subject:(hBD2)

1. Καραγκούνης, Παναγιώτης. Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών.

Degree: 2012, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/29781

► Endogenous cationic antimicrobial peptides mediate innate immunity inevery species in which they have been investigated. Cathelicidins anddefensins are the two major families of antimicrobial peptides… (more)

▼ Endogenous cationic antimicrobial peptides mediate innate immunity inevery species in which they have been investigated. Cathelicidins anddefensins are the two major families of antimicrobial peptides in mammals.They are abundant components of phagocytic leukocytes and are released byepithelial cells at mucosal surfaces. In the small intestine, Paneth cells at thebase of the crypts of Lieberkühn secrete α-defensins at high levels inresponse to cholinergic stimulation and when exposed to bacterial antigens.The release of Paneth cell products into the crypt lumen is inferred toprotect mitotically active crypt cells from colonization by potentialpathogens and confers protection from enteric infection. In addition, α-Defensins in Paneth cell secretions may interact with bacteria in theintestinal lumen above the crypt–villus boundary and influence thecomposition of the enteric microbial flora. Human b-defensins were alsodiscovered and characterized during the last decade. In particular, variousaspects of their gene location, expression patterns and the transcriptionfactors involved in their regulation in vivo have been extensively studied.Structural studies of β-defensins provide the molecular basis for a betterunderstanding of their properties, functions and their potential for practicalapplications. The antimicrobial activity of b-defensins, is clearly not theonly function of these peptides. Additional roles of human defensins includethe ability to act as chemokines as well as induce chemokine productionleading to recruitment of leukocytes to the site of infection, the promotionof wound healing and an ability tomodulate adaptive immunity. It appearsthat many of these properties are mediated though direct interaction ofpeptides with the cells of the innate immune response including monocytes,dendritic cells, T cells and epithelial cells. Although studies of theimmunomodulatory properties of these peptides are in their infancy, there isa growing body of evidence suggesting that the immunomodulatoryproperties of these small, naturally occurring molecules might be harnessedfor development as novel therapeutic agents. In our study we focused on157human β-defensin 2 (hBD-2), a 41-amino acid β-defensin. We determinedthe microbicidal activity of synthetic hBD-2 against nosocomial pathogensbelonging to 8 different bacterial and yeast species and exhibiting variousantimicrobial resistance phenotypes. The native disulfide connectivity wasfound essential for the bactericidal activity of hBD-2, while sodium chlorideconcentration was reversely associated with its potency. hBD-2 exhibitedhigh bactericidal activity against Acinetobacter baumannii, Pseudomonasaeruginosa, Enterococcus faecalis, Enterococcus faecium andStaphylococcus aureus clinical strains. Characteristically, A. baumanniistrains that exhibited multidrug-resistant phenotypes were susceptible tolowest concentrations of hBD-2 as compared with sensitive to antibiotics A.baumannii strains. Bactericidal activity of hBD-2 was less pronouncedagainst Escherichia coli, Klebsiella…

Subjects/Keywords: Αμυντίνες; Αντιμικροβιακά πεπτίδια; Νοσοκομειακά στελέχη; Defensins; hBD2; Antimicrobial peptides; Nosocomial strains

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APA (6^th Edition):

Καραγκούνης, . �. (2012). Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/29781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Καραγκούνης, Παναγιώτης. “Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών.” 2012. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 25, 2021. http://hdl.handle.net/10442/hedi/29781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Καραγκούνης, Παναγιώτης. “Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών.” 2012. Web. 25 Jan 2021.

Vancouver:

Καραγκούνης �. Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/10442/hedi/29781.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Καραγκούνης �. Διερεύνηση της δράσης των αντιμικροβιακών πεπτιδίων defensins ( alpha και beta) έναντι διαφόρων παθογόνων μικροοργανισμών. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2012. Available from: http://hdl.handle.net/10442/hedi/29781

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

2. Wang, Bingjie. Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases.

Degree: PhD, 2017, University of Edinburgh

URL: http://hdl.handle.net/1842/28756

► Atopic Dermatitis (AD) is a common chronic relapsing inflammatory skin disease affecting 15 - 20% of children and 2 - 10% of adults worldwide, with… (more)

▼ Atopic Dermatitis (AD) is a common chronic relapsing inflammatory skin disease affecting 15 - 20% of children and 2 - 10% of adults worldwide, with significant morbidity. A hallmark of AD is disruption of the critical barrier function of upper epidermal layers, causatively linked to environmental stimuli, genetics and infections. Another typical feature of AD is skin infections, especially from Staphylococcus aureus (S. aureus), which closely relates with the disease severity. Although not a normal flora, S. aureus is found on 75-100% of AD lesions (< 30% on healthy skin). S. aureus secrete a range of virulence factors, including extracellular toxins and proteases which contribute to disease pathogenesis. S. aureus serine protease A (SspA/V8) is a well-characterised extracellular protease widely expressed among different S. aureus strains. The pathogenic effect of V8 protease has been demonstrated in vivo, damaging murine skin integrity via effects on the stratum corneum (SC), but the targets for this V8-mediated damage remains unclear. The capacity of proteases to induce barrier dysfunction has been proposed as a key driving force in the initiation and exacerbation of AD. Thus, understanding the host factors that maintain barrier function is a priority in developing novel therapeutic approaches. This thesis therefore aimed at detecting host factors which can combat the barrier dysfunction caused by pathogenic proteases, assessing their relevance in vitro and ex vivo and elucidating the underlying mechanisms. Firstly, an in vitro skin barrier integrity model was developed, using both immortalized and primary keratinocytes, to evaluate the barrier damage mediated by pathogenic proteases. The results revealed that S. aureus protease SspA/V8 is the dominant secreted factor (in laboratory and AD clinical strains of S. aureus) inducing barrier integrity impairment. In addition, studies demonstrated that V8 protease itself was sufficient to induce barrier disruption, and this phenotype was not dependent on cell death, but rather on breaking down of cell-cell junctions. Key tight junction proteins including claudin-1 and occludin were found to be degraded by V8 protease. Next, a wide range of host and bacterial factors were investigated to determine whether they could promote protection of keratinocytes against V8 damage. Several factors, including IL-1β, TNF-α, heat-killed Staphylococcus epidermidis (which is the main skin normal flora), were found to induce protection against V8 protease, with IL-1β having the strongest effect. In addition, data indicated that this IL-1β-mediated protection was independent of effects on claudin-1 but occurred via secretion of a transferrable host factor. Induction of keratinocyte expression of the antimicrobial/host defence peptide human beta-defensin 2 (hBD2) was found to be the mechanism underpinning this IL-1β- induced protective effect. Endogenous hBD2 expression was required and sufficient for protection against V8 protease-mediated integrity damage, and exogenous application of hBD2…

Subjects/Keywords: Atopic Dermatitis; Staphylococcus aureus; S. aureus; V8 protease; hBD2; skin barrier dysfunction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, B. (2017). Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/28756

Chicago Manual of Style (16^th Edition):

Wang, Bingjie. “Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases.” 2017. Doctoral Dissertation, University of Edinburgh. Accessed January 25, 2021. http://hdl.handle.net/1842/28756.

MLA Handbook (7^th Edition):

Wang, Bingjie. “Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases.” 2017. Web. 25 Jan 2021.

Vancouver:

Wang B. Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases. [Internet] [Doctoral dissertation]. University of Edinburgh; 2017. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/1842/28756.

Council of Science Editors:

Wang B. Novel function of human beta-defensin 2 : protecting epidermal barrier against pathogenic proteases. [Doctoral Dissertation]. University of Edinburgh; 2017. Available from: http://hdl.handle.net/1842/28756

3. Huang, Shu-Mei. Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing.

Degree: PhD, Biological Sciences, 2015, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823115-001730

► Diabetes mellitus is characterized by elevated plasma glucose and increased rate of skin infection. Altered immune responses have been suggested to contribute to this prevalent… (more)

Subjects/Keywords: hBD3; interleukin-8; keratinocyte; hBD2; high glucose; interleukin-22

…44 圖 3-2 高糖培養下角質細胞 hBD2 的產生狀況 �� ..45 圖 3-3 角質細胞外� IL-1β 時的 hBD2 表現… …48 圖 3-6 AGE-BSA 對角質細胞 hBD2 表現的影響 �� ..…49 圖 3-7 高糖培養及去除糖化終產物對角質細胞 STAT-1 表現的影響… …50 圖 3-8 角質細胞在高糖培養時使用 AG 的 hBD2 表現 �� 51 圖 3-9 角質細胞在正常糖濃度下使用 AG 的表現… …表現，特別是在傷口癒合過程的改變則未被徹底的� �究。hBD1 和 hBD2 對抗的是革蘭氏陰性菌，hBD3 則同時可對抗革蘭氏陽性及陰性菌，包括…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Huang, S. (2015). Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823115-001730

Chicago Manual of Style (16^th Edition):

Huang, Shu-Mei. “Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing.” 2015. Doctoral Dissertation, NSYSU. Accessed January 25, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823115-001730.

MLA Handbook (7^th Edition):

Huang, Shu-Mei. “Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing.” 2015. Web. 25 Jan 2021.

Vancouver:

Huang S. Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing. [Internet] [Doctoral dissertation]. NSYSU; 2015. [cited 2021 Jan 25]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823115-001730.

Council of Science Editors:

Huang S. Effects of high glucose on keratinocyte functions: focusing on the impaired diabetic wound healing. [Doctoral Dissertation]. NSYSU; 2015. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823115-001730

4. Vargues, Thomas. Antimicrobial peptides : structure, function and resistance.

Degree: PhD, 2009, University of Edinburgh

URL: http://hdl.handle.net/1842/4076

► Higher eukaryotes produce a vast range of antimicrobial peptides (AMPs) that play important roles in their defence against microbial infection. Beta defensins are small (3-5… (more)

▼ Higher eukaryotes produce a vast range of antimicrobial peptides (AMPs) that play important roles in their defence against microbial infection. Beta defensins are small (3-5 kDa), cationic peptides that display broad, potent antimicrobial activity against a range of microbes and also act as chemoattractants of important immunomodulatory cells. To generate highly pure peptides for structural and functional studies, we developed a method to prepare recombinant human beta defensin-2 (HBD2). The HBD2 gene was synthesised by recursive PCR with codons optimised for expression in Escherichia coli. HBD2 was expressed as an insoluble fusion to a His-tagged ketosteroid isomerase. After cleavage from the fusion with cyanogen bromide, 1H NMR spectroscopy and mass spectrometry confirmed that the oxidised HBD2 was folded and possessed the correct b-defensin disulfide bond topology. The recombinant HBD2 was active against E. coli, P. aeruginosa, S. aureus and C. albicans and was also a chemoattractant against HEK293 cells expressing the chemokine receptor CCR6. 15N-labelled HBD2 was also prepared and was highly suitable for future structural studies. Since defensins are thought to interact with bacterial membranes we also tested the recombinant HBD2 in biophysical studies (surface plasmon resonance, SPR, Biacore). We observed different binding to artificial model membranes containing either E. coli Kdo2-lipid A or phospholipids. Bacterial resistance to AMPs has been linked to the covalent modification of the outer membrane lipid A by 4-amino-4-deoxy-L-arabinose (L-Ara4N). This neutralises the charge of the LPS, thereby decreasing the electrostatic attraction of cationic peptides to the bacterial membrane. The pathogen Burkholderia cenocepacia displays extremely high resistance to AMPs and other antibiotics and the Ara4N pathway appears to be essential. To explore this further we expressed recombinant forms of two enzymes (ArnB and ArnG) from the B. cenocepacia Ara4N pathway. Purified ArnB is a pyridoxal 5’-phosphate (PLP)-dependent transaminase and we tested its ability to bind amino acid substrates. We investigated the binding of inhibitors L- and D-cycloserine to ArnB and tested their antibiotic activity against Burkholderia strains. We also studied the B. cenocepacia ArnG – a proposed membrane protein undecaprenyl-L-Ara4N flippase – and showed that the protein behaved as a dimer by non-denaturing gel analysis. The B. cenocepacia ArnG failed to complement E. coli knock-out strains encoding the equivalent flippase proteins ArnE and ArnF, suggesting that ArnG is a Burkholderia-specific protein.

Subjects/Keywords: 572.6; antimicrobial peptides; bacterial resistance; human beta defensin-2; HBD2

…the Mature Recombinant Human β-Defensin 2 (HBD2) 23 2.1 Cloning of HBD2 24 2.2… …Overexpression and Purification of KSI-HBD2 Fusion Protein 25 2.3 CNBr Cleavage of KSI-HBD2 Fusion… …Protein and Purification of HBD2 Using a Fast Protein Chromatography Liquid (FPLC)… …System 26 2.4 Expression and Purification of HBD2 Using the pET-28a/HBD2 Plasmid and an HPLC… …Connectivity for HBD2 32 2.7 Structural Characterisation of Mature -Defensins 34 2.8 Expression…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vargues, T. (2009). Antimicrobial peptides : structure, function and resistance. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/4076

Chicago Manual of Style (16^th Edition):

Vargues, Thomas. “Antimicrobial peptides : structure, function and resistance.” 2009. Doctoral Dissertation, University of Edinburgh. Accessed January 25, 2021. http://hdl.handle.net/1842/4076.

MLA Handbook (7^th Edition):

Vargues, Thomas. “Antimicrobial peptides : structure, function and resistance.” 2009. Web. 25 Jan 2021.

Vancouver:

Vargues T. Antimicrobial peptides : structure, function and resistance. [Internet] [Doctoral dissertation]. University of Edinburgh; 2009. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/1842/4076.

Council of Science Editors:

Vargues T. Antimicrobial peptides : structure, function and resistance. [Doctoral Dissertation]. University of Edinburgh; 2009. Available from: http://hdl.handle.net/1842/4076

5. Capewell, Samantha Jessica. Structural and functional studies of protein targets at the host-pathogen interface.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/9636

► Ferric ABC Transporters. Pathogenic bacteria have evolved specialised iron acquisition systems that allow them to effectively colonise a host. One of these systems is the… (more)

▼ Ferric ABC Transporters. Pathogenic bacteria have evolved specialised iron acquisition systems that allow them to effectively colonise a host. One of these systems is the ferric binding protein (Fbp) complex that is a member of the ATP-Binding Cassette (ABC) superfamily of small molecule transporters. The Fbp complex is made up of three-components (FbpABC) that transports ferric iron from the periplasm to the cytoplasm of many Gram negative bacteria. FbpA binds iron in the periplasm and transports it to the FbpB transporter complex that permeates the cytoplasmic membrane. Here the iron is actively transported by FbpB through the membrane that is powered by ATP hydrolysis catalysed by FbpC, the cytoplasmic ATPase. Burkholderia cenocepacia is an opportunist pathogen that colonises the lungs of cystic fibrosis patients and is particularly resistant to antibiotic treatment. In this study the iron uptake system of B. cenocepacia strain J2315 is investigated. A putative FbpA from B. cenocepacia J2315 was expressed in the periplasm of Escherichia coli cells and the recombinant FbpA B. cenocepacia protein purified. The structural and electrochemical properties of native FbpA B. cenocepacia were investigated using UV Visible spectroscopy, spectro-electrochemistry, mass spectrometry and crystallographic techniques. It appears that FbpA B. cenocepacia is a novel member of the FbpA superfamily that selectively utilises citrate as an exogenous anion in ferric iron co-ordination. This is the first instance that a recombinant ferric binding protein has been documented as preferentially utilising citrate in this manner. The putative ATPase from B. cenocepacia (FbpC B. cenocepacia) was also expressed in E. coli but it was found to be insoluble. A number of expression systems were tested but none were found to be successful in generating sufficient quantities of FbpC B. cenocepacia for structural studies. Human β-defensin 2. Despite daily contact with a range of microorganisms, mammals do not regularly succumb to pathogenic invasion. One reason is the presence of an important defence mechanism uses a reservoir of antimicrobial peptides (AMPs) that are expressed in eukaryotes as a means of innate immunity. The AMP superfamily is composed of over 900 members, displays broad structural and sequence diversity and is active against a wide range of bacteria, fungi and viruses. β-defensins are small (3-5 kDa), cationic peptides that display antimicrobial activity against a range of microbes and have also been shown to act as chemo-attractants (chemokines) within the adaptive immune system. In this study we obtained milligram amounts of pure human β-defensin 2 (HBD2) for functional studies by the development of a method for the rapid expression and purification of the recombinant peptide. A clone encoding a thioredoxin-HBD2 fusion protein was designed for the expression of soluble peptide in E. coli cells that was purified by simple affinity chromatography. The HBD2 peptide was cleaved from the fusion by an efficient protease step and further…

Subjects/Keywords: 572; ferric binding protein; iron uptake system; B. cenocepacia; recombinant HBD2 defensin

…66 3.6.1 Construction of pET32b Thioredoxin-‐HBD2 fusion protein… …66 3.6.2 pET32b Thioredoxin TEV HBD2 with an N-‐terminal His6… …tag. ................................. 66 3.6.3 Expression of Trx-‐HBD2… …68 3.6.8 TEV Cleavage of Trx-‐HBD2… …68 3.6.9 Purification of HBD2 by Nickel IMAC…

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APA (6^th Edition):

Capewell, S. J. (2014). Structural and functional studies of protein targets at the host-pathogen interface. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/9636

Chicago Manual of Style (16^th Edition):

Capewell, Samantha Jessica. “Structural and functional studies of protein targets at the host-pathogen interface.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed January 25, 2021. http://hdl.handle.net/1842/9636.

MLA Handbook (7^th Edition):

Capewell, Samantha Jessica. “Structural and functional studies of protein targets at the host-pathogen interface.” 2014. Web. 25 Jan 2021.

Vancouver:

Capewell SJ. Structural and functional studies of protein targets at the host-pathogen interface. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Jan 25]. Available from: http://hdl.handle.net/1842/9636.

Council of Science Editors:

Capewell SJ. Structural and functional studies of protein targets at the host-pathogen interface. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/9636

6. 五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.

Degree: 博士（歯学）, 2015, Nihon University / 日本大学

URL: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Subjects/Keywords: 電解酸性機能水; electrolyticaly-generated acid functional water; 2 本鎖 RNA; double-stranded RNA; ルシフェラーゼアッセイ; luciferase assay; hBD2; human β-defensin 2; IL-8; interleukin 8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

五條堀,孝廣. (2015). Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. (Thesis). Nihon University / 日本大学. Retrieved from http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

五條堀,孝廣. “Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.” 2015. Thesis, Nihon University / 日本大学. Accessed January 25, 2021. http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

五條堀,孝廣. “Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路.” 2015. Web. 25 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. [Internet] [Thesis]. Nihon University / 日本大学; 2015. [cited 2021 Jan 25]. Available from: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

五條堀,孝廣. Signaling pathways of electroliticaly-generated acid functional water in epithelial cells : 上皮細胞における電解酸性機能水のシグナル伝達経路. [Thesis]. Nihon University / 日本大学; 2015. Available from: http://repository.nihon-u.ac.jp/xmlui/handle/11263/554 ; http://dx.doi.org/10.15006/32665A4948

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

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