subject:(genetic engineering)

1. Pérez Álvarez, Lucía. Metabolic engineering and genome editing in rice.

Degree: Departament de Producció Vegetal i Ciència Forestal, 2018, Universitat de Lleida

► My research program used rice as an experimental model to address fundamental bottlenecks and mechanisms limiting the transition from metabolic engineering to synthetic biology in… (more)

▼ My research program used rice as an experimental model to address fundamental bottlenecks and mechanisms limiting the transition from metabolic engineering to synthetic biology in plants. I concentrated on an in depth molecular and biochemical characterization of plants I generated in two distinct, yet interrelated sets of research lines. In the first set of experiments I addressed the hypothesis that by knocking out specific genes in a primary metabolic pathway, starch biosynthesis, resulting mutant plants might exhibit phenotypes conducive to synthetic biology applications in terms of redirecting flux and limiting biosynthetic precursors to specific secondary metabolic pathways. In this context I used CRISPR/Cas9 to create two heterozygous mutants, one with a severely truncated and non-functional cytosolic glucose-1-phosphate adenylyl transferase (AGPase) and the other with a C-terminal structural modification causing a partial loss of activity. Unexpectedly, both mutants exhibited depletion of starch in the leaves and a corresponding increase in the level of soluble sugars. This reflected the unanticipated expression of both OsAPL2 and OsAPS2b in the leaves, generating a complete ectopic AGPase in the leaf cytosol, and a corresponding decrease in the expression of the plastidial small subunit OsAPS2a that was only partially complemented by an increase in the expression of OsAPS1. In a subsequent set of experiments along similar lines I investigated the broader effects of mutations in an additional starch biosynthetic gene, granule bound starch synthase (GBBS, waxy). I used CRISPR/Cas9 to introduce a range of mutations with different effects in this specific gene. All mutations I recovered reduced but did not abolish GBSS activity in seeds due to partial compensation caused by the ectopic upregulation of GBSSII. The GBSS activity in the mutants was 61–71% of wild-type levels, but the amylose content nevertheless declined to 8–12% in heterozygous seeds and to as low as 5% in homozygous seeds, accompanied by abnormal cellular organization in the aleurone layer and amorphous starch grain structures. Almost every starch pathway gene was impacted at different degrees in leaves and seeds. These gene expression changes resulted in changes in AGPase and sucrose synthase activity that explained the corresponding levels of starch and soluble sugars. The second line of my program focused on the engineering of an ectopic MVA pathway in rice plastids in order to investigate the hypothesis that by reconstituting such an ectopic pathway the strict regulation of the native MVA pathway might be relieved to a certain degree in turns increasing the pool of essential terpenoid precursors. Results indicated a profound increase in the levels of fatty acids, lutein and tocopherol, a decrease in squalene levels and similar levels of sterols. My results set the stage for further experiments to ascertain whether germplasm I created and characterized, can serve as a basis for more complex metabolic engineering and synthetic biology… Advisors/Committee Members: [email protected] (authoremail), false (authoremailshow), Christou, Paul (director), Villorbina Noguera, Gemma (director).

Subjects/Keywords: Enginyeria genetica; Edicio del genoma; Mido; Ingenieria genetica; Edicino del genoma; Almidon; Genetic engineering; Genome editing; Starch; Bioquímica i Biologia Molecular; 577

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pérez Álvarez, L. (2018). Metabolic engineering and genome editing in rice. (Thesis). Universitat de Lleida. Retrieved from http://hdl.handle.net/10803/665272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pérez Álvarez, Lucía. “Metabolic engineering and genome editing in rice.” 2018. Thesis, Universitat de Lleida. Accessed January 20, 2021. http://hdl.handle.net/10803/665272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pérez Álvarez, Lucía. “Metabolic engineering and genome editing in rice.” 2018. Web. 20 Jan 2021.

Vancouver:

Pérez Álvarez L. Metabolic engineering and genome editing in rice. [Internet] [Thesis]. Universitat de Lleida; 2018. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10803/665272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pérez Álvarez L. Metabolic engineering and genome editing in rice. [Thesis]. Universitat de Lleida; 2018. Available from: http://hdl.handle.net/10803/665272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Cappadonna, Fulvio Antonio. Application of exact and approximate optimization methods to novel scheduling problems.

Degree: 2014, Università degli Studi di Catania

URL: http://hdl.handle.net/10761/1482

► The application of exact and heuristic optimization techniques to scheduling problems pertaining to production processes has been widely investigated over the last decades by the… (more)

▼ The application of exact and heuristic optimization techniques to scheduling problems pertaining to production processes has been widely investigated over the last decades by the relevant scientific literature in the field of industrial systems design and analysis. In general, the term scheduling is used with reference to the allocation of resources to tasks over time, so to execute all planned activities according to a given performance objective (minimization of costs, minimization of production time, due dates fulfilment, etc.). Even though basic scheduling problems have been effectively solved long time ago, this topic still remains attractive for expert and practitioners, as the technological innovation of production processes and the need for effective planning activities emerging from new sectors still set new frontiers to the scheduling optimization research. The aim of the present Thesis is to investigate three scheduling problems that have not been addressed yet by the literature, even though they have a clear correspondence to real-world manufacturing environments. The first problem addressed is the minimization of makespan in an unrelated parallel machine system with sequence-dependent setup times and limited human resources. Workers are needed to perform setup operations before each job is processed; they are supposed to be a critical resource as their number is assumed to be lower than the number of workstations available in the production shop. In addition, each worker is characterized by a provided skill level, which affects the time required for completing setup operations. Firstly, a Mixed Integer Linear Programming (MILP) model suitable for tackling small instances of the problem in hand is illustrated. Then, an optimization framework based on Genetic Algorithms (GAs) is presented with the aim of effectively addressing larger test cases. Three different procedures are proposed, namely a permutation based GA, a multi-encoding GA, and a hybrid GA. An extensive benchmark including both small-and large-sized instances is taken as reference for both calibration and comparison of the proposed methods, which have been performed by means of ANOVA analysis. The second problem addressed is the minimization of makespan in a Flow Shop Sequence Dependent Group Scheduling (FSDGS) problem entailing the worker allocation issue. As first, a Mixed Integer Linear Programming (MILP) formulation for the problem is given. Then, a well-known benchmark arisen from literature is adopted for carrying out an extensive comparison campaign among three specifically developed metaheuristic methods based on a GA framework. Afterwards, the best procedure among those tested is compared with a well-performing algorithm recently proposed in the field of FSDGS problems. The third problem addressed is the minimization of makespan in a hybrid flow shop inspired to a truly observed micro-electronics manufacturing environment, is illustrated. Overlap between jobs, waiting time limit of jobs within inter-stage buffers as well as…

Subjects/Keywords: Area 09 - Ingegneria industriale e dell'informazione; scheduling, optimization, linear programming, metaheuristics, genetic algorithms

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cappadonna, F. A. (2014). Application of exact and approximate optimization methods to novel scheduling problems. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/1482

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cappadonna, Fulvio Antonio. “Application of exact and approximate optimization methods to novel scheduling problems.” 2014. Thesis, Università degli Studi di Catania. Accessed January 20, 2021. http://hdl.handle.net/10761/1482.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cappadonna, Fulvio Antonio. “Application of exact and approximate optimization methods to novel scheduling problems.” 2014. Web. 20 Jan 2021.

Vancouver:

Cappadonna FA. Application of exact and approximate optimization methods to novel scheduling problems. [Internet] [Thesis]. Università degli Studi di Catania; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10761/1482.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cappadonna FA. Application of exact and approximate optimization methods to novel scheduling problems. [Thesis]. Università degli Studi di Catania; 2014. Available from: http://hdl.handle.net/10761/1482

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

3. Bosacchi, Massimo, 1978-. The development of genetic markers for plastid transformation in higher plants.

Degree: PhD, Plant Biology, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51236/

►

The four studies that comprise my dissertation were undertaken in an effort to develop new selectable marker systems and expression elements that could make chloroplast… (more)

▼

The four studies that comprise my dissertation were undertaken in an effort to develop new selectable marker systems and expression elements that could make chloroplast transformation possible in monocot crop species. For my first project, I developed a new selectable marker system for nuclear transformation. I identified seven putative lincosamide-tolerance genes from medical studies and modified them for expression in plants. I then tested them as selectable markers for Agrobacterium mediated transformation of tobacco and generated transgenic plants with high efficiency from four of them: lnuAN2s, lnuBs, lnuDs and lnuFs. These four genes were then tested in Arabidopsis and potato. The lnuAN2s gene yielded transgenic events under lincosamide selection with similar transformation efficiencies as kanamycin and gentamicin. The performance of the new lnu marker genes makes this lincomycin selection scheme a valuable new tool for plant biologists. For my second project, I sequenced the plastid genomes of three maize lines important to transformation and breeding. The sequence was used to construct a maize-specific plastid-targeting vector and a powerful chimeric promoter for high expression in non-green tissue. I also sequenced the plastid genomes of three cytoplasmic male sterile lines, and used this information for an evolutionary study confirming a single origin and maternal co-transmission of the mitochondrial cms-T trait. For my third project I tested an herbicidal selection scheme for plastid transformation in tobacco. The glyphosate acetyltransferase (gat) gene, in combination with the chimeric maize expression element, provided protection to transplastomic cells under glyphosate selection. Because glyphosate targets a pathway that is universal in plants, the gat gene may work as a plastid marker gene across a wider range of species. For my fourth project I tested lnu genes as primary selectable marker genes for plastid transformation in tobacco. I constructed plastid-targeting vectors carrying the four efficacious lnu genes identified in my earlier study. This set of experiments did not yield transplastomic events, but I obtained five lincosamide-resistant mutants under selection in tissue culture. I sequenced the plastid rrn23 region of each line to identify the mutation responsible for the resistant phenotype.

Advisors/Committee Members: Maliga, Pal (chair), Messing, Joachim (internal member), Gallavotti, Andrea (internal member), Dong, Juan (internal member), Lutz, Kerry (outside member).

Subjects/Keywords: Plant genetic engineering; Genetic transformation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bosacchi, Massimo, 1. (2016). The development of genetic markers for plastid transformation in higher plants. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51236/

Chicago Manual of Style (16^th Edition):

Bosacchi, Massimo, 1978-. “The development of genetic markers for plastid transformation in higher plants.” 2016. Doctoral Dissertation, Rutgers University. Accessed January 20, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51236/.

MLA Handbook (7^th Edition):

Bosacchi, Massimo, 1978-. “The development of genetic markers for plastid transformation in higher plants.” 2016. Web. 20 Jan 2021.

Vancouver:

Bosacchi, Massimo 1. The development of genetic markers for plastid transformation in higher plants. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Jan 20]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51236/.

Council of Science Editors:

Bosacchi, Massimo 1. The development of genetic markers for plastid transformation in higher plants. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51236/

4. Anandan, Princia. Two phase microfluidics: new trend in model identification.

Degree: 2013, Università degli Studi di Catania

URL: http://hdl.handle.net/10761/1303

► The aim of the research is to give a proper understanding of the physical aspects involved in two-phase microfluidic systems: from the theoretical point of… (more)

▼ The aim of the research is to give a proper understanding of the physical aspects involved in two-phase microfluidic systems: from the theoretical point of view to the development of numerical solutions for the flow field by Computational Modeling (CM) issue studies devoted to standard droplet generator for separation and segmented flow, bubble and drop formation, breakup and coalescence and then with increasing complexity in large scale microfluidic processors, bubble logic i.e. bubble to bubble hydrodynamic interaction provides an on-chip process control mechanism integrating chemistry and computation. This concept has been implemented using COMSOL multiphysics 3.5a software. These show the non-linearity, gain, bistability and programmability required for scalable universal computation. Alongside experimental work, numerical tools, such as computational fluid dynamics (CFD), allow us to study and analyses the behavior of immiscible fluids within microchannels. Good understanding of these microfluidic flows provides us with leverage when utilized in chemical and biological applications. The study in the context of micro-optofluidics analysis have allowed to define in some detail the integrated system used to Thorlabs has provided for the experiments in micro-optofluidics. In particular, the characterization of the microfluidic detection devices has been used in the experimental studies of two-phase flow. In addition, the analysis carried out in the various micro-channels fixed unique features in terms of flow rates for each dimensions. The key issue is finally the study and designs of an embedded system optofluidics in micro-optics Lab-On-Chip (LOC), which allows achieving very narrow spaces in a microfluidic system, ensuring a degree of portability, which it integrates optical realizing therefore, the right balance between the two disciplines. Played as part of a global project in which design and manufacture of micro devices LOC, and experimental studies within micro-optofluidics could easily fit in a biochemical analysis of the microscopic scale of biological particles of various kinds. The dynamical model identification of the asymptotic Time signals belonging to a microfluidic Two-Phase Flow process is presented. The experimental time series are used to synchronize another system with known mathematical model but unknown parameters: the Chua s oscillator. This system has been chosen for its simple mathematical structure and for the Possibility, respect to other chaotic systems, of mapping various non-linear experimental phenomena. A genetic algorithm was exploited for parameters estimation in relation to an optimization index that takes into an account of synchronization of master (microfluidic system) and slave system (Chua s oscillator).

Subjects/Keywords: Area 09 - Ingegneria industriale e dell'informazione; Two phase flow, Universal logic computation, Genetic Algorithm, Chua's oscillator

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Anandan, P. (2013). Two phase microfluidics: new trend in model identification. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/1303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anandan, Princia. “Two phase microfluidics: new trend in model identification.” 2013. Thesis, Università degli Studi di Catania. Accessed January 20, 2021. http://hdl.handle.net/10761/1303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anandan, Princia. “Two phase microfluidics: new trend in model identification.” 2013. Web. 20 Jan 2021.

Vancouver:

Anandan P. Two phase microfluidics: new trend in model identification. [Internet] [Thesis]. Università degli Studi di Catania; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10761/1303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anandan P. Two phase microfluidics: new trend in model identification. [Thesis]. Università degli Studi di Catania; 2013. Available from: http://hdl.handle.net/10761/1303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Martínez Mariscal, Ana M. Novel molecular tools to study mycoplama cells.

Degree: Departament de Bioquímica i Biologia Molecular, 2017, Universitat Autònoma de Barcelona

URL: http://hdl.handle.net/10803/458698

► Mycoplasmas are human pathogens characterized by their reduced size and for being the free-living organisms with the smallest genomes known. Despite their apparent genomic simplicity,… (more)

Subjects/Keywords: Mycoplasma; Enginyeria genètica; Ingenieria genética; Genetic engineering; Proteïnes fluorescents; Proteínas fluorescentes; Fluorescent proteins; Ciències Experimentals; 577

…suitability for these studies, available genetic tools for engineering these microorganisms are… …3.3 Genetic tools for the study of minimal cells Mycoplasmas have evolved from gram-positive… …art available techniques, such as Cre-lox system and iCRIPR, for genome engineering in… …for genome engineering using Cre-lox technology in M. genitalium - Test the use of suicide…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martínez Mariscal, A. M. (2017). Novel molecular tools to study mycoplama cells. (Thesis). Universitat Autònoma de Barcelona. Retrieved from http://hdl.handle.net/10803/458698

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martínez Mariscal, Ana M. “Novel molecular tools to study mycoplama cells.” 2017. Thesis, Universitat Autònoma de Barcelona. Accessed January 20, 2021. http://hdl.handle.net/10803/458698.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martínez Mariscal, Ana M. “Novel molecular tools to study mycoplama cells.” 2017. Web. 20 Jan 2021.

Vancouver:

Martínez Mariscal AM. Novel molecular tools to study mycoplama cells. [Internet] [Thesis]. Universitat Autònoma de Barcelona; 2017. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10803/458698.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martínez Mariscal AM. Novel molecular tools to study mycoplama cells. [Thesis]. Universitat Autònoma de Barcelona; 2017. Available from: http://hdl.handle.net/10803/458698

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Washington University in St. Louis

6. Hoynes-O'Connor, Allison. Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.

Degree: PhD, Energy, Environmental & Chemical Engineering, 2016, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/202

► Genetic circuits enable engineers to program complex logical behaviors into living organisms. Organisms can be programmed to optimize the production of fuels and chemicals,… (more)

Subjects/Keywords: Genetic circuit; Genetic engineering; Genetic regulator; Genetic sensor; RNA regulation; Synthetic biology; Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hoynes-O'Connor, A. (2016). Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/202

Chicago Manual of Style (16^th Edition):

Hoynes-O'Connor, Allison. “Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.” 2016. Doctoral Dissertation, Washington University in St. Louis. Accessed January 20, 2021. https://openscholarship.wustl.edu/eng_etds/202.

MLA Handbook (7^th Edition):

Hoynes-O'Connor, Allison. “Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits.” 2016. Web. 20 Jan 2021.

Vancouver:

Hoynes-O'Connor A. Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2016. [cited 2021 Jan 20]. Available from: https://openscholarship.wustl.edu/eng_etds/202.

Council of Science Editors:

Hoynes-O'Connor A. Development and Characterization of Genetic Sensors and Regulators for the Construction of Environmentally-Responsive Genetic Circuits. [Doctoral Dissertation]. Washington University in St. Louis; 2016. Available from: https://openscholarship.wustl.edu/eng_etds/202

University of Tasmania

7. Pitchford, Jo(Joanne). Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures.

Degree: 2002, University of Tasmania

URL: https://eprints.utas.edu.au/21229/1/whole_PitchfordJoanne2003_thesis.pdf

► My research examines the techniques of GE and its many applications, from GE food crops and animals, xenotransplantation, stem cell research, cloning and eugenics. My… (more)

Subjects/Keywords: Genetic engineering; Genetic engineering; Genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pitchford, J. (2002). Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/21229/1/whole_PitchfordJoanne2003_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pitchford, Jo(Joanne). “Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures.” 2002. Thesis, University of Tasmania. Accessed January 20, 2021. https://eprints.utas.edu.au/21229/1/whole_PitchfordJoanne2003_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pitchford, Jo(Joanne). “Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures.” 2002. Web. 20 Jan 2021.

Vancouver:

Pitchford J. Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures. [Internet] [Thesis]. University of Tasmania; 2002. [cited 2021 Jan 20]. Available from: https://eprints.utas.edu.au/21229/1/whole_PitchfordJoanne2003_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pitchford J. Form and function : the image on the inside of the eggshell : an investigation of GE technology, its applications, the ethics involved and the possible physical and psychological impact of such procedures. [Thesis]. University of Tasmania; 2002. Available from: https://eprints.utas.edu.au/21229/1/whole_PitchfordJoanne2003_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Zambia

8. Chomba, Malulu Annette. An ethical evaluation of the Zambian Government's decision on genetically modified maize .

Degree: 2014, University of Zambia

URL: http://hdl.handle.net/123456789/3265

► This study is an ethical evaluation of Zambia’s position on genetically modified (GM) maize which was exported to Zambia by the United States of America… (more)

▼ This study is an ethical evaluation of Zambia’s position on genetically modified (GM) maize which was exported to Zambia by the United States of America (USA) through the World Food Programme as food aid. This followed food shortages after the country experienced partial droughts in 2001/2002 agriculture season, which led to widespread crop failure. After consultations with relevant groups, the Zambian government decided against the use of GM maize because of the potential harm to humans and the environment that was associated with it that called for caution in dealing with the issue.Proponents of GM maize argued that GM maize would have been an immediate solution to save people from starvation due to food shortages and that GM maize seed, if planted, would increase maize yields and subsequently food security. The opponents of GM maize argued, however, that such maize had potential harmful effects for the health of human beings and the environment and therefore supported government’s decision to reject the GM maize. The study applied five ethical approaches in evaluating the decision of the government, namely, the common good, the right to safe food, the dignity of human life, the autonomy of every nation, and the justice/fairness approach. Informed consent and the precautionary principle were also applied. The objective of the study was therefore to review the debate regarding government’s position on GM maize that was donated to Zambia by the US government through the World Food Programme to supplement food shortages experienced by communities and households affected by droughts following a drought period of the 2001/2002 agriculture season that had affected their crop yields and resulted in hunger and vulnerability.The methodology involved qualitative methods with an ethical component. Primary data was collected through formal and informal interviews with members of staff and students from major institutions of learning, research institutions, government departments and some civil society organisations. Secondary data was collected through relevant literature from books and the print media. While arguments in favour of GM maize need to be given due consideration, the arguments against were found to carry greater weight. The major findings of the study include the fact that the USA government did not seek Zambia’s permission before exporting the GM maize to Zambia, that GM maize would carry undue risks to human health and the environment, and that alternative approaches were available. The ethical evaluation concluded that the government was justified in banning GM maize. It was recommended that proven conventional plant breeding methods to develop varieties of crops with resistance/tolerance to biotic and abiotic stresses should be promoted. Despite that Zambia is not practising biotechnology in its plant breeding programmes, there are proven conventional plant breeding approaches that have and continue to be applied to develop high yielding varieties of food crops with tolerance to biotic and abiotic stresses…

Subjects/Keywords: Plant genetic Engineering; Biotechnology Policy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chomba, M. A. (2014). An ethical evaluation of the Zambian Government's decision on genetically modified maize . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/3265

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chomba, Malulu Annette. “An ethical evaluation of the Zambian Government's decision on genetically modified maize .” 2014. Thesis, University of Zambia. Accessed January 20, 2021. http://hdl.handle.net/123456789/3265.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chomba, Malulu Annette. “An ethical evaluation of the Zambian Government's decision on genetically modified maize .” 2014. Web. 20 Jan 2021.

Vancouver:

Chomba MA. An ethical evaluation of the Zambian Government's decision on genetically modified maize . [Internet] [Thesis]. University of Zambia; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/123456789/3265.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chomba MA. An ethical evaluation of the Zambian Government's decision on genetically modified maize . [Thesis]. University of Zambia; 2014. Available from: http://hdl.handle.net/123456789/3265

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Zambia

9. Musonda, Kanyanta LLoyd. Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen .

Degree: 2014, University of Zambia

URL: http://hdl.handle.net/123456789/3548

► Maize (Zea mays L) is an important multi-purpose staple cereal crop in Zambia.More than 70% of people derive their livelihood from agriculture of which maize… (more)

▼ Maize (Zea mays L) is an important multi-purpose staple cereal crop in Zambia.More than 70% of people derive their livelihood from agriculture of which maize production is the major enterprise. Maize production is affected by biotic and abiotic stresses. Most varieties developed in the Zambian National Maize Breeding Programmes have no tolerance to low nitrogen stress. This is so because variety selection is done under optimum N conditions and thereafter released to farmer conditions that are mostly Nitrogen stressed. To predict performance of hybrids on the basis of their inbred lines’, secondary traits of inbred lines were investigated to establish their relationships with grain yield of their respective hybrids under optimum and Low nitrogen levels. Therelationships between hybrid grain yield and yield secondary traits of inbred lines under optimum and Low nitrogen levelswere determined. Yield secondary traits studied included Ears per plant (EPP), Chlorophyll content (CC), Plant height (PH) and AnthesisSilking Interval (ASI).Thirty seven (37) Hybrids, their parents and three local checks (MRI 624, MRI 514 and ZMS 606) were evaluated in a randomised complete block design (RCBD) under optimum and low N conditions at Golden Valley Agricultural Research Trust (GART) Chisamba farm.Under optimum N plant height and Chlorophyll content significantly (45% and 26%, respectively) affected grain yield of inbred lines.For hybrids, plant height and ears per plant had significant influence of 11.4% and 37%, respectively on GY under low N conditions. Also, Plant height had significant influence (35%) on GY under optimum conditions. Plant height and Chlorophyll content of inbred lines had a significant influence of 28 % and 7% respectively on hybrid grain yield. Plant height of inbred lines had a significant influence of 28 % on hybrid grain yield. Indeed,Chlorophyll content showed a significant influence of 7 % of the total variation in Hybrid grain yield. Conclusions were that low N depresses performance, in terms of yield, of both inbred lines and hybrids. The reduction in yield is associated with a reduction in EPP, PH and CC and an increase in ASI. Plant height influenced grain yield of the inbred lines under low N stress conditions. Under optimum conditions it was plant height and CCthat positively influenced grain yield. For hybrids under low N conditions, plant height and ears per plant positively influenced grain yield of the hybrids, while under optimum conditions, only plant height positively influenced grain yield. Under low N conditions, plant height and CCof inbred lines positively influenced hybrid grain yield. Thus,if inbred lines with increased chlorophyll content and plant height under low N are selected, they would give Hybrids with higher yields under small holder farmer conditions which are generally low N conditions in Zambia.

Subjects/Keywords: Plant Breeding; Plant genetic Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Musonda, K. L. (2014). Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen . (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/3548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Musonda, Kanyanta LLoyd. “Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen .” 2014. Thesis, University of Zambia. Accessed January 20, 2021. http://hdl.handle.net/123456789/3548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Musonda, Kanyanta LLoyd. “Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen .” 2014. Web. 20 Jan 2021.

Vancouver:

Musonda KL. Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen . [Internet] [Thesis]. University of Zambia; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/123456789/3548.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Musonda KL. Use of inbred line Secondary traits in predicting performance in Maize(Zea Mays L.)Hybrid under low Nitrogen . [Thesis]. University of Zambia; 2014. Available from: http://hdl.handle.net/123456789/3548

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

10. Balaji A N. Genetic and simulated annealing algorithms for fixed charge transportation models;.

Degree: Genetic and simulated annealing algorithms for fixed charge transportation models, 2015, Anna University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/34351

►

The fixed charge transportation FCT problem is an extension of newlinethe classical transportation problem in which two kinds of cost are involved a newlinecontinuous cost… (more)

Subjects/Keywords:

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

N, B. A. (2015). Genetic and simulated annealing algorithms for fixed charge transportation models;. (Thesis). Anna University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/34351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

N, Balaji A. “Genetic and simulated annealing algorithms for fixed charge transportation models;.” 2015. Thesis, Anna University. Accessed January 20, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/34351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

N, Balaji A. “Genetic and simulated annealing algorithms for fixed charge transportation models;.” 2015. Web. 20 Jan 2021.

Vancouver:

N BA. Genetic and simulated annealing algorithms for fixed charge transportation models;. [Internet] [Thesis]. Anna University; 2015. [cited 2021 Jan 20]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/34351.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

N BA. Genetic and simulated annealing algorithms for fixed charge transportation models;. [Thesis]. Anna University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/34351

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Johannesburg

11. Mkhonza, Nompumelelo Lucritia. Development of in vitro and transformation methods for Sorghum bicolor (L. Moench).

Degree: 2010, University of Johannesburg

URL: http://hdl.handle.net/10210/3496

►

M.Tech.

Sorghum [Sorghum bicolor (L.)] is classified as a relatively recalcitrant crop due to its poor amenability to in vitro and genetic manipulation. An efficient… (more)

Subjects/Keywords: Sorghum micropropagation; Sorghum genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mkhonza, N. L. (2010). Development of in vitro and transformation methods for Sorghum bicolor (L. Moench). (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/3496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mkhonza, Nompumelelo Lucritia. “Development of in vitro and transformation methods for Sorghum bicolor (L. Moench).” 2010. Thesis, University of Johannesburg. Accessed January 20, 2021. http://hdl.handle.net/10210/3496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mkhonza, Nompumelelo Lucritia. “Development of in vitro and transformation methods for Sorghum bicolor (L. Moench).” 2010. Web. 20 Jan 2021.

Vancouver:

Mkhonza NL. Development of in vitro and transformation methods for Sorghum bicolor (L. Moench). [Internet] [Thesis]. University of Johannesburg; 2010. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10210/3496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mkhonza NL. Development of in vitro and transformation methods for Sorghum bicolor (L. Moench). [Thesis]. University of Johannesburg; 2010. Available from: http://hdl.handle.net/10210/3496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Aboluion, Niema Ali. The construction of DNA codes using a computer algebra system.

Degree: PhD, University of Glamorgan, 2012, University of South Wales

URL: http://hdl.handle.net/10265/467

► Coding theory has several applications in Genetics and Bioengineering. This thesis concentrates on a specific application from Computational Biology. This concerns the construction of new… (more)

▼ Coding theory has several applications in Genetics and Bioengineering. This thesis concentrates on a specific application from Computational Biology. This concerns the construction of new DNA codes which satisfy certain combinatorial constraints, using an alphabet of four symbols. The interest in these codes arises because it is possible to synthesise short single strands of DNA known as oligonucleotides. The codes can be useful in the design of these oligonucleotides. For example, the codes are used in DNA computing, as bar codes in molecular libraries and in microarray technologies. The computer algebra system Magma, which deals successfully with coding theory computation, is applied initially to the construction of DNA codes sat- isfying a GC-content constraint and a minimum Hamming distance constraint. The constraints are specified to avoid unwanted hybridizations and to ensure uniform melting temperatures. Additionally, another constraint, known as a reverse-complement constraint, is added to further prevent unwanted hybridiza- tions. This additional constraint is studied using involutions in a permutation group. Codes constructed in this thesis are derived from linear codes over GF(4) and Z4 and additive codes over GF(4). Previous approaches to the construction of these codes are extended in several ways. Longer codes are constructed, the examination of cyclic and extended cyclic codes is more comprehensive, and cosets of codes are considered. In addition, attention is paid to the mapping from field or ring elements to the DNA nucleotides; different mappings can give different lower bounds. Further improvements have been made after the tech- niques of shortening and puncturing are applied to the table of best codes, and also by searching for codes in the tables that have an all-ones vector in their dual. The use of a database of best known linear codes is also considered. In many cases codes are obtained which are larger than the best codes currently known. In the case of codes of length greater than twenty, linear DNA codes have not been constructed previously and so all codes obtained are the best known re- sults. Generator polynomials are given for the codes constructed. Coset leaders are also given in cases where cosets of linear codes are used. Thus it is possible for the reader to construct the codes without repeating the work presented in the thesis. Additionally, files of codewords are available online when the codes constructed are the best known codes and have fewer than 50000 codewords.

Subjects/Keywords: Genetic engineering; Biological systems; 572.86

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboluion, N. A. (2012). The construction of DNA codes using a computer algebra system. (Doctoral Dissertation). University of South Wales. Retrieved from http://hdl.handle.net/10265/467

Chicago Manual of Style (16^th Edition):

Aboluion, Niema Ali. “The construction of DNA codes using a computer algebra system.” 2012. Doctoral Dissertation, University of South Wales. Accessed January 20, 2021. http://hdl.handle.net/10265/467.

MLA Handbook (7^th Edition):

Aboluion, Niema Ali. “The construction of DNA codes using a computer algebra system.” 2012. Web. 20 Jan 2021.

Vancouver:

Aboluion NA. The construction of DNA codes using a computer algebra system. [Internet] [Doctoral dissertation]. University of South Wales; 2012. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10265/467.

Council of Science Editors:

Aboluion NA. The construction of DNA codes using a computer algebra system. [Doctoral Dissertation]. University of South Wales; 2012. Available from: http://hdl.handle.net/10265/467

13. Dumisic, Sanjin. The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century.

Degree: philosophical and religious studies, 2016, Umeå University

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-123167

► This essay discusses, departing from Kant’s deontological ethics, and deals with the ques- tion of which types of pre-birth genetic modification are acceptable from… (more)

Subjects/Keywords: Kant; Transhumanism; Bioethics; Genetic Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dumisic, S. (2016). The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century. (Thesis). Umeå University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-123167

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dumisic, Sanjin. “The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century.” 2016. Thesis, Umeå University. Accessed January 20, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-123167.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dumisic, Sanjin. “The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century.” 2016. Web. 20 Jan 2021.

Vancouver:

Dumisic S. The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century. [Internet] [Thesis]. Umeå University; 2016. [cited 2021 Jan 20]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-123167.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dumisic S. The Choice of Pre-Birth Genetic Modification : Through Kant´s Ethics in the 21st Century. [Thesis]. Umeå University; 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-123167

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Aboluion, Niema Ali. The construction of DNA codes using a computer algebra system.

Degree: PhD, 2011, University of South Wales

URL: https://pure.southwales.ac.uk/en/studentthesis/the-construction-of-dna-codes-using-a-computer-algebra-system(d0ee33ce-c640-407d-868c-ba5eafa81909).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534415

► Coding theory has several applications in Genetics and Bioengineering. This thesis concentrates on a specific application from Computational Biology. This concerns the construction of new… (more)

▼ Coding theory has several applications in Genetics and Bioengineering. This thesis concentrates on a specific application from Computational Biology. This concerns the construction of new DNA codes which satisfy certain combinatorial constraints, using an alphabet of four symbols. The interest in these codes arises because it is possible to synthesise short single strands of DNA known as oligonucleotides. The codes can be useful in the design of these oligonucleotides. For example, the codes are used in DNA computing, as bar codes in molecular libraries and in microarray technologies. The computer algebra system Magma, which deals successfully with coding theory computation, is applied initially to the construction of DNA codes sat- isfying a GC-content constraint and a minimum Hamming distance constraint. The constraints are specified to avoid unwanted hybridizations and to ensure uniform melting temperatures. Additionally, another constraint, known as a reverse-complement constraint, is added to further prevent unwanted hybridiza- tions. This additional constraint is studied using involutions in a permutation group. Codes constructed in this thesis are derived from linear codes over GF(4) and Z4 and additive codes over GF(4). Previous approaches to the construction of these codes are extended in several ways. Longer codes are constructed, the examination of cyclic and extended cyclic codes is more comprehensive, and cosets of codes are considered. In addition, attention is paid to the mapping from field or ring elements to the DNA nucleotides; different mappings can give different lower bounds. Further improvements have been made after the tech- niques of shortening and puncturing are applied to the table of best codes, and also by searching for codes in the tables that have an all-ones vector in their dual. The use of a database of best known linear codes is also considered. In many cases codes are obtained which are larger than the best codes currently known. In the case of codes of length greater than twenty, linear DNA codes have not been constructed previously and so all codes obtained are the best known re- sults. Generator polynomials are given for the codes constructed. Coset leaders are also given in cases where cosets of linear codes are used. Thus it is possible for the reader to construct the codes without repeating the work presented in the thesis. Additionally, files of codewords are available online when the codes constructed are the best known codes and have fewer than 50000 codewords.

Subjects/Keywords: 572.86; Genetic engineering; Biological systems

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboluion, N. A. (2011). The construction of DNA codes using a computer algebra system. (Doctoral Dissertation). University of South Wales. Retrieved from https://pure.southwales.ac.uk/en/studentthesis/the-construction-of-dna-codes-using-a-computer-algebra-system(d0ee33ce-c640-407d-868c-ba5eafa81909).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534415

Chicago Manual of Style (16^th Edition):

Aboluion, Niema Ali. “The construction of DNA codes using a computer algebra system.” 2011. Doctoral Dissertation, University of South Wales. Accessed January 20, 2021. https://pure.southwales.ac.uk/en/studentthesis/the-construction-of-dna-codes-using-a-computer-algebra-system(d0ee33ce-c640-407d-868c-ba5eafa81909).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534415.

MLA Handbook (7^th Edition):

Aboluion, Niema Ali. “The construction of DNA codes using a computer algebra system.” 2011. Web. 20 Jan 2021.

Vancouver:

Aboluion NA. The construction of DNA codes using a computer algebra system. [Internet] [Doctoral dissertation]. University of South Wales; 2011. [cited 2021 Jan 20]. Available from: https://pure.southwales.ac.uk/en/studentthesis/the-construction-of-dna-codes-using-a-computer-algebra-system(d0ee33ce-c640-407d-868c-ba5eafa81909).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534415.

Council of Science Editors:

Aboluion NA. The construction of DNA codes using a computer algebra system. [Doctoral Dissertation]. University of South Wales; 2011. Available from: https://pure.southwales.ac.uk/en/studentthesis/the-construction-of-dna-codes-using-a-computer-algebra-system(d0ee33ce-c640-407d-868c-ba5eafa81909).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534415

University of Oxford

15. Ababneh, Nidaa. Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC).

Degree: PhD, 2017, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:b0e48523-2acc-4c1e-83a5-79696cbaf042 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729403

► The hexanucleotide repeat expansion (HRE) mutation within C9orf72 gene is the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Several hypotheses… (more)

Subjects/Keywords: 616.8; Neurosciences and genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ababneh, N. (2017). Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC). (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:b0e48523-2acc-4c1e-83a5-79696cbaf042 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729403

Chicago Manual of Style (16^th Edition):

Ababneh, Nidaa. “Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC).” 2017. Doctoral Dissertation, University of Oxford. Accessed January 20, 2021. http://ora.ox.ac.uk/objects/uuid:b0e48523-2acc-4c1e-83a5-79696cbaf042 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729403.

MLA Handbook (7^th Edition):

Ababneh, Nidaa. “Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC).” 2017. Web. 20 Jan 2021.

Vancouver:

Ababneh N. Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC). [Internet] [Doctoral dissertation]. University of Oxford; 2017. [cited 2021 Jan 20]. Available from: http://ora.ox.ac.uk/objects/uuid:b0e48523-2acc-4c1e-83a5-79696cbaf042 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729403.

Council of Science Editors:

Ababneh N. Modelling of amyotrophic lateral sclerosis (ALS) using induced pluripotent stem cells (iPSC). [Doctoral Dissertation]. University of Oxford; 2017. Available from: http://ora.ox.ac.uk/objects/uuid:b0e48523-2acc-4c1e-83a5-79696cbaf042 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.729403

University of Missouri – Columbia

16. Joshi, Trupti, 1977-. Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max.

Degree: 2013, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/43323

► [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Many genome-scale data are available in soybean (Glycine Max) including genomics, transcriptomics, proteomics and metabolomics… (more)

Subjects/Keywords: Soybean – Genetic engineering – Databases.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Joshi, Trupti, 1. (2013). Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/43323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Joshi, Trupti, 1977-. “Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max.” 2013. Thesis, University of Missouri – Columbia. Accessed January 20, 2021. http://hdl.handle.net/10355/43323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Joshi, Trupti, 1977-. “Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max.” 2013. Web. 20 Jan 2021.

Vancouver:

Joshi, Trupti 1. Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max. [Internet] [Thesis]. University of Missouri – Columbia; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10355/43323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Joshi, Trupti 1. Development of Soybean Knowledge Base (SoyKB), a multi-omics data integration web resource for bridging molecular breeding and translational genomics in Glycine Max. [Thesis]. University of Missouri – Columbia; 2013. Available from: http://hdl.handle.net/10355/43323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hawaii – Manoa

17. Singh, J. Malkeet. Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology.

Degree: MS, 2011, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/20738

►

viii, 56 leaves, bound ill. (some col.) 29 cm

The main goal of this thesis is to show that functional genomics studies can be conducted… (more)

Subjects/Keywords: Nicotiana benthamiana – Genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Singh, J. M. (2011). Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology. (Masters Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/20738

Chicago Manual of Style (16^th Edition):

Singh, J Malkeet. “Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology.” 2011. Masters Thesis, University of Hawaii – Manoa. Accessed January 20, 2021. http://hdl.handle.net/10125/20738.

MLA Handbook (7^th Edition):

Singh, J Malkeet. “Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology.” 2011. Web. 20 Jan 2021.

Vancouver:

Singh JM. Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology. [Internet] [Masters thesis]. University of Hawaii – Manoa; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10125/20738.

Council of Science Editors:

Singh JM. Isolation and characterization of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and putrescine N-methyl transferase (PMT) complementary deoxyribonucleic acid (cDNA) in Nicotiana benthamiana using cytoplasmic inhibition of gene expression (CIGE) technology. [Masters Thesis]. University of Hawaii – Manoa; 2011. Available from: http://hdl.handle.net/10125/20738

University of New South Wales

18. Zhao, Xun. Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products.

Degree: Biotechnology & Biomolecular Sciences, 2011, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/50943 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9837/SOURCE02?view=true

► Zymomonas mobilis has attracted considerable interest over the past decades as a result of its unique metabolism and ability to rapidly andefficiently produce ethanol. Flocculation… (more)

▼ Zymomonas mobilis has attracted considerable interest over the past decades as a result of its unique metabolism and ability to rapidly andefficiently produce ethanol. Flocculation in Z. mobilis is important commercially as a cost effective means of microbial biomass recycling tofacilitate ethanol recovery and increase volumetric productivity. In this study, a flocculant mutant Z. mobilis strain ZM401 (derived from wild typestrain ZM4) was used to elucidate the molecular basis of flocculation in Z. mobilis. The results of microarray analysis revealed that approximately30 genes were significantly differentially expressed. Functional annotation indicated that the major group of genes up-regulated belonged to acellulose synthase group (BcsA, BcsB, BcsC), while the major genes down-regulated were a group of flagella synthesis proteins (FlgBCDEF,FlgH, FliG). A further experiment established that the enzyme cellulase could efficiently deflocculate cell aggregates of ZM401, which indicatedthat cellulose biosynthesis was a major mechanism of cell flocculation in Z. mobilis.Studies on the inactivation of pyruvate decarboxylase (pdc) in Z. mobilis were carried out and to evaluate the possibility of a carbon-flux shift fromthe Entner-Doudoroff pathway towards higher value fermentation products such as (succinate, lactate). The enzymatic assay for PDC activity fromthe pdc inactivated strain ZM4812 confirmed that a 50% reduction in enzyme activity had occured. Fermentation experiments confirmed thereduced expression level of PDC activity resulted in reduced rates of substrate uptake and ethanol production, together with increased pyruvateaccumulation and trace amounts of lactate and succinate. The increased pyruvate and trace amounts of lactate and succinate suggested thatother enzymes such as pyruvate dehydrogenase (PDH) and lactate dehydrogenase and possibly other enzymes involved in production of succinicacid could serve as metabolic bottlenecks for their end-product production. Preliminary experiments were also carried out for increased PDHactivity in Z. mobilis.

Subjects/Keywords: Zymomonas mobilis; Genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, X. (2011). Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/50943 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9837/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Zhao, Xun. “Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products.” 2011. Masters Thesis, University of New South Wales. Accessed January 20, 2021. http://handle.unsw.edu.au/1959.4/50943 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9837/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Zhao, Xun. “Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products.” 2011. Web. 20 Jan 2021.

Vancouver:

Zhao X. Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products. [Internet] [Masters thesis]. University of New South Wales; 2011. [cited 2021 Jan 20]. Available from: http://handle.unsw.edu.au/1959.4/50943 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9837/SOURCE02?view=true.

Council of Science Editors:

Zhao X. Genetic characterization and manipulation of strains of Zymomonas mobilis for ethanol and higher value products. [Masters Thesis]. University of New South Wales; 2011. Available from: http://handle.unsw.edu.au/1959.4/50943 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:9837/SOURCE02?view=true

Michigan State University

19. Nguyen, Thang Xuan. Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance.

Degree: 2012, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:1566

►

Thesis Ph. D. Michigan State University. Crop and Soil Sciences 2012.

MULTIPLE TRANSGENE ENGINEERING FOR MAIZE (Zea mays L.) DROUGHT AND SALT TOLERANCEByThang Xuan Nguyen… (more)

▼

Thesis Ph. D. Michigan State University. Crop and Soil Sciences 2012.

MULTIPLE TRANSGENE ENGINEERING FOR MAIZE (Zea mays L.) DROUGHT AND SALT TOLERANCEByThang Xuan Nguyen Maize (Zea mays L.) is an important basic food and feed grain, and its stover is used for animal feed and biofuel. Increased soil-salinity and water-deficiency are the two major factors limiting the maize plant growth and development and subsequently affecting its grain and biomass yields, and the yield. The research in this dissertation is focused on multiple transgene engineering (transgene pyramiding) for drought and salt tolerance in maize. In the research presented in this dissertation, genetic transformation of maize was performed via the gene gun bombardment of embryogenic immature embryos of maize using single and multiple constructs, pBY520 containing the barley (Hordeum vulgare) HVA1 and the JS101 containing the bacterial mannitol 1 phosphate dehydrogenase (mtlD), both genes regulated by rice actin promoter and potato protease inhibitor II terminator. There were two gene linked cassettes in each of these two constructs, one cassette containing the abiotic stress tolerance gene of interest (HVA1 or mtlD) and the other containing the bar herbicide resistance gene regulated by cauliflower mosaic virus 35S promoter and nos terminator. The pBY520 and JS101 constructs were co-bombarded in 1:1 ratio into maize genome for transgene pyramiding. The confirmation of transgene integration and expression were made via molecular techniques, including polymerase chain reaction (PCR) for transgene integration, and reverse transcriptase (RT) PCR and Northern blotting for transgene transcription. Southern blotting was performed to find the number of copies of each transgene in transgenic plants. Results showed stable integration and expression of the HVA1, mtlD and HVA1-mtlD in transgenic maize plants. Up to 4th generation transgenic (T3) plants were produced, with all progenies showing the co-integration of abiotic stress tolerance genes of interests and the bar gene with a frequency of 100%. The single HVA1 or mtlD transgenic plants showed higher leaf relative water content (RWC) and higher percent of plant survival as compared to their wild-type non-transgenic control plant counterparts under water withholding condition for 15 days followed by 7 days of re-watering. When exposed to different salt concentrations (0, 100, 200 and 300 mM NaCl) for 10 days, the HVA1 and mtlD transgenic plants showed higher fresh and dry shoot and dry root biomass matter as compared to their wild-type non-transgenic control plants. The research also demonstrated that the mtlD transgenic plants that were salt tolerant, also accumulated mannitol in their cells. More research is needed on mannitol accumulation in transgenic plants to see whether the mannitol level measured by gas chromatography (GC) was not partially or totally representing sorbitol accumulation. Considering that mannitol and sorbitol are both osmoprotectants, the salt tolerance of the mtlD transgenic plants…

Advisors/Committee Members: Sticklen, Mariam, Freed, Russell, Wang, Dechun, Hancock, James.

Subjects/Keywords: Transgenes; Genetic engineering; Corn – Genetic engineering; Salt-tolerant crops – Genetic engineering; Corn – Drought tolerance – Genetic aspects; Agriculture

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nguyen, T. X. (2012). Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:1566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nguyen, Thang Xuan. “Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance.” 2012. Thesis, Michigan State University. Accessed January 20, 2021. http://etd.lib.msu.edu/islandora/object/etd:1566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nguyen, Thang Xuan. “Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance.” 2012. Web. 20 Jan 2021.

Vancouver:

Nguyen TX. Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance. [Internet] [Thesis]. Michigan State University; 2012. [cited 2021 Jan 20]. Available from: http://etd.lib.msu.edu/islandora/object/etd:1566.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen TX. Multiple transgene engineering for maize (Zea mays L.) drought and salt tolerance. [Thesis]. Michigan State University; 2012. Available from: http://etd.lib.msu.edu/islandora/object/etd:1566

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

20. Temme, Karsten Louis. Designing and Engineering Complex Behavior in Living Machines.

Degree: Bioengineering, 2011, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/1r41x99s

► Living organisms exhibit many fascinating behaviors that profoundly impact the world surrounding us. Some of these functions have been harnessed within biotechnology and redirected towards… (more)

Subjects/Keywords: Biomedical engineering; Systematic biology; Microbiology; genetic circuits; genetic engineering; synthetic biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Temme, K. L. (2011). Designing and Engineering Complex Behavior in Living Machines. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/1r41x99s

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Temme, Karsten Louis. “Designing and Engineering Complex Behavior in Living Machines.” 2011. Thesis, University of California – Berkeley. Accessed January 20, 2021. http://www.escholarship.org/uc/item/1r41x99s.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Temme, Karsten Louis. “Designing and Engineering Complex Behavior in Living Machines.” 2011. Web. 20 Jan 2021.

Vancouver:

Temme KL. Designing and Engineering Complex Behavior in Living Machines. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2021 Jan 20]. Available from: http://www.escholarship.org/uc/item/1r41x99s.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Temme KL. Designing and Engineering Complex Behavior in Living Machines. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/1r41x99s

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Yuan, Dawei. Biotechnological interventions for crop improvement in the context of food security.

Degree: Departament de Producció Vegetal i Ciència Forestal, 2012, Universitat de Lleida

URL: http://hdl.handle.net/10803/84103

► Crop productivity is limited by a number of important constraints that need to be addressed urgently in order to avoid an imminent humanitarian crisis. My… (more)

Subjects/Keywords: Biotecnologia; Seguritat alimentaria; Cultius; Millora; Enginyeria genètica; Biotecnologia; Seguridad alimentaria; Cultivos; Mejorados; ingenieria genética; Biotechnological; Food security; Crops; Improvement; Genetic engineering; Bioquímica i Biologia Molecular; 577

…engineering in plants will teach us not only how to engineer biochemical changes but also about how… …approaching the natural ceiling, so the possibility of genetic intervention to increase…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yuan, D. (2012). Biotechnological interventions for crop improvement in the context of food security. (Thesis). Universitat de Lleida. Retrieved from http://hdl.handle.net/10803/84103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yuan, Dawei. “Biotechnological interventions for crop improvement in the context of food security.” 2012. Thesis, Universitat de Lleida. Accessed January 20, 2021. http://hdl.handle.net/10803/84103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yuan, Dawei. “Biotechnological interventions for crop improvement in the context of food security.” 2012. Web. 20 Jan 2021.

Vancouver:

Yuan D. Biotechnological interventions for crop improvement in the context of food security. [Internet] [Thesis]. Universitat de Lleida; 2012. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10803/84103.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yuan D. Biotechnological interventions for crop improvement in the context of food security. [Thesis]. Universitat de Lleida; 2012. Available from: http://hdl.handle.net/10803/84103

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

22. Mosabbir, Abdullah Al. Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling.

Degree: PhD, 2019, University of Toronto

URL: http://hdl.handle.net/1807/95931

► A major challenge of cell-based therapies is the appropriate and effective localization of cells to target sites. Engineering cell migration to a specific target site… (more)

▼ A major challenge of cell-based therapies is the appropriate and effective localization of cells to target sites. Engineering cell migration to a specific target site can offer a promising solution to this problem. Previously, a chimeric protein known as CaRQ was developed to modulate cell migration based on intracellular Ca2+ transients. CaRQ confers versatility in rewiring cell migration to chemical and physical stimuli, by combining CaRQ with receptors that generate Ca2+ transients. Through in vitro cell-based experiments, this thesis demonstrates the versatility of CaRQ in engineering cell migration in response to chemical (i.e. cytokines) and physical (i.e. magnetic fields) stimuli. First, migration towards vascular endothelial growth factor (VEGF) was engineered by a system composed of CaRQ and the VEGF receptor 2 (VEGFR2). VEGF binds to VEGFR2 and generates an intracellular Ca2+ transient. Stable cells expressing VEGFR2 and CaRQ displayed migratory blebbing, migration across porous membranes, directed migration, as well as wound healing in response to VEGF and VEGFR2 antibodies. Second, migration towards granulocyte-macrophage colony-stimulating factor (GM-CSF) was engineered by a system composed of CaRQ and a chimeric receptor (henceforth GMRchi). GMRchi is composed of the VEGFR2 cytoplasmic domain and GM-CSF receptor extracellular domain. Since the GM-CSF receptor alone cannot generate Ca2+transients, the chimeric receptor was engineered, demonstrating that migration is not limited to the repertoire of naturally occurring receptors. Stable cells expressing GMRchi and CaRQ displayed migratory blebbing, migration across porous membranes, directed migration, as well as wound healing in response to GM-CSF. Third, cell migration towards physical stimuli (specifically magnetic fields) was engineered by a system composed of CaRQ and a magnetic-sensitive gene circuit (henceforth the TF circuit). The TF circuit is composed of a chimeric TRPV1 receptor and ferritin. It was observed that a magnetic field induced migration when the TF circuit was paired with CaRQ. Taken together, this thesis demonstrates the utility of CaRQ in engineering cell migration to different stimuli in vitro, thus taking a step forward in engineering a versatile system to localize cells for cell-based therapy. It remains to be seen if these genetically-encoded systems will have efficacy in vivo. Advisors/Committee Members: Truong, Kevin, Biomedical Engineering.

Subjects/Keywords: Genetic Engineering; Protein Engineering; Synthetic Biology; 0379

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mosabbir, A. A. (2019). Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/95931

Chicago Manual of Style (16^th Edition):

Mosabbir, Abdullah Al. “Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling.” 2019. Doctoral Dissertation, University of Toronto. Accessed January 20, 2021. http://hdl.handle.net/1807/95931.

MLA Handbook (7^th Edition):

Mosabbir, Abdullah Al. “Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling.” 2019. Web. 20 Jan 2021.

Vancouver:

Mosabbir AA. Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling. [Internet] [Doctoral dissertation]. University of Toronto; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1807/95931.

Council of Science Editors:

Mosabbir AA. Engineering Cell Migration to Chemical and Physical Stimuli using Ca2+ Based Signalling. [Doctoral Dissertation]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/95931

Rutgers University

23. Yu, Qiguo, 1989-. Novel tools for plastid engineering in higher plants.

Degree: PhD, Plant Biology, 2020, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/63085/

►

Plastid genome engineering holds great promise in many biotechnological applications, including production of vaccines and antibodies, conferring insect tolerance by expressing dsRNAs, implanting novel metabolic… (more)

▼

Plastid genome engineering holds great promise in many biotechnological applications, including production of vaccines and antibodies, conferring insect tolerance by expressing dsRNAs, implanting novel metabolic pathways and improving photosynthetic efficiency and so on. However, the plastid transformation technology is not yet available in most crop plants. I was part of the group that developed a system for plastid transformation in Arabidopsis thaliana, a Brassicaceae species, that is potentially applicable to the important oilseed crop canola (Brassica napus) and vegetable brassicas such as broccoli, cauliflower and cabbage (Brassica oleracea). Efficient plastid transformation is enabled by eliminating a duplicate fatty acid biosynthetic pathway in chloroplasts. High level expression of recombinant proteins is desirable, but it may cripple the plant growth. To achieve on demand expression of plastid transgenes, I was involved in a project that exploited the use of an engineered PPR (pentatricopeptide) RNA-binding protein for post-transcriptional regulation of chloroplast genes. One application is ethanol-inducible expression of chloroplast proteins by activating mRNA translation when the protein is needed. Thus, the transplastomic plants can be grown full size in the absence of the inducer and expression is turned on during the protein production phase. The second application is tuber-specific expression of plastid genes where plastid gene expression is normally very law. When the engineered PPR variant is expressed from a tuber-specific patatin promoter, a 60-fold increase over 0.02%, the maximum protein yield achieved to date, has been obtained in the potato tuber. Plastids represent an appealing target for synthetic biology applications, where predictable protein output is of paramount importance. I designed synthetic operons from which reporter gene expression could be obtained in tobacco chloroplasts over a large dynamic range by post-transcriptional regulation, using the right combination of RNA binding proteins, RNA-binding protein binding sites and RNA processing elements.

Advisors/Committee Members: Maliga, Pal (chair), Dong, Juan (internal member), Gallavotti, Andrea (internal member), Nickels, Bryce (outside member), School of Graduate Studies.

Subjects/Keywords: Chloroplast engineering higher plants; Plastids – Genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, Qiguo, 1. (2020). Novel tools for plastid engineering in higher plants. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/63085/

Chicago Manual of Style (16^th Edition):

Yu, Qiguo, 1989-. “Novel tools for plastid engineering in higher plants.” 2020. Doctoral Dissertation, Rutgers University. Accessed January 20, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/63085/.

MLA Handbook (7^th Edition):

Yu, Qiguo, 1989-. “Novel tools for plastid engineering in higher plants.” 2020. Web. 20 Jan 2021.

Vancouver:

Yu, Qiguo 1. Novel tools for plastid engineering in higher plants. [Internet] [Doctoral dissertation]. Rutgers University; 2020. [cited 2021 Jan 20]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/63085/.

Council of Science Editors:

Yu, Qiguo 1. Novel tools for plastid engineering in higher plants. [Doctoral Dissertation]. Rutgers University; 2020. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/63085/

24. Matsaunyane, Lerato Bame Tsalaemang. The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects.

Degree: 2014, University of Johannesburg

URL: http://hdl.handle.net/10210/12350

►

Ph.D. (Biochemistry)

Genetically modified (GM) crops were first introduced in the 1980s for the production of medicinal products. Since then, areas designated to GM crops… (more)

▼

Ph.D. (Biochemistry)

Genetically modified (GM) crops were first introduced in the 1980s for the production of medicinal products. Since then, areas designated to GM crops have expanded drastically, with the GM crops grown to enhance agricultural productivity, improve agricultural practices, and as a tool to address potential pressures that will be faced by the agricultural sector and to address the issue of food security. Currently, cultivated GM crops include cotton, maize, rapeseed and soybean, carrying agronomic traits such as herbicide tolerance and insect resistance. Following the genetic modification of crops, three possible outcomes can be anticipated: these outcomes include the GM crop produced being equivalent to its untransformed counterpart, the GM crop differing from its untransformed counterpart with several well-defined characteristics, and the GM crop differing from its untransformed counterpart with a multitude of complex characteristics. In cases where the GM crop is equivalent to the untransformed counterpart, no further testing is needed. In instances where several well-defined and characterised differences are found between the GM crop and the untransformed counterpart, safety assessments are performed targeting these differences. The assessments will determine the impact of these unintended and unexpected alterations of the intended enhancement of the GM crops. However, methods currently used to assess GM crops have been found to be lacking, since they only focus on environmental and product-specific risks. Further evidence is essential, as part of GM crop safety assessment, on the molecular characterisation of these crops. This evidence is based on the potential impact of the transformation event, integration of the transgene into the host plant, as well as unintended alterations such as altered gene expression that may occur to the host plant. These events may assist in the further detection of potential dangers of the GM crop. As a result of these highlighted gaps, a project was formulated to study the unintended genomic alterations that may occur during and following the production of a transgenic plant...

Subjects/Keywords: Plant genetic transformation; Potatoes - Genetic engineering; Wilt diseases - Prevention; Glycoproteins - Synthesis

12 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matsaunyane, L. B. T. (2014). The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/12350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matsaunyane, Lerato Bame Tsalaemang. “The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects.” 2014. Thesis, University of Johannesburg. Accessed January 20, 2021. http://hdl.handle.net/10210/12350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matsaunyane, Lerato Bame Tsalaemang. “The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects.” 2014. Web. 20 Jan 2021.

Vancouver:

Matsaunyane LBT. The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects. [Internet] [Thesis]. University of Johannesburg; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10210/12350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matsaunyane LBT. The transformation of Solanum tuberosum with the PGIP1 gene from Malus domestica : molecular analysis of the gene insertion event and screening for unintended effects. [Thesis]. University of Johannesburg; 2014. Available from: http://hdl.handle.net/10210/12350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Harvard University

25. Lajoie, Marc Joseph. Genome Engineering Technologies to Change the Genetic Code.

Degree: PhD, Chemical Biology, 2014, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697

► New technologies are making it possible to engineer organisms with fundamentally new and useful properties. In vivo genome engineering technologies capable of manipulating genomes from… (more)

Subjects/Keywords: Biology; Genetic code; Genetic isolation; Genome engineering; MAGE; Recombination; Virus resistance

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lajoie, M. J. (2014). Genome Engineering Technologies to Change the Genetic Code. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697

Chicago Manual of Style (16^th Edition):

Lajoie, Marc Joseph. “Genome Engineering Technologies to Change the Genetic Code.” 2014. Doctoral Dissertation, Harvard University. Accessed January 20, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697.

MLA Handbook (7^th Edition):

Lajoie, Marc Joseph. “Genome Engineering Technologies to Change the Genetic Code.” 2014. Web. 20 Jan 2021.

Vancouver:

Lajoie MJ. Genome Engineering Technologies to Change the Genetic Code. [Internet] [Doctoral dissertation]. Harvard University; 2014. [cited 2021 Jan 20]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697.

Council of Science Editors:

Lajoie MJ. Genome Engineering Technologies to Change the Genetic Code. [Doctoral Dissertation]. Harvard University; 2014. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697

Columbia University

26. Johns, Nathan Isaac. Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution.

Degree: 2019, Columbia University

URL: https://doi.org/10.7916/d8-8xw1-7d69

► Mechanistic understanding of gene regulation is crucial for rational engineering of new genetic systems through synthetic biology. Genetic engineering efforts in new organisms are often… (more)

▼ Mechanistic understanding of gene regulation is crucial for rational engineering of new genetic systems through synthetic biology. Genetic engineering efforts in new organisms are often hampered by a lack of knowledge about how regulatory components function in new host contexts. This dissertation focuses on efforts to overcome these challenges through the development of generalizable experimental methods for studying the behavior of DNA regulatory sequences in diverse species at large-scale. Chapter 2 describes experimental approaches for quantitatively assessing the functions of thousands of diverse natural regulatory sequences through a combination of metagenomic mining, high-throughput DNA synthesis and deep sequencing. By employing these methods in three distinct bacterial species, we revealed striking functional differences in gene regulatory capacity. We identified regulatory sequences with activity levels with activity levels spanning several orders of magnitude, which will aid in efforts to engineer diverse bacterial species. We also demonstrate functional species-selective gene circuits with programmable host behaviors that may be useful for microbial community engineering. In Chapter 3 we provide evidence for the evolution of altered stringency in σ70-mediated transcriptional activation based on patterns of initiation and activity from promoters of diverse compositions. We show that the contrast in GC content between a regulatory element and the host genome dictates both the likelihood and the magnitude of expression. We also discuss the potential implications of this proposed mechanism on horizontal gene transfer. The next two chapters focus on efforts aimed at extending the high-throughput methods described in earlier chapters to new organisms. Chapter 4 presents an in vitro approach for multiplexed gene expression profiling. Through the development and use of cell-free expression systems made from diverse bacteria, it was possible to rapidly acquire thousands of transcriptional measurements in small volume reactions, enabling functional comparisons of regulatory sequence function across multiple species. In Chapter 5 we characterize the restriction-modification system repertoires of several commensal bacterial species. We also describe ongoing efforts to develop methods for bypassing these systems in order to increase transformation efficiencies in species that are difficult or impossible to transform using current approaches.

Subjects/Keywords: Microbiology; Genetics; Genetic regulation; Genetic engineering; Synthetic biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Johns, N. I. (2019). Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/d8-8xw1-7d69

Chicago Manual of Style (16^th Edition):

Johns, Nathan Isaac. “Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution.” 2019. Doctoral Dissertation, Columbia University. Accessed January 20, 2021. https://doi.org/10.7916/d8-8xw1-7d69.

MLA Handbook (7^th Edition):

Johns, Nathan Isaac. “Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution.” 2019. Web. 20 Jan 2021.

Vancouver:

Johns NI. Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution. [Internet] [Doctoral dissertation]. Columbia University; 2019. [cited 2021 Jan 20]. Available from: https://doi.org/10.7916/d8-8xw1-7d69.

Council of Science Editors:

Johns NI. Gene Regulatory Compatibility in Bacteria: Consequences for Synthetic Biology and Evolution. [Doctoral Dissertation]. Columbia University; 2019. Available from: https://doi.org/10.7916/d8-8xw1-7d69

IUPUI

27. K., S. INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES.

Degree: 2011, IUPUI

URL: http://hdl.handle.net/1805/2681

►

Indiana University-Purdue University Indianapolis (IUPUI)

There are many mechanisms for the creation of new genes. In this study, the newborn genes i.e. de novo genes… (more)

▼

Indiana University-Purdue University Indianapolis (IUPUI)

There are many mechanisms for the creation of new genes. In this study, the newborn genes i.e. de novo genes are the genes that are created from scratch. These are created by two mechanisms, polymerization (de novo genes produced from non-coding regions) and overprinting (de novo genes produced from overlapping frames). Rancurel et al has found that de novo genes in overlapping coding regions tend to be more disordered than their ancestral counterparts. It was suggested that it is natural for the newborn genes to be disordered, as it must be very difficult for newborn genes to obtain order at such an early stage, so that the structure is only developed after the evolutionary development. The two hypotheses tested in this study state (1) that genes generated de novo will have a tendency to be disordered, and (2) this tendency is due to a natural inclination of these genes to be disordered at birth. The origin and evolution of some de novo coding regions have been studied in detail. We analyzed genes reported in literature that have been produced de novo; either by overprinting or by polymerization, and their tendency for disorder was evaluated using the VSL2 disorder predictor. The de novo coding regions produced by both ways indeed shows a tendency towards disorder, which supports hypothesis 1. For hypothesis 2 to be tested on a larger dataset the exonic and intronic materials of two human chromosomes were studied and the tendency for disorder was assessed for any new peptide sequence arising from the translation of non-coding sequences arising from introns and exons (overlapping frames). It was shown that the tendency of disorder for protein products of newborn genes arising from introns were not inclined towards being ordered or disordered, but they can become disordered by evolution. The new exonic material created from the existing exons tends to be more disordered when translated, and this tendency does not seem to be dependent upon the disorder content of the original exons. This difference could be a consequence of the fact that the overlapping frames of coding sequences have indirectly been subjected to evolutionary pressure along with the original exon, whereas intronic sequences do not seem to have this constraint, but the exact nature of this discrepancy needs further study to be explained. The tendency of disorder in the existing new exons seems to be higher than the artificial exons (generated in this study). We conclude that the intrinsic disorder in the protein products of de novo genes is selected by the evolution rather than an initial condition. Thus, the newborn genes were not born disordered.

indefinitely

Advisors/Committee Members: Romero, Pedro, Perumal, Narayanan B., Dunker, Keith.

Subjects/Keywords: newborn gene; Genetic engineering; Genetic code; Nucleotide sequence

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

K., S. (2011). INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/2681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

K., S. “INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES.” 2011. Thesis, IUPUI. Accessed January 20, 2021. http://hdl.handle.net/1805/2681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

K., S. “INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES.” 2011. Web. 20 Jan 2021.

Vancouver:

K. S. INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES. [Internet] [Thesis]. IUPUI; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1805/2681.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

K. S. INTRINSIC DISORDER IN PROTEIN PRODUCTS OF NEWBORN GENES. [Thesis]. IUPUI; 2011. Available from: http://hdl.handle.net/1805/2681

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

28. Cepits, Judith Diane. A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA .

Degree: 1982, University of Arizona

URL: http://hdl.handle.net/10150/291379

Subjects/Keywords: RNA.; Genetic transcription.; Genetic engineering.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cepits, J. D. (1982). A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/291379

Chicago Manual of Style (16^th Edition):

Cepits, Judith Diane. “A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA .” 1982. Masters Thesis, University of Arizona. Accessed January 20, 2021. http://hdl.handle.net/10150/291379.

MLA Handbook (7^th Edition):

Cepits, Judith Diane. “A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA .” 1982. Web. 20 Jan 2021.

Vancouver:

Cepits JD. A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA . [Internet] [Masters thesis]. University of Arizona; 1982. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10150/291379.

Council of Science Editors:

Cepits JD. A MODEL FOR RNA SPLICING OF OVALBUMIN MESSENGER-RNA . [Masters Thesis]. University of Arizona; 1982. Available from: http://hdl.handle.net/10150/291379

University of South Carolina

29. Atilgan, Emrah. Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming.

Degree: PhD, Computer Science and Engineering, 2016, University of South Carolina

URL: https://scholarcommons.sc.edu/etd/3576

► Developing new materials have historically been time-consuming. Computational material discovery can search large design space to identify promising candidates for experimental verification. Recently, Density… (more)

▼ Developing new materials have historically been time-consuming. Computational material discovery can search large design space to identify promising candidates for experimental verification. Recently, Density Functional Theory (DFT) based first principle calculation has been able to calculate many electrical and physical properties of materials, making them suitable for computational doping based material discovery. In material doping, given a base material, one can change its properties by substituting some elements with new ones or adding additional elements. In computational doping, we have a grid of atoms in a supercell, some of which can be substituted with dopant atoms. There are many possible doping positions for the doped elements in the supercell, among which the most stable supercell with the lowest free electronic energy is the one that most likely appears in experiments. So finding the most stable doped supercell configuration is the first step for computational doping, which is usually done exhaustively nowadays. For each such substitution, the Vienna Ab-Initio Simulation Package is usually used to calculate its energy and higher level physicochemical properties. Free energy calculations take about 15-30 hours for a supercell of 75 atoms for substituting two positions out of 15 with a single dopant element, and it may take days to weeks for multiple dopant elements. This is a typical optimization problem with expensive evaluation functions. Here we first developed a genetic algorithm for finding the most stable structure of the doped material with the lowest free electronic energy for a single dopant element. It can reduce the running time for computational doping by up to 75%. We used SrTiO3 perovskite as the base material and Nb as the substitution element. We also developed another genetic algorithm for multiple dopant elements. Since the search space becomes larger, the genetic algorithm works better and saves up to 85% of calculations for finding the most stable structures. Finally, we developed a genetic programming (GP) algorithm for computational doping which can simultaneously determine multiple dopant elements with different doping ratios. The simultaneous search of dopant elements and their ratios can speed up the search process for large doping spaces. Advisors/Committee Members: Jianjun Hu.

Subjects/Keywords: Computer Sciences; Engineering; Computational Doping; Fuel Cell; Genetic Algorithms; Genetic Programming

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Atilgan, E. (2016). Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming. (Doctoral Dissertation). University of South Carolina. Retrieved from https://scholarcommons.sc.edu/etd/3576

Chicago Manual of Style (16^th Edition):

Atilgan, Emrah. “Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming.” 2016. Doctoral Dissertation, University of South Carolina. Accessed January 20, 2021. https://scholarcommons.sc.edu/etd/3576.

MLA Handbook (7^th Edition):

Atilgan, Emrah. “Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming.” 2016. Web. 20 Jan 2021.

Vancouver:

Atilgan E. Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming. [Internet] [Doctoral dissertation]. University of South Carolina; 2016. [cited 2021 Jan 20]. Available from: https://scholarcommons.sc.edu/etd/3576.

Council of Science Editors:

Atilgan E. Computational Doping for Fuel Cell Material Design Based on Genetic Algorithms and Genetic Programming. [Doctoral Dissertation]. University of South Carolina; 2016. Available from: https://scholarcommons.sc.edu/etd/3576

Oregon State University

30. Park, Eung-Jun. Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis.

Degree: PhD, Horticulture, 2005, Oregon State University

URL: http://hdl.handle.net/1957/23710

► Tomato [Lycopersicon esculentum Mill.] plants are chilling sensitive and do not naturally accumulate glycinebetaine (GB), a metabolite that functions as a stress protectant in plants.… (more)

▼ Tomato [Lycopersicon esculentum Mill.] plants are chilling sensitive and do not naturally accumulate glycinebetaine (GB), a metabolite that functions as a stress protectant in plants. While GB can increase tolerance of tomato plants to salt and drought stresses, its effect on chilling tolerance has not been examined. To evaluate whether GB improves chilling tolerance, tomato (cv. Moneymaker) plants were treated with foliar-applied GB. GB-treated plants exhibited enhanced chilling tolerance. During chilling treatment, GB-treated plants maintained lower H₂O₂ levels but a higher degree of catalase activity compared with the controls. The protective effect of GB on enhanced chilling tolerance disappeared within a week after the application. These results suggested genetic engineering of a biosynthetic GB pathway into tomato plants may provide constitutively enhanced chilling tolerance. Tomato plants (cv. Moneymaker) were transformed with a chloroplast-targeted codA gene of Arthrobacter globiformis, which encodes choline oxidase (COD) that catalyzes the conversion of choline to GB. Transgenic plants accumulated GB and their chloroplasts contain up to 86 % of total leaf GB. Over various developmental phases, transgenic plants were more tolerant of chilling stress than their wild-type (WT) counterparts. Transgenic plants also maintained lower H₂O₂ levels but a higher degree of catalase activity under chilling stress compared with the controls. Finally, GB accumulation in the chloroplasts of transgenic plants was positively correlated with their level of chilling tolerance, although GB levels in transgenic plants were very low compared to those in natural GB-accumulator plants. To increase the amount of GB in transgenic tomato plants, three codA expression cassettes were constructed to target the chloroplasts (Chl-codA), cytosol (Cyt-codA), or both locations (ChlCyt-codA). Targeting COD to the cytosol or to both locations resulted in higher GB accumulations in the Cyt-codA and ChlCyt-codA lines compared to the Chl-codA lines. Regardless of targeted location and the different amounts of GB, all three types exhibited similar degrees of enhanced tolerance to various abiotic stresses compared with the WT plants. The codA transgenic plants produced significantly enlarged flowers and fruits, which are a consequence of increased cell number and size. Fruit enlargement was caused by a pleiotropic effect of the codA gene transfer. Expression analysis revealed altered expression of genes that are involved in cell division. These results suggest that enlarged flowers and fruits are the pleiotropic effects of codA transgene expression, which may be useful for further improvement of these traits. All together, these results demonstrated that exogenous GB application enhanced chilling tolerance of non-transgenic tomato plants, and moreover introduction of a GB biosynthetic pathway into tomato confers enhanced tolerance to abiotic stresses, as well as increase in sizes of flowers and fruits. Advisors/Committee Members: Chen, Tony H. H. (advisor), Boyer, Charles D. (committee member).

Subjects/Keywords: Tomatoes – Genetic engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Park, E. (2005). Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/23710

Chicago Manual of Style (16^th Edition):

Park, Eung-Jun. “Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis.” 2005. Doctoral Dissertation, Oregon State University. Accessed January 20, 2021. http://hdl.handle.net/1957/23710.

MLA Handbook (7^th Edition):

Park, Eung-Jun. “Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis.” 2005. Web. 20 Jan 2021.

Vancouver:

Park E. Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis. [Internet] [Doctoral dissertation]. Oregon State University; 2005. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1957/23710.

Council of Science Editors:

Park E. Enhancement of cold tolerance in tomato plants by genetic engineering of glycinebetaine biosynthesis. [Doctoral Dissertation]. Oregon State University; 2005. Available from: http://hdl.handle.net/1957/23710

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations