subject:(gametogenesis)

University of Saskatchewan

1. Wang, Sheng. Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana.

Degree: 2018, University of Saskatchewan

► Protein ubiquitination, a fundamental protein modification cascade, is catalyzed by three types of enzymes referred to as E1 for ubiquitin (Ub) activation, E2 for Ub… (more)

▼ Protein ubiquitination, a fundamental protein modification cascade, is catalyzed by three types of enzymes referred to as E1 for ubiquitin (Ub) activation, E2 for Ub conjugation and E3 for Ub ligation. Arabidopsis has 37 E2 genes and functions for most of them remain largely unknown. Further, although the conventional Lys48-linked protein ubiquitination is well known for its function in protein degradation, functions for protein ubiquitination linked through other lysine residues of Ub, e.g. Lys11 and Lys63 are poorly understood in plants. This study aims to advance the understanding of the functions of Arabidopsis E2s involved in the unconventional ubiquitination. Previously, UBC13A/B have been identified as the E2 for Lys63-linked polyubiquitination, and ubc13 mutant plants display strong phenotypes in root development. In this study, a family of six E3s that can interact with UBC13 were identified, and when a subgroup of three genes were inactivated, the T-DNA mutants showed a glucose-sensitive phenotype suggesting their possible role in sugar signaling. It has been proposed that in root development UBC13 may control the level of Aux/IAA (Auxin/indole-3-acetic acid) proteins which regulate the activity of auxin response factors (ARFs). Several ARFs were evaluated, and overexpression of ARF6 enhanced the root hair growth in WT plants and partially rescued the root phenotypes of the ubc13 mutant. A major part of this study was on Arabidopsis UBC22, a sole member of an Arabidopsis E2 subfamily. Results from the phylogenetic analysis and in vitro ubiquitination assay indicate that UBC22 is an E2 for Lys11-linked ubiquitination in plants. The ubc22 knockout mutant had much reduced seed setting, and further analysis showed that UBC22 plays a critical role in the formation of functional megaspore and female gametophyte. Moreover, certain ubc22 mutant plants showed distinct phenotypes in vegetative development. The RNA-seq analysis revealed that genes of different pathways were differentially affected in different subtypes of plants. Plants of one subtype had increased expression of genes in pathogen defense and showed an enhanced resistance to a plant pathogen. Therefore, the present results have provided a new understanding on the function of UBC13, identified UBC22 as a novel E2, and revealed its multiple functions in plants. Advisors/Committee Members: Wang, Hong, Datla, Raju, Lee, Jeremy, Wei, Yangdou, Wu, Yuliang.

Subjects/Keywords: Ubiquitination; gametogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, S. (2018). Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/11670

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Sheng. “Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana.” 2018. Thesis, University of Saskatchewan. Accessed March 08, 2021. http://hdl.handle.net/10388/11670.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Sheng. “Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana.” 2018. Web. 08 Mar 2021.

Vancouver:

Wang S. Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10388/11670.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang S. Studies of E2s Related to Unconventional Ubiquitination in Arabidopsis thaliana. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/11670

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

2. Russell, Stewart. The Piwi Pathway in Bovine Gametes and Preimplantation Embryos.

Degree: PhD, Department of Biomedical Sciences, 2016, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9895

► Epigenetic reprogramming is required during both embryo and gamete development to restore the potential for cellular pluripotency. Changes in DNA methylation during reprogramming can allow… (more)

▼ Epigenetic reprogramming is required during both embryo and gamete development to restore the potential for cellular pluripotency. Changes in DNA methylation during reprogramming can allow the expression of integrated endogenous transposable elements (TEs) that may lead to genomic instability should widespread reintegration occur. Small RNA mediated mechanisms have therefore evolved to constrain TE expression during reprogramming. PIWI proteins are a subfamily of the Argonaute clade of proteins which have functions in small RNA (sRNA) mediated TE and gene silencing. PIWI proteins direct silencing through RNA-mediated interactions with complementary nucleic acid targets. TE expression has been documented in early bovine embryos, although mechanisms regulating their expression are not widely studied. This period is further complicated by extensive targeted decay of maternally-derived mRNAs which may utilize related mechanisms of gene silencing. Based on these observations, it was hypothesized that the PIWI pathway is present in bovine gametes and embryos, and regulates endogenous TEs and mRNAs during reprogramming. PIWIL1 expression was characterized in bovine gonads, oocytes, and embryos. The testis-specific expression of full-length PIWIL1 showed localization to spermatocytes and spermatids, and association with piRNAs of 29-32 nucleotides in length. Additionally, two shorter, 3ʹ truncated PIWIL1 transcript isoforms were found to be expressed in testis, oocytes and embryos, and showed dynamic regulation throughout embryogenesis. Full-length PIWIL2 and PIWIL3 transcripts were also quantified throughout embryogenesis. To address potential roles for the PIWI pathway, sRNAs from oocytes and zygotes were profiled through next-generation sequencing and compared to those from testis, ovary and sperm. An analysis “pipeline” was developed to identify putative piRNAs from the sequencing libraries. The piRNA-“like” RNAs (pilRNAs) were characterized based on their size and canonical biogenesis features and were abundant in oocytes and embryos. When mapped to bovine repeats, up to one third of embryonic pilRNAs targeted TE classes including LINEs, SINEs, and ERVs. Through comparisons with published embryonic mRNA transcriptomes this analysis also revealed the potential for maternally-deposited, zygotic pilRNAs to direct mRNA degradation. These data provide one of the first characterizations of the PIWI pathway outside of classical models, and provide evidence for novel functions in embryo TE and gene regulation. Advisors/Committee Members: LaMarre, Jonathan (advisor).

Subjects/Keywords: piRNA; Piwi; Embryo; Preimplantation Development; Epigenetics; Gametogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Russell, S. (2016). The Piwi Pathway in Bovine Gametes and Preimplantation Embryos. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9895

Chicago Manual of Style (16^th Edition):

Russell, Stewart. “The Piwi Pathway in Bovine Gametes and Preimplantation Embryos.” 2016. Doctoral Dissertation, University of Guelph. Accessed March 08, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9895.

MLA Handbook (7^th Edition):

Russell, Stewart. “The Piwi Pathway in Bovine Gametes and Preimplantation Embryos.” 2016. Web. 08 Mar 2021.

Vancouver:

Russell S. The Piwi Pathway in Bovine Gametes and Preimplantation Embryos. [Internet] [Doctoral dissertation]. University of Guelph; 2016. [cited 2021 Mar 08]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9895.

Council of Science Editors:

Russell S. The Piwi Pathway in Bovine Gametes and Preimplantation Embryos. [Doctoral Dissertation]. University of Guelph; 2016. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9895

University of Arizona

3. MYLES, ELBERT LEWIS, JR. CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS).

Degree: 1985, University of Arizona

URL: http://hdl.handle.net/10150/187915

► Plants monosomic for chromosome 9 of Gossypium hirsutum, in addition to Haplo 9 plants, produce many kinds of aneuploid progenies consisting of monosomics, trisomics, multiple… (more)

▼ Plants monosomic for chromosome 9 of Gossypium hirsutum, in addition to Haplo 9 plants, produce many kinds of aneuploid progenies consisting of monosomics, trisomics, multiple monosomics, and different monosomic-trisomic combinations. Cytological analysis of megasporagenesis was conducted to determine the stage and the mechanism that would account for the production of the different kinds of aneuploid progeny. Two new cytotypes involving chromosome 9 were isolated and identified as monotelodisome 9S (Telo 9S) and monoisodisome 9S (Iso 9S), both of which produce different kinds of aneuploids very similar to Haplo 9. Monotelodisomic 9L plants do not produce various kinds of aneuploid progeny like Haplo 9, Telo 9S and Iso 9S. These observations indicate that control of chromosome segregation is on the long arm of chromosome 9. There was no significant difference between Telo 9S and Iso 9S in the kinds and frequencies of aneuploids, but there was a significant difference between these two and Haplo 9. Haplo 9, Telo 9S and Iso 9S showed no significant difference in the chromosomes subtracted or added to the chromosome complement of their respective aneuploid progenies. Analysis of female gametogenesis in TM1 or control plants showed that premeiotic division occurred when bud size is 6.0 - 6.6mm; meiosis occurred when bud size is 6.3 - 7.2mm; the first mitotic division of the megaspore occurred when bud size is 6.9 - 7.8mm, and the second and third mitotic divisions of the megaspore occurred when bud size is 7.8 - 8.5mm. The premeiotic, meiotic, and post meiotic divisions were normal. Female gametogenesis was cytologically analyzed in Haplo 9 plants and it was observed that there was a higher frequency of gametophytes with fewer post meiotic divisions and that there was nonsynchrony of the post-meiotic divisions in a number of the developing female gametophytes. It is assumed that these irregularities are due to the absence of chromosome 9. It is concluded that the long arm of chromosome 9 has control over chromosome segregation, and that most likely nondisjunction occurs throughout female gametogenesis, but confined primarily to the three mitotic divisions following meiosis. Genetic tests showed that eight mutant genes were not located on chromosome 9. The aneuploids had lower seeds per boll than normal disomic plants. Advisors/Committee Members: Endrizzi, John E (advisor).

Subjects/Keywords: Gametogenesis.; Aneuploidy.; Cottonseed.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MYLES, ELBERT LEWIS, J. (1985). CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS). (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/187915

Chicago Manual of Style (16^th Edition):

MYLES, ELBERT LEWIS, JR. “CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS). ” 1985. Doctoral Dissertation, University of Arizona. Accessed March 08, 2021. http://hdl.handle.net/10150/187915.

MLA Handbook (7^th Edition):

MYLES, ELBERT LEWIS, JR. “CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS). ” 1985. Web. 08 Mar 2021.

Vancouver:

MYLES, ELBERT LEWIS J. CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS). [Internet] [Doctoral dissertation]. University of Arizona; 1985. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10150/187915.

Council of Science Editors:

MYLES, ELBERT LEWIS J. CYTOGENETICS OF GAMETOGENESIS IN HAPLO 9 OF GOSSYPIUM HIRSUTUM L. (MEGASPOROGENESIS, MEIOSIS). [Doctoral Dissertation]. University of Arizona; 1985. Available from: http://hdl.handle.net/10150/187915

4. McDermond, John. Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA.

Degree: 2014, Nova Southeastern University

URL: http://nsuworks.nova.edu/occ_stuetd/17

► Sexual reproduction in Porites divaricata (Le Sueur, 1820) was studied over a one year period (August, 2012 to August, 2013) in a shallow water (~2m… (more)

Subjects/Keywords: Porites; Brooder; Fragmentation; Scleractinian spawning; Gametogenesis; Temperature; Marine Biology; Oceanography

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APA (6^th Edition):

McDermond, J. (2014). Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA. (Thesis). Nova Southeastern University. Retrieved from http://nsuworks.nova.edu/occ_stuetd/17

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McDermond, John. “Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA.” 2014. Thesis, Nova Southeastern University. Accessed March 08, 2021. http://nsuworks.nova.edu/occ_stuetd/17.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McDermond, John. “Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA.” 2014. Web. 08 Mar 2021.

Vancouver:

McDermond J. Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA. [Internet] [Thesis]. Nova Southeastern University; 2014. [cited 2021 Mar 08]. Available from: http://nsuworks.nova.edu/occ_stuetd/17.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McDermond J. Reproduction and Population of Porites divaricata at Rodriguez Key: The Florida Keys, USA. [Thesis]. Nova Southeastern University; 2014. Available from: http://nsuworks.nova.edu/occ_stuetd/17

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Charmpilas, Nikolaos. Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans.

Degree: 2018, University of Crete (UOC); Πανεπιστήμιο Κρήτης

URL: http://hdl.handle.net/10442/hedi/45979

►

The C. elegans germline recapitulates mammalian stem cell niches, and has proven instrumental in understanding key aspects of stem cell biology. However, the molecular and… (more)

▼

The C. elegans germline recapitulates mammalian stem cell niches, and has proven instrumental in understanding key aspects of stem cell biology. However, the molecular and physiological requirements for germline stem cell homeostasis remain largely elusive. We investigated the role of mitochondrial biogenesis and function in the preservation of germline stem cell identity. Here, we show that general transcription activity in germline mitochondria is highly compartmentalized and parallels mitochondrial maturation. Expression of RPOM-1, the mitochondrial RNA polymerase, increases as germ nuclei progress from the distal to the proximal gonadal arm to form oocytes, and is directly regulated by IFET-1, a translational repressor required for normal P granule formation. Mitochondria transition from globular to tubular morphology and become polarized, as they approach the proximal gonad arm. Notably, we find that a similar transition and temporal mitochondrial RNA polymerase expression profile characterizes differentiation of mammalian stem cells. This shift is accompanied by increased ATP and ROS production. Perturbation of mitochondrial bioenergetics causes gonad syncytium hyperplasia by disrupting the balance between mitosis and differentiation to oocytes, resulting in a marked reduction of fecundity. Consequently, compensatory apoptosis is induced in the germline. Sperm-derived signals promote mitochondrial maturation and germ cell differentiation via the MEK/ERK kinase pathway. Germ cell fate decisions are determined by a crosstalk between Insulin/IGF-1 and TGF-β signaling, mitochondria and protein synthesis. Our findings demonstrate that a shift in mitochondrial bioenergetics guides germline stem cell differentiation, and implicate mitochondrial transcription in germ cell differentiation and germline tumor development.

Η γαμετική σειρά του C. elegans προσομοιάζει τους βλαστικούς θύλακες των θηλαστικών και έχει αποδειχτεί ανεκτίμητης αξίας για την κατανόηση βασικών πτυχών της βιολογίας των βλαστικών κυττάρων. Παρόλα αυτά, πολλά από τα μοριακά και φυσιολογικά προαπαιτούμενα για τη διατήρηση της ομοιόστασης των γαμετικών βλαστικών κυττάρων παραμένουν άγνωστα. Ερευνήσαμε το ρόλο της μιτοχονδριακής βιογένεσης και λειτουργίας στην διατήρηση της ταυτότητας των γαμετικών βλαστικών κυττάρων. Η δουλειά μας καταδεικνύει πως η μιτοχονδριακή μεταγραφή στα γαμετικά μιτοχόνδρια είναι διαμερισματοποιημένη και έρχεται παράλληλα με την μιτοχονδριακή ωρίμανση. Η έκφραση της RPOM-1, της μιτοχονδριακής RNA πολυμεράσης, αυξάνεται καθώς οι γαμετικοί πυρήνες μεταβαίνουν από τον άπω στον εγγύς βραχίονα της γονάδας και σχηματίζουν ωοκύτταρα. Η έκφραση της RPOM-1 ρυθμίζεται από τον IFET-1, ένα μεταφραστικό καταστολέα και συστατικό των P granules. Τα μιτοχόνδρια αυτά καθ’ αυτά μεταβαίνουν από μια σφαιρική σε μια επιμήκη μορφολογία και πολώνονται καθώς πλησιάζουν τον εγγύς βραχίονα της γονάδας και η παραγωγή ATP και ROS αυξάνεται. Μια αντίστοιχη μετάβαση και αλλαγή στην έκφραση της μιτοχονδριακής RNA πολυμεράσης χαρακτηρίζει και την…

Subjects/Keywords: DNA, μιτοχονδριακό (M+DNA); Γαμετογένεση; DNA, mitochondrial; Gametogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Charmpilas, N. (2018). Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans. (Thesis). University of Crete (UOC); Πανεπιστήμιο Κρήτης. Retrieved from http://hdl.handle.net/10442/hedi/45979

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Charmpilas, Nikolaos. “Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans.” 2018. Thesis, University of Crete (UOC); Πανεπιστήμιο Κρήτης. Accessed March 08, 2021. http://hdl.handle.net/10442/hedi/45979.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Charmpilas, Nikolaos. “Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans.” 2018. Web. 08 Mar 2021.

Vancouver:

Charmpilas N. Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans. [Internet] [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10442/hedi/45979.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Charmpilas N. Ένας μιτοχονδριακός ροοστάτης καθοδηγεί τη διαφοροποίηση των γαμετικών βλαστικών κυττάρων στον Caenorhabditis elegans. [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2018. Available from: http://hdl.handle.net/10442/hedi/45979

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Maine

6. Feehan, Keri. Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords.

Degree: MS, Marine Biology, 2016, University of Maine

URL: https://digitalcommons.library.umaine.edu/etd/2433

► The purpose of this study was to determine the basic reproductive biology and seasonality of the Patagonian fjord coral Desmophyllum dianthus. Desmophyllum dianthus is… (more)

▼ The purpose of this study was to determine the basic reproductive biology and seasonality of the Patagonian fjord coral Desmophyllum dianthus. Desmophyllum dianthus is a deep-sea solitary scleractinian found throughout the world’s oceans and an important benthic habitat builder. The Chilean Patagonian fjords are the only known location where this species occurs >50 m and thus are the only place to collect samples efficiently, effectively and economically. Corals were collected via SCUBA approximately every three months (when conditions permitted) from August 2012 to September 2013 from three sites within the Northern Patagonian fjords – Lilihuape (n=76) and Punta Huinay (n=59) in the Comau fjord, and Punta Mamurro (n=44) in Reñihue fjord. The objectives of this study were to determine sexuality, reproductive mode, oocyte size, fecundity and seasonality using histological techniques. Environmental data (temperature, salinity and light) was also analyzed to compare to seasonal reproductive trends and bring reproductive data into context. This study determined that Desmophyllum dianthus is dioecious (gonochoristic), having both male and female individuals. This species is also highly seasonal, spawning in the austral winter (August) and beginning gamete production in early spring. The fjord was coolest and most saline in August 2012, potentially cueing spawning. No planula larvae were found in any of the 8,000 histological sections. Due to the presence of late stage oocytes in August 2012, it is likely D. dianthus’s mode of reproduction is spawning rather than brooding. However this distinction could not be determined in this study. Oogenesis starts in September producing previtellogenic oocytes (size range: 25 – 200 μm) that slowly developed into vitellogenic oocytes by June. Vitellogenic oocytes ranged from 200 – 380 μm. Fecundity is relatively high compared to other deep-sea scleractinians, ranging from 2,448 (± 5.13 SE) to 172,328 (±103.67 SE) average potential oocytes per polyp. This research provides the first insight into Desmophyllum dianthus reproductive biology and yields an important baseline for continuing work on this benthic habitat builder in the Chilean fjords, and in the deep ocean. Advisors/Committee Members: Rhian G. Waller, Kevin Eckelbarger, Robert Steneck.

Subjects/Keywords: Cold-water Coral; Scleractinian; Reproduction; Seasonality; Gametogenesis; Fjord; Marine Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Feehan, K. (2016). Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords. (Masters Thesis). University of Maine. Retrieved from https://digitalcommons.library.umaine.edu/etd/2433

Chicago Manual of Style (16^th Edition):

Feehan, Keri. “Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords.” 2016. Masters Thesis, University of Maine. Accessed March 08, 2021. https://digitalcommons.library.umaine.edu/etd/2433.

MLA Handbook (7^th Edition):

Feehan, Keri. “Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords.” 2016. Web. 08 Mar 2021.

Vancouver:

Feehan K. Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords. [Internet] [Masters thesis]. University of Maine; 2016. [cited 2021 Mar 08]. Available from: https://digitalcommons.library.umaine.edu/etd/2433.

Council of Science Editors:

Feehan K. Highly Seasonal Reproduction in Desmophyllum dianthus from the Northern Patagonian Fjords. [Masters Thesis]. University of Maine; 2016. Available from: https://digitalcommons.library.umaine.edu/etd/2433

University of Maine

7. Rossin, Ashley. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.

Degree: MS, Marine Biology, 2018, University of Maine

URL: https://digitalcommons.library.umaine.edu/etd/2866

► Primnoa pacifica, otherwise known as the Red Tree Coral, is one of the most ecologically important corals in the North Pacific. This species is… (more)

▼ Primnoa pacifica, otherwise known as the Red Tree Coral, is one of the most ecologically important corals in the North Pacific. This species is an ecosystem engineer, providing essential habitat for commercially important fish and invertebrate species. Ocean acidification (OA) threatens corals and all calcifying organisms and is more prevalent in polar and sub-polar regions as the concentration of CO₂ is higher and there is a lowered buffering capacity due to low alkalinity in colder waters. The impact from a chemical shift in the oceans could alter the role of P. pacifica as an ecosystem engineer in predicted ocean conditions. An experiment was conducted to assess the impacts of OA on the gametogenesis of Red Tree Corals from Tracy Arm fjord in Alaska. Primnoa pacifica colonies were cultured for six to nine months in either projected 2100 pH (7.55 pH units) or Ambient pH (7.75 pH units), then were prepared for histology to investigate any changes in gametogenesis in acidified water. Oocyte diameters and fecundities were significantly lower in the 2100 samples. The females from the experiment also had a higher proportion of individuals experiencing reabsorption of vitellogenic oocytes, potentially to preserve the lipids for other ecological processes as a stress response. The highest percentage of oosorption was seen in the 2100 females, as well as the smallest oocytes and the lowest fecundities. There was a “null tank effect” observed in all measurement types, however these only significantly affected the analyses of the exterior measurements and spermatogenesis. When compared to a published 2014 reproductive dataset, all the tank samples were statistically different from previous years and were significantly lower. These results indicate that reproduction in this species may not be possible in the current pH predictions for 2100, or that if spawning could still occur, any spawned oocyte may not be sufficiently equipped to support a larva. This study only investigated one life history stage of these long-lived organisms and more research spanning multiple life history stages needs to be done in their natural habitat as well as in homogeneous laboratory conditions. There is also a need to investigate the probability of these corals spawning under environmental stressors to identify if these important species will be present in the future. Advisors/Committee Members: Rhian Waller, Bob Steneck, Aaron Strong.

Subjects/Keywords: Gulf of Alaska; gametogenesis; Primnoa; histology; oogenesis; ocean acidification; Marine Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rossin, A. (2018). The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. (Masters Thesis). University of Maine. Retrieved from https://digitalcommons.library.umaine.edu/etd/2866

Chicago Manual of Style (16^th Edition):

Rossin, Ashley. “The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.” 2018. Masters Thesis, University of Maine. Accessed March 08, 2021. https://digitalcommons.library.umaine.edu/etd/2866.

MLA Handbook (7^th Edition):

Rossin, Ashley. “The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.” 2018. Web. 08 Mar 2021.

Vancouver:

Rossin A. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. [Internet] [Masters thesis]. University of Maine; 2018. [cited 2021 Mar 08]. Available from: https://digitalcommons.library.umaine.edu/etd/2866.

Council of Science Editors:

Rossin A. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. [Masters Thesis]. University of Maine; 2018. Available from: https://digitalcommons.library.umaine.edu/etd/2866

University of Edinburgh

8. Sousa Martins, Joao Pedro. Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis.

Degree: PhD, 2012, University of Edinburgh

URL: http://hdl.handle.net/1842/9893

► Gametogenesis is a highly complex process that requires stringent control of gene expression, in which translational regulation plays an essential role. Deleted in Azoospermia-like (DAZL)… (more)

▼ Gametogenesis is a highly complex process that requires stringent control of gene expression, in which translational regulation plays an essential role. Deleted in Azoospermia-like (DAZL) belongs to the DAZ family of RNA-binding proteins, which are restricted to germ cells, and regulate mRNA translation. Importantly, loss of function of these proteins results in infertility in both males and females in a wide variety of organisms. A model for the mechanism by which DAZL stimulates translation has been proposed based on work in Xenopus laevis (X. laevis) oocytes. In this model, DAZL functions by recruiting the translation initiation factor poly(A)-binding protein (PABP) to the 3’ untranslated region (UTR) of messenger RNAs. Simultaneous binding of PABP to Dazl and factors at the 5’ end confers a “closed-loop” mRNA conformation, which promotes translation initiation. To examine whether DAZL plays a similar role in mammals, co-expression of Dazl and PABP family members was investigated in fetal and adult mouse gonads. In contrast to X. laevis, mammals encode four cytoplasmic PABPs which share a similar domain organisation: PABP1, tPABP, ePABP and PABP4, of which PABP1 and PABP4 appear to be expressed in a wide range of tissues. Immunohistochemistry revealed that Dazl, Pabp1 and Pabp4 are all expressed in primordial germ cells (PGCs) but these show different expression patterns following germ cell sex differentiation. In adult testes Dazl is expressed in spermatogonia and spermatocytes, coinciding with the peak of Pabp4 expression. In contrast, the peak of Pabp1 expression occurs later than that of Dazl, with these proteins only being co-expressed in late pachytene and secondary spermatocyte phases. In adult ovaries, Pabp1, Pabp4 and Dazl are all expressed in the oocytes of primordial and primary follicles. Since both PABP family members are co-expressed with Dazl, the ability of DAZL to interact with PABP1 and PABP4 was investigated in vitro and in vivo. Surprisingly, these studies showed that DAZL discriminates between different PABP family members, only interacting with PABP1, providing the first report of a PABP-specific protein partner. Several putative DAZL mutations have been identified in patients with impaired fertility. Two of these mutations, I37A and R115G, are located in the RNA recognition motif (RRM), a domain which is found in many RNA-binding proteins and mediates both RNA and protein interactions. Thus, the role of these mutations in the ability of DAZL to stimulate translation was investigated. To this end, a translational target of human DAZL (hDAZL) was sought. The 3’UTR of growth differentiation factor 9 (hGDF9) mRNA was found to confer regulation by hDAZL and thus the ability of mutant DAZLs to stimulate reporter mRNAs containing this 3’UTR was examined. This revealed that both mutations compromised the ability of hDAZL to stimulate hGDF9 translation, suggesting a causative effect. These results were further confirmed in assays in which hDAZL is artificially tethered to mRNAs. The ability of mutant…

Subjects/Keywords: 572.8; DAZL; Deleted in Azoospermia-like; gametogenesis; PABP

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APA (6^th Edition):

Sousa Martins, J. P. (2012). Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/9893

Chicago Manual of Style (16^th Edition):

Sousa Martins, Joao Pedro. “Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis.” 2012. Doctoral Dissertation, University of Edinburgh. Accessed March 08, 2021. http://hdl.handle.net/1842/9893.

MLA Handbook (7^th Edition):

Sousa Martins, Joao Pedro. “Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis.” 2012. Web. 08 Mar 2021.

Vancouver:

Sousa Martins JP. Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2012. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1842/9893.

Council of Science Editors:

Sousa Martins JP. Functional analysis of DAZL-mediated translation activation during mammalian gametogenesis. [Doctoral Dissertation]. University of Edinburgh; 2012. Available from: http://hdl.handle.net/1842/9893

University of Iowa

9. Brockway, Heather Marie. A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression.

Degree: PhD, Genetics, 2014, University of Iowa

URL: https://ir.uiowa.edu/etd/1296

► Defects in meiotic prophase I events, resulting in aneuploidy, are a leading cause of birth defects in humans; however, these are difficult to study… (more)

▼ Defects in meiotic prophase I events, resulting in aneuploidy, are a leading cause of birth defects in humans; however, these are difficult to study in mammalian systems due to their occurrence very early in development. The nematode, Caenorhabditis elegans, is an excellent model for prophase I studies as its gonad is temporally and spatially organized around these meiotic events. Homolog pairing, synapsis, meiotic recombination and crossover formation are essential to the proper segregation of chromosomes into the respective gametes, either the egg or sperm. Disturbances in these events leads to missegregation of chromosomes in the gametes in the meiotic divisions. Synapsis is especially critical in meiosis as it precedes and is required for meiotic recombination in C. elegans. The formation of the synaptonemal complex (SC) is fundamental to chromosomal synapsis, yet the molecular mechanisms of synaptonemal complex morphogenesis are largely unknown. The investigations described in this thesis were undertaken to better understand the molecular contributions to synaptonemal complex morphogenesis. Chapter One reviews knowledge of morphogenesis and its relationship to the events of meiotic prophase I. Recent studies in our laboratory have implicated AKIRIN, a nuclear protein with multiple biological functions, as having a role in synaptonemal complex disassembly, specifically preventing the aggregation of synaptonemal proteins (Clemons et al., 2013). As a result of our efforts to discern the mechanism by which AKIRIN regulates disassembly, we found that the highly conserved CSN/COP9 signalosome has a role in SC assembly, leading to defects in prophase I events and in MAPK signaling , leading to the arrest of nuclei in the later stages of meiosis. While the CSN/COP9 signalosome has been implicated in general fertility in C. elegans (Pintard et al., 2003), no role had been defined in earlier meiotic stages until this study. Chapter Two describes an RNAi enhancer/suppressor screen undertaken in the akir-1 mutant background. Several RNAi clones were selected for future study based on a reduction in brood size; one of which, csn-5/, is the focus of the analysis presented in Chapter 3. Chapter Three describes the phenotypic characterization of two CSN/COP9 signalosome subunits, csn-2 and csn-5. Alleles of both genes display synaptonemal complex protein aggregation and defects in mitotic cell proliferation, homologous chromosome pairing, meiotic recombination and crossover formation, leading to an increase in apoptosis. Oocyte maturation is also disrupted by a lack of MAPK signaling, resulting in a lack of viable oocytes, which renders the csnmutant homozygotes sterile. These findings support a model suggesting the CSN/COP9 signalosome has an essential role in regulating meiotic prophase I events and oocyte maturation. Chapter 4 describes the methodology used in this study. Chapter 5 provides a summary of the thesis findings and… Advisors/Committee Members: Smolikove, Sarit (supervisor).

Subjects/Keywords: C elegans; CSN/COP9 signalosome; Gametogenesis; Meiosis; Reproductive Biology; Genetics

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APA (6^th Edition):

Brockway, H. M. (2014). A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1296

Chicago Manual of Style (16^th Edition):

Brockway, Heather Marie. “A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression.” 2014. Doctoral Dissertation, University of Iowa. Accessed March 08, 2021. https://ir.uiowa.edu/etd/1296.

MLA Handbook (7^th Edition):

Brockway, Heather Marie. “A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression.” 2014. Web. 08 Mar 2021.

Vancouver:

Brockway HM. A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression. [Internet] [Doctoral dissertation]. University of Iowa; 2014. [cited 2021 Mar 08]. Available from: https://ir.uiowa.edu/etd/1296.

Council of Science Editors:

Brockway HM. A role for the CSN/COP9 signalosome in synaptonemal complex assembly and meiotic progression. [Doctoral Dissertation]. University of Iowa; 2014. Available from: https://ir.uiowa.edu/etd/1296

University of Oregon

10. Oates, Mark. Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864).

Degree: MS, Department of Biology, 2013, University of Oregon

URL: http://hdl.handle.net/1794/13329

► From January 2012 to December 2012 I collected adult oysters from two intertidal populations on a monthly basis in the Coos Bay estuary, Oregon for… (more)

Subjects/Keywords: condition index; gametogenesis; Olympia oyster; Ostrea lurida; reproductive cycle; site comparison

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APA (6^th Edition):

Oates, M. (2013). Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864). (Masters Thesis). University of Oregon. Retrieved from http://hdl.handle.net/1794/13329

Chicago Manual of Style (16^th Edition):

Oates, Mark. “Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864).” 2013. Masters Thesis, University of Oregon. Accessed March 08, 2021. http://hdl.handle.net/1794/13329.

MLA Handbook (7^th Edition):

Oates, Mark. “Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864).” 2013. Web. 08 Mar 2021.

Vancouver:

Oates M. Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864). [Internet] [Masters thesis]. University of Oregon; 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1794/13329.

Council of Science Editors:

Oates M. Observations of Gonad Structure and Gametogenic Timing in a Recovering Population of Ostrea lurida (Carpenter 1864). [Masters Thesis]. University of Oregon; 2013. Available from: http://hdl.handle.net/1794/13329

University of Victoria

11. Rossi, Giulia. The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions.

Degree: Department of Biology, 2016, University of Victoria

URL: http://hdl.handle.net/1828/7650

► Oceanic uptake of anthropogenic CO2 emissions is causing wholesale shifts seawater carbonate chemistry towards a state of decreased carbonate ion concentration and reduced ocean pH.… (more)

▼ Oceanic uptake of anthropogenic CO2 emissions is causing wholesale shifts seawater carbonate chemistry towards a state of decreased carbonate ion concentration and reduced ocean pH. This change in water chemistry has potentially dire implications for marine organisms, especially those that build and maintain calcium carbonate structures. Our understanding of how ocean acidification may affect marine organisms is limited, as most studies have been short-term laboratory experiments. The CO2 flux from hydrothermal vent fluids on NW Eifuku submarine volcano (Mariana Volcanic Arc) provides a natural setting to investigate the effects of acidification. Here, the vent mussel, Bathymodiolus septemdierum, lives in water with pH as low as 5.22. This study was designed to examine the consequences of a low pH environment on reproduction, calcification and somatic growth in B. septemdierum, since the presumed elevated cost of acid-base regulation may diminish available energy for these processes. Histological analysis reveals both females and males display synchronous gametogenesis across collection sites with spawning occurring between late winter and early spring. Mussels are functionally dioecious, although evidence of protogynous hermaphroditism was found– a first record for the genus. In comparison with mussels at near normal pH, we find no evidence that the pattern of gametogenesis is affected by low pH conditions. However, calcification is compromised: at a given shell volume, shells from NW Eifuku weigh about half those from sites with near normal pH mussels. The condition index (CI = body ash free dry weight/ shell volume) was assessed in mussels collected from four low pH sites on Northwest Eifuku and two control sites from Lau Basin and Nifonea Ridge; we show that low pH conditions negatively affect CI, especially when energy availability is limited. Bathymodiolus septemdierum acquires energy from chemoautotrophic symbionts in the specialized gill epithelial cells. Using a gill condition index (GCI = gill ash free dry weight/ shell volume) and transmission electron microscopy to determine symbiont abundances in gill tissues, we show that NW Eifuku mussels with healthy gills and abundant symbionts have a higher CI than mussels from NW Eifuku with unhealthy gills. Optimal environmental sulphide concentrations appear to sustain higher symbiont abundances. While the survival of mussels on NW Eifuku is remarkable, it can come at a considerable cost to body and shell condition when during periods of energy limitation. Bathymodiolus septemdierum shows substantial resilience to low pH conditions when energy availability is sufficient due to energy budget adjustments that maximize fitness. Advisors/Committee Members: Tunnicliffe, Verena (supervisor).

Subjects/Keywords: Bathymodiolus septemdierum; Northwest Eifuku; High CO2; Gametogenesis,; Protogyny; Body condition; Endosymbionts

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APA (6^th Edition):

Rossi, G. (2016). The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/7650

Chicago Manual of Style (16^th Edition):

Rossi, Giulia. “The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions.” 2016. Masters Thesis, University of Victoria. Accessed March 08, 2021. http://hdl.handle.net/1828/7650.

MLA Handbook (7^th Edition):

Rossi, Giulia. “The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions.” 2016. Web. 08 Mar 2021.

Vancouver:

Rossi G. The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions. [Internet] [Masters thesis]. University of Victoria; 2016. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1828/7650.

Council of Science Editors:

Rossi G. The reproductive and physiological condition of a deep-sea mussel (Bathymodiolus septemdierum Hashimoto & Okutani, 1994) living in extremely acidic conditions. [Masters Thesis]. University of Victoria; 2016. Available from: http://hdl.handle.net/1828/7650

12. Saraiva Garcia, Carlos Henrique. Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches.

Degree: Docteur es, Pathologie moléculaire, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066582

►

Le paludisme est causé par des parasites du genre Plasmodium et la gamétogenèse est une étape essentielle à sa transmission. Afin d'améliorer les connaissances sur… (more)

▼

Le paludisme est causé par des parasites du genre Plasmodium et la gamétogenèse est une étape essentielle à sa transmission. Afin d'améliorer les connaissances sur la genèse des gamètes, nous avons mené des études protéomiques sur des gamétocytes de P. berghei sauvages ou mutants avec le gène moteur de la kinésine 8 interrompu. Le mutant est morphologiquement semblable au type sauvage mais incapable de compléter la gamétogenèse et les gamètes mâles d'exflageller. A partir d'échantillons enrichis en gamétocytes, la gamétogenèse a été suivie sur 15 min après induction par l'acide xanthurénique. Les peptides marqués par iTRAQ à partir de triplicatas biologiques ont été analysés par nanoLC/MS-MS et interprétés par les logiciels Patternlab et Blast2GO. Les phosphopeptides ont été enrichis au TiO2. Chez le parasite sauvage, 443 protéines et 206 phosphoprotéines ont été identifiées à partir de 2617 peptides et chez le mutant, 530 protéines et 218 phosphoprotéines à partir de 3198 peptides. L'induction de la gamétogenèse est marquée par une transcription, une biosynthèse de protéines et de l'ADN importantes. Chez le mutant, la formation des fuseaux mitotiques et des axonèmes des flagelles des gamètes mâles est fortement affectée. Des protéines du complexe du protéasome dépendant de l'ubiquitine, de la réponse au stress, du métabolisme énergétique sont également affectées. Cette étude apporte de nouveaux éléments de compréhension sur le rôle joué par la kinésine 8 dans la gamétogenèse de Plasmodium.

Malaria is caused by parasites from Plasmodium genus and its gametogenesis is an essential step to ensure the malaria transmission. In efforts to improve the knowledge on gamete biology we did quantitative proteomic and phosphoproteomic comparative experiments with P. berghei WT and gametocytes disrupted for the motor gene kinesin 8. The mutant is morphologically similar to wild-type parasite but impaired gametogenesis and unable to exflagellate. The Gametocytes-enriched sample from mice at time T0 were xanthurenic acid-induced to exflagellate and harvested at time T7 and T15. The iTRAQ labelled peptides from independent biological triplicate sample were analyzed overtime through nanoLC/MS-MS Orbitrap after TiO2 enrichment, and interpreted by Patternlab and Blast2GO softwares. The wild type had 443 proteins and 206 phosphoproteins identified from 2,617 peptides. The mutant had 530 proteins and 218 phosphoproteins identified from 3,198 peptides. GO biological processes related to RNA translation, DNA and protein biosynthesis were most prominent and phosphorylated proteins are mainly RNA, ATP or protein binding proteins. Within the mutant, the axoneme and mitotic spindle microtubules disorganization were strongly affected. The nucleosome components are key to nuclear division disorganization. The ubiquitin-dependent proteasome complex and stress/folding response, energy metabolism and egress proteins were affected. The Plasmodium proteomic approach brings that insight into kinesin 8 critical importance for male gametogenesis in…

Advisors/Committee Members: Charneau, Sébastien (thesis director), Grellier, Philippe (thesis director).

Subjects/Keywords: Plasmodium; Gametogenèse; Exflagellation; Protéome; Phosphopeptides; Kinésine; Plasmodium; Gametogenesis; Exflagellation; 616.9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saraiva Garcia, C. H. (2016). Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066582

Chicago Manual of Style (16^th Edition):

Saraiva Garcia, Carlos Henrique. “Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 08, 2021. http://www.theses.fr/2016PA066582.

MLA Handbook (7^th Edition):

Saraiva Garcia, Carlos Henrique. “Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches.” 2016. Web. 08 Mar 2021.

Vancouver:

Saraiva Garcia CH. Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2016PA066582.

Council of Science Editors:

Saraiva Garcia CH. Etude des événements moléculaires de la gamétogenèse de Plasmodium berghei par des approches protéomiques : Investigation of the molecular events in Plasmodium berghei gametogenesis through proteomic approaches. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066582

13. Maouche, Ahmed. Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss).

Degree: Docteur es, Écologie et évolution, 2018, Rennes, Agrocampus Ouest

URL: http://www.theses.fr/2018NSARA083

►

Chez les mammifères, les cellules souches germinales primordiales nommées PGC sont soumises à différents microenvironnements successifs au sein de la gonade mâle qui permettent leur… (more)

▼

Chez les mammifères, les cellules souches germinales primordiales nommées PGC sont soumises à différents microenvironnements successifs au sein de la gonade mâle qui permettent leur transition progressive d’abord en prospermatogonies avant la naissance puis en cellules souches spermatogoniales (SSC) chez l’adulte. Chez les poissons, l’existence potentielle d’états de transition intermédiaires entre les PGC et les SSC reste à être démontrée. De même, les mécanismes moléculaires impliqués dans la construction du pool initial de SSC ou dans son maintien à l’âge adulte restent mal compris. Le premier volet de la thèse a eu pour objectif d’étudier des voies de régulations susceptibles d’être impliquées dans le processus de formation, de renouvèlement ou de recrutement des SSC chez la truite arc-en-ciel (Oncorhynchus mykiss).Le profil d’expression spatiotemporelle des gènes codant pour les ligands Kit, Gdnf, endothéline (Edn) et leurs récepteurs respectifs suggère que ces gènes pourraient agir successivement sur les SSC au cours de l’ontogenèse de la truite. A la différence des mammifères, les facteurs kit et gdnf pourraient agir à la fois de manière paracrine et autocrine. L’hormone folliculo-stimulante et les stéroïdes sexuels pourraient réguler les voies gdnf et edn. Le deuxième volet de la thèse a eu pour objectif d’améliorer la praticabilité de la méthode de transplantation des SSC pour régénérer des ressources génétiques précieuses. Nous montrons que la transplantation de SSC non purifiées dans la cavité abdominale d’embryons de truites « golden » triploïdes permet, chez

In mammals, Primordial Germ Cells (PGCs) are subject to sucessive microenvironments that allow their progressive transition first, into prospermatogonia at pre-natal stage and then into spermatogonial stem cells (SSC) in adults. In fish, the potential existence of intermediate transition states between PGC and SSC remains to be demonstrated. Likewise, the molecular mechanisms involved in the construction of the initial SSC pool or in its maintenance in adulthood remain poorly understood. The firUnlike mammals, the extracellular Kit and Gdnf ligands are duplicated and they could act as paracrine or autocrine regulatory factors. The follicle stimulating hormone and sex steroid hormones could regulate the gdnf/gfra1 and edn/ednr regulatory pathways. The second part of the thesis was aimed to improve the practicability of the SSC transplantation technique to regenerate valuable genetic resources in European aquaculture research infrastructures. We show that the transplantation of unpurified SSC into the abdominal cavity of triploid "golden" trout embryos allows, in approximately 70% of the injected animals, a satisfactory production of functional gametes derived solely from transplanted SSC

Advisors/Committee Members: Lareyre, Jean-Jacques (thesis director).

Subjects/Keywords: Ssc; Expressions des gènes; Gamétogenèse; Transplantation; Ssc; Gene expression; Gametogenesis; Transplantation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maouche, A. (2018). Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss). (Doctoral Dissertation). Rennes, Agrocampus Ouest. Retrieved from http://www.theses.fr/2018NSARA083

Chicago Manual of Style (16^th Edition):

Maouche, Ahmed. “Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss).” 2018. Doctoral Dissertation, Rennes, Agrocampus Ouest. Accessed March 08, 2021. http://www.theses.fr/2018NSARA083.

MLA Handbook (7^th Edition):

Maouche, Ahmed. “Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss).” 2018. Web. 08 Mar 2021.

Vancouver:

Maouche A. Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss). [Internet] [Doctoral dissertation]. Rennes, Agrocampus Ouest; 2018. [cited 2021 Mar 08]. Available from: http://www.theses.fr/2018NSARA083.

Council of Science Editors:

Maouche A. Caractérisation de voies de régulation paracrine potentiellement impliquées dans le devenir des cellules souches spermatogoniales chez la truite arc-en-ciel (Oncorhynchus mykiss) : Characterization of Paracrine Regulatory Pathways Potentially Involved in the Fate of Spermatogonial Stem Cells in Rainbow Trout (Oncorhynchus mykiss). [Doctoral Dissertation]. Rennes, Agrocampus Ouest; 2018. Available from: http://www.theses.fr/2018NSARA083

University of Toronto

14. Xu, Mengshu. Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/68360

► Histone H3 lysine 4 methylation (H3K4me) is a well-studied chromatin modification that is associated with active transcription; however, its physiological roles are not well understood.… (more)

Subjects/Keywords: chromatin; gametogenesis; histone methylation; mitochondria; Saccharomyces cerevisiae; transcription; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, M. (2014). Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/68360

Chicago Manual of Style (16^th Edition):

Xu, Mengshu. “Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase.” 2014. Doctoral Dissertation, University of Toronto. Accessed March 08, 2021. http://hdl.handle.net/1807/68360.

MLA Handbook (7^th Edition):

Xu, Mengshu. “Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase.” 2014. Web. 08 Mar 2021.

Vancouver:

Xu M. Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1807/68360.

Council of Science Editors:

Xu M. Developmental Control Throughout the Budding Yeast Life Cycle by Jhd2, a JARID family Histone H3 Lysine 4 Demethylase. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/68360

Louisiana State University

15. Honig, Aaron Jacob. Population ecology of the ribbed mussel in southeastern Louisiana.

Degree: MS, Environmental Sciences, 2013, Louisiana State University

URL: etd-04152013-152031 ; https://digitalcommons.lsu.edu/gradschool_theses/3906

► Benthic intertidal bivalves play an essential role in estuarine ecosystems by contributing to habitat provision, water filtration, and promoting productivity. As such, ecosystem level changes… (more)

▼ Benthic intertidal bivalves play an essential role in estuarine ecosystems by contributing to habitat provision, water filtration, and promoting productivity. As such, ecosystem level changes that impact population distributions and persistence of local bivalve populations may have large ecosystem level consequences, making it important to better understand the population distribution and ecology of native bivalve populations. Gametogenesis, recruitment, growth, mortality, size structure and density of the ribbed mussel, Geukensia demissa, were examined across a salinity gradient in southeastern Louisiana. In summer 2012, 100-m transects were placed at interior and edge marsh plots to collect data on mussel density, and vegetation data at duplicate sites in upper (salinity ~4), central (salinity ~8) and lower (salinity ~15) Barataria Bay, LA. Caged growth and recruitment plots were established adjacent to each transect, in April, and growth, mortality and recruitment of individually marked mussels within plots were recorded in November 2012. Mussels were randomly sampled monthly from low (~ 5) and high (~25) salinity marsh sites, and histologically processed to determine the seasonal progression of gametogenesis. Mussel densities were greatest within mesohaline marsh (66.6 + 18 m-2), J. roemerianus vegetation (191.2 + 42.7 m-2) and plots experiencing 20-60% annual flooding rates (46.7 + 13.8 m-2). Mussel recruitment, growth, size and survival were significantly higher at mid and high salinity marsh edge sites as compared to all interior and low salinity sites. Peak gametogenic ripeness occurred between April and September, positively correlated with temperature, and coincidental with seasonal shifts in salinity. The observed patterns of density, growth and mortality in Barataria Bay may reflect detrital food resource availability due to local site flooding rates, vegetation community distribution along the salinity gradient, and reduced predation at higher salinity edge sites.

Subjects/Keywords: recruitment; gametogenesis; mortality; growth; density; Juncus; Spartina; salinity; Geukensia; ribbed mussel

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APA (6^th Edition):

Honig, A. J. (2013). Population ecology of the ribbed mussel in southeastern Louisiana. (Masters Thesis). Louisiana State University. Retrieved from etd-04152013-152031 ; https://digitalcommons.lsu.edu/gradschool_theses/3906

Chicago Manual of Style (16^th Edition):

Honig, Aaron Jacob. “Population ecology of the ribbed mussel in southeastern Louisiana.” 2013. Masters Thesis, Louisiana State University. Accessed March 08, 2021. etd-04152013-152031 ; https://digitalcommons.lsu.edu/gradschool_theses/3906.

MLA Handbook (7^th Edition):

Honig, Aaron Jacob. “Population ecology of the ribbed mussel in southeastern Louisiana.” 2013. Web. 08 Mar 2021.

Vancouver:

Honig AJ. Population ecology of the ribbed mussel in southeastern Louisiana. [Internet] [Masters thesis]. Louisiana State University; 2013. [cited 2021 Mar 08]. Available from: etd-04152013-152031 ; https://digitalcommons.lsu.edu/gradschool_theses/3906.

Council of Science Editors:

Honig AJ. Population ecology of the ribbed mussel in southeastern Louisiana. [Masters Thesis]. Louisiana State University; 2013. Available from: etd-04152013-152031 ; https://digitalcommons.lsu.edu/gradschool_theses/3906

University of Pennsylvania

16. Bryant, Jessica Michelle. Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae.

Degree: 2014, University of Pennsylvania

URL: https://repository.upenn.edu/edissertations/1216

► Gametogenesis is a complex process that results in a highly differentiated gamete capable of transmitting genetic and epigenetic information to the next generation. In the… (more)

▼ Gametogenesis is a complex process that results in a highly differentiated gamete capable of transmitting genetic and epigenetic information to the next generation. In the cases of mammalian spermatogenesis and yeast sporulation, an extreme post-meiotic compaction of the genome is key to gamete function. While genome compaction in sperm is reliant upon a histone-to-protamine transition, yeast spores accomplish compaction with a full complement of histones. Although the mechanisms behind such striking chromatin dynamics are largely unknown, several histone variants and post-translational modifications, especially acetylation of histone H4, have been implicated in these processes. The following studies elucidate the roles of two proteins, BRD4 and the linker histone (Hho1), in chromatin compaction during mouse spermatogenesis and yeast sporulation, respectively. In the post-meiotic phase of mouse spermatogenesis, BRD4 forms a ring structure around the haploid nucleus at the cytoskeletal base of the developing acrosome, which has been implicated in nuclear compaction and shaping. Chromatin immunoprecipitation followed by mass spectrometry and sequencing in post-meiotic cells revealed that BRD4, a bromodomain-containing protein, binds to acetylated histone H4 and is enriched in intergenic regions of the genome where histone replacement by protamines is most predominant in mature sperm. Thus, BRD4 may provide a structural link between the contractile force of acrosome formation and the removal of acetylated histones from the genome. In contrast to sperm, spores must use a histone-based chromatin compaction mechanism. During sporulation, Hho1 plays a dual role: transcriptional repression and nuclear compaction. Hho1 is depleted during meiosis and enriched in post-meiotic spore chromatin. Moreover, Hho1 shows a high genome-wide binding correlation with Ume6, the master repressor of meiotic genes. Meiotic depletion of both of these proteins may lead to the activation of key sporulation genes. In addition, knockout of HHO1 revealed its necessity in meiotic progression and post-meiotic genome compaction. These data provide support to the interesting hypothesis that protamines are evolutionarily derived from linker histones: Hho1 may play the role of protamines in yeast. Taken together, these studies in mouse and yeast highlight the complexity of mechanisms developed in diverse eukaryotic systems to facilitate the compaction of the gamete genome.

Subjects/Keywords: BRD4; chromatin; gametogenesis; histone; spermatogenesis; sporulation; Cell Biology; Molecular Biology

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APA (6^th Edition):

Bryant, J. M. (2014). Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae. (Thesis). University of Pennsylvania. Retrieved from https://repository.upenn.edu/edissertations/1216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bryant, Jessica Michelle. “Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae.” 2014. Thesis, University of Pennsylvania. Accessed March 08, 2021. https://repository.upenn.edu/edissertations/1216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bryant, Jessica Michelle. “Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae.” 2014. Web. 08 Mar 2021.

Vancouver:

Bryant JM. Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae. [Internet] [Thesis]. University of Pennsylvania; 2014. [cited 2021 Mar 08]. Available from: https://repository.upenn.edu/edissertations/1216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bryant JM. Chromatin Compaction and Genome Reorganization During Spermatogenesis in M. Musculus and Sporulation in S. Cerevisiae. [Thesis]. University of Pennsylvania; 2014. Available from: https://repository.upenn.edu/edissertations/1216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

17. Reynolds, Thomas Leonard. An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development.

Degree: PhD, Graduate School, 1982, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487175195908409

Subjects/Keywords: Biology; Hyoscyamus niger; Pollen; Gametogenesis

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APA (6^th Edition):

Reynolds, T. L. (1982). An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487175195908409

Chicago Manual of Style (16^th Edition):

Reynolds, Thomas Leonard. “An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development.” 1982. Doctoral Dissertation, The Ohio State University. Accessed March 08, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487175195908409.

MLA Handbook (7^th Edition):

Reynolds, Thomas Leonard. “An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development.” 1982. Web. 08 Mar 2021.

Vancouver:

Reynolds TL. An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development. [Internet] [Doctoral dissertation]. The Ohio State University; 1982. [cited 2021 Mar 08]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487175195908409.

Council of Science Editors:

Reynolds TL. An ultrastructural and stereological analysis of pollen grains of Hyoscyamus niger during male gametogenesis and induced embryogenic development. [Doctoral Dissertation]. The Ohio State University; 1982. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487175195908409

Leiden University

18. Gomes, Fernandes M. A day in the life of a germ cell - on gametogenesis and pluripotency.

Degree: 2018, Leiden University

URL: http://hdl.handle.net/1887/66721

► In this thesis, several critical time-points of human germ cell development are addressed.In Chapter 2, we adopted a culture system combining features of human embryonicstem… (more)

Subjects/Keywords: gametogenesis; BMP signalling; PIWIL proteins; gametogenesis; BMP signalling; PIWIL proteins

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APA (6^th Edition):

Gomes, F. M. (2018). A day in the life of a germ cell - on gametogenesis and pluripotency. (Doctoral Dissertation). Leiden University. Retrieved from http://hdl.handle.net/1887/66721

Chicago Manual of Style (16^th Edition):

Gomes, Fernandes M. “A day in the life of a germ cell - on gametogenesis and pluripotency.” 2018. Doctoral Dissertation, Leiden University. Accessed March 08, 2021. http://hdl.handle.net/1887/66721.

MLA Handbook (7^th Edition):

Gomes, Fernandes M. “A day in the life of a germ cell - on gametogenesis and pluripotency.” 2018. Web. 08 Mar 2021.

Vancouver:

Gomes FM. A day in the life of a germ cell - on gametogenesis and pluripotency. [Internet] [Doctoral dissertation]. Leiden University; 2018. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1887/66721.

Council of Science Editors:

Gomes FM. A day in the life of a germ cell - on gametogenesis and pluripotency. [Doctoral Dissertation]. Leiden University; 2018. Available from: http://hdl.handle.net/1887/66721

19. Muramatsu, Daniela. Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro.

Degree: Mestrado, Zoologia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/41/41133/tde-16102007-132551/ ;

►

Uma das espécies de coral pétreo zooxantelado com mais ampla distribuição no litoral brasileiro é Madracis decactis Lyman, 1859. M. decactis forma colônias incrustantes nodulares… (more)

▼

Uma das espécies de coral pétreo zooxantelado com mais ampla distribuição no litoral brasileiro é Madracis decactis Lyman, 1859. M. decactis forma colônias incrustantes nodulares que podem atingir até 30 cm de diâmetro. O estudo da gametogênese foi realizado através de coletas bimensais na Baía de Ilha Grande, RJ durante 21 meses (agosto/2004-maio/2006), totalizando 10 coletas (12 colônias/coleta) (Licença IBAMA no. 201/2004). Foram realizados cortes histológicos de 7 µm, e de 10 até 16 pólipos por colônia foram analisados, totalizando mais de 1800 pólipos. A análise dos pólipos indicou que M. decactis é hermafrodita, com gametas localizados no mesmo lóculo gástrico, porém em mesentérios diferentes. A gametogênese durou cerca de sete meses. A ovogênese iniciou-se ao redor de outubro, enquanto que a espermatogênese teve inicio no final de fevereiro, ambas terminando em sincronia no final do mês de maio. O exame dos pólipos férteis indicou a presença dos estágios I, II e III de desenvolvimento para a ovogênese e dos estágios I, II, III e IV para a espermatogênese. Não foram encontrados embriões ou plânulas nos cortes histológicos, indicando talvez que estes estágios permaneçam pouco tempo no interior do pólipo. O pico da atividade reprodutiva ocorre entre os meses de fevereiro e abril com todas as colônias férteis contendo ovócitos principalmente no estágio III de maturação. A provável época de liberação de plânulas ocorre entre os meses de abril e maio, sob influencia das condições ambientais como a temperatura da água do mar, a irradiação solar e a pluviosidade. O presente trabalho forneceu informações básicas a respeito da biologia reprodutiva de Madracis decactis presente em Ilha Grande, sendo uma contribuição para outros estudos mais específicos na área de manejo e conservação de ambientes marinhos.

One of the most wide distributed coral species along the Brazilian cost is Madracis decactis Lyman, 1859. M. decactis growths as nodular incrusting colonies that can reach up to 30 cm in diameter. In order to study the gametogenesis cycle, bimonthly collections were done at Ilha Grande Bay, Rio de Janeiro, during 21 months (August/2004- May/2006), totaling 10 collections (12 colonies/collection) (License no. 201/2004). Histological sections of 7 µm thick were done to analyze 10 to 16 polyps per colony (more than 1800 polyps in total). The analysis indicated that M. decactis is hermaphroditic, with male and female gametes developing at the same gastric loculi, but in different mesenteries. The gametogenesis lasted about 7 months, the oogenesis starts at October , while spermtogenesis starts at the end of February, both reaching the maturity in synchrony at the end of May. The exam of fertile polyps indicated the presence of stages I, II and III for oogenesis and I, II, III and IV for spermatogenesis. No embryo or planula were observed in the histological sections, indicating that maybe these stages stay for a short period inside the polyp cavity. The peak of reproductive activity was between February and April when…

Advisors/Committee Members: Silveira, Fabio Lang da.

Subjects/Keywords: Madracis decactis; Madracis decactis; Cnidaria; Cnidaria; Gametogênese; gametogenesis; Reprodução; Reproduction; Scleractinia; Scleractinia

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APA (6^th Edition):

Muramatsu, D. (2007). Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/41/41133/tde-16102007-132551/ ;

Chicago Manual of Style (16^th Edition):

Muramatsu, Daniela. “Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro.” 2007. Masters Thesis, University of São Paulo. Accessed March 08, 2021. http://www.teses.usp.br/teses/disponiveis/41/41133/tde-16102007-132551/ ;.

MLA Handbook (7^th Edition):

Muramatsu, Daniela. “Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro.” 2007. Web. 08 Mar 2021.

Vancouver:

Muramatsu D. Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Mar 08]. Available from: http://www.teses.usp.br/teses/disponiveis/41/41133/tde-16102007-132551/ ;.

Council of Science Editors:

Muramatsu D. Quantificação da gametogênese através de análises histológicas para estimar a reprodução sexuada de Madracis decactis Lyman, 1859 (Cnidaria, Anthozoa, Scleractinia) do litoral sul do Estado do Rio de Janeiro. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/41/41133/tde-16102007-132551/ ;

20. Rinaldi, Vera Da Silva Garcia. Genetically dissecting the meiotic checkpoint active during prophase I in female mice.

Degree: PhD, Comparative Biomedical Sciences, 2017, Cornell University

URL: http://hdl.handle.net/1813/56716

► Females have a non-renewable number of gametes at birth. These oocytes are extremely sensitive to environmental factors that generate DNA damage. Oocyte death due to… (more)

▼ Females have a non-renewable number of gametes at birth. These oocytes are extremely sensitive to environmental factors that generate DNA damage. Oocyte death due to DNA damage can result in infertility and ovarian failure. In contrast to postnatal oocytes, at earlier stages of gametogenesis these cells withstand hundreds of developmentally programmed DNA breaks (DSBs). During the first meiotic division these DSBs promote synapsis (homologous chromosomes pairing), and recombination, which are both essential for sexual reproduction and environmental fitness. However, DSB repair and synapsis need to occur in a timely manner, or the quality of the gametes becomes compromised. The mechanisms that guarantee oocyte quality were hypothesized to operate through two independent pathways: one that surveys DNA integrity, and the other synapsis. However, I present experimental evidence that oocytes defective for either DNA repair or synapsis are eliminated by the same DNA damage response. Furthermore, through the detailed analysis of DNA repair dynamics, I provide evidence that the protein HORMAD2, which localizes to unsynapsed chromosomes, regulates DSB-repair. I hypothesize that HORMAD2 interferes with repair by preventing broken DNA from using the sister chromatid as a repair template. This “block to sister-chromatid repair” (BSCR) assures that the homologous chromosome is the substrate of choice. Whereas BSCR guarantees homologous recombination, it also prevents unsynapsed chromosomes from fixing DSBs. Thus, failure to synapse will result in persistent DSBs. Since DNA damage causes oocyte death postnatally, unsynapsed chromosome will trigger the DNA damage checkpoint. Through the understanding of this checkpoint, I was able to test if the transient inhibition of the DNA damage checkpoint protein (CHK2) prevents oocyte death. My finding that oocyte death was prevented, and fertility was preserved, provides evidence that chemically protecting oocyte from DNA damaging agents is a viable clinical approach. This result will hopefully translate into a treatment to delay ovarian failure. Taken together these results have implications on our current understanding of the prophase I checkpoint. TEACHING AS RESEARCH My interest in improving teaching strategies led me to research the qualitative outcome of using a novel teaching tool during the laboratory section of a histology course. I tested an interactive response system (IRS) as formative assessment tool. I found that IRS results in a positive experience, however my study was not able to detect quantitative difference on students’ grades was detected. Advisors/Committee Members: Schimenti, John C. (chair), Coonrod, Scott A. (committee member), Alani, Eric E. (committee member), Cohen, Paula (committee member).

Subjects/Keywords: teaching; Developmental biology; meiosis; Biology; Genetics; reproduction; DNA-damage checkpoint; fertility; gametogenesis

10 more images…

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APA (6^th Edition):

Rinaldi, V. D. S. G. (2017). Genetically dissecting the meiotic checkpoint active during prophase I in female mice. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/56716

Chicago Manual of Style (16^th Edition):

Rinaldi, Vera Da Silva Garcia. “Genetically dissecting the meiotic checkpoint active during prophase I in female mice.” 2017. Doctoral Dissertation, Cornell University. Accessed March 08, 2021. http://hdl.handle.net/1813/56716.

MLA Handbook (7^th Edition):

Rinaldi, Vera Da Silva Garcia. “Genetically dissecting the meiotic checkpoint active during prophase I in female mice.” 2017. Web. 08 Mar 2021.

Vancouver:

Rinaldi VDSG. Genetically dissecting the meiotic checkpoint active during prophase I in female mice. [Internet] [Doctoral dissertation]. Cornell University; 2017. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1813/56716.

Council of Science Editors:

Rinaldi VDSG. Genetically dissecting the meiotic checkpoint active during prophase I in female mice. [Doctoral Dissertation]. Cornell University; 2017. Available from: http://hdl.handle.net/1813/56716

21. Lata, Sneh. Studies on morphology gametogenesis and egg shell formation of some cestodes;.

Degree: Zoology, 2015, Chaudhary Charan Singh University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/34145

None newline

Refrences p. 129-157, Summary p. 122-127

Advisors/Committee Members: Pandey, K C.

Subjects/Keywords: Morphology Gametogenesis; Egg Shell Formation; Cestodes

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APA (6^th Edition):

Lata, S. (2015). Studies on morphology gametogenesis and egg shell formation of some cestodes;. (Thesis). Chaudhary Charan Singh University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/34145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lata, Sneh. “Studies on morphology gametogenesis and egg shell formation of some cestodes;.” 2015. Thesis, Chaudhary Charan Singh University. Accessed March 08, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/34145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lata, Sneh. “Studies on morphology gametogenesis and egg shell formation of some cestodes;.” 2015. Web. 08 Mar 2021.

Vancouver:

Lata S. Studies on morphology gametogenesis and egg shell formation of some cestodes;. [Internet] [Thesis]. Chaudhary Charan Singh University; 2015. [cited 2021 Mar 08]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/34145.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lata S. Studies on morphology gametogenesis and egg shell formation of some cestodes;. [Thesis]. Chaudhary Charan Singh University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/34145

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Germain, Devon Roy. From Gametogenesis to Oncogenesis: The Many Roles of DDX1.

Degree: PhD, Department of Oncology, 2015, University of Alberta

URL: https://era.library.ualberta.ca/files/cz316q178s

► DEAD box proteins are a family of RNA helicases implicated in every aspect of RNA biogenesis and metabolism. Modification of RNA secondary structure by DEAD… (more)

▼ DEAD box proteins are a family of RNA helicases implicated in every aspect of RNA biogenesis and metabolism. Modification of RNA secondary structure by DEAD box proteins has widespread effects on numerous cellular processes due to the ubiquitous nature of RNA. While the biochemical activity of several DEAD box proteins has been described, there is limited information into the biological functions of most family members. DEAD box 1 (DDX1) is over-expressed in some cancers, and has in vitro RNA/RNA and RNA/DNA unwinding activity. In vivo analyses have implicated DDX1 in RNA maturation, double-stranded DNA damage response and viral maturation. To date, there have been no reported DDX1-negative cell lines, with neuronal and germline cells showing the highest DDX1 levels. Here, we describe three novel facets of DDX1 biology and function. First, we report that DDX1 is a novel and independent prognostic indicator in breast cancer. Both increased DDX1 mRNA levels and cytoplasmic DDX1 protein localization are negative prognostic markers. Analysis of treatment-specific subgroups revealed that this effect is elevated in patients who receive adjuvant therapy, indicating that DDX1 may be predictive of patient response to treatment. Second, we report the generation of a novel Ddx1-null allele in Drosophila melanogaster. Ddx1-null flies are viable, but have phenotypes consistent with reduced metabolism and display aberrant gametogenesis. We also describe an interaction between Ddx1 and Sirup mRNA, which may underlie the metabolic disruption observed in mutant Ddx1 flies. Third, we have found that Ddx1-/- mice die at the pre-blastocyst stage. We also observed that mice carrying one Ddx1- allele display transgenerational inheritance, with heterozygous Ddx1 mice generated from heterozygous intercrosses producing significantly fewer wild-type progeny than expected. We attribute this to a novel modification of the wild-type allele in heterozygous mice and demonstrate that transgenerational inheritance of Ddx1 is distinct from previously described cases of transgenerational epigenetic inheritance. These combined studies address different aspects of DDX1 function, providing new insight into the spectrum of biological roles associated with DDX1. DDX1 appears to be under strict regulation, as evidenced by the fact that Ddx1 levels in mice do not vary between heterozygous and wild-type animals. Strict regulation of DDX1 levels may explain why prognostic effects are observed when DDX1 levels are elevated in breast cancer. Although we did not attempt to determine why high levels of DDX1 are associated with early recurrence, the phenotypes observed in our Ddx1-null flies suggest a role for Ddx1 in regulating metabolism. We propose that DDX1 is an integral regulator of metabolism and growth, and is itself tightly regulated. As such, deregulation of DDX1 can either promote tumourigenesis (when over-expressed) or death (when absent). This model of DDX1 action indicates that it may be possible to target DDX1 to improve patient outcome in those…

Subjects/Keywords: DDX1; Gametogenesis; Oncogenesis; Transgenerational epigenetics

…25 1.4.2 Gametogenesis… …REDUCED SIZE AND ABERRANT GAMETOGENESIS .................................................... 73… …Gametogenesis is disrupted in Ddx1-null flies............................... 91 3.3.4 Ddx1-null… …18 Figure 1.4 Drosophila gametogenesis… …spermatogenesis defects (Sugimoto et al. 2009). In addition to its role in gametogenesis…

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APA (6^th Edition):

Germain, D. R. (2015). From Gametogenesis to Oncogenesis: The Many Roles of DDX1. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cz316q178s

Chicago Manual of Style (16^th Edition):

Germain, Devon Roy. “From Gametogenesis to Oncogenesis: The Many Roles of DDX1.” 2015. Doctoral Dissertation, University of Alberta. Accessed March 08, 2021. https://era.library.ualberta.ca/files/cz316q178s.

MLA Handbook (7^th Edition):

Germain, Devon Roy. “From Gametogenesis to Oncogenesis: The Many Roles of DDX1.” 2015. Web. 08 Mar 2021.

Vancouver:

Germain DR. From Gametogenesis to Oncogenesis: The Many Roles of DDX1. [Internet] [Doctoral dissertation]. University of Alberta; 2015. [cited 2021 Mar 08]. Available from: https://era.library.ualberta.ca/files/cz316q178s.

Council of Science Editors:

Germain DR. From Gametogenesis to Oncogenesis: The Many Roles of DDX1. [Doctoral Dissertation]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/cz316q178s

Universidade do Rio Grande do Sul

23. Silvério, Adriano. Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar.

Degree: 2009, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/76772

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O presente trabalho analisou as etapas de formação de rudimentos seminais e grãos de pólen de Passiflora elegans e P. haematostigma. As espécies foram analisadas… (more)

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O presente trabalho analisou as etapas de formação de rudimentos seminais e grãos de pólen de Passiflora elegans e P. haematostigma. As espécies foram analisadas fazendo-se o uso de microscopia fotônica e microscopia eletrônica de varredura e transmissão. O gineceu apresenta um estigma com emergências estigmáticas, estilete sólido e ovário tricarpelar e unilocular. O ovário apresenta inúmeros rudimentos seminais que formam-se a partir de divisões periclinais da camada central. Os rudimentos são crassinucelados e os tegumentos têm origem dérmica. A célula arquesporial divide-se mitoticamente antes da meiose. A meiose resulta em uma tétrade linear, com ginósporo calazal viável. A ginogametogênese é do tipo Poligonum e, durante a diferenciação do ginófito, os estratos parietais do nucelo são parcialmente consumidos e a epiderme nucelar divide-se periclinalmente na porção apical. As antípodas são efêmeras e o ginófito maduro apresenta duas sinérgides com aparelho fibrilar desenvolvido, oosfera e núcleo secundário no pólo micropilar. O rudimento seminal é anátropo, arilado, bitegumentado e com micrópila em “zig-zag”. O androceu é constituído por cinco anteras tetrasporangiadas. Os estratos parietais desenvolvem-se do tipo Dicotiledôneo e as camadas médias são persistentes na antera madura de P. haematostigma. As células do tapete são poliplóides e colapsam liberando os conteúdos no interior do lóculo. A citocinese é do tipo simultânea e as tétrades são tetraédricas na maioria. O pólen é liberado na fase bicelular, apresenta número de colpos variável em P. elegans e seis colporos em P. haematostigma. A esporoderme não apresenta camada basal em P. elegans e é mais espessa do que em P. haematostigma. Plastídios e mitocôndrias são englobados durante a formação da célula generativa e persistem até a fase de pólen maduro.

The present work analyzed the different developmental stages of the ovule and of the pollen grain in Passiflora elegans and P. haematostigma. The species were analyzed using photonic microscopy and scanning and transmission electron microscopy. The gynoecium has a stigma with stigmatic outgrowths, solid style and tricarpelar and unilocular ovary. The ovary has several seminal rudiments that are originated from the periclinal divisions of the central layer. The ovule is crassinucelate and the dermal layer originates the integuments. The arquesporial cell divides mitotically before meiosis. The meiosis results into a linear tetrad and only the chalazal gynospore is viable. The gynogametogenesis is of the Poligonum type, the nucellus parietal layers are partially degraded during the gynophyte differentiation, and nuclear epidermis divides periclinally in the apical portion. The antipodal cells are ephemeral and the mature gynophyte has two synergids with a developed filiform apparatus, egg cell and secondary nuclei in the micropilar pole. The ovule is anatropous, arillate, bitegmic and with zig-zag micropile. The androecium is constituted of five tetrasporangiate anthers. The parietal layers develop like the…

Advisors/Committee Members: Mariath, Jorge Ernesto de Araujo.

Subjects/Keywords: Passiflora; Embriologia vegetal : Anatomia vegetal : Citologia vegetal : Teses; Passifloraceae; Sporogenesis; Gametogenesis; Pollen; Plastidial inheritance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Silvério, A. (2009). Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/76772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Silvério, Adriano. “Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar.” 2009. Thesis, Universidade do Rio Grande do Sul. Accessed March 08, 2021. http://hdl.handle.net/10183/76772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Silvério, Adriano. “Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar.” 2009. Web. 08 Mar 2021.

Vancouver:

Silvério A. Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2009. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10183/76772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Silvério A. Aspectos embriológicos de espécies do gênero Passiflora (Passifloraceae), com ênfase no potencial de herança organelar. [Thesis]. Universidade do Rio Grande do Sul; 2009. Available from: http://hdl.handle.net/10183/76772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of South Florida

24. Kanwar, Ankush. Studies Aimed at the Synthesis of Anti-Infective Agents.

Degree: 2018, University of South Florida

URL: https://scholarcommons.usf.edu/etd/7176

► Infectious diseases continue to be a major concern worldwide. They are the second leading cause of death after heart disease. Factors such as an increasing… (more)

▼ Infectious diseases continue to be a major concern worldwide. They are the second leading cause of death after heart disease. Factors such as an increasing global population, travel, urbanization, global climate change and evolution of pathogens have made infectious diseases more common. Infectious diseases, particularly neglected tropical diseases (NTDs) result in many deaths worldwide. Malaria and leishmaniasis are two common (NTDs) which affect low income countries around the globe. Low cost drugs with novel mechanism of action are required to tackle the growing resistances of parasites against current drugs used in the developing world, where most of the cases occur. The first part of this manuscript (chapters 1 - 3) describes the synthesis of novel analogs active against Leishmania donovani parasite which causes leishmaniasis. Leishmaniasis is a vector-borne complex group of diseases transmitted through the bite of an infected female sand-fly. Its clinical manifestations range from the less severe (cutaneous) to fatal (visceral) forms depending upon infecting species, immunity of host and the environment. Reports have suggested the role of Heat shock protein 90 (Hsp 90) in the differentiation of the Leishmania parasite from the promastigote stage to the pathogenic amastigote stage inside the host. A series of tetrahydro-indazole, tetrahydro-pyrazolo pyridine and radicicol hybrid compounds were prepared based on known Hsp 90 inhibitors, SNX2112 and NVP-AUY922. The synthetic approach allowed us to generate a diverse library of analogs which were used to probe the hydrophobic pocket of Hsp 90 active site. The most active compound, was found to be twice more active as the clinically used drug, Miltefosine, in an infected macrophage assay with an IC50 = 0.88 µM. The second part of this manuscript (chapters 4 - 5) describes the synthesis of xanthurenic acid analogs as antimalarial drugs. Xanthurenic acid (XA) is a vital component for the gametogenesis of the Plasmodium inside the mosquito’s gut. Gametogenesis plays an important part in the continuation of the parasite’s life cycle. A series of xanthurenic acid analogs were synthesized with the aim of inducing premature exflagellation of the microgametes, thus blocking the key step required for the transmission of parasites from humans to the mosquito. A biotinylated xanthurenic acid analog and a clickable xanthurenic acid analog were also synthesized which will help us investigate the mechanism of action of xanthurenic acid in inducing gametogenesis in mosquito. In the preliminary screening efforts in an exflagellation assay, analog 4.40 showed promising activity and was more active in inducing exflagellation than xanthurenic acid. An exflagellation assay of other analogs is currently being pursued. Further investigations into the molecular target and mechanism of action are underway with the biotinylated xanthurenic acid analog.

Subjects/Keywords: Infectious diseases; Leishmania donovani; promastigotes; amastigotes; radicicol; Plasmodium; xanthurenic acid; gametogenesis; Chemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kanwar, A. (2018). Studies Aimed at the Synthesis of Anti-Infective Agents. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/7176

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kanwar, Ankush. “Studies Aimed at the Synthesis of Anti-Infective Agents.” 2018. Thesis, University of South Florida. Accessed March 08, 2021. https://scholarcommons.usf.edu/etd/7176.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kanwar, Ankush. “Studies Aimed at the Synthesis of Anti-Infective Agents.” 2018. Web. 08 Mar 2021.

Vancouver:

Kanwar A. Studies Aimed at the Synthesis of Anti-Infective Agents. [Internet] [Thesis]. University of South Florida; 2018. [cited 2021 Mar 08]. Available from: https://scholarcommons.usf.edu/etd/7176.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kanwar A. Studies Aimed at the Synthesis of Anti-Infective Agents. [Thesis]. University of South Florida; 2018. Available from: https://scholarcommons.usf.edu/etd/7176

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

25. Henley, William F. Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae).

Degree: PhD, Fisheries and Wildlife Sciences, 2002, Virginia Tech

URL: http://hdl.handle.net/10919/27932

► To determine the effects of different algal diets on freshwater mussels, tissues of Elliptio complanata were sampled for physiological, somatic, and gametogenic condition from August… (more)

▼ To determine the effects of different algal diets on freshwater mussels, tissues of Elliptio complanata were sampled for physiological, somatic, and gametogenic condition from August 1999 to May 2000. Treatments included mussels fed Scenedesmus quadricauda (S), Neochloris oleoabundans (N), a no feed treatment (NF), and a reference group of mussels from the Nottoway River (NR), Virginia. The levels of protein and glucose differed among treatments (p<0.0001), but glycogen and percentage tissue moisture did not (p>0.17). Production of ripe and developing gametes differed significantly among treatments (p=0.001), but stage of gamete development did not (p=0.70). Lipid levels and muscle fiber areas of treatment groups differed significantly (p<0.0001). Results of the feeding trial indicate that S. quadricauda is a suitable feed for E. complanata, but future experiments should identify algal species higher in carbohydrates for a mixed algal diet. To determine sex and stage of gametogenesis, tissue histological sections from gonads of Villosa iris and Utterbackia imbecillis were evaluated. Occurrences of oogenic, spermatogenic, and hermaphroditic tissues were summarized in frequency tables. Visceral sites from which similar tissues were collected from conspecific specimens were evaluated for gametogenic stage. Sex was accurately determined in the central, visceral portion V. iris and female regions of U. imbecillis; and spermatogenic tissue was consistent in the dorsal-anterior areas of U. imbecillis. These areas also provided accurate determination of gamete stage in specimens. Reproductive asynchrony was observed among males and females (p<0.02). Male regions of U. imbecillis showed gamete stage characterized by mature and developing spermatogenic tissue, while 2 groups of mussels were showed oogenic development characterized by mature oocytes and resorption of gametes. Male V. iris showed early gamete development without mature spermatozoa, and 2 groups of female V. iris showed mature and developing gametes and resorption of gametes. Protocols for biopsy tissue collection from selected visceral areas were developed for U. imbecillis and V. iris for sex determination and staging of gametogenesis. The application of this biopsy protocol should be considered population specific, and protocols appropriate for other populations and species should be developed with methods of this study. Advisors/Committee Members: Neves, Richard J. (committeechair), Saacke, Richard G. (committee member), Caceci, Thomas (committee member), Smith, Stephen A. (committee member), Lemly, A. Dennis (committee member).

Subjects/Keywords: freshwater mussels; hermaphroditism; diet; Unionidae; gametogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Henley, W. F. (2002). Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae). (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/27932

Chicago Manual of Style (16^th Edition):

Henley, William F. “Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae).” 2002. Doctoral Dissertation, Virginia Tech. Accessed March 08, 2021. http://hdl.handle.net/10919/27932.

MLA Handbook (7^th Edition):

Henley, William F. “Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae).” 2002. Web. 08 Mar 2021.

Vancouver:

Henley WF. Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae). [Internet] [Doctoral dissertation]. Virginia Tech; 2002. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10919/27932.

Council of Science Editors:

Henley WF. Evaluation of Diet, Gametogenesis, and Hermaphroditism in Freshwater Mussels (Bivalvia: Unionidae). [Doctoral Dissertation]. Virginia Tech; 2002. Available from: http://hdl.handle.net/10919/27932

University of Otago

26. Heenan, Samuel. Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand .

Degree: University of Otago

URL: http://hdl.handle.net/10523/9974

► The flat oyster, Ostrea chilensis, is an important cultural and commercial species in New Zealand fishing and aquaculture industries. However, natural populations and commercial operations… (more)

▼ The flat oyster, Ostrea chilensis, is an important cultural and commercial species in New Zealand fishing and aquaculture industries. However, natural populations and commercial operations are threatened by haplosporidian parasites in the genus Bonamia. Mass mortality from a Bonamia exitiosa infection epidemic during 1986-1992 led to a period of closure in New Zealand’s largest flat oyster fishery at Foveaux Strait, and recent infections by a new incursion of Bonamia ostreae have forced the destocking of oyster farms nationwide. Recruitment issues have also been identified as key drivers of these commercial populations, and there is a need to improve the understanding of the reproductive biology for O. chilensis. Furthermore, B. exitiosa proliferation is thought to be controlled by O. chilensis gametogenic cycle. With these key issues in mind, the present study aimed to improve the understanding of aspects of O. chilensis reproduction, patterns of B. exitiosa infection, and to compare gametogenesis processes with those observed in Foveaux Strait before the 1986 epizootic. The gametogenic cycle, biochemical composition, and the intensity of B. exitiosa infection in Foveaux Strait O. chilensis was examined monthly at one location from July 2017 to September 2018, with spatial variation being investigated seasonally from spring 2017 to spring 2018, across five sites. Monthly histological examination of O. chilensis gonad tissue revealed that the gametogenic cycle was relatively similar to that described before bonamiasis epidemics, with the onset of a continuous spawning event beginning in spring 2017. However, there was evidence for a more female skewed sex ratio, with less than 5% purely female oysters overall described previously, while almost one quarter of all samples were exclusively female in the present study. In addition, the development of female reproductive features at smaller sizes was observed, as well as an increased component of gonadal inactivity. Monthly examination of the total percentage protein, carbohydrate and lipid content of oysters revealed consistent seasonal patterns in the utilisation and accumulation of reserves. Carbohydrates were depleted over the winter and spring, while high concentrations persisted through summer and autumn. While proteins and lipids exhibited the opposite trend, and were accumulated through winter, peaking in spring, with extensive utilisation occurring over summer and autumn. Oysters in western Foveaux Strait may have exhibited more conservative elements in the utilisation and accumulation of biochemical components, relying more on reserves for spawning. While eastern populations were more opportunistic, relying less onbiochemical reserves during spawning, and conserving these constituents for utilisation during lean autumn and winter months. This may have been related to differences in B. exitiosa infection, as qPCR assays of a subset of samples suggested a higher prevalence in western Foveaux Strait, and biochemical reserves were lower in infected oysters.… Advisors/Committee Members: Lamare, Miles (advisor).

Subjects/Keywords: Oyster; Ostrea; chilensis; Bonamia; Gametogenesis; Biochemical

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heenan, S. (n.d.). Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand . (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/9974

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

Heenan, Samuel. “Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand .” Masters Thesis, University of Otago. Accessed March 08, 2021. http://hdl.handle.net/10523/9974.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

Heenan, Samuel. “Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand .” Web. 08 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Heenan S. Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand . [Internet] [Masters thesis]. University of Otago; [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10523/9974.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

Heenan S. Aspects of Reproduction in a Post-Bonamia exitiosa Epidemic Ostrea chilensis Fishery, Foveaux Strait, Southern New Zealand . [Masters Thesis]. University of Otago; Available from: http://hdl.handle.net/10523/9974

Note: this citation may be lacking information needed for this citation format:
No year of publication.

27. Lueg, Jenna R. Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida.

Degree: 2011, Nova Southeastern University

URL: http://nsuworks.nova.edu/occ_stuetd/202

► Restoration efforts are being implemented in many of the world’s coral reefs due to damages from anthropogenic sources such as ship groundings and anchor damage.… (more)

▼ Restoration efforts are being implemented in many of the world’s coral reefs due to damages from anthropogenic sources such as ship groundings and anchor damage. One restoration technique involves attempts to save dislodged and fragmented coral colonies by transplanting them back to damage sites. Research has shown that survivorship and growth of transplanted colonies is comparable to that of natural, control colonies. What remains unknown is to what extent transplantation affects the ecological success and reproduction of dislodged and fragmented coral colonies. The purpose of this study was twofold. Reproduction and spawning information is sparse for S. intersepta and Solenastrea bournoni, so the first purpose was to describe gamete development of these two species and assess correlations between environmental dynamics and spawning of each species. Tissue samples were collected throughout Broward County, Florida and processed for histological examination. Gametes were counted, and development was assessed. For S. intersepta and S. bournoni, late stage oocyte abundance was compared with environmental factors of mean daily water temperatures at depth, lunar phase, semidiurnal tides and solar insolation for correlative evidence to predict future spawning events. Findings indicated that both S. bournoni and S. intersepta are gonochoric broadcast spawners. Solenastrea bournoni spawns annually after the full moon in September when sea temperatures are at a maximum. Stephanocoenia intersepta spawns annually after the full moon of August or September, depending on the timing of the full moon. The second purpose was to determine if previously transplanted Porites astreoides, Montastraea cavernosa, Siderastrea siderea and Stephanocoenia intersepta corals produce gametes and spawn similarly to naturally occurring colonies and to address the issue of transplantation as a suitable resource management tool to aid in reef recovery for future coral generations. Results indicated no significant difference in fecundity between transplants and controls of M. cavernosa, S. siderea or S. intersepta. A significant difference was found in fecundity between P. astreoides transplants and controls, but it is thought that it is due to a difference in depth of collected samples. Overall, this study shows that transplantation of coral colonies after damage and fragmentation events does not have adverse effects on the long-term fecundity of coral colonies.

Subjects/Keywords: Solenastrea bournoni; Stephanocoenia intersepta; Porites astreoides; Montastrea cavernosa; Siderastrea siderea; gametogenesis; spawning; transplantation; Marine Biology; Oceanography and Atmospheric Sciences and Meteorology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lueg, J. R. (2011). Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida. (Thesis). Nova Southeastern University. Retrieved from http://nsuworks.nova.edu/occ_stuetd/202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lueg, Jenna R. “Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida.” 2011. Thesis, Nova Southeastern University. Accessed March 08, 2021. http://nsuworks.nova.edu/occ_stuetd/202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lueg, Jenna R. “Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida.” 2011. Web. 08 Mar 2021.

Vancouver:

Lueg JR. Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida. [Internet] [Thesis]. Nova Southeastern University; 2011. [cited 2021 Mar 08]. Available from: http://nsuworks.nova.edu/occ_stuetd/202.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lueg JR. Gametogenesis and Spawning of Solenastrea bournoni and Stephanocoenia intersepta and the Fecundity of Four Common Transplanted Coral Species Offshore, Southeast Florida. [Thesis]. Nova Southeastern University; 2011. Available from: http://nsuworks.nova.edu/occ_stuetd/202

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Anelise dos Santos Mendonça. Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese.

Degree: 2013, Federal University of Uberlândia

URL: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4818

►

DNA methylation is one of the most studied epigenetic events and is responsible for epigenetic reprogramming which occurs during gametogenesis. Understanding how this reprogramming occurs… (more)

▼

DNA methylation is one of the most studied epigenetic events and is responsible for epigenetic reprogramming which occurs during gametogenesis. Understanding how this reprogramming occurs in oogenesis is important to comprehend physiologic and genetic aspects involved in female gametogenesis in order to create parameters for oocyte competence and, consequently, to improve the in vitro embryo production, maximizing the use of gametes and improving production rates. The aim of this study was to evaluate the DNA methylation pattern in two DMRs involved in the control of XIST and IGF2 genes expression in oocytes from pre antral and antral follicles of Nellore cows. The extracted DNA from oocytes was treated with sodium bisulphite and amplified to XIST and IGF2 genes, which was cloned into DH5 cells, and then purified and sequenced. The methylation patterns found for oocytes of primordial, secondary, incompetent antral and competent antral follicles were 91.59 6.4%, 85.70 19.6%, 91.25 7.2% and 92.58 11.7%, respectively for XIST gene and 60.56 29.1%, 59.68 34.6%, 58.21 33.0% and 67.47 27.8%, respectively for IGF2 gene. XIST is more methylated than IGF2 gene (P<0,001). The hypermethylated pattern of XIST gene suggests that this event may be responsible for epigenetic reactivation of the X chromosome during oogenesis, which is observed in the final oocyte. The IGF2 gene was also hypermethylated, a different pattern found in matured oocytes. This suggests that the analyzed regions undergo differents epigenetic reprogramming processes during oogenenesis, which are only completed with oocyte maturation.

A metilação do DNA é um dos eventos epigenéticos mais conhecidos, sendo um dos responsáveis pela reprogramação epigenética que acontece durante a gametogênese. Entender como ocorre essa reprogramação na ovogênese é importante para a compreensão de aspectos fisiológicos e genéticos envolvidos na gametogênese feminina com o intuito de criar parâmetros para a competência ovocitária e, consequentemente, melhorar a produção in vitro de embriões, maximizando a utilização de gametas e melhorando as taxas de produção. Nesse trabalho objetivou-se determinar o padrão de metilação em duas DMRs envolvidas no controle da expressão dos genes XIST e IGF2 em ovócitos de folículos pré-antrais e antrais de vacas Nelore. O DNA extraído dos ovócitos foi tratado com bissulfito de sódio e amplificado para os genes XIST e IGF2, o qual foi clonado em células DH5, sendo em seguida purificado e sequenciado. Foram encontrados padrões de metilação para ovócitos de folículos primordiais, secundários finais, antrais incompetentes e antrais competentes de 91,59 6,4%, 85,70 19,6%, 91,25 7,2% e 92,58 11,7%, respectivamente para o gene XIST e 60,56 29,1%, 59,68 34,6%, 58,21 33,0% e 67,47 27,8%, respectivamente para o gene IGF2, sendo que o gene XIST está mais metilado que o gene IGF2 (P<0,001). O padrão hipermetilado para o gene XIST sugere que pode ser este o evento epigenético responsável pela reativação do cromossomo X durante a ovogênese, caráter que é…

Advisors/Committee Members: Ana Maria Bonetti, Ricarda Maria dos Santos, Maurício Machaim Franco, Margot Alves Nunes Dode.

Subjects/Keywords: Bovino; Gametogênese; IGF2; Reprogramação Epigenética; XIST; CLINICA VETERINARIA; Bovino - Melhoramento genético; Bovino - Reprodução; Bovine; DNA Methylation; Epigenetic Reprogramming; Gametogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mendonça, A. d. S. (2013). Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese. (Thesis). Federal University of Uberlândia. Retrieved from http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mendonça, Anelise dos Santos. “Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese.” 2013. Thesis, Federal University of Uberlândia. Accessed March 08, 2021. http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mendonça, Anelise dos Santos. “Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese.” 2013. Web. 08 Mar 2021.

Vancouver:

Mendonça AdS. Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese. [Internet] [Thesis]. Federal University of Uberlândia; 2013. [cited 2021 Mar 08]. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4818.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mendonça AdS. Padrão de metilação dos genes XIST e IGF2 em ovócitos de vacas nelore (Bos taurus indicus) em diferentes fases da ovogênese. [Thesis]. Federal University of Uberlândia; 2013. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=4818

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

29. De Villiers, Charon. The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro .

Degree: 2006, University of the Western Cape

URL: http://hdl.handle.net/11394/2266

► Gonadotropin Releasing Hormone (GnRH) is a hypothalmic decapeptide, which regulates mammalian gonadotropin secretions by binding to specific, high affinity receptors in the pituitary. Two forms… (more)

Subjects/Keywords: Gametogenesis; Spermatozoa; Motility; Monkeys as laboratory animals

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

De Villiers, C. (2006). The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/2266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

De Villiers, Charon. “The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro .” 2006. Thesis, University of the Western Cape. Accessed March 08, 2021. http://hdl.handle.net/11394/2266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

De Villiers, Charon. “The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro .” 2006. Web. 08 Mar 2021.

Vancouver:

De Villiers C. The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro . [Internet] [Thesis]. University of the Western Cape; 2006. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/11394/2266.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

De Villiers C. The effect of gonadotropin-releasing hormones (GnRH) I & II on sperm motility and acrosome status of the Vervet monkey (Chlorocebus aethiops) in vitro . [Thesis]. University of the Western Cape; 2006. Available from: http://hdl.handle.net/11394/2266

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Kuldeep, Lal Kumar. Studies on the reproductive physiology of lazes calcarifer (BLOCH).

Degree: 1991, Cochin University of Science and Technology

URL: http://dyuthi.cusat.ac.in/purl/3449

►

At present, there is paucity of information regarding the breeding biology, especially from southeastern region, which differs in its climatic features from northeastern coastal areas,… (more)

Subjects/Keywords: Gonadal cycle; Gametogenesis; Gonad maturation; Sexuality; Fecundity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuldeep, L. K. (1991). Studies on the reproductive physiology of lazes calcarifer (BLOCH). (Thesis). Cochin University of Science and Technology. Retrieved from http://dyuthi.cusat.ac.in/purl/3449

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuldeep, Lal Kumar. “Studies on the reproductive physiology of lazes calcarifer (BLOCH).” 1991. Thesis, Cochin University of Science and Technology. Accessed March 08, 2021. http://dyuthi.cusat.ac.in/purl/3449.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuldeep, Lal Kumar. “Studies on the reproductive physiology of lazes calcarifer (BLOCH).” 1991. Web. 08 Mar 2021.

Vancouver:

Kuldeep LK. Studies on the reproductive physiology of lazes calcarifer (BLOCH). [Internet] [Thesis]. Cochin University of Science and Technology; 1991. [cited 2021 Mar 08]. Available from: http://dyuthi.cusat.ac.in/purl/3449.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuldeep LK. Studies on the reproductive physiology of lazes calcarifer (BLOCH). [Thesis]. Cochin University of Science and Technology; 1991. Available from: http://dyuthi.cusat.ac.in/purl/3449

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations