subject:(hepatoma cells)

Hong Kong University of Science and Technology

1. Luo, Dan. Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines.

Degree: 1998, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-1675 ; https://doi.org/10.14711/thesis-b612494 ; http://repository.ust.hk/ir/bitstream/1783.1-1675/1/th_redirect.html

► Human hepatoma is a vicious cancer, widespread in Asia, which does not respond to traditional therapies. Recent researches have indicated that through manipulating the regulators… (more)

▼ Human hepatoma is a vicious cancer, widespread in Asia, which does not respond to traditional therapies. Recent researches have indicated that through manipulating the regulators of apoptosis such as the members of the Bcl-2 family, the chemosensitivity of cancer cells could be changed. The objective of this research is through studying the role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines, to explore whether manipulation of Bcl-2 family members can make human hepatoma cells more sensitive to chemotherapeutic agents. In the present study, we used the chemotherapeutic agents taxol and doxorubicin to induce apoptosis in human hepatocellular carcinoma cell line QGY-7703 and hepatoblastoma cell line HepG2. Investigation of the expression profile of Bcl-2 family proteins in both cell lines show that different expression profiles exist between QGY-7703 and HepG2 cells, e.g. QGY-7703 is Bcl-2, Bax, Bcl-XL, Bak and Bad positive, while HepG2 is Bcl-XL, Bak and Bad positive, but Bcl-2 and Bax negative. Expression patterns of Bcl-2 farnily proteins are also different during the apoptotic process triggered by taxol and doxorubicin in these two cell lines, e.g. taxol causes phosphorylation of Bcl-2 protein in QGY-7703 cells; both taxol and doxorubicin cause down-regulation of Bad protein in HepG2, but not in QGY-7703 cells. Our observations in gene transfection experiments indicate that Bcl-2 protein levels are directly proportional to the resistance of QGY-7703 cells to chemotherapeutic agents taxol and doxorubicin. However, the sensitivity to taxol and doxorubicin of HepG2 cells, which are Bcl-2 and Bax negative, is independent of Bcl-2 levels. Bcl-XL gene transfection experiment in HepG2 cells indicates that lower Bcl-XL levels cause HepG2 cells to be more sensitive to taxol and doxorubicin. These fmdings suggest that among the Bcl-2 family members, Bcl-2 and Bax may be the critical proteins in the regulation of apoptosis in QGY-7703 cells, while Bcl-XL and Bak may be the major regulators of apoptosis in HepG2 cells. The observation obtained from M-2 gene transfection experiment performed in HepG2 cells supports the previous notion that without Bax, Bcl-2 loses its apoptosis-inhibiting function. The above observations also suggest that manipulation of Bcl-2 or Bcl-XL may be an effective approach to increase the chemosensitivity of human hepatoma cells, thereby improving the efficiency of hepatoma therapy. The present in vitro study provides the rationale for further exploration in vivo to increase chemosensitivity of human hepatoma through manipulation of Bcl-2 family members.

Subjects/Keywords: Hepatoma ; Apoptosis ; B cells

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APA (6^th Edition):

Luo, D. (1998). Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-1675 ; https://doi.org/10.14711/thesis-b612494 ; http://repository.ust.hk/ir/bitstream/1783.1-1675/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Luo, Dan. “Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines.” 1998. Thesis, Hong Kong University of Science and Technology. Accessed March 08, 2021. http://repository.ust.hk/ir/Record/1783.1-1675 ; https://doi.org/10.14711/thesis-b612494 ; http://repository.ust.hk/ir/bitstream/1783.1-1675/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Luo, Dan. “Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines.” 1998. Web. 08 Mar 2021.

Vancouver:

Luo D. Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines. [Internet] [Thesis]. Hong Kong University of Science and Technology; 1998. [cited 2021 Mar 08]. Available from: http://repository.ust.hk/ir/Record/1783.1-1675 ; https://doi.org/10.14711/thesis-b612494 ; http://repository.ust.hk/ir/bitstream/1783.1-1675/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luo D. Role of Bcl-2 family proteins in apoptosis of human hepatoma cell lines. [Thesis]. Hong Kong University of Science and Technology; 1998. Available from: http://repository.ust.hk/ir/Record/1783.1-1675 ; https://doi.org/10.14711/thesis-b612494 ; http://repository.ust.hk/ir/bitstream/1783.1-1675/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

2. Wu, Chia-Ling. The effect of Dlk overexpression on the tumorigenicity of hepatoma cells.

Degree: Master, Biological Sciences, 2004, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904104-203607

► Dlk is a transmembrane protein that possesses six epidermal growth factor-like sequences at the extracellular domain, a single transmembrane domain and an intracellular tail. The… (more)

▼ Dlk is a transmembrane protein that possesses six epidermal growth factor-like sequences at the extracellular domain, a single transmembrane domain and an intracellular tail. The extracellular EFG-like region of Dlk can be released by action of an unknown protease that cuts the extracellular region near the cell membrane. Dlk belongs to the EGF-like homeotic protein family and has received many names: pG2, FA-1, Pref-1, SCP-1, ZOG and Dlk. All the proteins are identical or polymorphic products of a single gene. Dlk has been involved in several differentiation processes, such as adipogenesis, hematopoiesis and neuroendocrine differentiation. Dlk is also known as the preadipocyte factor-1 (Pref-1), is highly expressed in preadipocytes but is completely abolished in adipocytes. Pref-1 may function in the maintenance of the preadipocyte state and is a negative regulator of adipocyte differentiation. Dlk is expressed in tumors with neuroendocrine features, such as human neuroblastoma, rat pheochromocytoma, and a subset of Small Cell Lung Cancer (SCLC) cell lines. The Dlk expression is probably associated with some differentiation stages because the most undifferentiated cells were lacking expression of Dlk. The finding suggests that Dlk plays an important role in differentiation and tumorigenesis of several cell types. The study was designed to examine the influence of dlk overexpression on tumorigenicity of hepatoma cells. We constructed the mammalian expression vectors for full-length dlk, dlk extracellular domain, which were transfected into SK-Hep-1 cells for generation of stable clones. The transgene expressions in selected stable clones were verified by QRT-PCR and western blot analysis. Our results indicated that overexpression of extracellular domain significantly promoted the viability of SK-Hep1 cells during serum deprivation. In SCID mice, injection of full-length dlk clones led to increased tumor growth compared with the control groups. However, the migration ability was reduced in Dlk stable clones. In summary, these results suggested full-length Dlk promoted the tumor growth but reduced the migration ability of SK-Hep1 cells. Advisors/Committee Members: Cho Chung-Lung (chair), Tai Ming-Hong (committee member), Cheng Jiin-Tsuey (chair), Chuang Jiin-Haur (committee member).

Subjects/Keywords: overexpression; tumorigenicity; hepatoma cells; Dlk protein

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APA (6^th Edition):

Wu, C. (2004). The effect of Dlk overexpression on the tumorigenicity of hepatoma cells. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904104-203607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Chia-Ling. “The effect of Dlk overexpression on the tumorigenicity of hepatoma cells.” 2004. Thesis, NSYSU. Accessed March 08, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904104-203607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Chia-Ling. “The effect of Dlk overexpression on the tumorigenicity of hepatoma cells.” 2004. Web. 08 Mar 2021.

Vancouver:

Wu C. The effect of Dlk overexpression on the tumorigenicity of hepatoma cells. [Internet] [Thesis]. NSYSU; 2004. [cited 2021 Mar 08]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904104-203607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu C. The effect of Dlk overexpression on the tumorigenicity of hepatoma cells. [Thesis]. NSYSU; 2004. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0904104-203607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vytautas Magnus University

3. Sukackaitė, Joana. Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro.

Degree: Master, Medicine, 2008, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080628_091407-13657 ;

►

Didėjat vėžinių susirgimų skaičiui nuolatos ieškoma naujų vaistų, kurie pasižymėtų gerais farmakokinetiniais ir farmakodinaminiais parametrais ir jų veikimas būtų selektyvus. Antioksidantams-antikancerogenams priskiriami kai kurie vitaminai.… (more)

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Didėjat vėžinių susirgimų skaičiui nuolatos ieškoma naujų vaistų, kurie pasižymėtų gerais farmakokinetiniais ir farmakodinaminiais parametrais ir jų veikimas būtų selektyvus. Antioksidantams-antikancerogenams priskiriami kai kurie vitaminai. Vitaminų sukeliami poveikiai navikinėse ląstelėse priklauso nuo navikinių ląstelių tipo, vitaminų dozės. Vitaminų inicijuojama ląstelių žūtis ir jų sunaikinimo keliai gali būti įvairūs: nekrozė, apoptozė, autofagija, autošizė, mitozės sutrikimai ląstelėse. Šiame darbe aprašoma vitaminų C ir K3 citotoksis poveikis ir sinergistinis jų veikimas navikinėse ląstelėse – pelių hepatomos MH-22A, gliomos C6 ir nenavikinėse – Kinijos žiurkėno kiaušidžių ląstelėse. Didėjant l��stelių inkubacijos laikui ir vitaminų koncentracijai, ląstelės pasidaro jautresnės vitaminų poveikiui ir sumažėja gyvybingų ląstelių skaičius. Kombinuojant C ir K3 vitaminus kartu su tam tikromis jų koncentracijomis, sustiprėja vitaminų tarpusavio veikimas. Todėl sumažėja vitaminų koncentracijos reikalingos visiškam navikinių ląstelių nužydymui.

The amount of cancer diseases are growing, because all the time seaching for new chemotherapeutic agents of a cure and point to the need for new therapeutic options with favorable pharmacokinetics and pharmacodynamic parameters as well as selective mechanism of action. It was found that some vitamins are like antioxidants-anticarcinogens. Effect of vitamins dipends on cancer cells line and ratio of vitamins. Vitamins can induce cancer cells death on a different ways like necrosis, apoptosis, autophagy, autoschize and mitotic catastrophe. At this work wrote about vitamins C and K3 cytotoxity and their synergistic apoptosis-inducing actions for cancer cells: mouse hepatoma MH-22A, rat glioma C6 and not for cancer cells - Chinese hamster ovarian cells. If the cells are incubating with vitamins for a long time, they become more sensitive for vitamins and decrease their vitality. Co-adminnistration of vitamin C and vitamin K3 combination to a variety of cancer cell lines resulted in tumor specific antitumor activity at lower doses than when either vitamin was administrated alone.

Advisors/Committee Members: Saulis, Gintautas (Master’s thesis supervisor), Batiuškaitė, Danutė (Master’s thesis reviewer).

Subjects/Keywords: Askorbo rūgštis; Menadionas; Pelės hepatoma; Kinijos žiurkėno kiaušidžių ląstelės; Žiurkės glioma; Ascorbic acid; Menadion; Mouse hepatoma; Chinese hamster ovarian cells; Rat glioma

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APA (6^th Edition):

Sukackaitė, Joana. (2008). Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro. (Masters Thesis). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080628_091407-13657 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Sukackaitė, Joana. “Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro.” 2008. Masters Thesis, Vytautas Magnus University. Accessed March 08, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080628_091407-13657 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Sukackaitė, Joana. “Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro.” 2008. Web. 08 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Sukackaitė, Joana. Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro. [Internet] [Masters thesis]. Vytautas Magnus University; 2008. [cited 2021 Mar 08]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080628_091407-13657 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Sukackaitė, Joana. Vitaminų C ir K3 sinergistinis citotoksinis poveikis ląstelėms in vitro. [Masters Thesis]. Vytautas Magnus University; 2008. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080628_091407-13657 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

4. 김, 지연. Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes.

Degree: 2007, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/1191 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002079

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PURPOSE: Hepatocellular carcinoma (HCC) is a primary malignancy (cancer) of the liver. HCC has been known as androgen-dependent tumor with incidence of five times higher… (more)

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PURPOSE: Hepatocellular carcinoma (HCC) is a primary malignancy (cancer) of the liver. HCC has been known as androgen-dependent tumor with incidence of five times higher and worse prognosis in male than female. Steroid hormones and their receptors play a major role in the development of many types of human and animal cancers. It has been known that androgen and its receptor (AR) are significantly related with hepatocarcinogenesis both in human and animals, however, the level of AR in hepatoma is variable and their exact effects are still poorly explained. Therefore, we investigated the role of androgen receptor (AR) in HCC development. METHOD: To investigate the potential role of AR in hepatoma, Huh7-AR and Huh7-V cell lines were established by transfection of Huh7 hepatocelluar carcinoma (HCC) cells with human androgen receptor (AR) in pCMV5 and its empty vector, respectively. And their in vivo and in vitro tumorigenesis were evaluated in nude mice as well as in cell culture systems. RESULTS: AR overexpressing Huh7 cells, Huh7-AR, and its control, Huh7-V cells, were established. AR expression was measured by RT-PCR and immunoblot analyses. Huh7-AR cells, which were responsive to 5 alpha-dihydrotestosterone (DHT), revealed a decreased tumorigenecity in nude mice in addition to reduced clonogenicity in vitro, as compared with those of Huh7-V cells; Tumor volume and multiplicity of the HCC development in nude mice and invasiveness of the Huh7-AR cells, measured by Matrigel chamber analysis, were significantly reduced than those of Huh7-V cells. Interestingly, when cultured in the presence of DHT, Huh7-AR cells induced cellular senescence phenotypes, such as SA-beta-galactosidase activity, nuclear actin translocation, cytoplasmic p-Erk1/2 sequestration and reduced telomerase activity. When underlying mechanisms were studied in more detail in the cell culture system with or without DHT, TGF-beta1 mRNA expression was increased in the Huh7-AR cells and HCC tumors in the presence of DHT. At the same time, secretion of TGF-beta 1 was significantly higher in the Huh7-AR cells than that in Huh7-V cells. The effect of TGF-beta 1 on the induction of senescence of Huh7-AR cells was studied by infection of dominant negative TGFbeta-RII adenovirus (TGFbeta RII DN-Ad); The reduced growth rate and other cellular senescence phenotypes were significantly recovered after TGFbeta RII DN-Ad infection, compared with the infection of its control viruses. The above data strongly indicate that androgen receptor inhibits progression of HCC formation by Huh7-AR cells through the induction of senescence phenotypes via TGF-beta 1 expression in the presence of DHT.

안드로젠과 안드로젠 수용체(AR)는 사람과 동물에서 간암 발생 과정과 의미있게 관련되어 있음이 알려져 있으나, 간 암 및 주변조직에서 안드로젠 수용체의 발현수준이 다양하여 정확한 효과를 알 수 없었다. 간암 발생과정에 있어 안드로젠과 안드로젠 수용체의 가능한 역할을 알아보기 위해서 사람의 간암 세포주인 Huh7 세포에 pCMV5-hAR과 pCMV5 empty vector를 안정하게 주입하여 G418로 선별하였다. 그 결과 vector만 주입된 clone (이 후로는 Huh7-V라 함)에서는 hAR의 발현이 되지 않았고, hAR이 주입된 clone (이 후로는 Huh7-AR이라 함)에서는 hAR의 발현됨을 확인하였다. Huh7-AR 세포는 5α-dihydrotestosterone…

Advisors/Committee Members: 대학원 의학과, 200124412, 김, 지연.

Subjects/Keywords: Huh7 hepatoma cells; Androgen receptor; Dihydrotestosterone; Cellular senescence; TGF-beta1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

김, �. (2007). Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/1191 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

김, 지연. “Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes.” 2007. Thesis, Ajou University. Accessed March 08, 2021. http://repository.ajou.ac.kr/handle/201003/1191 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

김, 지연. “Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes.” 2007. Web. 08 Mar 2021.

Vancouver:

김 �. Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes. [Internet] [Thesis]. Ajou University; 2007. [cited 2021 Mar 08]. Available from: http://repository.ajou.ac.kr/handle/201003/1191 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002079.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

김 �. Reduction of tumorigenesis in androgen receptor overexpressed Huh7 cells through TGF-β induced cellular senescence phenotypes. [Thesis]. Ajou University; 2007. Available from: http://repository.ajou.ac.kr/handle/201003/1191 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002079

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas Tech University

5. Pennington, Jay B. The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model.

Degree: TTUHSC – Medical Microbiology, 1983, Texas Tech University

URL: http://hdl.handle.net/2346/19628

Subjects/Keywords: Immunoenzyme technique; Hepatoma; Tumors – Immunological aspects; Immune response; T cells

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APA (6^th Edition):

Pennington, J. B. (1983). The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/19628

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pennington, Jay B. “The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model.” 1983. Thesis, Texas Tech University. Accessed March 08, 2021. http://hdl.handle.net/2346/19628.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pennington, Jay B. “The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model.” 1983. Web. 08 Mar 2021.

Vancouver:

Pennington JB. The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model. [Internet] [Thesis]. Texas Tech University; 1983. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2346/19628.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pennington JB. The relationship between immune activity and administration of immobilized peroxidase in a Novikoff hepatoma model. [Thesis]. Texas Tech University; 1983. Available from: http://hdl.handle.net/2346/19628

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kentucky

6. Banerjee, Subhashis. INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL.

Degree: 2010, University of Kentucky

URL: https://uknowledge.uky.edu/gradschool_diss/4

► Cholesterol is an essential component of the cell, but excessive blood levels are a major risk factor for the development of atherosclerotic plaques that can… (more)

▼ Cholesterol is an essential component of the cell, but excessive blood levels are a major risk factor for the development of atherosclerotic plaques that can lead to heart disease and stroke, the foremost cause of premature death in Western societies. Policosanol, a mixture of very long chain alcohols derived from sugarcane, has gained considerable attention among the public as safe and effective means to reduce blood cholesterol levels, a belief based on some early clinical studies. My research investigates one possible mechanism by which policosanol might decrease blood cholesterol levels: the inhibition of cholesterol synthesis in the liver. Previous studies with cultured hepatoma cells have indicated that policosanol suppresses HMG-CoA reductase activity, the regulatory step in cholesterol synthesis, by activation of AMP-kinase, which then inactivates HMG-CoA reductase by phosphorylation. My studies have confirmed this activation of AMP-kinase both in hepatoma cells and in whole animals after intragastric administration of policosanol. The present studies were also undertaken to identify the upstream signaling mechanism by which policosanol activates AMP-kinase. Treatment of rat hepatoma cells with policosanol increased the amount of phosphorylated CaMKK, which can directly activate AMP-kinase, but had only a small effect on LKB1, the principal activator of AMP-kinase. Intragastric administration to mice similarly activated CaMKK, but not LKB1, in the liver. To determine if metabolism of policosanol was necessary for activation of AMP-kinase, siRNA-mediated suppression of fatty aldehyde dehydrogenase, fatty acyl CoA synthase-4, or β-ketothiolase in hepatoma cells prevented the phosphorylation of AMP-kinase and HMG-CoA reductase by policosanol, indicating that metabolism of these very long chain alcohols to fatty acids and subsequent peroxisomal β-oxidation is necessary for the suppression of cholesterol synthesis. As the principal product of fatty acid -oxidation is acetyl-CoA, further studies demonstrated that addition of acetate to cells similarly activated AMP-kinase and inactivated HMG-CoA reductase. This finding argues that the activation of AMP-kinase by policosanol results from the generation of excess acetyl-CoA via peroxisomal metabolism. Finally, although the intestine is a significant source of circulating cholesterol, policosanol was unable to activate AMP-kinase in the small intestine. These findings open new perspectives for the control of cholesterol synthesis by activators of AMP-kinase.

Subjects/Keywords: Cholesterol; AMP kinase; HMG-CoA reductase; Hepatoma cells; Policosanol; Pharmacy and Pharmaceutical Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Banerjee, S. (2010). INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/gradschool_diss/4

Chicago Manual of Style (16^th Edition):

Banerjee, Subhashis. “INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL.” 2010. Doctoral Dissertation, University of Kentucky. Accessed March 08, 2021. https://uknowledge.uky.edu/gradschool_diss/4.

MLA Handbook (7^th Edition):

Banerjee, Subhashis. “INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL.” 2010. Web. 08 Mar 2021.

Vancouver:

Banerjee S. INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL. [Internet] [Doctoral dissertation]. University of Kentucky; 2010. [cited 2021 Mar 08]. Available from: https://uknowledge.uky.edu/gradschool_diss/4.

Council of Science Editors:

Banerjee S. INHIBITION OF CHOLESTEROL SYNTHESIS BY POLICOSANOL. [Doctoral Dissertation]. University of Kentucky; 2010. Available from: https://uknowledge.uky.edu/gradschool_diss/4

7. 坂根, 千春. Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-.

Degree: 博士（栄養学）, 2014, University of Nagasaki / 長崎県立大学

URL: http://hdl.handle.net/10561/1114

Subjects/Keywords: Non-genomic Actions; Diterpenoid Acids; Human Neuroblastoma; Hepatoma Cells; Differentiation Induction; ジテルペノイド酸; ノンゲノミック作用; ヒト神経芽腫; ヒト肝癌細胞; 分化誘導

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APA (6^th Edition):

坂根, �. (2014). Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-. (Thesis). University of Nagasaki / 長崎県立大学. Retrieved from http://hdl.handle.net/10561/1114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

坂根, 千春. “Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-.” 2014. Thesis, University of Nagasaki / 長崎県立大学. Accessed March 08, 2021. http://hdl.handle.net/10561/1114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

坂根, 千春. “Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-.” 2014. Web. 08 Mar 2021.

Vancouver:

坂根 �. Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-. [Internet] [Thesis]. University of Nagasaki / 長崎県立大学; 2014. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10561/1114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

坂根 �. Non-genomic Actions of Diterpenoid Acids : with Special Reference to Differentiation Induction of Human Neuroblastoma and Hepatoma Cells : ジテルペノイド酸のノンゲノミック作用 -特にヒト神経芽腫ならびにヒト肝癌細胞に対する分化誘導について-. [Thesis]. University of Nagasaki / 長崎県立大学; 2014. Available from: http://hdl.handle.net/10561/1114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund

8. Arteaga, Natalia. Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research.

Degree: 2010, University of Lund

URL: https://lup.lub.lu.se/record/1593033 ; https://portal.research.lu.se/ws/files/5802688/1593034.pdf

► A Single-Ion Hit Facility (SIHF) consists of a custom-build facility based in particle accelerators which offers irradiation controlling the number of delivered particles with a… (more)

▼ A Single-Ion Hit Facility (SIHF) consists of a custom-build facility based in particle accelerators which offers irradiation controlling the number of delivered particles with a precise targeting localization. The irradiation spot can be confined down to the nanometre scale allowing the irradiation of subcellular compartments with a single particle. Therefore, these facilities have become a very powerful tool for biological applications specifically to study low-dose radiation effects on living cells. A SIHF has been created at the Lund Nuclear Microprobe (LNM-SIHF) and, in order to make it operational, several tools were fabricated. These tools included the necessary software for cell recognition, custom-designed Petri-type dishes suitable for cell culture and irradiation, and other tools which allow the evaluation of the system. Additionally, the importance of reactive oxygen species (ROS) in bystander cells after non-targeted proton irradiation was investigated on the human hepatoma cell line (HepG2). In-house implemented software, SeACell, provides on-line cell recognition and localization in a short time and high efficiency without the use of cell-staining dyes. The program was developed using IDL 6.2 language, and includes automated and manual targeting selection through a user-friendly interface. In addition, table colour display and filter drop-down menus were added to improve the quality of the input image if required. Custom-designed irradiation dishes permit controlling the cells growth position by confining them through limiting structures on the floor of the dish and therefore, facilitating repeated access to the cell position. The epoxy-based photopolymer SU-8 was patterned by UV lithography technique producing irradiation dishes, with a supporting layer of approximately 5 microns thick where 5 microns height walls were used to form the limiting structure. The entire structure contains 400 squares that can be located by a row letter and column number printed outside the grid. Other tools were manufactured by UV large exposure and the SU-8 photoresist: an artificial cell sample, which offered a semi-realistic scenario to test the system's capability, and a calibration sample used to establish the coordinates of the irradiation point in all the microscopes in which the cells were inspected. Also, two Ni dot arrays were fabricated using electron beam lithography to test the targeting accuracy of the system.

Subjects/Keywords: Subatomic Physics; lithography; UVL; EBL; SeACell; human hepatoma cells (HepG2); reactive oxygen species (ROS); bystander effect; SU-8; cell recognition; cell irradiation; Single-Ion Hit Facility; low-dose; Fysicumarkivet A:2010:Arteaga Marrero

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Arteaga, N. (2010). Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/1593033 ; https://portal.research.lu.se/ws/files/5802688/1593034.pdf

Chicago Manual of Style (16^th Edition):

Arteaga, Natalia. “Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research.” 2010. Doctoral Dissertation, University of Lund. Accessed March 08, 2021. https://lup.lub.lu.se/record/1593033 ; https://portal.research.lu.se/ws/files/5802688/1593034.pdf.

MLA Handbook (7^th Edition):

Arteaga, Natalia. “Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research.” 2010. Web. 08 Mar 2021.

Vancouver:

Arteaga N. Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research. [Internet] [Doctoral dissertation]. University of Lund; 2010. [cited 2021 Mar 08]. Available from: https://lup.lub.lu.se/record/1593033 ; https://portal.research.lu.se/ws/files/5802688/1593034.pdf.

Council of Science Editors:

Arteaga N. Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research. [Doctoral Dissertation]. University of Lund; 2010. Available from: https://lup.lub.lu.se/record/1593033 ; https://portal.research.lu.se/ws/files/5802688/1593034.pdf

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