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Freie Universität Berlin

1. Mateika, Silke. Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice.

Degree: 2008, Freie Universität Berlin

Extensive basic knowledge on inbred mouse strains and specific mouse mutants is essential to use mouse models in biomedical science. The specific genetic background of the different inbred mouse strains is known to be responsible for the considerable variations in the reaction of mice to infections, such as Yersinia infections. This observation indicated that differences in intensity and characteristics of the immune response between mouse strains should also exist on histological level. On the one hand, this study aimed at adding to the data on basic knowledge of inbred mouse strains. On the other hand, histological evidence was to be provided of qualitative and quantitative differences in the examined inbred mouse strains reaction to an infection with Y. enterocolitica. Subsequently, an order of susceptibility and, for the first time, also of the immunity of the studied mouse strains was established. In the present study, four inbred wild-type mouse strains (C57BL/6OlaHsd, BALB/cOlaHsd, C3H/HeNHsd and 129P2/OlaHsd) were orally infected with a low- virulence strain of Y. enterocolitica. A systematic histopathomorphological analysis of the aboral small intestine was performed three and nine days p.i. The main focus was the characterization of the type and degree of inflammatory reactive patterns and the number and distribution of Yersinia colonies. Specifically, the influences of genetic backgrounds, gender and time point after infection were assessed. Yersinia colonies and the changes in the tissue associated with the Yersinia infection were detected in all mouse strains in the mucosa as well as in the PP. Qualitative and quantitative differences in the inflammatory response, the colonies distribution and the course of colony number were recorded between the different inbred mouse strains. For the first time, this study could demonstrate histopathologically that the inbred mouse strains studied differed in the character of the inflammatory changes. In the present study, C57BL/6OlaHsd was classified as intermediate susceptible because of its high number of colonies and as strain with the best immunity. The bacteria were eliminated by a strong, effective immune response during infection. Macrophages could be detected very early. Furthermore, a partial recovery by granulation tissue and perivascular plasma cell infiltrates was observed histologically. The strain BALB/cOlaHsd showed high resistance to the initial infection and the least effective immunity of the present study. Three days p.i., there were relatively low colony numbers but the strain did not show any maturation of the inflammatory response. The number of bacterial colonies and altered PP even increased during the infection. Similar purulent changes of the tissue were observed at both time points of examination. Organisation of the alterations hardly occured, and the plasma cell infiltration into the PP did not increase significantly. The animals of this strain were thus not able to confine the infection. The strain C3H/HeNHsd was classified as the most… Advisors/Committee Members: n (gender), Univ.-Prof. Dr. A.D. Gruber, PhD. (firstReferee), Univ.-Prof. Dr. L.H. Wieler (furtherReferee), Univ.-Prof. Dr. M.F.G. Schmidt (furtherReferee).

Subjects/Keywords: Mice; Yersinia enterocolitica; intestines; inflammation; bacterial disease; immunity; macrophages; genetic resistance; disease resistance; histopathology; histological staining; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

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APA (6th Edition):

Mateika, S. (2008). Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-5797

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mateika, Silke. “Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice.” 2008. Thesis, Freie Universität Berlin. Accessed September 22, 2020. http://dx.doi.org/10.17169/refubium-5797.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mateika, Silke. “Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice.” 2008. Web. 22 Sep 2020.

Vancouver:

Mateika S. Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2020 Sep 22]. Available from: http://dx.doi.org/10.17169/refubium-5797.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mateika S. Influence of the genetic background on the jejunal pathology following oral infection with Yersinia enterocolitica in mice. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-5797

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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