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You searched for subject:(immobilization of biocatalyst). Showing records 1 – 3 of 3 total matches.

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1. Reni, George. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.

Degree: Applied Chemistry, 2013, Cochin University of Science and Technology

Mesoporous silica nanoparticles provide a non-invasive and biocompatible delivery platform for a broad range of applications in therapeutics, pharmaceuticals and diagnosis. Additionally, mesoporous silica materials can be synthesized together with other nanomaterials to create new nanocomposites, opening up a wide variety of potential applications. The ready functionalization of silica materials makes them ideal candidates for bioapplications and catalysis. These properties of mesoporous silica like high surface areas, large pore volumes and ordered pore networks allow them for higher loading of drugs or biomolecules. Comparative studies have been made to evaluate the different procedures; much of the research to date has involved quick exploration of new methods and supports. Requirements for different enzymes may vary, and specific conditions may be needed for a particular application of an immobilized enzyme such as a highly rigid support. In this endeavor, mesoporous silica materials having different pore size were synthesized and easily modified with active functional groups and were evaluated for the immobilization of enzymes. In this work, Aspergillus niger glucoamylase, Bovine liver catalase, Candida rugosa lipase were immobilized onto support by adsorption and covalent binding. The structural properties of pure and immobilized supports are analyzed by various characterization techniques and are used for different reactions of industrial applications.

Cochin University of Science and Technology

Subjects/Keywords: Enzymes as biocatalyst; Immobilization; ideal enzyme supports; Enzyme immobilization; activity of enzymes; Enzyme adsorption isotherms

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APA (6th Edition):

Reni, G. (2013). Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Thesis, Cochin University of Science and Technology. Accessed May 30, 2020. http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Reni, George. “Catalysis by Enzymes immobilized on Tuned Mesoporous Silica.” 2013. Web. 30 May 2020.

Vancouver:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Internet] [Thesis]. Cochin University of Science and Technology; 2013. [cited 2020 May 30]. Available from: http://dyuthi.cusat.ac.in/purl/4627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Reni G. Catalysis by Enzymes immobilized on Tuned Mesoporous Silica. [Thesis]. Cochin University of Science and Technology; 2013. Available from: http://dyuthi.cusat.ac.in/purl/4627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. WANG WEN. Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis.

Degree: 2011, National University of Singapore

Subjects/Keywords: Functionalized Magnetic Nanoparticles; Efficient Biocatalysis; Recyclable Nano-biocatalyst; Immobilization of Enzymes; Core-shell; Stable and Active

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

WEN, W. (2011). Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/32983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

WEN, WANG. “Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis.” 2011. Thesis, National University of Singapore. Accessed May 30, 2020. http://scholarbank.nus.edu.sg/handle/10635/32983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

WEN, WANG. “Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis.” 2011. Web. 30 May 2020.

Vancouver:

WEN W. Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis. [Internet] [Thesis]. National University of Singapore; 2011. [cited 2020 May 30]. Available from: http://scholarbank.nus.edu.sg/handle/10635/32983.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

WEN W. Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis. [Thesis]. National University of Singapore; 2011. Available from: http://scholarbank.nus.edu.sg/handle/10635/32983

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Šulek, Franja. Nanostrukturirani materiali za imobilizacijo biokatalizatorja.

Degree: 2011, Univerza v Mariboru

V doktorski nalogi je nazorno predstavljen postopek sinteze magnetnih nanodelcev iz železovega oksida maghemita, γ-Fe2O3, za vezavo biokatalizatorja. Magnetni nanodelci, ki v zadnjih letih vse bolj pridobivajo na vrednosti kot potencialni encimski nosilci, so bili najprej sintetizirani z metodo obarjanja ali koprecipitacije železovih (II, Fe2+) in železovih (III, Fe3+) ionov v alkalnem mediju pri hitrem mešanju in visoki temperaturi. Površinska funkcionalizacija magnetnih nanodelcev je bila izvedena v dveh stopnjah. Primarna funkcionalna prevleka iz silicijevega dioksida (SiO2), ki delcem daje predvsem višjo stabilnost, je bila sintetizirana pri striktno kontroliranih reakcijskih pogojih iz natrijevega silikata ali Na2SiO3. Sekundarni funkcionalni sloj za doseganje višje funkcionalnosti in reaktivnosti površine nanodelcev je bil sintetiziran iz organskih molekul aminosilana ali 3-(2-aminoetilamino)-propil-dimetoksimetilsilana v kislem mediju. V nadaljnjem so bili tako površinsko spremenjeni magnetni nanodelci uporabljeni za imobilizacijo specifičnega biokatalizatorja holesterol oksidaze (ChOx, EC 1.1.3.6) iz Corynebacterium sp. Analiza magnetnih nanodelcev z imobilizirano ChOx je pokazala, da so imeli nanodelci značilno obliko kroglice ali sfere s povprečnim premerom 50,2 nm. Vezava holesterol oksidaze je bila uspešno potrjena tudi s FT-IR tehniko. Učinkovitost imobilizacije na magnetni nosilec je znašala 92 % pri uporabljeni koncentraciji encima 100 µg mL-1. Aktivnost imobilizirane ChOx na magnetne nanodelce, prevlečene s tanko plastjo silikatne prevleke premera 3 nm, je bila 57 % v primerjavi z aktivnostjo ChOx. Študija vpliva pH-vrednosti in temperature na aktivnost in stabilnost encimskega preparata je pokazala, da ima imobilizirana ChOx višjo toleranco na spremembo pH-vrednosti okolja in višjo termično stabilnost. Prav tako je bila stabilnost imobilizirane ChOx pri ponovni uporabi dobra. V drugem delu doktorske disertacije je opisana priprava aktivnih encimskih skupkov iz encima peroksidaze (HRP, EC 1.11.1.7), pridobljenega iz navadnega hrena (lat.: Armoracia rusticana or Cochlearia armoracia), in postopek zamreženja le-teh z glutaraldehidom za pripravo končne oblike stabilnih zamreženih encimskih skupkov ali na kratko CLEAs. Postopek priprave CLEAs je bil razdeljen na dva ključna dela, in sicer, na obarjanje topnega ali nativnega encima s pomočjo ustreznega obarjalnega reagenta, in na nadaljnjo zamreženje tako izoborjenega encima s pomočjo mrežnega povezovalca. Končni videz CLEAs je podoben motni suspenziji, v kateri so jasno vidni skupki encimov, značilne sferične oblike in premera okrog 250 nm. Uspešno smo sintetizirali zamrežene encimske skupke iz hrenove peroksidaze pod različnimi testnimi pogoji. Končna aktivnost encimskih skupkov iz HRP je bila 83 %. Zamrežene encimske skupke smo sintetizirali v prisotnosti encima albumina iz kokošjih jajc in funkcionalnega aditiva, penta-etilen-heksanamina (PEHA). Encim albumin poveča stabilnost encimskih skupkov, medtem ko PEHA poveča število prostih amino skupin… Advisors/Committee Members: Leitgeb, Maja.

Subjects/Keywords: imobilizacija biokatalizatorja; encimski nosilci; nanostrukturirani materiali; magnetni nanodelci; površinska funkcionalizacija; zamreženi encimski skupki; stabilizacija biokatalizatorja; holesterol oksidaza; hrenova peroksidaza; immobilization of biocatalyst; enzyme supports; nanostructured materials; magnetic nanoparticles; surface functionalization; cross-linked enzyme aggregates; stabilization; cholesterol oxidase; horse radish peroxidase; info:eu-repo/classification/udc/544.473:577.15(043.3)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Šulek, F. (2011). Nanostrukturirani materiali za imobilizacijo biokatalizatorja. (Doctoral Dissertation). Univerza v Mariboru. Retrieved from https://dk.um.si/IzpisGradiva.php?id=18224 ; https://dk.um.si/Dokument.php?id=21662&dn= ; https://plus.si.cobiss.net/opac7/bib/14930966?lang=sl

Chicago Manual of Style (16th Edition):

Šulek, Franja. “Nanostrukturirani materiali za imobilizacijo biokatalizatorja.” 2011. Doctoral Dissertation, Univerza v Mariboru. Accessed May 30, 2020. https://dk.um.si/IzpisGradiva.php?id=18224 ; https://dk.um.si/Dokument.php?id=21662&dn= ; https://plus.si.cobiss.net/opac7/bib/14930966?lang=sl.

MLA Handbook (7th Edition):

Šulek, Franja. “Nanostrukturirani materiali za imobilizacijo biokatalizatorja.” 2011. Web. 30 May 2020.

Vancouver:

Šulek F. Nanostrukturirani materiali za imobilizacijo biokatalizatorja. [Internet] [Doctoral dissertation]. Univerza v Mariboru; 2011. [cited 2020 May 30]. Available from: https://dk.um.si/IzpisGradiva.php?id=18224 ; https://dk.um.si/Dokument.php?id=21662&dn= ; https://plus.si.cobiss.net/opac7/bib/14930966?lang=sl.

Council of Science Editors:

Šulek F. Nanostrukturirani materiali za imobilizacijo biokatalizatorja. [Doctoral Dissertation]. Univerza v Mariboru; 2011. Available from: https://dk.um.si/IzpisGradiva.php?id=18224 ; https://dk.um.si/Dokument.php?id=21662&dn= ; https://plus.si.cobiss.net/opac7/bib/14930966?lang=sl

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