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NSYSU
1.
Chen, Pei-hsuan.
Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4.
Degree: Master, Biological Sciences, 2008, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902108-125407 
► Tumor necrosis factor-alpha (TNF-α) is a potent proinflammatory cytokine, inducing the acute-phase response that leads to physiological changes that serve to eliminate the infecting organisms.…
(more)
▼ Tumor necrosis factor-alpha (TNF-α) is a potent proinflammatory cytokine, inducing the acute-phase response that leads to physiological changes that serve to eliminate the infecting organisms. Toll-like receptors (TLRs) comprise a family of pattern-recognition receptors that detect conserved molecular products of microorganisms. To evaluate the role of TNF-α and TLRs in Pseudomonas aeruginosa infection-induced mortality in thermal injured mice, the wild type (WT), Tnfrsf1a-/-, and TLR4-/- mice were injected the P. aeruginosa in the back at 8 hr after 30% total body surface area burn. At 24 hr after burn, lung tissues of mice were harvested for assays of wet/dry ratio; microvascular dysfunction, myeloperoxidase (MPO) activity, NF-κB DNA binding activity; and expression of IL-1β,
iNOS, p-JNK, and TLR4. Injection of P. aeruginosa after burn induced a survival rate in Tnfrsf1a-/- 60% TBSA burn=100%, WT 60% TBSA burn=10%, WT 30% TBSA burn +P.A = 60%, Tnfrsf1a-/- 30% TBSA burn+p.A. = 30%. respectively. The high mortality in Tnfrsf1a-/- mice is related to a significant increase of pulmonary microvascular dysfunction; neutrophil infiltration, bacterial counts of blood and lung; and expression of TLR4, IL-1β, and
iNOS as compared with WT mice. On the contrary, significant increase of NF-κB DNA binding activity of lung was observed only in WT mice. When
iNOS inhibitor SMT was given immediately after burn to WT and Tnfrsf1a-/- mice, the P. aeruginosa induced increases of pulmonary edema, pulmonary permeability, and lung bacterial counts were decreased significantly in Tnfrsf1a-/- mice, suggesting that TNF-α decreases P. aeruginosa-induced mortality in burned mice is through a negative regulation of TLR4 and
iNOS.
Advisors/Committee Members: Lee-wei Cheng (committee member), Ming-hong Tai (chair), Ching-mei Hsu (committee member), Li-chung Hsu (chair).
Subjects/Keywords: iNOS; TLR4
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APA (6th Edition):
Chen, P. (2008). Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902108-125407
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Pei-hsuan. “Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4.” 2008. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902108-125407.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Pei-hsuan. “Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4.” 2008. Web. 18 Jan 2021.
Vancouver:
Chen P. Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4. [Internet] [Thesis]. NSYSU; 2008. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902108-125407.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen P. Lack of TNF-α Receptor 1 Decreases Pseudomonas aeruginosa-induced Mortality in Burned Mice through Negative Regulation of Toll-like Receptor 4. [Thesis]. NSYSU; 2008. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0902108-125407
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Tampereen ammattikorkeakoulu
2.
Oksanen, Anne.
Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi.
Degree: 2013, Tampereen ammattikorkeakoulu
URL: http://www.theseus.fi/handle/10024/68947
► Makrofagit ovat veressä kiertävistä monosyyteistä erilaistuneita tulehdussoluja. Ne toimivat immuunipuolustuksessa fagosyytteina sekä antigeenejä esittelevinä soluina. Makrofagit tuottavat monia sytokiineja ja muita tulehduksen välittäjäaineita, ja ovat…
(more)
▼ Makrofagit ovat veressä kiertävistä monosyyteistä erilaistuneita tulehdussoluja. Ne toimivat immuunipuolustuksessa fagosyytteina sekä antigeenejä esittelevinä soluina. Makrofagit tuottavat monia sytokiineja ja muita tulehduksen välittäjäaineita, ja ovat osallisina tulehdusreaktion eri vaiheissa. Eri sytokiinit ohjaavat makrofagien aktivoitumista joko klassisen tai vaihtoehtoisen aktivaation suuntaan. Klassisesti aktivoituneet makrofagit toimivat ensisijaisesti tulehdusreaktion alkuvaiheessa, kun taas vaihtoehtoisesti aktivoituneita makrofageja tavataan pääasiassa paranemassa olevassa kudoksessa sekä kroonisessa tulehduksessa.
Immunohistokemia on värjäysmenetelmä, joka perustuu antigeenin ja sille spesifisen vasta-aineen väliseen vuorovaikutukseen. Menetelmästä riippuen joko primaarivasta-aine tai sekundaarivasta-aine on leimattu tavallisimmin entsyymileimalla, joka reagoidessaan substraatin kanssa muodostaa näkyvän värin. Opinnäytetyössä värjäyksissä käytettiin LabVision Autostainer –värjäysautomaattia.
Opinnäytetyössä kehitettiin ja optimoitiin värjäysmenetelmä neljälle eri vasta-aineelle. Vasta-aine CD68 tunnisti yleisesti kudoksen makrofageja, vasta-aineet CD163 ja CD206 vaihtoehtoisesti aktivoituneita makrofageja ja vasta-aine iNOS klassisesti aktivoituneita makrofageja. Kehitetyn värjäysmenetelmän todettiin olevan toimiva ja soveltuva käyttötarkoitukseensa. Menetelmää voidaan jatkossa käyttää erilaisten solu- ja kudosnäytteiden tutkimiseen ja kehittää edelleen kaksois- ja fluoresenssivärjäyksiin soveltuvaksi.
Macrophages are inflammatory cells which differentiate from circulating monocytes to macrophages following their migration into tissues. In the immune system macrophages function as phagocytes and antigen-presenting cells and they produce various cytokines and other mediators which direct and regulate the inflammatory response. Depending on the inflammatory and tissue environment, macrophages display different phenotypes with distinct functional properties. Cytokines produced by T lymphocytes guide the polarization of macrophages towards either classical (M1) or alternative (M2) activation. Classically activated macrophages are important effector cells in acute inflammation, while alternatively activated macrophages are found in recovering tissue and in chronic inflammation.
Immunohistochemistry is a staining technique based on specific antigen-antibody interaction. Binding of the antibody to its antigen is detected either by direct labeling of the antibody, or by the use of a secondary labeling method. Most widely used labels in immunohistochemistry are enzymes. When the labeling enzyme reacts with a proper substrate, it forms a visible precipitate. In the present study, immunohistochemical staining was carried out by using a LabVision Autostainer device.
In the present thesis an immunohistochemical staining technique was developed and optimized to detect M1 and M2 macrophage phenotypes by using four different antibodies. CD68 antibody was used as a common macrophage antibody to detect all…
Advisors/Committee Members: Tampereen ammattikorkeakoulu.
Subjects/Keywords: CD68; CD163; CD206; iNOS
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APA (6th Edition):
Oksanen, A. (2013). Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi. (Thesis). Tampereen ammattikorkeakoulu. Retrieved from http://www.theseus.fi/handle/10024/68947
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Oksanen, Anne. “Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi.” 2013. Thesis, Tampereen ammattikorkeakoulu. Accessed January 18, 2021.
http://www.theseus.fi/handle/10024/68947.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Oksanen, Anne. “Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi.” 2013. Web. 18 Jan 2021.
Vancouver:
Oksanen A. Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi. [Internet] [Thesis]. Tampereen ammattikorkeakoulu; 2013. [cited 2021 Jan 18].
Available from: http://www.theseus.fi/handle/10024/68947.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Oksanen A. Makrofagien polarisaation immunohistokemiallinen osoittaminen soluviljely- ja kudosnäytteistä : Menetelmän kehitys ja optimointi. [Thesis]. Tampereen ammattikorkeakoulu; 2013. Available from: http://www.theseus.fi/handle/10024/68947
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Duarte, Andressa.
Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais.
Degree: Mestrado, Imunologia Básica e Aplicada, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-21102013-112320/
;
► A imunidade inata é responsável pela resposta inicial aos microrganismos, uma vez que impede, controla ou elimina a infecção. Esse sistema consiste em barreiras epiteliais,…
(more)
▼ A imunidade inata é responsável pela resposta inicial aos microrganismos, uma vez que impede, controla ou elimina a infecção. Esse sistema consiste em barreiras epiteliais, proteínas plasmáticas e células circulantes e teciduais. Dentre esses componentes, os macrófagos possuem grande importância, sendo capazes de controlar e eliminar agentes patogênicos através da fagocitose e produção de espécies reativas de oxigênio e nitrogênio. A ativação de PRRs por constituintes oriundos dos patógenos em macrófagos desencadeia eventos da resposta imune inata, ativados por diversas vias de sinalização intracelular. A via das PI3Ks é conhecida por regular várias funções nas células, como a regulação do ciclo celular, migração e produção de espécies reativas de oxigênio e nitrogênio. O NO é um mediador central na imunidade inata que, após estímulos inflamatórios, é produzido em altas quantidades através da iNOS. Macrófagos deficientes em PI3K produzem menos NO e apresentam prejudicado controle da infecção quando infectados por T. cruzi. O objetivo do presente trabalho foi investigar o papel da via PI3K na produção de NO por macrófagos peritoneais estimulados com LPS. Os macrófagos empregados no estudo, WT e PI3K-/-, possuem o mesmo fenótipo. Observamos que macrófagos PI3K-/- possuem uma menor produção de NO e expressam menos iNOS. A reduzida expressão de iNOS, após estímulo com LPS, é também observada quando macrófagos WT são tratados com inibidores seletivos da PI3K e AKT. Além disso, demonstramos que, concomitantemente à menor expressão da iNOS, ocorre deficiência na fosforilação da AKT e diminuição da ativação do fator de transcrição NF-kB, sugerindo que a PI3K participa da ativação do NF-kB. Foi observado ainda que o tratamento com PTX também diminui a expressão da iNOS. No entanto, macrófagos PAFR-/- expostos ao LPS presentam maior expressão da iNOS, enquanto os macrófagos CCR2-/- apresentam menor expressão dessa enzima nessas condições. Para investigar a implicação da via PI3K in vivo foi administrado LPS i.v., como modelo de choque endotoxemico, no qual observamos maior sobrevida em animais PI3K-/- comparado aos animais WT e menores níveis de nitrito no soro. Nossos dados sugerem que a enzima PI3K é crítica para expressão de iNOS e produção de NO pelos macrófagos, possivelmente através da ativação do receptor CCR2, estando envolvida na fisiopatologia do choque induzido por LPS.
Innate immunity is the initial response to microorganisms, since it prevents, controls and eliminates infection. This system consists in epithelial barriers, plasma proteins and circulating and tissue cells. Among these components, macrophages have great importance, being capable of control and eliminate pathogen agents through phagocytosis and production of reactive oxygen and nitrogen species. Activation of PRRs by pathogens constituents in macrophages triggers events of the innate immune response, activated by various intracellular signaling pathways. PI3Ks pathway is known to regulate several functions in the cell, such as regulation of the…
Advisors/Committee Members: Cunha, Thiago Mattar.
Subjects/Keywords: iNOS; iNOS; NF-kB; NF-kB; NO; NO; PI3K; PI3K
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APA (6th Edition):
Duarte, A. (2013). Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17147/tde-21102013-112320/ ;
Chicago Manual of Style (16th Edition):
Duarte, Andressa. “Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais.” 2013. Masters Thesis, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/17/17147/tde-21102013-112320/ ;.
MLA Handbook (7th Edition):
Duarte, Andressa. “Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais.” 2013. Web. 18 Jan 2021.
Vancouver:
Duarte A. Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-21102013-112320/ ;.
Council of Science Editors:
Duarte A. Participação da via PI3K/AKT na produção de óxido nítrico por macrófagos peritoneais. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/17/17147/tde-21102013-112320/ ;
NSYSU
4.
Lin, Chi-Jui.
The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.
Degree: Master, Marine Biotechnology and Resources, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
► New bioactive compounds in microalgae increase their commercial values for healthy food. Nannochloropsis oculata is mostly used for juvenile feed, while application for health is…
(more)
▼ New bioactive compounds in microalgae increase their commercial values for healthy food. Nannochloropsis oculata is mostly used for juvenile feed, while application for health is unavailable so this research isolated the compositions of the anti-inflammatory ingredients from N. oculata. The results showed growth of N. oculata yielded well in 110 rpm, 25 °C, 15000 lux, and 2.8% salinity; additional nutrients and CaCO3 slightly increased production. The analysis of N. oculata anti-inflammatory ingredients was based on the inhibition ratios of
iNOS and COX-2 protein expression in RAW 264.7 under LPS induction. TLC separation showed that highly polar molecules inhibited
iNOS and COX-2 expressions. AWP2-11-1 and AWP2-11-3 compounds were from acetone partition. In RP-C18 separation of the extracts, A3-19-21 compound inhibited
iNOS and COX-2 expressions by 99% and 95%, respectively. HP2-69-70 compound from n-hexane partition inhibited
iNOS expression by 64%. The resulting compounds were identified for their structures via 1H-NMR, 13C-NMR, 2 D NMR, and mass spectrometries.
Advisors/Committee Members: Ping-Jyun Sung (committee member), Zhi-hong, Wen (chair), Chan-Shing Lin (committee member), Ming-Wei Lu (chair), Min-Ying Wang (chair).
Subjects/Keywords: Nannochloropsis oculata; anti-inflammatory; iNOS; COX-2
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APA ·
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APA (6th Edition):
Lin, C. (2014). The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Chi-Jui. “The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.” 2014. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Chi-Jui. “The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.” 2014. Web. 18 Jan 2021.
Vancouver:
Lin C. The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin C. The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
5.
Lee, Hsin-Pai.
The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats.
Degree: PhD, Marine Biotechnology and Resources, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-104856
► An acute gout attack manifests in the joint as dramatic inflammation. To date, the clinical use of medicinal agents has typically led to undesirable side…
(more)
▼ An acute gout attack manifests in the joint as dramatic inflammation. To date, the clinical use of medicinal agents has typically led to undesirable side effects. Numerous efforts have failed to create an effective and safe agent for the treatment of gout. Lemnalolâan extract from Formosan soft coralâhas documented anti-inflammatory and anti-nociceptive properties. We attempt to examine the therapeutic effects of lemnalol on intra-articular monosodium urate (MSU)-induced gouty arthritis in rats and found that treatment with lemnalol (intramuscular [im]) significantly attenuated MUS-induced mechanical allodynia, paw edema, and knee swelling. Histomorphometric and immunohistochemistry analysis revealed that MSU-induced inflammatory cell infiltration as well as the elevated expression of c-Fos and pro-inflammatory proteins (inducible nitric oxide synthase and cyclooxygenase-2) observed in synovial tissue were significantly inhibited by treatment with lemnalol. Moreover, we also investigated the effects of lemnalol, on mast cells function and osteoclast activities in gout arthritis. We found that both infiltration and degranulation of mast cells increased at 24 h after MSU injection in the ankle joint. Immunohistochemical analysis showed that MSU induced upregulation of TGF-β1 and MMP-9 and the osteoclast markers cathepsin K and TRAP in ankle tissues. We conclude that lemnalol treatment may be beneficial for improving gouty arthritis symptoms and signs, and attenuation the inflammatory reaction including neutrophils, mast cells infiltration and mast cell degranulation and for suppressing osteoclast activation in gouty arthritis. Lemnalol may be a promising candidate for the development of a new treatment for gout and other acute inflammatory arthritis diseases.
Advisors/Committee Members: Ming-Hong Tai (chair), Chang-Yi, Wu (chair), Hui-Min Wang (chair), Zhi-hong, Wen (committee member), Yen-Hsuan Jean (chair).
Subjects/Keywords: osteoclast; COX-2; iNOS; inflammation; lemnalol; gout
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lee, H. (2014). The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-104856
Chicago Manual of Style (16th Edition):
Lee, Hsin-Pai. “The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats.” 2014. Doctoral Dissertation, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-104856.
MLA Handbook (7th Edition):
Lee, Hsin-Pai. “The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats.” 2014. Web. 18 Jan 2021.
Vancouver:
Lee H. The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats. [Internet] [Doctoral dissertation]. NSYSU; 2014. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-104856.
Council of Science Editors:
Lee H. The anti-inflammatory effects of lemnalol on monosodium urate crystal-induced arthritis in rats. [Doctoral Dissertation]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-104856
Univerzitet u Beogradu
6.
Zafirović, Sonja S., 1983-.
Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova.
Degree: Biološki fakultet, 2018, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular Endocrinology
Estradiol ostvaruje pozitivan efekat na kardiovaskularni sistem (KVS), sprečavanjem nastanka ateroskleroze i endotelne i/ili vaskularne disfunkcije,…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular Endocrinology
Estradiol ostvaruje pozitivan efekat na
kardiovaskularni sistem (KVS), sprečavanjem nastanka ateroskleroze
i endotelne i/ili vaskularne disfunkcije, a deluje i direktno na
srce smanjujući njegovu hipertrofiju. Estradiol ostvaruje pozitivne
efekte u KVS regulacijom azot-monoksid (NO)-sintaza (NOS),
endotelne (eNOS) i inducibilne (iNOS) forme, koje su odgovorne za
sintezu NO, aktivacijom sloţene mreţe unutarćelijskih signalnih
puteva, u koje su uključeni signalni molekuli supstrat receptora za
insulin 1 (IRS-1), fosfatidil-inozitol-3-kinaza (PI3K) i protein
kinaza B (Akt). Estradiol utiče na signalnu transdukciju
angiotenzina II (Ang II), delujući na ekspresiju receptora za Ang
II tipa 1 (AT1R) i tipa 2 (AT2R), kao i na nivo RhoA proteina.
Estradiol utiče i na transport energetskih supstrata regulišući
translokazu masnih kiselina (CD36) i transportere za glukozu tipa 1
(GLUT1) i tipa 4 (GLUT4). Kontrolni i gojazni muţjaci pacova soja
Wistar tretirani su estradiolom (40μg/kg) ili 1% etanolom 24 sata
pre ţrtvovanja. Spektrofotometrijskim metodama određivane su
koncentracije NO u plazmi i koncentracije L-Arginina (L-Arg), NO i
slobodnih masnih kiselina (SMK) u lizatu srca. Western blot metodom
određivani su nivoi proteina eNOS, iNOS, NF-κB-p65, pIRS-1/IRS-1,
p85 i p110 subjedinice PI3K, pAkt/Akt, RhoA, AT1R, AT2R, GLUT1,
GLUT4 i CD36 proteina, kao i asocijacija IRS-1 sa p85-PI3K
proteinom u srcu pacova, dok je imunohistohemijskom metodom
određivana ekspresija i lokalizacija eNOS i iNOS. Metodom qRT-PCR
određivan je nivo iRNK za eNOS i iNOS u srcu pacova. Rezultati su
analizirani korišćenjem Studentovog t-testa. Rezultati prikazani u
ovoj doktorskoj disertaciji pokazuju da tretman estradiolom kod
kontrolnih pacova povećava nivo p85 subjedinice PI3K i
fosforilaciju Akt na Thr308, dok smanjuje ekspresiju gena za
iNOS...
Advisors/Committee Members: Isenović, Esma R., 1962-.
Subjects/Keywords: estradiol; heart; eNOS; iNOS; metabolic
substrates
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APA (6th Edition):
Zafirović, Sonja S., 1. (2018). Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zafirović, Sonja S., 1983-. “Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova.” 2018. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zafirović, Sonja S., 1983-. “Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova.” 2018. Web. 18 Jan 2021.
Vancouver:
Zafirović, Sonja S. 1. Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Jan 18].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zafirović, Sonja S. 1. Efekat estradiola na regulaciju endotelne i inducibilne
azot-monoksid-sintaze u srcu gojaznih pacova. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18344/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
7.
Jovanović, Aleksandra A., 1987- 15120743.
Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova.
Degree: Biološki fakultet, 2019, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:19580/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular Endocrinology
Gojaznost je oboljenje povezano sa nizom patoloških stanja kao što su: rezistencija na insulin (IR), kardiovaskularne…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular Endocrinology
Gojaznost je oboljenje povezano sa nizom patoloških
stanja kao što su: rezistencija na insulin (IR), kardiovaskularne
bolesti (KVB) i Diabetes Mellitus tipa 2 (DMT2). Povećana
ekspresija i aktivnost inducibilne azot-monoksid-sintaze (iNOS;
engl. Inducible Nitric Oxide Synthase) u srcu u stanju gojaznosti,
moţe dovesti do apoptoze kardiomiocita i hipertrofije srca, dok sa
druge strane gojaznost zdruţena sa IR doprinosi smanjenoj
aktivnosti Na+/K+-ATPaze, što dovodi do smanjenja kontraktilnosti
vaskulature i razvoja sistemske hipertenzije. Endogeni estradiol
svojim delovanjem sprečava nastanak IR i hiperglikemije i ostvaruje
pozitivne efekte na kardiovaskularni sistem (KVS), ali sinteza i
kardioprotektivni uticaj estradiola mogu biti smanjeni usled
razvoja gojaznosti. Estradiol ostvaruje kardioprotektivne tako što
utiče na smanjenje ekspresije i aktivnosti iNOS, kao i povećanje
ekspresije i aktivnosti Na+/K+-ATPaze, posredstvom različitih
signalnih molekula i kinaza, kao što su: supstrat receptora za
insulin 1 (IRS-1; engl. Insulin Receptor Substrate)/
fosfatidil-inozitol-3-kinaza (PI3K; engl. Phosphatidylinositide
3-Kinase)/ protein kinaza B (Akt; engl. Protein Kinase B),
ekstracelularnim signalima regulisane kinaze 1 i 2 (ERK1/2; engl.
Extracellular Signal-Regulated Protein Kinases 1 i 2) kao i RhoA
(engl. Ras homolog gene family, member A)/ROCK (engl.
Rho-associated protein kinase). Pored direktnog efekta na srce,
estradiol posredno reguliše i njegovu funkciju tako sto utiče na
metabolizam i transport glukoze i SMK, preko transportera glukoze
(GLUT; engl. Glucose Transporters) i translokaze masnih kiselina
(FAT; CD36 ; engl. Fatty Acid Translocase). Za izradu ove doktorske
disertacije je korišćeno 16 adultnih ţenki pacova soja Wistar,
podeljenih u dve eksperimentalne grupe. Prva grupa pacova je tokom
10 nedelja hranjena standardnom laboratorijskom hranom za pacove,
dok je druga grupa pacova tokom 10 nedelja hranjena standardnom
laboratorijskom hranom obogaćenom sa 42% masti (HF reţim ishrane).
Nakon 10 nedelja pacovi su ţrtvovani, sakupljena je krv i izolovan
je serum, a srca su ekstrahovana i delovi tkiva su korišćeni za
izolovanje proteina i RNK. U serumu pacova je odreĎivana
koncentracija estradiola, dok su u lizatu srca pacova odreĎivane
koncentracije L-Arginina (L-Arg), NO i slobodnih masnih kiselina
(SMK). Metodom qRT-PCR odreĎivan je nivo iRNK iNOS u srcu
pacova...
Advisors/Committee Members: Isenović, Esma, 1962- 3563367.
Subjects/Keywords: iNOS; Na+/K+-ATPase; obesity; estradiol;
heart
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APA ·
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APA (6th Edition):
Jovanović, Aleksandra A., 1. 1. (2019). Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:19580/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jovanović, Aleksandra A., 1987- 15120743. “Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova.” 2019. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:19580/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jovanović, Aleksandra A., 1987- 15120743. “Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova.” 2019. Web. 18 Jan 2021.
Vancouver:
Jovanović, Aleksandra A. 11. Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2019. [cited 2021 Jan 18].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:19580/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jovanović, Aleksandra A. 11. Signalni putevi estradiola uključeni u regulaciju
ekspresije i aktivnosti inducibilne azot-monoksid-sintaze i
natrijum-kalijum adenozintrifosfataze u srcu gojaznih ženki
pacova. [Thesis]. Univerzitet u Beogradu; 2019. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:19580/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
8.
Resanović, Ivana, 1986-, 36797031.
Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом.
Degree: Biološki fakultet, 2020, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:22019/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular Endocrinology
Insulin-zavisni dijabetes (IDDM, engl. Insulin-Dependant Diabetes Mellitus) se definiše kao stanje hronične hiperglikemije, odnosno povišne koncentracije…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular Endocrinology
Insulin-zavisni dijabetes (IDDM, engl.
Insulin-Dependant Diabetes Mellitus) se definiše kao stanje
hronične hiperglikemije, odnosno povišne koncentracije glukoze u
krvi uzrokovane poremećajem sekrecije hormona insulina.
Hiperglikemija doprinosi disfunkciji endotela krvnih sudova, što
dovodi do vaskularnih komplikacija i tkivne hipoksije. Uzimajući u
obzir da poremećen balans kiseonika u plazmi ima važnu ulogu u
patogenezi DM, primena terapije kiseonikom pod hiperbaričnim
uslovima (HBK) je preporučena kako bi se usporio razvoj vaskularnih
komplikacija. Terapija HBK ostvaruje antiaterogene, antioksidativne
i kardioprotektivne efekte utičući na promenu nivoa i sastava
masnih kiselina (MK), kao i prenos signala kroz membrane, narušen
hiperglikemijom i hipoksijom. Pokazano je da HBK utiče na signalne
molekule preko kojih insulin ostvaruje dejstvo, kao što su:
supstrat receptora za insulin 1 (IRS-1, engl. Insulin Receptor
Substrate), fosfatidilinozitol 3 kinaza (PI3K, engl.
Phosphatidylinositide 3 Kinase), protein kinaza B (Akt, engl.
Protein Kinase B), kinaze regulisane vanćelijskim signalima 1 i 2
(ERK1/2, engl. Extracellular Signal-Regulated Protein Kinases 1 and
2), nuklearni faktor kappa B (NFκB) i inducibilna
azot-monoksid-sintaza (iNOS, engl. Inducible Nitric Oxide
Synthase), i tako ostvaruje antiinflamatorne efekte kod pacijenata
sa DM. U ovoj doktorskoj disertaciji učestvovalo je 28 pacijenata
kojima je postavljena dijagnoza IDDM sa vaskularnim komplikacijama.
Terapija HBK je podrazumevala izlaganje ispitanika 100% kiseoniku
pod pritiskom do 2,4 apsolutnih atmosfera (ATA) u hiperbaričnoj
komori u trajanju od jednog sata dnevno, tokom 10 tretmana. Pre
početka i nakon tretmana HBK sakupljeni su uzorci krvi ispitanika,
iz kojih su izolovani plazma, serum, i limfociti. Iz uzoraka krvi
pacijenata mereni su nivo HbA1c, glikemija, koncentracija insulina,
lipida, fosfolipida, slobodnih MK (SMK), C-reaktivnog proteina
(CRP), azot-monoksida (NO, engl. Nitric Oxide), homocisteina (Hcy),
kao i aktivnost arginaze. Primenom metode gasne hromatografije
meren je udeo pojedinačnih MK u plazmi...
Advisors/Committee Members: Zarić, Božidarka, biohemičar, 37799271.
Subjects/Keywords: HBO; DM; vascular complications; FA; iNOS
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APA ·
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Export
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APA (6th Edition):
Resanović, Ivana, 1986-, 3. (2020). Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:22019/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Resanović, Ivana, 1986-, 36797031. “Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом.” 2020. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:22019/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Resanović, Ivana, 1986-, 36797031. “Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом.” 2020. Web. 18 Jan 2021.
Vancouver:
Resanović, Ivana, 1986- 3. Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом. [Internet] [Thesis]. Univerzitet u Beogradu; 2020. [cited 2021 Jan 18].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:22019/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Resanović, Ivana, 1986- 3. Утицај терапије кисеоником под хипербаричним условима на
ниво и састав масних киселина у плазми, и регулацију експресије и
активности индуцибилне азот-моноксид-синтазе у лимфоцитима
пацијената са инсулин-зависним дијабетесом. [Thesis]. Univerzitet u Beogradu; 2020. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:22019/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidad de Navarra
9.
[No author].
Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
.
Degree: 2011, Universidad de Navarra
URL: http://hdl.handle.net/10171/17567
► La obesidad consiste en un trastorno en el balance energético que resulta del desequilibrio entre la ingesta calórica y el gasto energético. La leptina,, producto…
(more)
▼ La obesidad consiste en un trastorno en el balance energético que resulta del desequilibrio entre la ingesta calórica y el gasto energético. La leptina,, producto del gen ob, controla el peso corporal disminuyendo la ingesta, fundamentalmente, a través de acciones sobre el hipotálamo y aumentando el gasto energético en el tejido adiposo marrón (TAM). El óxido nítrico (NO) es un potente vasodilatador que también participa en el control de la ingesta y es capaz de regular la función de la grasa parda al identificarse la expresión de la isoforma inducible de la sintasa de óxido nítrico (
iNOS) en los adipositos marrones. Tanto la leptina como la
iNOS participan individualmente en el balance energético. Además, la leptina actúa a través de la
iNOS en diversos procesos biológicos. La hipótesis del presente trabajo consiste en que la relación funcional entre la leptina y la
iNOS se extiende a la modulación del control del balance energético y, consiguientemente, del metabolismo lipídico y glucídico. Tras la obtención del ratón doble knockout (DBKO) carente simultáneamente de ambos genes, se analizó la expresión de moléculas implicadas en la función del TAM. Los ratones DBKO de 12 semanas de edad presentaron una reducción en la ingesta, un menor peso corporal junto con un descenso en los depósitos grasos, una eficiencia en la utilización de alimento disminuida y un aumento de la temperatura rectal respecto a los ratones ob/ob. Asimismo, la deleción del gen de la
iNOS en los ratones ob/ob mejoró el metabolismo glucídico y lipídico. Los ratones DBKO exhibieron una mayor proporción de gotas lipídicas pequeñas y multiloculares que los ratones ob/ob, restaurándose parcialmente las características fenotípicas de la grasa parda. En este contexto, los ratones DBKO presentaron un aumento en la expresión de Prdm16, Pgc-1, sirtuina-1 y 3, Bmp7 y la subunidad Med1 del complejo Mediator, todos ellos implicados en la funcionalidad del TAM y en la activación del programa termogénico. Asimismo, la expresión de Ucp-1 y Ucp-3 se encontró aumentada en el TAM de los ratones DBKO en comparación con los ob/ob. En resumen, la deleción del gen de la
iNOS mejora le balance energético de los ratones ob/ob disminuyendo la eficiencia en la utilización de alimento a través de un aumento en la termogénesis. Estos efectos son mediados, en parte, por cambios moleculares conducentes a una mejoría en la funcionalidad de los adipositos pardos resultantes en la recuperación parcial del fenotipo del TAM.
Advisors/Committee Members: Frühbeck, G. (Gema) (advisor), Gomez-Ambrosi, J. (Javier) (advisor).
Subjects/Keywords: Tejido adiposo marrón;
Termogénesis adaptativa;
Leptina;
iNOS
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APA ·
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APA (6th Edition):
author], [. (2011). Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
. (Thesis). Universidad de Navarra. Retrieved from http://hdl.handle.net/10171/17567
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
author], [No. “Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
.” 2011. Thesis, Universidad de Navarra. Accessed January 18, 2021.
http://hdl.handle.net/10171/17567.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
author], [No. “Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
.” 2011. Web. 18 Jan 2021.
Vancouver:
author] [. Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
. [Internet] [Thesis]. Universidad de Navarra; 2011. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10171/17567.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
author] [. Obtención de un ratón doble knockout de los genes que codifican la isoforma inducible de la sintasa de oxido nítrico y la leptina. Estudio de las consecuencias biológicas derivadas de la ausencia de ambos genes
. [Thesis]. Universidad de Navarra; 2011. Available from: http://hdl.handle.net/10171/17567
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Sartoretto, Simone Marcieli.
Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina.
Degree: PhD, Farmacologia, 2014, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26062014-165520/
;
► No presente estudo, observamos aumentada expressão de iNOS e de proteínas s-nitrosiladas (S-NT) em aorta (AO) e concentração de NO no plasma de ratas diabéticas…
(more)
▼ No presente estudo, observamos aumentada expressão de iNOS e de proteínas s-nitrosiladas (S-NT) em aorta (AO) e concentração de NO no plasma de ratas diabéticas (DB), que foram corrigidas pelo tratamento com insulina (INS), que foi incapaz de normalizar a glicemia. O tratamento com o inibidor da iNOS L-NIL reduziu a expressão de S-NT e a concentração de NO. A contração induzida por agonistas adrenérgicos (ADRs), mas não por cloreto de potássio, que estava reduzida em anéis de AO sem endotélio de ratas DB, foi corrigida pelos tratamentos com INS e L-NIL. A expressão dos receptores de estrógeno ESR2 e GPER estava aumentada em AO de ratas DB e foi corrigida pelo tratamento com INS. Nossos resultados mostram que o aumento da expressão da iNOS/geração de NO é responsável pela redução da contração mediada por receptor ADR, mas não daquela induzida por despolarização direta da membrana. A INS modula negativamente a expressão da iNOS e dos receptores ESR2 e GPER em aorta de ratas DB, efeito que pode contribuir com a restauração da contração induzida por agonistas ADRs.
In the present study, we observed that in aortas (AO) of diabetic (DB) female rats have an increase in iNOS and S-nitrosilated (S-NT) proteins expression along with higher levels of plasmatic NO. Although insulin (INS) treatment did not normalize blood glucose levels, it corrected protein expression and NO concentrations. The iNOS inhibitor treatment reduced the altered expression of S-NT proteins and NO levels. The reduced adrenergic agonists (ADR)-induced contractions in DB without endothelium were corrected by INS and L-NIL treatments, such treatments did not affect the reduced vasoconstriction response to KCl in DB AO. The increase expression of estrogen receptors GPER and ESR2 found in DB AO was recovered by INS treatment. Our results showed that the increased expression of iNOS/NO generation is responsible for reducing ADR-induced contraction, but not for membrane depolarization-induced contraction. INS negatively modulates protein expression of iNOS, ESR2, and GPER receptors in DB AO; such effect may contribute to restore ADR-induced vascular contraction.
Advisors/Committee Members: Akamine, Eliana Hiromi.
Subjects/Keywords: Diabetes; Diabetes; Estrogen receptors; Female; Fêmeas; iNOS; iNOS; Reatividade vascular; Receptores de estrógeno; Vascular reactivity
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APA (6th Edition):
Sartoretto, S. M. (2014). Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26062014-165520/ ;
Chicago Manual of Style (16th Edition):
Sartoretto, Simone Marcieli. “Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26062014-165520/ ;.
MLA Handbook (7th Edition):
Sartoretto, Simone Marcieli. “Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina.” 2014. Web. 18 Jan 2021.
Vancouver:
Sartoretto SM. Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26062014-165520/ ;.
Council of Science Editors:
Sartoretto SM. Avaliação dos mecanismos envolvidos na redução da contração vascular em aortas de ratas diabéticas: papel da iNOS e insulina. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-26062014-165520/ ;
Universitat Rovira i Virgili
11.
Patraca Fierro, José Iván.
Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos.
Degree: Departament de Bioquímica i Biotecnologia, 2015, Universitat Rovira i Virgili
URL: http://hdl.handle.net/10803/348868
► Glia is the group most abundant cells in the brain nervous system, for a long time it was considered only as an element of support…
(more)
▼ Glia is the group most abundant cells in the brain nervous system, for a long time it was considered only as an element of support neuronal unimportant. Hence the need to investigate the behavior and characterization of it in response to the loss of memory and both intracellular signal activation, survival and cognitive impairment.
In mixed culture of mouse cortex C57Bl / 6J, 9 day treatment growth after planting, one hour activation with leptin and prolactin after incubation of cytokines (IFNy, IL-1β and TNF) for 48 hours. Of particular note the connection of signaling molecules activated by oxidative stress as they are p38 and
iNOS MAKP focused.
Neuroprotective effect of adipokines as: Melatonin, prolactin and leptin and its effects on the inhibition of nitric oxide production and
iNOS expression, through p38-MAKP after treatments cytokine inhibiting cell damage astrocyte supporting the anti-inflammatory and antioxidant potential of these compounds, and their potential application in the treatment of specific neurological disorders, suggesting pathways involved in the early stages of Alzheimer's disease.
Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Folch López, Jaume (director), true (authorsendemail).
Subjects/Keywords: Leptina, Prolactina; cèl·lules glials; iNOS, p38-MAPK; leptina, Prolactina; celulas gliales; iNOS, p38-MAPK; Leptin, Prolactin; Glial cells; iNOS, MAPK-p38; 576; 577; 615; 616.8
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APA (6th Edition):
Patraca Fierro, J. I. (2015). Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos. (Thesis). Universitat Rovira i Virgili. Retrieved from http://hdl.handle.net/10803/348868
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Patraca Fierro, José Iván. “Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos.” 2015. Thesis, Universitat Rovira i Virgili. Accessed January 18, 2021.
http://hdl.handle.net/10803/348868.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Patraca Fierro, José Iván. “Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos.” 2015. Web. 18 Jan 2021.
Vancouver:
Patraca Fierro JI. Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos. [Internet] [Thesis]. Universitat Rovira i Virgili; 2015. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10803/348868.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Patraca Fierro JI. Papel de factores endocrinos como melatonina y prolactina, y de adipoquinas como leptina en la modulación de la inflamación asociada a procesos neurodegenerativos. [Thesis]. Universitat Rovira i Virgili; 2015. Available from: http://hdl.handle.net/10803/348868
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Johannes Gutenberg Universität Mainz
12.
Schmidt, Nadine.
Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis.
Degree: 2009, Johannes Gutenberg Universität Mainz
URL: http://ubm.opus.hbz-nrw.de/volltexte/2010/2195/
► An der Entwicklung und Aufrechterhaltung chronisch-inflammatorischer Erkrankungen wie der rheumatoiden Arthritis (RA) ist die Fehlregulation verschiedener pro-inflammatorischer Gene von entscheidender Bedeutung. Bei der RA führt…
(more)
▼ An der Entwicklung und Aufrechterhaltung chronisch-inflammatorischer Erkrankungen wie der rheumatoiden Arthritis (RA) ist die Fehlregulation verschiedener pro-inflammatorischer Gene von entscheidender Bedeutung. Bei der RA führt unter anderem eine erhöhte Expression der induzierbaren NO-Synthase (iNOS) zu einer gesteigerten NO-Produktion, was schließlich zum Knochenabbau beiträgt. Für eine Therapie der RA werden häufig Glukokortikoide eingesetzt, die jedoch viele Nebenwirkungen zeigen. Um eine mögliche Therapiealternative zu identifizieren, sollten die Effekte des anti-inflammatorisch wirksamen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der RA analysiert werden.rnIn humanen C-28/I2-Chondrozyten als in vitro-Modell der RA führte die Inkubation mit einem Zytokingemisch zu einer Induktion der iNOS-Expression, die vom chondrogenen Differenzierungsgrad der Zellen abhängig war. Entscheidend für die iNOS-Induktion in C-28/I2-Zellen ist hauptsächlich der p38-MAPK-, der JAK-STAT- und der NF-kappa B-Signaltransduktionsweg. Eine Inkubation der Zellen mit S-Curvularin führte zu einer deutlichen Hemmung der iNOS-Expression. Dexamethason hatte hingegen keinen Effekt auf die iNOS-Expression, was vermutlich auf die fehlende Expression der Glukokortikoidrezeptor-mRNA zurückgeführt werden kann. Daher können von S-Curvularin abgeleitete Pharmaka möglicherweise auch in Fällen einer Steroidresistenz zur Therapie von RA-Patienten zum Einsatz kommen.rnIm Tiermodell der Kollagen-induzierten Arthritis konnte die anti-inflammatorische Wirkung von S-Curvularin auf mehreren Ebenen bestätigt werden. Die Pilzsubstanz reduzierte sowohl die Schwellung der Pfoten als auch die Expression CII-induzierter pro-inflammatorischer Gene, wie z.B. S100A8, Defb6, Camp und Mpo. Dabei waren die Effekte von S-Curvularin meist deutlicher als in Dexamethason-behandelten Mäusen. Die Analyse von Zytokinen (z.B. TNF-alpha, IL-1beta) und Chemokinen (z.B. MCP-1, MIP-1alpha) zeigte, dass die CII-induzierte Expression dieser pro-inflammatorischen Mediatoren in den Pfoten der Mäuse durch eine Therapie mit S-Curvularin und Dexamethason wieder reduziert werden konnte, wobei Unterschiede zwischen den Behandlungen beobachtet werden konnte.rnAuch im Tiermodell der LPS-induzierten akuten Entzündung wurde die iNOS- und die S100A8-Expression in verschiedenen Geweben S-Curvularin reduziert. rnrnS-Curvularin ist also in der Lage, in verschiedenen Modellen der RA und im akuten Entzündungsmodell die pro-inflammatorische Genexpression effizient zu hemmen und könnte somit in Zukunft eine Rolle in der Therapie der RA einnehmen.rn
Dysregulation of the expression of several pro-inflammatory genes is involved in the development and progression of chronic inflammatory diseases such as rheumatoid arthritis (RA). In RA, enhanced expression of inducible nitric oxide synthase (iNOS) leads to inappropriate NO production and thereby contributes to joint destruction. Glucocorticoids, often used for the treatment of RA, display severe side effects. To identify new possibilities for…
Subjects/Keywords: Inflammation; iNOS; Chondrozyten; S-Curvularin; rheumatoide Arthritis; inflammation; iNOS; chondrocytes; S-Curvularin; rheumatoid arthritis; Life sciences
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APA ·
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APA (6th Edition):
Schmidt, N. (2009). Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2010/2195/
Chicago Manual of Style (16th Edition):
Schmidt, Nadine. “Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis.” 2009. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 18, 2021.
http://ubm.opus.hbz-nrw.de/volltexte/2010/2195/.
MLA Handbook (7th Edition):
Schmidt, Nadine. “Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis.” 2009. Web. 18 Jan 2021.
Vancouver:
Schmidt N. Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2009. [cited 2021 Jan 18].
Available from: http://ubm.opus.hbz-nrw.de/volltexte/2010/2195/.
Council of Science Editors:
Schmidt N. Analyse des anti-inflammatorischen Pilzmetaboliten S-Curvularin in verschiedenen Modellen der rheumatoiden Arthritis. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2009. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2010/2195/
Johannes Gutenberg Universität Mainz
13.
Casper, Ingrid.
Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen.
Degree: 2013, Johannes Gutenberg Universität Mainz
URL: http://ubm.opus.hbz-nrw.de/volltexte/2013/3476/
► Die humane induzierbare NO-Synthase (iNOS) spielt bei zahlreichen Erkrankungen wie Asthma, Krebs und der rheumatoiden Arthritis eine entscheidende Rolle. Durch Fehlregulation der iNOS-Expression kommt es…
(more)
▼ Die humane induzierbare NO-Synthase (iNOS) spielt bei zahlreichen Erkrankungen wie Asthma, Krebs und der rheumatoiden Arthritis eine entscheidende Rolle. Durch Fehlregulation der iNOS-Expression kommt es häufig zu massiven Gewebeschädigungen. Aus diesem Grund ist es wichtig die Mechanismen der Genregulation der iNOS-Expression zu verstehen. Bei Affinitätschromatographie-Analysen wurde das zytosolische PolyA-bindende Protein (PABP) als direkter Interaktionspartner der 3´UTR der humanen iNOS identifiziert. Weitere Bindungsanalysen konnten eine spezifische Bindestelle für PABP in der 5´UTR und zwei Bindestellen im AU-reichen Bereich der 3´UTR der humanen iNOS nachweisen. Eine siRNA-mediierte Herabregulation von PABP mit Hilfe der stabilen Expression spezifischer siRNAs in DLD-1 Zellen (siPABP Zellen) zeigte eine signifikant verringerte Expression der humanen iNOS und damit einhergehend eine verringerte NO-Produktion nach Zytokinstimulation. Promotoranalysen zeigten keine Veränderung der Induzierbarkeit des humanen 16 kb iNOS-Promotors in siPABP Zellen. RNA-Stabilitätsanalysen zeigten einen verstärkten Abbau der iNOS-mRNA in diesen Zellen, so dass davon auszugehen ist, dass die Regulation der humanen iNOS über die mRNA-Stabilität erfolgt. Reportergen-Analysen mit Plasmiden, welche die 5’ und/oder 3’UTR Sequenzen der humanen iNOS mit den identifizierten PABP-Bindestellen oder Mutationen in diesen Bindestellen enthielten, zeigten, dass PABP die iNOS-mRNA über die 5´UTR stabilisiert und anscheinend über die 3´UTR einen destabilisierenden Effekt auf die mRNA ausübt. Ebenfalls scheint PABP über die 3’UTR dieTranslation der iNOS mRNA zu hemmen. Die Ergebnisse dieser Arbeit zeigen, dass PABP, über seine allgemeinen Funktionen hinaus, eine spezifische Rolle in der Regulation der Expression der humanen iNOS einnimmt.rnDie rheumatoide Arthritis (RA) ist eine chronisch entzündliche Autoimmunerkrankung, welche überwiegend die peripheren Gelenke der Hände und Füße betrifft. Die aktuellen Therapiemöglichkeiten sind immer noch mit einer Vielzahl von Nebenwirkungen behaftet und führen nicht zur vollständigen Remission der Erkrankung, so dass die Entwicklung neuer Medikamente unerlässlich ist. In dieser Arbeit wurden die antiinflammatorischen Substanzen Gallielalacton (Gal) und Oxacyclododecindion (Oxa) im Mausmodell der kollagen-induzierten Arthritis (CIA) getestet. Leider waren beide Substanzen nicht in der Lage die Symptome der CIA zu vermindern, obwohl beide im Modell der LPS-induzierten akuten Entzündung die Expression proinflammatorischer Mediatoren senken konnten. Die Substanz S-Curvularin (SC) hat sich im CIA-Modell bereits bewährt und wurde in dieser Arbeit weiter untersucht. SC war in der Lage die Expression knorpel- und knochendestruktiver Markergene signifikant zu verrindern. rnIn der vorliegenden Arbeit wurden neue microRNAs identifiziert, die in der Pathogenese der CIA eine Dysregulation zeigen. Die Expression dieser microRNAs wurde von SC wieder auf das Normalniveau gebracht, so dass SC eine vielversprechende Substanz in…
Subjects/Keywords: post-transkriptionelle Regulationsmechanismen; PABP; iNOS; rheumatoide Arthritis; CIA; post-transcriptional regulation; PABP; iNOS; rheumatoid arthritis; CIA; Life sciences
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Casper, I. (2013). Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen. (Doctoral Dissertation). Johannes Gutenberg Universität Mainz. Retrieved from http://ubm.opus.hbz-nrw.de/volltexte/2013/3476/
Chicago Manual of Style (16th Edition):
Casper, Ingrid. “Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen.” 2013. Doctoral Dissertation, Johannes Gutenberg Universität Mainz. Accessed January 18, 2021.
http://ubm.opus.hbz-nrw.de/volltexte/2013/3476/.
MLA Handbook (7th Edition):
Casper, Ingrid. “Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen.” 2013. Web. 18 Jan 2021.
Vancouver:
Casper I. Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen. [Internet] [Doctoral dissertation]. Johannes Gutenberg Universität Mainz; 2013. [cited 2021 Jan 18].
Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3476/.
Council of Science Editors:
Casper I. Analysen zur Expression proinflammatorischer Mediatoren in humanen Zellkulturmodellen und murinen Tiermodellen chronisch-inflammatorischer Erkrankungen. [Doctoral Dissertation]. Johannes Gutenberg Universität Mainz; 2013. Available from: http://ubm.opus.hbz-nrw.de/volltexte/2013/3476/
14.
Souza, Flavia Castro Ribas de.
Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode.
Degree: Mestrado, Fisiopatologia Experimental, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-25052012-173836/
;
► Introdução: Estima-se que 10% dos doentes com asma tm sintomas e limitaes importantes, como exacerbaes freqentes ou reduo persistente da funo respirat
ria As alteraes do…
(more)
▼ Introdução: Estima-se que 10% dos doentes com asma tm sintomas e limitaes importantes, como exacerbaes freqentes ou reduo persistente da funo respirat
ria As alteraes do parnquima pulmonar distal tem sido recentemente abordadas na fisiopatologia da asma. Apesar do uso de corticoster
ides, pacientes com asma refratria tm mais estresse oxidativo, assim como apresentam ativaao da
iNOS. Alm disso, muitos dos dispositivos utilizados para administrao de ester
ides inalat
rios geram partculas que no chegam efetivamente s vias areas distais e ao parnquima pulmonar. Objetivos: Avaliamos os efeitos do tratamento com montelucaste ou dexametasona tratamentos associados ou no a um inibidor especfio da
iNOS (1400W) na resposta eosinoflica, remodelamento da matriz extracelular, estresse oxidativo, contedo de actina, clulas positivas para IL4, IL5, MMP9, TIMP1, IFN, TGF do parnquima em cobaias com inflamao crnica pulmonar. Métodos: As cobaias foram inaladas com ovalbumina (grupo OVA) 2X/semana por 4semanas. Ap
s a 4 inalao, as cobaias foram tratadas diariamente com montelucaste (grupo OVAM 10mg/Kg/PO/dia) ou dexametasona (grupo OVAD 5mg/Kg/IP/dia). O inibidor da
iNOS, 1400W (grupo OVAW 1mg/kg/dia) foi administrado intraperitonealmente nos ltimos 4 dias (OVAW, OVADW e grupos OVAMW). Ap
s 72 horas da 7 inalao, as cobaias foram anestesiadas, e os fragmentos de tecido pulmonar distal foram submetidos avaliao histopatol
gica. Resultados: Houve um aumento no infiltrado eosinoflco, nas clulas positivas para IL4, IL5, TIMP1, MMP9,
iNOS, IFN TGF, contedo de actina, isoprostano PGF2 alfa, fibras colgenas e elsticas nos animais OVA em comparao com animais SAL (p<0,05). Houve uma diminuio no nmero de eosin
filos, clulas positivas para IL4, IL5, MMP9, TIMP1, IFN, TGF, contedo de actina, colgeno e isoprostano PGF2 alfa em todos os grupos tratados em comparao com animais OVA (p<0,05). O contedo de fibras elsticas foram reduzidas somente nos grupos OVAMW, OVADW e OVAW em comparao com animais OVA (p<0,05). A associao de 1400W e o tratamento com montelucaste (grupo OVAMW) potencializou a reduo do contedo de actina, fibras elsticas, isoprostano PGF2 alfa de clulas positivas para IL4, IL5, TIMP1, IFN TGF e
iNOS em relao ao grupo montelucaste (OVAM) (p<0,05). Os tratamentos com 1400W e dexametasona (grupo OVADW) contriburam para uma maior reduo do contedo das fibras elsticas, actina e isoprostanoPGF2 alfa e o nmero de clulas positivas para IL4, IL5, IFN e TIMP1 em relao ao grupo dexametasona (OVAD) (p<0,05). Conclusões: O tratamento com corticoster
ides associados inibio da
iNOS contribuiu para uma maior reduo da remodelao da matriz extracelular, diminuiu o estresse oxidativo, e tambm foi eficiente para atenuar a resposta inflamat
ria Th2 no …
Advisors/Committee Members: Tibério, Iolanda de Fátima Lopes Calvo.
Subjects/Keywords: Anti-leucotrienos; Antileukotrienes; Asma; Asthma; Chronic allergic inflammation; Estresse oxidativo; Glicocorticóides; Glucocorticoids; Inflamação alérgica crônica; iNOS; iNOS; Oxidative stress
Record Details
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Share »
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Similar Records
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« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Souza, F. C. R. d. (2012). Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5160/tde-25052012-173836/ ;
Chicago Manual of Style (16th Edition):
Souza, Flavia Castro Ribas de. “Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode.” 2012. Masters Thesis, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5160/tde-25052012-173836/ ;.
MLA Handbook (7th Edition):
Souza, Flavia Castro Ribas de. “Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode.” 2012. Web. 18 Jan 2021.
Vancouver:
Souza FCRd. Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-25052012-173836/ ;.
Council of Science Editors:
Souza FCRd. Efeitos dos tratamentos com glicocorticóides, com antagonista do receptor do cisteinil-leucotrieno D4 e com o inibidor específico da iNOS na resposta inflamatória e de remodelamento no tecido pulmonar periférico em mode. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/5/5160/tde-25052012-173836/ ;
15.
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa.
Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.
Degree: Mestrado, Processos Inflamatórios e Alérgicos, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/
;
► Introdução: A importância do parênquima pulmonar na fisiopatologia da asma tem sido recentemente enfatizada, particularmente nos pacientes com asma grave e de difícil controle. O…
(more)
▼ Introdução: A importância do parênquima pulmonar na fisiopatologia da asma tem sido recentemente enfatizada, particularmente nos pacientes com asma grave e de difícil controle. O óxido nítrico (NO) é um importante modulador da resposta contrátil, inflamatória e de remodelamento pulmonar que ocorrem na asma. Embora seu papel na modulação em vias aéreas proximais e distais já tenha sido estabelecido, seus efeitos no parênquima pulmonar foram pouco investigados. Objetivos: Avaliar se a inibição da arginase 2, por intermédio do tratamento com N-hydroxy-nor-L-arginine (nor-NOHA), e da
iNOS, por intermédio da administração de 1400W, pode modular a resposta constritora de parênquima distal, o estresse oxidativo, a expressão de
iNOS, assim como a atividade da arginase, em modelo de inflamação alérgica crônica pulmonar em cobaias. Métodos: Os animais foram expostos a sete inalações com soro fisiológico ou com ovoalbumina em doses crescentes (1~5mg/ml- 4 semanas) e tratados com 1400W (2mg/Kg ip. diariamente) iniciando após a 7ª inalação e nor- NOHA (10M - infusão no banho durante a avaliação da mecânica oscilatória), ou associação de 1400W + nor-NOHA (administrado conforme descrito anteriormente). Setenta e duas horas após a sétima inalação os animais foram anestesiados, exsanguinados e a foi realizada a avaliação da mecânica oscilatória do parênquima pulmonar, sendo obtidos os valores de resistência e elastância tecidual na condição basal e após desafio (0,1% de ovoalbumina). Em seguida, os fragmentos de tecido pulmonar periférico foram fixados em solução de formolaldeído a 4%, por 48 horas. Depois de terminada a fixação, o material foi submetido às técnicas histológicas habituais com parafina, para obtenção de cortes de 4m de espessura. Os cortes foram corados para Hematoxilina e Eosina e utilizando técnica de imunohistoquímica foram avaliados o número de células
iNOS positivas, a expressão de PGF2, do fator de transcrição NF-kB e de arginase 2 no septo alveolar, por intermédio de avaliação morfométrica. A expressão de arginase 2, NF-kB e PGF2 nos fragmentos de tecido pulmonar periférico foram avaliados em aumento 400X utilizando o programa Image-Pro Plus 4.5v Image Analysis System e os resultados foram expressos em porcentagem. Em relação ao número de células
iNOS positivas nos fragmentos pulmonares, foi utilizada a técnica de contagem de pontos, determinada pelo número de pontos que coincidiam em células positivas em cada campo dividido pelo número de pontos que incidiam no tecido pulmonar, no aumento de 1000x. Foram analisados 10 campos por corte, selecionados de forma randômica. Os resultados foram expressos como células por unidade de área (104m2). Para a avaliação da atividade da arginase 2, foi utilizado o método descrito no kit da BioAssay Systems que utiliza um cromogênio, que forma um complexo corado com uréia, produzido na reação de arginase. A determinação da atividade da arginase 2 envolveu a medida da velocidade de reação, expressa em termos de atividade por miligrama de proteína (U/mg). Resultados: Não houve…
Advisors/Committee Members: Tibério, Iolanda de Fátima Lopes Calvo.
Subjects/Keywords: Arginase; Arginase; Chronic allergic inflammation; Estresse oxidativo; Inflamação alérgica crônica; iNOS; iNOS; NF-kB; NF-kB; Oxidative stress
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APA (6th Edition):
Aristóteles, L. R. d. C. R. B. (2012). Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;
Chicago Manual of Style (16th Edition):
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa. “Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.” 2012. Masters Thesis, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;.
MLA Handbook (7th Edition):
Aristóteles, Luciana Ritha de Cassia Rolim Barbosa. “Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar.” 2012. Web. 18 Jan 2021.
Vancouver:
Aristóteles LRdCRB. Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;.
Council of Science Editors:
Aristóteles LRdCRB. Modulação da mecânica do tecido pulmonar periférico e da resposta do estresse oxidativo pela inibição da arginase e da iNOS em modelo experimental de inflamação crônica pulmonar. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/5/5165/tde-31072012-113923/ ;
16.
Lima, Carina Buzzo de.
Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1.
Degree: PhD, Imunologia, 2014, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-093448/
;
► O reconhecimento da flagelina é compartilhado pelo receptor transmembrânico TLR5 e citosólico NAIP5/NLRC4. Entretanto, pouco se sabe sobre os mecanismos efetores individuais induzidos a partir…
(more)
▼ O reconhecimento da flagelina é compartilhado pelo receptor transmembrânico TLR5 e citosólico NAIP5/NLRC4. Entretanto, pouco se sabe sobre os mecanismos efetores individuais induzidos a partir do reconhecimento extra e intracelular da flagelina. Aqui, nós demonstramos que macrófagos estimulados com a flagelina citosólica (FLA-BSDot) induziu a expressão de iNOS, enzima responsável pela produção do óxido nítrico (NO). A expressão de iNOS foi dependente do eixo NAIP5/NLRC4/caspase-1 e independente de IL-1β, IL-18 e MyD88, descartando a via de ativação dos TLRs. Ainda, esta via não requer a ativação do fator de transcrição IRF-1, mas envolve a ativação do NF-kB, assim como a clivagem da enzima PARP-1 (poly(ADP-ribose)polymerase-1). Por fim, avaliamos a relevância biológica desta via no controle das infecções por L. pneumophila e S. Typhimurium, dados que definem um mecanismo efetor adicional no controle de patógenos.
Recognition of flagellin is shared by transmembranic TLR5 and cytosolic NAIP5/NLRC4. However, little is known about the individual effector mechanisms induced by extra and intracellular flagellin. Here, we have demonstrated that cytosolic flagellin-stimulated macrophages (FLA-BSDot) induced iNOS expression, an enzyme responsible for the production of nitric oxide (NO). iNOS expression was dependent of the NAIP5/NLRC4/caspase-1 axis and independent of IL-1β, IL-18 and MyD88, discarding TLRs signaling pathway. Still, this pathway do not require the activation of IRF-1 transcriptional factor, but involves NF-kB activation as well as the cleavage of the enzyme, PARP-1 (poly(ADP-ribose)polymerase-1). Finally, we have evaluated the biological relevance of this pathway in the control of the infections by L. pneumophila e S. Typhimurium, which define an additional effector mechanism to the control of pathogens.
Advisors/Committee Members: Bortoluci, Karina Ramalho.
Subjects/Keywords: Caspase-1; Caspase-1; Inflamassomas; Inflammasome; iNOS; iNOS; NF-kB; NF-kB; PARP-1; PARP-1
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APA (6th Edition):
Lima, C. B. d. (2014). Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-093448/ ;
Chicago Manual of Style (16th Edition):
Lima, Carina Buzzo de. “Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 18, 2021.
http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-093448/ ;.
MLA Handbook (7th Edition):
Lima, Carina Buzzo de. “Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1.” 2014. Web. 18 Jan 2021.
Vancouver:
Lima CBd. Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 18].
Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-093448/ ;.
Council of Science Editors:
Lima CBd. Avaliação dos mecanismos moleculares envolvidos na expressão de iNOS mediada pelo eixo NAIP5/NLRC4-Caspase-1. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11072014-093448/ ;
17.
Dobutović Branislava.
The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver.
Degree: PhD, Biology, 2013, University of Belgrade
URL: http://dx.doi.org/10.2298/BG20130712DOBUTOVIC
;
http://eteze.bg.ac.rs/application/showtheses?thesesId=762
;
https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get
;
http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579506
► In recent years, there have been prominent findings suggesting that ghrelin has antioxidant and anti-inflammatory effects. In this study we investigated the effects of ghrelin…
(more)
▼ In recent years, there have been prominent findings suggesting that ghrelin has antioxidant and anti-inflammatory effects. In this study we investigated the effects of ghrelin on protein expression of liver antioxidant enzymes superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) as well as protein expression of their upstream regulator, nuclear factor kappa B (NFκB). We have also studied the effects of ghrelin on liver inducible nitric oxide synthase (iNOS) protein expression. Furthermore, we aimed to investigate whether extracellular regulated protein kinase (ERK 1/2) and protein kinase B (Akt) are involved in ghrelin regulated liver antioxidant enzymes and iNOS protein expression. Male Wistar rats (200-250g) were treated with ghrelin (0,3nmol/5μl) injected into the lateral cerebral ventricle every 24 h for 5 day, 2 h after the last treatment the animals were sacrificed and liver excised. For protein expression of antioxidant enzymes, iNOS, phosphorylation of Akt at Ser473, ERK1/2 and nuclear factor B (NFκB) subunits 50 and 65, Western blot method was used. Results show significantly higher protein expression of Cu/Zn SOD (p<0,05), Mn-SOD (p<0,05), CAT (p<0,01), GPx, (p<0,001), and GR (p<0,01) in the liver isolated from ghrelin treated animals compared with control animals. On the contrary, ghrelin significantly (p<0,001) reduced protein expression of iNOS. In addition, phosphorylation of NFκB subunits p65 and p50 were significantly (p<0,05) decreased by ghrelin when compared with controls. Phosphorylation of ERK1/2 and phosphorylation of Akt at Ser473 were significantly higher in ghrelin treated than in control animals (p<0,05 for ERK1/2; p<0,01 for Akt). In conclusion, results from this study show that activation of Akt and ERK1/2 are involved in ghrelin-mediated regulation of protein expression of antioxidant enzymes and iNOS in the rat liver.
U poslednjih nekoliko godina, objavljeni naučni podaci sve više ukazuju na antioksidantne i antiinflamatorne efekte grelina. U ovoj doktorskoj disertaciji su izučavani efekti grelina na ekspresiju enzima jetre: superoksid dismutaze (engl. Superoxide Dismutase, SOD), katalaze (engl. Catalase, CAT), glutation peroksidaze (engl. Glutathione peroxidase, GPx) i glutation reduktaze (engl. Glutathione reductase, GR), kao i ekspresiju njihovog uzvodnog regulatora, nuklearnog faktora-kB (engl. Nuclear factor- kappa B, NFκB). Takođe su izučavani efekti grelina na regulaciju enzima inducibilne azot-monoksid-sintaze (engl. Inducible nitric oxide synthase, iNOS; NOS2) u jetri pacova. Jedan od ciljeva istraživanja u okviru ove doktorske disertacije je bio i utvrđivanje uloge kinaze regulisane ekstraćelijskim signalima (engl. Extracellular regulated protein kinase, ERK1/2) i protein kinaze B (engl. Protein kinase B, Akt) u grelinom regulisonoj aktivnosti i ekspresiji antioksidatnivnih enzima i iNOS u jetri pacova. Kontrolni, normalno hranjeni mužjaci pacova, Wistar soja (mase 200-250g) kao i mužjaci pacova…
Subjects/Keywords: ghrelin; Akt; ERK1/2; iNOS; antioxidative ensymes; NFκB; Liver; grelin; Akt; ERK 1/2; iNOS; antioksidativni enzimi; NFκB; Jetra
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APA ·
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APA (6th Edition):
Branislava, D. (2013). The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver. (Doctoral Dissertation). University of Belgrade. Retrieved from http://dx.doi.org/10.2298/BG20130712DOBUTOVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=762 ; https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579506
Chicago Manual of Style (16th Edition):
Branislava, Dobutović. “The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver.” 2013. Doctoral Dissertation, University of Belgrade. Accessed January 18, 2021.
http://dx.doi.org/10.2298/BG20130712DOBUTOVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=762 ; https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579506.
MLA Handbook (7th Edition):
Branislava, Dobutović. “The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver.” 2013. Web. 18 Jan 2021.
Vancouver:
Branislava D. The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver. [Internet] [Doctoral dissertation]. University of Belgrade; 2013. [cited 2021 Jan 18].
Available from: http://dx.doi.org/10.2298/BG20130712DOBUTOVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=762 ; https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579506.
Council of Science Editors:
Branislava D. The effect of ghrelin on the regulation of antioxidantenzymes and inducible nitric oxide synthase in rat liver. [Doctoral Dissertation]. University of Belgrade; 2013. Available from: http://dx.doi.org/10.2298/BG20130712DOBUTOVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=762 ; https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579506
18.
Cabrié, Aimeric.
Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2017, Université Paris-Saclay (ComUE)
URL: http://www.theses.fr/2017SACLS397
► Le monoxyde d’azote (NO) est une molécule gazeuse synthétisée par les NO Synthases à partir de L-arginine. NO est une puissante molécule de signalisation dans…
(more)
▼ Le monoxyde d’azote (NO) est une molécule gazeuse synthétisée par les NO Synthases à partir de L-arginine. NO est une puissante molécule de signalisation dans de nombreux processus physiologiques comme la vasodilatation et la neurotransmission. Il module l’activité de multiples protéines (ex : guanylate cyclase soluble et ribonucléotide réductase) grâce à la nitrosylation de groupements thiol ou de métaux de transition. En tant que radical libre, NO peut réagir avec de nombreuses espèces comme l’oxygène moléculaire, et ainsi former des dérivés réactifs. Grâce à ces propriétés, NO est un acteur majeur de l’immunité innée et de l’inflammation. Les phagocytes produisent de grandes quantités de NO en réponse à des stimuli proinflammatoires, via l’activité NO Synthase inductible (iNOS). En raison des effets délétères des dérivés de NO, l’activité iNOS doit être finement régulée. Le suppresseur de tumeur p53 est capable de réprimer l’expression du gène Nos2 après avoir été lui-même activé en réponse à une accumulation de NO. La protéine p73 est un homologue de p53 encodé par un gène qui génère à la fois des isoformes actives (TAp73) et des isoformes qui sont dépourvues du domaine de transactivation N-terminal et exercent un effet dominant négatif (ΔNp73). Cette étude se focalise sur le rôle des isoformes TAp73 dans la régulation de l’expression de la iNOS. Nous démontrons que les isoformes TAp73 régulent négativement l’expression de la iNOS aux niveaux transcriptionnel et post-traductionnel en potentialisant l’effet répresseur du TGF-β, ce qui résulte en une forte surexpression de la iNOS dans les cellules TAp73-/-. Ces résultats confortent le rôle de la famille p53 comme un réseau essentiel de protéines régulatrices des fonctions du TGF-β.
Nitric oxide (NO) is a gaseous molecule synthesized from L-arginine by Nitric Oxide Synthases. NO acts as a potent signaling molecule in various physiological processes like vasorelaxation and neurotransmission. It modulates the activity of many proteins (e.g. soluble guanylate cyclase and ribonucleotide reductase) through nitrosylation of thiol moieties or transition metal ions. As a free radical, NO can also react with a number of cellular species, notably molecular oxygen, to form reactive oxygen species and reactive nitrogen species. Thanks to these properties, NO appears as a major component of innate immune response and inflammation. Phagocytes produce large amounts of NO in response to proinflammatory through inducible Nitric Oxide Synthase (iNOS) activity. Because of the harmful effects of NO derivatives on cellular components, iNOS activity needs to be tightly regulated. The p53 tumor suppressor has been shown to repress Nos2 after being activated by NO itself. The p73 protein is an homologous encoded by the TP73 gene that generate transcriptionally active TAp73 isoforms and ΔNp73 isoforms that lack the transactivation domain and exert a dominant negative effect. This study focuses on the role of TAp73 isoforms in regulation of iNOS expression. We demonstrate that TAp73…
Advisors/Committee Members: Lepoivre, Michel (thesis director).
Subjects/Keywords: Monoxyde d’azote (NO); Inos; P73; TGF-β; Nrf2; Nitric oxide (NO); Inos; P73; TGF-β; Nrf2
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APA ·
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Export
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APA (6th Edition):
Cabrié, A. (2017). Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2017SACLS397
Chicago Manual of Style (16th Edition):
Cabrié, Aimeric. “Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression.” 2017. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed January 18, 2021.
http://www.theses.fr/2017SACLS397.
MLA Handbook (7th Edition):
Cabrié, Aimeric. “Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression.” 2017. Web. 18 Jan 2021.
Vancouver:
Cabrié A. Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2017. [cited 2021 Jan 18].
Available from: http://www.theses.fr/2017SACLS397.
Council of Science Editors:
Cabrié A. Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible : TAp73 Isoforms and TGF-β Signaling Cooperate to Suppress Inducible Nitric Oxide Synthase Expression. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2017. Available from: http://www.theses.fr/2017SACLS397
NSYSU
19.
Chen, Chia-Jung.
The Activation of Erks in Intestine and Lung of Thermal Injured-rats.
Degree: Master, Biological Sciences, 2003, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-210826
► Burn-induced intestinal barrier failure has been proposed to be a potential cause of subsequent multiple organ failure after burn. Studies have shown that the increased…
(more)
▼ Burn-induced intestinal barrier failure has been proposed to be a potential cause of subsequent multiple organ failure after burn. Studies have shown that the increased
iNOS activity is closely related to intestinal and pulmonary damage in rats after burn. Expression of
iNOS and MMP-9 is regulated by nuclear factor NF-κB activation, which is frequently a result of MAPKs pathway activation. This study was to investigate the role of ERKs in intestinal and pulmonary damage induced by burn in rats. In experiments, SD rats underwent 30 ~ 35 % TBSA burn. At various times after burn, intestinal mucosa and pulmonary proteins were assayed for ERKs and p38 phosphorylation by immunoblotting, nuclear extracts were assayed for NF-κB activation by EMSA, intestinal and pulmonary
iNOS, MMP-9 expressions were evaluated by RT-PCR, the FITC-dextran permeability was determined to assess the intestinal barrier function and the pulmonary microvascular dysfunction was quantitated by measuring the extravasation of Evans blue dye. The results show that burn induced ERKs and p38 phosphorylation, the expression of
iNOS, and NF-κB activation in intestinal mucosa and lung, but the expression of MMP-9 was attenuated. Treatment with MEK1/2 inhibitors, PD98059 (10 mg/kg i.p.) or U0126 (5 mg/kg i.p.) immediately after burn, attenuated the phosphorylation of intestinal mucosa and pulmonary ERKs, the activation of NF-κB, the increase in intestinal permeability, and pulmonary microvascular dysfunction. Interestingly, the expression of
iNOS in intestinal mucosa and pulmonary tissues was induced by PD98059 administration, but the expression of MMP-9 in intestinal mucosa was attenuated by PD98059 administration. These results suggest that the tissue damage is regulated by NF-κB activation and the activation of NF-κB is primarily mediated by signal pathway of ERKs in burn-injured rats, so the signal transduction pathway may involve ERKs and p38, NF-κB,
iNOS or MMP-9, then causes tissue damage. Further, burn-induced intestinal mucosa and pulmonary ERKs have different degree of activation. The p38 and ERKs phosphorylation showed a two-step activation in intestinal mucosa and pulmonary tissues after burn. Inhibition of intestinal and pulmonary ERKs in vivo afforded significant protection against burn-induced barrier failure. However, the data showed that
iNOS may not play a major role in the burn-induced intestinal and pulmonary damage, and MMP-9 may have more affect on tissues damage.
Advisors/Committee Members: Lee-Wei Chen (chair), none (chair), Ching-Mei Hsu (committee member).
Subjects/Keywords: iNOS; tissue damage; ERKs burn
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Chen, C. (2003). The Activation of Erks in Intestine and Lung of Thermal Injured-rats. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-210826
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Chia-Jung. “The Activation of Erks in Intestine and Lung of Thermal Injured-rats.” 2003. Thesis, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-210826.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Chia-Jung. “The Activation of Erks in Intestine and Lung of Thermal Injured-rats.” 2003. Web. 18 Jan 2021.
Vancouver:
Chen C. The Activation of Erks in Intestine and Lung of Thermal Injured-rats. [Internet] [Thesis]. NSYSU; 2003. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-210826.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen C. The Activation of Erks in Intestine and Lung of Thermal Injured-rats. [Thesis]. NSYSU; 2003. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728103-210826
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
20.
Su, Yin-di.
Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp.
Degree: PhD, Marine Biotechnology and Resources, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0111116-204915
► Studies on the secondary metabolites of the octocoral Briareum sp. collected off the coast of Southern Taiwan had led to the isolation of twenty-four new…
(more)
▼ Studies on the secondary metabolites of the octocoral Briareum sp. collected off the coast of Southern Taiwan had led to the isolation of twenty-four new briarane analogues, including briarenolides HâY (1â18) and ZIâZVI (19â24), along with thirteen known compounds, briaexcavatolides H (25), F (28), G (29), P (32), solenolides A (26), C (27), brianolide (30), briaexcavatins P (31), K (34), N (35), excavatolides M (33), Z (36), and 2β-acetoxy-2-(debutyryloxy) stecholide E (37). It is worth noting that briarenolide J (3) is the first dichlorobriarane found to possess a chlorine atom at C-12. In addition, one new steroid pinnigorsterol A (38) and two new 9,11-secosterols pinnigorsterol B (39) and pinnisterol A (40) were obtained from a gorgonian identified as Pinnigorgia sp. The structures of metabolites 1â40 were established by spectroscopic methods and by comparison of the spectroscopic data with those of related metabolites.
In the in vitro anti-inflammatory experiments, metabolites 3, 5, 39, and 40 have displayed significant inhibitory effects on superoxide anion generation (IC50 = 7.73, 7.14, 3.12, and 1.70 μg/mL) and elastase release (IC50 = 5.14, 6.86, 2.40, and 1.02 μg/mL) by human neutrophils. Sterol 38 showed an inhibitory effect on human neutrophil elastase release (IC50 = 4.47 μg/mL). Furthermore, compounds 9, 15 and 38â40 were found to inhibit the accumulation of the pro-inflammatory
iNOS protein (47.3, 41.9, 16.3, 14.5, and 19.8%) and COX-2 protein (35.6, 26.1, 37.1, 44.4, and 59.0%) in the LPS- stimulated RAW264.7 macrophage cells.
Advisors/Committee Members: Ping-Jyun Sung (committee member), Yao-Haur Kuo (chair), Fang-Rong Chang (chair), Tzong-Huei Lee (chair), Yang-Chang Wu (chair), Jyh-Horng Sheu (committee member).
Subjects/Keywords: Pinnigorgia; Briareum; superoxide anion; elastase; iNOS; COX-2
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APA (6th Edition):
Su, Y. (2016). Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0111116-204915
Chicago Manual of Style (16th Edition):
Su, Yin-di. “Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp.” 2016. Doctoral Dissertation, NSYSU. Accessed January 18, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0111116-204915.
MLA Handbook (7th Edition):
Su, Yin-di. “Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp.” 2016. Web. 18 Jan 2021.
Vancouver:
Su Y. Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp. [Internet] [Doctoral dissertation]. NSYSU; 2016. [cited 2021 Jan 18].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0111116-204915.
Council of Science Editors:
Su Y. Studies on the Chemical Constituents and Their Biological Activities from the Formosan Corals Briareum sp. and Pinnigorgia sp. [Doctoral Dissertation]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0111116-204915
21.
Hori, Kazuichiro.
Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb.
Degree: 博士(医学), 2017, Mie University / 三重大学
URL: http://hdl.handle.net/10076/14575
► Background: Studies have shown that ischemia-reperfusion (I/R) produces free radicals leading to lipid peroxidation and damage to skeletal muscle. The purposes of this study were…
(more)
▼ Background: Studies have shown that ischemia-reperfusion (I/R) produces free radicals leading to lipid peroxidation and damage to skeletal muscle. The purposes of this study were 1) to assess the histological findings of gastrocnemius muscle (GC) and tibialis anterior muscle (TA) in I/R injury model mice, 2) to histologically analyze whether a single pretreatment of edaravone inhibits I/R injury to skeletal muscle in murine models and 3) to evaluate the effect of oxidative stress on these muscles.Methods: C57BL6 mice were divided in two groups, with one group receiving 3 mg/kg intraperitoneal injections of edaravone (I/R + Ed group) and the other group receiving an identical amount of saline (I/R group) 30 minutes before ischemia. Edaravone (3-methy-1-pheny1-2-pyrazolin-5-one) is a potent and novel synthetic scavenger of free radicals. This drug inhibits both nonenzymatic lipid peroxidation and the lipoxygenase pathway, in addition to having potent antioxidant effects against ischemia reperfusion. The duration of the ischemia was 1.5 hours, with reperfusion at either 24 or 72 hours (3 days). Specimens of gastrocnemius (GC) and anterior tibialis (TA) were removed for histological evaluation and biochemical analysis.Results: This model of I/R injury was highly reproducible in histologic muscle damage. In the histologic damage score, the mean muscle fibers and inflammatory cell infiltration in the I/R + Ed group were significantly less than the corresponding values of observed in the I/R group. Thus, pretreatment with edaravone was observed to have a protective effect on muscle damage after a period of I/R in mice. In addition, the mean muscle injury score in the I/R + Ed group was also significantly less than the I/R group. In the I/R + Ed group, the mean malondialdehyde (MDA) level was lower than in the I/R group and western-blotting revealed that edaravone pretreatment decreased the level of inducible nitric oxide synthase (iNOS) expression.Conclusions: Edaravone was found to have a protective effect against I/R injury by directly inhibiting lipid peroxidation of the myocyte by free radicals in skeletal muscles and may also reduce the secondary edema and inflammatory infiltration incidence of oxidative stress on tissue.
本文 / Department of Orthopaedic Surgery, Graduate School of Medicine Mie University
8
Subjects/Keywords: Ischemia-reperfusion injury; Skeletal muscle; Free radical scavenger; Edaravone; iNOS
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APA (6th Edition):
Hori, K. (2017). Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb. (Thesis). Mie University / 三重大学. Retrieved from http://hdl.handle.net/10076/14575
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hori, Kazuichiro. “Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb.” 2017. Thesis, Mie University / 三重大学. Accessed January 18, 2021.
http://hdl.handle.net/10076/14575.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hori, Kazuichiro. “Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb.” 2017. Web. 18 Jan 2021.
Vancouver:
Hori K. Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb. [Internet] [Thesis]. Mie University / 三重大学; 2017. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10076/14575.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hori K. Protective effect of edaravone for tourniquet-induced ischemia-reperfusion injury on skeletal muscle in murine hindlimb. [Thesis]. Mie University / 三重大学; 2017. Available from: http://hdl.handle.net/10076/14575
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Rochester
22.
Ju, Yawen.
Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis.
Degree: PhD, 2014, University of Rochester
URL: http://hdl.handle.net/1802/28970
► Rheumatoid arthritis (RA) is a chronic inflammatory joint disease in which patients often suffer from arthritic flare. Using longitudinal contrast-enhanced (CE)-MRI to study knee arthritis…
(more)
▼ Rheumatoid arthritis (RA) is a chronic inflammatory
joint disease in which patients often suffer from arthritic flare.
Using longitudinal contrast-enhanced (CE)-MRI to study knee
arthritis in tumor necrosis factor-transgenic (TNF-Tg) mice, we
observed that the popliteal lymph nodes (PLN) firstly “expand” in
size and contrast enhancement, and then suddenly “collapse” during
arthritic flare. Since CE-MRI is too costly for phenotyping and
longitudinal analyses of PLN, our aim was to develop ultrasound
(US) methods that could replace MRI. In our initial study, we
demonstrated a significant correlation between PLN volumes
determined by US vs. MRI. However, since PLN collapse is more
closely associated with lymphatic draining function than volume, we
evaluated CE-US methods to distinguish changes in lymphatic
transport, which was shown as a biomarker of arthritic flare.
Unfortunately, delivery of the contrast agent prior to US
significantly impairs lymphatic function, making it unsuitable for
phenotyping PLNs. Thus, we went on to develop power Doppler (PD) US
methods to phenotype PLN with greater accuracy and cost
effectiveness vs. CE-MRI.
Another important prior observation we
made is that arthritic flare is associated with the loss of
lymphatic pulse. From other models of inflammation, lymphatic pulse
is known to be controlled by endothelial nitric oxide synthase
(eNOS), and inhibited by inducible NOS (iNOS) expressed in Gr-1+
cells. To test the hypothesis that eNOS/iNOS dysregulation is
responsible for the loss of lymphatic pulse during arthritic flare
in TNF-Tg mice, we performed IHC and in vivo pharmacological
intervention studies with selective and non-selective iNOS
inhibitors. The IHC results demonstrated that large numbers of iNOS
expressing Gr-1+ cells exist in collapsed PLN. By evaluating the
lymphatics with NIR-ICG imaging, we observed that the specific iNOS
inhibitor L-NIL increased lymphatic pulse and afferent lymphatic
drainage in TNF-Tg mice. Additionally, the micro-CT results showed
that bone erosions were ameliorated in L-NIL treated TNF-Tg mice
compared with placebo. Collectively, these results suggest a model
that the accumulation of iNOS-expressing Gr-1+ cells accelerates
the onset of flare in the setting of inflammatory arthritis via
inhibition of lymphatic drainage, and identifies this pathway as a
potential target for RA therapy.
Subjects/Keywords: Rheumatoid Arthritis; Popliteal Lymph Node; TNF-Tg; Ultrasound; iNOS; Guanulate Cyclase
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APA (6th Edition):
Ju, Y. (2014). Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/28970
Chicago Manual of Style (16th Edition):
Ju, Yawen. “Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis.” 2014. Doctoral Dissertation, University of Rochester. Accessed January 18, 2021.
http://hdl.handle.net/1802/28970.
MLA Handbook (7th Edition):
Ju, Yawen. “Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis.” 2014. Web. 18 Jan 2021.
Vancouver:
Ju Y. Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis. [Internet] [Doctoral dissertation]. University of Rochester; 2014. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/1802/28970.
Council of Science Editors:
Ju Y. Elucidating the Role of Lymphatics in the Pathogenesis of
Chronic Inflammatory-Erosive Arthritis. [Doctoral Dissertation]. University of Rochester; 2014. Available from: http://hdl.handle.net/1802/28970
University of Oulu
23.
Vakkala née Mustonen, M. (Merja).
Apoptosis in breast lesions.
Degree: 2000, University of Oulu
URL: http://urn.fi/urn:isbn:9514256506
► Abstract In this work the extent of apoptosis was studied in a set of 504 benign and malignant breast lesions to elucidate its role in…
(more)
▼ Abstract
In this work the extent of apoptosis was studied in a set of 504 benign and malignant breast lesions to elucidate its role in breast tumor development and progression. Also the correlation of apoptosis with estrogen and progesterone receptor positivity, cell proliferation and patients' prognosis was studied. The breast lesions were also analyzed immunohistochemically with antibodies to apoptosis regulating proteins bcl-2 and bax, and caspases 3, 6 and 8. In addition, the immunohistochemical expression of NO• synthesizing enzyme iNOS in relation to apoptosis and angiogenesis was studied. Furthermore, the expression of the antioxidative enzyme MnSOD was studied in relation to apoptosis and cell proliferation.
According to the results, the apoptotic index was lowest in benign breast lesions. It was higher in in situ carcinomas, where a gradual increase in the extent of apoptosis from grade I to III in situ carcinoma was seen. The apoptotic index in invasive carcinomas was higher than in in situ carcinomas, and also in invasive carcinomas there was a gradual increase in apoptosis from grade I to III carcinomas. The apoptotic index was highest in recurrent carcinomas.
Strong bcl-2 expression was usually found in benign breast lesions but the immunoreactivity decreased in in situ and invasive carcinomas. There was a significant inverse association between bcl-2 immunoreactivity and the extent of apoptosis. Low bcl-2 immunoreactivity also associated with estrogen- or progesterone receptor negativity. In contrast, bax expression did not show any significant association with apoptosis, hormone receptors or the histologic types of tumors. Strong cytoplasmic caspase 3, 6 and 8 immunoreactivity was found in most carcinomas. It was weaker in in situ carcinomas and only weak immunoreactivity could be seen in benign breast lesions. There was a significant association between the extent of apoptosis and caspase immunoreactivity.
iNOS expression was found in both tumor and stromal cells. iNOS expression in tumor cells was more frequently found in invasive than in in situ carcinomas. Its expression correlated significantly with a high apoptotic index and high vascularization of the lesion. There was significantly less MnSOD immunoreactivity in invasive breast carcinomas compared to in situ carcinomas or benign hyperplasias. MnSOD immunoreactivity did not associate with the extent of apoptosis, but there was a marginal inverse association between cell proliferation and MnSOD expression.
Increased apoptosis was significantly associated with a high cell proliferation, and inversely associated with a positive estrogen status. A high apoptotic index (< 0.50%) was associated with a decreased survival of the patients.
The results of this study show that apoptosis plays a decisive role in the development and progression of breast carcinoma. It is influenced not only by apoptosis regulating proteins, such as bcl-2 and caspases, but also by the estrogen receptor status. Apoptosis was also associated with iNOS positivity,…
Subjects/Keywords: MnSOD; bcl-2; caspases; iNOS
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APA (6th Edition):
Vakkala née Mustonen, M. (. (2000). Apoptosis in breast lesions. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9514256506
Chicago Manual of Style (16th Edition):
Vakkala née Mustonen, M (Merja). “Apoptosis in breast lesions.” 2000. Doctoral Dissertation, University of Oulu. Accessed January 18, 2021.
http://urn.fi/urn:isbn:9514256506.
MLA Handbook (7th Edition):
Vakkala née Mustonen, M (Merja). “Apoptosis in breast lesions.” 2000. Web. 18 Jan 2021.
Vancouver:
Vakkala née Mustonen M(. Apoptosis in breast lesions. [Internet] [Doctoral dissertation]. University of Oulu; 2000. [cited 2021 Jan 18].
Available from: http://urn.fi/urn:isbn:9514256506.
Council of Science Editors:
Vakkala née Mustonen M(. Apoptosis in breast lesions. [Doctoral Dissertation]. University of Oulu; 2000. Available from: http://urn.fi/urn:isbn:9514256506
24.
Tavladaki, Theonymfi.
Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη.
Degree: 2018, University of Crete (UOC); Πανεπιστήμιο Κρήτης
URL: http://hdl.handle.net/10442/hedi/43706
► Despite the fact that sepsis was already known as a serious condition since Hippocrates, the debate about what sepsis represents and how it should be…
(more)
▼ Despite the fact that sepsis was already known as a serious condition since Hippocrates, the debate about what sepsis represents and how it should be delimited continues until today. While the definitions of sepsis, severe sepsis, septic shock and multi-organ failure have remained unchanged for more than 2 decades, a new definition emerged in 2016 called SEPSIS -3. This process was based on the recent knowledge that sepsis has implications in organ function , morphology, cell biology, biochemistry, immunology and circulation that all together constitute the pathobiology of sepsis. The prevailing severity score scale is the Sequential Organ Failure Assessment (SOFA). The higher SOFA scale is associated with higher mortality. Regarding children, there are corresponding definitions. Over the past 2 decades, case mortality has dropped whereas overall mortality increased.The term "systemic inflammatory response syndrome (SIRS)" was introduced to describe the host's hyperflammatory response to pathogen invasion, which was considered the hallmark of sepsis. Later, Bone et al. promoted the idea that the initial inflammatory response is followed by "compensatory anti-inflammatory response syndrome (CARS)", characterized by the induction of various anti-inflammatory mechanisms. In recent years, it has become apparent that the infection contributes both pro-inflammatory and anti-inflammatory mechanisms. Along with cytokines and chemokines, the term lipokines is used in the literature to describe protein molecules involved in inflammation and insulin resistance. Resistin as a proinflammatory lipokine increases in severe inflammation. Adiponectin is reduced in obese and severely affected patients. It is well known that all organisms respond to adverse environmental conditions by producing a set of specific stress-resistance proteins called heat shock proteins (Hsps) or chaperones. With regard to Hsp90, it has been shown that elevated levels of Hsp90 in plasma are associated with worse outcome in critically ill children with septic shock. Many researchers assume that moderate levels of stress, such as sepsis, increase the expression of intracellular HSP and secretion of extracellular HSP. Increased extracellular Hsp72 is associated with poor outcome in septic shock patients. Since HSP72 gene polymorphisms are also correlated with HSP72 production, polymorphisms of a nucleotide (SNPs) may be determinants of the sensitivity of the individual.The dysfunction of organs observed in sepsis appears to be due, at least in part, to mitochondrial dysfunction due to the developing strong oxidative stress and consequent failure of energy production confirmed in animal models of sepsis. Oxidative stress occurs when ROS / RNS production and antioxidant mechanisms are not in equilibrium. Nitrogen Oxide (NO) is produced by three different synthases (NO synthases, NOS). The enzyme, the inducible NOS (iNOS ), is synthesized in response to inflammation and produces large amounts of NO for prolonged periods of time. Different interpretations can be…
Subjects/Keywords: Πρωτεΐνες θερμικής καταπληξίας; Σήψη; Μιτοχόνδριο; Heat shock proteins; Sepsis; iNOS; Mitochondrion
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APA (6th Edition):
Tavladaki, T. (2018). Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη. (Thesis). University of Crete (UOC); Πανεπιστήμιο Κρήτης. Retrieved from http://hdl.handle.net/10442/hedi/43706
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tavladaki, Theonymfi. “Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη.” 2018. Thesis, University of Crete (UOC); Πανεπιστήμιο Κρήτης. Accessed January 18, 2021.
http://hdl.handle.net/10442/hedi/43706.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tavladaki, Theonymfi. “Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη.” 2018. Web. 18 Jan 2021.
Vancouver:
Tavladaki T. Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη. [Internet] [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/10442/hedi/43706.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tavladaki T. Συσχέτιση της κατανάλωσης ενέργειας με τη μιτοχονδριακή λειτουργία και τη διέγερση του iNOS και των πρωτεϊνών στρες Hsp70, Hsp 90 σε ασθενείς ΜΕΘ με σήψη. [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. Available from: http://hdl.handle.net/10442/hedi/43706
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
25.
Dobutović, Branislava D., 1977-.
Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova.
Degree: Biološki fakultet, 2014, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular Endocrinology
U poslednjih nekoliko godina, objavljeni naučni podaci sve više ukazuju na antioksidantne i antiinflamatorne efekte grelina.…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular Endocrinology
U poslednjih nekoliko godina, objavljeni naučni
podaci sve više ukazuju na antioksidantne i antiinflamatorne efekte
grelina. U ovoj doktorskoj disertaciji su izučavani efekti grelina
na ekspresiju enzima jetre: superoksid dismutaze (engl. Superoxide
Dismutase, SOD), katalaze (engl. Catalase, CAT), glutation
peroksidaze (engl. Glutathione peroxidase, GPx) i glutation
reduktaze (engl. Glutathione reductase, GR), kao i ekspresiju
njihovog uzvodnog regulatora, nuklearnog faktora-kB (engl. Nuclear
factor- kappa B, NFκB). Takođe su izučavani efekti grelina na
regulaciju enzima inducibilne azot-monoksid-sintaze (engl.
Inducible nitric oxide synthase, iNOS; NOS2) u jetri pacova. Jedan
od ciljeva istraživanja u okviru ove doktorske disertacije je bio i
utvrđivanje uloge kinaze regulisane ekstraćelijskim signalima
(engl. Extracellular regulated protein kinase, ERK1/2) i protein
kinaze B (engl. Protein kinase B, Akt) u grelinom regulisonoj
aktivnosti i ekspresiji antioksidatnivnih enzima i iNOS u jetri
pacova. Kontrolni, normalno hranjeni mužjaci pacova, Wistar soja
(mase 200-250g) kao i mužjaci pacova koji su bili na dijeti bogatoj
mastima (engl. High fat diet, HF), tretirani su grelinom
(0,3nmol/5μl) intracerebroventrikularnim (ICV) injeciranjem, u
bočne cerebralne komore svaka 24 h, u trajanju od 5 dana. Dva sata
nakon poslednjeg tretmana, životinje su žrtvovane i izolovane su
jetre. Za utvrđivanje nivoa ekspresije proteina: iNOS,
antioksidativnih enzima, fosforilacije Akt na Ser473, ERK 1/2 i
NFκB, subjedinica p50 i p65, primenjena je Western blot metoda.
Dobijeni rezultati pokazuju da dolazi do povećanja ekspresije
proteina za enzime antioksidativne zaštite: Cu/Zn-SOD (p<0,05),
Mn-SOD (p<0,05), CAT (p<0,01), GPx, (p<0,001), kao i GR
(p<0,01) u grupi normalno hranjenih životinja tretiranih
grelinom u poređenju sa kontrolnim životinjama. Grelin značajno
smanjuje ekspresiju iNOS (p<0,001) kao i fosforilaciju NFκB
subjedinica p65 (p<0,001) i p50 (p<0,05) u grupi normalno
hranjenih životinja u poređenju sa kontrolnom grupom pacova. Nivo
fosforilacije Akt na Ser473 značajno je veći u grelinom tretiranoj
grupi nego kod kontrolnih životinja (p<0,01)...
Advisors/Committee Members: Isenović, Esma R. 1962-.
Subjects/Keywords: ghrelin; Akt; ERK1/2; iNOS; antioxidative ensymes;
NFκB; Liver
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Dobutović, Branislava D., 1. (2014). Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dobutović, Branislava D., 1977-. “Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova.” 2014. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dobutović, Branislava D., 1977-. “Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova.” 2014. Web. 18 Jan 2021.
Vancouver:
Dobutović, Branislava D. 1. Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2014. [cited 2021 Jan 18].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dobutović, Branislava D. 1. Efekat grelina na regulaciju antioksidativnih enzima i
inducibilne azot-monoksid-sintaze u jetri pacova. [Thesis]. Univerzitet u Beogradu; 2014. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:7069/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Univerzitet u Beogradu
26.
Stanimirović, Julijana S., 1988-.
Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova.
Degree: Biološki fakultet, 2018, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:18800/bdef:Content/get
► Biologija - Molekularna endokrinologija / Biology - Molecular Endocrinology
Gojaznost i posledično poremećen metabolizam lipida i glukoze u jetri su glavni faktori rizika za razvoj…
(more)
▼ Biologija - Molekularna endokrinologija / Biology -
Molecular Endocrinology
Gojaznost i posledično poremećen metabolizam lipida
i glukoze u jetri su glavni faktori rizika za razvoj bolesti jetre.
Polne razlike u gojaznosti i razvoju rezistencije na insulin (IR) i
nealkoholne bolesti masne jetre (NAFLD) pripisuju se estrogenima,
ali mehanizmi kojima ovi hormoni ostvaruju pomenute efekte nisu
dovoljno istraženi. Pored toga što ostvaruju genomske efekte,
estrogeni aktiviraju i signalne molekule u citoplazmi:
fosfatidilinozitol 3-kinazu (PI3K), protein kinazu B (Akt), Rho
familiju malih GTP vezujućih proteina (Rho), kao i njihove nishodne
efektore, Rho-vezane protein kinaze (ROCK), zatim AMP-aktivirajuće
protein kinaze (AMPK) i kinaze regulisane ekstraćelijskim signalima
(ERK1/2). Dosadašnja istraživanja su pokazala da, pored protektivne
uloge koju inducibilna azot-monoksid-sintaza (iNOS) ima u jetri
tokom različitih procesa (sepsa i ishemija), poremećaj u regulaciji
njene ekspresije i aktivnosti mogu biti uključeni u razvoj IR u
gojaznosti. Jedna od pratećih komplikacija IR je i poremećaj u
regulaciji ekspresije i aktivnosti natrijum-kalijum adenozin
trifosfataze (Na+/K+-ATP-aza), koja učestvuje u realizaciji brojnih
procesa u jetri. Poznato je da estradiol reguliše ekspresiju iNOS i
Na+/K+-ATPaze, ali podaci koji ukazuju na polne razlike u
regulaciji ekspresije i aktivnosti ovih enzima u jetri gojaznih
životinja nedostaju u literaturi. U eksperimentima ove doktorske
disertacije korišćeni su adultne ženke i mužjaci pacova Wistar
soja. Jedna grupa pacova je hranjena standardnom laboratorijskom
hranom (kontrolni pacovi), a druga standardnom laboratorijskom
hranom obogaćenom sa 42% masti (gojazni pacovi). Nakon 10 nedelja
životinje su žrtvovane, izolovane su jetre, a iz pune krvi su
izdvajani serum i plazma. U serumu pacova određivane su
koncentracije: glukoze, insulina, ukupnog holesterola, dok je u
plazmi određivana koncentracija slobodnih masnih kiselina (SMK) i
koncentracija nitrita (NO2-) i nitrata (NO3-), kao krajnjih
produkata NO. U lizatu jetre pacova određivana je koncentracija
glukoze, holesterola i SMK, kao i aktivnost Rho proteina. Aktivnost
Na+/K+-ATPaze je određivana u frakciji plazma membrana ćelija
jetre. Nivo iRNK za iNOS i α1 subjedinicu Na+/K+-ATPaze određivan
je metodom qPCR...
Advisors/Committee Members: Isenović, Esma R., 1962-.
Subjects/Keywords: iNOS; liver; Na+/K+-ATPase; obesity; sex
differences
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Stanimirović, Julijana S., 1. (2018). Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:18800/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Stanimirović, Julijana S., 1988-. “Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova.” 2018. Thesis, Univerzitet u Beogradu. Accessed January 18, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:18800/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Stanimirović, Julijana S., 1988-. “Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova.” 2018. Web. 18 Jan 2021.
Vancouver:
Stanimirović, Julijana S. 1. Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2018. [cited 2021 Jan 18].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18800/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Stanimirović, Julijana S. 1. Polne razlike u regulaciji ekspresije i aktivnosti
inducibilne azot-monoksid-sintaze i natrijum-kalijumove pumpe u
jetri gojaznih pacova. [Thesis]. Univerzitet u Beogradu; 2018. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:18800/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Michael, Alyona.
In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain.
Degree: 2018, Iowa State University
URL: https://lib.dr.iastate.edu/etd/16760
► Transmissible spongiform encephalopathies constitute a group of mammalian neurodegenerative protein misfolding disorders, characterized by neuronal loss and gliosis in response to accumulation of an abnormal…
(more)
▼ Transmissible spongiform encephalopathies constitute a group of mammalian neurodegenerative protein misfolding disorders, characterized by neuronal loss and gliosis in response to accumulation of an abnormal conformer (PrPsc) of the native cellular prion protein (PrPc). The nature of the local inflammatory response and the potential contributions of microgliosis and astrocytosis to the progression of neuropathology have not been fully resolved. Shifts in microglial and astrocytic immunophenotypes have been demonstrated in other human neurodegenerative protein misfolding diseases. Similarly, we anticipated a fluid glial activation profile, characterized by transitions in phenotype markers and immunoproteasome induction, over the course of prion infection. This dissertation sought to characterize the neuroinflammatory response to prion infection using a murine intracranial infection model.
Successive chromogenic immunolabeling and in-situ hybridization were employed in analyzing expression patterns of glial activation markers and a proteasomal subtype (PSMB10) over the timecourse of infection in a murine scrapie model. Our model successfully recapitulated classical patterns of TSE-associated neuropathology and demonstrated a precocious microglial response, relative to other studies. We also identified an upregulation of the proinflammatory enzyme iNOS in glial populations at late stages of disease incubation. Colocalization analysis of glial cytoplasmic and activation markers allowed us to resolve an astrocyte-associated increase in Arg1 expression in clinical disease, despite lack of significant changes in global Arg1 expression. Although quantification of immunoproteasome subunit PSMB10 expression failed to yield significant temporospatial trends, this analysis characterized baseline expression patterns across 16 brain regions. Combined, these findings constitute a comprehensive in-situ evaluation of glial activation and present techniques novel to prion research.
Subjects/Keywords: Arg1; iNOS; Mouse; Prion; Scrapie; Neuroscience and Neurobiology; Pathology
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Michael, A. (2018). In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16760
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Michael, Alyona. “In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain.” 2018. Thesis, Iowa State University. Accessed January 18, 2021.
https://lib.dr.iastate.edu/etd/16760.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Michael, Alyona. “In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain.” 2018. Web. 18 Jan 2021.
Vancouver:
Michael A. In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain. [Internet] [Thesis]. Iowa State University; 2018. [cited 2021 Jan 18].
Available from: https://lib.dr.iastate.edu/etd/16760.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Michael A. In-situ temporospatial characterization of the neuroinflammatory response to prion infection in the murine brain. [Thesis]. Iowa State University; 2018. Available from: https://lib.dr.iastate.edu/etd/16760
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Oklahoma State University
28.
Saffarian Tousi, Neda.
Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells.
Degree: Department of Biochemistry and Molecular Biology, 2011, Oklahoma State University
URL: http://hdl.handle.net/11244/6674
► The findings from the current study concluded a cytokine-dependent regulation of astroglial CXCL10 and iNOS expression by alpha-synuclein and neuromelanin. Alpha-synuclein induced the expression of…
(more)
▼ The findings from the current study concluded a cytokine-dependent regulation of astroglial CXCL10 and
iNOS expression by alpha-synuclein and neuromelanin. Alpha-synuclein induced the expression of CXCL10 in both IL-1beta- and TNFalpha-stimulated astroglial cells, however, this enhancement by alpha-synuclein was not demonstrated for the mRNA. Studies revealed the ability of alpha-synuclein to mediate an increase in mRNA stability, which may be, in part, the mechanism responsible for the increase in CXCL10 protein expression. The NF-kappaB signaling pathway was not activated by alpha-synuclein under similar treatments that induced CXCL10 expression yet was activated in thevpresence of other alpha-synuclein concentrations, suggesting the expression of other inflammatory mediators besides CXCL10 may be modulated by alpha-synuclein. Alpha-synuclein also increased astroglial
iNOS expression, further supporting the inflammation-mediated effects by alpha-synuclein.Investigation of role of NM on astroglial chemokine expression revealed a down-regulation of CXCL10 protein expression with a reduction in NF-kappaB activation, suggesting a possible mechanism responsible for decrease in CXCL10 protein. NM enhanced cytokine-induced
iNOS expression in the presence of astrocytes; however, further studies need to be conducted to understand the mechanism.Overall, the findings provide an initial understanding of the effects of PD-associated molecules and inflammation on human brain cells. The regulation of astroglial-derived inflammatory mediators by extracellular alpha-synuclein and neuromelanin may play a role in PD-associated neuroinflammation. The observations provide novelinformation that may be useful in identifying molecular targets for therapeutic intervention in PD.
Advisors/Committee Members: Davis, Randall L. (advisor), Stevens, Craig W. (committee member), Miller, Kenneth E. (committee member), Kaul, Rashmi (committee member).
Subjects/Keywords: alpha-synuclein; cxcl10; inos; neuromelanin; nf-kappab; parkinson's disease
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APA ·
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APA (6th Edition):
Saffarian Tousi, N. (2011). Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/6674
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Saffarian Tousi, Neda. “Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells.” 2011. Thesis, Oklahoma State University. Accessed January 18, 2021.
http://hdl.handle.net/11244/6674.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Saffarian Tousi, Neda. “Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells.” 2011. Web. 18 Jan 2021.
Vancouver:
Saffarian Tousi N. Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells. [Internet] [Thesis]. Oklahoma State University; 2011. [cited 2021 Jan 18].
Available from: http://hdl.handle.net/11244/6674.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Saffarian Tousi N. Neuromelanin and Alpha-synuclein Modulation of Inflammatory Signaling in Human Astroglial Cells. [Thesis]. Oklahoma State University; 2011. Available from: http://hdl.handle.net/11244/6674
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Michigan Technological University
29.
Hopkins, Sean.
DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION.
Degree: PhD, Department of Biomedical Engineering, 2015, Michigan Technological University
URL: https://digitalcommons.mtu.edu/etdr/22
► Implanted medical devices undergo complications the longer they remain in contact with tissue or blood. This rejection of foreign materials by our body is…
(more)
▼ Implanted medical devices undergo complications the longer they remain in contact with tissue or blood. This rejection of foreign materials by our body is one of the largest reasons innovations in biomedical sensors and implanted technology are being held back. One means to hold off this unwanted response is through the utilization of nitric oxide (NO) releasing materials. Two unique NO releasing polymeric materials were synthesized and characterized before being implanted subcutaneously. Both NO releasing materials described used S-nitrosothiol (RSNO) chemistry as the main mechanism for NO release. The first material described covalently links an RSNO to the backbone of PVC while the second material has RSNOs covalently attached to a hyperbranched polyamidoamine (HPAMAM) molecule, which is then blended within a polymer matrix. A high reservoir of NO was observed in the NO releasing HPAMAM when compared to other NO releasing polymers.
The two materials (SNAP-PVC, SNAP-HPAMAM blended in PVC) were implanted subcutaneously and were tested versus control polymers that did not release NO; materials were explanted after 1 and 15 days and histological characterization was completed. The inflammatory response was then observed through histological analysis and NO demonstrated anti-inflammatory properties, specifically by observing the presence of cells marked with CD11b, CD163, and
iNOS. Fibrosis was also a major inflammatory response carefully observed. NO releasing implants showed a much more resolved state of inflammation and wound healing while the controls demonstrated signs of chronic inflammation and increased number of pro-inflammatory cells. The long lasting SNAP-HPAMAM PVC NO releasing materials showed a large reduction in chronic inflammatory macrophages marked with
iNOS with a slight upregulation in anti-inflammatory macrophages after 15 days of implantation. Compared to control PVC implants, a significant reduction in fibrosis was observed as the encapsulation thickness was 120.28±36.1 µm while SNAP-PVC was 74.20±29.9 µm and SNAP-HPAMAM was 38.68±21.0 µm. A trend was seen in the reduction of CD11b
+ cells with an increase in NO from the implants, along with an increasing trend of CD163
+ cells. The presence of chronic inflammatory
iNOS cells was also greatly reduced with the increase of NO to the surrounding subcutaneous tissue.
Advisors/Committee Members: Megan Frost.
Subjects/Keywords: nitric oxide; hyperbranched polymer; polyvinyl chloride; macrophage; inflammation; iNOS; Biomaterials
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hopkins, S. (2015). DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION. (Doctoral Dissertation). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/22
Chicago Manual of Style (16th Edition):
Hopkins, Sean. “DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION.” 2015. Doctoral Dissertation, Michigan Technological University. Accessed January 18, 2021.
https://digitalcommons.mtu.edu/etdr/22.
MLA Handbook (7th Edition):
Hopkins, Sean. “DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION.” 2015. Web. 18 Jan 2021.
Vancouver:
Hopkins S. DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION. [Internet] [Doctoral dissertation]. Michigan Technological University; 2015. [cited 2021 Jan 18].
Available from: https://digitalcommons.mtu.edu/etdr/22.
Council of Science Editors:
Hopkins S. DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION. [Doctoral Dissertation]. Michigan Technological University; 2015. Available from: https://digitalcommons.mtu.edu/etdr/22
Washington University in St. Louis
30.
Mandell, Michael.
Leishmania persistence and host cell interactions.
Degree: PhD, Biology and Biomedical Sciences: Molecular Microbiology and Microbial Pathogenesis, 2011, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/etd/226
► Leishmania parasites are the causative agent of leishmaniasis, a neglected tropical disease. An important aspect of Leishmania biology is asymptomatic parasite persistence, which typically occurs…
(more)
▼ Leishmania parasites are the causative agent of leishmaniasis, a neglected tropical disease. An important aspect of Leishmania biology is asymptomatic parasite persistence, which typically occurs after clinical cure. Persistent parasites remain enigmatic despite their importance as reservoirs for transmission, having roles in maintaining protective immunity, and posing the risk of reactivation. I developed methods for assessing parasite replication by BrdU labeling and showed that persistently infected mice harbor two sub-populations of L. major, one labeling similarly to acute-phase parasites, with the other showing much less labeling. That persistent parasite replication occurs without a commensurate increase in parasite number implies parasite killing. Continual parasite replication and destruction within antigen presenting cells provides an attractive model explaining the role of persistent parasites in maintaining immunity, namely through constant presentation of antigens derived from dead parasites and subsequent immune boost. While many of the persistent parasites are within host cells expressing high levels of
iNOS, there is no apparent correlation between this and the parasite's survival/replication status. Attenuated lpg2- L. major, a proposed model of parasite persistence, resemble WT persistent parasites for most parameters tested. However, more lpg2- parasites are associated with host cells expressing elevated levels of arginase 1, which further studies implicate as a negative correlate of immunity. While persistent parasites immunize their hosts against pathology from subsequent infection, experiments using marked parasites showed that persistently infected mice could be super-infected. This has implications for the generation of parasite phenotypic diversity, as genetically distinct parasites could be simultaneously transmitted to sand flies, the site of parasite sexual recombination. In addition to my studies of Leishmania persistence, I also identified markers that differentiate amastigote- from metacyclic-stage parasites, and used them to assay parasite differentiation within different host cell types in vitro. Although the markers were induced in the same sequence in all host cell types, the parasites in bone marrow-derived macrophages and dendritic cells were slower to lose LPG expression and resume replication. These data show that invading L. major can retain virulence factors, potentially playing a role in situations where parasites are transferred from one host cell to another.
Advisors/Committee Members: Stephen Beverley.
Subjects/Keywords: Microbiology; concomitant immunity, iNOS, latent, Leishmania, persistence, vaccination
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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Manager
APA (6th Edition):
Mandell, M. (2011). Leishmania persistence and host cell interactions. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/226
Chicago Manual of Style (16th Edition):
Mandell, Michael. “Leishmania persistence and host cell interactions.” 2011. Doctoral Dissertation, Washington University in St. Louis. Accessed January 18, 2021.
https://openscholarship.wustl.edu/etd/226.
MLA Handbook (7th Edition):
Mandell, Michael. “Leishmania persistence and host cell interactions.” 2011. Web. 18 Jan 2021.
Vancouver:
Mandell M. Leishmania persistence and host cell interactions. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2011. [cited 2021 Jan 18].
Available from: https://openscholarship.wustl.edu/etd/226.
Council of Science Editors:
Mandell M. Leishmania persistence and host cell interactions. [Doctoral Dissertation]. Washington University in St. Louis; 2011. Available from: https://openscholarship.wustl.edu/etd/226
◁ [1] [2] [3] [4] ▶
. 
Open Access Theses and Dissertations
