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You searched for subject:(gingival epithelium). Showing records 1 – 3 of 3 total matches.

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The Ohio State University

1. Lai, Pin-Chuang. Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations.

Degree: PhD, Oral Biology, 2015, The Ohio State University

Bacterial plaque is a primary etiological factor of periodontitis, and a specific group of pathogens is strongly associated with this destructive inflammatory entity. Although conventional periodontal scaling and root planing usually halts the disease process, the loss of periodontal attachment continues in some patients. Aggressive and recurrent forms of periodontitis are associated with persistent infection by Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. These pathogens can invade gingival epithelium and fibroblasts, allowing them to evade the host immune system and re-colonize pockets after debridement. Although the use of adjunctive systemic antibiotics to treat invasive infection by these pathogens is recommended, an agreement on the most effective regimen has not been reached. Azithromycin is not commonly used in periodontal therapy. Its favorable properties include inhibition of a broad spectrum of periodontal pathogens, a high volume of distribution, a long half-life, and relatively low incidence of side effects. Studies have indicated that human cells take up azithromycin through active transport. In this project, we hypothesize that gingival epithelial cells, fibroblasts, and neutrophils possess active transporters that accumulate azithromycin. These transporters may enhance and sustain levels of azithromycin in cellular compartments of gingiva and in gingival crevicular fluid. The accumulated azithromycin may facilitate elimination of intracellular periodontal pathogens. The chapters of this dissertation are 1) to study the mechanism by which azithromycin is transported by cells in the gingiva, and 2) to evaluate the benefits of this transport system with regards to pharmacokinetics and antimicrobial activity against intracellular A. actinomycetemcomitans and P. gingivalis. The characterization of azithromycin transport was conducted with radiolabeled azithromycin. To study the mechanism of transport, agents with similar molecular features were added to test their effects on azithromycin transport. To study the pharmacological benefits of azithromycin transport, human subjects with clinically healthy periodontal tissues were recruited. Following systemic administration of azithromycin, serum and gingival crevicular fluid were sampled and the content of azithromycin was determined by agar diffusion bioassays. The intracellular antimicrobial activity of azithromycin was evaluated in gingival epithelial cells, fibroblasts, and neutrophils. Periodontal pathogens were first internalized in these cells, and the surviving colony-forming units after antibiotic treatment were measured by growing the cellular lysates on appropriate agar plates. Our data suggest that gingival epithelial cells, fibroblasts, and neutrophils possess saturable, concentrative active transport systems for azithromycin that are shared with organic cations. The transport systems lead to high degrees of intracellular accumulation in gingival epithelial cells, fibroblasts, and neutrophils, with cellular/extracellular… Advisors/Committee Members: Walters, John (Advisor).

Subjects/Keywords: Dentistry; Pharmacology; antimicrobial agents; macrolide; periodontitis; pharmacokinetics; invasive microorganisms; neutrophil; gingival fibroblast; gingival epithelium

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APA (6th Edition):

Lai, P. (2015). Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1448876943

Chicago Manual of Style (16th Edition):

Lai, Pin-Chuang. “Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations.” 2015. Doctoral Dissertation, The Ohio State University. Accessed May 08, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1448876943.

MLA Handbook (7th Edition):

Lai, Pin-Chuang. “Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations.” 2015. Web. 08 May 2021.

Vancouver:

Lai P. Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations. [Internet] [Doctoral dissertation]. The Ohio State University; 2015. [cited 2021 May 08]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1448876943.

Council of Science Editors:

Lai P. Azithromycin in periodontal therapy: pharmacokinetic and mechanistic investigations. [Doctoral Dissertation]. The Ohio State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1448876943


University of Western Ontario

2. Michelsons, Sarah. Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts.

Degree: 2014, University of Western Ontario

Guided tissue regeneration (GTR) to regenerate periodontal tissue involves placement of a cell-occlusive barrier membrane functionally excluding the gingiva and associated oral epithelium from the periodontal defect. Gingival connective tissue (CT) contains a rich vascular plexus and is a source of progenitor cells which could contribute to periodontal regeneration. We propose the use of a novel biodegradable and bioactive electrospun fibrous polycaprolactone (PCL) scaffold loaded with microspheres releasing basic fibroblast growth factor (bFGF) to promote gingival CT growth while maintaining a barrier to the oral epithelium. Scaffolds supported human gingival fibroblast proliferation and mesenchymal cell infiltration in a bFGF dose dependent manner. Oral epithelial cells were excluded from the interior of the scaffolds. Scaffold treatment during early healing of rat gingival wounds showed good biocompatibility. This study suggests that PCL electrospun scaffolds loaded with bFGF microspheres represent a promising alternative to the current generation of GTR barrier membranes.

Subjects/Keywords: Gingival fibroblasts; Oral epithelium; Polycaprolactone; Electrospun scaffold; Basic fibroblast growth factor; Guided tissue regeneration; Medical Cell Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Michelsons, S. (2014). Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/2607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Michelsons, Sarah. “Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts.” 2014. Thesis, University of Western Ontario. Accessed May 08, 2021. https://ir.lib.uwo.ca/etd/2607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Michelsons, Sarah. “Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts.” 2014. Web. 08 May 2021.

Vancouver:

Michelsons S. Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts. [Internet] [Thesis]. University of Western Ontario; 2014. [cited 2021 May 08]. Available from: https://ir.lib.uwo.ca/etd/2607.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Michelsons S. Influence of Polycaprolactone and Basic Fibroblast Growth Factor on Gingival Fibroblasts. [Thesis]. University of Western Ontario; 2014. Available from: https://ir.lib.uwo.ca/etd/2607

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Hatayama, Takahide. Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold .

Degree: 2019, Kyoto University

Subjects/Keywords: Regeneration; gingival tissue; in situ tissue engineering; collagen; epithelium and submucosa

Page 1 Page 2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hatayama, T. (2019). Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/243271

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hatayama, Takahide. “Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold .” 2019. Thesis, Kyoto University. Accessed May 08, 2021. http://hdl.handle.net/2433/243271.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hatayama, Takahide. “Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold .” 2019. Web. 08 May 2021.

Vancouver:

Hatayama T. Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold . [Internet] [Thesis]. Kyoto University; 2019. [cited 2021 May 08]. Available from: http://hdl.handle.net/2433/243271.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hatayama T. Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold . [Thesis]. Kyoto University; 2019. Available from: http://hdl.handle.net/2433/243271

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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