subject:(fusion protein)

Addis Ababa University

1. LIKU, BEKELE. EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING .

Degree: 2008, Addis Ababa University

URL: http://etd.aau.edu.et/dspace/handle/123456789/553

► Cervical cancer is a serious public health problem of global importance. Nearly 80% of the half-a-million new cases reported annually occur in developing countries. The… (more)

▼ Cervical cancer is a serious public health problem of global importance. Nearly 80% of the half-a-million new cases reported annually occur in developing countries. The rate of cervical cancer in Ethiopia is estimated to be as high as 32% of all female malignancies diagnosed in hospitals. Over 90% of cervical cancer is attributable to certain HPV infections. HPV -16 and -18 have been recognized as the first and the second most common HPV genotypes in all regions, and were detected in 74% of all cases globally. The recognition that infection with HPV is essential for the development of cervical cancer has led to the development not only of HPV vaccines, but also of cervical cancer screening strategies that incorporate HPV testing. For Ethiopia to benefit from these developments, epidemiological surveys of HPV prevalence and knowledge of the immune response patterns to HPV proteins with diagnostic potential is fundamental. In this study, we investigated whether antibody response to selected oncoproteins of the two most frequent HPV types could serve as screening assay for invasive cervical cancer. We recruited 271 cervical cancer patients and 107 women with different non-cervical cancer disease conditions from the gynaecologic outpatient clinics of a tertiary level university hospital. We obtained fresh biopsy specimens from cervical cancer cases and blood from all the study participants. Antibodies against E6, E7 and L1 proteins of HPV-16 and -18 in plasma were determined using ELISA that uses glutathione-casein conjugate to capture recombinant protein antigens fused to glutathione S-trasnferase. HPV DNA detection and typing was done by L1 consensus PCR with PGMY09/011 biotinylated primers followed by hybridisation to a line blot. A strong association of E6 and/or E7 with cervical carcinoma was observed among Ethiopian cervical cancer patients as well, with 72.7% positives in the cases and 14.9% in the controls (OR=12.8,95% CI 6.2-26.4) (p<0.001). Antibodies against HPV-16 L1 were also associated with cervical cancer (OR=2.6, 95% CI 1.4-4.8). Antibody responses to E6 and E7 were dependent on the clinical stage of the disease, with 38.5% positives in FIGO Stage 0, 57.1% in Stage I and reaching to 83.5% in Stage III (p<0.05). There was no correlation between antibody response to HPV-16 E6 and E7 oncoproteins and antibodies against HPV-16 L1 capsid protein. The HPV serological assays were also compared as screening/diagnostic tests for invasive cervical cancer. The sensitivity estimates were 72% and 73% for E6 and/or E7 ELISA and E6, E7 and L1 ELISA in combination, respectively. x Specificity estimates were highest for E7 oncoprotein based ELISA (96.3%). A similar pattern was observed, with regard to the detection of the genotype of HPV present in the tumour. The HPV-16 E6, E7 and L1 ELISA combined were able to detect the type of HPV DNA in 30/32 patients with HPV-16 positive tumour. These data suggest that antibodies against E6, E7 and L1 protein are good candidates for use as markers of cervical HPV infections and the… Advisors/Committee Members: Dr. Yohannes Mengistu (advisor).

Subjects/Keywords: ELISA; GST fusion protein; HPV; Cervical cancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LIKU, B. (2008). EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING . (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

LIKU, BEKELE. “EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING .” 2008. Thesis, Addis Ababa University. Accessed April 18, 2021. http://etd.aau.edu.et/dspace/handle/123456789/553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

LIKU, BEKELE. “EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING .” 2008. Web. 18 Apr 2021.

Vancouver:

LIKU B. EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING . [Internet] [Thesis]. Addis Ababa University; 2008. [cited 2021 Apr 18]. Available from: http://etd.aau.edu.et/dspace/handle/123456789/553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

LIKU B. EVALUATION OF SEROLOGICAL RESPONSE TO ONCOPROTEINS OF HUMAN PAPILLOMAVIRUS TYPES 16 AND 18 AS POTENTIAL SEROMARKERS FOR CERVICAL CANCER SCREENING . [Thesis]. Addis Ababa University; 2008. Available from: http://etd.aau.edu.et/dspace/handle/123456789/553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Washington

2. Vulovic, Ivan. Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles.

Degree: PhD, 2020, University of Washington

URL: http://hdl.handle.net/1773/45809

► Innovation in the symmetric assembly and protein material design space has the potential to – eventually – reinvent medicine and nanotechnology. One leading strategy for… (more)

Subjects/Keywords: genetic fusion; protein design; Biochemistry; Molecular engineering

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APA (6^th Edition):

Vulovic, I. (2020). Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/45809

Chicago Manual of Style (16^th Edition):

Vulovic, Ivan. “Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles.” 2020. Doctoral Dissertation, University of Washington. Accessed April 18, 2021. http://hdl.handle.net/1773/45809.

MLA Handbook (7^th Edition):

Vulovic, Ivan. “Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles.” 2020. Web. 18 Apr 2021.

Vancouver:

Vulovic I. Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles. [Internet] [Doctoral dissertation]. University of Washington; 2020. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1773/45809.

Council of Science Editors:

Vulovic I. Software Algorithms for Design of Symmetric Protein Complexes Applied to Cryo-Electron Microscopy Scaffolds and Antibody Nanoparticles. [Doctoral Dissertation]. University of Washington; 2020. Available from: http://hdl.handle.net/1773/45809

University of Southern California

3. Chen, Xiaoying. Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics.

Degree: PhD, Pharmaceutical Sciences, 2011, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/637770/rec/3837

► Recombinant fusion proteins have become an important class of biomolecules since the invention of recombinant DNA technology. As an indispensable component of recombinant fusion proteins,… (more)

Subjects/Keywords: linker; fusion protein; transferrin; pharmacokinetics; pharmacodynamics

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APA (6^th Edition):

Chen, X. (2011). Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/637770/rec/3837

Chicago Manual of Style (16^th Edition):

Chen, Xiaoying. “Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics.” 2011. Doctoral Dissertation, University of Southern California. Accessed April 18, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/637770/rec/3837.

MLA Handbook (7^th Edition):

Chen, Xiaoying. “Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics.” 2011. Web. 18 Apr 2021.

Vancouver:

Chen X. Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics. [Internet] [Doctoral dissertation]. University of Southern California; 2011. [cited 2021 Apr 18]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/637770/rec/3837.

Council of Science Editors:

Chen X. Linkers in transferrin fusion proteins: effects on pharmacokinetics and pharmacodynamics. [Doctoral Dissertation]. University of Southern California; 2011. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/637770/rec/3837

University of Vienna

4. Kitzler, Christian Manuel. Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy.

Degree: 2019, University of Vienna

URL: http://othes.univie.ac.at/56339/

►

In dieser Arbeit wurde die Bindungsstelle zwischen BRCA1 und MAX-MAX mittels NMR untersucht. Weiters wurde ein großes intrinsisch ungeordnetes Fragment von BRCA1 durch Fusion von… (more)

Subjects/Keywords: 42.13 Molekularbiologie; Protein Interaktion / BRCA1 / MAX / IDP / NMR / Protein Fusion; Protein interaction / BRCA1 / MAX / IDP / NMR / protein fusion

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APA (6^th Edition):

Kitzler, C. M. (2019). Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy. (Thesis). University of Vienna. Retrieved from http://othes.univie.ac.at/56339/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kitzler, Christian Manuel. “Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy.” 2019. Thesis, University of Vienna. Accessed April 18, 2021. http://othes.univie.ac.at/56339/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kitzler, Christian Manuel. “Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy.” 2019. Web. 18 Apr 2021.

Vancouver:

Kitzler CM. Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy. [Internet] [Thesis]. University of Vienna; 2019. [cited 2021 Apr 18]. Available from: http://othes.univie.ac.at/56339/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kitzler CM. Investigating the binding-site and possible long-range effects of the interaction between BRCA1 and MAX-MAX using NMR spectroscopy. [Thesis]. University of Vienna; 2019. Available from: http://othes.univie.ac.at/56339/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Temple University

5. Cheong, Jae Eun. DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS.

Degree: PhD, 2016, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,406966

►

Chemistry

This research presents a new approach to creating large, complex molecules to carry out molecular recognition and catalytic functions mimicking biological proteins. Development of… (more)

▼

Chemistry

This research presents a new approach to creating large, complex molecules to carry out molecular recognition and catalytic functions mimicking biological proteins. Development of new therapeutics that bind protein surfaces and disrupt protein-protein interactions was first addressed targeting the envelope transmembrane protein in HIV-1, gp41. In this work, spiroligomer inhibitors of gp41 were designed and synthesized, and then the biochemical activity was tested. Rationally designed inhibitors were developed using computational modeling with the Molecular Operating Environment software (MOE). To build the desired molecular shape according to the design, C-2 alkylation of a bis-amino acid monomer was investigated to synthesize the higher degree of bis-amino acids with various reaction conditions for access to all possible diastereomers. Based on this design and synthetic methodology, a spiroligomer targeting gp41 was built by synthesizing each monomer and then linking them together by diketopiperazine (DKP). For the biological evaluation, the gp41-5 gene was transformed into E. coli and the protein was expressed, purified, and refolded for an in vitro binding test. A direct binding, fluorescence polarization assay was used to evaluate the binding affinity of the functionalized spiroligomer to the gp41-5 protein. Its antiviral activity was assessed in collaboration with the Chaiken lab at Drexel University. In addition, investigation into how the unique structures provided by the spiroligomer backbone allow for various uses, such as functionalized struts in porous organic polymers (POPs). In the large internal space of a POP, a nucleophilic, catalytic spiroligomer was installed to increase the reaction rate for the hydrolysis of methyl paraoxon (a neurotoxin G agent stimulant). Spiroligomers were designed and synthesized with backbone DMAP moieties, and the activity of these catalysts was analyzed in collaboration with the Hupp lab at Northwestern University.

