subject:(granulosa cells)

University of Guelph

1. Toms, Derek Douglas. Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation.

Degree: PhD, Department of Animal and Poultry Science, 2014, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8305

► Within the developing antral follicle, several factors are required for successful maturation of both the oocyte and the granulosa cells. It has been repeatedly shown… (more)

▼ Within the developing antral follicle, several factors are required for successful maturation of both the oocyte and the granulosa cells. It has been repeatedly shown that oocytes isolated from smaller follicles have considerably less developmental potential than those isolated from more mature, large follicles, despite both types typically being capable of resuming meiosis. Research in our laboratory has previously identified glial cell line-derived neurotrophic factor (GDNF) as an important intraovarian factor that can improve oocyte development and embryo potential of both small and large follicle-derived oocytes when included in in vitro maturation (IVM) media. This thesis examined the transcriptomic changes that occur within the oocyte when cultured in the absence or presence of GDNF and how these relate to the size of the follicle from which the oocyte originates. This research was undertaken using a microarray specific to porcine oocytes and early embryos. We found that GDNF made the transcriptome of small follicle-derived oocytes more similar to that of large follicle-derived oocytes. Additionally, the expression of many genes in response to GDNF was found to have significant follicle size-dependent effects. We also examined the role of microRNAs (miRNAs) in follicular development by examining potential regulatory mechanisms on GDNF and progesterone receptor (PGR). While no significant miRNA-mediated inhibition of GDNF expression was found, we did establish a role for miR-378 in regulating PGR. The transcription factor activity of PGR regulates a number of gene products that are necessary for remodeling the follicular extracellular matrix and for releasing cytokines. Over expression of miR-378 decreased mRNA and protein levels of PGR, and congruently mRNA levels of several genes known to be regulated by PGR and important for follicular development. Interestingly, not only did miR-378 regulate expression of PGR, this miRNA was itself regulated by the pituitary hormone follicle-stimulating hormone (FSH). Taken together, we found that FSH mediates follicular differentiation by regulating expression of PGR, at least in part via the down regulation of miR-378. In examining the role of GDNF and miR-378 in cells of the antral follicle, this thesis has provided additional information for the mechanisms involved in follicle maturation. Both GDNF and miR-378 regulate important developmental processes that are necessary for successful follicle development to produce competent oocytes. As many species, including humans, have a limited pool of large, fully developed follicles understanding the mechanisms that increase oocyte developmental competence will be important for the advancement of assisted reproductive technologies. Advisors/Committee Members: Li, Julang (advisor).

Subjects/Keywords: GDNF; granulosa cells; microRNA-378; ovary; pig

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Toms, D. D. (2014). Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8305

Chicago Manual of Style (16^th Edition):

Toms, Derek Douglas. “Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation.” 2014. Doctoral Dissertation, University of Guelph. Accessed March 08, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8305.

MLA Handbook (7^th Edition):

Toms, Derek Douglas. “Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation.” 2014. Web. 08 Mar 2021.

Vancouver:

Toms DD. Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation. [Internet] [Doctoral dissertation]. University of Guelph; 2014. [cited 2021 Mar 08]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8305.

Council of Science Editors:

Toms DD. Involvement of GDNF and microRNA-378 in the regulation of porcine follicle maturation. [Doctoral Dissertation]. University of Guelph; 2014. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8305

University of Illinois – Chicago

2. Baumgarten, Sarah Catherine. Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation.

Degree: 2017, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/21843

► Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation Infertility affects more than 7% of couples in the United States. Approximately… (more)

Subjects/Keywords: cumulus granulosa cells; FSH; IGF2; IGF1R

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baumgarten, S. C. (2017). Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baumgarten, Sarah Catherine. “Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation.” 2017. Thesis, University of Illinois – Chicago. Accessed March 08, 2021. http://hdl.handle.net/10027/21843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baumgarten, Sarah Catherine. “Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation.” 2017. Web. 08 Mar 2021.

Vancouver:

Baumgarten SC. Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10027/21843.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baumgarten SC. Role of Insulin-like Growth Factors in Granulosa Cell Differentiation and Ovarian Follicle Maturation. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21843

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Santa Maria

3. Rogério Ferreira. AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS.

Degree: 2010, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3509

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The objectives of the present study was to determine the concentration of angiotensin II (AngII) in follicular fluid, to characterize the expression of renin-angiotensin system… (more)

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The objectives of the present study was to determine the concentration of angiotensin II (AngII) in follicular fluid, to characterize the expression of renin-angiotensin system (RAS) genes in follicular cells and to verify the role of AngII in the follicular wave, using an in vivo model with intrafollicular injection and in vitro model with granulosa cells culture. AngII concentration in follicular fluid increased on dominant follicle during and after deviation. Saralasin inhibited follicular growth in all treated cows (4/4), suggesting that AngII is essential for follicular growth in 7-8mm follicles. However, intrafollicular injection of AngII affected neither follicular growth nor the pattern of follicular dynamics, which were similar to control cows. These results imply that bovine ovarian follicles contain sufficient AngII for follicle development. In another experiment, saralasin inhibitory effect was overcome by systemic FSH supplementation, suggesting that AngII is essential to follicular growth during FSH-low dependence stages. The injection of AngII or angiotensin receptor type 2 (AGTR2) agonist in second largest follicle prevented the expected atresia of subordinate follicle and the treated follicle grew at the same rate as the dominant during 24h. To understand why a single intrafollicular injection of AngII antagonist induces follicular atresia, dominant follicle was injected with saralasin or saline and the cows were ovariectomized 24h later. The inhibition of AngII action decreased estradiol concentration in follicular fluid and abundance of mRNA encoding aromatase (CYP19), 3!-hydroxysteroid dehydrogenase (3!HSD), LH receptor, SerpinE2 and cyclinD2 in granulosa cells. On granulosa cell culture, AngII increased CYP19 expression just in the presence of FSH. Taken together, these results show that AngII is essential for follicular growth, and plays important role in regulating genes involved in granulosa cell proliferation (cyclinD2) and differentiation (LHr, aromatase, 3!HSD), which are necessary for development of the dominant follicle. In conclusion, these data suggest that AngII signaling is involved in follicle growth and dominance in cattle.

Os objetivos do presente trabalho foram determinar a concentração de angiotensina II (AngII) no fluido folicular, caracterizar a expressão dos genes do sistema renina-angiotensina (RAS) nas células foliculares e verificar o papel da AngII na onda folicular, utilizando um modelo in vivo de injeção intrafolicular e in vitro de cultivo de células da granulosa. A concentração de AngII no fluido folicular aumentou no folículo de maior diâmetro, durante e após a divergência folicular. A administração intrafolicular de saralasina, um bloqueador competitivo dos receptores de AngII, inibiu o crescimento folicular em todas as vacas injetadas (4/4), demonstrando que a AngII é essencial para o crescimento de folículos de 7-8mm de diâmetro. Contudo, a injeção de AngII não afetou o crescimento folicular, sugerindo que os folículos contém AngII suficiente para o seu…

Advisors/Committee Members: Paulo Bayard Dias Goncalves, Vilceu Bordignon, Marlon Nadal Maciel, Luiz Ernani Henkes, Adelina Martha dos Reis.

Subjects/Keywords: esteroidogênese; granulosa; fatores foliculares; divergência folicular; MEDICINA VETERINARIA; follicular deviation; intrafollicular factors; granulosa cells; steroidogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ferreira, R. (2010). AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ferreira, Rogério. “AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS.” 2010. Thesis, Universidade Federal de Santa Maria. Accessed March 08, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ferreira, Rogério. “AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS.” 2010. Web. 08 Mar 2021.

Vancouver:

Ferreira R. AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2010. [cited 2021 Mar 08]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3509.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ferreira R. AÇÃO DA ANGIOTENSINA II NA REGULAÇÃO DA DOMINÂNCIA FOLICULAR EM BOVINOS. [Thesis]. Universidade Federal de Santa Maria; 2010. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=3509

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Reverchon, Maxime. Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available.

Degree: Docteur es, Sciences de la vie, 2014, Université François-Rabelais de Tours

URL: http://www.theses.fr/2014TOUR4036

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Les aclipocytokines (aclipo), produites par le tissu adipeux jouent un rôle clé dans la régulation des fonctions métaboliques mais qu'en est-il pour les fonctions de… (more)

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Les aclipocytokines (aclipo), produites par le tissu adipeux jouent un rôle clé dans la régulation des fonctions métaboliques mais qu'en est-il pour les fonctions de reproduction? Nous montrons que la chemerine et ses récepteurs, la visfatine et la résistine sont présents dans les cellules ovariennes humaines et bovines. ln vitro nous observons que la chemerine et la résistine diminuent la stéroïdogenèse des cellules de la granulosa (CG) humaine induite par IGF-1 alors que la visfatine l'augmente. Des résultats similaires sont observés chez la vache pour la chemerine et la visfatine. Dans les deux espèces, les aclipo influencent la prolifération des CG, et les voies de signalisation AKT, MAPK-ERKl/2 et P38 ou l'AMPK. Chez le bovin, la chemerine bloque la maturation ovocytaire in vitro. Nous observons aussi dans cette espèce que la concentration plasmatique de résistine et son expression dans les aclipocytes est augmentée après vêlage lorsque la lipomobilisation est élevée. Ces travaux confirment le rôle de la résistine dans la régulation métabolique chez la vache et montrent l'importance des adipo dans les cellules ovariennes humaine et bovine. Il reste à élargir leur rôle au niveau central dans les fonctions de reproduction.

The aclipokines (aclipo ), produced by the adipose tissue play a key role in the regulation of metabolic functions, but what about for reproductive functions? We show that chemerin and its receptors, visfatin and resistin are present in human and bovine ovary cells. ln vitro we observe that chemerin and resistin decrease steroidogenesis in granulosa cells (GC) in response to IGF-1 while visfatin increases it. Similar results are observed for chemerin and visfatin in cows. In both species, chemerin, visfatin and resistin affect the proliferation of CG and signaling pathways inclucling AKT, MAPKERKl I 2 and P38 or AMPK. In cattle, chemerin blocks in vitro oocyte maturation. In this species, we also observe that the plasma resistin and its expression in aclipocytes are increased after calving when the fatty acid mobilization is high. This work confirms the role of resistin in the metabolic regulations in cow and shows the importance of aclipo in the human and bovine ovary cells. It remains to investigate their role at the central level in the reproductive functions.

Advisors/Committee Members: Dupont, Joëlle (thesis director).

Subjects/Keywords: Adipocytokines; Cellules de la granulosa; Stéroïdogenèse; Maturation ovocytaire; Aclipokines; Granulosa cells; Steroidogenesis; Oocyte maturation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reverchon, M. (2014). Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2014TOUR4036

Chicago Manual of Style (16^th Edition):

Reverchon, Maxime. “Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available.” 2014. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 08, 2021. http://www.theses.fr/2014TOUR4036.

MLA Handbook (7^th Edition):

Reverchon, Maxime. “Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available.” 2014. Web. 08 Mar 2021.

Vancouver:

Reverchon M. Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2014. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2014TOUR4036.

Council of Science Editors:

Reverchon M. Identification et rôle in vitro de la chemerine, résistine et visfatine dans l'ovaire humain et bovin : No title available. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2014. Available from: http://www.theses.fr/2014TOUR4036

5. Maqdasy, Salwan. Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads.

Degree: Docteur es, Génétique et Physiologie Moléculaire, 2016, Université Blaise-Pascale, Clermont-Ferrand II

URL: http://www.theses.fr/2016CLF22713

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La stérilité affecte à l’heure actuelle près de 15-20 % des couples et sa prévalence est en progression depuis quatre à cinq décennies. Cette progression évolue… (more)

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La stérilité affecte à l’heure actuelle près de 15-20 % des couples et sa prévalence est en progression depuis quatre à cinq décennies. Cette progression évolue parallèlement à la prévalence de l’épidémie d’obésité et de syndrome métabolique dans le monde. De multiples arguments physiologiques et épidémiologiques chez l’Homme soutiennent l’hypothèse de l’influence de l’homéostasie des lipides sur la fonction gonadique. En particulier, le cholestérol est un facteur clef dans la régulation de la stéroïdogenèse et de la gamétogenèse. Bien que les atteintes gonadiques semblent multifactorielles, les mécanismes moléculaires restent méconnus dans la majorité des cas. Les Liver X receptors (LXRα et β) sont des récepteurs nucléaires activés par les oxystérols. Ce sont classiquement des régulateurs du métabolisme lipidique. Plusieurs études ont démontré l’importance de ces récepteurs dans la physiologie des gonades. Ce travail identifie les rôles multiples des LXRs dans le maintien de la fertilité masculine et féminine, et décrit l’effet de l’homéostasie du cholestérol sur la maturation des cellules germinales dans le testicule et l’ovaire. Ce travail se concentre sur l’analyse comparative d’une lignée de souris ré-exprimant LXRβ (Lxrαϐ-/-:AMHLxrϐ) sous contrôle de promoteur d’AMH humain (expression spécifique dans les cellules de Sertoli dans le testicule et les cellules de granulosa dans l’ovaire) sur un fond génétique de souris Lxrαϐ-/-. L’absence d’un isoforme d LXR aboutit à des défauts spécifiques dans un type cellulaire du testicule. Néanmoins, le dysfonctionnement d’un type cellulaire est compensé. En effet, de multiples défauts sont nécessaires pour aboutir à la stérilité. LXR dans les cellules de granulosa est critique pour la maturation et la survie des ovocytes, l’ovulation, et par conséquence pour la fertilité. Ainsi, LXRβ est une cible potentielle pour réguler la fertilité féminine et la prévention de syndrome d’hyperstimulation ovarienne. Nos résultats ouvrent des perspectives pour des nouvelles cibles diagnostiques et pronostiques dans la fertilité.

Sterility affects 15 % of French population and its prevalence is propagating since four or five decades. Many human physiological and epidemiological arguments support the impact of lipid homeostasis on the gonads; indeed, cholesterol is a key regulator of steroidogenesis and gametogenesis. Nevertheless, the molecular mechanisms remain hidden. Liver X receptors alpha and beta (LXRα and β) belong to the superfamily of nuclear receptors and are activated upon binding to oxysterols. LXRs are mainly implicated in cholesterol homeostasis. Increasing bulk of literature identified these non-steroid nuclear receptors as major regulators of the gonad physiology. This work uncovers previously unidentified putative roles of LXRs and ability of cholecterol excess to alter male and female germ cell maturation. Herein, we analyse a new mouse strain (Lxrαϐ-/-:AMHLxrϐ) re-expressing LXRβ under control of AMH promoter (specific to Sertoli in testis and granulosa cells in ovary)…

Advisors/Committee Members: Baron, Silvère (thesis director).

Subjects/Keywords: LXR; Cholestérol; Fertilité; Cellules de Sertoli; Cellules de Granulosa; LXR; Cholesterol; Fertility; Sertoli cells; Granulosa cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maqdasy, S. (2016). Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads. (Doctoral Dissertation). Université Blaise-Pascale, Clermont-Ferrand II. Retrieved from http://www.theses.fr/2016CLF22713

Chicago Manual of Style (16^th Edition):

Maqdasy, Salwan. “Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads.” 2016. Doctoral Dissertation, Université Blaise-Pascale, Clermont-Ferrand II. Accessed March 08, 2021. http://www.theses.fr/2016CLF22713.

MLA Handbook (7^th Edition):

Maqdasy, Salwan. “Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads.” 2016. Web. 08 Mar 2021.

Vancouver:

Maqdasy S. Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads. [Internet] [Doctoral dissertation]. Université Blaise-Pascale, Clermont-Ferrand II; 2016. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2016CLF22713.

Council of Science Editors:

Maqdasy S. Implication de "Liver X Receptors" dans la physiopathologie des gonades : Implication of « Liver X Receptors » in the physiopathology of gonads. [Doctoral Dissertation]. Université Blaise-Pascale, Clermont-Ferrand II; 2016. Available from: http://www.theses.fr/2016CLF22713

6. 岩宗, 政幸. MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する.

Degree: 博士（医学）, 2014, Gunma University / 群馬大学

URL: http://hdl.handle.net/10087/8616

学位記番号：医博甲1483

Subjects/Keywords: microRNA; GRP78; granulosa cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

岩宗, �. (2014). MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する. (Thesis). Gunma University / 群馬大学. Retrieved from http://hdl.handle.net/10087/8616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

岩宗, 政幸. “MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する.” 2014. Thesis, Gunma University / 群馬大学. Accessed March 08, 2021. http://hdl.handle.net/10087/8616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

岩宗, 政幸. “MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する.” 2014. Web. 08 Mar 2021.

Vancouver:

岩宗 �. MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する. [Internet] [Thesis]. Gunma University / 群馬大学; 2014. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10087/8616.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

岩宗 �. MicroRNA-376a Regulates 78-Kilodalton Glucose-Regulated Protein Expression in Rat Granulosa Cells : ラット顆粒膜細胞においてmiR-376aはGRP78の発現を調節する. [Thesis]. Gunma University / 群馬大学; 2014. Available from: http://hdl.handle.net/10087/8616

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

7. Behrouzi, Amir. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.

Degree: MS, Department of Agricultural, Food, and Nutritional Science, 2014, University of Alberta

URL: https://era.library.ualberta.ca/files/6w924c30w

► In previous research, the use of porcine luteinizing hormone (pLH) in lieu of gonadotropin-releasing hormone (GnRH) for synchronizing ovulation in a fixed-timed artificial insemination protocol… (more)

Subjects/Keywords: Follicle; Granulosa cells; Dairy cows; Pregnancy rate; Luteinizing hormone; Oocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Behrouzi, A. (2014). Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/6w924c30w

Chicago Manual of Style (16^th Edition):

Behrouzi, Amir. “Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.” 2014. Masters Thesis, University of Alberta. Accessed March 08, 2021. https://era.library.ualberta.ca/files/6w924c30w.

MLA Handbook (7^th Edition):

Behrouzi, Amir. “Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows.” 2014. Web. 08 Mar 2021.

Vancouver:

Behrouzi A. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2021 Mar 08]. Available from: https://era.library.ualberta.ca/files/6w924c30w.

Council of Science Editors:

Behrouzi A. Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/6w924c30w

Univerzitet u Beogradu

8. Samardžija Nenadov, Dragana D., 1987-. Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova.

Degree: Biološki fakultet, 2017, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:16821/bdef:Content/get

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Biologija - Reproduktivna fiziologija i toksikologija / Biology - Reproductive physiology and toxicology

Atrazin (ATR), jedan od najčešće korišćenih herbicida, i bisfenol A (BPA), hemikalija… (more)

Subjects/Keywords: granulosa cells; atrazine; bisphenol A; steroidogenesis; ovulation; cholesterol

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Samardžija Nenadov, Dragana D., 1. (2017). Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:16821/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Samardžija Nenadov, Dragana D., 1987-. “Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova.” 2017. Thesis, Univerzitet u Beogradu. Accessed March 08, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:16821/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Samardžija Nenadov, Dragana D., 1987-. “Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova.” 2017. Web. 08 Mar 2021.

Vancouver:

Samardžija Nenadov, Dragana D. 1. Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2017. [cited 2021 Mar 08]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:16821/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Samardžija Nenadov, Dragana D. 1. Uticaj atrazina i bisfenola A na funkciju granuloza ćelija ovarijuma pacova. [Thesis]. Univerzitet u Beogradu; 2017. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:16821/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Prapa, Ermioni - Dimitra. Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού.

Degree: 2016, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας

URL: http://hdl.handle.net/10442/hedi/37280

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Effect of Anti-Müllerian hormone (AMH) and bone morphogenetic protein 15 (BMP-15) on steroidogenesis in primary-cultured human luteinized granulosa cells through Smad5 signalling.Purpose: To determine if… (more)

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Effect of Anti-Müllerian hormone (AMH) and bone morphogenetic protein 15 (BMP-15) on steroidogenesis in primary-cultured human luteinized granulosa cells through Smad5 signalling.Purpose: To determine if there is any effect of AMH and BMP-15 on estradiol and progesterone production from primary-cultured human luteinized granulosa cells, to delineate what is the effect of FSH on their actions and which are the possible mechanisms involved. Methods: Luteinized granulosa cells (GCs), obtained from follicular fluid of 30 women undergoing in vitro fertilization, were cultured, after a short 24-hour preincubation period, in serum-free medium for 24 or/and 48 hours in the presence/absence of various concentrations of AMH, BMP-15 and FSH alone or in combinations. Estradiol and progesterone production, SMAD5 phosphorylation and StAR expression were studied in parallel. Steroids were measured in culture-supernatant using enzyme-immunoassays, while Smad5-signaling pathway activation and StAR protein expression were assessed immunocytochemically.Result(s): We found that the treatment of AMH in GCs for 24/48 hours attenuated FSH-induced estradiol production (p<0.001), had no effect on basal estradiol levels, decreased basal progesterone production (p<0.001) and FSH-induced StAR expression (p<0.001). On the other hand, BMP-15 decreased basal estradiol levels (p<0.001) and attenuated FSH-induced estradiol production (p<0.001). Furthermore, BMP-15 reduced progesterone basal secretion (p<0.001), an effect that was partially reversed by FSH (p<0.01), probably via increasing StAR expression (p<0.001). FSH-induced StAR expression was also attenuated by BMP-15 (p<0.001). FSH, AMH and BMP-15 activated Smad-signaling pathway, as confirmed by the increase of phospo-Smad5 protein levels (p<0.001 compared to control).Conclusion(s): AMH and BMP-15 by interacting with FSH affect the production of estradiol and progesterone from cultured luteinized-granulosa cells possibly via Smad5-protein phosphorylation.

Η παρούσα εργασία πραγματοποιήθηκε στην προσπάθεια διερεύνησης των δράσεων της αντιμυλλέριας ορμόνης (ΑΜΗ) και της οστικής μορφογενετικής πρωτεΐνης -15 (ΒΜΡ-15) στην παραγωγή οιστραδιόλης (Ε2) και προγεστερόνης (Ρ4) από πρωτογενή ανθρώπινα ωχρινοποιημένα κύτταρα απουσία ή παρουσία της ορμόνης FSH. Τα εμπλεκόμενα ενδοκυττάρια μονοπάτια και οι μηχανισμοί δράσεις αποτέλεσαν επίσης αντικείμενο μελέτης. Για το σκοπό αυτό, χρησιμοποιήθηκαν, κατόπιν γραπτής συναίνεσης, ανθρώπινα ωχρινοποιημένα κύτταρα, τα οποία απομονώθηκαν από το ωοθυλακικό υγρό 30 γυναικών, που υποβλήθηκαν σε πρόγραμμα υποβοηθούμενης αναπαραγωγής. Τα κοκκώδη κύτταρα, καλλιεργήθηκαν, μετά από μία περίοδο προεπώασης 24 ωρών, σε καλλιεργητικό υλικό χωρίς ορό παρουσία η απουσία διαφορετικών συγκεντρώσεων των ορμονών ΑΜΗ, ΒΜΡ-15 και FSH, μεμονωμένα η σε συνδυασμό. Η παραγωγή Ε2 και Ρ4, καθώς επίσης η φωσφορυλίωση της πρωτεΐνης Smad5 και η έκφραση της πρωτεΐνης StAR, μελετήθηκαν παράλληλα. Οι στεροειδείς ορμόνες μετρήθηκαν ανοσοενζυμικά στο υπερκείμενο των καλλιεργειών, ενώ η…

Subjects/Keywords: Στεροειδογένεση; Κοκκώδη κύτταρα; Άνθρωπος; AMH; BMP-15; Steroeidogenesis; Granulosa cells; Human

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prapa, E. -. D. (2016). Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού. (Thesis). University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Retrieved from http://hdl.handle.net/10442/hedi/37280

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prapa, Ermioni - Dimitra. “Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού.” 2016. Thesis, University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας. Accessed March 08, 2021. http://hdl.handle.net/10442/hedi/37280.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prapa, Ermioni - Dimitra. “Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού.” 2016. Web. 08 Mar 2021.

Vancouver:

Prapa E-D. Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού. [Internet] [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2016. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10442/hedi/37280.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prapa E-D. Επίδραση της αντιμυλλεριανικής ορμόνης (ΑΜΗ) και της μορφογενετικής πρωτεΐνης 15 (BMP-15) στη στεροειδογένεση από πρωτογενείς καλλιέργειες ανθρώπινων κοκκωδών κυττάρων μέσω του Smad5 σηματοδοτικού μονοπατιού. [Thesis]. University of Thessaly (UTH); Πανεπιστήμιο Θεσσαλίας; 2016. Available from: http://hdl.handle.net/10442/hedi/37280

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

10. Bouilly, Justine. Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology.

Degree: Docteur es, Ascpects moléculaires et cellulaires de la biologie, 2014, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2014PA11T069

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Les données bibliographiques décrivent un nombre croissant de modèles murins caractérisés sur le plan de la fertilité permettant une meilleure compréhension du phénomène de la… (more)

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Les données bibliographiques décrivent un nombre croissant de modèles murins caractérisés sur le plan de la fertilité permettant une meilleure compréhension du phénomène de la croissance folliculaire. Certains de ces modèles animaux, invalidés pour des facteurs de transcription reproduisent un phénotype d’infertilité, telle que l’insuffisance ovarienne primaire (IOP). La compréhension des mécanismes moléculaires des facteurs de transcription essentiels à la fonction ovarienne n’est pas clairement établie. Les protéines contenant des domaines liant l’ADN, tels que les homéodomaines ou les domaines forkhead jouent un rôle-Clé dans le développement ovarien. NOBOX (Newborn Ovary Homeobox) est un facteur de transcription essentiel à la mise en place du stock folliculaire, et dont les mutations sont responsables d’IOP. La fonction exacte de NOBOX n’est pas connue, s’il est présent dans l’ovocyte, nous montrons pour la première fois une forte expression de cette protéine dans les cellules de la granulosa des follicules primordiaux jusqu’au stade secondaire. De plus, par différentes techniques moléculaires, nous mettons en évidence une interaction entre NOBOX et un autre acteur important de la folliculogenèse FOXL2 (Forkhead box l2), contribuant à la régulation de leurs gènes cibles respectifs. Cette étude permet de mettre en lumière le rôle de NOBOX dans les cellules de granulosa. L’IOP est une pathologie touchant 1 % des femmes âgées de moins de 40 ans. Sur le plan ovarien, il y a une déplétion du stock des follicules ou un blocage de la maturation folliculaire. De ce fait, la stérilité est le plus souvent définitive. Une origine génétique de cette maladie est parfois retrouvée avec des mutations des autosomes et/ou du chromosome X, mais dans plus de 80% des cas l’IOP est idiopathique. L’enjeu est donc d’identifier de nouveaux gènes candidats pour cette pathologie. Dans cette étude nous validons la prévalence des mutations du gène NOBOX faisant de ce facteur un des gènes clés de l’IOP. Puis, à l’aide d’une nouvelle technologie : le séquençage multiplex par puces PGMTM ION TORRENT, nous mettons en évidence dans 26% de la cohorte étudiée (100 femmes atteintes d’IOP sporadique primaire ou secondaire) un défaut génétique de 10 gènes, dont 4 nouveaux candidats à l’IOP. De façon intéressante, la présence d’au moins deux gènes mutés chez 9 patientes induit un phénotype plus précoce. Cette étude contribue à une meilleure compréhension de l’origine génétique de l’IOP et met pour la première fois en évidence le phénomène d’oligogénisme chez des patientes en IOP.

Single germline mutations found in women with primary ovarian insufficiency (POI), besides mouse models have provided substantial understanding into the factors involved in differentiation and ovarian development. POI is characterized by amenorrhea with elevated gonadotropin levels, and affects 1% of women before the age of 40 years.Several transcription factors involved in ovary development and folliculogenesis are mutated in reproductive disorders. We have shown a high…

Advisors/Committee Members: Binart, Nadine (thesis director).

Subjects/Keywords: Ovaire; NOBOX; FOXL2; Cellules de la granulosa; Insuffisance ovarienne primaire; Ovary; FOXL2; NOBOX; Granulosa cells; Primary ovarian insufficiency

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bouilly, J. (2014). Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2014PA11T069

Chicago Manual of Style (16^th Edition):

Bouilly, Justine. “Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology.” 2014. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 08, 2021. http://www.theses.fr/2014PA11T069.

MLA Handbook (7^th Edition):

Bouilly, Justine. “Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology.” 2014. Web. 08 Mar 2021.

Vancouver:

Bouilly J. Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2014. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2014PA11T069.

Council of Science Editors:

Bouilly J. Etude de nouveaux acteurs de la physiopathologie ovarienne : Study of Few Factors in Ovarian Pathophysiology. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2014. Available from: http://www.theses.fr/2014PA11T069

University of Adelaide

11. Pacella-Ince, Leanne. Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age.

Degree: 2014, University of Adelaide

URL: http://hdl.handle.net/2440/101482

► Women with reduced ovarian reserve or advanced maternal age are known to have poorer IVF outcomes compared to younger women with normal ovarian reserve. While… (more)

▼ Women with reduced ovarian reserve or advanced maternal age are known to have poorer IVF outcomes compared to younger women with normal ovarian reserve. While previous studies in humans have correlated metabolite concentrations in follicular fluid to IVF outcome, the impact of maternal age and reduced ovarian reserve have yet to be determined. Oocytes are dependent on mitochondrial metabolism for viability and disruptions to mitochondrial activity can reduce oocyte viability. It has been suggested that oocytes from women with either reduced ovarian reserve or advanced maternal age have a reduction in metabolic function, however, the exact mechanisms behind this reduction remain largely unknown. Interestingly, a family of proteins, the Sirtuins, are able to sense cellular metabolic state and post-translationally alter protein function, thus implicating these proteins in metabolic function. Sirtuin 3 (SIRT3) and Sirtuin 5 (SIRT5) are two proteins that are specifically located to mitochondria, thus may be important in understanding metabolic control in the pre-ovulatory follicle. Thus the aim of this thesis was to determine if a difference exists in the follicular environment in women with reduced ovarian reserve or advanced maternal age and if so does SIRT3 or SIRT5 play role in ovarian follicular cells. Women (n=111) undergoing routine IVF treatment were recruited to participate in this study. They were allocated to one of three cohorts based on maternal age (young maternal age [≤35]; advanced maternal age ≥40) and ovarian reserve for age, as measured by serum anti-mullerian hormone (AMH) levels. Surplus follicular fluid, granulosa and cumulus cells were collected, de-identified and randomly allocated to experimental protocols. Follicular fluid concentrations of carbohydrates (glucose, lactate and pyruvate), hormones FSH, LH, progesterone, estrogen and AMH), and selected ions were determined. Metabolic analysis of granulosa and cumulus cells was performed. Granulosa and cumulus gene expression of phosphofructokinase platelet (PFKP) and lactate dehydrogenase A (LDHA) was determined. SIRT3 and SIRT5 gene expression and protein activity was confirmed in granulosa and cumulus cells via qPCR, immunohistochemistry, western blotting and deacetylation/desuccinylation activity. Granulosa and cumulus cell carbamoyl phosphate synthase I (CPS1) protein, a SIRT5 target, was confirmed using immunohistochemistry. Follicular fluid ammonium concentration and granulosa and cumulus cell glutamate dehydrogenase (GDH) activity, a SIRT3 target, were assessed using microfluorometry. Granulosa and cumulus cell acetylated mitochondrial proteins were separated by immunoprecipitation and acetylation of GDH was assessed via western blotting. Data from young women with normal ovarian reserve were compared with those from young women with reduced ovarian reserve and those of advanced maternal age. Young women with normal ovarian reserve had significantly lower starting FSH doses and fewer previous cycles compared to the remaining two groups.… Advisors/Committee Members: Lane, Michelle Therese (advisor), Zander-Fox, Deirdre (advisor), School of Paediatrics and Reproductive Health (school).

Subjects/Keywords: granulosa cells; cumulus cells; Sirtuin 3; Sirtuin 5; reduced ovarian reserve; advanced maternal age

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pacella-Ince, L. (2014). Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/101482

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pacella-Ince, Leanne. “Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age.” 2014. Thesis, University of Adelaide. Accessed March 08, 2021. http://hdl.handle.net/2440/101482.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pacella-Ince, Leanne. “Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age.” 2014. Web. 08 Mar 2021.

Vancouver:

Pacella-Ince L. Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age. [Internet] [Thesis]. University of Adelaide; 2014. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2440/101482.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pacella-Ince L. Mitochondrial Sirtuin 3 and Sirtuin 5 in granulosa and cumulus cells and their contribution to the altered follicular environment in women with either reduced ovarian reserve or advanced maternal age. [Thesis]. University of Adelaide; 2014. Available from: http://hdl.handle.net/2440/101482

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. WANG XINGANG. Analysis of gonad differentiation in zebrafish by histology and transgenics.

Degree: 2007, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/13410

Subjects/Keywords: zebrafish; gonad; sex; granulosa cells; Sertoli cells; Leydig cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

XINGANG, W. (2007). Analysis of gonad differentiation in zebrafish by histology and transgenics. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/13410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

XINGANG, WANG. “Analysis of gonad differentiation in zebrafish by histology and transgenics.” 2007. Thesis, National University of Singapore. Accessed March 08, 2021. http://scholarbank.nus.edu.sg/handle/10635/13410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

XINGANG, WANG. “Analysis of gonad differentiation in zebrafish by histology and transgenics.” 2007. Web. 08 Mar 2021.

Vancouver:

XINGANG W. Analysis of gonad differentiation in zebrafish by histology and transgenics. [Internet] [Thesis]. National University of Singapore; 2007. [cited 2021 Mar 08]. Available from: http://scholarbank.nus.edu.sg/handle/10635/13410.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

XINGANG W. Analysis of gonad differentiation in zebrafish by histology and transgenics. [Thesis]. National University of Singapore; 2007. Available from: http://scholarbank.nus.edu.sg/handle/10635/13410

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. F. Pizzo. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.

Degree: 2015, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/258584

► Information on the potential effects of fusariotoxins on reproductive system in ruminants are few. Therefore, the purpose of this study is to evaluate if DON… (more)

Subjects/Keywords: mycotoxins; granulosa cells; bovine; zearalenone; deoxynivalenol; steroidogenesis; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pizzo, F. (2015). IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/258584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Thesis, Università degli Studi di Milano. Accessed March 08, 2021. http://hdl.handle.net/2434/258584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pizzo, F.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS.” 2015. Web. 08 Mar 2021.

Vancouver:

Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Internet] [Thesis]. Università degli Studi di Milano; 2015. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2434/258584.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pizzo F. IN VITRO TOXICOLOGICAL EFFECTS OF FUSARIUM MYCOTOXINS ON BOVINE GRANULOSA CELLS. [Thesis]. Università degli Studi di Milano; 2015. Available from: http://hdl.handle.net/2434/258584

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

14. Li, Dongxing. Oxygen consumption of bovine granulosa cells in vitro.

Degree: M. Eng., Biotechnology, 2012, Massey University

URL: http://hdl.handle.net/10179/3986

► The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte… (more)

▼ The oxygen consumption rate of granulosa cells is considered to be a key determinant of oocyte oxygenation in follicles. The oxygen status of the oocyte potentially dictates its developmental competence. However, quantitative information on the oxygen consumption rate of granulosa cells in literature is scarce. This limitation has hindered further investigation into the oocyte oxygenation, which could potentially be used as an indicator for selecting high quality oocytes for producing high quality embryos. This could ultimately contribute to improvement of the success rates of human In-Vitro Fertilisation. In light of this issue, this work developed a method for measuring the oxygen consumption rate of granulosa cells in vitro. This included techniques related to granulosa cell harvest from cows, suspending/culturing granulosa cells in culture medium and development of a competent respirometer. Each measurement run on the oxygen consumption rate of granulosa cells was conducted by suspending the granulosa cell culture in the respirometer, in which an optical-based oxygen sensor probe was employed to continuously monitor the oxygen partial pressure change in the cell suspension. Five separate sets of respirometer data were collected and used to calculate the oxygen consumption rate, giving a range of 2.1 to 3.3 x 10-16mol.cell-1.s-1/0.16 to 0.25mol.m-3.s-1. These rates were comparable with but higher than other animal cell oxygen consumption rates reported by the literature. They were approximately 5 times higher than the oxygen consumption rate of granulosa cells harvested from sheep (Gosden & Byatt-Smith 1986). The implications of the measured oxygen consumption rate were then examined in the context of oxygen transport in large bovine preantral follicles via an existing mathematical model. The resulting predicted oxygen profiles in large bovine follicles were consistent with the study of Redding et al. (2007), which showed that as a preantral follicle grew the oxygen transport across the follicle was increasingly strained, resulting in subsequent decrease in oocyte oxygenation. By applying the bovine specific parameter estimates to the model, this work predicted that the largest follicle radius for the oxygen transport in bovine preantral follicle was 134μm, beyond which the oxygen could not reach oocyte. Since the experimentally reported sizes of the large bovine preantral follicles ranged of 58 to 145μm in radius (see Section 7.1.2), this work proposed that the oxygen transport was capable of oxygenating the oocytes in all but the largest preantral follicles. If bovine preantral follicles were to grow larger than they are experimentally observed to do so, all oocytes contained within such follicles would be in a hypoxic state. This is a result consistent with other work. Furthermore, based on the use of bovine and ovine specific parameter estimates in the model, this work found that oxygen transport in follicles was likely to be the result of a unique combination of parameters for a particular species.…

Subjects/Keywords: Oxygen consumption; Granulosa cells; Oocytes; Oxygen consumption measurement; Bovine follicles; Oocyte oxygenation; Preantral follicles

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, D. (2012). Oxygen consumption of bovine granulosa cells in vitro. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/3986

Chicago Manual of Style (16^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Masters Thesis, Massey University. Accessed March 08, 2021. http://hdl.handle.net/10179/3986.

MLA Handbook (7^th Edition):

Li, Dongxing. “Oxygen consumption of bovine granulosa cells in vitro.” 2012. Web. 08 Mar 2021.

Vancouver:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Internet] [Masters thesis]. Massey University; 2012. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10179/3986.

Council of Science Editors:

Li D. Oxygen consumption of bovine granulosa cells in vitro. [Masters Thesis]. Massey University; 2012. Available from: http://hdl.handle.net/10179/3986

15. Santana, Laura Ferreira. Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina.

Degree: PhD, Ginecologia e Obstetrícia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-26092013-155217/ ;

► OBJETIVO: avaliação da expressão gênicado receptor do fator de crescimento semelhante à insulina de (Insulin-Like Growth Factor- IGF) 1 em células da granulosa luteinizadas do… (more)

▼ OBJETIVO: avaliação da expressão gênicado receptor do fator de crescimento semelhante à insulina de (Insulin-Like Growth Factor- IGF) 1 em células da granulosa luteinizadas do cumulusde mulheres não obesas, com sensibilidade à insulina normal, com síndrome dos ovários policísticos (SOP) tratadas e não tratadas com metformina. MODELO DO ESTUDO: prospectivo, longitudinal, randomizado. PACIENTES E MÉTODOS: avaliamos 12 mulheres com ciclosovulatórios, 9 mulheres com SOP e 8 mulheres com SOP e tratadas com metformina, ao menos 8 semanas na dose de 1.700 mg/dia. Todos os grupos foram similares com relação ao peso, ao índice de massa corporal (IMC), à circunferência da cintura e com sensibilidade à insulina normal. Todas as mulheres foram submetidas à estimulação ovariana controlada com uso de agonista de GnRH em protocolo longo e gonadotrofinas para ciclos de FIV/ICSI. As células da granulosa do cumulusforam obtidas por microdissecção dos cinco maiores folículos pré-ovulatórios. A expressão gênica do receptor de IGF-1 foi determinada com técnica da Reação da Polimerase em Cadeia a partir da Transcrição Reversa (Reverse transcriptase - Polymerase Chain ReactionRT-PCR) emiquantitativa. Foram avaliadas as concentrações séricas e foliculares de estradiol, progesterona, testosterona, hormônio folículo estimulante (Follicle-Stimulating Hormone- FSH), hormônio luteinizante (Luteinizing Hormone - LH), Sex Hormone-Binding Globulin(SHBG), glicose, insulina e IGF-1. Para análise estatística, foram utilizados os testes: ANOVA, Newman-Keuls, coeficiente de Pearsone regressão linear múltipla, sendo considerado nível de significância de 5%. RESULTADOS: não foram observadas diferenças com relação à expressão gênica do receptor de IGF-1 nos três grupos analisados (P>0,05). O número de oócitos (20,4 vs. 13,1 vs.11,5, P= 0,009), os níveis séricos de estradiol (1.896,00 pcg/mL vs. 985,20 pcg/mL vs.908,10 pcg/mL,P = 0,03) e testosterona (1,43 ng/mL vs.0,89 ng/mL vs. 0,82 ng/mL pcg/mL,P = 0,02) foram maiores no grupo de mulheres com SOP não tratadas com metformina em comparação com as mulheres com ciclos ovulatórios e tratadas com metformina, respectivamente. As mulheres com ciclos ovulatórios (50.710±42.520 ng/mL) apresentaram maiores concentrações foliculares de progesterona quando comparados com as mulheres com SOP tratadas (13.660±5.212 ng/mL) e não tratadas com metformina (17.680±6.644 ng/mL) (P=0,01). Na avaliação da regressão múltipla, a testosterona sérica não sofreu influência da expressão gênica do receptor de IGF-1 ou do IMC. CONCLUSÕES: as altas concentrações séricas de estradiol e testosterona, maior número de oócitos no grupo de mulheres com SOP não tratadas com metformina nos levam a concluir que mulheres com SOP provavelmente têm uma maior sensibilidade à estimulação da esteroidogênese ovariana quando comparadas com mulheres sem essa doença, embora não tenhasido encontrada diferença na expressão do receptor de IGF-1 nos trêsgrupos analisados. A similaridade dos resultados deste estudo entre mulheres com… Advisors/Committee Members: Reis, Rosana Maria dos.

Subjects/Keywords: assisted reproduct; Células da granulosa.; Expressão gênica; Gene expression; granulosa cells; IGF-1 receptor; metformin; Metformina; PCOS; Receptor de IGF-1; SOP

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Santana, L. F. (2007). Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17145/tde-26092013-155217/ ;

Chicago Manual of Style (16^th Edition):

Santana, Laura Ferreira. “Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina.” 2007. Doctoral Dissertation, University of São Paulo. Accessed March 08, 2021. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-26092013-155217/ ;.

MLA Handbook (7^th Edition):

Santana, Laura Ferreira. “Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina.” 2007. Web. 08 Mar 2021.

Vancouver:

Santana LF. Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Mar 08]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-26092013-155217/ ;.

Council of Science Editors:

Santana LF. Expressão gênica do receptor de IGF-1 em células da granulosa luteinizadas de mulheres com síndrome dos ovários policísticos (SOP), não obesas, com sensibilidade à insulina normal, tratadas e não tratadas com metformina. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-26092013-155217/ ;

16. Abreu, Lauriane Giselle de. Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida.

Degree: PhD, Ginecologia e Obstetrícia, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/ ;

►

Introdução:Até 60% das mulheres com endometriose apresentam como sintoma a infertilidade. Entretanto, os mecanismos envolvidos ainda permanecem não totalmente esclarecidos, especialmente quando não há distorção… (more)

▼

Introdução:Até 60% das mulheres com endometriose apresentam como sintoma a infertilidade. Entretanto, os mecanismos envolvidos ainda permanecem não totalmente esclarecidos, especialmente quando não há distorção da anatomia pélvica. A etiologia multifatorial e comprometimento poligênico nesta doença têm sido amplamente aceitos. A aromatase é uma molécula das mais estudadas e há evidências de aumento da expressão do seu gene no endométrio eutópico e ectópico na endometriose. Esta enzima, codificada pelo gene CYP19A1, converte andrógenos a estrógenos e está presente normalmente nas células da granulosa, onde é fundamental para a produção esteroidogênica intrafolicular. Estudos in vitropor cultivo de células da granulosa, mostraram redução da atividade da aromatase em mulheres com endometriose. Devido à escassez de estudos que analisem a expressão do seu gene (CYP19A1) nessas células foi o que estimulou a proposta deste estudo. Este trabalho tem porobjetivo medir a expressão do gene da aromatase por PCR em tempo real nas células da granulosa luteinizadas murais de mulheres com endometriose submetidas a técnicas de reprodução assistida. Pacientes e Métodos: Estudo caso-controle, com 11 mulheres com endometriose e 11 com os fatores tubáreo ou masculino de infertilidade,submetidas à hiperestimulação ovariana controlada (HOC) para reprodução assistida, num total de 12 ciclos para o grupo com endometriose e 11 para o controle. Não houve diferença entre as características clínicas dos dois grupos quanto à idade e parâmetros do ciclo de HOC. As células da granulosa murais foram coletadas de folículos pré-ovulatórios maduros no dia da captação oocitária e isoladas. Posteriormente, procedeu-se à extração do RNA (clorofórmio/ isopropanol) e à transcrição reversa. A PCR em tempo real foi realizada para quantificar os níveis de RNA mensageiro produzidos para o gene da aromatase, normalizados aos produtos do gene endógeno, ß-actina (expressão relativa). Todos os experimentos foram realizados em duplicata. Resultados: não houve diferença na expressão do gene CYP19A1 nas células da granulosa luteinizadas murais de mulheres com endometriose quando comparadas ao grupo controle (p>0,05; Mann Whitney), mesmo na comparação combinada considerando-se separadamente os diferentes graus de endometriose (controle vs. endometriose mínima/leve vs. endometriose moderada/grave, p>0,05;Kruskall Wallis). Conclusão:Os resultados deste estudo sugerem que a aromatase apresenta um mecanismo complexo de controle para sua expressão gênica nas células da granulosa e, apesar de evidências prévias de sua reduzida atividade nessas células na endometriose, a expressão de seu gene parece não estar afetada pela doeça, de acordo com o presente estudo.

Background: Up to 60% of women with endometriosis have infertility symptoms. However, mechanisms remain unclear, mainly when there is no distortion of pelvic anatomy. The multifactorial etiology and polygenic involvement of this disease have been widely accepted.…

Advisors/Committee Members: Sá, Marcos Felipe Silva de.

Subjects/Keywords: Aromatase; Aromatase; Assisted reproduction; Células da granulosa; CYP19A1; CYP19A1; Endometriose; Endometriosis; Granulosa cells; Infertilidade; Infertility; PCR em tempo real; Real-time PCR; Reprodução assistida

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abreu, L. G. d. (2009). Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/ ;

Chicago Manual of Style (16^th Edition):

Abreu, Lauriane Giselle de. “Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida.” 2009. Doctoral Dissertation, University of São Paulo. Accessed March 08, 2021. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/ ;.

MLA Handbook (7^th Edition):

Abreu, Lauriane Giselle de. “Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida.” 2009. Web. 08 Mar 2021.

Vancouver:

Abreu LGd. Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Mar 08]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/ ;.

Council of Science Editors:

Abreu LGd. Expressão do gene da aromatase (CYP19A1) nas células da granulosa murais luteinizadas de mulheres com endometriose submetidas a técnicas de reprodução assistida. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27092013-094914/ ;

Universidade Federal de Santa Maria

17. Valério Valdetar Marques Portela Junior. REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS.

Degree: 2007, Universidade Federal de Santa Maria

URL: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1424

► O objetivo deste trabalho foi estabelecer o controle de expressão dos receptores de angiotencina II (AngII) e determinar a ação fisiológica da AngII em células… (more)

▼ O objetivo deste trabalho foi estabelecer o controle de expressão dos receptores de angiotencina II (AngII) e determinar a ação fisiológica da AngII em células da granulosa (CG) cultivadas in vitro. Os receptores de AngII tipo 1 (AGTR1) e tipo 2 (AGTR2) foram localizados em folículos de bovinos de diferentes tamanhos. Verificouse que as CG de bovinos provenientes de folículos entre 2- 5 mm e cultivadas com FSH, IGF-1, BMP-7 apresentaram aumento na expressão do receptor AGTR2 (P<0,05) em relação ao grupo controle (GC), bem como aumento da secreção de estradiol (E2; P<0,05). Em contraste, as CG tratadas com 10 ng/ml de FGF-2 ou 10 e 100 ng/ml de FGF-7 e FGF-10 apresentaram uma redução na secreção de E2 (P<0,05), porém somente os grupos FGF-7 e 10 nas doses 10 e 100 ng/ml reduziram (P<0,05) a expressão do receptor AGTR2. Para os dois experimentos, não houve diferença na expressão do receptor AGTR1 entre o GC e os grupos tratados. As CG e células da teca (CT) foram coletadas de ovários provenientes de abatedouro para extração de RNA e o fluido folicular para dosagem de E2 e progesterona P4. Esses folículos foram classificados como dominantes (FD; P4<100ng/ml e E2>100ng/ml) e atrésicos (FA; P4>40ng/ml). A expressão dos receptores AGTR1 e AGTR2 foi mensurada por RTPCR. Não houve diferença na expressão do receptor AGTR1 entre FD e FA. No entanto, o receptor AGTR2 apresentou um aumento (P<0,05) na expressão em CG de FD em relação a FA. A expressão do receptor AGTR1 se manteve constante em CG e CT de FD e FA. Para determinar os afeitos da AngII através da ativação de seus receptores CG foram cultivadas em meio livre de soro com FSH e/ou AngII. A AngII não apresentou efeito na secreção de E2 ou P4, mas inibiu (P<0.05) mRNA e proteína para a protease nexin-1 (PN-1). Considerando a redução de expressão da PN-1 envolvida no controle do remodelamento da matriz extracelular (RME), é possível especular um efeito de AngII sobre RME durante o desenvolvimento folicular. Em um terceiro experimento, as CG de folículos grandes (>10mm) foram cultivadas durante 6h, 12h e 24h na presença de Ang (10-5) com ou sem LH (100ng/ml). A combinação de AngII e LH aumentou significativamente (P<0,05) a expressão de mRNA e proteína para COX-2, ativador do plasminogênio tipo U e T, bem como para PN-1. Entretanto, AngII ou LH não aumentaram a expressão de COX-2. O aumento da expressão destes gene indica uma função de AngII no processo de ovulação através das CG. Em um segundo momento, verificou-se através de qual receptor a AngII atua para controlar a expressão desses genes. As CG foram cultivadas por 6h com LH e/ou AngII com ou sem inibidores específicos para o receptor AGTR1 (losartan) e AGTR2 (PD123,319). Os resultados demonstraram que a presença do inibidor de AGTR2 bloqueou o efeito da associação de LH e AngII em relação ao grupo controle (P<0.05), demonstrado que a ação da AngII é mediada pelo receptor AGTR2 em CG. Em conclusão, o receptor AGTR2 está presente nas células da granulosa de bovinos e o mRNA para o receptor AGTR2 é regulado durante o… Advisors/Committee Members: Paulo Bayard Dias Goncalves, Jose Carlos Ferrugem Moraes, José Buratini Junior, Danila Barreiro Campos, Magda Vieira Benavides.

Subjects/Keywords: remodelamento da matriz extra celular; angiotensina II; desenvolvimento folicular; células da granulosa; MEDICINA VETERINARIA; angiotensin II; matrix extracelular remodeling; follicular development; granulosa cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Junior, V. V. M. P. (2007). REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS. (Thesis). Universidade Federal de Santa Maria. Retrieved from http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1424

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Junior, Valério Valdetar Marques Portela. “REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS.” 2007. Thesis, Universidade Federal de Santa Maria. Accessed March 08, 2021. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1424.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Junior, Valério Valdetar Marques Portela. “REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS.” 2007. Web. 08 Mar 2021.

Vancouver:

Junior VVMP. REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS. [Internet] [Thesis]. Universidade Federal de Santa Maria; 2007. [cited 2021 Mar 08]. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1424.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Junior VVMP. REGULAÇÃO DA EXPRESSÃO DO RECEPTOR AT2 E EFEITO DA ANGIOTENSINA II SOBRE A EXPRESSÃO DE GENES ENVOLVIDOS NO DESENVOLVIMENTO FOLICULAR E OVULAÇÃO EM CÉLULAS DA GRANULOSA DE BOVINOS. [Thesis]. Universidade Federal de Santa Maria; 2007. Available from: http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=1424

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

18. Sèdes, Lauriane. Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract.

Degree: Docteur es, Biologie, 2014, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2014PA11T014

► L’hormone anti-Müllerienne (AMH) est un membre de la famille TGF-β impliquée dans la différenciation du tractus reproductif mâle. Elle est aussi exprimée par les cellules… (more)

▼ L’hormone anti-Müllerienne (AMH) est un membre de la famille TGF-β impliquée dans la différenciation du tractus reproductif mâle. Elle est aussi exprimée par les cellules de la granulosa de l’ovaire adulte. Cependant, son rôle physiologique chez la femelle n’a pas encore été entièrement établi. Mon projet de thèse a pour objectif d’élucider le(s) rôle(s) de l’AMH dans le tractus reproductif femelle. L’AMH transduit ses effets par l’intermédiaire de deux récepteurs transmembranaires sérine/thréonine kinase : un récepteur de type II qui lui est spécifique (AMHR-II) et un récepteur de type I (ActR-IA, BMPR-IA, BMPR-IB) qu’elle partage avec les BMPs. Après fixation de l’hormone sur le récepteur de type II, celui-ci recrute et phosphoryle le récepteur de type I. Ce dernier phosphoryle à son tour les Smads spécifiques (Smad1, 5 et 8) qui s’associent à la Smad commune, Smad4. L’ensemble transloque dans le noyau et en association avec des facteurs de transcription régule les gènes cibles de l’hormone. L'utilisation de souris KO conditionnelles pour les récepteurs Acvr1 et Bmpr1a et d'une technique de siRNA dirigés contre chacun des trois récepteurs de type I a permis de mettre en évidence que le récepteur BMPR-IA est un acteur essentiel de la voie de signalisation de l'AMH dans les cellules de la granulosa. Pour déterminer la ou les Smad(s) impliquées, une technique de gènes rapporteurs, Smad-Gal4/UAS-luciférase, a été utilisée. Nous avons pu montrer que les Smad1 et 5 sont importantes pour la transduction du signal de l'AMH dans les cellules de la granulosa. Récemment des corécepteurs aux BMPs, les Repulsive Guidance Molecule (RGMs), ont été mis en évidence. L’AMH partageant sa voie de signalisation avec les BMPs, nous avons cherché à déterminer si ces corécepteurs pouvaient également intervenir dans la voie de signalisation de l’AMH. Il existe 3 types de RGMs: RGMa, RGMb et RGMc. Nous avons montré en q-PCR que seul RGMb est exprimé dans les cellules de la granulosa alors que les 3 RGMs sont exprimés dans l’ovaire. L'utilisation de siRNA dirigés contre RGMb a permis de montrer que ce récepteur n'est pas nécessaire à la transduction du signal de l'AMH. Actuellement, seuls deux gènes cibles de l’AMH sont connus dans les cellules de la granulosa : l’aromatase et le récepteur LH. Nous avons réalisé des analyses de puces à ADN (ou micro-array) pour décrire de nouveaux gènes cibles de l'AMH. L'analyse des puces a permis de décrire de nouveaux gènes régulés par cette hormone tels qu’Ovgp1 ou Kcnj2. La dernière partie de mon projet visait à déterminer un rôle potentiel de l'AMH dans l'utérus. En effet, le récepteur de cette hormone est exprimé dans le myomètre utérin de souris permettant de supposer qu’elle peut agir sur cet organe. Nous avons pu mettre en évidence une expression faible du gène de l’Amh dans l’utérus. En revanche, l’expression et la localisation de la protéine restent encore à définir. Une expérience de PCR-array a permis de montrer que de nombreux gènes sont différentiellement exprimés entre l’utérus Wt et… Advisors/Committee Members: Jamin, Soazik (thesis director).

Subjects/Keywords: Hormone anti-Müllerienne; Ovaire; Utérus; Cellules de la granulosa; Signalisation; Gènes cibles; Anti-Müllerian hormone; Ovary; Uterus; Granulosa cells; Signalisation; Target genes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sèdes, L. (2014). Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2014PA11T014

Chicago Manual of Style (16^th Edition):

Sèdes, Lauriane. “Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract.” 2014. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 08, 2021. http://www.theses.fr/2014PA11T014.

MLA Handbook (7^th Edition):

Sèdes, Lauriane. “Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract.” 2014. Web. 08 Mar 2021.

Vancouver:

Sèdes L. Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2014. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2014PA11T014.

Council of Science Editors:

Sèdes L. Voie de signalisation et gènes cibles de l’AMH dans le tractus génital femelle : AMH signaling pathway and its target genes in female reproductive tract. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2014. Available from: http://www.theses.fr/2014PA11T014

19. M. Albonico. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.

Degree: 2017, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/489075

► Mycotoxins, secondary metabolites produced by moulds, mainly Aspergillus spp., Fusarium spp. and Penicillium spp., are common contaminants of food and feed. The aim of this… (more)

▼ Mycotoxins, secondary metabolites produced by moulds, mainly Aspergillus spp., Fusarium spp. and Penicillium spp., are common contaminants of food and feed. The aim of this project was to evaluate: (i) the potential endocrine disruptor effects of fumonisin B1 (FB1), beauvericin (BEA), deoxynivalenol (DON) and zearalenone (ZEA) metabolites �-zearalenol (�-ZEA) and �zearalenol (�ZEA), alone and combined, using a bovine granulosa cell (GC) in vitro model and (ii) the individual and combined effects of FB1 and BEA on the intestinal barrier using Caco-2 cells cultured in vitro on semipermeable inserts. The results obtained indicated that FB1 alone at all tested doses (0; 0.5; 1; 1.5; 3; 6 �M) had no effects on GC proliferation and progesterone (P4) production. In the presence of �ZEA at30 ng/mL (0.094 �M), FB1 at 30 ng/mL (0.042 �M) showed a stimulatory effect on GC numbers. Cell proliferation decreased after exposure to �ZEA alone at 5.0 mg/mL (15.6 �M) and FB1 with �-ZEA and �ZEA at the same concentration. Regarding steroid production, FB1 at 30 ng/mL (0.042 �M ) and 100 ng/mL (0.13 �M amplified the inhibitory effect of �ZEA at 30 ng/mL (0.094 �M) on estradiol (E2) production, while FB1 alone increased (P<0.05) IGF1-induced E2 production. FB1 in combination with �ZEA decreased (P < 0.05) E2 production. FB1 at 1, 1.5 and 3 �M slightly inhibited (P < 0.05) E2 production. BEA at concentrations � 3 �M was found to strongly decrease (P < 0.05) both steroid production and FB1 did not influence the effects of BEA. At 10 �M both mycotoxins decreased (P < 0.001) serum-induced GC proliferation. At 30 �M, BEA showed inhibitory effects on FSH plus IGF-1-induced CYP11A1 and CYP19A1 mRNA abundance (P < 0.05), whereas FB1 at 30 �M had no effect on CYP11A1 and CYP19A1 gene expression. As regards the effects of FB1 and BEA, alone and combined, on the Caco-2 intestinal barrier model data showed a TEER decrease after 1 h and 2 h of Bl exposure to BEA at 0.5 and 1.5 �M and after 24 h of Bl exposure to BEA at 0.5 �M, whereas after 24 h of Bl exposure, BEA at 3 and 6 �M was found to significantly (P < 0.05) increase TEER. FB1 had no effect on the intestinal barrier integrity and when combined with BEA the TEER increase induced by BEA was no longer observed. Cytokine release was observed only after exposure to BEA alone, and not in combination with FB1, with an increase of IL-6 and IL8 release after apical exposure to 3 and 6 �M and after basolateral exposure to 1.5, 3, 6 �M for IL-6 and only to 6 �M for IL-8. TNF- � release was induced by Ap (0.5 -1.5 �M) and Bl (1.5 �M) exposure to BEA. Overall, these results provide information on in vitro toxicological effects of Fusarium mycotoxins. Advisors/Committee Members: tutor: F. Caloni, coordinatore: F. Gandolfi, CALONI, FRANCESCA, GANDOLFI, FULVIO.

Subjects/Keywords: Mycotoxins; Fusarium; granulosa cells; Caco-2 cells; fumonisin; beauvericin; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Albonico, M. (2017). IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/489075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Albonico, M.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.” 2017. Thesis, Università degli Studi di Milano. Accessed March 08, 2021. http://hdl.handle.net/2434/489075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Albonico, M.. “IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS.” 2017. Web. 08 Mar 2021.

Vancouver:

Albonico M. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. [Internet] [Thesis]. Università degli Studi di Milano; 2017. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2434/489075.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Albonico M. IN VITRO TOXICOLOGICAL EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH OTHER MYCOTOXINS. [Thesis]. Università degli Studi di Milano; 2017. Available from: http://hdl.handle.net/2434/489075

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Bothun, Alisha. Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation.

Degree: PhD, Department of Biology, 2019, Northeastern University

URL: http://hdl.handle.net/2047/D20316319

► The mammalian ovary performs two primary functions: the production of germ cells and the release of steroid hormones. The ovarian cortex contains many follicles, each… (more)

▼ The mammalian ovary performs two primary functions: the production of germ cells and the release of steroid hormones. The ovarian cortex contains many follicles, each composed of a single oocyte surrounded by somatic support cells; these somatic support cells consist of granulosa and theca cells, which become steroidogenic as the follicle grows. These cell types together form the basis for human oocyte support and maturation, as well as participate in the hypothalamic-pituitary-gonadal (HPG) axis to maintain hormone production and signaling. During embryonic development, ovarian follicles are formed when migrating mitotic primordial germ cells (PGCs) reach the gonad, associate with a layer of pre-granulosa cells, initiate meiosis, and subsequently remain in meiotic arrest until sexual maturity. Upon follicle activation, the pre-granulosa cells proliferate and begin hormone generation, the follicle grows in size, and the oocyte is ovulated for possible fertilization. Because follicular assembly and cell specification is established during embryonic development, the signals governing these transitions are not fully elucidated and only recently tools have become available to study these complex cellular differentiation stages and interactions. Through the use of a quantitative proteomics analysis of the human ovary during the first and second trimesters of development, I established a baseline protein expression profile during the establishment of the ovary when PGCs are newly differentiating into oogonia through the stages of follicle formation and into adulthood. This screen allowed me to validate markers of germ cells not previously identified in human cells such as MAEL and TEX11 and show that they could be used in stem cell models of in vitro germ cell differentiation. Additionally, I provide evidence for the in vitro differentiation of granulosa cells from human pluripotent stem cells. This global protein expression profile also enabled me to further probe signaling cascades that have not previously been investigated in human folliculogenesis, including the finding for support of canonical WNT signaling in the oocyte at the time of follicle assembly; it also allowed me to investigate the transition of metabolism and metabolic control and identify that the fetal ovary is highly reliant on mitochondrial activity during PGC differentiation and follicle formation. To highlight the role of mitochondria in germ cell differentiation, I further utilized newly validated techniques for germ cell differentiation from pluripotent stem cells; by carefully tracking mitochondrial activity during the initial stages of PGC differentiation I found that the mitochondrial activity of the precursor stem cells to PGCs influences differentiation, and that stem cells that are more slowly proliferating have less active mitochondria yet preferentially differentiate into PGCs in vitro. The compilation of a well-defined source of stem cell derived oocytes and in vitro derived granulosa cells will allow the observation of human follicle…

Subjects/Keywords: Germ cell differentiation; Granulosa cells; Mitochondria; Ovarian development; Pluripotent stem cells; Cellular biology; Biology; Developmental biology

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bothun, A. (2019). Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20316319

Chicago Manual of Style (16^th Edition):

Bothun, Alisha. “Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation.” 2019. Doctoral Dissertation, Northeastern University. Accessed March 08, 2021. http://hdl.handle.net/2047/D20316319.

MLA Handbook (7^th Edition):

Bothun, Alisha. “Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation.” 2019. Web. 08 Mar 2021.

Vancouver:

Bothun A. Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation. [Internet] [Doctoral dissertation]. Northeastern University; 2019. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2047/D20316319.

Council of Science Editors:

Bothun A. Characterization Of Human Oocyte Development And The Role Of Mitochondrial Activity In In Vitro Germ Cell Differentiation. [Doctoral Dissertation]. Northeastern University; 2019. Available from: http://hdl.handle.net/2047/D20316319

21. Chandrasekher, Yasmin Aladin. Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells.

Degree: PhD, 1993, Oregon Health Sciences University

URL: doi:10.6083/M447483N ; http://digitalcommons.ohsu.edu/etd/2855

Subjects/Keywords: Gonadotropins; Luteinizing Hormone; Receptors, Progesterone; Macaca mulatta; Granulosa Cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chandrasekher, Y. A. (1993). Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M447483N ; http://digitalcommons.ohsu.edu/etd/2855

Chicago Manual of Style (16^th Edition):

Chandrasekher, Yasmin Aladin. “Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells.” 1993. Doctoral Dissertation, Oregon Health Sciences University. Accessed March 08, 2021. doi:10.6083/M447483N ; http://digitalcommons.ohsu.edu/etd/2855.

MLA Handbook (7^th Edition):

Chandrasekher, Yasmin Aladin. “Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells.” 1993. Web. 08 Mar 2021.

Vancouver:

Chandrasekher YA. Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 1993. [cited 2021 Mar 08]. Available from: doi:10.6083/M447483N ; http://digitalcommons.ohsu.edu/etd/2855.

Council of Science Editors:

Chandrasekher YA. Novel action of the gonadotropin surge in rhesus monkeys : expression of progesterone receptors in granulosa cells. [Doctoral Dissertation]. Oregon Health Sciences University; 1993. Available from: doi:10.6083/M447483N ; http://digitalcommons.ohsu.edu/etd/2855

22. C. Cortinovis. IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/217456

► Fumonisins, zearalenone and trichothecenes, such as deoxynivalenol (DON) and T-2 toxin, are the major Fusarium mycotoxins occurring throughout the world in cereal grains and animal… (more)

▼ Fumonisins, zearalenone and trichothecenes, such as deoxynivalenol (DON) and T-2 toxin, are the major Fusarium mycotoxins occurring throughout the world in cereal grains and animal feeds. Direct ovarian effects of fumonisin B1 (FB1) and its interaction with DON or α-zearalenol (α-ZOL), zearalenone major active metabolite, have so far not been investigated. Thus, the goal of this thesis was to determine if FB1, alone or combined with DON or α-ZOL, can impair swine reproductive activity via affecting granulosa cell function. To this aim, two different studies were designed. In the first study the effects of FB1 alone and combined with DON or α-ZOL on granulosa cell proliferation were evaluated. Porcine granulosa cells from small ovarian follicles (1-5 mm) were cultured for 2 days in 5% fetal bovine serum and 5% porcine serum-containing medium followed by 2 days in serum-free medium containing FB1 at various doses (0, 0.01, 0.4, 10 and 14 μM) and combinations. At the end of the experiments, numbers of granulosa cells were determined using a Coulter counter. The results revealed that FB1 had inhibitory effects on granulosa cell proliferation at doses ≥ 10 μM. DON (3.4 μM) strongly inhibited (by 80%; P < 0.0001) granulosa cell proliferation and no significant difference was detected in combination with FB1 (10 μM). α-ZOL (9.4 μM) showed a stimulatory effect (P < 0.01) on granulosa cell numbers, even when treated in combination with FB1 (10 μM). In the second study the effects of FB1 alone and combined with DON or α-ZOL on granulosa cell steroid production and gene expression were investigated. Porcine granulosa cells from small follicles (1-5 mm) were cultured as described above. At the end of the experiments, concentrations of progesterone and estradiol in culture medium were determined by radioimmunoassays. Real-time RT-PCR was used to elucidate the effects of FB1 on gene expression of P450scc (CYP11A1) and aromatase (CYP19A1). All doses of FB1 (i.e., 0.01, 0.4, 10 and 14 µM) had no significant effect on estradiol production, whereas FB1 showed a stimulatory effect on progesterone production induced by FSH plus insulin-like growth factor-I (IGF-I) at 10 and 14 µM. α-ZOL (9.4 µM) increased (P < 0.0001) FSH plus IGF-I-induced progesterone production by 51%. Combination of FB1 with α-ZOL resulted in an increase of progesterone production (91%; P < 0.0001) that was significantly higher than that induced by either Fusarium mycotoxin alone. DON drastically inhibited (by 74%; P < 0.0001) progesterone production and FB1 had little effect on this response. α-ZOL had no effect on FSH plus IGF-I-induced estradiol production, whereas decreased (P < 0.05) estradiol production when co-treated with FB1. DON (3.4 µM) strongly inhibited (by 67%; P < 0.0001) estradiol production and no difference was detected in combination with FB1 (10 µM). FB1 (10 µM) had no effect on granulosa cell CYP19A1 mRNA abundance, whereas decreased (by 23%; P < 0.0001) granulosa cell CYP11A1 mRNA abundance induced by FSH plus IGF-I. In conclusion, the… Advisors/Committee Members: tutor: F. Caloni, coordinatore: G. Savoini, CALONI, FRANCESCA, SAVOINI, GIOVANNI.

Subjects/Keywords: fumonisin B1; Fusarium mycotoxins; Granulosa cells; proliferation; steroidogenesis; swine; Settore VET/07 - Farmacologia e Tossicologia Veterinaria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cortinovis, C. (2013). IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/217456

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cortinovis, C.. “IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS.” 2013. Thesis, Università degli Studi di Milano. Accessed March 08, 2021. http://hdl.handle.net/2434/217456.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cortinovis, C.. “IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS.” 2013. Web. 08 Mar 2021.

Vancouver:

Cortinovis C. IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2434/217456.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cortinovis C. IN VITRO EFFECTS OF FUMONISIN B1 ALONE AND COMBINED WITH DEOXYNIVALENOL OR ZEARALENONE ON PORCINE GRANULOSA CELL PROLIFERATION AND STEROIDOGENESIS. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/217456

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

23. Xu, Yafei. Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum .

Degree: 2017, University of Arizona

URL: http://hdl.handle.net/10150/625703

► The mechanisms causing luteolysis of the primate corpus luteum are unknown. There is an increase in expression of liver x receptor (LXR) target genes and… (more)

▼ The mechanisms causing luteolysis of the primate corpus luteum are unknown. There is an increase in expression of liver x receptor (LXR) target genes and reduced low density lipoprotein receptor (LDLR) during spontaneous luteolysis in primates. The LXRs belong to the nuclear receptor superfamily and increase cholesterol efflux by inducing transcription of their target genes. Uptake of cholesterol into primate luteal cells occurs primarily via LDL, and LDLR transcription is regulated by sterol regulatory element binding transcription factor 2 (SREBF2). Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) maintain luteal function by binding to the LH/CG receptor (LHCGR), which stimulates progesterone (P4) synthesis via protein kinase A (PKA). It has also been previously reported that there is an increase in 27-hydroxycholesterol (27OH) concentrations during spontaneous luteolysis in primates. Pregnenolone and P4 inhibit the enzyme activity of CYP27A1 (cytochrome p450, family 27, subfamily A, polypeptide 1), which converts cholesterol into 27OH, an oxysterol that is a natural LXR agonist and SREBF2 inhibitor. Therefore, the overall hypothesis is that LXR-induced cholesterol efflux and reduced LDL uptake via inhibition of SREBF2 activity mediate luteolysis of the human CL. The objective of study 1 is to determine the effects of LXR activation and SREBF2 inhibition on P4 production, cholesterol metabolism and gene expression; and how hCG signaling via PKA regulates these effects in human luteinized granulosa cells. Basal and hCG-stimulated P4 secretion were significantly decreased by the combined actions of the LXR agonist T0901317 (T09) and the SREBF2 inhibitor fatostatin, which was associated with alterations in cholesterol metabolism leading to reduced intracellular cholesterol storage. Expression of LXR target genes in the presence of T09 was significantly reduced by hCG, while hCG significantly increased LDLR expression. These effects of hCG were reversed by a specific PKA inhibitor. Chronic hCG exposure had similar effects on LXR target gene and LDLR expression without an exogenous LXR agonist. The objective of study 2 is to determine the effects of 27OH on P4 production and cholesterol metabolism; and to determine if inhibiting the conversion of cholesterol into pregnenolone increases LXR and decreases SREBF2 target gene expression via CYP27A1 in human luteinized granulosa cells. During luteolysis in primates and sheep, CYP27A1 expression significantly increased. 27OH significantly decreased hCG-stimulated P4 secretion and enhanced cholesterol efflux. Aminoglutethimide, which inhibits the conversion of cholesterol to pregnenolone, significantly increased ABCA1 and decreased LDLR. Knock-down of CYP27A1 resulted in a significant increase in P4 secretion, but did not prevent aminoglutethimide-induced effects on ABCA1 and LDLR. Knock-down of steroidogenic acute regulatory protein (STAR), which controls cholesterol transport into the mitochondria where CYP27A1 resides, significantly decreased LDLR… Advisors/Committee Members: Bogan, Randy L (advisor), Bogan, Randy L. (committeemember), Limesand, Sean W. (committeemember), Hernandez, Jose J. (committeemember).

Subjects/Keywords: 27-hydroxycholestrol; human chorionic gonadotropin; Human luteinized granulosa cells; Liver x receptor; Luteolysis; Sterol regulatory element binding transcription factor 2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, Y. (2017). Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/625703

Chicago Manual of Style (16^th Edition):

Xu, Yafei. “Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum .” 2017. Masters Thesis, University of Arizona. Accessed March 08, 2021. http://hdl.handle.net/10150/625703.

MLA Handbook (7^th Edition):

Xu, Yafei. “Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum .” 2017. Web. 08 Mar 2021.

Vancouver:

Xu Y. Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum . [Internet] [Masters thesis]. University of Arizona; 2017. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10150/625703.

Council of Science Editors:

Xu Y. Enhanced Liver X Receptor and Decreased Sterol Regulatory Element Binding Transcription Factor 2 Activities May Control Luteolysis of the Human Corpus Luteum . [Masters Thesis]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/625703

University of Kansas

24. Fiedler, Stephanie Deanne. MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS.

Degree: MS, Molecular & Integrative Physiology, 2008, University of Kansas

URL: http://hdl.handle.net/1808/4331

► Normal ovarian function is an important aspect of reproductive health, and loss of this function can have drastic consequences including infertility as well as an… (more)

▼ Normal ovarian function is an important aspect of reproductive health, and loss of this function can have drastic consequences including infertility as well as an increased risk for diseases like ovarian cancer. The ability to initiate and maintain a pregnancy is dependent on the regulation of oocyte developmental competence, ovulation and corpus luteum function as each makes an important contribution to proper ovarian function. During the periovulatory period, ovulation is initiated as a surge of LH induces granulosa cells to rapidly transform from estrogen producing follicular cells to progesterone producing luteal cells. The molecular mechanisms involved with the follicular to luteal transition have been extensively studied, and many transcriptional changes have been associated with steroidogenic and/or cell differentiation pathways. Comparatively, little attention has been given to post-transcriptional regulatory events during this period despite such events having an apparent role in maintaining the integrity of gene expression. MicroRNA (miRNA) are highly conserved, 21nt long RNA molecules that offer a way to regulate gene expression at the post-transcriptional level by binding to the 3'UTR region of a target mRNA and either preventing its translation or causing its degradation. MicroRNA have been shown to be involved in cell differentiation in other systems implying that they may also have a role in the changes that occur within granulosa cells following the LH surge. Within this thesis, I discuss several studies designed to look at miRNA expression in periovulatory granulosa cells. In addition, I describe several methods that can be used to measure levels of miRNA in their mature form as well as techniques used to begin to determine their functional relevance to the processes of ovulation and luteinization. I hypothesize that specific miRNA are induced by the LH surge, and that these miRNA are involved with regulating translation of specific target mRNA(s) within periovulatory granulosa cells. Using a mouse model, I have shown 13 miRNA to be differentially expressed at 4 hours post hCG, a critical time during the periovulatory period when many changes in gene expression are known to occur. MicroRNA-132 and miRNA-212 were found to be two of the most highly upregulated miRNA at this time point; therefore, I chose to narrow our examination to the characterization of these two miRNA and their potential mRNA targets within the periovulatory follicle. In addition to miRNA studies, I have also begun to look at the gene within which miRNA-132 and 212 reside. Experiments designed to look at the expression of this gene suggest that it is regulated by LH as well. The results I present here provide the groundwork necessary for studying the function and regulation of miRNA-132 and 212 within periovulatory follicles. Advisors/Committee Members: Christenson, Lane K. (advisor), Albertini, David F. (cmtemember), Heckert, Leslie L. (cmtemember), Wolfe, Michael W. (cmtemember).

Subjects/Keywords: Molecular biology; Granulosa cells; Luteinization; MicroRNA; Ovulation; Reproduction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fiedler, S. D. (2008). MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS. (Masters Thesis). University of Kansas. Retrieved from http://hdl.handle.net/1808/4331

Chicago Manual of Style (16^th Edition):

Fiedler, Stephanie Deanne. “MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS.” 2008. Masters Thesis, University of Kansas. Accessed March 08, 2021. http://hdl.handle.net/1808/4331.

MLA Handbook (7^th Edition):

Fiedler, Stephanie Deanne. “MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS.” 2008. Web. 08 Mar 2021.

Vancouver:

Fiedler SD. MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS. [Internet] [Masters thesis]. University of Kansas; 2008. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1808/4331.

Council of Science Editors:

Fiedler SD. MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS. [Masters Thesis]. University of Kansas; 2008. Available from: http://hdl.handle.net/1808/4331

25. Metallinou, Chryssoula. In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων.

Degree: 2013, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ)

URL: http://hdl.handle.net/10442/hedi/29362

► The hypothalamic gonadotropin releasing hormone (GnRH) regulates via pituitary high affinity receptors (GnRHR-I) the biosynthesis and secretion of gonadotropins. In addition to its central action… (more)

▼ The hypothalamic gonadotropin releasing hormone (GnRH) regulates via pituitary high affinity receptors (GnRHR-I) the biosynthesis and secretion of gonadotropins. In addition to its central action it has been suggested that GnRH exerts peripheral actions since the presence of GnRHR-I has been demonstrated in many extrapituitary tissues including the human ovary. In particular GnRHR-I mRNA has been detected in human granulosa-lutein cells (hGL). On the other hand, the broad use of GnRH analogues by patients undergoing assisted reproductive techniques in ovarian stimulation protocols has raised concerns regarding their possible direct effects on ovarian functions.In the present study we investigated in vitro the effects of GnRH analogues (the GnRH agonist triptorelin and the GnRH antagonist cetrorelix) on the viability and steroidogenesis of cultured hGL cells. Furthermore, we examined their effects on the secretion of two growth factors: the vascular endothelial growth factor (VEFG) and the basic fibroblast growth factor (bFGF). The hGL cells were collected at the time of oocyte retrieval from 52 women (26-40 years old) who participated in IVF cycles due to male or tubal factor infertility. hGL cells were cultured for 48 h with cetrorelix or triptorelin each at concentrations of 1 nM and 3 nM to serum free media. After treatment, the viability was evaluated with the MTT (3,[4,5-dimethylthiazol-2yl]2,3dipheniltetrazolium bromide) assay. Concentrations of estradiol, progesterone VEGF, bFGF in culture supernatants were measured by ELISA.Further experiments with the previous experimental design were performed with the addition of 1 IU of human chorionic gonadotropin (hCG) in the culture medium in order to stimulate the production of steroid hormones and growth factors.Additionally, we investigated in vitro the effects of two GnRH agonists: triptorelin and buserelin as well the effects of cetrorelix on the viability of HGL-5, a human lutein granulosa cell line. HGL-5 cells were cultured for 48 h and treated with or without GnRH agonist or antagonist, each at concentrations of 1 nM and 3 nM, to serum free media. The cell viability was evaluated by the MTT test. Further, we investigated the mRNA expression of GnRHR-I in HGL-5 cells.ABSTRACTviiTreatment of cultured cells with triptorelin induced a slightly increase on the cell viability whereas treatment with cetrorelix led to a significant decrease of hGL cells viability.The high dose of triptorelin reduced the E2 production of hGL cells compared to cells treated with low dose of cetrorelix. Furthermore, cultures treated with 3 nM cetrorelix or triptorelin tended to secrete lower progesterone levels. Regarding the release of VEGF no statistically significant differences were found between cells treated with GnRH analogues and cells incubated with the culture medium only. Treatment with 1 nM of cetrorelix significantly decreased the bFGF levels against control group. The addition of hCG showed not considerably different results compared with the absence of hCG.GnRH analogues…

Subjects/Keywords: GnRh ανάλογα; Ωχρινοποιημένα κοκκώδη κύτταρα; Βιωσιμότητα; Στεροειδογένεση; VEGF (Aγγειογενετικός ενδοθηλιακός αναπτυξιακός παράγοντας ); bFGF; GnRH analogues; Viability; Granulosa luteinized cells; STEROIDOGENESIS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Metallinou, C. (2013). In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων. (Thesis). Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Retrieved from http://hdl.handle.net/10442/hedi/29362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Metallinou, Chryssoula. “In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων.” 2013. Thesis, Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ). Accessed March 08, 2021. http://hdl.handle.net/10442/hedi/29362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Metallinou, Chryssoula. “In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων.” 2013. Web. 08 Mar 2021.

Vancouver:

Metallinou C. In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων. [Internet] [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10442/hedi/29362.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Metallinou C. In vitro μελέτη της επίδρασης των GNRH ανάλογων επί των λειτουργιών των ανθρωπίνων ωχρινοποιημένων κοκκωδών κυττάρων. [Thesis]. Democritus University of Thrace (DUTH); Δημοκρίτειο Πανεπιστήμιο Θράκης (ΔΠΘ); 2013. Available from: http://hdl.handle.net/10442/hedi/29362

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

26. Dunning, Kylie Renee. Functional characterisation of the cumulus oocyte matrix during maturation of oocytes.

Degree: 2008, University of Adelaide

URL: http://hdl.handle.net/2440/50728

► Female gametes, or oocytes grow and mature in a niche environment maintained by the somatic cells of the ovarian follicle. At ovulation ovarian follicle cells… (more)

▼ Female gametes, or oocytes grow and mature in a niche environment maintained by the somatic cells of the ovarian follicle. At ovulation ovarian follicle cells respond to the luteinising hormone (LH) surge coordinating the final maturation, meiotic resumption and release of oocytes. Simultaneously, production of a unique “mucified” extracellular matrix surrounding the oocyte through synthesis of Hyaluronan (HA) and HA cross-linking proteins produces an “expanded” and stabilised cumulus oocyte matrix with a specific composition, structure and function. In vitro maturation (IVM) of oocytes is a procedure by which cumulus oocyte complexes (COCs) are stimulated to produce cumulus matrix and undergo oocyte maturation ex vivo. In vitro maturation is a useful procedure for studying oocyte competence as well as offering health benefits for patients undergoing assisted reproduction. Oocytes derived from IVM have much lower developmental competence than in vivo matured oocytes, likely as a result of altered environmental conditions and gene expression leading to suboptimal maturation and/or inappropriate metabolic control in oocytes. Cumulus matrix expansion is widely used as an indicator of good oocyte developmental potential, however, the mechanism(s) that endow oocyte quality and how these may be influenced by the cumulus matrix are poorly understood. To better understand the process by which cumulus matrix is linked to the final stages of oocyte maturation, I undertook investigation of mouse COC matrix composition and function after in vivo maturation in comparison to IVM. The gene responsible for Hyaluronan synthesis, Has2, was not impaired under IVM conditions. In contrast, two key extracellular matrix proteins; Versican and Adamts1, which are normally selectively incorporated into periovulatory COCs in vivo, were greater than 10-fold reduced in IVM whether stimulated with Egf and/or FSH. This work is the first to show that commonly used IVM conditions result in altered gene expression in cumulus cells. Furthermore, the absence of Adamts1 and Versican suggest that COC matrix may be functionally insufficient. Although associated with good developmental potential, the function of the COC matrix in oocyte maturation is unknown. I assessed the properties of COC matrix that control metabolite supply to oocytes by examining transport of fluorescently labelled glucose and cholesterol across mouse COCs. Profound differences in the control of metabolite supply to oocytes in IVM were observed. In vivo matured complexes were capable of excluding glucose from the entire COC and cholesterol was excluded from oocytes. Conversely IVM COCs were more permissive to rapid equilibration of glucose and cholesterol concentrations across the complex and in oocytes. In fact both metabolites accumulated rapidly in IVM oocytes resulting in inverse gradient patterns of glucose and cholesterol abundance with highest concentrations accumulating in the oocyte after IVM vs highest concentrations surrounding the COC after in vivo maturation… Advisors/Committee Members: Russell, Darryl Lyndon (advisor), Robker, Rebecca Louise (advisor), School of Paediatrics and Reproductive Health : Obstetrics and Gynaecology (school).

Subjects/Keywords: oocyte maturation; cumulus cells; mural granulosa cells; ovary; assisted reproductive technology; extracellular matrix

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dunning, K. R. (2008). Functional characterisation of the cumulus oocyte matrix during maturation of oocytes. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/50728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dunning, Kylie Renee. “Functional characterisation of the cumulus oocyte matrix during maturation of oocytes.” 2008. Thesis, University of Adelaide. Accessed March 08, 2021. http://hdl.handle.net/2440/50728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dunning, Kylie Renee. “Functional characterisation of the cumulus oocyte matrix during maturation of oocytes.” 2008. Web. 08 Mar 2021.

Vancouver:

Dunning KR. Functional characterisation of the cumulus oocyte matrix during maturation of oocytes. [Internet] [Thesis]. University of Adelaide; 2008. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2440/50728.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dunning KR. Functional characterisation of the cumulus oocyte matrix during maturation of oocytes. [Thesis]. University of Adelaide; 2008. Available from: http://hdl.handle.net/2440/50728

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Montréal

27. Lapointe, Evelyne. Régulation de la fonction ovarienne par la voie de signalisation des WNTs.

Degree: 2012, Université de Montréal

URL: http://hdl.handle.net/1866/8924

Subjects/Keywords: Cellules de la granulosa; Fertilité; Fzd; Ovaire; Wnt; Fertility; Granulosa cells; Ovary; Biology - Molecular / Biologie - Biologie moléculaire (UMI : 0307)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lapointe, E. (2012). Régulation de la fonction ovarienne par la voie de signalisation des WNTs. (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/8924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lapointe, Evelyne. “Régulation de la fonction ovarienne par la voie de signalisation des WNTs.” 2012. Thesis, Université de Montréal. Accessed March 08, 2021. http://hdl.handle.net/1866/8924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lapointe, Evelyne. “Régulation de la fonction ovarienne par la voie de signalisation des WNTs.” 2012. Web. 08 Mar 2021.

Vancouver:

Lapointe E. Régulation de la fonction ovarienne par la voie de signalisation des WNTs. [Internet] [Thesis]. Université de Montréal; 2012. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1866/8924.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lapointe E. Régulation de la fonction ovarienne par la voie de signalisation des WNTs. [Thesis]. Université de Montréal; 2012. Available from: http://hdl.handle.net/1866/8924

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oulu

28. Jääskeläinen, M. (Minna). Apoptosis-regulating factors in developing and adult ovaries.

Degree: 2010, University of Oulu

URL: http://urn.fi/urn:isbn:9789514263477

► Abstract Apoptosis plays a crucial part in human ovarian function from fetal development to the end of reproductive potential. Failures in the regulation of ovarian… (more)

▼ Abstract Apoptosis plays a crucial part in human ovarian function from fetal development to the end of reproductive potential. Failures in the regulation of ovarian apoptosis are associated with many pathological conditions such as premature ovarian insufficiency, infertility and cancer. The purpose of the present study was to analyze the factors regulating cell survival in human fetal and adult ovaries. The fetus is exposed to maternal- and placental-derived estrogens and insufficient estrogen action has destructive effects on rodent ovarian development. We detected estrogen receptors and estrogen-converting enzymes in human fetal ovaries after primordial follicle formation, indicating that estrogens participate in human fetal ovarian development, especially after folliculogenesis. The WNT4 gene is crucial for female sexual differentiation, follicle formation and oocyte survival. We detected WNT4 in follicular cells of fetal and adult human ovaries. In addition, Wnt4- knockout mice demonstrated a dramatic loss of oocytes before birth. However, no changes were detected in protein expression patterns of common apoptosis-related proteins. The results support the possible role of WNT4 in human ovarian function and strengthen previous knowledge on the antiapoptotic role of Wnt4. Apoptosis signaling is mediated by extracellular- and mitochondria-associated- pathways, ending in caspase cascade activation and fragmentation of cellular structures. In the present study we analyzed the expression of several apoptosis-related factors and detected TRAIL, TNF, Bcl-XL, Bok and caspase-3 in human ovaries. In addition, TRAIL was found to be a potent and rapid inducer of human granulosa tumor cell (KGN) apoptosis. Lentiviral downregulation of Bok or Bcl-XL protein expression in KGN cells also resulted in significant changes in cell vulnerability to apoptosis. The results show for the first time the spatiotemporal expression patterns of TRAIL, TNF, Bcl-XL, Bok and caspase-3 in human ovaries and suggest an important functional role of TRAIL, Bok and Bcl-XL in regulation of human ovarian apoptosis. The present study offers novel information on the expression and function of cell survival factors in human ovaries. These new findings open possibilities for future clinical research in attempts to understand and treat ovarian diseases caused by imbalanced regulatory pathways of apoptosis. Advisors/Committee Members: Vaskivuo, T. (Tommi), Tapanainen, J. (Juha).

Subjects/Keywords: TNF-related apoptosis-inducing ligand; Wnt proteins; apoptosis; caspase 3; estrogens; granulosa cells; oocytes; ovary; proto-oncogen proteins c-Bcl-2; tumor necrosis factor- alpha

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jääskeläinen, M. (. (2010). Apoptosis-regulating factors in developing and adult ovaries. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9789514263477

Chicago Manual of Style (16^th Edition):

Jääskeläinen, M (Minna). “Apoptosis-regulating factors in developing and adult ovaries.” 2010. Doctoral Dissertation, University of Oulu. Accessed March 08, 2021. http://urn.fi/urn:isbn:9789514263477.

MLA Handbook (7^th Edition):

Jääskeläinen, M (Minna). “Apoptosis-regulating factors in developing and adult ovaries.” 2010. Web. 08 Mar 2021.

Vancouver:

Jääskeläinen M(. Apoptosis-regulating factors in developing and adult ovaries. [Internet] [Doctoral dissertation]. University of Oulu; 2010. [cited 2021 Mar 08]. Available from: http://urn.fi/urn:isbn:9789514263477.

Council of Science Editors:

Jääskeläinen M(. Apoptosis-regulating factors in developing and adult ovaries. [Doctoral Dissertation]. University of Oulu; 2010. Available from: http://urn.fi/urn:isbn:9789514263477

University of Michigan

29. Bagavandoss, P. Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells.

Degree: PhD, Biology, 1986, University of Michigan

URL: http://hdl.handle.net/2027.42/127841

► This thesis investigates the mechanisms by which retinol (ROH) and retinoic acid (RA) enhance the induction of luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptors and… (more)

Subjects/Keywords: Acid; Action; Cells; Granulosa; Induction; Lh; Luteal; Mechanism; Progesterone; Receptor; Retinoic; Retinol; Synthesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bagavandoss, P. (1986). Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/127841

Chicago Manual of Style (16^th Edition):

Bagavandoss, P. “Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells.” 1986. Doctoral Dissertation, University of Michigan. Accessed March 08, 2021. http://hdl.handle.net/2027.42/127841.

MLA Handbook (7^th Edition):

Bagavandoss, P. “Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells.” 1986. Web. 08 Mar 2021.

Vancouver:

Bagavandoss P. Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells. [Internet] [Doctoral dissertation]. University of Michigan; 1986. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2027.42/127841.

Council of Science Editors:

Bagavandoss P. Mechanism Of Action Of Retinoic Acid And Retinol On Lh Receptor Induction And Progesterone Synthesis In Granulosa And Luteal Cells. [Doctoral Dissertation]. University of Michigan; 1986. Available from: http://hdl.handle.net/2027.42/127841

Washington State University

30. [No author]. YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells .

Degree: 2016, Washington State University

URL: http://hdl.handle.net/2376/12095

► Within the ovarian follicle, immature oocytes are surrounded and supported by granulosa cells (GCs). Stimulation of GCs by follicle stimulating hormone (FSH) promotes their proliferation… (more)

▼ Within the ovarian follicle, immature oocytes are surrounded and supported by granulosa cells (GCs). Stimulation of GCs by follicle stimulating hormone (FSH) promotes their proliferation and differentiation, events that are necessary for fertility. FSH activates multiple signaling pathways to regulate genes necessary for follicular maturation. This research focuses on the regulation by FSH of extracellular signal-regulated kinase (ERK) and identifies Y-box binding protein-1 (YB-1) as a downstream component of ERK signaling. FSH-dependent ERK(Thr202/Tyr204) phosphorylation is protein kinase A (PKA) dependent, yet requires the constitutively active upstream ERK signaling pathway proteins. Treatment with EGFR inhibitor AG1478, a dominant-negative RAS, and the MEK inhibitor PD98059 all blocked FSH-dependent ERK(Thr202/Tyr204) phosphorylation. We hypothesized that FSH via PKA regulates ERK phosphorylation by inhibiting the activity of a protein phosphatase that dephosphorylates ERK in the absence of FSH. Results show that treatment with MAP kinase phosphatase 3 (dual specificity phosphatase 6) (MKP3(DUSP6)) inhibitors increase ERK(Thr202/Tyr204) phosphorylation in the absence of FSH to levels comparable to ERK phosphorylated in the presence of FSH. Further, ERK coimmunoprecipitated with MKP3(DUSP6), and treatment with MKP3(DUSP6) inhibitors blocked dephosphorylation of recombinant phospho-ERK2-GST. Together these results indicate that MKP3(DUSP6) is the phosphatase that dephosphorylates ERK in the absence of FSH. We further identified YB-1 as a downstream target of ERK. YB-1 is a nucleic acid binding protein that regulates transcription and translation. Our results show that FSH promotes an increase in the phosphorylation of YB-1 on Ser102 within 15 min that is maintained until ~8 h post treatment. FSH-stimulated phosphorylation of YB-1(Ser102) is prevented by pretreatment of GCs with the PKA-selective inhibitor PKI, the MEK inhibitor PD98059, and the ribosomal S6 kinase-2 (RSK-2) inhibitor BI-D1870. Transduction of GCs with the dephospho-adenoviral-YB-1(S102A) mutant prevented the FSH-dependent induction of Egfr, Cyp19a1, and Inha mRNAs. Collectively, these results demonstrate novel regulation of ERK(Thr202/Tyr204) phosphorylation by FSH and identify ERK-dependent gene targets that are required for follicular maturation. These results reveal that the ERK signaling pathways contributes to GC maturation by promoting the phosphorylation of the transcriptional activator YB-1 that is required for expression of at least three crucial FSH target genes. Advisors/Committee Members: Hunzicker-Dunn, Mary E (advisor).

Subjects/Keywords: Molecular biology; Cellular biology; Follicle Simulating Hormone (FSH); granulosa cells; ovary; Protein kinase A (PKA); signaling; Y-box binding protein -1 (YB1)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

author], [. (2016). YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells . (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/12095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells .” 2016. Thesis, Washington State University. Accessed March 08, 2021. http://hdl.handle.net/2376/12095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells .” 2016. Web. 08 Mar 2021.

Vancouver:

author] [. YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells . [Internet] [Thesis]. Washington State University; 2016. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2376/12095.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. YB1: A New Player in FSH Regulation of Gene Expression in Granulosa Cells . [Thesis]. Washington State University; 2016. Available from: http://hdl.handle.net/2376/12095

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations