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You searched for subject:(heteroarthrocarpy). Showing records 1 – 2 of 2 total matches.

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University of Alberta

1. Brock, Kelsey C. Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae.

Degree: MS, Department of Biological Sciences, 2014, University of Alberta

Flowers of Brassicaceae are remarkably similar across all species, whereas their fruits vary in almost all conceivable traits, particularly in the tribe Brassiceae. In contrast, Brassicaceae’s sister family, Cleomaceae, exhibits substantial variation in flowers but are more uniform in their fruits. These diversifications represent either variation in pollen transfer or seed dispersal, which are important reproductive traits that likely affect survival. The history of both families involves four shared polyploidy events as well as each independently experiencing separate additional polyploid events. Thus, these families offer an excellent opportunity to investigate whether additional genetic materials from polyploid events are correlated with the evolution of novel flower and fruit morphologies. Based on knowledge from model plants, FRUITFULL (FUL), a fruit development gene important for dehiscence (fruit opening), and TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL FACTOR 1 (TCP1), a gene known to affect floral symmetry, were chosen as candidate genes to examine the evolutionary history and retention of gene duplicates alongside morphological novelty in Brassicaceae and Cleomaceae. A gene phylogeny of FUL was generated to determine if fruit diversity in the tribe Brassiceae (Brassicaceae) could be correlated with FUL copy number, structure, or evolutionary history. Similarly, TCP1 was assessed to identify differences in gene evolutionary history between the florally diverse Cleomaceae and the florally uniform Brassicaceae. Both FUL and TCP1 were found to exhibit complex evolutionary histories, with multiple copies of these genes found in both taxa with and without morphological novelty. However, evaluation of which copies were retained and the rates of selection acting on these genes suggest their involvement in generating morphological diversity of reproductive structures. This thesis presents a strong correlative framework to direct future hypothesis testing using gene expression and functional approaches to further unravel the genetic changes underlying flower and fruit diversification.

Subjects/Keywords: heteroarthrocarpy; fruit evolution; gene phylogeny; intron length; floral symmetry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Brock, K. C. (2014). Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/rn3011895

Chicago Manual of Style (16th Edition):

Brock, Kelsey C. “Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae.” 2014. Masters Thesis, University of Alberta. Accessed October 14, 2019. https://era.library.ualberta.ca/files/rn3011895.

MLA Handbook (7th Edition):

Brock, Kelsey C. “Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae.” 2014. Web. 14 Oct 2019.

Vancouver:

Brock KC. Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2019 Oct 14]. Available from: https://era.library.ualberta.ca/files/rn3011895.

Council of Science Editors:

Brock KC. Tracking the Evolutionary History of Development Genes: Implications for the Diversification of Fruits and Flowers in the Brassicaceae and Cleomaceae. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/rn3011895


University of Alberta

2. Mankowski, Peter J. A functional approach to profiling candidate genes in non model Brassicales.

Degree: MS, Department of Biological Sciences, 2013, University of Alberta

Obtaining functional data is an essential component in understanding mechanisms underlying morphological variation. Virus-induced gene silencing (VIGS) is a reverse genetic technique for identifying the function of target loci through viral mediated transcript knockdown. Here we introduce this technique to two plant systems Cleome violacea (Cleomaceae) and Erucaria erucarioides (Brassicaceae) chosen for their morphological traits and phylogenetic position. In both species, a construct with a heterologous sequence and a construct with an endogenous sequence of the visual marker PHYTOENE DESATURASE (PDS) were incorporated into separate viral constructs for downregulation. Downregulation using a heterologous sequence produced a comparable fold change in transcript abundance to the endogenous sequence in both species however C. violacea was more susceptible to this process. Additional targets FRUITFULL (FUL) and a TCP1 homologue were targeted for knockdown within C. violacea to extend the application of this technique. A protocol for VIGS application is now available to both species for optimization and future functional analysis.

Subjects/Keywords: Brassicales; virus induced gene silencing; evolutionary development; heteroarthrocarpy; Brassiceae; floral symmetry; VIGS; Cleomaceae; monosymmetry; Cleome

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mankowski, P. J. (2013). A functional approach to profiling candidate genes in non model Brassicales. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/ff3655460

Chicago Manual of Style (16th Edition):

Mankowski, Peter J. “A functional approach to profiling candidate genes in non model Brassicales.” 2013. Masters Thesis, University of Alberta. Accessed October 14, 2019. https://era.library.ualberta.ca/files/ff3655460.

MLA Handbook (7th Edition):

Mankowski, Peter J. “A functional approach to profiling candidate genes in non model Brassicales.” 2013. Web. 14 Oct 2019.

Vancouver:

Mankowski PJ. A functional approach to profiling candidate genes in non model Brassicales. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2019 Oct 14]. Available from: https://era.library.ualberta.ca/files/ff3655460.

Council of Science Editors:

Mankowski PJ. A functional approach to profiling candidate genes in non model Brassicales. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/ff3655460

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