytic performance. Thermal and pH stability were improved significantly while the recyclability was enhanced from four to nine cycles. The evaluation of lactose conversion…
Advisors/Committee Members: Zhang, Hu (advisor), Jin, Bo (advisor), School of Chemical Engineering (school).
Subjects/Keywords: nandbiocatalysts; immobilization; β-galactosidase; nanofibers; nanoparticles; bioprocess
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Misson, M. (2016). Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103557
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Misson, Mailin. “Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.” 2016. Thesis, University of Adelaide. Accessed April 14, 2021.
http://hdl.handle.net/2440/103557.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Misson, Mailin. “Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production.” 2016. Web. 14 Apr 2021.
Vancouver:
Misson M. Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/2440/103557.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Misson M. Preparation and characterization of β-galactosidase nanobiocatalysts and its application for galacto-oligosaccharides production. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/103557
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto
20.
Odedra, Devangbhai.
Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.
Degree: 2011, University of Toronto
URL: http://hdl.handle.net/1807/29595
► A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We…
(more)
▼ A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We hypothesized here that immobilizing a gradient of vascular endothelial growth factor (VEGF-165) would guide endothelial cells into the interior of the scaffold thereby enhancing angiogenesis. The protein was immobilized onto a collagen scaffold through carbodiimide chemistry by one of the three methods experimented: placing 5 µl of the solution at the center of the scaffold to create a ~2 ng/ml/mm gradient in a radial direction. D4T endothelial cells were observed to be guided by this VEGF-165 gradient deep into the center of the scaffold compared to both uniformly immobilized VEGF-165 and VEGF-free controls. We concluded that the VEGF-165 gradient scaffolds promoted the migration, and not proliferation, of cells deep into the scaffold. These gradient scaffolds provide the foundation for future in vivo tissue engineering studies.
MAST
Advisors/Committee Members: Radisic, Milica, Shoichet, Molly, Chemical Engineering and Applied Chemistry.
Subjects/Keywords: gradient; VEGF; Tissue Engineering; collagen; immobilization; 0541
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Odedra, D. (2011). Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/29595
Chicago Manual of Style (16th Edition):
Odedra, Devangbhai. “Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.” 2011. Masters Thesis, University of Toronto. Accessed April 14, 2021.
http://hdl.handle.net/1807/29595.
MLA Handbook (7th Edition):
Odedra, Devangbhai. “Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.” 2011. Web. 14 Apr 2021.
Vancouver:
Odedra D. Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1807/29595.
Council of Science Editors:
Odedra D. Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29595

Univerzitet u Beogradu
21.
Radovanović, Mirjana N., 1984-.
Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином.
Degree: Tehnološko-metalurški fakultet, 2018, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get
► Технолошко инжењерство - Биохемијско инжењерство и биотехнологија / Engineering technology - Biochemical Engineering and Biotechnology
Циљ ове дисертације био је имобилизација α-амилазе на полианилину и…
(more)
▼ Технолошко инжењерство - Биохемијско инжењерство и
биотехнологија / Engineering technology - Biochemical Engineering
and Biotechnology
Циљ ове дисертације био је имобилизација α-амилазе
на полианилину и магнетним честицама модификованим полианилином
методом адсорпције ради добијања вишефункционалног
биокатализаторског система погодног за вишекратну хидролизу скроба.
Истраживања у оквиру ове тезе могу се поделити у три дела. У првом
делу рада извршена је синтеза и карактеризација честица носача и
карактеризација α-амилазe из Bacillus licheniformis ензимског
препарата Termamyl. Магнетне честице (MAG) синтетисане су
копреципитацијом FeCl3 и FeSO4 у базној средини, а затим обложене
полианилинском превлаком (MAG-PANI). Честице полианилина (PANI)
синтетисане су хемијски на граници фаза двофазног система
вода/хлороформ. Испитане су морфолошке особине, магнетна својства,
IR спектри, расподела величине честица и зета потенцијала MAG и
MAG-PANI. Показано је да су синтетисани агломерати наночестица MAG
и MAG-PANI. Површина магнетних честица униформно је пресвучена
полианилином влакнасте структуре. Честице MAG карактерише
бимодална, а MAG-PANI тримодална расподела величина честица. У
другом делу рада извршена је имобилизација ензима α-амилазе на
честицaма MAG, MAG-PANI и PANI. Одређени су оптимални услови
имобилизације на MAG-PANI: време, почетна концентрација ензима, рН
и температура. Испитивањем кинетичких параметара Km и Vm утврђено
је да имобилисана α-амилаза на МАG-PANI показује већи афинитет
према супстрату него имобилисана α-амилаза на MAG, а нижи афинитет
од слободне α-амилазе. Утврђен је Ленгмиров (Langmuir) модел
адсорпције α-амилазе на честицама MAG-PANI. У трећем делу рада
испитана је стабилност имобилисане α-амилазе, могућност употребе
кроз више циклуса као и оперативна стабилност у проточном
биореактору са континуалним и повратним током. Утврђена је рН
стабилизација α-амилазе имобилизацијом на MAG честицама и померање
температурног оптимума имобилизацијом на MAG-PANI. Имобилисана
α-амилаза на MAG-PANI задржала је око 55,5% почетне активности
након 9 циклуса коришћења и око 80,3% почетне активности након 50
дана чувања. Време полу-живота биокатализатора при континуалним
условима рада било је 6,2 h.
Advisors/Committee Members: Knežević-Jugović, Zorica, 1969-.
Subjects/Keywords: α-amylase; immobilization; magnetic particles;
polyaniline
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Radovanović, Mirjana N., 1. (2018). Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Radovanović, Mirjana N., 1984-. “Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином.” 2018. Thesis, Univerzitet u Beogradu. Accessed April 14, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Radovanović, Mirjana N., 1984-. “Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином.” 2018. Web. 14 Apr 2021.
Vancouver:
Radovanović, Mirjana N. 1. Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Apr 14].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Radovanović, Mirjana N. 1. Имобилизација алфа-амилазе на полианилину и магнетним
честицама модификованим полианилином. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18780/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Uppsala University
22.
Schönn, Mikael Andersson.
Promoter regulation : designing cells for biotechnological applications.
Degree: Molecular Biomimetics, 2016, Uppsala University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502
► The filamentous cyanobacteria Nostoc punctiforme ATCC 29133 is a model species fordevelopment of sustainable production methods of numerous compounds. One of its uniquefeatures is…
(more)
▼ The filamentous cyanobacteria Nostoc punctiforme ATCC 29133 is a model species fordevelopment of sustainable production methods of numerous compounds. One of its uniquefeatures is the anaerobic environment of the strains nitrogen fixing heterocyst cells. To be ableto properly utilize this environment, more knowledge regarding what regulates cell specificexpression is required. In this study, three motifs of the NsiR I promoter of Anabaena sp.PCC 7120 was studied in this system utilizing YFP-fluorescence as a reporter to determinetheir impact on spatial expression pattern. Investigations were performed on immobilizedcells with the use of confocal microscopy and results point towards sigma factor regulation.
Subjects/Keywords: Cyanobacteria; heterocyst; promoter; specificity; confocal microscopy; immobilization
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Schönn, M. A. (2016). Promoter regulation : designing cells for biotechnological applications. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Schönn, Mikael Andersson. “Promoter regulation : designing cells for biotechnological applications.” 2016. Thesis, Uppsala University. Accessed April 14, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Schönn, Mikael Andersson. “Promoter regulation : designing cells for biotechnological applications.” 2016. Web. 14 Apr 2021.
Vancouver:
Schönn MA. Promoter regulation : designing cells for biotechnological applications. [Internet] [Thesis]. Uppsala University; 2016. [cited 2021 Apr 14].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Schönn MA. Promoter regulation : designing cells for biotechnological applications. [Thesis]. Uppsala University; 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297502
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Politècnica de València
23.
Tortajada Serra, Marta.
Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
.
Degree: 2012, Universitat Politècnica de València
URL: http://hdl.handle.net/10251/16800
► El objetivo general de la presente tesis doctoral es el desarrollo de herramientas para la obtencion, produccion y aplicacion de dos enzimas glicosidicas: �¿-L-arabinofuranosidasa proveniente…
(more)
▼ El objetivo general de la presente tesis doctoral es el desarrollo de herramientas para la
obtencion, produccion y aplicacion de dos enzimas glicosidicas: �¿-L-arabinofuranosidasa
proveniente del hongo Aspergillus niger (Abf) y �À-D-glucosidasa (Bgl), proveniente de la
levadura Candida molischiana. Estas hidrolasas se emplean en la liberacion de azucares en
procesos de conversion de biomasa y en la industria alimentaria, pero tambien en la sintesis de
aminoglicosidos, glicoconjugados y oligosacaridos, compuestos de alto valor anadido para la
industria quimico-farmaceutica. Las enzimas se han expresado en la levadura metilotrofica
Pichia pastoris, y se han purificado para caracterizar sus propiedades bioquimicas. Asimismo,
se ha comprobado su capacidad para catalizar reacciones de transglicosilacion con alto
rendimiento. En relacion a su produccion, se ha establecido y validado un modelo basado en
restricciones del metabolismo de Pichia pastoris, evaluando su consistencia mediante analisis
de flujos metabolicos posibilistico. El modelo permite estimar la tasa de crecimiento y la
distribucion de flujos intracelulares a partir de unos pocos flujos extracelulares medidos
experimentalmente. Adicionalmente, el modelo se ha extendido para estimar la productividad
de proteina recombinante, y se ha empleado para analizar diferentes condiciones de cultivo de
las cepas transgenicas que sobreproducen las enzimas Abf y Bgl. Finalmente, las enzimas se
han inmobilizado en organosilicas bimodales de la familia UVM-7. Los biocatalizadores
resultantes se han caracterizado bioquimica y fisico-quimicamente y se han evaluado en
diferentes aplicaciones de interes biotecnologico.
Advisors/Committee Members: Picó Marco, Jesús Andrés (advisor), Ramon Vidal, Daniel (advisor).
Subjects/Keywords: Glycosidases;
Pichia pastoris;
Constraint-based model;
Immobilization
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APA (6th Edition):
Tortajada Serra, M. (2012). Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
. (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/16800
Chicago Manual of Style (16th Edition):
Tortajada Serra, Marta. “Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
.” 2012. Doctoral Dissertation, Universitat Politècnica de València. Accessed April 14, 2021.
http://hdl.handle.net/10251/16800.
MLA Handbook (7th Edition):
Tortajada Serra, Marta. “Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
.” 2012. Web. 14 Apr 2021.
Vancouver:
Tortajada Serra M. Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
. [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2012. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10251/16800.
Council of Science Editors:
Tortajada Serra M. Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
. [Doctoral Dissertation]. Universitat Politècnica de València; 2012. Available from: http://hdl.handle.net/10251/16800

Arizona State University
24.
Bone, Stephanie Rosalia.
Immobilization of T4 on Modified Silica Particles.
Degree: Civil and Environmental Engineering, 2017, Arizona State University
URL: http://repository.asu.edu/items/44290
► Bacteriophage provide high specificity to bacteria; receiving interest in various applications and have been used as target recognition tools in designing bioactive surfaces. Several current…
(more)
▼ Bacteriophage provide high specificity to bacteria;
receiving interest in various applications and have been used as
target recognition tools in designing bioactive surfaces. Several
current immobilization strategies to detect and capture
bacteriophage require non-deliverable bioactive substrates or
modifying the chemistry of the phage, procedures that are labor
intensive and can damage the integrity of the virus. The aim of
this research was to develop the framework to physisorb and
chemisorb T4 coliphage on varied sized functionalized silica
particles while retaining its infectivity. First, silica surface
modification, silanization, altered pristine silica colloids to
positively, amine coated silica. The phages remain infective to
their host bacteria while adsorbed on the surface of the silica
particles. It is reported that the number of infective phage bound
to the silica is enhanced by the immobilization method. It was
determined that covalent attachment yielded 106 PFU/ml while
electrostatic attachment resulted in 105 PFU/ml.
Subjects/Keywords: Environmental engineering; bacteriophage; bacteriophage immobilization; silanization
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MLA ·
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APA (6th Edition):
Bone, S. R. (2017). Immobilization of T4 on Modified Silica Particles. (Masters Thesis). Arizona State University. Retrieved from http://repository.asu.edu/items/44290
Chicago Manual of Style (16th Edition):
Bone, Stephanie Rosalia. “Immobilization of T4 on Modified Silica Particles.” 2017. Masters Thesis, Arizona State University. Accessed April 14, 2021.
http://repository.asu.edu/items/44290.
MLA Handbook (7th Edition):
Bone, Stephanie Rosalia. “Immobilization of T4 on Modified Silica Particles.” 2017. Web. 14 Apr 2021.
Vancouver:
Bone SR. Immobilization of T4 on Modified Silica Particles. [Internet] [Masters thesis]. Arizona State University; 2017. [cited 2021 Apr 14].
Available from: http://repository.asu.edu/items/44290.
Council of Science Editors:
Bone SR. Immobilization of T4 on Modified Silica Particles. [Masters Thesis]. Arizona State University; 2017. Available from: http://repository.asu.edu/items/44290

University of New South Wales
25.
Tregubov, Andrey Aleksandrovich.
Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.
Degree: Chemistry, 2013, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/53291
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true
► This thesis describes the study of the immobilization of Rh(I) complexes with N,N (bis(pyrazol-1-yl)methane (bpm), bis(N-methylimidazol-2-yl)methane (bim), and 4-(pyrazol-1-yl)methyl)-1-H-1,2,3-triazole (PyT)) and N,P (1-[2-(diphenylphosphino)ethyl]pyrazole (dmPyP) ligands…
(more)
▼ This thesis describes the study of the
immobilization of Rh(I) complexes with N,N (bis(pyrazol-1-yl)methane (bpm), bis(N-methylimidazol-2-yl)methane (bim), and 4-(pyrazol-1-yl)methyl)-1-H-1,2,3-triazole (PyT)) and N,P (1-[2-(diphenylphosphino)ethyl]pyrazole (dmPyP) ligands onto carbon surfaces (glassy carbon electrode (GC), glassy carbon beads (GCB), carbon black (XC)) via robust carbon-carbon bonds. The immobilized Rh(I) complexes were employed as recoverable catalysts for hydromamination and hydroalkoxylation reactions.A series of aforementioned N,N ligands and one N,P ligand precursor were functionalized with aniline and aniline hydrochloride respectively. The treatment of the aniline-functionalized ligands and aniline hydrochloride-functionalized ligand precursor with HNO2 resulted in the transformation of the NH2 functionality into diazonium one. The electrochemical reduction of the diazonium functionality of the N,N ligands and N,P ligand precursor resulted in the surface attachment of these compounds onto glassy carbon electrode surfaces. The treatment of the surface-bound N,P ligand precursor with LiPPh2 resulted in the formation of the surface-bound N,P ligand (dmPyP). The complexation of [Rh(CO)2(μ-Cl)]2 with the immobilized N,N and N,P ligands led to the formation of immobilized Rh(I) complexes of these ligands. XPS confirmed the surface attachment of the N,N ligands and N,P ligand precursor as well as the success of the synthesis of the N,P ligand on the surface and complexation between immobilized ligands and the Rh precursor. The surface coverage of immobilized Rh(I) complexes was evaluated using CV and was found to be close to being a monolayer. The immobilized Rh(I) complexes were active as catalysts for the intramolecular hydroamination and dihydroalkoxylation reactions with extremely high TONs in comparison with their homogeneous counterparts. Studies of the recovered catalyst samples showed that Rh leached from the surface while the ligand remained intact. The aniline-functionalized N,N ligands were also attached onto glassy carbon electrodes without electrochemical assistance by dipping the electrode into a solution of aryl diazonium salt. Complexation of the ligands immobilized without electrochemical assistance and [Rh(CO)2(μ-Cl)]2 resulted in immobilized Rh(I) complexes with these ligands. XPS confirmed that the immobilized species had the same nature as those immobilized using electrochemical reduction and they were effective catalysts for dihydroalkoxylation. The surface coverage of these complexes was evaluated by means of CV and was found to be close to being a monolayer. The ligand bpm was also immobilized onto carbon powders (GCB and XC) without electrochemical assistance. The immobilized support-[Rh(bpm)(CO)2] complexes were obtained by the treatment of bpm-functionalized supports with [Rh(CO)2(μ-Cl)]2. XPS confirmed the ligand attachment onto the surface of carbon powders and complexation with Rh. TGA also confirmed the covalent
immobilization of the ligand and was used to determine the…
Advisors/Committee Members: Messerle, Barbara, Chemistry, Faculty of Science, UNSW.
Subjects/Keywords: Solid-state NMR; Catalyst immobilization; Electrochemistry; Carbon
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Tregubov, A. A. (2013). Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Tregubov, Andrey Aleksandrovich. “Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.” 2013. Doctoral Dissertation, University of New South Wales. Accessed April 14, 2021.
http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true.
MLA Handbook (7th Edition):
Tregubov, Andrey Aleksandrovich. “Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts.” 2013. Web. 14 Apr 2021.
Vancouver:
Tregubov AA. Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Apr 14].
Available from: http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true.
Council of Science Editors:
Tregubov AA. Rh(I) complexes with N,N and N,P ligands immobilized on carbon surfaces – recyclable catalysts. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/53291 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11986/SOURCE02?view=true

University of New South Wales
26.
Hou, Jingwei.
Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.
Degree: Chemical Sciences & Engineering, 2014, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/54572
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true
► Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant…
(more)
▼ Over the last decades, the sharp increases in industrial polycarbonate and epoxy production and fossil fuel consumption have resulted in emerging environmental challenges like micropollutant and greenhouse gas, which require novel mitigation approaches. Enzyme catalyzed reactions have been recognized as promising approaches for many environmental applications. Among different enzymes, laccase and carbonic anhydrase have attracted wide spread interest in wastewater treatment and CO2 capture respectively. However, the application of free enzyme is restricted due to the difficulty in recycle and reuse. Therefore, efficient enzyme
immobilization and bio-reactor design are required.Based on a series of comprehensive experimental researches, the performance of TiO2 nanoparticle and TiO2 nanoparticle functionalized membrane as laccase
immobilization support has been evaluated. Different
immobilization techniques were compared and the sequential process was regarded as the optimal option due to its relatively high activity and stability. It was also found that the nanoparticle size, surface charge, and laccase accessibility also had significant effect on the immobilized laccase performance.TiO2 functionalized membranes were prepared with both nanoparticle blending and sol-gel coating processes, then laccase was immobilized on the resultant membranes. Compared with the blended membrane, the coated membrane provided more accessible TiO2 for laccase
immobilization thus resulted in better performances. The effect of coating layer surface architectural properties and the membrane pore size on the laccase
immobilization performance was also discussed.In this study, two laccase based membrane bioreactors were proposed for bisphenol-A (BPA) degradation: the bio-catalytic membrane reactor and the bio-catalytic nanoparticle suspension membrane hybrid reactor. For both reactors, satisfactory BPA removal was achieved through enzymatically degradation and membrane filtration process. In addition, the performance of the hybrid reactor under simulated wastewater harsh conditions was assessed to evaluate the feasibility of using such reactors for municipal water treatment.Finally, the feasibility of applying the TiO2 nanoparticle and TiO2 coated polymer membrane as carbonic anhydrase
immobilization support was also explored. This study also prepared a novel TiO2 functionalized hydrophilic-hydrophobic membrane for carbonic anhydrase
immobilization. Such a bio-catalytic membrane was further used for CO2 hydration with a gas-liquid contactor, and nearly 100 % improvement in CO2 hydration rate was obtained
Advisors/Committee Members: CHEN, VICKI, Chemical Sciences & Engineering, Faculty of Engineering, UNSW.
Subjects/Keywords: CO2 hydration; Enzyme immobilization; Micropollutant removal
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Hou, J. (2014). Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Doctoral Dissertation, University of New South Wales. Accessed April 14, 2021.
http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.
MLA Handbook (7th Edition):
Hou, Jingwei. “Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture.” 2014. Web. 14 Apr 2021.
Vancouver:
Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Internet] [Doctoral dissertation]. University of New South Wales; 2014. [cited 2021 Apr 14].
Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true.
Council of Science Editors:
Hou J. Titania Based Bio-catalytic Membrane Systems for Bisphenol-A Degradation and CO2 Capture. [Doctoral Dissertation]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/54572 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:35312/SOURCE02?view=true

University of Akron
27.
Chen, Xi.
Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers.
Degree: MS, Polymer Science, 2015, University of Akron
URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122
► Biomimetic catalysis has great impact on the development of the research of organic catalysis1. Immobilized enzyme used as catalysts has attracted a lot of attention…
(more)
▼ Biomimetic catalysis has great impact on the
development of the research of organic catalysis1. Immobilized
enzyme used as catalysts has attracted a lot of attention because
of its high stability and the excellent ability of recovering from
the reaction environment. Furthermore, the activity of immobilized
enzyme can be retained after long-term storage or exposure to high
temperatures2,3. In this study,
Immobilization of GOx was achieved
by adsorption and encapsulation of GOx onto porous polymer support,
and catalytic activity of GOx immobilized porous support was
evaluated by testing the formation of H2O2. On the other hand,
biological sequestration of carbon dioxide (CO2) is one of the
proposed approaches for CO2 unitization. Carbonic anhydrase was
chose as catalyst to study the catalytic reaction of CO2. The
reaction was in situ monitored by Attenuated total reflectance
(ATR), the preliminary results indicated the formation of
bicarbonate (HCO3-), which was evidenced by the IR characteristic
band at 1400 cm-1 4.
Advisors/Committee Members: Chuang, Steven S.C. (Advisor).
Subjects/Keywords: Polymers; Enzyme, immobilization, UV-vis, infrared
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, X. (2015). Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122
Chicago Manual of Style (16th Edition):
Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers.” 2015. Masters Thesis, University of Akron. Accessed April 14, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.
MLA Handbook (7th Edition):
Chen, Xi. “Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers.” 2015. Web. 14 Apr 2021.
Vancouver:
Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers. [Internet] [Masters thesis]. University of Akron; 2015. [cited 2021 Apr 14].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122.
Council of Science Editors:
Chen X. Infrared and Uv-Vis Spectroscopic Studies of Catalytic
Reaction of Enzymes and Immobilization Enzyme on Porous
Polymers. [Masters Thesis]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428327122

Texas A&M University
28.
Cluff, Kyle James.
Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials.
Degree: PhD, Chemistry, 2016, Texas A&M University
URL: http://hdl.handle.net/1969.1/192012
► This thesis will explore two different facets of the quest for superior immobilized catalysts: (1) cyclotrimerization of acetylenes with nickel catalysts and (2) solid-state NMR…
(more)
▼ This thesis will explore two different facets of the quest for superior immobilized catalysts: (1) cyclotrimerization of acetylenes with nickel catalysts and (2) solid-state NMR studies of the adsorption and subsequent mobility of organometallic solids on silica gel and other relevant surfaces.
The cyclotrimerization of acetylenes has a wide range of applications and can be catalyzed by inexpensive immobilized nickel complexes. However, the selectivity of these catalysts changes over time, and the activity declines. In this study, tridentate ligands are employed to reduce leaching and decomposition.
The chelate nickel complexes incorporating tridentate phosphine ligands (CO)Ni[(PPh2CH2)3CMe] (2), (CO)Ni[(PPh2CH2CH2)3SiMe] (6), and Ph3PNi[(PPh2CH2CH2)3SiMe] (7), as well as the bidentate complex (CO)2Ni[(PPh2CH2)2CMeCH2PPh2] (3) and the heterobimetallic complex (CO)2Ni[(PPh2CH2)2CMeCH2Ph2PAuCl] (4) have been synthesized and characterized in solution and in the solid state. While complexes 2-4 displayed low catalytic activity, complex 7 led to 100% conversion of the substrate within four hours and was highly selective throughout the catalytic reaction.
Various metallocenes have been adsorbed on high surface area materials including mesoporous silica, carbon nanotubes and activated carbon. The adsorption progresses quickly onto a favorable support, even when the solids contact each other without a solvent, and is practically complete within two hours. Its progress is monitored by 1H, 13C, and 2H solid-state NMR spectroscopy. The transition from the crystal lattice to the surface species that are highly mobile is proven by strongly reduced chemical shift anisotropies and diminished dipolar interactions. All solid-state NMR spectra indicate that the transition from a monolayer to the crystalline state is abrupt, and no multiple layers form on the surfaces. A correlation between surface coverage and 2H residual linewidths has been established. Besides a hydrophobic dry silica surface, wet and TMScapped silica have been used as supports. The 2H MAS spectra of surface-adsorbed ferrocene-d2 prove that the motion of the metallocenes on the surfaces is fast and nearly isotropic, as in solution. Consequently, it is demonstrated that 1H and 13C NMR spectra of adsorbed ferrocene can be recorded using a conventional liquids NMR instrument. Additionally, it is possible to visually monitor the adsorption and translational mobility of ferrocene and other metallocenes in large pieces of silica gel.
Advisors/Committee Members: Bluemel, Janet (advisor), Darensbourg, Marcetta (committee member), Schweikert, Emile (committee member), Ross, Joseph (committee member).
Subjects/Keywords: adsorption; surface; immobilization; catalysis; nickel; metallocene
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cluff, K. J. (2016). Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/192012
Chicago Manual of Style (16th Edition):
Cluff, Kyle James. “Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials.” 2016. Doctoral Dissertation, Texas A&M University. Accessed April 14, 2021.
http://hdl.handle.net/1969.1/192012.
MLA Handbook (7th Edition):
Cluff, Kyle James. “Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials.” 2016. Web. 14 Apr 2021.
Vancouver:
Cluff KJ. Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials. [Internet] [Doctoral dissertation]. Texas A&M University; 2016. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1969.1/192012.
Council of Science Editors:
Cluff KJ. Homogeneous and Immobilized Nickel Catalysts and Mobility Studies of Metallocenes Adsorbed on High Surface Area Materials. [Doctoral Dissertation]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/192012

King Abdullah University of Science and Technology
29.
Lloyd-Randol, Jennifer D.
Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.
Degree: Physical Science and Engineering (PSE) Division, 2012, King Abdullah University of Science and Technology
URL: http://hdl.handle.net/10754/224713
► The limited availability of fossil resourses mandates the development of new energy vectors, which is one of the Grand Challenges of the 21st Century [1].…
(more)
▼ The limited availability of fossil resourses mandates the development of new energy
vectors, which is one of the Grand Challenges of the 21st Century [1]. Biocatalytic
energy conversion is a promising solution to meet the increased energy demand of
industrialized societies. Applications of biocatalysis in the gas-phase are so far limited
to production of fine chemicals and pharmaceuticals. However, this technology
has the potential for large scale biocatalytic applications [2], e.g. for the formation of
novel energy carriers. The so-called solid/gas biocatalysis is defined as the application
of a biocatalyst immobilized on solid-phase support acting on gaseous substrates [3].
This process combines the advantages of bio-catalysis (green chemistry, mild reaction
conditions, high specicity & selectivity) and heterogeneous dynamic gas-phase
processes (low diffusion limitation, high conversion, simple scale-up).
This work presents the modifications of a PID Microactivity Reference reactor in
order to make it suitable for solid/gas biocatalysis. The reactor design requirements
are based on previously published laboratory scale solid/gas systems with a feed
of saturated vapors [4]. These vapors are produced in saturation
flasks, which were
designed and optimized during this project. Other modifications included relocation
of the gas mixing chamber, redesigning the location and heating mechanism for the
reactor tube, and heating of the outlet gas line.
The modified reactor system was verified based on the Candida antarctica lipase
B catalyzed transesterication of ethyl acetate with 1-hexanol to hexyl acetate and
ethanol and results were compared to liquid-phase model reactions. Products were
analyzed on line by a gas chromatograph with a
flame ionization detector. C. antarc-
tica physisorbed on silica particles produced a 50% conversion of hexanol at 40 C in
the gas-phase. A commercial immobilized lipase from Iris Biotech produced 99% and
97% conversions of hexanol in similar experiments.
This project achieved its goal to design, establish and successfully verify a solid/-
gas biocatalysis reactor. Future work will target optimization of the reactor's operating
conditions and the development of whole cell catalysts for energy production
reactions. Potential experiments include the study of hydrogenolytic carbon dioxide
reduction to methanol by free enzymes or methanogenic organisms [5], and the
investigation of hydrogen production by water splitting of algae or cyanobacteria.
Advisors/Committee Members: Eppinger, Jörg (advisor), Pinnau, Ingo (committee member), Takanabe, Kazuhiro (committee member).
Subjects/Keywords: Biocatalytic; Validation; Solid gas; Bioreactor; Immobilization
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lloyd-Randol, J. D. (2012). Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. (Thesis). King Abdullah University of Science and Technology. Retrieved from http://hdl.handle.net/10754/224713
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lloyd-Randol, Jennifer D. “Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.” 2012. Thesis, King Abdullah University of Science and Technology. Accessed April 14, 2021.
http://hdl.handle.net/10754/224713.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lloyd-Randol, Jennifer D. “Set-Up and Validation of a Dynamic Solid/Gas Bioreactor.” 2012. Web. 14 Apr 2021.
Vancouver:
Lloyd-Randol JD. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. [Internet] [Thesis]. King Abdullah University of Science and Technology; 2012. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10754/224713.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lloyd-Randol JD. Set-Up and Validation of a Dynamic Solid/Gas Bioreactor. [Thesis]. King Abdullah University of Science and Technology; 2012. Available from: http://hdl.handle.net/10754/224713
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Rios, Nathalia Saraiva.
Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens.
Degree: 2019, Brazil
URL: http://www.repositorio.ufc.br/handle/riufc/48256
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Rejected by Marlene Sousa ([email protected]), reason: Prezada Nathalia,…
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Rejected by Marlene Sousa ([email protected]), reason: Prezada Nathalia, existe a RESOLUÇÃO No 17/CEPE, 02 DE OUTUBRO DE 2017, que estabelece a normalização das dissertações e teses da UFC, em suas paginas pré-textuais e lista de referencias, pelas regras da ABNT. Por esse motivo, sugerimos adequar seu trabalho ao modelo do template, disponível em: http://www.biblioteca.ufc.br/educacao-de-usuarios/templates/ Vamos agora as correções, que vc deve realizar, sempre de acordo com o template: 1. Na Ficha catalográfica; Alterar no título o uso de caixa alta (somente a primeira letra do título, as siglas e os substantivos próprios ficam em maiúsculo as demais letras ficam minúsculas). Conforme está na aba: instruções de preenchimento. http://fichacatalografica.ufc.br/ 2. Na folha de
aprovação da banca e nas folhas que sucedem a esta, todas as informações devem ser em língua inglesa (menos o RESUMO). 3. Na folha de AGRADECIMENTOS, a Portaria nº 206, de 4 de setembro de 2018 da Capes informa que é obrigatório inserir um agradecimento à instituição referente ao apoio recebido pelo bolsista. A redação do agradecimento está expressa na portaria: This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. 4. O RESUMO deve vir antes do ABSTRACT. No Resumo e Abstract não colocar parágrafo ao iniciar o texto. 5. Nas listas de FIGURAS e TABELAS observe o alinhamento da margem dos títulos das figuras e tabelas, de modo que ao aumentar o número de dígitos das figuras elas fiquem no mesmo alinhamento de quando tinham menos dígito. Quando o título da figura ou da tabela não couber na mesma linha, sua continuação deve ficar na mesma margem da primeira letra da linha de cima e não voltar para a margem
do F de Figura ou do T de Tabela. Retire a divisão por capítulos que é usada em livros. 6. Para dissertações e teses use o termo SUMMARY e não CONTENTS que deve ser usado em livros. No SUMÁRIO não se coloca a divisão por capítulos. No SUMÁRIO vc deve observar o alinhamento, para quando crescerem os dígitos das seções fique o mesmo alinhamento de quando tinha apenas um dígito. Inclusive quando o titulo não couber na mesma linha, sua continuação deve ficar na mesma margem da primeira letra da palavra da linha de cima. No SUMÁRIO retirar a pontuação no final do ultimo dígito da numeração das seções. Ex. 1 INTRODUÇÃO ; 1.2 Objectives Atenciosamente, Marlene Rocha 3366-9620 /[email protected] on 2019-12-05T19:12:39Z (GMT)
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Advisors/Committee Members: Fernández-Lafuente, Roberto, Gonçalves, Luciana Rocha Barros.
Subjects/Keywords: Engenharia química; Lipase; Biocatalisadores; Immobilization; Pseudomonas fluorescens
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APA (6th Edition):
Rios, N. S. (2019). Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens. (Doctoral Dissertation). Brazil. Retrieved from http://www.repositorio.ufc.br/handle/riufc/48256
Chicago Manual of Style (16th Edition):
Rios, Nathalia Saraiva. “Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens.” 2019. Doctoral Dissertation, Brazil. Accessed April 14, 2021.
http://www.repositorio.ufc.br/handle/riufc/48256.
MLA Handbook (7th Edition):
Rios, Nathalia Saraiva. “Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens.” 2019. Web. 14 Apr 2021.
Vancouver:
Rios NS. Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens. [Internet] [Doctoral dissertation]. Brazil; 2019. [cited 2021 Apr 14].
Available from: http://www.repositorio.ufc.br/handle/riufc/48256.
Council of Science Editors:
Rios NS. Development of strategies for the production of biocatalysts through immobilization / co-immobilization of lipase from Pseudomonas fluorescens. [Doctoral Dissertation]. Brazil; 2019. Available from: http://www.repositorio.ufc.br/handle/riufc/48256
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