subject:(immobilisation)

Ruhr Universität Bochum

1. Bast, Simon. Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens.

Degree: 2008, Ruhr Universität Bochum

URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-22207

► Fragestellung war, ob BMP-2 im Geflechtknochengewebe der Pars Petrosa des Os Temporale eine knocheninduzierende Wirkung, gleich der im Lamellenknochengewebe, besitzt. Außerdem galt es zu prüfen,… (more)

Subjects/Keywords: Knochen; Mittelohr; Immobilisation; Titan; Implantat

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bast, S. (2008). Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-22207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bast, Simon. “Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens.” 2008. Thesis, Ruhr Universität Bochum. Accessed March 06, 2021. http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-22207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bast, Simon. “Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens.” 2008. Web. 06 Mar 2021.

Vancouver:

Bast S. Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens. [Internet] [Thesis]. Ruhr Universität Bochum; 2008. [cited 2021 Mar 06]. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-22207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bast S. Osteoinduktion durch BMP-2-beschichtete Titan-Implantate im Mittelohr des Kaninchens. [Thesis]. Ruhr Universität Bochum; 2008. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-22207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cranfield University

2. Whitton, Rachel Louise. Algae reactors for wastewater treatment.

Degree: EngD, 2016, Cranfield University

URL: http://dspace.lib.cranfield.ac.uk/handle/1826/10289

► The onset of the Water Framework Directive (WFD) will challenge water utilities to further reduce their wastewater phosphorus discharges to < 0.5 mg.L- 1 .… (more)

Subjects/Keywords: immobilisation; phosphate; nitrogen; circular economy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Whitton, R. L. (2016). Algae reactors for wastewater treatment. (Doctoral Dissertation). Cranfield University. Retrieved from http://dspace.lib.cranfield.ac.uk/handle/1826/10289

Chicago Manual of Style (16^th Edition):

Whitton, Rachel Louise. “Algae reactors for wastewater treatment.” 2016. Doctoral Dissertation, Cranfield University. Accessed March 06, 2021. http://dspace.lib.cranfield.ac.uk/handle/1826/10289.

MLA Handbook (7^th Edition):

Whitton, Rachel Louise. “Algae reactors for wastewater treatment.” 2016. Web. 06 Mar 2021.

Vancouver:

Whitton RL. Algae reactors for wastewater treatment. [Internet] [Doctoral dissertation]. Cranfield University; 2016. [cited 2021 Mar 06]. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10289.

Council of Science Editors:

Whitton RL. Algae reactors for wastewater treatment. [Doctoral Dissertation]. Cranfield University; 2016. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10289

University of Canterbury

3. Raynes, Jared Kenneth. Immobilising biomolecules on amyloid fibrils for biotechnology applications.

Degree: PhD, Biochemistry, 2012, University of Canterbury

URL: http://dx.doi.org/10.26021/9083

► Amyloid fibrils are an insoluble, highly ordered, fibrous protein structure, which have increasingly been recognised as having bionanotechnology applications. Their ability to selfassemble allows a… (more)

▼ Amyloid fibrils are an insoluble, highly ordered, fibrous protein structure, which have increasingly been recognised as having bionanotechnology applications. Their ability to selfassemble allows a bottom-up approach to material design. Their nanometre dimensions affords them a high surface-to-volume ratio and their proteinaceous building blocks from which they are assembled allow for decoration with biomolecules and chemicals through amino acid residues. Amyloid fibrils are therefore a potential nanoscaffold for immobilisation of biomolecules. Immobilisation offers a solution to the problems associated with the use of enzymes in in vitro applications, by increasing their stability, reusability, and in some cases, enhancing catalytic activity. Nanosupports offer a high surface-to-volume ratio compared to classical planar 2-D supports, potentially affording them dramatic increases in immobilisation capacity. To investigate the potential of amyloid fibrils as a novel nanoscaffold, organophosphate hydrolase (OPH), cytochrome P450BM3 (P450BM3), green fluorescent protein (GFP), tobacco etch virus protease (TEV), and glucose oxidase (GOD) were immobilised in solution to the model amyloid fibril forming protein, bovine insulin. Covalently immobilised OPH was found to have a ~300 % increase in relative thermostability at 40 and 50 °C. P450BM3 was not successfully immobilised in its active state, most likely due to unfolding of the enzyme on the amyloid fibril surface. Covalently immobilised GFP retained full fluorescence and acted as a fluorescent protein tag. TEV was shown to have a physical interaction with the nanoscaffold and retain activity. GOD was immobilised and retained activity. Although not all proteins retained activity, a range of different protein structures were successfully immobilised onto the insulin amyloid fibril nanoscaffold. Attachment to the crystallin amyloid fibril nanoscaffold remains a work in progress due to the complexities associated with post-translational modifications of these fibrils. Crystallin amyloid fibrils were assembled on a surface for the first time. Their surface assembled structure was found to resemble spherulites, not previously seen before with crystallin amyloid fibrils. Bovine insulin amyloid fibrils were assembled on the surface of glass beads to increase the available surface area for biomolecule immobilisation. The surface assembled bovine insulin nanoscaffold was first functionalised with GOD, demonstrating that the nanoscaffold provides more surface area for biomolecule immobilisation, although in this case the increase was limited due to high non-specific binding of GOD to the unmodified glass surface. GFP was successfully employed as a fluorescent protein tag to assess the degree of nanoscaffold coverage, confirming the nanoscaffold affords the glass bead a greater surface area. Moreover, a reusable immobilised TEV protease-bead system was developed that was able to sequentially cleave the poly-histidine tags of three different proteins. In conclusion,…

Subjects/Keywords: Amyloid; Immobilisation; Enzymes; Biotechnology; Nanotechnology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Raynes, J. K. (2012). Immobilising biomolecules on amyloid fibrils for biotechnology applications. (Doctoral Dissertation). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/9083

Chicago Manual of Style (16^th Edition):

Raynes, Jared Kenneth. “Immobilising biomolecules on amyloid fibrils for biotechnology applications.” 2012. Doctoral Dissertation, University of Canterbury. Accessed March 06, 2021. http://dx.doi.org/10.26021/9083.

MLA Handbook (7^th Edition):

Raynes, Jared Kenneth. “Immobilising biomolecules on amyloid fibrils for biotechnology applications.” 2012. Web. 06 Mar 2021.

Vancouver:

Raynes JK. Immobilising biomolecules on amyloid fibrils for biotechnology applications. [Internet] [Doctoral dissertation]. University of Canterbury; 2012. [cited 2021 Mar 06]. Available from: http://dx.doi.org/10.26021/9083.

Council of Science Editors:

Raynes JK. Immobilising biomolecules on amyloid fibrils for biotechnology applications. [Doctoral Dissertation]. University of Canterbury; 2012. Available from: http://dx.doi.org/10.26021/9083

University of Sydney

4. Olsson, Annabelle. Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles .

Degree: 2013, University of Sydney

URL: http://hdl.handle.net/2123/10446

► The objective of this research was to determine a set of commonly available chemical restraint options which produced safe, reliable and repeatable immobilisation for estuarine… (more)

Subjects/Keywords: Crocodile; Immobilisation; Medetomidine; Alfaxalone; Midazolam

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Olsson, A. (2013). Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/10446

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Olsson, Annabelle. “Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles .” 2013. Thesis, University of Sydney. Accessed March 06, 2021. http://hdl.handle.net/2123/10446.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Olsson, Annabelle. “Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles .” 2013. Web. 06 Mar 2021.

Vancouver:

Olsson A. Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles . [Internet] [Thesis]. University of Sydney; 2013. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2123/10446.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Olsson A. Investigation into developing reliable immobilisation protocol for use under varying environmental temperatures in estuarine and Australian freshwater crocodiles . [Thesis]. University of Sydney; 2013. Available from: http://hdl.handle.net/2123/10446

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Limerick

5. Abdallah, Noreldeen Hassan. Immobilisation of catalysts for applications in organic reactions.

Degree: 2016, University of Limerick

URL: http://hdl.handle.net/10344/5219

►

peer-reviewed

The use of catalysts generally requires the immobilisation of the catalyst at specific locations, a requirement that can pose a significant challenge. The use… (more)

▼

peer-reviewed

The use of catalysts generally requires the immobilisation of the catalyst at specific locations, a requirement that can pose a significant challenge. The use of mesoporous silicate materials as supports offers some valuable advantages and can provide a more stable environment for catalysts. In this instance, this project was focused on the preparation, characterisation and optimization of immobilized catalysts (enzyme and metal based) for applications in organic reactions. The goal is to obtain an optimal catalytic performance combined with long-term operational stability. In order to achieve this goal, a detailed understanding of the solid supports, the catalysts, and of the immobilization process is essential. Cytochrome c and Candida antartica lipase B (CALB) were immobilised on to the mesoporous silicate SBA-15 and a porous spherical silicate material (PPS) in order to determine the influence of differences in the properties of supports such as pore volume, surface area and morphology on factors such as loading, activity and stability of enzymes. The catalytic activity of cytochrome c was similar on both supports, while the loading and catalytic activity of CALB on SBA-15 was higher when compared to PPS. These results indicate that the amount of adsorbed enzyme and activity are a reflection of the different surface areas, pore shapes and pore volumes of the two materials. Leaching of the enzyme from the support can result in substantial losses in activity, particularly on reuse. To overcome this disadvantage, it is necessary to utilise surface modification of the silica support to specifically bind the immobilised enzyme. In order to explore this effect, covalent attachment of Nickel/Cobalt-cyclam onto MCF were examined. The amount of cyclam modified onto the surface of MCF increased as the amounts of starting materials were increased. Ni/Co-cyclam modified materials were tested for their ability to immobilise His6-alanine racemase (HT-AlaR). The catalytic activity of immobilized HT-AlaR was comparable to the free enzyme and also maintained its activity after 5 cycles. After successfully immobilising cytochrome c, lipase CALB and HT-AlaR on silicate materials by physical adsorption and covalent methods, the next step was to utilize these methods to immobilize glucose oxidase. Numerous experiments were performed to immobilise glucose oxidase. The aim was to prepare highly active and stable biocatalysts which have capability to work in extreme reaction conditions. After examined immobilisation of GOx using physical adsorption, covalent bonding and cross linking methods. GOx immobilized by cross linking methods offers many advantages such as high activity and operational stability over other methods. The synthesis and characterisation of the manganese (Mn) complex 4,7-dimethyl-1,4,7-triazacyclonone (DMTACN) immobilised on mesoporous silica was performed. FT-IR, Elemental analysis and X-Ray photoelectron spectra indicated the presence of catalytic species in an active conformation. Under the…

Advisors/Committee Members: Magner, Edmond.

Subjects/Keywords: chemistry; immobilisation of the catalyst

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abdallah, N. H. (2016). Immobilisation of catalysts for applications in organic reactions. (Thesis). University of Limerick. Retrieved from http://hdl.handle.net/10344/5219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abdallah, Noreldeen Hassan. “Immobilisation of catalysts for applications in organic reactions.” 2016. Thesis, University of Limerick. Accessed March 06, 2021. http://hdl.handle.net/10344/5219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abdallah, Noreldeen Hassan. “Immobilisation of catalysts for applications in organic reactions.” 2016. Web. 06 Mar 2021.

Vancouver:

Abdallah NH. Immobilisation of catalysts for applications in organic reactions. [Internet] [Thesis]. University of Limerick; 2016. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10344/5219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abdallah NH. Immobilisation of catalysts for applications in organic reactions. [Thesis]. University of Limerick; 2016. Available from: http://hdl.handle.net/10344/5219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oulu

6. Koivukangas, A. (Antti). Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects.

Degree: 2002, University of Oulu

URL: http://urn.fi/urn:isbn:9514267052

► Abstract Bisphosphonates (BPs) are used in the treatment of osteoporosis. However, their effects, especially long-term effects, on bone and bone healing are not fully known.… (more)

▼ Abstract Bisphosphonates (BPs) are used in the treatment of osteoporosis. However, their effects, especially long-term effects, on bone and bone healing are not fully known. Clodronate (dichloromethylene bisphosphonic acid) is a first-generation BP. The thesis was based on two animal experiments. The first, with 199 rats on long-term clodronate treatment, was divided into four separate substudies. The effects of long-term administration of clodronate to rats were investigated after 32 weeks of treatment. The effects on the femoral shaft, femoral neck and vertebra in normal, non-osteoporotic skeleton were described in two publications. The evaluations were made by biomechanical, densitometric, histological, hematological and electron-microscopic investigations. Fracture healing was investigated in rats after 24 weeks of clodronate treatment. The tibia was fractured, and the effects of treatments were evaluated at 4 and 8 weeks after the fracture. Radiographs and densitometric pQCT in the evaluation of experimental fracture healing were compared. In the other experiment with 30 mice, a mouse immobilisation osteoporosis model for further studies was investigated. Long-term administration of clodronate at therapeutic dosage had no harmful impacts but rather some beneficial effects on normal, non-osteoporotic bone. However, long-term high-dose clodronate treatment resulted in a decrement of tibial length but did not have any other significant or adverse effects. In the evaluation of fracture healing, pQCT proved to be better than radiographs in differentiating the total mineralised cross-sectional area of callus and the area of compact bone. Clodronate treatment does not seem to prolong the fracture healing process, even when administered on a long-term basis before the fracture. Clodronate increased the size of callus, but had only a minor effect on its biomechanical properties. Three weeks of hind limb immobilization caused local osteopenia in the tibia when compared to its contralateral leg. In conclusion, this thesis suggests that long-term administration of clodronate at therapeutic dosage has no harmful, but rather some beneficial effects on normal, non-osteoporotic bone. However, a fivefold dose of clodronate causes a slight decrease in the growth of tibial length. Healing of fractures during or after clodronate treatment is not inhibited.

Subjects/Keywords: bisphosphonate; immobilisation; osteoporosis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Koivukangas, A. (. (2002). Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9514267052

Chicago Manual of Style (16^th Edition):

Koivukangas, A (Antti). “Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects.” 2002. Doctoral Dissertation, University of Oulu. Accessed March 06, 2021. http://urn.fi/urn:isbn:9514267052.

MLA Handbook (7^th Edition):

Koivukangas, A (Antti). “Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects.” 2002. Web. 06 Mar 2021.

Vancouver:

Koivukangas A(. Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects. [Internet] [Doctoral dissertation]. University of Oulu; 2002. [cited 2021 Mar 06]. Available from: http://urn.fi/urn:isbn:9514267052.

Council of Science Editors:

Koivukangas A(. Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects. [Doctoral Dissertation]. University of Oulu; 2002. Available from: http://urn.fi/urn:isbn:9514267052

Cranfield University

7. Whitton, Rachel Louise. Algae reactors for wastewater treatment.

Degree: Thesis (D.Eng.), 2016, Cranfield University

URL: http://dspace.lib.cranfield.ac.uk/handle/1826/10289 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.691036

► The onset of the Water Framework Directive (WFD) will challenge water utilities to further reduce their wastewater phosphorus discharges to < 0.5 mg.L- 1. Whilst… (more)

Subjects/Keywords: 628.3; immobilisation; phosphate; nitrogen; circular economy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Whitton, R. L. (2016). Algae reactors for wastewater treatment. (Doctoral Dissertation). Cranfield University. Retrieved from http://dspace.lib.cranfield.ac.uk/handle/1826/10289 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.691036

Chicago Manual of Style (16^th Edition):

Whitton, Rachel Louise. “Algae reactors for wastewater treatment.” 2016. Doctoral Dissertation, Cranfield University. Accessed March 06, 2021. http://dspace.lib.cranfield.ac.uk/handle/1826/10289 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.691036.

MLA Handbook (7^th Edition):

Whitton, Rachel Louise. “Algae reactors for wastewater treatment.” 2016. Web. 06 Mar 2021.

Vancouver:

Whitton RL. Algae reactors for wastewater treatment. [Internet] [Doctoral dissertation]. Cranfield University; 2016. [cited 2021 Mar 06]. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10289 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.691036.

Council of Science Editors:

Whitton RL. Algae reactors for wastewater treatment. [Doctoral Dissertation]. Cranfield University; 2016. Available from: http://dspace.lib.cranfield.ac.uk/handle/1826/10289 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.691036

Luleå University of Technology

8. Niero, Luisa. Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments.

Degree: 2013, Luleå University of Technology

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42430

►

The aim of this study was to assess the effectiveness of chemical stabilization technique on arsenic (As) contaminated soil amended with metallic or zerovalent… (more)

Subjects/Keywords: Technology; Soil remediation; immobilisation; chemical stabilisation; Teknik

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Niero, L. (2013). Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments. (Thesis). Luleå University of Technology. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Niero, Luisa. “Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments.” 2013. Thesis, Luleå University of Technology. Accessed March 06, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Niero, Luisa. “Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments.” 2013. Web. 06 Mar 2021.

Vancouver:

Niero L. Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments. [Internet] [Thesis]. Luleå University of Technology; 2013. [cited 2021 Mar 06]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Niero L. Assessment of Arsenic Stability in Iron Amended Soil by Laboratory and Field Experiments. [Thesis]. Luleå University of Technology; 2013. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-42430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Ben Osman, Chirine. Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate.

Degree: Docteur es, Physique et Chimie des Matériaux, 2014, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2014PA066564

►

L’objectif était de développer et d’utiliser des nanoparticules hybrides de type«coeur-écorce» composées d’un coeur de silice dense entouré par des chaînes polymères fonctionnalisées par des… (more)

▼

L’objectif était de développer et d’utiliser des nanoparticules hybrides de type«coeur-écorce» composées d’un coeur de silice dense entouré par des chaînes polymères fonctionnalisées par des ligands d’ATRP comme support catalytique pour la polymérisation du MMA par ATRP. Deux stratégies de synthèse de nanoparticules hybrides ont été élaborées. La première a consisté à immobiliser sur un support de silice, d’une manière covalente, des chaînes fonctionnelles synthétisées par NMP comportant des ligands capables de complexer le bromure de cuivre. Des nanoparticules de [email protected]ère fonctionnel ayant de faibles densités de greffage en polymère ont été ciblées afin d’éviter leur recouvrement sur la surface. Cependant, les polymérisations n’étaient pas contrôlées probablement à cause d’un manque d’accessibilité de l’amorceur et des radicaux propageants aux complexes decuivre. Afin de rendre les catalyseurs supportés plus accessibles en solution, nous avons élaboré une seconde stratégie basée sur un système catalytique hybride réversible. Il consiste à immobiliser des chaînes α-fonctionnalisées par un motif donneur-accepteur de proton(DAD) via des liaisons hydrogène sur des particules de silice modifiées par un motif complémentaire (ADA). Des PMMA de masses molaires contrôlées ont été synthétisés avec des dispersités plus faibles que celles obtenues en ATRP homogène (en présence des chaînes libres). Après séparation du catalyseur du milieu réactionnel par centrifugation, nous avons montré qu’avec un excès du motif ADA, 96% du cuivre initialement introduit ont été récupérés.

The aim of this project is to develop hybrid nanoparticles bearing well definedpolymer arms as supported catalyst for the atom transfer radical polymerization of methylmethacrylate. This new generation of “semi heterogeneous" catalysts was prepared by twostrategies. The first consisted of immobilizing the polymer arms bearing the ligands enablingcoordination of copper bromide onto silica particles by covalent bonds. Hybrid nanoparticleswith low polymer grafting density were targeted to prevent the overlapping of chains on thesurface. Unfortunately, the polymerizations were not controlled probably due to a lack ofaccessibility of the initiator and propagating radicals to the copper complexes. To improve theaccessibility, a reversibly supported catalyst was developed via self-assembly using hydrogenbonding between chains α-functionalized by a proton donor-acceptor unit (DAD) and acomplementary unit (ADA) anchored on silica particles. These new hybrid materials wereefficient in the controlled radical polymerization of MMA, yielding polymers with controlledmolecular weights and dispersities narrower than those obtained for homogeneous ATRP.Moreover, after catalyst separation from the reaction medium by centrifugation, more than96% of the originally used copper was recovered.

Advisors/Committee Members: Fradet, Alain (thesis director).

Subjects/Keywords: Atrp; Catalyseur supporté; Immobilisation covalente; Immobilisation réversible; Nanoparticules hybrides; Polymères fonctionnalisés; Silice Stöber; ATRP; Supported catalyst; 547.8

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ben Osman, C. (2014). Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2014PA066564

Chicago Manual of Style (16^th Edition):

Ben Osman, Chirine. “Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate.” 2014. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 06, 2021. http://www.theses.fr/2014PA066564.

MLA Handbook (7^th Edition):

Ben Osman, Chirine. “Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate.” 2014. Web. 06 Mar 2021.

Vancouver:

Ben Osman C. Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2014PA066564.

Council of Science Editors:

Ben Osman C. Synthèse et utilisation de nouveaux matériaux hybrides pour la catalyse en ATRP supportée du méthacrylate de méthyle : Synthesis of hybrid nanoparticles containing a supported catalyst and its use in the atom transfer radical polymerization of methyl methacrylate. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2014. Available from: http://www.theses.fr/2014PA066564

University of Pretoria

10. [No author]. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase .

Degree: 2013, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-07162013-123720/

► Wild animal capture has progressed over the years from trapping or physical capture, which was dangerous to both animal and man, to chemical immobilization. Opioids… (more)

▼ Wild animal capture has progressed over the years from trapping or physical capture, which was dangerous to both animal and man, to chemical immobilization. Opioids and butyrophenones are the most common classes of drugs used for ungulate immobilization; however newer drugs and drug combinations are commonly used in an attempt to reduce time to immobilization in wildlife. The enzyme hyaluronidase is often added to drug combinations in the belief that it reduces time to immobilization by improving drug absorption. The primary objective of this study was to ascertain if the addition of hyaluronidase to an etorphine and azaperone drug combination would be of value in reducing time to immobilization in blue wildebeest. The study also tried to ascertain if the added hyaluronidase enabled one to reduce the etorphine and azaperone doses required to immobilize blue wildebeest, without affecting time to immobilization. The study made use of a four-way cross-over study design, with four treatment groups, four sequences and four periods. The four treatment groups were etorphine and azaperone; etorphine, azaperone and 5000 international units (IU) hyaluronidase; etorphine, azaperone and 7500 IU hyaluronidase; and 75 % of the original etorphine dose, 75% of the original azaperone dose and 7500 IU hyaluronidase. Each animal was immobilized with each of the above four drug combinations randomly over an eight week period with a two week interval between each period. The times to first effect, first down and immobilization were recorded. The etorphine and azaperone treatment group was used as the control group. The difference in time to first effect between the control group and the etorphine, azaperone and 7500 IU hyaluronidase treatment group was statistically significant (95 seconds versus 67 seconds; p = 0.007). When compared to the time to immobilization in the control group (323 seconds) the time to immobilization in the etrophine, azaperone and 5000 IU hyaluronidase (228 seconds); etorphine, azaperone and 7500 IU hyaluronidase (210 seconds) and the low dose etorphine, low dose azaperone and 7500 IU hyaluronidase (268 seconds) groups were statistically significantly reduced (p=0.002, p=0.001 and p=0.045 respectively). It is therefore concluded that the addition of 5000 or 7500 IU hyaluronidase to an etorphine and azaperone combination significantly reduced the time to immobilization in blue wildebeest. The unexpected decrease in time to immobilization in the low dose etorphine, low dose azaperone and 7500 IU hyaluronidase treatment group requires further investigation. Advisors/Committee Members: Dr L Venter (advisor), Prof V Naidoo (advisor).

Subjects/Keywords: Connochaetes taurinus; Blue wildebeest; Drug combinations; Azaperone; Hyaluronidase; Etorphine; Immobilisation; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2013). Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07162013-123720/

Chicago Manual of Style (16^th Edition):

author], [No. “Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase .” 2013. Masters Thesis, University of Pretoria. Accessed March 06, 2021. http://upetd.up.ac.za/thesis/available/etd-07162013-123720/.

MLA Handbook (7^th Edition):

author], [No. “Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase .” 2013. Web. 06 Mar 2021.

Vancouver:

author] [. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase . [Internet] [Masters thesis]. University of Pretoria; 2013. [cited 2021 Mar 06]. Available from: http://upetd.up.ac.za/thesis/available/etd-07162013-123720/.

Council of Science Editors:

author] [. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes Taurinus) by the addition of hyaluronidase . [Masters Thesis]. University of Pretoria; 2013. Available from: http://upetd.up.ac.za/thesis/available/etd-07162013-123720/

11. M.P, Saravanakumar. Immobilisation of cr6 in contaminated soils using neem bark powder; -.

Degree: Mechanical and building science, 2014, VIT University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/37850

Abstract available

Reference and publication givan

Advisors/Committee Members: Phanikumar, B R.

Subjects/Keywords: Contaminated; Immobilisation; Powder; Soils

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

M.P, S. (2014). Immobilisation of cr6 in contaminated soils using neem bark powder; -. (Thesis). VIT University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/37850

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

M.P, Saravanakumar. “Immobilisation of cr6 in contaminated soils using neem bark powder; -.” 2014. Thesis, VIT University. Accessed March 06, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/37850.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

M.P, Saravanakumar. “Immobilisation of cr6 in contaminated soils using neem bark powder; -.” 2014. Web. 06 Mar 2021.

Vancouver:

M.P S. Immobilisation of cr6 in contaminated soils using neem bark powder; -. [Internet] [Thesis]. VIT University; 2014. [cited 2021 Mar 06]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/37850.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

M.P S. Immobilisation of cr6 in contaminated soils using neem bark powder; -. [Thesis]. VIT University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/37850

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

12. Pretorius, Chantelle. Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk.

Degree: MSc, Microbiology, 2012, Stellenbosch University

URL: http://hdl.handle.net/10019.1/71958

►

ENGLISH ABSTRACT: ß-Galactosidase of Escherichia coli is the equivalent of lactase in humans and has the ability to bind and hydrolyse lactose. Lactase de ciency… (more)

▼

ENGLISH ABSTRACT: ß-Galactosidase of Escherichia coli is the equivalent of lactase in humans and has the ability to bind and hydrolyse lactose. Lactase de ciency is a common phenomenon present in almost 70% of the world's population. This has resulted in greater than before demands on the food processing industry to develop a method that will allow for the hydrolysis of the disaccharide lactose in milk but will also allow for the removal of the remaining active enzyme. In this thesis, a new method, that is bio-speci c and well characterized for the removal of lactose from a lactose containing solution, is described. The E537D mutated version of ß-Galactosidase, which has a much lower activity compared to the wildtype and is able to bio-speci cally bind lactose for longer periods, was covalently immobilised to commercially available magnetic nanoparticles (fl uidMAG-Amine) via two coupling strategies. Glutaraldehyde is a cross-linking agent that reacts with amine groups, while N- (3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) is a coupling agent that activates carboxylic groups. These agents are widely used for the coupling of biomolecules to solid supports. The covalently coupled fluidMAG-E537D ß-Galactosidase particles were characterized regarding retained enzymatic activity and ability to bind and physically remove lactose from a lactose containing solution by applying an external magnetic eld, after lactose binding, to the enzyme-particle complex in solution. Each component aimed at yielding this functionally immobilised enzyme complex was studied and optimized to contribute to the development of this novel technique, which is a ordable and simple, for the removal of lactose from solution for the ultimate production of lactose free milk. Results indicated the glutaraldehyde method of ß-Gal cross-linking to fluidMAG-Amine to be the preferred strategy since it allowed an increased carrier capacity of protein to the particles. The glutaraldehyde cross-linked protein also exhibited a two-fold higher activity than the EDC coupled protein. Furthermore, the glutaraldehyde cross-linked fluidMAG-E537D ß-Gal was able to physically remove 34 % of the lactose from a 0.2 nmol/L lactose in solution. This, therefore, con rmed the potential use of this novel technique in the food processing industry.

AFRIKAANSE OPSOMMING: ß-Galaktosidase vanaf Escherichia coli is dieselfde as laktase in mense en beskik oor die vermoë om laktose te bind en te hidroliseer. 'n Gebrek aan laktase kom algemeen voor en ongeveer 70 % van die wêreldbevolking ly hieraan. Laasgenoemde het daartoe gelei dat daar meer druk as vantevore op die voedselproduksie industrie is om 'n metode te ontwikkel waarmee die hidrolise van die disakkaried laktose in melk moontlik sal wees asook die verwydering van die oorblywende aktiewe ensiem. In hierdie tesis word 'n nuwe metode beskryf wat biospesi ek en goed gekarakteriseer is vir die verwydering van laktose vanuit 'n laktose bevattende oplossing.…

Advisors/Committee Members: Swart, Pieter, Storbeck, Karl-Heinz, Stellenbosch University. Faculty of Science. Dept. of Microbiology..

Subjects/Keywords: Beta-Galactosidase – Covalent immobilisation; Escherichia coli; Magnetic nanoparticles; Lactose; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pretorius, C. (2012). Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/71958

Chicago Manual of Style (16^th Edition):

Pretorius, Chantelle. “Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk.” 2012. Masters Thesis, Stellenbosch University. Accessed March 06, 2021. http://hdl.handle.net/10019.1/71958.

MLA Handbook (7^th Edition):

Pretorius, Chantelle. “Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk.” 2012. Web. 06 Mar 2021.

Vancouver:

Pretorius C. Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk. [Internet] [Masters thesis]. Stellenbosch University; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10019.1/71958.

Council of Science Editors:

Pretorius C. Covalent immobilisation of β-Galactosidase from Escherichia coli to commercially available magnetic nanoparticles for the removal of lactose from milk. [Masters Thesis]. Stellenbosch University; 2012. Available from: http://hdl.handle.net/10019.1/71958

13. Panda, Chittaranjan. Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -.

Degree: Engineering and technology, 2014, Shiksha o Anusandhan University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/43103

Abstract available

Bibliography p.173-187 and publication given

Advisors/Committee Members: Mishra, Kamala Kanta and Nayak, Bansidhara.

Subjects/Keywords: Immobilisation; Material; Mechanism; Study

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Panda, C. (2014). Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -. (Thesis). Shiksha o Anusandhan University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/43103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Panda, Chittaranjan. “Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -.” 2014. Thesis, Shiksha o Anusandhan University. Accessed March 06, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/43103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Panda, Chittaranjan. “Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -.” 2014. Web. 06 Mar 2021.

Vancouver:

Panda C. Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -. [Internet] [Thesis]. Shiksha o Anusandhan University; 2014. [cited 2021 Mar 06]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/43103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Panda C. Study of chromium leaching and its immobilisation mechanism in the assessment of ferrochrome slag as concrete aggregate material; -. [Thesis]. Shiksha o Anusandhan University; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/43103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

14. Dittberner, Mark. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase.

Degree: Production Animal Studies, 2011, University of Pretoria

URL: http://hdl.handle.net/2263/26364

► Wild animal capture has progressed over the years from trapping or physical capture, which was dangerous to both animal and man, to chemical immobilization. Opioids… (more)

▼ Wild animal capture has progressed over the years from trapping or physical capture, which was dangerous to both animal and man, to chemical immobilization. Opioids and butyrophenones are the most common classes of drugs used for ungulate immobilization; however newer drugs and drug combinations are commonly used in an attempt to reduce time to immobilization in wildlife. The enzyme hyaluronidase is often added to drug combinations in the belief that it reduces time to immobilization by improving drug absorption. The primary objective of this study was to ascertain if the addition of hyaluronidase to an etorphine and azaperone drug combination would be of value in reducing time to immobilization in blue wildebeest. The study also tried to ascertain if the added hyaluronidase enabled one to reduce the etorphine and azaperone doses required to immobilize blue wildebeest, without affecting time to immobilization. The study made use of a four-way cross-over study design, with four treatment groups, four sequences and four periods. The four treatment groups were etorphine and azaperone; etorphine, azaperone and 5000 international units (IU) hyaluronidase; etorphine, azaperone and 7500 IU hyaluronidase; and 75 % of the original etorphine dose, 75% of the original azaperone dose and 7500 IU hyaluronidase. Each animal was immobilized with each of the above four drug combinations randomly over an eight week period with a two week interval between each period. The times to first effect, first down and immobilization were recorded. The etorphine and azaperone treatment group was used as the control group. The difference in time to first effect between the control group and the etorphine, azaperone and 7500 IU hyaluronidase treatment group was statistically significant (95 seconds versus 67 seconds; p = 0.007). When compared to the time to immobilization in the control group (323 seconds) the time to immobilization in the etrophine, azaperone and 5000 IU hyaluronidase (228 seconds); etorphine, azaperone and 7500 IU hyaluronidase (210 seconds) and the low dose etorphine, low dose azaperone and 7500 IU hyaluronidase (268 seconds) groups were statistically significantly reduced (p=0.002, p=0.001 and p=0.045 respectively). It is therefore concluded that the addition of 5000 or 7500 IU hyaluronidase to an etorphine and azaperone combination significantly reduced the time to immobilization in blue wildebeest. The unexpected decrease in time to immobilization in the low dose etorphine, low dose azaperone and 7500 IU hyaluronidase treatment group requires further investigation. Advisors/Committee Members: Venter, Linda (advisor), Naidoo, Vinny (advisor).