Temple University – Theses

Advisors/Committee Members: Schafmeister, Christian;, Andrade, Rodrigo B., Valentine, Ann M., Chaiken, Irwin M.;.

Subjects/Keywords: Chemistry;

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APA (6^th Edition):

Cheong, J. E. (2016). DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,406966

Chicago Manual of Style (16^th Edition):

Cheong, Jae Eun. “DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS.” 2016. Doctoral Dissertation, Temple University. Accessed April 18, 2021. http://digital.library.temple.edu/u?/p245801coll10,406966.

MLA Handbook (7^th Edition):

Cheong, Jae Eun. “DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS.” 2016. Web. 18 Apr 2021.

Vancouver:

Cheong JE. DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS. [Internet] [Doctoral dissertation]. Temple University; 2016. [cited 2021 Apr 18]. Available from: http://digital.library.temple.edu/u?/p245801coll10,406966.

Council of Science Editors:

Cheong JE. DEVELOPMENT OF SPIROLIGOMER SCAFFOLDS FOR INHIBITING HIV FUSION AND POROUS ORGANIC POLYMERS. [Doctoral Dissertation]. Temple University; 2016. Available from: http://digital.library.temple.edu/u?/p245801coll10,406966

University of the Western Cape

6. Choi, Yook-Wah. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .

Degree: 2011, University of the Western Cape

URL: http://hdl.handle.net/11394/5299

► Hepatitis C was first recognized as a transfusion-associated liver disease not caused by hepatitis A or hepatitis B virus after serological tests were developed to… (more)

▼ Hepatitis C was first recognized as a transfusion-associated liver disease not caused by hepatitis A or hepatitis B virus after serological tests were developed to screen for their presence in the blood. The infectious agent was finally identified with the cloning of the cDNA of hepatitis C virus (HCV) using random polymerase chain reaction (PCR) screening of nucleic acids extracted from plasma of a large pool of chimpanzee infected with non-A non-B hepatitis. NS5B, a membrane-associated RNA-dependent RNA polymerase essential in the replication of HCV, initiates the synthesis of a complementary negative-strand RNA from the genomic positive-strand RNA so that more positive-strand HCV RNA can then be generated from the newly synthesised negative-strand template. The crystal structure of NS5B presented typical fingers, palm and thumb sub-domains encircling the GDD active site, which is also seen in other RNA-dependent RNA polymerases, and is similar to the structure of reverse transcriptase of HIV-1 and murine Moloney leukaemia virus. The last 21 amino acids in the C-terminus of NS5B anchor the protein to the endoplasmic reticulum (ER)-derived membranous web. NS5B has been shown to interact with the core, NS3/NS4A, NS4B and NS5A proteins, either directly or indirectly. Numerous interactions with cellular proteins have also been reported. These proteins are mainly associated with genome replication, vesicular transport, protein kinase C-related kinase 2, P68 (DDX5), α-actinin, nucleolin, human eukaryotic initiation factor 4AII, and human VAMP-associated protein. Previous studies have confirmed that NS5B binds to full-length DDX5. By constructing deletion mutants of DDX5, we proceeded to characterize this interaction between DDX5 and HCV NS5B. We report here the identification of two exclusive HCV NS5B binding sites in DDX5, one in the N-terminal region of amino acids 1 to 384 and the other in the C-terminal region of amino acids 387 to 614. Proteins spanning different regions of DDX5 were expressed and purified for crystallization trials. The N-terminal region of DDX5 from amino acids 1 to 305 which contains the conserved domain I of the DEAD-box helicase was also cloned and expressed in Escherichia coli. The cloning, expression, purification and crystallization conditions are presented in this work. Subsequently, the crystal structure of DDX5 1-305 was solved and the high resolution three-dimensional structure shows that in front of domain I is the highly variable and disordered N terminal region (NTR) of which amino acids 51-78 is observable, but whose function is unknown. This region forms an extensive loop and supplements the core with an additional α-helix. Co-immunoprecipitation experiments demonstrated that the NTR of DDX5 1-305 auto-inhibit its interaction with NS5B. Interestingly, the α-helix in NTR is essential for this auto-inhibition and seems to mediate the interaction between the highly flexible 1-60 residues in NTR and NS5B binding site in DDX5 1-305, presumably located within residues 79-305.… Advisors/Committee Members: Fielding, B.C (advisor), Tan, YJ (advisor).

Subjects/Keywords: Hepatitis C virus (HCV); GST fusion protein; NS5B; Protein crystallisation screens

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APA (6^th Edition):

Choi, Y. (2011). Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Choi, Yook-Wah. “Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .” 2011. Thesis, University of the Western Cape. Accessed April 18, 2021. http://hdl.handle.net/11394/5299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Choi, Yook-Wah. “Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus .” 2011. Web. 18 Apr 2021.

Vancouver:

Choi Y. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . [Internet] [Thesis]. University of the Western Cape; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/11394/5299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Choi Y. Structural and functional characterization of human DDX5 and its interaction with NS5B of hepatitis C virus . [Thesis]. University of the Western Cape; 2011. Available from: http://hdl.handle.net/11394/5299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Southern California

7. Chen, Yu-Sheng. Production and characterization of proinsulin-transferrin fusion protein.

Degree: MS, Pharmaceutical Sciences, 2011, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/627825/rec/5258

► A transferrin-based fusion protein, proinsulin-transferrin (ProIns-Tf) had been constructed using recombinant protein technology in our laboratory. Based on the in vitro preliminary results, ProIns-Tf reduced… (more)

▼ A transferrin-based fusion protein, proinsulin-transferrin (ProIns-Tf) had been constructed using recombinant protein technology in our laboratory. Based on the in vitro preliminary results, ProIns-Tf reduced glucose production in H4IIE liver cells and increased glucose uptake in adipocytes. These potent effects were achieved by conversion of the ProIns moiety to an active Ins-like moiety through the mechanism of TfR-mediated endocytosis. Therefore, ProIns-Tf showed great potential for diabetes therapeutics. However, it was also observed that the preparation of this fusion protein also produced a large amount of protein impurities which might have impeding effects on the further characterization studies. ❧ In this report, ProIns-Tf fusion protein was purified using the polyhistidine tag technique. The hexahistidine tag (6xHis tag) was bound to the C-terminus of Tf domain through mutagenic polymerase chain reaction (PCR) and recombinant DNA technology. After the protein expression and purification processes, the protein analysis results indicated that the purity of ProIns-Tf was significantly enhanced. In addition, through the transferrin receptor (TfR) competitive binding assay, we found that the insertion of C-terminal 6xHis tag did not interfere with the binding affinity of Tf domain to TfR. Therefore, it was concluded that the His-tag purification is a suitable approach to improve the purity of Tf-based fusion protein. ❧ Furthermore, by comparing the results to previous studies in our laboratory, we also noticed that the TfR binding affinity of our Tf-based fusion proteins - ProIns-Tf, granulocyte colony-stimulating factor- Tf (G-CSF-Tf) and human growth hormone-Tf (hGH-Tf) - had a positive correlation to the molecular weight of the bound protein domain. It also implies that the beneficial effects of the TfR-mediated endocytosis should be limited by the size of Tf-bound protein drug. As a result, molecular weight of protein drug will be considered as an important criterion for the design of future Tf-based recombinant fusion proteins. Advisors/Committee Members: Shen, Wei-Chiang (Committee Chair), Okamoto, Curtis Toshio (Committee Member), Wang, Clay C. C. (Committee Member).

Subjects/Keywords: bifunctional fusion protein; his-tag; insulin; proinsulin; protein purification; transferrin

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APA (6^th Edition):

Chen, Y. (2011). Production and characterization of proinsulin-transferrin fusion protein. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/627825/rec/5258

Chicago Manual of Style (16^th Edition):

Chen, Yu-Sheng. “Production and characterization of proinsulin-transferrin fusion protein.” 2011. Masters Thesis, University of Southern California. Accessed April 18, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/627825/rec/5258.

MLA Handbook (7^th Edition):

Chen, Yu-Sheng. “Production and characterization of proinsulin-transferrin fusion protein.” 2011. Web. 18 Apr 2021.

Vancouver:

Chen Y. Production and characterization of proinsulin-transferrin fusion protein. [Internet] [Masters thesis]. University of Southern California; 2011. [cited 2021 Apr 18]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/627825/rec/5258.

Council of Science Editors:

Chen Y. Production and characterization of proinsulin-transferrin fusion protein. [Masters Thesis]. University of Southern California; 2011. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/627825/rec/5258

IUPUI

8. Ettaki, Zacharia Nabil. Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance.

Degree: 2020, IUPUI

URL: http://hdl.handle.net/1805/24500

►

Indiana University-Purdue University Indianapolis (IUPUI)

Animals depend on inheriting non-genetic information early in life to grow and develop naturally. This inherited, non-genetic information was previously… (more)

Subjects/Keywords: RNA; Protein; Halo; SNAP; Fusion Protein; Inheritance; RNA-binding protein; crystal; structure; antibody

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APA (6^th Edition):

Ettaki, Z. N. (2020). Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/24500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ettaki, Zacharia Nabil. “Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance.” 2020. Thesis, IUPUI. Accessed April 18, 2021. http://hdl.handle.net/1805/24500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ettaki, Zacharia Nabil. “Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance.” 2020. Web. 18 Apr 2021.

Vancouver:

Ettaki ZN. Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance. [Internet] [Thesis]. IUPUI; 2020. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1805/24500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ettaki ZN. Developing Novel Methods to Identify RNA-Associated Mechanisms for Inheritance. [Thesis]. IUPUI; 2020. Available from: http://hdl.handle.net/1805/24500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Peláez, Miguel Ángel Postigo. Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane.

Degree: Bioscience, 2019, University of Skövde

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573

► Inflammasomes are a group of protein complex that regulate inflammation throughcomplex signal transduction, although their specific mechanisms and structures have notbeen fully described. As… (more)

Subjects/Keywords: NLRP3; Inflammation; Fusion gene; PCR; Fusion protein; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi

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APA (6^th Edition):

Peláez, M. �. P. (2019). Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane. (Thesis). University of Skövde. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Peláez, Miguel Ángel Postigo. “Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane.” 2019. Thesis, University of Skövde. Accessed April 18, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Peláez, Miguel Ángel Postigo. “Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane.” 2019. Web. 18 Apr 2021.

Vancouver:

Peláez M�P. Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane. [Internet] [Thesis]. University of Skövde; 2019. [cited 2021 Apr 18]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Peláez M�P. Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane. [Thesis]. University of Skövde; 2019. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17573

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University

10. Desai, Tanvi. Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin.

Degree: PhD, Natural Sciences, 2014, Rice University

URL: http://hdl.handle.net/1911/76430

► The endoplasmic Reticulum (ER) is an important site for lipid synthesis, protein synthesis and transport. ER fusion is an essential process for its maintenance and… (more)

▼ The endoplasmic Reticulum (ER) is an important site for lipid synthesis, protein synthesis and transport. ER fusion is an essential process for its maintenance and biogenesis. Mutations in genes involved in this process cause Hereditary Spastic Paraplegia (HSP). These mutations are shown to affect intracellular trafficking and localization of membrane compartment. One of the important proteins causing early onset of HSP is Atlastin. Previous work in McNew lab at Rice University (Moss et al., 2011b) has shown that atlastin is involved in the homotypic fusion of the ER and the C-terminal cytoplasmic region of atlastin is essential for atlastin mediated fusion. During my studies presented in this thesis, I was able to demonstrate that the C-terminal cytoplasmic region of atlastin destabilizes lipid bilayers to facilitate fusion. The requirement of C-terminal cytoplasmic region is minimal when fusing two fluid (or unstable) lipid bilayers. The C-terminal cytoplasmic region of atlastin forms an amphipathic helix and mutations on the hydrophobic phase of the helix reduce fusion. These mutations are not dominant, as presence of full length atlastin on even one of the fusing lipid bilayers can significantly improve fusion during a heterotypic fusion reaction. Additionally, domain swaps between human atlastin-1 and drosophila atlastin show that the role of C-terminal cytoplasmic region is highly conserved. Also, during my research presented here in, I found that when the transmembrane region and C-terminal cytoplasmic region of human atlastin-1 were swapped with drosophila atlastin, it showed functional similarity. These results show that although atlastins in organisms play an important role in the ER fusion, there are likely species specific differences in how this is achieved. An understanding of atlastin mediated fusion should help in unraveling mechanisms of HSP pathogenesis and other disorders arising from dysfunctional ER. Advisors/Committee Members: McNew, James A. (advisor), Huang, Huey W. (committee member), Braam, Janet (committee member), Stern, Michael (committee member), Lwigale, Peter Yunju (committee member).

Subjects/Keywords: Membrane fusion; Membrane protein; Atlastin; Endoplasmic reticulum; Hereditary spastic paraplegia; Intracellular fusion; Axonal neuropathy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Desai, T. (2014). Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/76430

Chicago Manual of Style (16^th Edition):

Desai, Tanvi. “Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin.” 2014. Doctoral Dissertation, Rice University. Accessed April 18, 2021. http://hdl.handle.net/1911/76430.

MLA Handbook (7^th Edition):

Desai, Tanvi. “Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin.” 2014. Web. 18 Apr 2021.

Vancouver:

Desai T. Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin. [Internet] [Doctoral dissertation]. Rice University; 2014. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1911/76430.

Council of Science Editors:

Desai T. Characterization of Structure and Function Relationship between Domains of the ER Membrane Protein Atlastin. [Doctoral Dissertation]. Rice University; 2014. Available from: http://hdl.handle.net/1911/76430

North Carolina State University

11. Choi, Ucheor B. Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies.

Degree: PhD, Physics, 2010, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/6220

► Conformational information about proteins can often reveal the mechanisms of their biological functions. This thesis examines conformational aspects of the synaptic SNARE (soluble N-ethylmaleimide-sensitive factor… (more)

Subjects/Keywords: protein-protein interactions; synaptic vesicle; single molecule FRET; neurotransmitter release; membrane fusion

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APA (6^th Edition):

Choi, U. B. (2010). Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/6220

Chicago Manual of Style (16^th Edition):

Choi, Ucheor B. “Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies.” 2010. Doctoral Dissertation, North Carolina State University. Accessed April 18, 2021. http://www.lib.ncsu.edu/resolver/1840.16/6220.

MLA Handbook (7^th Edition):

Choi, Ucheor B. “Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies.” 2010. Web. 18 Apr 2021.

Vancouver:

Choi UB. Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies. [Internet] [Doctoral dissertation]. North Carolina State University; 2010. [cited 2021 Apr 18]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/6220.

Council of Science Editors:

Choi UB. Single molecule fluorescence reveals dynamic structures of SNARE protein assemblies. [Doctoral Dissertation]. North Carolina State University; 2010. Available from: http://www.lib.ncsu.edu/resolver/1840.16/6220

12. Tilbury, Maura A. The expression of recombinant proteins with biomedical importance in Escherichia coli.

Degree: 2020, NUI Galway

URL: http://hdl.handle.net/10379/16104

► Naturally-occurring peptides and proteins with diverse biological roles have enormous potential for use as therapeutics and biomaterials. While proteins of biomedical interest were traditionally isolated… (more)

▼ Naturally-occurring peptides and proteins with diverse biological roles have enormous potential for use as therapeutics and biomaterials. While proteins of biomedical interest were traditionally isolated from their native sources, advances in recombinant protein expression have now enabled safe, efficient and large-scale production of highly pure protein. This project utilised Escherichia coli as an expression host for two recombinant proteins with potential biomedical application: (i) a barnacle cement protein for investigation as a biomimetic glue, and (ii) human superoxide dismutase enzyme for targeted pulmonary delivery. Proteins involved in wet adhesion of marine organisms are of growing biomedical interest due to their potential use as surgical adhesives. Recombinant protein technology allows such adhesive proteins to be produced at the scale needed to investigate their adhesive mechanisms. The 19 kDa cement protein (cp19k) of the stalked barnacle Pollicipes pollicipes was expressed in E. coli BL21. Co-overproduction of E. coli molecular chaperones GroEL-GroES and trigger factor (TF) resulted in increased yields of soluble cp19k protein. Surface coat analysis revealed high adsorption of a cp19k-TF complex on both hydrophobic and hydrophilic surfaces but low adsorption of the recombinant cp19k (rPpolcp19k) protein. The purified recombinant protein also exhibited negligible adsorption on hydrophobic, neutral hydrophilic or charged self-assembled monolayers in surface plasmon resonance assays designed to mimic the barnacle cement gland and seawater conditions. Due to its low adhesive capability, the potential cohesive ability of the protein was investigated, using an amyloid-specific fluorometric assay, revealing self-assembly of the protein into amyloid fibrils under cement gland-like but not seawater conditions. Transmission electron microscopy and atomic force microscopy confirmed and further characterised the rPpolcp19k fibrils. Our findings that rPpolcp19k self-assembles into amyloid fibrils suggest a cohesive role for the protein which could be exploited in biomedical adhesion. Acute respiratory distress syndrome (ARDS) is a life-threatening respiratory failure syndrome that is characterised by increased permeability of the alveolar-capillary membrane, pulmonary oedema and the acute onset of hypoxemia. In healthy individuals, an oxidant-antioxidant equilibrium is maintained by antioxidants such as superoxide dismutase enzymes. During the acute phase of ARDS, however, neutrophil infiltration into the alveolar space results in x uncontrolled release of reactive oxygen species and proteases, overwhelming the antioxidant defences and leading to alveolar epithelial and lung endothelial injury. Several fusion proteins incorporating human Cu-Zn-superoxide dismutase protein (hSOD1) were designed for aerosol delivery by nebulisation. Expression of the hSOD1 fusion proteins in E. coli BL21 was optimised using co-overproduction of GroEL-GroES chaperones. The fusion proteins exhibited high superoxide dismutase activity… Advisors/Committee Members: Wall, Gerard, Science Foundation Ireland, European Regional Development Fund.

Subjects/Keywords: Escherichia coli; recombinant protein; barnacle; adhesion; functional amyloid; fusion protein; superoxide dismutase; Microbiology; Natural Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tilbury, M. A. (2020). The expression of recombinant proteins with biomedical importance in Escherichia coli. (Thesis). NUI Galway. Retrieved from http://hdl.handle.net/10379/16104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tilbury, Maura A. “The expression of recombinant proteins with biomedical importance in Escherichia coli.” 2020. Thesis, NUI Galway. Accessed April 18, 2021. http://hdl.handle.net/10379/16104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tilbury, Maura A. “The expression of recombinant proteins with biomedical importance in Escherichia coli.” 2020. Web. 18 Apr 2021.

Vancouver:

Tilbury MA. The expression of recombinant proteins with biomedical importance in Escherichia coli. [Internet] [Thesis]. NUI Galway; 2020. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10379/16104.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tilbury MA. The expression of recombinant proteins with biomedical importance in Escherichia coli. [Thesis]. NUI Galway; 2020. Available from: http://hdl.handle.net/10379/16104

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Chicago

13. Klosterman, Susan M. Control of Synaptic Transmission Through SNARE Complex Regulation.

Degree: 2013, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/10043

► Synaptic transmission requires the assembly of a highly conserved complex composed of the SNARE proteins Syntaxin, SNAP-25, and Synaptobrevin. The Syntaxin binding protein Tomosyn has… (more)

Subjects/Keywords: Synaptic transmission; Soluble N-ethylmaleimide sensitive fusion protein (NSF) Attachment Protein Receptor (SNARES)

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APA (6^th Edition):

Klosterman, S. M. (2013). Control of Synaptic Transmission Through SNARE Complex Regulation. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/10043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Klosterman, Susan M. “Control of Synaptic Transmission Through SNARE Complex Regulation.” 2013. Thesis, University of Illinois – Chicago. Accessed April 18, 2021. http://hdl.handle.net/10027/10043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Klosterman, Susan M. “Control of Synaptic Transmission Through SNARE Complex Regulation.” 2013. Web. 18 Apr 2021.

Vancouver:

Klosterman SM. Control of Synaptic Transmission Through SNARE Complex Regulation. [Internet] [Thesis]. University of Illinois – Chicago; 2013. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10027/10043.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Klosterman SM. Control of Synaptic Transmission Through SNARE Complex Regulation. [Thesis]. University of Illinois – Chicago; 2013. Available from: http://hdl.handle.net/10027/10043

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cincinnati

14. Gow, Chien-Hung, M.D. Novel mechanisms of transcriptional regulation by leukemia fusion proteins.

Degree: PhD, Medicine: Cancer and Cell Biology, 2014, University of Cincinnati

URL: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394724980

► Transcription factors and chromatin structure are master regulators of homeostasis during hematopoiesis. Regulatory genes for each stage of hematopoiesis are activated or silenced in a… (more)

▼ Transcription factors and chromatin structure are master regulators of homeostasis during hematopoiesis. Regulatory genes for each stage of hematopoiesis are activated or silenced in a precise, finely tuned manner. Many leukemia fusion proteins are produced by chromosomal translocations that interrupt important transcription factors and disrupt these regulatory processes. Leukemia fusion proteins E2A-Pbx1 and AML1-ETO involve normal function transcription factor E2A, resulting in two distinct types of leukemia: E2A-Pbx1 t(1;19) acute lymphoblastic leukemia (ALL) and AML1-ETO t(8;21) acute myeloid leukemia (AML). E2A, a member of the E-protein family of transcription factors, is a key regulator in hematopoiesis that recruits coactivators or corepressors in a mutually exclusive fashion to regulate its direct target genes. In t(1;19) ALL, the E2A portion of E2A-Pbx1 mediates a robust transcriptional activation; however, the transcriptional activity of wild-type E2A is silenced by high levels of corepressors, such as the AML1-ETO fusion protein in t(8;21) AML and ETO-2 in hematopoietic cells. It is unclear how this context-dependent regulation of E2A-dependent transcription allows E2A protein to regulate transcription in response to variable intracellular levels of corepressor. In this study, we discovered that unlike HEB, another E-protein, the activation domain 1 (AD1) of E2A is inhibited for corepressor interaction by E2A-specific amino acid changes in the p300/CBP and ETO target motif, which interacts with only one of these targets at a time. At physiological levels of corepressor, E2A-Pbx1 escapes endogenous ETO-2 binding to confer its oncogenic ability via AD1 and AD2 cooperative activity to facilitate coactivator recruitment. In the presence of aberrantly high levels of AML1-ETO, E2A interacts with the corepressor to silence expression of E-protein target genes. This process requires the downstream ETO-interacting sequence (DES) domain of E2A to compensate for the weak binding between E2A-AD1 and AML1-ETO. This novel E2A-specific cofactor exchange mechanism provides an explanation for the specific E2A determinants that oppositely link corepressor function to distinct leukemogenic pathways. In the second part of this study, we explored the regulatory mechanism of histone deacetylase 3 (HDAC3), which mediates chromatin structural dynamics to control transcription. In E-protein-mediated transcription, corepressor AML1-ETO/ETO associates with HDAC3 by recruiting nuclear receptor corepressors N-CoR/SMRT to packed chromatin, thus repressing transcription. Functional HDAC3 must form a stable complex with N-CoR or SMRT, which individually and independently bind to HDAC3. We identified a novel cell-independent HDAC3 regulatory mechanism, which is significant for its reduced protein levels of corepressor (N-CoR or SMRT) and accelerates HDAC3 clearance to prevent complex formation between HDAC3 and its other corepressor. We also demonstrated the formation of an HDAC3-corepressor complex via a stepwise procedure in which the… Advisors/Committee Members: Khan, Sohaib (Committee Chair).

Subjects/Keywords: Oncology; transcriptional regulation; leukemia fusion protein; E-protein; E2A-Pbx1; AML1-ETO; HDAC3

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gow, Chien-Hung, M. D. (2014). Novel mechanisms of transcriptional regulation by leukemia fusion proteins. (Doctoral Dissertation). University of Cincinnati. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394724980

Chicago Manual of Style (16^th Edition):

Gow, Chien-Hung, M D. “Novel mechanisms of transcriptional regulation by leukemia fusion proteins.” 2014. Doctoral Dissertation, University of Cincinnati. Accessed April 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394724980.

MLA Handbook (7^th Edition):

Gow, Chien-Hung, M D. “Novel mechanisms of transcriptional regulation by leukemia fusion proteins.” 2014. Web. 18 Apr 2021.

Vancouver:

Gow, Chien-Hung MD. Novel mechanisms of transcriptional regulation by leukemia fusion proteins. [Internet] [Doctoral dissertation]. University of Cincinnati; 2014. [cited 2021 Apr 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394724980.

Council of Science Editors:

Gow, Chien-Hung MD. Novel mechanisms of transcriptional regulation by leukemia fusion proteins. [Doctoral Dissertation]. University of Cincinnati; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394724980

NSYSU

15. Chan, Yu-Lin. Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î².

Degree: Master, Biological Sciences, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1113112-160654

► Grouper (Epinephelus coioides) is one of the important farmed fish in the southern Taiwan. However, grouper aquaculture in Taiwan has a serious problem of infection,… (more)

Subjects/Keywords: antiserum; lipopolysarcharide; fusion protein; IL -1Î²; Epinephelus coioides

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APA (6^th Edition):

Chan, Y. (2012). Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î². (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1113112-160654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chan, Yu-Lin. “Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î².” 2012. Thesis, NSYSU. Accessed April 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1113112-160654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chan, Yu-Lin. “Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î².” 2012. Web. 18 Apr 2021.

Vancouver:

Chan Y. Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î². [Internet] [Thesis]. NSYSU; 2012. [cited 2021 Apr 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1113112-160654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chan Y. Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-Production and characterization of polyclonal antibody against Epinephelus coioides interleukin-1Î². [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-1113112-160654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

16. Chang, Shuo-Fu. Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I.

Degree: Master, Biological Sciences, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0327113-142030

► Orange-spotted Grouper (Epinephelus coioides) is one of the important economically farmed fish in Taiwan. However, grouper aquaculture in Taiwan has a serious problem of infection,… (more)

▼ Orange-spotted Grouper (Epinephelus coioides) is one of the important economically farmed fish in Taiwan. However, grouper aquaculture in Taiwan has a serious problem of infection, especially in grouper larvae breeding stage. The infection resulted in very high mortality, which causes massive economic loss. Therefore, early detection of pathogen is critical to prevent epidemic outbreak. Retinoic acid inducible gene-I (RIG-I) is an intracellular receptor recognizing dsRNA virus. Upon dsRNA virus infection, RIG-I is induced and over-expressed in protein level that mediating type I Interferon (IFNs) antiviral signaling. In this study, total RNA extracted from fertilized eggs of Epinephelus coioides was extracted for the reverse transcription-polymerase chain reaction (RT-PCR) amplification of 432 bp cDNA encoding regulatory domain (RD) of RIG-I protein. The amplified cDNA fragment was then cloned into pQE32 for the prokaryotic expression of 17 kDa His-tagged RIG-I (His-RIG-I-RD) fusion protein. His-RIG-I-RD fusion protein was purified for immunization of New Zealand white rabbit to produce antiserum against RIG-I protein. Our Western blot result confirmed the specificity of antiserum for recognizing 0.01 Î¼g His-RIG-I-RD fusion protein at 1:5000 dilution. In addition, 0.02 Î¼g of immunogen His-RIG-I-RD fusion protein can be readily detected at 1:25000 dilution. Furthermore, this antiserum could detect the increase of a 72 kDa protein in lysate prepared from head kidney of dsRNA viral mimic poly(I:C) injected Epinephelus coioides, but not in uninjected control fish. The immunostaining results of frozen head kidney sections also revealed the RIG-I immunoreactivity using this antiserum. RIG-I protein immunoreactivity was prominently increased in melanomacrophage center (MMC) of head kidney from poly(I:C) injected fish compared to that of uninjected control fish. In summary, we have generated a specific antiserum against RIG-I protein of Epinephelus coioides, which could be a useful reagent for further study of Grouper immune response and signal transduction upon virus infection. Advisors/Committee Members: Tsung-Chou Chang (chair), Jiin-Tsuey Cheng (committee member), Chung-Da Yang (chair).

Subjects/Keywords: Epinephelus coioides; RIG-I; poly(I:C); antiserum; fusion protein

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APA (6^th Edition):

Chang, S. (2013). Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0327113-142030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Shuo-Fu. “Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I.” 2013. Thesis, NSYSU. Accessed April 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0327113-142030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Shuo-Fu. “Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I.” 2013. Web. 18 Apr 2021.

Vancouver:

Chang S. Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Apr 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0327113-142030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang S. Production and characterization of polyclonal antibody against Epinephelus coioides retinoic acid inducible gene-I. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0327113-142030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. 小寺, 隆三. Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model.

Degree: 博士(医学), 2016, Oita University / 大分大学

URL: http://hdl.handle.net/10559/15609

► Bone fusion involves a complex set of regulated signaling pathways that control the formation of new bone matrix and the resorption of damaged bonematrix at… (more)

▼ Bone fusion involves a complex set of regulated signaling pathways that control the formation of new bone matrix and the resorption of damaged bonematrix at the surgical site. It has been reported that systemically administering a single dose of zoledronic acid (ZA) at the optimal time increases the strength of the bone morphogenetic protein (BMP)–mediated callus. In the present study,we aimed to investigate the effect of BMP-2 and ZA in a rat spinal model. Sixty-seven ratswere divided into 6 groups: group I (n=11) animalswere implantedwith a carrier alone, group II (n=12) animals were implanted with a carrier and a subcutaneous injection of ZA was administered 2weeks after surgery, group III (n=12) animals were implanted with a carrier containing 1 μg of rhBMP-2, group IV (n=12) animals were implanted with a carrier containing 1μg of rhBMP-2 and a subcutaneous injection of ZA was administered 2weeks after surgery, group V (n=10) animalswere implantedwith a carrier containing 3 μg of rhBMP-2, and group VI (n=10) animals were implanted with a carrier containing 3 μg of rhBMP-2 and a subcutaneous injection of ZA was administered 2weeks after surgery. The rats were euthanized after 6weeks, and their spines were explanted and assessed by manual palpation, radiography, high-resolution micro-computerized tomography (micro-CT), and histologic analysis. The fusion rates in group VI (60%) were considerably higher than those in the groups I (0%), II (0%), III (12.5%), IV (20.8%), and V (35%), (P b 0.05). Additionally, the radiographic scores of group VI were higher than those in the other groups, (P b 0.05). In micro-CT analysis, the tissue and bone volumes of the callus were significantly higher in group VI than those in the other groups, (P b 0.05). The trabecular number was significantly higher and the trabecular spacing was significantly lower in group VI than those in the other groups, (P b 0.05). The combination of rhBMP-2 and ZA administered systemically as a single dose at the optimal timewas efficacious in our rat spinal fusionmodel. Our results suggest that this combination facilitates spinal fusion and has potential clinical application.

Subjects/Keywords: Bone morphogenetic protein; Zoledronic acid; Spine fusion; Rat model

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

小寺, �. (2016). Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model. (Thesis). Oita University / 大分大学. Retrieved from http://hdl.handle.net/10559/15609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

小寺, 隆三. “Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model.” 2016. Thesis, Oita University / 大分大学. Accessed April 18, 2021. http://hdl.handle.net/10559/15609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

小寺, 隆三. “Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model.” 2016. Web. 18 Apr 2021.

Vancouver:

小寺 �. Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model. [Internet] [Thesis]. Oita University / 大分大学; 2016. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10559/15609.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

小寺 �. Manipulation of anabolic and catabolic responses with bone morphogenetic protein and zoledronic acid in a rat spinal fusion model. [Thesis]. Oita University / 大分大学; 2016. Available from: http://hdl.handle.net/10559/15609

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

18. Nelson, Katelyn. Functional Characterization of Oncogenic Driver FGFR3-TACC3.

Degree: Chemistry, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/9tj8f2k3

► Fibroblast Growth Factor Receptors (FGFRs) are critical for cell proliferation and differentiation. Mutation and/or translocation of FGFRs lead to aberrant signaling that often results in… (more)

▼ Fibroblast Growth Factor Receptors (FGFRs) are critical for cell proliferation and differentiation. Mutation and/or translocation of FGFRs lead to aberrant signaling that often results in developmental syndromes or cancer growth. As sequencing of human tumors becomes more frequent, so does the emergence of FGFR translocations and fusion proteins. The research conducted in this work will focus on a frequently identified fusion protein between FGFR3 and transforming acidic coiled-coil containing protein 3 (TACC3). As detailed in this dissertation, it is apparent that the fused coiled-coil TACC3 domain results in constitutive phosphorylation of key activating FGFR3 tyrosine residues. The presence of the TACC coiled-coil domain leads to increased and altered levels of FGFR3 activation, fusion protein phosphorylation, MAPK pathway activation, nuclear localization, cellular transformation, and IL3-independent proliferation. Introduction of K508R FGFR3 kinase dead mutation abrogates these effects, except for nuclear localization which is due solely to the TACC3 domain. We further demonstrate that the oncogenic effects initiated by FGFR3-TACC3 are dependent on the overactivation of the MAPK pathway and localization of FGFR3-TACC3 to the secretory pathway or the plasma membrane. The activation of the MAPK pathway is essential for cell transformation but involvement in the cell cycle via the canonical TACC3 pathways is not. Additionally, we have shown that kinase inhibitors for MEK (Trametinib) and FGFR (BGJ398) are effective in blocking cell transformation and MAPK pathway upregulation. The need for precision medicine is evidenced by the different effects these inhibitors have against various FGFR3-TACC3 breakpoints. The existence of FGFR3-TACC3 fusions in human cancers creates additional challenges and opportunities for identifying effective treatment strategies. The development of such personalized medicines will be essential in treating patients who harbor oncogenic drivers such as FGFR3-TACC3.

Subjects/Keywords: Biochemistry; Cellular biology; cancer; FGFR; fusion protein; TACC; translocation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nelson, K. (2018). Functional Characterization of Oncogenic Driver FGFR3-TACC3. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/9tj8f2k3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nelson, Katelyn. “Functional Characterization of Oncogenic Driver FGFR3-TACC3.” 2018. Thesis, University of California – San Diego. Accessed April 18, 2021. http://www.escholarship.org/uc/item/9tj8f2k3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nelson, Katelyn. “Functional Characterization of Oncogenic Driver FGFR3-TACC3.” 2018. Web. 18 Apr 2021.

Vancouver:

Nelson K. Functional Characterization of Oncogenic Driver FGFR3-TACC3. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Apr 18]. Available from: http://www.escholarship.org/uc/item/9tj8f2k3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nelson K. Functional Characterization of Oncogenic Driver FGFR3-TACC3. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/9tj8f2k3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

California State University – Northridge

19. Maly, Jan. Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies.

Degree: MS, Chemistry and Biochemistry, 2013, California State University – Northridge

URL: http://hdl.handle.net/10211.2/3679

► Cellular signaling is one the hallmarks of multicellular organisms. The transmission of information between cells enables organisms to not only respond to their surrounding environment,… (more)

▼ Cellular signaling is one the hallmarks of multicellular organisms. The transmission of information between cells enables organisms to not only respond to their surrounding environment, but also to learn from it. The activation of the majority of these pathways involves a relay of information from external stimuli to the cell surface, and the transmission of that information from the cell surface to internal effector systems. In addition to small molecule messengers, protein-protein interactions comprise a vast number of the signaling interactions that take place to convey information along these pathways. Unlike their small molecule counterparts, however, proteins can act as both messengers and receptors, and utilize internal motions and structural changes to carry out these activities. In our laboratory, we use nuclear magnetic resonance (NMR) to study how these inter- and intramolecular protein motions affect the specificity in protein-protein interactions, and their role in protein signaling. This thesis focuses on two types of signaling proteins: the human G-protein inhibitory subunit alpha i 1 (hG??i1), and human neurotrophin-4 (hNT4). The heterotrimeric G protein complex (composed of ??, ??, and ?? subunits) is involved in the activation and inactivation of many critical signal transmission cascades; malfunctions of these proteins have been implicated in numerous diseases, including depression, cancer and cardiovascular disease. As part of a longer-term investigation of intramolecular protein motions in regulators of G-protein signaling (RGS) and G?? proteins in their apo and complexed forms, we have successfully developed a protocol for preparing milligram quantities of highly purified, isotopically labeled wild-type human G??i1 (hG??i1) subunit for NMR studies. High levels of protein expression in E. coli can be attributed to the use of the SUMO fusion protein system, a bacterial strain that produces rare codons, supplementation of minimal medium with small quantities of isotopically labeled rich medium and a lowered temperature during induction. Purification of hG??i1 utilized both affinity and size exclusion chromatography, and protein activity was confirmed using fluorescence-based GTP-binding studies. Preliminary NMR analysis of 15N-labeled hG??i1 has shown that high-quality spectra can be obtained at near-physiological temperatures, whereas lower temperature spectra are characterized by numerous weak and broadened peaks, providing preliminary evidence for extensive ??s-ms timescale exchange throughout the subunit. In an effort to further optimize the NMR spectra we prepared a truncated form of hG??i1 (hG??i1-??31) in which the 31-residue unstructured N-terminus was removed. This resulted in further improvements in spectral quality by eliminating high-intensity peaks at the center of the spectrum that were obscuring resonances from structured segments of the protein. We plan to use this N-terminally truncated form of wild-type human G??i1 in future investigations of protein dynamics by NMR… Advisors/Committee Members: Crowhurst, Karin A. (advisor), Fischhaber, Paula L. (committee member).

Subjects/Keywords: fusion protein; Dissertations, Academic – CSUN – Chemistry and Biochemistry – Biochemistry.

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APA (6^th Edition):

Maly, J. (2013). Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies. (Masters Thesis). California State University – Northridge. Retrieved from http://hdl.handle.net/10211.2/3679

Chicago Manual of Style (16^th Edition):

Maly, Jan. “Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies.” 2013. Masters Thesis, California State University – Northridge. Accessed April 18, 2021. http://hdl.handle.net/10211.2/3679.

MLA Handbook (7^th Edition):

Maly, Jan. “Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies.” 2013. Web. 18 Apr 2021.

Vancouver:

Maly J. Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies. [Internet] [Masters thesis]. California State University – Northridge; 2013. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10211.2/3679.

Council of Science Editors:

Maly J. Integral role of the SUMO fusion protein system in successful expression and purification of two difficult proteins for NMR studies. [Masters Thesis]. California State University – Northridge; 2013. Available from: http://hdl.handle.net/10211.2/3679

Northeastern University

20. Kates, Sydney. Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis.

Degree: MS, Department of Bioengineering, 2019, Northeastern University

URL: http://hdl.handle.net/2047/D20317934

► Osteoarthritis (OA) is a degenerative disease of the whole joint, affecting millions worldwide. Over time, the cartilage, synovium, and bone can degrade, leading to severe… (more)

▼ Osteoarthritis (OA) is a degenerative disease of the whole joint, affecting millions worldwide. Over time, the cartilage, synovium, and bone can degrade, leading to severe inflammation and pain. Treatments for OA are for remediating pain and are not effective at a long-term scale. To create a more effective treatment, it is necessary to improve drug delivery. The way we hope to do this is by using charge interactions with cartilage and a drug carrier to create a long lasting, drug depot within the highly negatively charged cartilage. For the drug carrier, we use Avidin (net charge +20), and for therapeutics, we use Interleuin-1 Receptor Antagonist (IL-1RA) or Insulin-like Growth Factor 1 (IGF-1). Avidin has been proven as a good carrier for OA drugs as there is a high retention time in cartilage. Both IL-1RA and IGF-1 have been proven to reduce or halt the progression of OA. To create a therapeutic that is bound to a drug carrier, we used well-established bacterial expression systems. We designed an insert to produce our genes of interest with the incorporation of additional elements to allow for efficient use of the bacterial vector. We incorporated restriction enzyme sites that allow for replacement of the therapeutic gene of interest to allow for production of another desired compound. Restriction sites are also present to allow for creation of controls for these therapeutics where they are not bound to the Avidin carrier. A His tag was incorporated for purification steps, and a flexible linker was chosen to allow for activity at the binding sites of protein. We incorporated the DNA of interest into different bacterial vectors and E.coli cell lines to create our protein. For cloning, we used the pUC57-Kan vector and DH5α cells. For protein production, we used the pET11 vector and BL21 cells. We were able to produce what we believe to be a monomeric Avidin bound to IL-1RA, and the IL-1RA control. Due to difficulty of assaying the protein, we were not able to confirm that Avidin had formed its native tetrameric structure. Further assaying and in vitro testing will assess the protein efficacy.

Subjects/Keywords: Drug Delivery; Fusion Protein; Molecular Cloning; Osteoarthritis; Biomedical engineering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kates, S. (2019). Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20317934

Chicago Manual of Style (16^th Edition):

Kates, Sydney. “Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis.” 2019. Masters Thesis, Northeastern University. Accessed April 18, 2021. http://hdl.handle.net/2047/D20317934.

MLA Handbook (7^th Edition):

Kates, Sydney. “Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis.” 2019. Web. 18 Apr 2021.

Vancouver:

Kates S. Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis. [Internet] [Masters thesis]. Northeastern University; 2019. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2047/D20317934.

Council of Science Editors:

Kates S. Design And Production Of A Fusion Protein For The Treatment Of Osteoarthritis. [Masters Thesis]. Northeastern University; 2019. Available from: http://hdl.handle.net/2047/D20317934

Colorado State University

21. Walker, Susanne N. Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1.

Degree: PhD, Chemistry, 2019, Colorado State University

URL: http://hdl.handle.net/10217/195296

► Methods for the stabilization of well-defined helical peptide drugs and basic research tools have received considerable attention in the last decade. Enfuvirtide is a 36-residue… (more)

Subjects/Keywords: HIV-1; protein engineering; membrane fusion; helix-grafting

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Walker, S. N. (2019). Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/195296

Chicago Manual of Style (16^th Edition):

Walker, Susanne N. “Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1.” 2019. Doctoral Dissertation, Colorado State University. Accessed April 18, 2021. http://hdl.handle.net/10217/195296.

MLA Handbook (7^th Edition):

Walker, Susanne N. “Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1.” 2019. Web. 18 Apr 2021.

Vancouver:

Walker SN. Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1. [Internet] [Doctoral dissertation]. Colorado State University; 2019. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10217/195296.

Council of Science Editors:

Walker SN. Engineering and evolving helical proteins that improve in vivo stability and inhibit entry of Enfuvirtide-resistant HIV-1. [Doctoral Dissertation]. Colorado State University; 2019. Available from: http://hdl.handle.net/10217/195296

University of Adelaide

22. Charlton, Adam. Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli.

Degree: 2008, University of Adelaide

URL: http://hdl.handle.net/2440/59637

► The recombinant expression of heterologous proteins in microorganisms, such Escherichia coli, is often improved by producing the protein of interest translationally linked to another, often… (more)

▼ The recombinant expression of heterologous proteins in microorganisms, such Escherichia coli, is often improved by producing the protein of interest translationally linked to another, often unrelated, protein giving rise to a "fusion protein" construct. For many applications it is desirable or imperative to separate the extraneous material from the protein of interest. An increasingly popular approach to this task is the use of site-specific endoproteases 10 excise the protein product. A number of commercially available site-specific proteases exist, but many are not capable of generating an authentic N-terminus for the product, display unsatisfactory specificity leading to adventitious cleavage of the product, or they are unsuitable for an industrial process. Mutants of the serine protease a-Lytic protease have been shown to satisfy many of the criteria for an industrially suitable protease and have been applied to the cleavage of some important fusion proteins used in the production of members of the Insulin-like Growth Factor (IGF) family Lacking from these examples, however, is any viable proteolytic solution for the liberation of human IGF-I from fusion proteins. This has been primarily attributed to the Proline bearing N-terminal tripeptide sequence of this protein, which is known to be refractory to the activity of many site-specific proteases. It has been suggested that in the generation of two combinatorial mutant libraries of a-Lytic protease, the preference for amino acids C-terminal to the cleavage site may have been altered. It is the purpose of this work to first determine if such an alteration has been made in any of the mutants so as to allow cleavage immediately before the N-terminus of human IGF-1, and then to task the lead mutant(s) to the cleavage of the full-length fusion protein. All members of the two mutant libraries were cultured and their activity confirmed and quantified against a generic B-casein substrate in a high-throughput assay. A second high-throughput technique was then employed to query the mutant proteases for their ability to catalyse proteolysis at the required sequence in a peptide model. Finding that many mutants appeared successful at this task, the findings were verified on a longer peptide model of the cleavage site. Initially the yields achieved by cleavage of the full-length IGF-1 fusion protein by a lead candidate mutant a-Lytic protease were not sufficient to satisfy the requirements of an industrial process, despite alteration of the reaction conditions. However, the insight gained from these reactions could be applied to the redesign of the protein structure around the intended site of cleavage, significantly improving site-specific proteolysis. The IGF-1 generated by this cleavage has been shown to be bioequivalent to commercial reference standard to cultured mammalian cells and the yield of this process is approximately 5-fold improved over the existing cleavage system. Advisors/Committee Members: School of Molecular and Biomedical Science : Biochemistry (school).

Subjects/Keywords: biopharmaceuticals; fusion; protein; escherichia coli

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APA (6^th Edition):

Charlton, A. (2008). Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/59637

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Charlton, Adam. “Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli.” 2008. Thesis, University of Adelaide. Accessed April 18, 2021. http://hdl.handle.net/2440/59637.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Charlton, Adam. “Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli.” 2008. Web. 18 Apr 2021.

Vancouver:

Charlton A. Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli. [Internet] [Thesis]. University of Adelaide; 2008. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2440/59637.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Charlton A. Improved production of biopharmaceuticals by site-specific cleavage of fusion proteins expressed in Escherichia coli. [Thesis]. University of Adelaide; 2008. Available from: http://hdl.handle.net/2440/59637

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Australian National University

23. Porter, Joanne Loren. Improving enzyme properties through directed evolution .

Degree: 2015, Australian National University

URL: http://hdl.handle.net/1885/110866

► Enzymes are specific and economical biocatalysts and as such are highly desirable synthetic tools. While there are many examples of successful applications of enzymes into… (more)

▼ Enzymes are specific and economical biocatalysts and as such are highly desirable synthetic tools. While there are many examples of successful applications of enzymes into industrial or commercial processes, the vast potential of enzyme technology is yet to be reached. Protein engineering, specifically directed evolution, is a powerful tool for generating enzymes tailored for specific industrial tasks. The ease of this process dictates the availability of enzymes and their diversity as biocatalysts. This thesis is concerned with the use of directed evolution to enhance the physical and catalytic properties of dienelactone hydrolase, a small monomeric alpha/beta hydrolase fold enzyme. Solubility is an important property dictating the suitability of enzymes as industrial biocatalysts in terms of both cost and functional utility. This thesis describes the adaption and use of the dihydrofolate reductase fusion reporter system to select for more soluble dienelactone hydrolase variants. The selection system was modified to incorporate a pre-culturing period, then used to identify mutations in solvent accessible locations that appeared to offer increased expression and solubility. While the original system was capable of selecting for improvements to the solubility of inherently insoluble proteins, the modified system can be used to select for further improvements. This work provides a solid foundation for the continued use of the modified dihydrofolate reductase fusion reporter system. This thesis also discusses linked directed evolution experiments that were designed to rapidly alter and enhance the substrate specificity of dienelactone hydrolase in favour of non-physiological p-nitrophenyl ester substrates. The best variants possessed in excess of 2000-fold improvements in kcat/Km compared to the native enzyme. Active site mutations were able to accumulate rapidly, despite most being detrimental to the overall stability of the enzyme, due to constant monitoring and maintenance of enzyme stability. The roles of six of the seven active site mutations were elucidated with the use of substrate docking and structural analysis. Additional work focused on the surface mutations that seemed to provide compensatory stabilising effects. These mutations were made individually and in combination to determine the roles they served in the evolution process. Two of the three mutations were shown to be thermally and chemically stabilising while the third was destabilising individually but exhibited epistasis in combination with the former mutations. This work is of interest due to the evolutionary strategy but more importantly for the insight into the evolution and relationship between sequence, structure and function of alpha/beta hydrolase fold enzymes. Work investigating and improving the stability of dienelactone hydrolase in the presence of organic co-solvents is also discussed. Eight rounds of directed evolution yielded a variant with 7 surface mutations that provided increased thermal and chemical stability. These experimental…

Subjects/Keywords: enzyme; biocatalyst; synthetic; dienelactone hydrolase; engineering; protein; dihydrofolate; reductase; fusion

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Porter, J. L. (2015). Improving enzyme properties through directed evolution . (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/110866

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Porter, Joanne Loren. “Improving enzyme properties through directed evolution .” 2015. Thesis, Australian National University. Accessed April 18, 2021. http://hdl.handle.net/1885/110866.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Porter, Joanne Loren. “Improving enzyme properties through directed evolution .” 2015. Web. 18 Apr 2021.

Vancouver:

Porter JL. Improving enzyme properties through directed evolution . [Internet] [Thesis]. Australian National University; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1885/110866.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Porter JL. Improving enzyme properties through directed evolution . [Thesis]. Australian National University; 2015. Available from: http://hdl.handle.net/1885/110866

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Princeton University

24. Baker, Richard Wayne. A direct for for SM proteins as templates for SNARE assembly .

Degree: PhD, 2015, Princeton University

URL: http://arks.princeton.edu/ark:/88435/dsp011v53k0363

► Intracellular membrane trafficking depends on the concerted action of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) that physically drive membrane fusion. Membrane-bridging SNARE complexes are… (more)

Subjects/Keywords: HOPS complex; Membrane Fusion; Membrane Trafficking; SM protein; SNARE

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APA (6^th Edition):

Baker, R. W. (2015). A direct for for SM proteins as templates for SNARE assembly . (Doctoral Dissertation). Princeton University. Retrieved from http://arks.princeton.edu/ark:/88435/dsp011v53k0363

Chicago Manual of Style (16^th Edition):

Baker, Richard Wayne. “A direct for for SM proteins as templates for SNARE assembly .” 2015. Doctoral Dissertation, Princeton University. Accessed April 18, 2021. http://arks.princeton.edu/ark:/88435/dsp011v53k0363.

MLA Handbook (7^th Edition):

Baker, Richard Wayne. “A direct for for SM proteins as templates for SNARE assembly .” 2015. Web. 18 Apr 2021.

Vancouver:

Baker RW. A direct for for SM proteins as templates for SNARE assembly . [Internet] [Doctoral dissertation]. Princeton University; 2015. [cited 2021 Apr 18]. Available from: http://arks.princeton.edu/ark:/88435/dsp011v53k0363.

Council of Science Editors:

Baker RW. A direct for for SM proteins as templates for SNARE assembly . [Doctoral Dissertation]. Princeton University; 2015. Available from: http://arks.princeton.edu/ark:/88435/dsp011v53k0363

University of Southern California

25. Wang, Yan. Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment.

Degree: PhD, Molecular Pharmacology and Toxicology, 2013, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/91307/rec/5274

► Long-acting insulin (INS) analogues that exhibit prolonged time-action profiles and liver-specificity are currently in great demand for diabetes treatment. Native INS and its protein precursor… (more)

▼ Long-acting insulin (INS) analogues that exhibit prolonged time-action profiles and liver-specificity are currently in great demand for diabetes treatment. Native INS and its protein precursor proinsulin (ProINS) are both small peptides with short in vivo half-life and efficacy. Human transferrin (Tf) is a stable and large-sized plasma protein, and it has been demonstrated to prolong the half-life of small proteins. With the purposes of improving the therapeutic application of INS, a proinsulin-transferrin (ProINS-Tf) recombinant fusion protein has been designed and developed. This fusion protein is produced using recombinant fusion technology combined with his-tag purification method. ProINS-Tf exhibits a low activity in the 30 min promotion of glucose uptake in adipocytes, which corresponds with a low binding affinity to insulin receptor. Additionally, ProINS-Tf can be activated by ex vivo trypsin digestion. These results suggest that, similar to ProINS, the intrinsic potency of ProINS-Tf is low and an activation is required to achieve biological activity. On the other hand, ProINS-Tf elicits a Tf receptor (TfR) dependent enhanced activity in the 24 h inhibition of glucose production in hepatoma cells. Radioimmunoassays clearly demonstrate a TfR-mediated conversion and activation of ProINS-Tf to an immunoreactive insulin-transferrin fusion protein during the 24 h incubation with hepatoma cells. Therefore, we have proposed an intracellular ProINS-Tf activation mechanism that is mediated through TfR-mediated endocytosis and recycling pathway. Furthermore, compared to ProINS and INS, subcutaneously injected ProINS-Tf exerts an extended hypoglycemic efficacy with a prolonged half-life in fasted diabetic mice. The correlation of hypoglycemic efficacy with the suppression of liver-associated enzyme expression suggests a liver-preferential effect by ProINS-Tf. In addition, intravenously injection of trypsin-digested ProINS-Tf shows an immediate hypoglycemic response, whereas ProINS-Tf exhibits a delayed and long-lasting hypoglycemic efficacy. These observations imply that an activation of ProINS-Tf may also occur in vivo. Taken together, results from this dissertation have presented three contributions to the therapeutic protein discovery and development of. First, ProINS-Tf is a novel fusion protein and the first proprotein within the Tf-based fusion protein family. Second, a novel receptor-mediated intracellular proprotein activation mechanism is discovered. Lastly, ProINS-Tf shows great promise as a potential long-acting INS analogue for diabetes treatment. Advisors/Committee Members: Shen, Wei-Chiang (Committee Chair), Stiles, Bangyan L. (Committee Member), Wang, Clay C.C. (Committee Member), Haworth, Ian S. (Committee Member).

Subjects/Keywords: transferrin; insulin; proinsulin; prodrug activation; recombinant fusion protein; diabetes

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APA (6^th Edition):

Wang, Y. (2013). Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/91307/rec/5274

Chicago Manual of Style (16^th Edition):

Wang, Yan. “Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment.” 2013. Doctoral Dissertation, University of Southern California. Accessed April 18, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/91307/rec/5274.

MLA Handbook (7^th Edition):

Wang, Yan. “Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment.” 2013. Web. 18 Apr 2021.

Vancouver:

Wang Y. Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment. [Internet] [Doctoral dissertation]. University of Southern California; 2013. [cited 2021 Apr 18]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/91307/rec/5274.

Council of Science Editors:

Wang Y. Proinsulin-transferrin recombinant fusion protein: mechanism of activation and potential application in diabetes treatment. [Doctoral Dissertation]. University of Southern California; 2013. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/91307/rec/5274

University of Southern California

26. Lee, Hsin-Fang. Preparation and characterization of Tf-G-CSF fusion protein.

Degree: MS, Molecular Pharmacology & Toxicology, 2008, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/89126/rec/5189

► In this study, Tf-H42-G-CSF fusion protein was engineered for investigating the effects of H4 helical linker 's on the fusion protein expression and the sequence… (more)

Subjects/Keywords: transferrin; G-CSF; fusion protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, H. (2008). Preparation and characterization of Tf-G-CSF fusion protein. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/89126/rec/5189

Chicago Manual of Style (16^th Edition):

Lee, Hsin-Fang. “Preparation and characterization of Tf-G-CSF fusion protein.” 2008. Masters Thesis, University of Southern California. Accessed April 18, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/89126/rec/5189.

MLA Handbook (7^th Edition):

Lee, Hsin-Fang. “Preparation and characterization of Tf-G-CSF fusion protein.” 2008. Web. 18 Apr 2021.

Vancouver:

Lee H. Preparation and characterization of Tf-G-CSF fusion protein. [Internet] [Masters thesis]. University of Southern California; 2008. [cited 2021 Apr 18]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/89126/rec/5189.

Council of Science Editors:

Lee H. Preparation and characterization of Tf-G-CSF fusion protein. [Masters Thesis]. University of Southern California; 2008. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/89126/rec/5189

27. Schoeben, Melissa A. Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans.

Degree: MSin Biology, Biology, 2017, Missouri State University

URL: https://bearworks.missouristate.edu/theses/3117

► The acetic acid bacterium Gluconobacter oxydans is an industrially valuable microorganism, particularly in the production of acetic acid, D-gluconic acid, ketogluconic acids, dihydroxyacetone, and… (more)

Subjects/Keywords: Gluconobacter oxydans; fluorescent reporter; inducible promoter; fusion protein; genetic tools; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schoeben, M. A. (2017). Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans. (Masters Thesis). Missouri State University. Retrieved from https://bearworks.missouristate.edu/theses/3117

Chicago Manual of Style (16^th Edition):

Schoeben, Melissa A. “Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans.” 2017. Masters Thesis, Missouri State University. Accessed April 18, 2021. https://bearworks.missouristate.edu/theses/3117.

MLA Handbook (7^th Edition):

Schoeben, Melissa A. “Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans.” 2017. Web. 18 Apr 2021.

Vancouver:

Schoeben MA. Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans. [Internet] [Masters thesis]. Missouri State University; 2017. [cited 2021 Apr 18]. Available from: https://bearworks.missouristate.edu/theses/3117.

Council of Science Editors:

Schoeben MA. Genetic Tools to Allow Efficient Gene and Protein Characterization of the Industrially Important Bacterium Gluconobacter Oxydans. [Masters Thesis]. Missouri State University; 2017. Available from: https://bearworks.missouristate.edu/theses/3117

The Ohio State University

28. Chaiwatpongsakorn, Supranee. Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering.

Degree: PhD, Comparative and Veterinary Medicine, 2011, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1307730650

► Respiratory syncytial virus (RSV), a member of the Paramyxoviridae family, Pneumovirinae subfamily is the most significant respiratory pathogen in infants and second only to influenza… (more)

Subjects/Keywords: Virology; RSV; fusion protein; triggering

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chaiwatpongsakorn, S. (2011). Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1307730650

Chicago Manual of Style (16^th Edition):

Chaiwatpongsakorn, Supranee. “Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering.” 2011. Doctoral Dissertation, The Ohio State University. Accessed April 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1307730650.

MLA Handbook (7^th Edition):

Chaiwatpongsakorn, Supranee. “Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering.” 2011. Web. 18 Apr 2021.

Vancouver:

Chaiwatpongsakorn S. Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering. [Internet] [Doctoral dissertation]. The Ohio State University; 2011. [cited 2021 Apr 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1307730650.

Council of Science Editors:

Chaiwatpongsakorn S. Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State, a Tool to Study Protein Triggering. [Doctoral Dissertation]. The Ohio State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1307730650

University of Kentucky

29. Klimyte, Edita M. ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS.

Degree: 2016, University of Kentucky

URL: https://uknowledge.uky.edu/biochem_etds/28

► Human metapneumovirus (HMPV) is a respiratory pathogen in the Paramyxoviridae family that infects nearly 100% of the world population. This enveloped RNA virus causes severe… (more)

▼ Human metapneumovirus (HMPV) is a respiratory pathogen in the Paramyxoviridae family that infects nearly 100% of the world population. This enveloped RNA virus causes severe viral respiratory disease in infants, the elderly, and immunocompromised patients worldwide. Despite its prevalence and importance to human health, no therapies are available against this pathogen. Entry of paramyxoviruses into host cells generally requires the coordinated activity of the attachment glycoprotein, G, which interacts with a cell receptor, and the fusion glycoprotein, F, which promotes subsequent fusion of viral and cellular membranes. However, HMPV F is the primary viral protein mediating both binding and fusion for HMPV. Previous work that showed HMPV F mediates attachment to heparan sulfate proteoglycans (HSPGs), and some HMPV F fusion activity can be promoted by acidic pH. The work presented here provides significant advances in our understanding of the fusion and binding events during HMPV infection. We demonstrated that low pH promotes fusion in HMPV F proteins from diverse clades, challenging previously reported requirements and identifying a critical residue that enhances low pH promoted fusion. These results support our hypothesis that electrostatic interactions play a key role in HMPV F triggering and further elucidate the complexity of viral fusion proteins. Additionally, we characterized the key features of the binding interaction between HMPV and HSPGs using heparan sulfate mimetics, identifying an important sulfate modification, and demonstrated that these interactions occur at the apical surface of polarized airways tissues. We identified differences in particle binding related to the presence or absence of the HMPV G and SH glycoproteins. Lastly, we characterized paramyxovirus infection in cystic fibrosis bronchial epithelial cells, identifying a potential specific susceptibility to HMPV infection in these individuals. The work presented here contributes to our understanding of HMPV infection, from mechanisms of early events of entry to clinical scenarios.

Subjects/Keywords: Paramyxovirus; Human Metapneumovirus; Fusion Protein Membrane Fusion; Binding; Heparan Sulfate; Cystic Fibrosis; Other Immunology and Infectious Disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klimyte, E. M. (2016). ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/biochem_etds/28

Chicago Manual of Style (16^th Edition):

Klimyte, Edita M. “ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS.” 2016. Doctoral Dissertation, University of Kentucky. Accessed April 18, 2021. https://uknowledge.uky.edu/biochem_etds/28.

MLA Handbook (7^th Edition):

Klimyte, Edita M. “ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS.” 2016. Web. 18 Apr 2021.

Vancouver:

Klimyte EM. ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS. [Internet] [Doctoral dissertation]. University of Kentucky; 2016. [cited 2021 Apr 18]. Available from: https://uknowledge.uky.edu/biochem_etds/28.

Council of Science Editors:

Klimyte EM. ELUCIDATING BINDING, FUSION AND ENTRY OF HUMAN METAPNEUMOVIRUS. [Doctoral Dissertation]. University of Kentucky; 2016. Available from: https://uknowledge.uky.edu/biochem_etds/28

30. 佐藤, 友人. 麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus.

Degree: 博士（バイオサイエンス）, 2015, Nagahama Institute of Bio-Science and Technology / 長浜バイオ大学

URL: http://id.nii.ac.jp/1211/00000017/

2014

Subjects/Keywords: Measles virus; Fusion protein; Membrane fusion; Refolding; Thermodynamic stability; Measles virus; Fusion protein; Membrane fusion; Refolding; Thermodynamic stability

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

佐藤, �. (2015). 麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus. (Thesis). Nagahama Institute of Bio-Science and Technology / 長浜バイオ大学. Retrieved from http://id.nii.ac.jp/1211/00000017/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

佐藤, 友人. “麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus.” 2015. Thesis, Nagahama Institute of Bio-Science and Technology / 長浜バイオ大学. Accessed April 18, 2021. http://id.nii.ac.jp/1211/00000017/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

佐藤, 友人. “麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus.” 2015. Web. 18 Apr 2021.

Vancouver:

佐藤 �. 麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus. [Internet] [Thesis]. Nagahama Institute of Bio-Science and Technology / 長浜バイオ大学; 2015. [cited 2021 Apr 18]. Available from: http://id.nii.ac.jp/1211/00000017/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

佐藤 �. 麻疹ウイルスF蛋白の膜融合活性発現の分子機構 : Molecular mechanism of expression of membranefusion activity by fusion protein of measles virus. [Thesis]. Nagahama Institute of Bio-Science and Technology / 長浜バイオ大学; 2015. Available from: http://id.nii.ac.jp/1211/00000017/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations