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You searched for subject:(fura 2 AM). Showing records 1 – 3 of 3 total matches.

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Universiteit Utrecht

1. Daniël, J.W. Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.

Degree: 2014, Universiteit Utrecht

Kisspeptin is an important regulator of the hypothalamic-pituitary-gonadal axis in mammals. It acts on the GnRH neurons trough the GPR54 receptor initiating GnRH release. The aim of this study is to determine the optimal concentration of kisspeptin-10 to activate the GPR54 receptor in vitro. This data is then used for in vitro testing of the kisspeptin antagonist P271. The GPR54 receptor is a G-protein coupled receptor, which when activated raises the intracellular calcium level. We used a single-cell fluorescence microscopy on Chem-1 cells (expressing the human GPR54 receptor) loaded with the calcium-sensitive fluorescent dye Fura-2. We were unable to create a reproducible concentration-response study, however we found two concentrations of kisspeptin-10 that gave a reproducible peak (10-6M and 10-7M). These concentrations were used to test the antagonist P271. In this study peptide P271 showed agonistic properties and no antagonistic properties. This could be because of the method used in these experiments. Advisors/Committee Members: Albers-Wolthers, C.H.J..

Subjects/Keywords: Kisspeptin; GPR54; Antagonist; P271; in vitro; single-cell fluorescence microscopy; fura-2-AM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Daniël, J. W. (2014). Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289058

Chicago Manual of Style (16th Edition):

Daniël, J W. “Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.” 2014. Masters Thesis, Universiteit Utrecht. Accessed March 06, 2021. http://dspace.library.uu.nl:8080/handle/1874/289058.

MLA Handbook (7th Edition):

Daniël, J W. “Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271.” 2014. Web. 06 Mar 2021.

Vancouver:

Daniël JW. Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Mar 06]. Available from: http://dspace.library.uu.nl:8080/handle/1874/289058.

Council of Science Editors:

Daniël JW. Kisspeptin in vitro: a concentration response study and testing of kisspeptin antagonist P271. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/289058


University of the Western Cape

2. Egunlusi, Ayodeji Olatunde. Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection .

Degree: 2014, University of the Western Cape

This study focused on the synthesis of a series of novel tricycloundecane derivatives and evaluation of these compounds for neuroprotection using the fluorescent ratiometric calcium assay that indicates the ability of the test compounds to inhibit NMDA receptors and VGCC. The cycloaddition reaction between p-benzoquinone and monomerised dicyclopentadiene yielded tricycloundeca- 4,9-diene-3,6-dione which was used as the base structure and further derivatised. These derivatives were conjugated with benzylamine to form a series of imines and amines. A total of 10 compounds were synthesised for evaluation of inhibition of calcium influx through NMDA receptor channels and voltage-gated calcium channels. The structures were confirmed using NMR, IR and MS. On the proton NMR, the characteristic AB-quartet system was observed in the region of 1-2 ppm for all the compounds and the aromatic moiety was observed between 6.5-7.5 ppm for the novel polycyclic amines. These, with other functional groups, were used to confirm the individual structures Advisors/Committee Members: Joubert, Jacques (advisor), Malan, Sarel (advisor).

Subjects/Keywords: Neurodegenerative disorders; Tricycloundecane; N-methyl-D-aspartate receptor; Voltage-gated calcium channel; Oxidative stress; Synaptoneurosomes; Excitotoxicity; Apoptosis; Necrosis; Polycyclic cage; Neuroprotective agents; Fura-2 AM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Egunlusi, A. O. (2014). Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/3904

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Egunlusi, Ayodeji Olatunde. “Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection .” 2014. Thesis, University of the Western Cape. Accessed March 06, 2021. http://hdl.handle.net/11394/3904.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Egunlusi, Ayodeji Olatunde. “Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection .” 2014. Web. 06 Mar 2021.

Vancouver:

Egunlusi AO. Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection . [Internet] [Thesis]. University of the Western Cape; 2014. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/11394/3904.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Egunlusi AO. Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection . [Thesis]. University of the Western Cape; 2014. Available from: http://hdl.handle.net/11394/3904

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Université de Montréal

3. Genty, Hélène. Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries.

Degree: 2003, Université de Montréal

Subjects/Keywords: Récepteur du facteur autocrine de motilité; Réticulum endoplasmique lisse; Mitochondries; Sous-domaine; Calcium; Réserves calciques; Ionomycine; Thapsigargine; Fura-2-AM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Genty, H. (2003). Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries. (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/14627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Genty, Hélène. “Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries.” 2003. Thesis, Université de Montréal. Accessed March 06, 2021. http://hdl.handle.net/1866/14627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Genty, Hélène. “Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries.” 2003. Web. 06 Mar 2021.

Vancouver:

Genty H. Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries. [Internet] [Thesis]. Université de Montréal; 2003. [cited 2021 Mar 06]. Available from: http://hdl.handle.net/1866/14627.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Genty H. Étude de l'association du réticulum endoplasmique lisse marqué par le récepteur du facteur autocrine de motilité avec les mitochondries. [Thesis]. Université de Montréal; 2003. Available from: http://hdl.handle.net/1866/14627

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.