Subjects/Keywords: Connochaetes taurinus; Blue wildebeest; Drug combinations; Azaperone; Hyaluronidase; Etorphine; Immobilisation; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dittberner, M. (2011). Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26364

Chicago Manual of Style (16^th Edition):

Dittberner, Mark. “Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase.” 2011. Masters Thesis, University of Pretoria. Accessed March 06, 2021. http://hdl.handle.net/2263/26364.

MLA Handbook (7^th Edition):

Dittberner, Mark. “Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase.” 2011. Web. 06 Mar 2021.

Vancouver:

Dittberner M. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase. [Internet] [Masters thesis]. University of Pretoria; 2011. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2263/26364.

Council of Science Editors:

Dittberner M. Accelerated induction of etorphine immobilization in blue wildebeest (Connochaetes taurinus) by the addition of hyaluronidase. [Masters Thesis]. University of Pretoria; 2011. Available from: http://hdl.handle.net/2263/26364

University of Adelaide

15. Maddigan, Natasha Kate. Enzyme Immobilisation Using Porous Frameworks.

Degree: 2020, University of Adelaide

URL: http://hdl.handle.net/2440/126087

► Enzymes are often sought after for applications in industry and synthetic chemistry due to their high reactivity and substrate selectivity, often surpassing their chemical counterparts.… (more)

▼ Enzymes are often sought after for applications in industry and synthetic chemistry due to their high reactivity and substrate selectivity, often surpassing their chemical counterparts. They are, however, limited by their structural instability and require restrictive environmental conditions that are often not compatible with industrial processing. As such, new technologies are required to protect enzymes from non-biological conditions. This thesis investigates enzyme immobilisation using porous frameworks including metal-organic frameworks (MOFs) and hydrogen-bonded organic frameworks (HOFs). The diverse nature of both the enzyme and MOF/HOF components offers great potential for creating a broad library of biocomposites with novel function. There are however inherent challenges in finding appropriate conditions for immobilisation in which the enzyme remains active, and where the overall biocomposite is stable. Initial studies utilised Zeolitic Imidazolate Framework 8 (ZIF-8), a subclass of MOFs, for protein immobilisation. The addition of biomacromolecules, such as proteins, can promote the self-assembly of ZIF-8 by a process known as “biomimetic mineralisation”. Systematic screening studies established that this process is biomacromolecule dependent, with a subset of proteins requiring the addition of organic solvent or increased ligand concentrations to promote ZIF-8 nucleation. These reaction conditions were also instrumental in controlling the topology, morphology, and particle size of the biocomposites. Investigations into the influence of the protein revealed that biomimetic mineralisation is governed by the surface chemistry of the biomacromolecules, with a more negative surface charge promoting rapid nucleation, resulting from enhanced zinc ion concentration at the surface. Chemical functionalisation can be implemented, to alter the electrostatic potential of the protein surface and control the biomimetic mineralisation process. The biocomposites from different immobilisation strategies for ZIF-8 were assessed for biocatalytic activity using two distinct enzymes, a lipase, and a dehalogenase. The activity was analysed relative to the free enzyme to interrogate the impact of immobilisation on the function and stability of the biocatalyst. Variation in support stability and biocomposite activity were observed. Each were dependent on the method of immobilisation with some strategies yielding inactive or unstable biocomposites. For lipase, the ZIF-8 framework provided enzymatic stability to organic solvent, whilst the framework itself was susceptible to degradation by phosphate buffer. In the case of the dehalogenase biocomposite, substrate dependent crystal degradation was observed that was deemed responsible for variations in the observed enzyme activity. These findings highlight the potential limitations of ZIF-8 for enzyme immobilisation and as such, alternative porous supports were targeted. Framework chemistry and porosity were further investigated utilising Zeolitic Imidazolate Framework-90 (ZIF-90) and… Advisors/Committee Members: Bell, Stephen (advisor), Doonan, Christian (advisor), School of Physical Sciences (school).

Subjects/Keywords: enzyme immobilisation; metal-organic framework; hydrogen-bonded organic framework .; biocatalysis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maddigan, N. K. (2020). Enzyme Immobilisation Using Porous Frameworks. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/126087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maddigan, Natasha Kate. “Enzyme Immobilisation Using Porous Frameworks.” 2020. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/126087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maddigan, Natasha Kate. “Enzyme Immobilisation Using Porous Frameworks.” 2020. Web. 06 Mar 2021.

Vancouver:

Maddigan NK. Enzyme Immobilisation Using Porous Frameworks. [Internet] [Thesis]. University of Adelaide; 2020. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/126087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maddigan NK. Enzyme Immobilisation Using Porous Frameworks. [Thesis]. University of Adelaide; 2020. Available from: http://hdl.handle.net/2440/126087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Montpellier II

16. Techer, Vincent. Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells.

Degree: Docteur es, Chimie et physicochimie des matériaux, 2013, Université Montpellier II

URL: http://www.theses.fr/2013MON20166

►

Les biopiles enzymatiques sont considérés comme des systèmes potentiellement utilisables pour la production d'énergie renouvelable dans des marchés niches. Une biopile est constituée de deux… (more)

▼

Les biopiles enzymatiques sont considérés comme des systèmes potentiellement utilisables pour la production d'énergie renouvelable dans des marchés niches. Une biopile est constituée de deux électrodes associées à des enzymes, catalyseurs biologiques, qui permettent la production d'énergie électrique à partir de réactions chimiques d'oxydoréduction. Ce travail présente la réalisation d'une biopile alcool/oxygène, au sein de laquelle l'alcool est oxydé à l'anode par l'alcool déshydrogénase alors que l'oxygène moléculaire est réduit en eau à la cathode par l'enzyme laccase, en présence de médiateurs spécifiques. L'objectif de ce travail a été tout d'abord de développer des bioélectrodes avec des enzymes immobilisées de manière à minimiser la quantité de biocatalyseur et augmenter sa stabilité. Dans un second temps, l'assemblage de biocathodes et de bioanodes a permis de fabriquer des biopiles à alcool macroscopique et microfluidique. Différentes poudres de carbone combinées à des polymères ont été utilisées pour immobiliser les enzymes et les médiateurs par encapsulation selon diverses configurations. Des analyses électrochimiques ont permis de mettre en évidence l'influence importante de certains paramètres comme la nature du carbone et du polymère, le pH et la température sur les performances des bioélectrodes. Une fois assemblées dans les configurations classique ou microfluidique, ces bioélectrodes ont conduit à des systèmes électrochimiques de génération d'énergie délivrant une densité de puissance maximale de 300μW/cm2 à 0,61V pour la biopile macroscopique et de 45μW/cm2 à 0,5V pour le système microfluidique.

Enzymatic biofuel cells (BFC) are systems of great interest for the production of renewable energy in niche markets. A BFC consists of two electrodes associated with enzymes as catalysts allowing energy production from oxydoreduction reactions. This work is devoted to the development of an alcohol/oxygen BFC for which alcohol is oxidized at the anode by alcohol dehydrogenase while molecular oxygen is reduced to water at the cathode by laccase, in the presence of specific mediators. The objective of this work was first to develop bioelectrodes with immobilized enzymes in order to minimize the amount of biocatalyst and increase its stability. In a second step, biocathodes and bioanodes were assembled to make macroscopic and microfluidic alcohol BFCs. Various carbon powders combined to polymers were used to immobilize enzymes and mediators in various configurations by entrapment. Electrochemical analysis have highlighted the significant influence of certain parameters like the nature of polymer and carbon, the pH or the temperature on the bioelectrodes performances. Once assembled in classical or microfluidic configurations, these bioelectrode led to electrochemical energy generation systems delivering a maximum power density of 300μW/cm2 at 0,61V for the macroscopic BFC and 45μW/cm2 at 0,5V for the microfluidic system.

Advisors/Committee Members: Tingry, Sophie (thesis director), Cretin, Marc (thesis director).

Subjects/Keywords: Biopile; Microfluidique; Enzymes; Immobilisation; Biofuel cell; Microfluidic; Enzymes; Immobilization

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Techer, V. (2013). Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2013MON20166

Chicago Manual of Style (16^th Edition):

Techer, Vincent. “Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells.” 2013. Doctoral Dissertation, Université Montpellier II. Accessed March 06, 2021. http://www.theses.fr/2013MON20166.

MLA Handbook (7^th Edition):

Techer, Vincent. “Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells.” 2013. Web. 06 Mar 2021.

Vancouver:

Techer V. Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells. [Internet] [Doctoral dissertation]. Université Montpellier II; 2013. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2013MON20166.

Council of Science Editors:

Techer V. Electrodes catalytiques à base d’enzymes pour le développement de biopiles alcool/oxygène microfluidiques. : Catalytic electrodes based on enzymes for the development of microfluidic alcohol/oxygen biofuel cells. [Doctoral Dissertation]. Université Montpellier II; 2013. Available from: http://www.theses.fr/2013MON20166

Queensland University of Technology

17. Huang, Yiming. Synthesis and modification of Alumina nanofibres and its applications.

Degree: 2013, Queensland University of Technology

URL: https://eprints.qut.edu.au/62446/

► This thesis is a forward study of alumina nanofiber material in developing its applications biology field. It demonstrates that by applying proper modification strategy, alumina… (more)

Subjects/Keywords: Boehmite nanofibers; γ-Alumina membrane; Protein separation; Laccase immobilisation; Surface modification

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, Y. (2013). Synthesis and modification of Alumina nanofibres and its applications. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/62446/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Yiming. “Synthesis and modification of Alumina nanofibres and its applications.” 2013. Thesis, Queensland University of Technology. Accessed March 06, 2021. https://eprints.qut.edu.au/62446/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Yiming. “Synthesis and modification of Alumina nanofibres and its applications.” 2013. Web. 06 Mar 2021.

Vancouver:

Huang Y. Synthesis and modification of Alumina nanofibres and its applications. [Internet] [Thesis]. Queensland University of Technology; 2013. [cited 2021 Mar 06]. Available from: https://eprints.qut.edu.au/62446/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang Y. Synthesis and modification of Alumina nanofibres and its applications. [Thesis]. Queensland University of Technology; 2013. Available from: https://eprints.qut.edu.au/62446/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

18. Egan, Sally. Suitability of energy cropping on abandoned metal contaminated farmland.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/77087

► A great deal of research is being undertaken globally on the viability of alternative energy sources given the potential effects of climate change and increases… (more)

▼ A great deal of research is being undertaken globally on the viability of alternative energy sources given the potential effects of climate change and increases in oil prices. Energy cropping is one of many emerging technologies for producing renewable energy that is being widely researched around the world, and is in fullscale production in some countries. Abandoned contaminated land, awaiting remediation is another major global problem. This masters thesis studies the potential re-use of abandoned metal contaminated land for growing ‘energy crops’ at a field site in China. Thus, with a potential to not only find an economical way to re-use and rehabilitate contaminated sites, but also to produce potentially ‘carbon neutral’ renewable sources of energy. The aim of this research is to examine the viability of growing different types of energy crops on metal contaminated land using chemical amendments. This study has involved laboratory phytotoxicity tests, pot experiments and a field demonstration trial. The site selected for this project is located near Fuyang in Zhejiang province, People’s Republic of China and is located adjacent to a former copper smelter. The four energy crops trialled at the field site are sweet sorghum, sugar cane, Chinese sumac and vetiver grass. Chinese sumac and sweet sorghum were also used in the pot experiments. The methodology has involved trialling a combination of chemical treatments to immobilise the available metals in the soil in order to alleviate toxicity to the crops, along with the selection of locally available crop species which are tolerant to the soil conditions. The chemical treatments trialled included the addition of three different clay minerals (attapulgite, bentonite and zeolite), phosphate rock and lime. The surface soils at the site are contaminated with metals (predominantly copper and zinc). All soils used in the laboratory experiments were from the site at Fuyang and were collected from the plot which has a high available copper concentration (2.63 mg/kg) (by sodium nitrate extraction) and a pH of approximately 6.1 in order to observe a ‘worst case’ scenario. The standard barley root elongation tests, based on International Organisation for Standardisation (ISO) 11269-1,1993, were undertaken to observe the short-term effects of various treatments of the Fuyang soil, with respect to alleviating phytotoxicity to barley, and with the aim to determine the most effective amendment(s) or their combination. The aim of the pot experiments was to study the long-term effects on the crops using the Fuyang soil combined with the most effective amendments determined following the root elongation tests. The objective of the field trial was to study the long-term effects on the energy crops at an actual site with metal contaminated soils and observe plant biomass and metal accumulation using selected amendments based on the laboratory experiments over one harvest period. Lime was the most effective amendment trialled at decreasing available copper and zinc concentrations and… Advisors/Committee Members: Jaksa, Mark Brian (advisor), Daniell, Trevor Maurice (advisor), School of Civil, Environmental and Mining Engineering (school).

Subjects/Keywords: energy crops; metals; phytotoxicity; contaminated land; amendments; immobilisation; sorghum; sugar cane

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Egan, S. (2012). Suitability of energy cropping on abandoned metal contaminated farmland. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/77087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Egan, Sally. “Suitability of energy cropping on abandoned metal contaminated farmland.” 2012. Thesis, University of Adelaide. Accessed March 06, 2021. http://hdl.handle.net/2440/77087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Egan, Sally. “Suitability of energy cropping on abandoned metal contaminated farmland.” 2012. Web. 06 Mar 2021.

Vancouver:

Egan S. Suitability of energy cropping on abandoned metal contaminated farmland. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2440/77087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Egan S. Suitability of energy cropping on abandoned metal contaminated farmland. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/77087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Bou Saab, Hamid. Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica.

Degree: Docteur es, Chimie-Biologie, 2011, Mulhouse

URL: http://www.theses.fr/2011MULH6411

►

L'un des avantages majeurs des réactions de bioconversion résulte du fait que le milieu réactionnel des biocatalyseurs est l'eau. Ce dit avantage constitue l'une des… (more)

▼

L'un des avantages majeurs des réactions de bioconversion résulte du fait que le milieu réactionnel des biocatalyseurs est l'eau. Ce dit avantage constitue l'une des principales limitations de ces réactions de bioconversion lorsqu'il s'agit de substances lipophiles non solubles dans l'eau connue les stérols. L'efficacité d'un procédé de bioconversion de substances lipophiles solides dépend essentiellement du contact et de 1'interaction entre le biocatalyseur et ce substrat lipophile. Les solutions proposées dans la littérature font appel à des solvants et des produits chimiques de natures souvent toxiques, inflammables et explosives. Ces solutions décrites font perdre à la bioconversion son caractère de biotechnologie blanche. Dans ce travail, nous avons montré qu'en plus de ses avantages connus, l'immobilisation passive de biocatalyseurs au sein d'un support poreux peut favoriser l'interaction cellule-substrat lipophile et augmenter le taux de bioconversion sans utiliser de solvants et de produits chimiques. La réaction modèle étudiée est le clivage de la chaine latérale des stérols par des mycobactéries en vue de l'obtention des androsténones précurseurs naturels des stéroïdes, molécules à forte valeur biologique ajoutée. Le support d'immobilisation le plus performant a été le fruit sec de Luffa cylindrica. Par rapport aux supports organiques classiques tels que les gels de polyacrylamide, les mousses de polyuréthane, la silicone et les plastiques, le fruit sec de Luffa cylindrica présente les avantages suivants : (i) c'est un produit naturel, (ii) biodégradable, (iii) peu onéreux, (iv) non toxique pour les microorganismes, (v) stable du point de vue mécanique et thermique, (vi) et réutilisable.

One of the major advantages of using biocatalysts in organic synthesis is that water constitutes the reaction medium. However, water becomes a serious problem when bioconversion deals with lipophilic compounds, in particular those poorly soluble in water such as sterols. Bioconversion of lipophilic compounds depends on the close contact between the hydrophobic substrate and the biocatalyst. Increasing this contact requires usually the use of huge amounts of chemical which are often toxic, flammable and explosive. In this work, we showed that passive cell immobilization in porous materials can increase the contact between microorganisms and lipophilic substrates without using chemicals. The side chain cleavage of sterols was studied as a model multistep microbial bioconversion of lipophilic compounds. This reaction allows the production of androstenones which are the natural precursors of steroids. Among the studied immobilization carriers, the dried fruit of Luffa cylindrica was the most efficient Compared to other organic support carrier such as alginate beads, polyurethane foams, silicones and plastics, the dried fruit of Luffa cylindrica is advantageous since it is natural, renewable, biodegradable, cheap, mechanically strong, free of toxicity and it doesn't need a chemical pretreatment.

Advisors/Committee Members: Neunlist, Serge (thesis director).

Subjects/Keywords: Bioconversion; Immobilisation; Stérols; Androstènedione; Androstadiènedione; Bioconversion; Immobilization; Sterols; Androstenedione; Androstadienedione

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bou Saab, H. (2011). Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica. (Doctoral Dissertation). Mulhouse. Retrieved from http://www.theses.fr/2011MULH6411

Chicago Manual of Style (16^th Edition):

Bou Saab, Hamid. “Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica.” 2011. Doctoral Dissertation, Mulhouse. Accessed March 06, 2021. http://www.theses.fr/2011MULH6411.

MLA Handbook (7^th Edition):

Bou Saab, Hamid. “Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica.” 2011. Web. 06 Mar 2021.

Vancouver:

Bou Saab H. Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica. [Internet] [Doctoral dissertation]. Mulhouse; 2011. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2011MULH6411.

Council of Science Editors:

Bou Saab H. Bioconversion éco-compatible de triterpénoïdes par des bactéries immobilisées sur Luffa cylindrica : Eco-friendly bioconversion of triterpenoids by bacteria immobilized on Luffa cylindrica. [Doctoral Dissertation]. Mulhouse; 2011. Available from: http://www.theses.fr/2011MULH6411

Delft University of Technology

20. De Lathouder, K.M. Development of a monolithic bioreactor: Design and application.

Degree: 2007, Delft University of Technology

URL: http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a

► Recently, biocatalysis has become an interesting alternative for conventional industrial processes. Enzyme reactions are highly selective and fast, and can be performed under ambient conditions.… (more)

Subjects/Keywords: monolith; biocatalysis; bioreactor; immobilisation; acm

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

De Lathouder, K. M. (2007). Development of a monolithic bioreactor: Design and application. (Doctoral Dissertation). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a

Chicago Manual of Style (16^th Edition):

De Lathouder, K M. “Development of a monolithic bioreactor: Design and application.” 2007. Doctoral Dissertation, Delft University of Technology. Accessed March 06, 2021. http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a.

MLA Handbook (7^th Edition):

De Lathouder, K M. “Development of a monolithic bioreactor: Design and application.” 2007. Web. 06 Mar 2021.

Vancouver:

De Lathouder KM. Development of a monolithic bioreactor: Design and application. [Internet] [Doctoral dissertation]. Delft University of Technology; 2007. [cited 2021 Mar 06]. Available from: http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a.

Council of Science Editors:

De Lathouder KM. Development of a monolithic bioreactor: Design and application. [Doctoral Dissertation]. Delft University of Technology; 2007. Available from: http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; urn:NBN:nl:ui:24-uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a ; http://resolver.tudelft.nl/uuid:a214b1ce-77e5-40db-a2b1-1995e24fd73a

University of Sydney

21. Cheng, Xinying. Shape Memory Polymers with Multifaceted Tunability and Bio-specificity .

Degree: 2017, University of Sydney

URL: http://hdl.handle.net/2123/17130

► Shape memory polymers (SMP) have been of great interest in biomedical applications due to their unique ability to recover a primary shape by external actuation.… (more)

Subjects/Keywords: shape memory polymer; polyurethane; plasma immersion ion implantation; biocompatibility; protein immobilisation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cheng, X. (2017). Shape Memory Polymers with Multifaceted Tunability and Bio-specificity . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheng, Xinying. “Shape Memory Polymers with Multifaceted Tunability and Bio-specificity .” 2017. Thesis, University of Sydney. Accessed March 06, 2021. http://hdl.handle.net/2123/17130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheng, Xinying. “Shape Memory Polymers with Multifaceted Tunability and Bio-specificity .” 2017. Web. 06 Mar 2021.

Vancouver:

Cheng X. Shape Memory Polymers with Multifaceted Tunability and Bio-specificity . [Internet] [Thesis]. University of Sydney; 2017. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2123/17130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheng X. Shape Memory Polymers with Multifaceted Tunability and Bio-specificity . [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

22. Garton, Fleur. The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved .

Degree: 2014, University of Sydney

URL: http://hdl.handle.net/2123/11849

► A common null polymorphism (R577X) in the ACTN3 gene results in complete absence of α-actinin-3 in ~16% of the world’s population. α-Actinin-3 deficiency is detrimental… (more)

Subjects/Keywords: alpha-actinin-3; alpha-actinin-2; denervation; immobilisation; rAAV

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garton, F. (2014). The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/11849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Garton, Fleur. “The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved .” 2014. Thesis, University of Sydney. Accessed March 06, 2021. http://hdl.handle.net/2123/11849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Garton, Fleur. “The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved .” 2014. Web. 06 Mar 2021.

Vancouver:

Garton F. The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved . [Internet] [Thesis]. University of Sydney; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/2123/11849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garton F. The role of a-actinin-3 in muscle adaptation to changing physical demands and the molecular mechanisms involved . [Thesis]. University of Sydney; 2014. Available from: http://hdl.handle.net/2123/11849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund

23. Khan, Sami. Molecular development of a thermostable β-glucosidase for modification of natural products.

Degree: 2012, University of Lund

URL: https://lup.lub.lu.se/record/2256960 ; https://portal.research.lu.se/ws/files/6256806/2256968.pdf

► The gene encoding a β-glucosidase originating from the extreme thermophile Thermotoga neopolitana has been cloned and expressed in Escherichia coli. The aim was to produce… (more)

▼ The gene encoding a β-glucosidase originating from the extreme thermophile Thermotoga neopolitana has been cloned and expressed in Escherichia coli. The aim was to produce a thermostable enzyme that could be used to remove sugar residues from glucosylated natural products classified as flavonoids by applying a method combining extraction in hot pressurized water with enzymatic hydrolysis. The β-glucosidase (termed TnBgl1A in this thesis) is a member of family 1 of glycoside hydrolase (GH1). The enzyme has an apparent unfolding temperature of 101.9 °C and a molecular weight of 52.6 kDa. The activity of TnBgl1A was first analysed using the model substrate para-nitrophenyl-β-D-glucopyranoside (pNPGlc), demonstrating that a single glucosyl residue (typical for β-glucosidases) is released by this enzyme. Hydrolysis of glucosylated forms of the natural product quercetin (the major flavonoid in yellow onion) was found to be dependent on the position of the glucosylation (see below). Expression in E. coli resulted in a relatively large fraction of insoluble target protein. To improve the folding of TnBgl1A during production, different strategies were then applied: I) the gene was constructed synthetically and codons were optimized to match codon usage in E.coli, II) the gene was cloned in frame with a signal peptide to translocate the protein to the periplasmic space, and III) the gene was co-expressed with genes encoding molecular chaperones. Among these strategies the co-expression of the gene with chaperones worked best, and the improved folding resulted in an increased fraction of soluble, active enzyme. The TnBgl1A was tested for the hydrolysis of quercetin-glucosides, which are antioxidants classified as flavonoids present in onion, and composed of a polyphenolic backbone glucosylated at two different positions (Q3, Q4´, and the diglucoside Q3,4´). The aglycone form of quercetin is a more potent antioxidant than the glucosylated forms. The activity of TnBgl1A for Q3 was lower compared to its activity on quercetin-4´-glucoside (Q4´). To improve hydrolysis of Q3, mutations were introduced in the enzyme, based on a structure model of TnBgl1A. The mutant N221S/P342L showed increased efficiency towards Q3 as well as for Q4´ compared to wild-type. This showed that the position and nature of this residue in the active site is important for substrate specificity and that by careful selection the specificity can be changed for different substrates. Therefore, the mutation studies were extended and the active site region was targeted for further mutagenesis. Among the changes introduced, the mutagenesis of the neighbouring residue, N220S, was also found to influence activity, and this variant had a higher specific activity for quercetin-glucosides. TnBgl1A and one of the best performance mutants (N221S/P342L) were immobilized on acrylic support to allow recycling of the enzyme in experiments coupling hot water extraction of quercetin-glucosides with enzymatic hydrolysis. The activity of the immobilised enzyme was analysed in…

Subjects/Keywords: Engineering and Technology; Thermotoga neapolitana; TnBgl1A; Mutagenesis; Flavonoid; Quercetin; Immobilisation; Chaperones

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Khan, S. (2012). Molecular development of a thermostable β-glucosidase for modification of natural products. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/2256960 ; https://portal.research.lu.se/ws/files/6256806/2256968.pdf

Chicago Manual of Style (16^th Edition):

Khan, Sami. “Molecular development of a thermostable β-glucosidase for modification of natural products.” 2012. Doctoral Dissertation, University of Lund. Accessed March 06, 2021. https://lup.lub.lu.se/record/2256960 ; https://portal.research.lu.se/ws/files/6256806/2256968.pdf.

MLA Handbook (7^th Edition):

Khan, Sami. “Molecular development of a thermostable β-glucosidase for modification of natural products.” 2012. Web. 06 Mar 2021.

Vancouver:

Khan S. Molecular development of a thermostable β-glucosidase for modification of natural products. [Internet] [Doctoral dissertation]. University of Lund; 2012. [cited 2021 Mar 06]. Available from: https://lup.lub.lu.se/record/2256960 ; https://portal.research.lu.se/ws/files/6256806/2256968.pdf.

Council of Science Editors:

Khan S. Molecular development of a thermostable β-glucosidase for modification of natural products. [Doctoral Dissertation]. University of Lund; 2012. Available from: https://lup.lub.lu.se/record/2256960 ; https://portal.research.lu.se/ws/files/6256806/2256968.pdf

24. Salwiński, Aleksander. Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe.

Degree: Docteur es, Chimie, 2014, Université d'Orléans

URL: http://www.theses.fr/2014ORLE2013

►

Ce manuscrit de thèse présente le développement de méthodes basées sur la spectrométrie de masse consacrées à la recherche d'inhibiteurs d'enzymes en milieux complexes, tels… (more)

▼

Ce manuscrit de thèse présente le développement de méthodes basées sur la spectrométrie de masse consacrées à la recherche d'inhibiteurs d'enzymes en milieux complexes, tels que les extraits de plantes. L’enzyme Tyrosinase a été utilisé comme principale cible biologique du fait de son implication dans les processus d’hyperpigmentation cutanée. De ce fait, la recherche d’inhibiteurs de cette enzyme, présente un grand intérêt pour l'industrie cosmétique. La première partie de ce manuscrit décrit la mise en place de la chromatographie d'affinité frontale (FAC), permettant d’obtenir le classement simultané des inhibiteurs présent dans un mélange complexe en fonction de leurs affinités avec la cible biologique. Deux capillaires hydrophiles de phase monolithiques ont été évalués afin de réduire au maximum les interactions non spécifiques indésirables entre les analytes et le support solide d’immobilisation. De plus, nous avons étudié la faisabilité de l’utilisation de phases à base de silice comme support solide d’immobilisation des enzymes dans le cadre de ces analyses par chromatographie d'affinité frontale. La seconde partie du manuscrit de thèse est consacrée au développement et à l’optimisation de l’approche nommée ENALDI-MS (Enzyme-coupled Nanoparticles-Assisted Laser Desorption/Ionisation Mass Spectrometry) permettant d’accéder à une gamme des faibles masses (m/z 500 Da). Elle est déclinée en une première approche dite par ‘extinction d’ions’ (Ion Fading, IF-ENALDI), basée sur l’identification directe de la liaison des inhibiteurs vis-à-vis de l’enzyme sans pré-traitement de l’échantillon végétal. Une seconde déclinaison de l’ENALDI-MS concerne une approche dite par ‘Ion Hunting’ (IH - ENALDI MS), basée sur une méthode de pré-concentration sélective des inhibiteurs présents dans l'échantillon.

This thesis report presents the development of mass spectrometry-based methods for searching for inhibitors of enzymes in complex mixtures, such as plant extracts. Tyrosinase enzyme was used as the main biological target for the reason of a significant importance of its inhibitors in the cosmetic industry as the skin whitening agents. The first part of this report describes Frontal Affinity Chromatography (FAC), an approach enabling simultaneous ranking the inhibitors within the complex mixture according to their affinities to the biological target. Two hydrophilic capillary-scale polymer-based bioaffinity stationary phases were evaluated in the context of the presence of undesirable nonspecific interactions between the analyte and the solid immobilisation support. In addition, we explored the usability of two types of silica-based particles as a solid support for enzyme immobilisation for FAC. The second part of the thesis manuscript is devoted to Enzyme-coupled Nanoparticle-Assisted Laser Desorption/Ionisation Mass Spectrometry (ENALDI MS) as a low-mass compatible extension of the Intensity ion Fading MALDI MS (IF-MALDI MS) method for high-throughput screening of the inhibitors in the complex mixtures. Two variations of…

Advisors/Committee Members: Maunit, Benoît (thesis director), Delépée, Raphaël (thesis director).

Subjects/Keywords: Tyrosinase; Chromatographie d'affinité frontale; Monolithes; Immobilisation d’enzymes; Désorption/ionisation laser assistée par surface; Tyrosinase; Frontal affinity chromatography; Monoliths; Enzyme immobilisation; Surface-assisted laser desorption/ionisation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salwiński, A. (2014). Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe. (Doctoral Dissertation). Université d'Orléans. Retrieved from http://www.theses.fr/2014ORLE2013

Chicago Manual of Style (16^th Edition):

Salwiński, Aleksander. “Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe.” 2014. Doctoral Dissertation, Université d'Orléans. Accessed March 06, 2021. http://www.theses.fr/2014ORLE2013.

MLA Handbook (7^th Edition):

Salwiński, Aleksander. “Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe.” 2014. Web. 06 Mar 2021.

Vancouver:

Salwiński A. Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe. [Internet] [Doctoral dissertation]. Université d'Orléans; 2014. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2014ORLE2013.

Council of Science Editors:

Salwiński A. Development of novel mass spectrometry-based approaches for searching for low-mass tyrosinase inhibitors in complex mixtures : Développement de nouvelles approches basées sur la spectrométrie de masse pour le criblage d’inhibiteurs de la tyrosinase en milieu complexe. [Doctoral Dissertation]. Université d'Orléans; 2014. Available from: http://www.theses.fr/2014ORLE2013

25. Krausova Rambure, Katerina. Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator.

Degree: Docteur es, Sciences et Techniques de l'Environnement, 2013, Université Paris-Est

URL: http://www.theses.fr/2013PEST1118

►

L'objectif général de cette thèse est de transformer les déchets ultimes et dangereux contenant des métaux lourds, en matières minérales chimiquement stables. L'augmentation de la… (more)

▼

L'objectif général de cette thèse est de transformer les déchets ultimes et dangereux contenant des métaux lourds, en matières minérales chimiquement stables. L'augmentation de la production des ordures ménagères (OM) est un problème qui concerne et préoccupe le monde entier. Parmi les différentes méthodes de traitement des déchets municipaux, l'incinération est une technologie qui peut fournir une solution efficace et respectueuse pour l'environnement. Le problème de ce traitement est la production de REFIOM. Les REFIOM peuvent contenir de grandes quantités de composés métalliques toxiques et est considéré comme un déchet dangereux ce qui oblige à les mettre en décharges classées. Trois types de matériaux pour l'immobilisation du plomb et du cadmium ont été étudiés : les vitrocéramiques, les céramiques frittées et les géopolymères. Nous sommes parvenus à synthétiser une base de vitrocéramique à partir de cendres incinérées purs et nous avons diminué sa volatilisation lors de sa production. Des résultats prometteurs ont été obtenus pour la vitrocéramique Ca-Mg-Si-O qui a incorporé durablement une quantité élevée de cadmium dans les structures cristallines et le plomb dans la structure amorphe. La phase cristalline est plus résistante à l'attaque acide en raison de son incorporation dans une matrice vitreuse qui génère une double protection. Ses travaux ouvrent la possibilité de créer cette matrice en ajoutant des oxydes commerciaux aux résidus d'incinération. Nous avons étudié la céramique frittée à base du système Ba-Mg-Ti-O. Nous avons obtenu les trois phases minérales présentées dans la céramique SYNROC (hollandite, pérovskite et rutile) où le cadmium substitue le magnésium tandis que le plomb a occupé le site appartenant au baryum. La céramique frittée est satisfaisante en termes d'immobilisation des métaux lourds avec de bonnes propriétés physiques. Pour produire des géopolymères résistants à partir de REFIOM, il est avantageux d'utiliser un rapport L/S = 1,2 et de les sécher à température ambiante. Il a été observé que le frittage influence le taux de réorganisation structurelle avec l'apparition de la phase sodalite ( Na4Si3Al3O12Cl), qui se compose de tunnels où les métaux lourds peuvent être incorporés. Le plomb et le cadmium restent largement en dessous de la limite des normes TCLP. Le traitement thermique à plus de 500°C augmente la densité de l'échantillon. Lorsque les REFIOM sont mélangés avec d'autres types de déchets tels que des MIOM ou du calcin, il est possible d'augmenter la résistance chimique et mécanique. Nous avons conclu que les trois matrices de stockages étudiées offrent de bonne perspective pour l'immobilisation du plomb et du cadmium

The overall objective of this thesis is to transform ultimate and hazardous waste containing heavy metals, into chemically stable mineral materials. The increasing municipal solid waste (MSW) generation is a problem ranging to global concern. Among various MSW treatment methods, incineration is a technology, which may provide an efficient and environmental…

Advisors/Committee Members: Gautron, Laurent (thesis director).

Subjects/Keywords: Déchets ménagers; Matrices de stockage; Immobilisation durable; Vitrocéramiques; Céramiques frittées; Géopolymères; Municipal Solid Waste; Mineral matrices; Immobilisation; Glass-ceramic; Sintered ceramic; Geopolymers

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krausova Rambure, K. (2013). Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator. (Doctoral Dissertation). Université Paris-Est. Retrieved from http://www.theses.fr/2013PEST1118

Chicago Manual of Style (16^th Edition):

Krausova Rambure, Katerina. “Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator.” 2013. Doctoral Dissertation, Université Paris-Est. Accessed March 06, 2021. http://www.theses.fr/2013PEST1118.

MLA Handbook (7^th Edition):

Krausova Rambure, Katerina. “Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator.” 2013. Web. 06 Mar 2021.

Vancouver:

Krausova Rambure K. Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator. [Internet] [Doctoral dissertation]. Université Paris-Est; 2013. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2013PEST1118.

Council of Science Editors:

Krausova Rambure K. Vers de nouvelles matrices minérales pour l’immobilisation et la valorisation des déchets ultimes de l’incinération des déchets ménagers : New mineral matrices for the immobilisation and valorisation of fly and bottom ashes from municipal solid Waste incinerator. [Doctoral Dissertation]. Université Paris-Est; 2013. Available from: http://www.theses.fr/2013PEST1118

University of Waikato

26. Rajan, Anila. Removal of flatulence causing sugars in soymilk .

Degree: 2009, University of Waikato

URL: http://hdl.handle.net/10289/3604

► The enzyme, alpha-galactosidase was extracted from peanuts using various buffers and solvents. The specific activity of soluble preparation of peanut ranged from 0.0083 to 0.0606… (more)

Subjects/Keywords: Alpha -galactosidase; immobilisation; raffinose; stachyose; soymilk

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rajan, A. (2009). Removal of flatulence causing sugars in soymilk . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/3604

Chicago Manual of Style (16^th Edition):

Rajan, Anila. “Removal of flatulence causing sugars in soymilk .” 2009. Masters Thesis, University of Waikato. Accessed March 06, 2021. http://hdl.handle.net/10289/3604.

MLA Handbook (7^th Edition):

Rajan, Anila. “Removal of flatulence causing sugars in soymilk .” 2009. Web. 06 Mar 2021.

Vancouver:

Rajan A. Removal of flatulence causing sugars in soymilk . [Internet] [Masters thesis]. University of Waikato; 2009. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10289/3604.

Council of Science Editors:

Rajan A. Removal of flatulence causing sugars in soymilk . [Masters Thesis]. University of Waikato; 2009. Available from: http://hdl.handle.net/10289/3604

27. Kengne-Momo, Rosine Pélagie. Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering.

Degree: Docteur es, Physique, 2011, Le Mans; Université de Yaoundé I

URL: http://www.theses.fr/2011LEMA1005

►

L’objectif de cette thèse est de synthétiser de nouvelles surfaces spécifiques nécessaires à l’immobilisation des biomolécules ; visant à développer à terme un biocapteur pour… (more)

▼

L’objectif de cette thèse est de synthétiser de nouvelles surfaces spécifiques nécessaires à l’immobilisation des biomolécules ; visant à développer à terme un biocapteur pour la détection de pathogènes en industrie agroalimentaire. Cette nouvelle procédure de fonctionnalisation de surface consiste d’une part à greffer des molécules organiques sur un substrat métallique à partir d’une réaction électrochimique et d’autre part de synthétiser un monomère photopolymérisable sur tout type de surface. Ces surfaces sont enfin utilisées pour immobiliser les biomolécules. Ce procédé ainsi développé permet d’éliminer les multiples étapes, l’utilisation excessive de réactifs observés dans les protocoles classiques de fonctionnalisation de surface pour la capture de microorganismes. Deux stratégies de fonctionnalisation ont été investiguées : la polymérisation sur une plaque de platine et le dépôt de monocouche sur une surface d’or. La fonctionnalisation de surfaces ainsi que l’immobilisation de biomolécules ont été caractérisées par la spectroscopie Raman, la microbalance à cristal de quartz, la microscopie à force atomique (AFM) pour le premier et en plus la microscopie à fluorescence pour le second. Les résultats de la fonctionnalisation de surfaces par dépôt de polymère ont montré, une déstabilisation du polymère en présence de l’eau. Afin d’optimiser la synthèse, nous avons travaillé en milieu inerte, sous alumine activée. De plus, on note une large couverture de la zone spectrale des biomolécules par les signaux du polymère ; Pour le dépôt de monocouche, l’on a obtenu une surface très réactive, homogène. La diffusion Raman est la principale technique de caractérisation utilisée. Elle présente l'avantage d'être une méthode de caractérisation physico-chimique non destructive et non invasive. Longtemps délaissée dans les sciences du vivant, cette méthode apparaît maintenant particulièrement prometteuse grâce à un développement récent de spectromètres intégrés performants. La diffusion Raman sur la monocouche déposée montre une intensité accrue des signaux par l’utilisation de la surface d’or et un spectre plus dégagé conduisant à l’identification aisée des biomolécules après fixation. Elle permet non seulement d’identifier les bandes de vibrations de chaque groupement mais aussi la conformation des structures. Les résultats d’immobilisation ont montré que l’accroche des biomolécules sur les surfaces fonctionnalisées était spécifique. La fonctionnalisation de surface d’or par dépôt de monocouche constitue finalement une technique très rapide à mettre en œuvre, peu coûteuse permettant d’ancrer efficacement les biomolécules et peut être utilisée pour diverses applications. La synthèse du monomère photopolymérisable a été abordée et est en cours d’investigation.

In food processing industry, detecting bacteria or viruses is crucial. Nowadays, it can be achieved with microbiological tests but, it requires several days. The objective of the project was to synthesize new specific surfaces capable of biomolecules immobilization in…

Advisors/Committee Members: Daniel, Philippe (thesis director), Dongo, Etienne (thesis director).

Subjects/Keywords: Microbalance à cristal de quartz; Immobilisation de biomolécules; Biomolecules immobilization; Quartz Crystal Microbalance

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kengne-Momo, R. P. (2011). Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering. (Doctoral Dissertation). Le Mans; Université de Yaoundé I. Retrieved from http://www.theses.fr/2011LEMA1005

Chicago Manual of Style (16^th Edition):

Kengne-Momo, Rosine Pélagie. “Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering.” 2011. Doctoral Dissertation, Le Mans; Université de Yaoundé I. Accessed March 06, 2021. http://www.theses.fr/2011LEMA1005.

MLA Handbook (7^th Edition):

Kengne-Momo, Rosine Pélagie. “Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering.” 2011. Web. 06 Mar 2021.

Vancouver:

Kengne-Momo RP. Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering. [Internet] [Doctoral dissertation]. Le Mans; Université de Yaoundé I; 2011. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2011LEMA1005.

Council of Science Editors:

Kengne-Momo RP. Mise en oeuvre des surfaces spécifiques en vue de la détection de bactéries pathogènes par diffusion Raman : Elaboration of functionalized surfaces in the aim of pathogenic detection by Raman scattering. [Doctoral Dissertation]. Le Mans; Université de Yaoundé I; 2011. Available from: http://www.theses.fr/2011LEMA1005

University of Manchester

28. Kuippers, Gina. The Biodegradation of Isosaccharinic Acid.

Degree: 2017, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312420

► The nuclear waste inventory of the UK comprises large quantities of intermediate level wastes (ILW), which will be immobilised by encapsulation within a cementitious grout… (more)

▼ The nuclear waste inventory of the UK comprises large quantities of intermediate level wastes (ILW), which will be immobilised by encapsulation within a cementitious grout in stainless steel containers, followed by disposal in a deep engineered geological disposal facility (GDF) within a suitable geological formation. These wastes contain, in addition to radioactive elements, a heterogeneous mix of organic materials, including plastics, cellulose and rubber. Cellulosic items, such as cloth, tissue, filters, paper and wood, are considered particularly problematic, because they are known to be susceptible to degradation under alkaline conditions, forming small chain organic acids with the ability to complex metals and radionuclides. It is predicted that under alkaline conditions isosaccharinic acid (ISA) will form particularly strong complexes with Ni(II), Am(III), Eu(III), Np(IV), Th(IV), and U(IV). As a result, the presence of ISA could affect the migration behaviour of these elements, by increasing their solubility and reducing sorption, thus enhancing their mobility into the near and far field surrounding a GDF. During site operation and then after closure of a GDF, microbial communities have the potential to colonise the steep biogeochemical gradients, running from highly alkaline in the GDF â€œnear fieldâ€ to circumneutral pH conditions in the surrounding geosphere. Within these steep pH gradients microbial processes can control the fate of organic compounds, such as ISA, and have therefore been considered as an effective self-attenuating mechanism to remove ISA from the groundwater. This thesis aims to deliver a greater understanding of the microbial processes that can potentially use ISA as a carbon source and electron donor, removing it from solution, and thus having a positive impact on radionuclide mobility under GDF-relevant conditions. A microbial enrichment approach was chosen that approaches GDF-relevant conditions to explore the biodegradation of ISA. Cross-disciplinary analyses of water chemistry (pH, Eh, photospectroscopy, IC, ICP), mineralogy (ESEM, XRD, TEM, XAS) and microbiology (light microscopy, next generation sequencing) have demonstrated the ability of bacteria to degrade ISA over a wide range of biogeochemical conditions. Furthermore, key radionuclides (and their non-active analogues), including Ni(II) and U(VI), were precipitated from the groundwater system during ISA biodegradation. Moreover, in the case of uranium, microbial metabolism led to the reduction of U(VI) to U(IV), which is also less soluble. This study highlights the potential for microbial activity to help remove chelating agents from groundwaters surrounding an ILW GDF, and suggests that safety cases that do not include microbial processes may be overly conservative, over-estimating the impact of ISA on radionuclide transport. Advisors/Committee Members: BRYAN, NICHOLAS ND, Lloyd, Jonathan, Bryan, Nicholas.

Subjects/Keywords: biodegradation; isosaccharinic acid; uranium; nuclear waste; geodisposal; bio-barrier; radionuclide immobilisation; ISA; microbial degradation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuippers, G. (2017). The Biodegradation of Isosaccharinic Acid. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312420

Chicago Manual of Style (16^th Edition):

Kuippers, Gina. “The Biodegradation of Isosaccharinic Acid.” 2017. Doctoral Dissertation, University of Manchester. Accessed March 06, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312420.

MLA Handbook (7^th Edition):

Kuippers, Gina. “The Biodegradation of Isosaccharinic Acid.” 2017. Web. 06 Mar 2021.

Vancouver:

Kuippers G. The Biodegradation of Isosaccharinic Acid. [Internet] [Doctoral dissertation]. University of Manchester; 2017. [cited 2021 Mar 06]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312420.

Council of Science Editors:

Kuippers G. The Biodegradation of Isosaccharinic Acid. [Doctoral Dissertation]. University of Manchester; 2017. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:312420

INP Toulouse

29. Monteiro Centeno da Costa, Filipe. Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.

Degree: Docteur es, Génie des procédés et environnement, 2011, INP Toulouse

URL: http://www.theses.fr/2011INPT0061

►

L'étude et le développement des procédés de fabrication de levures immobilisées en vue de la réalisation de fermentations de vins a débuté au milieu des… (more)

▼

L'étude et le développement des procédés de fabrication de levures immobilisées en vue de la réalisation de fermentations de vins a débuté au milieu des années 80. Malgré les bénéfices potentiels que cette technologie pouvait apporter pour le secteur œnologique, peu de procédés d'immobilisation ont réussi à dépasser l'échelle laboratoire ou pilote et ceux qui sont arrivés à l'échelle industrielle n'ont pas eu le succès désiré pour des questions d'ordre technique ou économique. Le premier objectif de ce travail concerne la mise au point du procédé industriel en insistant sur les aspects les plus sensibles, et qui comme tels ont exigé des études complémentaires. Le deuxième objectif de ce travail vise à caractériser du point de vue cinétique et lorsque possible sensoriel, les fermentations avec les levures immobilisées pour la production de vins effervescents et pour la désacidification biologique de moûts. Le troisième et dernier objectif de ce travail consiste à évaluer l'utilisation de levures immobilisées pour la réalisation de la fermentation alcoolique en continu de moût. Pour cela on a fait appel à des fermenteurs continus à lit fixe et à lit fluidisé.

The study and development of yeast immobilization processes for wine fermentations started in the mid 80’s. Even though this technology could be of great benefit for the oenological sector very few process left the laboratory or pilot scale and those which arrived to industrial scale didn’t have the ambitioned success due to technical or economical constraints. The first goal of this work was to develop an industrial process for yeast immobilisation with emphasis on the most sensitive aspects which required further studies. The second objective of this work was to characterise the fermentation kinetics of immobilised yeasts cells during the production sparkling wines and during the deacidification of grape must. Whenever possible the wines produced were also characterised from a sensorial point of view. The third and last goal was to evaluate the use of immobilised yeast cells for continuous fermentation of grape must. For that we have used continuous fixed bed and fluidized bed fermenters.

Advisors/Committee Members: Strehaiano, Pierre (thesis director), Alliet, Marion (thesis director).

Subjects/Keywords: Immobilisation; Levures immobilisées fermentation; Alginate; Vin; Immobilization; Immobilized yeast fermentation; Alginate; Wine

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Monteiro Centeno da Costa, F. (2011). Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. (Doctoral Dissertation). INP Toulouse. Retrieved from http://www.theses.fr/2011INPT0061

Chicago Manual of Style (16^th Edition):

Monteiro Centeno da Costa, Filipe. “Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.” 2011. Doctoral Dissertation, INP Toulouse. Accessed March 06, 2021. http://www.theses.fr/2011INPT0061.

MLA Handbook (7^th Edition):

Monteiro Centeno da Costa, Filipe. “Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations.” 2011. Web. 06 Mar 2021.

Vancouver:

Monteiro Centeno da Costa F. Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. [Internet] [Doctoral dissertation]. INP Toulouse; 2011. [cited 2021 Mar 06]. Available from: http://www.theses.fr/2011INPT0061.

Council of Science Editors:

Monteiro Centeno da Costa F. Procédé d’immobilisation de levures pour applications oenologiques. Etudes des paramètres du procédé. Validations experimentales : Yeast immobilisation process for oenological applications. Process parameters. Experimental validations. [Doctoral Dissertation]. INP Toulouse; 2011. Available from: http://www.theses.fr/2011INPT0061

30. Barr, Katie. Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting .

Degree: 2014, AUT University

URL: http://hdl.handle.net/10292/6938

► The ability to immobilise biomolecules on solid surfaces whilst retaining their function is the foundation of microarrays, biosensors and other miniaturised assays that provide convenient… (more)

▼ The ability to immobilise biomolecules on solid surfaces whilst retaining their function is the foundation of microarrays, biosensors and other miniaturised assays that provide convenient ways of analysing binding reactions of molecules with their ligands. The stability, sensitivity, cost and ease of use are important considerations when designing these systems, with the immobilisation of the biomolecule to a surface being the key to success. Miniaturised assays can allow multiplex analysis of various samples from protein interactions, carbohydrate recognition events, DNA hybridisation and cell immobilisation. They can enable fast, high-throughput and efficient testing for diagnostics, environmental testing and research purposes, as well as enabling production of whole-cell arrays to tissue engineering. The drive to miniaturisation and integration has advanced the need for effective and stable attachment of biomolecules to solid surfaces which allow high probe density and high signal to noise ratio whilst maintaining the activity of the molecule. KODE™ technology is a surface engineering technology that enables modification of a cell or virion surface without causing harm. This is achieved with Function-Spacer-Lipid (FSL) constructs that embed into the membrane. They contain a functional head group that conveys a desirable attribute to the cell or virion, which include carbohydrate or peptide antigens, fluorescent markers, whole proteins or useful molecules for attachment, e.g. biotin. This research set out to determine if FSL constructs could modify non-biological surfaces as well as biological ones. The aims of this research were to establish whether FSL constructs could be attached to non-biological surfaces, and if so what the mechanisms of attachment and the limitations might be and what methods could be used to deliver the molecules to the surface. Inkjet printing was investigated as the primary delivery method, as it is becoming a popular approach to apply biological molecules to surfaces. It is a non-contact, precise, flexible and fast technique which lends itself well to this field where precise patterning, sample and waste volume and cost are important factors. The water soluble advantages of FSL constructs make their use as a “bioink” attractive and this method proved to be successful at applying FSLs to surfaces. FSL constructs were successfully applied to various surfaces including papers, polymers, metals, modified cellulose materials and natural fibres. All surfaces were able to attach the FSLs. The stability of the attachment was shown to be unchanged after 8 months at room temperature. Some detergents and solvents were able to remove the FSLs from the surface, indicating particular limitations of the attachment. Variations of the FSL structure gave insights into the attachment mechanisms which indicated that the amphiphilic nature of these constructs is important, with hydrophobic forces causing adsorption of these constructs onto solid surfaces. Investigations into… Advisors/Committee Members: Henry, Steve (advisor), Diegel, Olaf (advisor).

Subjects/Keywords: KODE technology; Biomolecule immobilisation; Bioprinting

…important considerations when designing these systems, with the immobilisation of the biomolecule… …and cell immobilisation. They can enable fast, high-throughput and efficient testing for… …immunoassays. FSL constructs appear to allow orientated immobilisation of biomolecules, spaced away… …providing a fast, simple and easily controlled approach for biomolecule immobilisation. ix… …microscope TLC Thin layer chromatography x Chapter 1 Introduction Immobilisation of biological…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barr, K. (2014). Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting . (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/6938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Barr, Katie. “Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting .” 2014. Thesis, AUT University. Accessed March 06, 2021. http://hdl.handle.net/10292/6938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Barr, Katie. “Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting .” 2014. Web. 06 Mar 2021.

Vancouver:

Barr K. Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting . [Internet] [Thesis]. AUT University; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/10292/6938.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barr K. Bio-modification of non-biological surfaces with function-spacer-lipid constructs by methods including bioprinting . [Thesis]. AUT University; 2014. Available from: http://hdl.handle.net/10292/6938

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations