subject:(glutathione)

University of Waterloo

1. Wang, Yiran. Extraction and Concentration of Glutathione from Yeast.

Degree: 2018, University of Waterloo

► Glutathione is a tripeptide present in most mammalian tissue. It has a great variety of significant physiological functions, such as regulating cell growth and division;… (more)

Subjects/Keywords: glutathione

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APA (6^th Edition):

Wang, Y. (2018). Extraction and Concentration of Glutathione from Yeast. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/13831

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Yiran. “Extraction and Concentration of Glutathione from Yeast.” 2018. Thesis, University of Waterloo. Accessed August 23, 2019. http://hdl.handle.net/10012/13831.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Yiran. “Extraction and Concentration of Glutathione from Yeast.” 2018. Web. 23 Aug 2019.

Vancouver:

Wang Y. Extraction and Concentration of Glutathione from Yeast. [Internet] [Thesis]. University of Waterloo; 2018. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/10012/13831.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang Y. Extraction and Concentration of Glutathione from Yeast. [Thesis]. University of Waterloo; 2018. Available from: http://hdl.handle.net/10012/13831

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Wollongong

2. Smith, Cassandra Lee. Modes of copper toxicity in marine microalgae: an intracellular investigation.

Degree: PhD, 2014, University of Wollongong

URL: 060199 Biochemistry and Cell Biology not elsewhere classified ; https://ro.uow.edu.au/theses/4271

► Toxicity of metals to aquatic organisms is dependent on both external factors, such as exposure concentration and water quality parameters, and intracellular processes including… (more)

Subjects/Keywords: Reduced glutathione Glutathione Metallothionein Phytochelatin

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APA (6^th Edition):

Smith, C. L. (2014). Modes of copper toxicity in marine microalgae: an intracellular investigation. (Doctoral Dissertation). University of Wollongong. Retrieved from 060199 Biochemistry and Cell Biology not elsewhere classified ; https://ro.uow.edu.au/theses/4271

Chicago Manual of Style (16^th Edition):

Smith, Cassandra Lee. “Modes of copper toxicity in marine microalgae: an intracellular investigation.” 2014. Doctoral Dissertation, University of Wollongong. Accessed August 23, 2019. 060199 Biochemistry and Cell Biology not elsewhere classified ; https://ro.uow.edu.au/theses/4271.

MLA Handbook (7^th Edition):

Smith, Cassandra Lee. “Modes of copper toxicity in marine microalgae: an intracellular investigation.” 2014. Web. 23 Aug 2019.

Vancouver:

Smith CL. Modes of copper toxicity in marine microalgae: an intracellular investigation. [Internet] [Doctoral dissertation]. University of Wollongong; 2014. [cited 2019 Aug 23]. Available from: 060199 Biochemistry and Cell Biology not elsewhere classified ; https://ro.uow.edu.au/theses/4271.

Council of Science Editors:

Smith CL. Modes of copper toxicity in marine microalgae: an intracellular investigation. [Doctoral Dissertation]. University of Wollongong; 2014. Available from: 060199 Biochemistry and Cell Biology not elsewhere classified ; https://ro.uow.edu.au/theses/4271

University of Hawaii – Manoa

3. Pang, Xiaosha. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.

Degree: 2016, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100634

►

Ph.D. University of Hawaii at Manoa 2013.

Currently 34 million people live with human immunodeficiency virus (HIV) infection. Although the lifespan of HIV patients has… (more)

▼

Ph.D. University of Hawaii at Manoa 2013.

Currently 34 million people live with human immunodeficiency virus (HIV) infection. Although the lifespan of HIV patients has been prolonged by highly active antiretroviral treatment, the prevalence of HIV-associated neurocognitive disorders (HANDs) is increasing. Oxidative stress and inflammation in the central nervous system (CNS) are major causes of HANDs, and concurrent drug abuse may accelerate the progression of symptoms. This dissertation aims to study HIV-induced changes in antioxidants, oxidative stress, and inflammation in the CNS, assess the effects of concurrent methamphetamine (Meth) exposure, and evaluate the protective effect of dietary supplementation of bamboo Phyllostachys edulis extract (BEX). This study centered on the most abundant antioxidant in the brain-glutathione (GSH). The changes of GSH metabolism and GSH-dependent antioxidant enzymes were measured in cerebrospinal fluid (CSF) of human cohorts with HIV infection and/or uses of Meth, and in the brain tissues of HIV-1 model transgenic rats (HIV-1Tg expressing 7 HIV viral proteins) with or without Meth exposure. HIV infection resulted in elevated gamma-glutamyl transpeptidase (GGT) activity, GSH depletion and a several-fold increase in lipid peroxidation in human CSF. Compared to HIV infection, use of Meth resulted in less severe oxidative stress, which may be partially explained by the upregulation of glutathione peroxidase (GPx) and GSH in the CSF. Meth did not interact with HIV in modulating the redox changes in the CSF. In HIV-1Tg rats, redox and inflammatory changes were observed in the brain in a region-specific manner, with little synergy from Meth exposure. The thalamus was highlighted by its high GSH content and systematic upregulation of GSH biosynthesis and GSH-dependent antioxidant enzymes in the HIV-1Tg rats. On the other hand, neuroinflammation markers, such as glial fibrillary acidic protein (GFAP) and p65, were increased in the hippocampus of the transgenic rats, and such changes were effectively normalized by dietary supplementation of BEX. In summary, this dissertation documents the significance of GSH and GSH-dependent antioxidant enzymes in regulating the redox status of CNS, and the potential anti-neuroinflammatory effects of BEX in the context of HIV infection.

Subjects/Keywords: glutathione (GSH)

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APA (6^th Edition):

Pang, X. (2016). HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pang, Xiaosha. “HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.” 2016. Thesis, University of Hawaii – Manoa. Accessed August 23, 2019. http://hdl.handle.net/10125/100634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pang, Xiaosha. “HIV-induced redox changes and inflammation in the central nervous system and modulatory factors.” 2016. Web. 23 Aug 2019.

Vancouver:

Pang X. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. [Internet] [Thesis]. University of Hawaii – Manoa; 2016. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/10125/100634.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pang X. HIV-induced redox changes and inflammation in the central nervous system and modulatory factors. [Thesis]. University of Hawaii – Manoa; 2016. Available from: http://hdl.handle.net/10125/100634

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Zhou, Yang. Genetic control of the glutathione redox system throughout the life span.

Degree: MS, Foods and Nutrition, 2016, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/zhou_yang_201608_ms

► Glutathione (GSH), a ubiquitous tripeptide molecule, regulates numerous cellular processes. In biological systems, GSH exists either in its reduced or oxidized (GSSG) forms, and the… (more)

Subjects/Keywords: Glutathione

…Glutathione Phenotypes . �� ... �� ….31 Table 2: HAM Peaks and Protein… …coding Genes Associated with Kidney Glutathione Phenotypes… …43 viii CHAPTER 1 INTRODUCTION Glutathione (GSH, orγ-L-glutamylcysteinylglycine… …and subsequent accumulation of the glutathione dimer GSSG. As a result, GSH/GSSG declines… …general directions for future studies. 2 CHAPTER 2 LITERATURE REVIEW Glutathione Homeostasis…

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APA (6^th Edition):

Zhou, Y. (2016). Genetic control of the glutathione redox system throughout the life span. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/zhou_yang_201608_ms

Chicago Manual of Style (16^th Edition):

Zhou, Yang. “Genetic control of the glutathione redox system throughout the life span.” 2016. Masters Thesis, University of Georgia. Accessed August 23, 2019. http://purl.galileo.usg.edu/uga_etd/zhou_yang_201608_ms.

MLA Handbook (7^th Edition):

Zhou, Yang. “Genetic control of the glutathione redox system throughout the life span.” 2016. Web. 23 Aug 2019.

Vancouver:

Zhou Y. Genetic control of the glutathione redox system throughout the life span. [Internet] [Masters thesis]. University of Georgia; 2016. [cited 2019 Aug 23]. Available from: http://purl.galileo.usg.edu/uga_etd/zhou_yang_201608_ms.

Council of Science Editors:

Zhou Y. Genetic control of the glutathione redox system throughout the life span. [Masters Thesis]. University of Georgia; 2016. Available from: http://purl.galileo.usg.edu/uga_etd/zhou_yang_201608_ms

Oregon State University

5. Siller-Cepeda, Jorge H. The role of glutathione in the development and release of rest on 'Redhaven' peaches.

Degree: PhD, Horticulture, 1991, Oregon State University

URL: http://hdl.handle.net/1957/25780

► A rapid and sensitive HPLC method for quantifying both the reduced and oxidized forms of glutathione simultaneously in woody plant tissues was developed. This method… (more)

▼ A rapid and sensitive HPLC method for quantifying both the reduced and oxidized forms of glutathione simultaneously in woody plant tissues was developed. This method was applicable to a variety of woody plant species and different tissues. Total glutathione content in the bark tissue of seven of the woody plant species tested were lower during active plant growth, and higher in the dormant stage. All tested tissues of peach plants contained glutathione. The highest and lowest content was found in the leaves and roots, respectively. 'Redhaven' peach trees acquired the onset of rest on 1 Oct, before any chilling had been experienced. Maximum rest was reached on 1 Nov after the plants were exposed to 320 CU, and 50% of the buds were broken at 860 CU on 1 Dec. Total budbreak and phytotoxicity induced by hydrogen cyanamide applied to 'Redhaven' peach trees was dependent on concentration and time of application. Phytotoxicity was evident at all application dates, but greatest at the highest concentrations. Plants were more resistant to hydrogen cyanamide at maximum rest. Hydrogen cyanamide induced budbreak was best during the post-rest period. Budbreak and phytotoxicity induced by hydrogen cyanamide applied during the quiescent stage were dependent on posttreatment temperature and concentration. All cyanamide treatments inhibited budbreak at all tested temperatures. The inhibition was greatest at the the lowest temperature and at the highest concentrations. Phytotoxicity was greater at 15C and 35C than at 25C, and increased with increased concentrations. The content of GSH and GSSG in the buds changed with the development of rest. During the early phases of rest, when rest intensity increased, the content of GSH and GSSG decreased (15 Oct) and then increased at maximum rest (1 Nov). Content of GSH continued to increase and peaked on 1 Dec at 860 CU. On 15 Dec when the buds were qxiiescent GSH content decreased. In contrast, contents of GSSG did not drop during cjuiescence and were highest on 15 Dec. GSH content induced by chilling was closely associated with the end of rest. Hydrogen cyanamide treatments caused a rapid decrease of GSH content within 12 hr followed by a large increase 24 hr after application. The changes of GSH induced were inversely proportional to cyanamide concentration. Non-treated plants showed at relatively constant GSH and GSSG content during this period of time. Hydrogen cyanamide treatments increased GSH contents at all application dates. The extent of GSH changes was dependent on the physiological status of the bud and cyanamide concentration. At maximum rest the plants were more resistant to cyanamide and this coincided with the highest induced GSH content as compared to all application dates. Advisors/Committee Members: Fuchigami, Leslie H. (advisor).

Subjects/Keywords: Glutathione

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APA (6^th Edition):

Siller-Cepeda, J. H. (1991). The role of glutathione in the development and release of rest on 'Redhaven' peaches. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/25780

Chicago Manual of Style (16^th Edition):

Siller-Cepeda, Jorge H. “The role of glutathione in the development and release of rest on 'Redhaven' peaches.” 1991. Doctoral Dissertation, Oregon State University. Accessed August 23, 2019. http://hdl.handle.net/1957/25780.

MLA Handbook (7^th Edition):

Siller-Cepeda, Jorge H. “The role of glutathione in the development and release of rest on 'Redhaven' peaches.” 1991. Web. 23 Aug 2019.

Vancouver:

Siller-Cepeda JH. The role of glutathione in the development and release of rest on 'Redhaven' peaches. [Internet] [Doctoral dissertation]. Oregon State University; 1991. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1957/25780.

Council of Science Editors:

Siller-Cepeda JH. The role of glutathione in the development and release of rest on 'Redhaven' peaches. [Doctoral Dissertation]. Oregon State University; 1991. Available from: http://hdl.handle.net/1957/25780

6. Thomas, Nicholas, O. Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity.

Degree: PhD, 2017, Oregon State University

URL: http://hdl.handle.net/1957/61681

► Over the past century, life expectancy in the United States has dramatically increased leading to an increasingly aging population with people reaching, and spending more… (more)

▼ Over the past century, life expectancy in the United States has dramatically increased leading to an increasingly aging population with people reaching, and spending more years in ‘old age’. While this unprecedented shift in population demographics represents great strides for humanity, it is not without cost. One consequence of longer life is the increased accrual of age-associated diseases and chronic pathophysiological conditions. This is evident in the fact that over 80% of Americans over the age of 65 have at least one chronic medical condition [275]. Thus, lifespan has outpaced ‘healthspan’, or the time of one’s life spent free from disease and disuse syndromes. The work in this dissertation is defined by the investigation of “health assurance” biochemical pathways, the failure of which lead to heightened risk for age-related diseases. In particular, I have focused on why resiliency to oxidative stresses decline significantly with age. This focus has led to a research project that ultimately pinpoints a loss in glutathione-dependent defenses as an underlying aging factor, which could enhance risk for a variety of age-related diseases. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a major transcriptional regulator of numerous anti-oxidant, anti-inflammatory, and metabolic genes. We observed that, paradoxically, Nrf2 protein levels decline in the livers of aged rats despite the inflammatory environment evident in that organ. To examine the cause(s) of this loss, we investigated the age-related changes in Nrf2 protein homeostasis and activation in cultured hepatocytes from young (4–6 months) and old (24–28 months) Fischer 344 rats. While no significant age-dependent change in Nrf2 mRNA levels was observed, Nrf2 protein content was attenuated by ~40% with age (p<0.05 , N=4). Treatment with anethole trithione (A3T), along with bortezomib to inhibit degradation of existing protein, caused Nrf2 to accumulate significantly in cells from young animals (p < 0.05), but not old, indicating a lack of new Nrf2 synthesis. We hypothesized that the loss of Nrf2 protein synthesis with age may partly stem from an age-related increase in microRNA inhibition of Nrf2 translation. miRNA-146a, increases by > 2-fold with age and is predicted to bind Nrf2 mRNA. Transfection of hepatocytes from young rats with a miRNA-146a mimic caused a 55% attenuation of Nrf2 translation that paralleled the age-related loss of Nrf2. Overall, these results provide novel insights for the age-related decline in Nrf2 and identify new targets to maintain Nrf2-dependent detoxification with age. Having established that this major transcriptional regulator is attenuated with age, we next examined what effects this would have on toxicological resilience. Isolated hepatocytes from young and old rats were exposed to increasing concentrations of menadione, a vitamin K derivative and redox cycling agent, an LC50 for each age group was established, and results showed a nearly 2-fold increase in susceptibility to menadione (LC50 for young: 405 μM;… Advisors/Committee Members: Hagen, Tory, M. (advisor), Perez, Viviana, I (committee member).

Subjects/Keywords: Glutathione

…2 1.2 Glutathione… …12 1.2.6 Glutathione and detoxification enzymes… …45 Chapter 3. Glutathione Maintenance Mitigates Age Related Susceptibility to Redox Cycling… …61 3.3.9 Glutathione Analysis… …74 Chapter 4. The Age-Related Loss of Mitochondrial Glutathione Exacerbates Menadione…

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APA (6^th Edition):

Thomas, Nicholas, O. (2017). Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/61681

Chicago Manual of Style (16^th Edition):

Thomas, Nicholas, O. “Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity.” 2017. Doctoral Dissertation, Oregon State University. Accessed August 23, 2019. http://hdl.handle.net/1957/61681.

MLA Handbook (7^th Edition):

Thomas, Nicholas, O. “Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity.” 2017. Web. 23 Aug 2019.

Vancouver:

Thomas, Nicholas O. Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity. [Internet] [Doctoral dissertation]. Oregon State University; 2017. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1957/61681.

Council of Science Editors:

Thomas, Nicholas O. Age-related decrease in resilience against acute redox cycling agents: Critical role of declining GSH-dependent detoxification capacity. [Doctoral Dissertation]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61681

7. Baddariyah Sohsansa. Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats .

Degree: คณะวิทยาศาสตร์ ภาควิชาเภสัชวิทยา, 2014, Prince of Songkla University

URL: http://kb.psu.ac.th/psukb/handle/2010/9867

Subjects/Keywords: Glutathione

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APA (6^th Edition):

Sohsansa, B. (2014). Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats . (Thesis). Prince of Songkla University. Retrieved from http://kb.psu.ac.th/psukb/handle/2010/9867

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sohsansa, Baddariyah. “Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats .” 2014. Thesis, Prince of Songkla University. Accessed August 23, 2019. http://kb.psu.ac.th/psukb/handle/2010/9867.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sohsansa, Baddariyah. “Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats .” 2014. Web. 23 Aug 2019.

Vancouver:

Sohsansa B. Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats . [Internet] [Thesis]. Prince of Songkla University; 2014. [cited 2019 Aug 23]. Available from: http://kb.psu.ac.th/psukb/handle/2010/9867.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sohsansa B. Effect of phenethyl isothiocyante (PEITC) and betong watercress (Nasturtium officinale) on glutathione S-transferase activity and glutathione levels in rats . [Thesis]. Prince of Songkla University; 2014. Available from: http://kb.psu.ac.th/psukb/handle/2010/9867

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Texas

8. Ingle, Brandall L. The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis.

Degree: 2015, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc801927/

► Human glutathione synthetase (hGS) is a homodimeric enzymes that catalyzes the second step in the biological synthesis of glutathione, a critical cellular antioxidant. The enzyme… (more)

▼ Human glutathione synthetase (hGS) is a homodimeric enzymes that catalyzes the second step in the biological synthesis of glutathione, a critical cellular antioxidant. The enzyme exhibits negative cooperativity towards the γ-glutamylcysteine (γ-GC) substrate. In this type of allosteric regulation, the binding of γ-GC at one active site significantly reduces substrate affinity at a second active site over 40 Å away. The presented work explores protein-protein interactions, substrate binding, and allosteric communication through investigation of three regions of hGS: the dimer interface, the S-loop, and the E-loop. Strong electrostatic interactions across the dimer interface of hGS maintain the appropriate tertiary and quaternary enzymatic structure needed for activity. The S-loop and E-loop of hGS form walls of the active site near γ-GC, with some residues serving to bind and position the negatively cooperative substrate. These strong interactions in the active site serve as a trigger for allosteric communication, which then passes through hydrophobic interactions at the interface. A comprehensive computational and experimental approach relates hGS structure with activity and regulation. ATP-grasp enzymes, including hGS, utilize ATP in the nucleophilic attack of a carboxylic acid in a reaction thought to proceed through the formation of an acylphosphate intermediate. Small metal cations are known to chelate the terminal phosphates of actives site ATP, yet the role of these atoms remains unclear. In the presented work, a computational metal substitution study establishes the role these divalent cations in the catalysis of peptide bonds. The simple model is used to determine the impact of metal cations on the thermodynamics and kinetics, an important stepping stone in understanding the importance of metal cations in larger biological systems. Advisors/Committee Members: Cundari, Thomas R., 1964-, Omary, Mohammad A., 1969-, Petros, Robby A., Richmond, Michael G., Anderson, Mary E..

Subjects/Keywords: allostery; glutathione; enzyme; catalysis; Glutathione.; Ligases.; Catalysis.

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APA (6^th Edition):

Ingle, B. L. (2015). The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc801927/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ingle, Brandall L. “The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis.” 2015. Thesis, University of North Texas. Accessed August 23, 2019. https://digital.library.unt.edu/ark:/67531/metadc801927/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ingle, Brandall L. “The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis.” 2015. Web. 23 Aug 2019.

Vancouver:

Ingle BL. The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis. [Internet] [Thesis]. University of North Texas; 2015. [cited 2019 Aug 23]. Available from: https://digital.library.unt.edu/ark:/67531/metadc801927/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ingle BL. The Mechanisms of Human Glutathione Synthetase and Related Non-Enyzmatic Catalysis. [Thesis]. University of North Texas; 2015. Available from: https://digital.library.unt.edu/ark:/67531/metadc801927/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

9. Emmert, Sans Wellington. Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds.

Degree: PhD, Molecular Medicine, 2008, Penn State University

URL: https://etda.libraries.psu.edu/catalog/8177

► ABSTRACT Levels of dietary selenium are inversely associated with cancer risk in humans, and selenium has played a major role in the field of chemoprevention.… (more)

▼ ABSTRACT Levels of dietary selenium are inversely associated with cancer risk in humans, and selenium has played a major role in the field of chemoprevention. In particular, the organoselenium agents 1,4-phenylenebis(methylene)seleno-cyanate (p-XSC) and its glutathione conjugate, p-XSeSG, are effective in preventing cancers at numerous sites, including the lung in rodent models. In the A/J mouse lung, p-XSC is highly effective in inducing glutathione (GSH), but mechanisms are not well understood. Glutathione is the most abundant antioxidant in animals, and is an important mechanistic factor in chemoprevention. Glutamate cysteine ligase (GCL) is the rate-limiting enzyme responsible for GSH production. Transcriptional regulation of GCL occurs via antioxidant response elements (ARE) in the promoter regions of its genes. Induction of the ARE is in turn regulated by stabilization and nuclear translocation of the transcription factor, Nuclear factor-erythroid 2-Related Factor 2 (Nrf2). Furthermore, the extracellular signal-regulated kinase (ERK) pathway has been shown to play a role in Nrf2 stabilization. Potent chemopreventive phytochemicals, such as sulforaphane (SFN), have been shown to act largely through the Nrf2/ARE pathway. The potential relevance of Nrf2 to GSH induction led to the hypothesis that Nrf2 activation may be involved in organoselenium-mediated induction of GSH, and this was investigated in vivo and in several cell culture models. Here we show that dietary p-XSC induces Nrf2, p-ERK, GCL and GSH in the lung of the Fisher rat, suggesting involvement of Nrf2 and the ERK pathway in GSH induction. In addition, GSH and GCL were induced by p-XSeSG in the Fisher rat. In cell culture studies we observed that p-XSC and p-XSeSG activate an ARE luciferase reporter in an Nrf2-dependent manner. It was also shown that p-XSeSG induced GSH in vitro, and is less toxic than p-XSC. The dependence of GCL induction by p-XSeSG upon Nrf2 was confirmed in wildtype and Nrf2-mutanted Mouse Embryonic Fibroblasts (MEF). These results suggest that p-XSC acts through the Nrf2 pathway in vivo, and that p-XSeSG may be the metabolite responsible for such activation, thus offering p-XSeSG as a less toxic, yet highly efficacious inducer of GSH. Isothiocyanate compounds such as SFN are among the most potent Nrf2 inducers known. We hypothesized that substitution of sulfur with selenium in the isothiocyanate functional group of SFN would result in an isoselenocyanate compound (SFN-isoSe) with enhanced Nrf2 induction capability. Here we show that SFN-isoSe activates an ARE-luciferase reporter in HepG2 cells more potently than SFN. It was also found that SFN-isoSe induces GCL and GSH in MEF cells in an Nrf2-dependent manner. Finally, we provide evidence that SFN-isoSe is more effective in killing HepG2 cancer cells, yet is less toxic to non-cancer MEF cells, than is SFN. These data support our hypothesis, and suggest that SFN-isoSe may be a highly effective chemoprotective agent in vivo. Taken together, these results support the hypothesis that…

Subjects/Keywords: glutathione; organoselenium; Nrf2

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APA (6^th Edition):

Emmert, S. W. (2008). Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/8177

Chicago Manual of Style (16^th Edition):

Emmert, Sans Wellington. “Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds.” 2008. Doctoral Dissertation, Penn State University. Accessed August 23, 2019. https://etda.libraries.psu.edu/catalog/8177.

MLA Handbook (7^th Edition):

Emmert, Sans Wellington. “Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds.” 2008. Web. 23 Aug 2019.

Vancouver:

Emmert SW. Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds. [Internet] [Doctoral dissertation]. Penn State University; 2008. [cited 2019 Aug 23]. Available from: https://etda.libraries.psu.edu/catalog/8177.

Council of Science Editors:

Emmert SW. Role of Nuclear factor-erythroid 2-Related Factor 2 in Glutamate Cysteine Ligase Induction by Organoselenium Compounds. [Doctoral Dissertation]. Penn State University; 2008. Available from: https://etda.libraries.psu.edu/catalog/8177

University of Utah

10. Raftogianis, Rebecca Blanchard. Total parenteral nutrition alters hepatic drug conjugation in rats;.

Degree: PhD, Pharmaceutics & Pharmaceutical Chemistry;, 1995, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1614/rec/1404

► Total parenteral nutrition (TPN) provides complete nutritional support in settings in which patients cannot eat. TPN, typically administered through a central vein as an elemental… (more)

Subjects/Keywords: Prostaglandin; Glutathione

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APA (6^th Edition):

Raftogianis, R. B. (1995). Total parenteral nutrition alters hepatic drug conjugation in rats;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1614/rec/1404

Chicago Manual of Style (16^th Edition):

Raftogianis, Rebecca Blanchard. “Total parenteral nutrition alters hepatic drug conjugation in rats;.” 1995. Doctoral Dissertation, University of Utah. Accessed August 23, 2019. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1614/rec/1404.

MLA Handbook (7^th Edition):

Raftogianis, Rebecca Blanchard. “Total parenteral nutrition alters hepatic drug conjugation in rats;.” 1995. Web. 23 Aug 2019.

Vancouver:

Raftogianis RB. Total parenteral nutrition alters hepatic drug conjugation in rats;. [Internet] [Doctoral dissertation]. University of Utah; 1995. [cited 2019 Aug 23]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1614/rec/1404.

Council of Science Editors:

Raftogianis RB. Total parenteral nutrition alters hepatic drug conjugation in rats;. [Doctoral Dissertation]. University of Utah; 1995. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1614/rec/1404

University of Utah

11. Phaneuf, Holly Louise. Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;.

Degree: PhD, Biochemistry;, 1997, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1638/rec/1318

► Numerous deleterious physiological effects result from cellular depletion of glutathione (GSH). GSH is abundant in living systems and exerts several protective functions. Reduction of the… (more)

▼ Numerous deleterious physiological effects result from cellular depletion of glutathione (GSH). GSH is abundant in living systems and exerts several protective functions. Reduction of the normally high levels of GSH in cells results from a wide array of endogenous and exogenous factors. The liver is frequently vulnerable to GSH depletion by exposure to toxins. Developing novel agents that increase GSH in the liver has been the primary goal of this work. Since GSH cannot be taken up by cells and is quickly catabolized, prodrug forms of its constituent L-cysteine, which supports the biosynthesis of GSH, have proved a successful therapeutic alternative. Prodrug versions of L-cysteine are required to overcome the toxicity of L-cystelne itself. Drug targeting as a means of directing agents to particular cells or organs is well established in the case of hepatocyte targeting. The hepatic asialoglycoprotein receptor (ASGPR) recognizes molecules bearing galactose moieties, which is followed by cellular uptake. Three L-cysteine prodrugs possessing galactose were synthesized using the condensation of L-cysteine with aldoses to form a thiazolidine carboxylic acid (TCA). TCAs dissociate nonenzymatically over time and have previously demonstrated GSH-maintaining ability. The galactose-bearing prodrugs were developed with the aim of increasing liver L-cysteine prodrug through ASGPR targeting. Two related glucose-bearing prodrugs were synthesized as controls. Prodrug toxicity was not seen in mice and apparent in cells only at high concentrations after prolonged exposures. Biodistribution of prodrugs in mice and protection using hepatocytes with and without the ASGPR were used to indicate targeting. Protection studies used acetaminophen (APAP), which is well documented as a GSH-depleting hepatotoxin. All five prodrugs protected against APAP, yet prodrug targeting is ambiguous. Additional protection mechanisms involving Phase II enzyme induction were addressed, because thiol induction has been documented. Prodrugs did not cause induction at the doses given, with the possible exception of one, but this was complicated by APAP induction. Studies determining liver and kidney GSH imply that the most conspicuous protection mechanism is the prevention of GSH depletion by APAP. Although ASGPR targeting is not immediately obvious from these studies, the principal goal, the development of novel hepatoprotective GSH-maintaining agents, was achieved.

Subjects/Keywords: Physiological; Glutathione

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APA (6^th Edition):

Phaneuf, H. L. (1997). Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1638/rec/1318

Chicago Manual of Style (16^th Edition):

Phaneuf, Holly Louise. “Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;.” 1997. Doctoral Dissertation, University of Utah. Accessed August 23, 2019. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1638/rec/1318.

MLA Handbook (7^th Edition):

Phaneuf, Holly Louise. “Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;.” 1997. Web. 23 Aug 2019.

Vancouver:

Phaneuf HL. Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;. [Internet] [Doctoral dissertation]. University of Utah; 1997. [cited 2019 Aug 23]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1638/rec/1318.

Council of Science Editors:

Phaneuf HL. Synthesis and evaluation of novel hepatoprotective L-cysteine prodrugs;. [Doctoral Dissertation]. University of Utah; 1997. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1638/rec/1318

Texas A&M University

12. Karren, Brady. THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS.

Degree: 2010, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-54

► Quarter Horse mares (n=28, 465-612 kg BW, 6-19 yrs of age) were used to investigate the effect of organic selenium (Se) supplementation (Selenosource, Diamond V… (more)

▼ Quarter Horse mares (n=28, 465-612 kg BW, 6-19 yrs of age) were used to investigate the effect of organic selenium (Se) supplementation (Selenosource, Diamond V Mills, Inc. Cedar Rapids, IA (SeM)) and DE manipulation on plasma, muscle, and colostrum Se concentrations, plasma glutathione peroxidase (Gsh-Px) activity, foaling parameters, and physical characteristics in mares and their foals. Mares were arranged in a 2x2 factorial with two levels of nutrition, pasture (100% NRC DE) or pasture plus grain (120% NRC DE) (fed at 0.75% BW (0.63 ppm Se)) and two levels of Se supplementation (0 or 0.3 mg/kg DM) equaling four treatment groups: pasture (P), pasture grain (PG), pasture grain Se (PGS), or pasture Se (PS). Mares were blocked by expected foaling date and randomly assigned to dietary treatment within block. Body condition score (BCS), BW, and rump fat (RF) were observed every 14 d beginning at d 0. Mare and foal plasma and muscle sampling began on d 0 (birth in foals). Plasma continued every 14 d and muscle every 28 d until parturition (d 56 in foals). Upon parturition, foaling parameters consisting of times: water break to birth, birth to placenta expulsion, foal standing, and nursing were recorded. Colostrum quality was determined via refractometer and colostrometer analysis, and placenta weight, foal birth weight, whither and hip height and body length were recorded. Maternal SeM supplementation influenced (P<0.05) mare and foal plasma, muscle and colostrum Se concentrations. Increased maternal DE influenced (P<0.05) mare and foal plasma and foal muscle Se, mare BW, BCS, and RF. However, mare muscle Se was unaffected (P>0.05) by DE. Mare and foal plasma Gsh-Px, foal physical characteristics, and foaling parameters were unaffected by treatment (P>0.05). Greater (P<0.02) colostrum refractometer values (Brix%) for P, PS mares were noted and PGS, P mares had shorter gestational lengths (nutrition x SeM interaction (P<0.05)). These data indicate that maternal DE manipulation and SeM supplementation influences mare and foal Se status, mare BW and colostrum quality (Brix%), but not plasma Gsh-Px activity. Additionally, nutrition and SeM supplementation may affect gestational length. However, despite treatments there was no difference in foaling parameters or foal physical characteristics. Advisors/Committee Members: Coverdale, Josie A. (advisor), Gibbs, Pete (committee member), Scott, Brett (committee member), Walzem, Rosemary (committee member).

Subjects/Keywords: horse; selenium; glutathione peroxidase

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APA (6^th Edition):

Karren, B. (2010). THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-54

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Karren, Brady. “THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS.” 2010. Thesis, Texas A&M University. Accessed August 23, 2019. http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-54.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Karren, Brady. “THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS.” 2010. Web. 23 Aug 2019.

Vancouver:

Karren B. THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS. [Internet] [Thesis]. Texas A&M University; 2010. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-54.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Karren B. THE EFFECT OF ORGANIC SELENIUM SUPPLEMENTATION AND DIETARY ENERGY MANIPULATION ON MARES AND THEIR FOALS: SELENIUM CONCENTRATIONS, GLUTATHIONE PEROXIDASE ACTIVITY, FOALING PARAMETERS AND FOAL PHYSICAL CHARACTERISTICS. [Thesis]. Texas A&M University; 2010. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2008-08-54

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

13. [No author]. Dynamics of the glutathione/glutaredoxin system.

Degree: Genetics, 2014, University of KwaZulu-Natal

URL: http://hdl.handle.net/10413/12219

► The glutathione/glutaredoxin system is made up of glutaredoxins, glutathione (GSH) and glutathione reductase (GLR). Glutaredoxins, which are involved in essential cellular functions such as DNA… (more)

▼ The glutathione/glutaredoxin system is made up of glutaredoxins, glutathione (GSH) and glutathione reductase (GLR). Glutaredoxins, which are involved in essential cellular functions such as DNA synthesis, iron metabolism and iron-sulfur cluster assembly, become oxidised during their catalytic cycle and are reduced by GSH and GLR. Glutaredoxins also play a critical role in regulating the glutathionylation/deglutathionylation cycle. Under oxidative stress conditions, protein thiols may be glutathionylated and glutaredoxin activity is important for restoring the functions of these proteins. While the individual components of this system have been studied extensively, the dynamics of the system as a whole has not been described despite its importance in the glutathionylation/deglutathionylation process. Computational systems biology approaches could be used to describe this type of regulation but the kinetic mechanism used by glutaredoxins for deglutathionylation is unclear as a monothiol and a dithiol mechanism have both been proposed for glutaredoxin activity. The in vitro data supporting these mechanisms have been contradictory with a number of discrepancies observed in the literature, including contrasting activities of mutant glutaredoxin Cxx(C→S) and wild-type glutaredoxins. Further, Lineweaver-Burk plots showed a curved line pattern in some studies, while other studies reported a linear pattern in response to GSH. Finally, analyses of the Lineweaver-Burk plots in two substrate kinetics experiments revealed both parallel line and intersecting initial velocity line patterns for deglutathionylation. Computational and mathematical models were used to resolve these discrepancies and we showed that the mono- and di- thiol mechanisms, are in fact identical. Mathematical models of mutant and wild-type glutaredoxin activities revealed that the GSH concentration and the rate constant for GSH oxidation significantly affected these relative activities which explained the contradictory data for wild-type and mutant glutaredoxins. The sigmoidal response to GSH was due to the kinetic order of this reaction and our results demonstrated that the resulting parallel and intersecting kinetic line patterns observed in some studies depended on the reversibility of the deglutathionylation reaction. Finally, fitting experiments showed that our models were able to accurately describe the in vitro data. Collectively, our results showed how deglutathionylation should be described in computational systems biology models and further revealed how the formation of oxidised glutaredoxin may play a vital role in the regulation of glutaredoxin activity. Advisors/Committee Members: Pillay, Ché Sobashkar (advisor), Rohwer, Johann M (advisor).

Subjects/Keywords: Glutathione.; Glutaredoxin.; Proteins.; Genetics.

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APA (6^th Edition):

author], [. (2014). Dynamics of the glutathione/glutaredoxin system. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/12219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Dynamics of the glutathione/glutaredoxin system. ” 2014. Thesis, University of KwaZulu-Natal. Accessed August 23, 2019. http://hdl.handle.net/10413/12219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Dynamics of the glutathione/glutaredoxin system. ” 2014. Web. 23 Aug 2019.

Vancouver:

author] [. Dynamics of the glutathione/glutaredoxin system. [Internet] [Thesis]. University of KwaZulu-Natal; 2014. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/10413/12219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Dynamics of the glutathione/glutaredoxin system. [Thesis]. University of KwaZulu-Natal; 2014. Available from: http://hdl.handle.net/10413/12219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Youngstown State University

14. Warren, John C., III. Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption.

Degree: MSin Chemistry, Department of Chemistry, 2011, Youngstown State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1310473424

► Glutathione metabolism differs amongst humans and bacteria and has the potential to be a good target for antibacterial agents. Within <i>Escherichia coli</i>, glutathione can form… (more)

Subjects/Keywords: Chemistry; Gene disruption; Glutathione; Spermidine

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APA (6^th Edition):

Warren, John C., I. (2011). Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption. (Masters Thesis). Youngstown State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ysu1310473424

Chicago Manual of Style (16^th Edition):

Warren, John C., III. “Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption.” 2011. Masters Thesis, Youngstown State University. Accessed August 23, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1310473424.

MLA Handbook (7^th Edition):

Warren, John C., III. “Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption.” 2011. Web. 23 Aug 2019.

Vancouver:

Warren, John C. I. Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption. [Internet] [Masters thesis]. Youngstown State University; 2011. [cited 2019 Aug 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1310473424.

Council of Science Editors:

Warren, John C. I. Evaluation of the Role of gsp, ygiC, and yjfC Genes in Glutathione Metabolism in Escherichia coli by Gene Disruption. [Masters Thesis]. Youngstown State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1310473424

Youngstown State University

15. Gadapa, Sirisha. Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli.

Degree: MSin Chemistry, Department of Chemistry, 2011, Youngstown State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311165527

► Glutathione (¿-Glu-Cys-Gly) is a tripeptide-, and a primary thiol found in most organisms. It regulates intracellular thiol levels and maintains redox balance. In <i>Escherichia coli</i>… (more)

Subjects/Keywords: Biochemistry; Chemistry; Glutathione; Glutathionylspermidine

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APA (6^th Edition):

Gadapa, S. (2011). Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli. (Masters Thesis). Youngstown State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311165527

Chicago Manual of Style (16^th Edition):

Gadapa, Sirisha. “Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli.” 2011. Masters Thesis, Youngstown State University. Accessed August 23, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311165527.

MLA Handbook (7^th Edition):

Gadapa, Sirisha. “Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli.” 2011. Web. 23 Aug 2019.

Vancouver:

Gadapa S. Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli. [Internet] [Masters thesis]. Youngstown State University; 2011. [cited 2019 Aug 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311165527.

Council of Science Editors:

Gadapa S. Purification and Characterization of Putative Glutathionylspermidine synthetase, YgiC from Escherichia coli. [Masters Thesis]. Youngstown State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1311165527

University of Hong Kong

16. Wang, Siqi. The role of GSTP1 in non-small cell lung cancer.

Degree: Master of Medical Sciences, 2015, University of Hong Kong

URL: Wang, S. [王思騏]. (2015). The role of GSTP1 in non-small cell lung cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5659717 ; http://hdl.handle.net/10722/221491

►

The unsatisfactory outcomes often encountered in the treatment of non-small cell lung cancers can be attributed to multiple drug resistance, tumour metastases, and tumour recrudescence.… (more)

▼

The unsatisfactory outcomes often encountered in the treatment of non-small cell lung cancers can be attributed to multiple drug resistance, tumour metastases, and tumour recrudescence. Recently, these features are suggested to be modulated by cancer stem cells (CSC) which is a subpopulation of tumour cells with special phenotypic characteristics such as enhanced survival, resistance to toxicity, cell invasion and migration, as well as maintenance of low intracellular levels of reactive oxygen species (ROS). Targeting CSC has been suggested as an essential approach for long-term eradication of tumours. Previous data have demonstrated Ca2+and calmodulin-dependent protein kinase Ⅱα (CaMKⅡα) could regulate CSC, and CSC isolated from NSCLC cell lines using well recognized surface markers contained lower levels of ROS. Accordingly, this study hypothesizes CaMKⅡα could play a role in the control of ROS levels through regulation of REDOX enzymes. To investigate this possibility, the effects of CaMKⅡα on the expression of the master ROS regulator nuclear factor erythroid-2-related factor 2 (NFR2) and its downstream targets were examined using in vitro models. Results from western blot analysis showed a higher level of activated, nuclear NRF2in cells over-expressing CaMKⅡα than that in control cells. Furthermore, amongst five NRF2 downstream targets, glutathione S-transferase P1 (GSTP1) showed the most consistent change in mRNA expression upon CaMKⅡα manipulation by pharmacologic or genetic approaches. In the literature, GSTP1 has not been shown to affect CSC properties. Thus, the in vitro analysis of self-renewal ability was studied in NSCLC cells with transient GSTP1 knockdown, which led to reduced cell sphere and colony formation, respectively. Together, the data showed maintenance of low intracellular ROS levels by regulation of NRF2/GSTP1 expression is a potential mechanism through which CaMKⅡα modulates CSC self-renewal capacity. The anti-oxidant action of CaMKⅡα/NRF2/GSTP1 pathway might be a novel treatment for NSCLC. Further studies using stable genetic manipulation of GSTP1 and in vivo models would provide further support for this hypothesis.

published_or_final_version

Pathology

Master

Master of Medical Sciences

Subjects/Keywords: Glutathione transferase; Lungs - Cancer

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APA (6^th Edition):

Wang, S. (2015). The role of GSTP1 in non-small cell lung cancer. (Masters Thesis). University of Hong Kong. Retrieved from Wang, S. [王思騏]. (2015). The role of GSTP1 in non-small cell lung cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5659717 ; http://hdl.handle.net/10722/221491

Chicago Manual of Style (16^th Edition):

Wang, Siqi. “The role of GSTP1 in non-small cell lung cancer.” 2015. Masters Thesis, University of Hong Kong. Accessed August 23, 2019. Wang, S. [王思騏]. (2015). The role of GSTP1 in non-small cell lung cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5659717 ; http://hdl.handle.net/10722/221491.

MLA Handbook (7^th Edition):

Wang, Siqi. “The role of GSTP1 in non-small cell lung cancer.” 2015. Web. 23 Aug 2019.

Vancouver:

Wang S. The role of GSTP1 in non-small cell lung cancer. [Internet] [Masters thesis]. University of Hong Kong; 2015. [cited 2019 Aug 23]. Available from: Wang, S. [王思騏]. (2015). The role of GSTP1 in non-small cell lung cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5659717 ; http://hdl.handle.net/10722/221491.

Council of Science Editors:

Wang S. The role of GSTP1 in non-small cell lung cancer. [Masters Thesis]. University of Hong Kong; 2015. Available from: Wang, S. [王思騏]. (2015). The role of GSTP1 in non-small cell lung cancer. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5659717 ; http://hdl.handle.net/10722/221491

University of Hong Kong

17. 洪禕璠; Hong, Yifan. Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism.

Degree: PhD, 2013, University of Hong Kong

URL: Hong, Y. [洪禕璠]. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435638 ; http://dx.doi.org/10.5353/th_b5435638 ; http://hdl.handle.net/10722/209469

published_or_final_version

Chemistry

Doctoral

Doctor of Philosophy

Subjects/Keywords: Bismuth; Glutathione

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APA (6^th Edition):

洪禕璠; Hong, Y. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Doctoral Dissertation). University of Hong Kong. Retrieved from Hong, Y. [洪禕璠]. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435638 ; http://dx.doi.org/10.5353/th_b5435638 ; http://hdl.handle.net/10722/209469

Chicago Manual of Style (16^th Edition):

洪禕璠; Hong, Yifan. “Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism.” 2013. Doctoral Dissertation, University of Hong Kong. Accessed August 23, 2019. Hong, Y. [洪禕璠]. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435638 ; http://dx.doi.org/10.5353/th_b5435638 ; http://hdl.handle.net/10722/209469.

MLA Handbook (7^th Edition):

洪禕璠; Hong, Yifan. “Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism.” 2013. Web. 23 Aug 2019.

Vancouver:

洪禕璠; Hong Y. Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. [Internet] [Doctoral dissertation]. University of Hong Kong; 2013. [cited 2019 Aug 23]. Available from: Hong, Y. [洪禕璠]. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435638 ; http://dx.doi.org/10.5353/th_b5435638 ; http://hdl.handle.net/10722/209469.

Council of Science Editors:

洪禕璠; Hong Y. Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. [Doctoral Dissertation]. University of Hong Kong; 2013. Available from: Hong, Y. [洪禕璠]. (2013). Positive feedback between bismuth uptake and glutathione biosynthesis in human cell and bismuth's impacts on metal metabolism. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5435638 ; http://dx.doi.org/10.5353/th_b5435638 ; http://hdl.handle.net/10722/209469

University of British Columbia

18. Guo, Ningning. GSH : a new candidate neuropeptide in the CNS .

Degree: 1991, University of British Columbia

URL: http://hdl.handle.net/2429/29856

► The physiological significance of glutathione (GSH) in the mammalian central nervous system is still uncertain, although some evidence has indicated that GSH may play an… (more)

Subjects/Keywords: Glutathione; Neuropeptides

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APA (6^th Edition):

Guo, N. (1991). GSH : a new candidate neuropeptide in the CNS . (Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/29856

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guo, Ningning. “GSH : a new candidate neuropeptide in the CNS .” 1991. Thesis, University of British Columbia. Accessed August 23, 2019. http://hdl.handle.net/2429/29856.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guo, Ningning. “GSH : a new candidate neuropeptide in the CNS .” 1991. Web. 23 Aug 2019.

Vancouver:

Guo N. GSH : a new candidate neuropeptide in the CNS . [Internet] [Thesis]. University of British Columbia; 1991. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/2429/29856.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guo N. GSH : a new candidate neuropeptide in the CNS . [Thesis]. University of British Columbia; 1991. Available from: http://hdl.handle.net/2429/29856

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

19. Sherman, Morris. Clinical and biochemical studies on the gluthathione S-transferases.

Degree: Image, Division of Chemical Pathology, 1982, University of Cape Town

URL: http://hdl.handle.net/11427/27261

► The glutathione S-transferases (ligandins) are a ubiquitous system of xenobiotic metabolising enzymes. In the rat liver they comprise up to 10% of soluble hepatic protein.… (more)

▼ The glutathione S-transferases (ligandins) are a ubiquitous system of xenobiotic metabolising enzymes. In the rat liver they comprise up to 10% of soluble hepatic protein. Studies in the rat suggested that ligandin was an accurate and sensitive marker of hepatocellular necrosis. and of renal tubular necrosis. The first part of this thesis examines the release of ligandin from liver and kidney in human liver and renal disease in an attempt to determine whether the measurement of ligandin is clinically useful. Ligandin was purified from human liver cytosol using a combination of anion exchange chromatography and gel filtration. The purified protein had similar physicochemical characteristics to ligandin purified by others. The protein was used to raise a monospecific antibody. Ligandin was iodinated by the Chloramine-T method. which yielded a labelled protein of high specific activity. A sensitive and specific radioimmunoassay for human ligandin was developed which had a low intra- and interassay variation. The assay was applied to the study of human liver disease. In acute hepatitis ligandin is released from the liver into serum early in the illness. High serum ligandin levels are seen in the first week of acute hepatitis. The rapid return to normal suggests that ligandin may provide an early indication of recovery. In chronic hepatitis ligandin levels correlated significantly with histological severity of disease. whereas SGOT showed no such correlation. Ligandin may be a better index of severity of disease and for treatment than SGOT. Ligandin was released from the kidney in severe renal ischaemia and in acute tubular necrosis, but was not a reliable predictor or indicator of acute tubular necrosis. Part two examines the distribution of GSH-T activity in organs and in hepatocellular carcinoma. Ligandin was shown to be immunologically similar in all tissues studied. Isoelectric focusing of cytosol separated the three groups of GSH-T activity. Considerable variety in the distribution and activity of GSH-T's was shown in different organs from a single donor, and in the same organs from different donors. Anionic transferase activity was shown to contribute a significant proportion of activity in organs other than the liver. and to be the major source of activity in ovary and lung. In hepatocellular carcinoma cationic GSH-T activity was present in amounts varying from near normal to absent. The anionic and neutral GSH-T's were present in amounts similar to that seen in normal liver. Immunohistochemical studies using a peroxidase-antiperoxidase method showed a rough correlation between tumour differentiation and the amount of ligandin in the tumour. Advisors/Committee Members: Kirsch, Ralph E (advisor).

Subjects/Keywords: Glutathione transferases

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APA (6^th Edition):

Sherman, M. (1982). Clinical and biochemical studies on the gluthathione S-transferases. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/27261

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sherman, Morris. “Clinical and biochemical studies on the gluthathione S-transferases.” 1982. Thesis, University of Cape Town. Accessed August 23, 2019. http://hdl.handle.net/11427/27261.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sherman, Morris. “Clinical and biochemical studies on the gluthathione S-transferases.” 1982. Web. 23 Aug 2019.

Vancouver:

Sherman M. Clinical and biochemical studies on the gluthathione S-transferases. [Internet] [Thesis]. University of Cape Town; 1982. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/11427/27261.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sherman M. Clinical and biochemical studies on the gluthathione S-transferases. [Thesis]. University of Cape Town; 1982. Available from: http://hdl.handle.net/11427/27261

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

20. Thumser, Alfred Ernst Adolf. The glutathione S-transferases : inhibition, activation, binding and kinetics.

Degree: Image, Division of Medical Biochemistry, 1990, University of Cape Town

URL: http://hdl.handle.net/11427/28958

Subjects/Keywords: Glutathione transferases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thumser, A. E. A. (1990). The glutathione S-transferases : inhibition, activation, binding and kinetics. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/28958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thumser, Alfred Ernst Adolf. “The glutathione S-transferases : inhibition, activation, binding and kinetics.” 1990. Thesis, University of Cape Town. Accessed August 23, 2019. http://hdl.handle.net/11427/28958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thumser, Alfred Ernst Adolf. “The glutathione S-transferases : inhibition, activation, binding and kinetics.” 1990. Web. 23 Aug 2019.

Vancouver:

Thumser AEA. The glutathione S-transferases : inhibition, activation, binding and kinetics. [Internet] [Thesis]. University of Cape Town; 1990. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/11427/28958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thumser AEA. The glutathione S-transferases : inhibition, activation, binding and kinetics. [Thesis]. University of Cape Town; 1990. Available from: http://hdl.handle.net/11427/28958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Victoria University of Wellington

21. Pal, Ramavati. Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina.

Degree: 2010, Victoria University of Wellington

URL: http://hdl.handle.net/10063/1541

► The glutathione transferases are a family of multifunctional enzymes involved in detoxification of xenobiotic and endogenous electrophilic compounds. Interest in insect GSTs has primarily focused… (more)

▼ The glutathione transferases are a family of multifunctional enzymes involved in detoxification of xenobiotic and endogenous electrophilic compounds. Interest in insect GSTs has primarily focused on their role in insecticide resistance. The sheep blowfly, Lucilia cuprina is a major economic problem for the sheep meat and wool industries in Australasia and hence this thesis has attempted the study of the Lucilia cuprina GST family, using proteomics, with a view to eventually determining their role in insecticide resistance. Combinations of different affinity matrices (glutathione-Sepharose matrix (GSH) followed by dinitrophenyl-glutathione-Sepharose matrix (DNP-GSH)) and two-dimensional electrophoresis has successfully isolated members from major four insect GST classes: Sigma, Delta, Epsilon and Omega. Drosophila melanogaster has been used as a model insect throughout as a basis for comparison. To characterise Lucilia GSTs, the whole metazoan fragmentation database was used for sequence alignment with Lucilia peptides. This approach is broad and speculative but predicts a possible classification of the GSTs based on % similarity and % identity. This method of characterisation yielded match scores that provided a basis for classification, which must at present be regarded as tentative and in need of confirmation. In D. melanogaster and L. cuprina, GSH affinity-purified extracts showed the presence of only Sigma and Delta GSTs. In D. melanogaster, the DNP-GSH affinity-purified GSTs showed mostly the presence of Epsilon and Omega GSTs whereas in L. cuprina no Omega GSTs were detected. In both species, the migration pattern of Delta GST on 2D PAGE gel indicated possible post-translational modification. The results from analysis of LC-MS/MS data by the software PEAKS suggested deamidation at asparagine and glutamine residues in a limited number of the matched peptides of Delta GST. GST activity was present in all developmental stages of L. cuprina. The number of isoenzymes and their extent of expression vary as the insect develops. Delta GSTs were present in all developmental stages. The Sigma GST started expressing from the larval stage and was abundantly present in adult stage. The DNP-GSH affinity matrix purified GSTs which have been tentatively classified as Mu-like GSTs were present in egg, larvae and pupae but totally absent in adult stage. The GST families were characterised by proteomics in the main body sections of L. cuprina. Higher GST activity towards 1-chloro-2, 4-dinitrobenzene (CDNB) was found in the thorax (65.2 %) followed by the abdomen (19.6%) and the head (15.2%). The cytosolic GSTs of a resistant strain (PY81) of L. cuprina had significantly higher (2.26- and 2.6- fold) activity than the susceptible strains (NSW and CSIRO) towards CDNB and 2, 3-dichloro, 4-nitrobenzene (DCNB) respectively. The proteomic analysis of DNP-GSH purified extract from susceptible and resistant strains showed quantitatively higher expression of GSTs on 2D PAGE gel of the PY81 strain. The in vitro interaction of purified GSTs… Advisors/Committee Members: Clark, Alan, Teesdale-Spittle, Paul.

Subjects/Keywords: Glutathione; Transferases; Lucilia cuprina; Proteomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pal, R. (2010). Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/1541

Chicago Manual of Style (16^th Edition):

Pal, Ramavati. “Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina.” 2010. Doctoral Dissertation, Victoria University of Wellington. Accessed August 23, 2019. http://hdl.handle.net/10063/1541.

MLA Handbook (7^th Edition):

Pal, Ramavati. “Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina.” 2010. Web. 23 Aug 2019.

Vancouver:

Pal R. Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2010. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/10063/1541.

Council of Science Editors:

Pal R. Proteomic Analysis of Glutathione Transferases from Lucilia Cuprina. [Doctoral Dissertation]. Victoria University of Wellington; 2010. Available from: http://hdl.handle.net/10063/1541

22. Lovejoy, Richard Perry. The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide.

Degree: Chemistry, 1934, University of Massachusetts

URL: https://scholarworks.umass.edu/theses/1736

Subjects/Keywords: Glutathione; Sulphur

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APA (6^th Edition):

Lovejoy, R. P. (1934). The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide. (Thesis). University of Massachusetts. Retrieved from https://scholarworks.umass.edu/theses/1736

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lovejoy, Richard Perry. “The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide.” 1934. Thesis, University of Massachusetts. Accessed August 23, 2019. https://scholarworks.umass.edu/theses/1736.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lovejoy, Richard Perry. “The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide.” 1934. Web. 23 Aug 2019.

Vancouver:

Lovejoy RP. The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide. [Internet] [Thesis]. University of Massachusetts; 1934. [cited 2019 Aug 23]. Available from: https://scholarworks.umass.edu/theses/1736.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lovejoy RP. The occurrence of glutathione in the foliage of horticultural plants, and its relation to the use of sulfur as a fungicide. [Thesis]. University of Massachusetts; 1934. Available from: https://scholarworks.umass.edu/theses/1736

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Rochester

23. Marchan, Rosemarie. Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis.

Degree: PhD, 2009, University of Rochester

URL: http://hdl.handle.net/1802/6523

► Glutathione (GSH) is important for normal cellular functions and recent observations suggest that GSH also plays a key role in apoptotic cell death. During apoptosis,… (more)

▼ Glutathione (GSH) is important for normal cellular functions and recent observations suggest that GSH also plays a key role in apoptotic cell death. During apoptosis, intracellular GSH is rapidly depleted due to its enhanced export from cells. The precise mechanism behind the GSH efflux and its significance to the apoptotic process are not understood. Although loss of intracellular GSH may simply be a result of apoptosis, recent studies suggest that GSH depletion is required for the activation of specific signaling pathways, including those mediated by reactive oxygen species. The overall goal of the presented work is to determine whether members of the multidrug resistance-associated protein family (MRP/ABCC) mediate GSH release during apoptosis, and whether this release is important for the progression of apoptosis. Fas antibody and staurosporine were used to induce apoptosis in three cell types: Jurkat cells, Raji cells, and HEK293 cells overexpressing MRP1. Apoptotic GSH release in Jurkat cells was associated with an increase in apoptosis because inhibiting GSH release decreased apoptosis. Conversely, Raji cells did not release GSH nor externalize phosphatidylserine when treated with Fas antibody or staurosporine. Additionally, apoptosis was slower in Raji cells when compared to the Jurkat cells, suggesting that GSH release is important for the progression of apoptosis and may be associated with phosphatidylserine externalization. MRP expression and function were measured in Jurkat and Raji cells to further characterize the role of MRPs in GSH transport. Although both cell lines expressed similar levels of MRPs, they were not functional in the Raji cells. MRP1 vii was further analyzed because it is implicated in GSH transport and is ubiquitously expressed in mammalian cells. Decreasing MRP1 expression in Jurkat cells inhibited both basal and apoptotic GSH release, providing direct evidence for MRP1 in mediating GSH transport. Furthermore, HEK293 cells overexpressing MRP1 had elevated basal and apoptotic GSH release; however, apoptosis was not increased in these cells, an effect that is most likely explained by an enhanced rate of GSH synthesis in these cells. Overall, these studies support a role for the MRPs, more specifically MRP1, in GSH release and indicate that GSH release is important for the progression of apoptosis.

Subjects/Keywords: Glutathione; Multidrug resistance; Apoptosis

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APA (6^th Edition):

Marchan, R. (2009). Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/6523

Chicago Manual of Style (16^th Edition):

Marchan, Rosemarie. “Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis.” 2009. Doctoral Dissertation, University of Rochester. Accessed August 23, 2019. http://hdl.handle.net/1802/6523.

MLA Handbook (7^th Edition):

Marchan, Rosemarie. “Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis.” 2009. Web. 23 Aug 2019.

Vancouver:

Marchan R. Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1802/6523.

Council of Science Editors:

Marchan R. Role of the multidrug resistance-associated proteins (MRP/ABCC) in glutathione transport during apoptosis. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/6523

University of Rochester

24. Bushman, Jared S.; Mayer-Proschel, Margot. Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia.

Degree: PhD, 2009, University of Rochester

URL: http://hdl.handle.net/1802/6602

► The neurodegenerative features of the autosomal recessive disorder ataxia telangiectasia (AT) show a remarkable degree of region specificity in their appearance. Accumulation of oxidative damage… (more)

Subjects/Keywords: Glutathione; Ataxia; Astrocytes; Purkinse; Redox

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APA (6^th Edition):

Bushman, Jared S.; Mayer-Proschel, M. (2009). Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/6602

Chicago Manual of Style (16^th Edition):

Bushman, Jared S.; Mayer-Proschel, Margot. “Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia.” 2009. Doctoral Dissertation, University of Rochester. Accessed August 23, 2019. http://hdl.handle.net/1802/6602.

MLA Handbook (7^th Edition):

Bushman, Jared S.; Mayer-Proschel, Margot. “Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia.” 2009. Web. 23 Aug 2019.

Vancouver:

Bushman, Jared S.; Mayer-Proschel M. Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1802/6602.

Council of Science Editors:

Bushman, Jared S.; Mayer-Proschel M. Astrocyte properties modulate neuronal dysfunction in ataxia telangiectasia. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/6602

University of Rochester

25. Krance, Suzanne M. Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation.

Degree: PhD, 2009, University of Rochester

URL: http://hdl.handle.net/1802/6613

► The tripeptide glutathione (L-γ-glutamyl-L-cysteinylglycine; GSH), is the most abundant intracellular thiol and functions as a major regulator of the intracellular redox state. Imbalances in GSH… (more)

▼ The tripeptide glutathione (L-γ-glutamyl-L-cysteinylglycine; GSH), is the most abundant intracellular thiol and functions as a major regulator of the intracellular redox state. Imbalances in GSH homeostasis are associated with a wide array of disease states, and are implicated in the aging process. For years, research on GSH has focused on its role in detoxification, however a renewed interest in the molecule has focused on its role in major cell processes, including cell differentiation. A number of studies have described GSH status in differentiated cells, however limited information is available concerning GSH status during the process. In addition, many seemingly contradictory reports have been made concerning the effects of altering GSH levels on differentiation outcomes. Studies in this thesis were designed to elucidate GSH status during terminal differentiation, and to determine how altering GSH during the process affects differentiation. It was hypothesized that GSH status is dynamic during terminal differentiation, and that depleting levels of this tripeptide modulates differentiation outcomes. In order to test this hypothesis, HL-60 cells were induced to differentiate into either neutrophil or monocyte-like cells, and GSH levels as well as differentiation marker expression were assessed during the process. Methods used to assess differentiation include flow cytometric analysis of CD11b and CD71 cell surface marker expression and cell cycle distribution, and the NBT reduction assay, used to measure superoxide anion production capability. These studies demonstrated that a transient increase in glutathione is a feature of HL-60 differentiation, and that this phenomenon coincides with increased NBT reduction capacity, but precedes alterations in cell surface marker expression and proliferation. When GSH was depleted during induced HL-60 maturation, differentiation marker expression was enhanced. In contrast, thiol supplementation diminished differentiation marker expression. GSH depletion also increased spontaneous maturation in uninduced HL-60 cells. In summary, these data demonstrate that glutathione levels are dynamic during HL-60 differentiation, and that altering intracellular glutathione content during the process modulates differentiation outcomes. Taken together, these findings suggest that glutathione status plays an important role in modulating differentiation outcomes, and thus may contribute to conditions, and disease states characterized by abnormal differentiation.

Subjects/Keywords: THIOL; Glutathione; Differentiation; HL-60

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APA (6^th Edition):

Krance, S. M. (2009). Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/6613

Chicago Manual of Style (16^th Edition):

Krance, Suzanne M. “Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation.” 2009. Doctoral Dissertation, University of Rochester. Accessed August 23, 2019. http://hdl.handle.net/1802/6613.

MLA Handbook (7^th Edition):

Krance, Suzanne M. “Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation.” 2009. Web. 23 Aug 2019.

Vancouver:

Krance SM. Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation. [Internet] [Doctoral dissertation]. University of Rochester; 2009. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/1802/6613.

Council of Science Editors:

Krance SM. Glutathione homeostasis during terminal human leukemia-60 (HL-60) cell differentiation. [Doctoral Dissertation]. University of Rochester; 2009. Available from: http://hdl.handle.net/1802/6613

University of Florida

26. Xu, Changjie. The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity.

Degree: PhD, Nutritional Sciences, 2017, University of Florida

URL: http://ufdc.ufl.edu/UFE0051738

► Obesity is global health issue. In the United States, more than one-third of adults are obese. Obesity induces chronic systemic inflammation and increasing obesity positively… (more)

Subjects/Keywords: garlic – glutathione – inflammation – obesity

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APA (6^th Edition):

Xu, C. (2017). The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity. (Doctoral Dissertation). University of Florida. Retrieved from http://ufdc.ufl.edu/UFE0051738

Chicago Manual of Style (16^th Edition):

Xu, Changjie. “The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity.” 2017. Doctoral Dissertation, University of Florida. Accessed August 23, 2019. http://ufdc.ufl.edu/UFE0051738.

MLA Handbook (7^th Edition):

Xu, Changjie. “The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity.” 2017. Web. 23 Aug 2019.

Vancouver:

Xu C. The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity. [Internet] [Doctoral dissertation]. University of Florida; 2017. [cited 2019 Aug 23]. Available from: http://ufdc.ufl.edu/UFE0051738.

Council of Science Editors:

Xu C. The Effects and Mechanisms of Consuming Sulfur-Containing Dietary Supplements on Chronic Inflammation in Adults with Obesity. [Doctoral Dissertation]. University of Florida; 2017. Available from: http://ufdc.ufl.edu/UFE0051738

University of Cape Town

27. Corrigall, Anne Vint. Human glutathione S-transferases : characterization, tissue distribution and kinetic studies.

Degree: Image, UCT/MRC Liver Research Centre, 1988, University of Cape Town

URL: http://hdl.handle.net/11427/27205

► In this study the purification of human basic and near-neutral liver, and human basic and acidic lung glutathione S-transferases (GSH S-T) was undertaken. Purification of… (more)

▼ In this study the purification of human basic and near-neutral liver, and human basic and acidic lung glutathione S-transferases (GSH S-T) was undertaken. Purification of the basic and near-neutral GSH S-T was achieved using a combination of affinity chromatography, chromatofocusing and immunoaffinity chromatography. Affinity and ion exchange chromatography were employed in the purification of the basic and acidic lung forms. The purified proteins had similar physicochemical characteristics to the GSH S-T purified by others. The binding of 1-chloro-2,4-dinitrobenzene (CDNB) to the 3 classes of human GSH S-T, viz. basic, near-neutral and acidic and the effects of such binding, if any, were examined. Human acidic lung GSH S-T is irreversibly inactivated by CDNB in the absence of the co-substrate glutathione (GSH). The time-dependent inactivation is pseudo-first order and demonstrates saturation kinetics, suggesting that inactivation occurs from an EI complex. GSH protects the enzyme against CDNB inactivation. In contrast, the basic and near-neutral GSH S-T are not significantly inactivated by CDNB. Incubation with [¹⁴C]-CDNB indicated covalent binding to all 3 classes of GSH S-T. When the basic and acidic GSH S-T were incubated with [¹⁴C]-CDNB and GSH, cleaved with cyanogen bromide, and chromatographed by HPLC, a single peptide fraction was found to be labelled in both classes. Incubation in the absence of GSH yielded 1 and 2 additional labelled peptide fractions for the basic and acidic transferases, respectively. These results suggest that while CDNB arylates all 3 classes of human GSH S-T, only the acidic GSH S-T possesses a specific GSH-sensitive CDNB binding site, which when occupied leads to time-dependent inactivation of the enzyme. The tissue distribution and localization of the 3 classes of human GSH S-T in normal and tumour tissue was examined. Antibodies to representatives of the 3 classes were raised in rabbits, and radial immunodiffusion employed to quantitate their concentrations in the cytosol of 18 organs from 9 individuals. The data provide the first direct, quantitative evidence for the inter-individual and inter-organ variation suggested by earlier workers. The absence of the near-neutral GSH S-T in 5 of the 9 individuals studied confirms an earlier suggestion of a "null" allele for this transferase. Basic and acidic GSH S-T (apart from in a single liver), were always present. Near-neutral GSH S-T, when present, were found in all tissues examined. The marked inter-organ and inter-individual variation observed in this study may explain individual and organ susceptibility to drugs, toxins and carcinogens. The immunohistochemical localization of the 3 classes of GSH S-T reveals important differences in their localization, and may provide insight into their functions in various organs and tissues. Advisors/Committee Members: Kirsch, Ralph E (advisor).

Subjects/Keywords: Glutathione transferase; Glutathione transferases

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APA (6^th Edition):

Corrigall, A. V. (1988). Human glutathione S-transferases : characterization, tissue distribution and kinetic studies. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/27205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Corrigall, Anne Vint. “Human glutathione S-transferases : characterization, tissue distribution and kinetic studies.” 1988. Thesis, University of Cape Town. Accessed August 23, 2019. http://hdl.handle.net/11427/27205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Corrigall, Anne Vint. “Human glutathione S-transferases : characterization, tissue distribution and kinetic studies.” 1988. Web. 23 Aug 2019.

Vancouver:

Corrigall AV. Human glutathione S-transferases : characterization, tissue distribution and kinetic studies. [Internet] [Thesis]. University of Cape Town; 1988. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/11427/27205.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Corrigall AV. Human glutathione S-transferases : characterization, tissue distribution and kinetic studies. [Thesis]. University of Cape Town; 1988. Available from: http://hdl.handle.net/11427/27205

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

28. Goold, Richard David. The glutathione S-transferases : kinetics, binding and inhibition.

Degree: Image, Division of Medical Biochemistry & Structural Biology, 1989, University of Cape Town

URL: http://hdl.handle.net/11427/27175

► The glutathione S-transferases are a group of enzymes which catalyse the conjugation of reduced glutathione with a variety of electrophilic molecules, and they are therefore… (more)

Subjects/Keywords: Medical Biochemistry; Glutathione transferase; Glutathione transferases; Glutathione transferases - Analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goold, R. D. (1989). The glutathione S-transferases : kinetics, binding and inhibition. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/27175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goold, Richard David. “The glutathione S-transferases : kinetics, binding and inhibition.” 1989. Thesis, University of Cape Town. Accessed August 23, 2019. http://hdl.handle.net/11427/27175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goold, Richard David. “The glutathione S-transferases : kinetics, binding and inhibition.” 1989. Web. 23 Aug 2019.

Vancouver:

Goold RD. The glutathione S-transferases : kinetics, binding and inhibition. [Internet] [Thesis]. University of Cape Town; 1989. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/11427/27175.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goold RD. The glutathione S-transferases : kinetics, binding and inhibition. [Thesis]. University of Cape Town; 1989. Available from: http://hdl.handle.net/11427/27175

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Abdu, Habibu U. Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides .

Degree: 2015, Abertay U

URL: http://hdl.handle.net/10373/2479

► Malaria remains a serious public health challenge in the tropical world, with 584,000 deaths globally in 2013, of which 90% occurred in Africa, and mostly… (more)

▼ Malaria remains a serious public health challenge in the tropical world, with 584,000 deaths globally in 2013, of which 90% occurred in Africa, and mostly in pregnant women and children under the age of five. Anopheles gambiae (An. gambiae) is the principal malaria vector in Africa, where vector control measures involve the use of insecticides in the forms of long-lasting insecticide-treated nets (LLINs) and indoor residual spraying (IRS). The development of insecticides resistance mitigates these approaches. Glutathione (GSH) is widely distributed among all living organisms, and is associated with detoxification pathways, especially the Glutathione S-transferases (GSTs). Its direct involvement and relevance in insecticide resistance in An. gambiae has not been determined. Thus, this work examines the contribution of GSH, its biosynthetic genes (GCLM, GCLC) and their possible transcriptional regulator Nrf2 in insecticide resistance in An. gambiae sampled from agricultural setting (areas of intensive agriculture) and residential setting (domestic area). Bioinformatics analysis, W.H.O. adult susceptibility bioassays and molecular techniques were employed to investigate. Total RNA was first isolated from the adults An. gambiae mosquitoes raised from agricultural and residential field-caught larvae which had been either challenged or unchallenged with insecticides. Semi-quantitative RT-PCR using gel image densitometry was used to determine the expression levels of GCLM, GCLC genes and Nrf2. Bioinformatics’ results established the presence of putative AGAP010259 (AhR) and AGAP005300 (Nf2e1) transcription factor binding sites in An. gambiae GCLC and GCLM promoters in silico. An. gambiae s.l. studied here were highly resistant to DDT and permethrin but less resistant to bendiocarb. Both knockdown resistance (kdr) mutation variants L1014S and L1014F that confers resistance to pyrethroid insecticides were identified in both An. coluzzii and An. arabiensis sampled from northern Nigeria. The L1014F was much associated with An. coluzzii. A significant positive correlation (P=0.04) between the frequency of the L1014F point mutation and resistance to DDT and permethrin was observed. However, a weak or non-significant correlation (P=0.772) between the frequency of the L1014S point mutation and resistance was also found. L1014S and L1014F mutations co-occurred in both agricultural and residential settings with high frequencies. However, the frequencies of the two mutations were greater in the agricultural settings than in the residential settings. The levels of total, reduced and oxidized GSH were significantly higher in mosquitoes from agricultural sites than those from residential sites. Increased oxidized GSH levels appears to correlate with higher DDT resistance. The expression levels of GCLM, GCLC and Nrf2 were also significantly up-regulated in adults An. gambiae raised from agricultural and residential field-caught larvae when challenged with insecticide. However, there was higher constitutive expression of GCLM, GCLC and… Advisors/Committee Members: Deeni, Yusuf,Y (advisor).

Subjects/Keywords: Anopheles gambiae; GCLM; Glutathione; Insecticide; Nigeria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Abdu, H. U. (2015). Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides . (Thesis). Abertay U. Retrieved from http://hdl.handle.net/10373/2479

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Abdu, Habibu U. “Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides .” 2015. Thesis, Abertay U. Accessed August 23, 2019. http://hdl.handle.net/10373/2479.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Abdu, Habibu U. “Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides .” 2015. Web. 23 Aug 2019.

Vancouver:

Abdu HU. Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides . [Internet] [Thesis]. Abertay U; 2015. [cited 2019 Aug 23]. Available from: http://hdl.handle.net/10373/2479.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Abdu HU. Investigating the role of glutathione and glutathione biosynthetic genes in the adaptation of Anopheles gambiae to insecticides . [Thesis]. Abertay U; 2015. Available from: http://hdl.handle.net/10373/2479

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Durham University

30. Grundy, Nicholas Matthew. Protein S-thiolation and oxidative stress in plants.

Degree: PhD, 2002, Durham University

URL: http://etheses.dur.ac.uk/3950/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268725

► The tripeptides glutathione (GSH; γglutamyl-cysteinyl-glycine) and homoglutathione (hGSH; γglutamyl-cysteinyl-β-alanine) are abundant cytosolic tripeptides in legumes. The reactive cysteinyl sulphydryl group enables GSH or hGSH to… (more)

▼ The tripeptides glutathione (GSH; γglutamyl-cysteinyl-glycine) and homoglutathione (hGSH; γglutamyl-cysteinyl-β-alanine) are abundant cytosolic tripeptides in legumes. The reactive cysteinyl sulphydryl group enables GSH or hGSH to act as the major cellular redox buffer through the formation of disulphides with other GSH/hGSH molecules. GSH can also form disulphides with cysteinyl groups within proteins, which is termed 5-thiolation, a reversible modification, protecting proteins from irreversible inactivation of thiol residues, as well as being important in regulating protein activity. Following treatment with fungal cell wall elicitors, plant cells produce reactive oxygen species (ROS) which results in cellular oxidative stress. In animal cells ROS generation induces antioxidant defences which include the accumulation of glutathione (GSH) and the formation of mixed disulphides between proteins and GSH. It was hypothesised that following treatment with a fringal elicitor, plant cells also thiolate proteins. It was of interest to determine how protein thiolation changed in response to changes in thiol metabolism known to occur during elicitation, as well as identifying proteins which underwent this modification. Using cell cultures of alfalfa (Medicago saliva L.), a leguminous plant containing both GSH and hGSH, changes in thiol content upon treatment with a fungal cell wall preparation elicitor were determined. By inhibiting protein synthesis and labelling the thiol pools with L-[(³⁵)S]cysteine, the degree and rate of protein mixed disulphide formation could be monitored in-vivo. To induce the elicitation response, alfalfa cell cultures were treated with a fungal cell-wall elicitor. Following elicitor treatment GSH, but not hGSH, was found to accumulate, with an associated increase in GSH, but not hGSH, forming mixed disulphide with protein. In order to use proteomic tools to identify thiolated proteins, the oxidative stress response in cell cultures of Arabidopsis, a GSH containing species, was then characterised. The level of protein-bound GSH was found to increase following treatment of cell cultures with the oxidant tert-hutyl hydroperoxide and this was associated with changes in cellular thiols. When proteins S-thiolated either in-vivo, or in-vitro, with [(³⁵)S]-GSH were resolved by SDS-PAGE under non-reducing conditions, a large number of radiolabelled polypeptides were identified in oxidatively stressed preparations. Testing the hypothesis that GSH-dependent enzymes may undergo S-thiolation, proteins which bound GSH were isolated from Arabidopsis using GSH-afFinity chromatography. A number of 30 kDa polypeptides were isolated and found to be S-thiolated under oxidative conditions in-vitro. Several of these were subsequently identified, notably members of the glutathione transferase (GST) superfamily. Representative recombinant GSTs from Arabidopsis, maize and soybean were expressed, Violated in-vitro and the effect on activity determined. Several thiolatable GSTs were identified from Arabidopsis, notably the…

Subjects/Keywords: 571; Glutathione transferase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Grundy, N. M. (2002). Protein S-thiolation and oxidative stress in plants. (Doctoral Dissertation). Durham University. Retrieved from http://etheses.dur.ac.uk/3950/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268725

Chicago Manual of Style (16^th Edition):

Grundy, Nicholas Matthew. “Protein S-thiolation and oxidative stress in plants.” 2002. Doctoral Dissertation, Durham University. Accessed August 23, 2019. http://etheses.dur.ac.uk/3950/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268725.

MLA Handbook (7^th Edition):

Grundy, Nicholas Matthew. “Protein S-thiolation and oxidative stress in plants.” 2002. Web. 23 Aug 2019.

Vancouver:

Grundy NM. Protein S-thiolation and oxidative stress in plants. [Internet] [Doctoral dissertation]. Durham University; 2002. [cited 2019 Aug 23]. Available from: http://etheses.dur.ac.uk/3950/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268725.

Council of Science Editors:

Grundy NM. Protein S-thiolation and oxidative stress in plants. [Doctoral Dissertation]. Durham University; 2002. Available from: http://etheses.dur.ac.uk/3950/ ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268725

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