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You searched for subject:(fructan prebiotics). Showing records 1 – 2 of 2 total matches.

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University of Adelaide

1. Huynh, Bao Lam. Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.).

Degree: 2009, University of Adelaide

Fructans are polysaccharides that are made up mainly of fructose. They are non-digestible carbohydrates and act as prebiotics to selectively promote the growth of colonic bifidobacteria, thereby improving human gut health. Fructans are present in the grain of wheat (Triticum aestivum L.), a staple food crop. Until now, there has been no research on genetic improvement of the concentration of fructans in wheat grain, partly because it has been difficult to accurately measure. One aim of this research project was to develop a simple and effective method to measure the fructan concentration in wheat grain. This was achieved by modifying a method that involves extraction of fructans from wheat grain followed by enzymatic hydrolysis to break down fructans into monosaccharides and quantification by anion-exchange liquid chromatography coupled with pulsed amperometric detection. The modified procedure is reliable and allows the handling of large numbers of flour samples at a relatively low cost, and can therefore be useful for assessing large numbers of wheat breeding lines. Using this method, grain samples taken from a diverse set of 117 wheat cultivars and breeding lines, including parents of mapping populations, were analysed for grain fructan concentration. There was significant genotypic variation among these materials, with grain fructan concentration ranging from 0.3 to 2.3% of grain dry weight. There was no evidence of strong genotype-byenvironment interaction; the fructan concentrations of the same genotypes were positively correlated over different environments in Australia. Genetic mapping was carried out to detect and map loci affecting grain fructan concentration in wheat using a doubled haploid population derived from a cross between Berkut (high fructan) and Krichauff (low fructan). Grain samples were obtained from two field sites in South Australia and one in Kazakhstan. Fructan concentration varied widely within the population (0.6-2.6% of grain dry weight), with heritability estimated as h² = 0.71. A linkage map of 528 molecular markers covering 21 wheat chromosomes was used for locating quantitative trait loci (QTL). Genetic mapping identified two major QTLs on chromosomes 6D and 7A, with the (high fructan concentration) alleles contributed from Berkut, contributing to a 30-40% increase in wheat grain fructan compared to the Krichauff alleles. Effects of these chromosome regions were validated in additional environments and in another mapping population, Sokoll/Krichauff, with the favourable alleles contributed from Sokoll. The major QTL on chromosome 7A was in the same region with a reported fructosyltransferase orthologue (AB029888), while the major QTL on chromosome 6D seemed to be co-located with a reported gene encoding for a fructan-degrading enzyme 1-exohydrolase (1-FEHw2). It is concluded that grain fructan concentration of wheat can be improved by breeding and that molecular markers could be used to select effectively for favourable alleles in two regions of the wheat genome. Advisors/Committee Members: Wallwork, Hugh (advisor), Stangoulis, James Constantine Roy (advisor), Graham, Robin David (advisor), School of Agriculture, Food and Wine : Plant and Food Science (school).

Subjects/Keywords: fructan; biofortification; prebiotics; Quantitative Trait Loci; wheat; QTL; molecular cereal breeding; Wheat Genetics; Fructans

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Huynh, B. L. (2009). Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.). (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/52594

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Huynh, Bao Lam. “Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.).” 2009. Thesis, University of Adelaide. Accessed November 15, 2019. http://hdl.handle.net/2440/52594.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Huynh, Bao Lam. “Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.).” 2009. Web. 15 Nov 2019.

Vancouver:

Huynh BL. Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.). [Internet] [Thesis]. University of Adelaide; 2009. [cited 2019 Nov 15]. Available from: http://hdl.handle.net/2440/52594.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huynh BL. Genetic characterization and QTL mapping for grain fructan in wheat (Triticum aestivum L.). [Thesis]. University of Adelaide; 2009. Available from: http://hdl.handle.net/2440/52594

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Illinois – Urbana-Champaign

2. Cephas, Kimberly. Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice.

Degree: MS, 0191, 2012, University of Illinois – Urbana-Champaign

Obesity is linked to increased intestinal permeability that may contribute to low grade inflammation. Fructan prebiotics have been demonstrated to increase intestinal resistance and decrease systemic inflammation. The objective of this study was to test the effects of prebiotics on intestinal permeability, morphology, and gene expression in an obese mouse model. Obese, 18-wk old, C57BL/6 mice were randomized to high-fat (45% of kcal) diets containing 5% cellulose, 10% cellulose, 10% short-chain fructooligosaccharides (scFOS) or 10% inulin and fed for 28 d. Distal ileum, cecum, and colon samples were collected for Ussing chamber, histomorphology, and qRT-PCR analyses. The effects of treatment were tested using the Mixed Models procedure of SAS. Among treatments, mice fed scFOS and inulin had greater (p<0.05) intestinal transmural resistance compared to mice fed 5% cellulose. MCT-1 expression was greater (p<0.05) in the distal colon of mice fed 10% cellulose compared to 10% inulin. ZO-1 expression was lower (p<0.05) in mice fed inulin compared to other treatments. Occludin mRNA abundance was lowest (p<0.05) in fructan sources compared to cellulose. When comparing 5% vs. 10% cellulose treatments using contrasts, 10% cellulose led to greater (p<0.05) intestinal crypt depth in all intestinal regions, except the distal colon. Mice fed 5% vs. 10% cellulose, however, had greater (p<0.05) ileal villus height: crypt depth ratio, ileal MUC2 mRNA abundance, proximal colon AMPK mRNA abundance, and occludin mRNA abundance in the proximal and distal colon regions. When comparing fructan vs. cellulose treatments using contrasts, fructans resulted in a greater (p<0.05) transmural resistance and crypt depth when all intestinal regions were combined. In contrast, fructan-fed mice had lower (p<0.05) mRNA abundance of ZO-1 and occludin when all intestinal regions were combined (Table 3.5). Fructan consumption resulted in lower (p<0.05) ileal MUC2 and occludin mRNA abundance, cecal occludin mRNA abundance, proximal colon MCT-1, ZO-1, AMPK, and occludin mRNA abundance, and distal colon ZO-1, AMPK, and occludin mRNA abundance. Cecal AMPK mRNA abundance was greater (p<0.05) in fructan-fed compared to cellulose-fed mice. Pearson coefficient correlations indicated correlations between MCT-1 and distal colon ZO-1 (r = 0.77, p<0.05) and occludin mRNA abundance (r = 0.66, p<0.05). AMPK correlated with ZO-1 mRNA abundance (r = > 0.60, p<0.05) in all regions of the intestinal tissue of C57BL/6 mice. Lastly, ZO-1 mRNA abundance correlated with distal colon epithelial resistance (r = 0.51, p<0.05). Collectively, these data may suggest mechanisms by which equivalent quantities of fructan prebiotics and non-fermentable fibers affect intestinal barrier function. Advisors/Committee Members: Swanson, Kelly S. (advisor).

Subjects/Keywords: solute carrier family 5, member 8 (SLC5A8); monocarboxylic transporter-1 (MCT-1); monocarboxylic transporter-4 (MCT-4); solute carrier family 5, member 12 (SLC5A12); 5' AMP-activated protein kinase (AMPK); G-protein coupled receptor-43 (GPR43); mucin 2 (MUC2); zonula occluden-1 (ZO-1); taste receptor type 2-38 (T2R38); obesity and intestinal barrier function; obesity and gut permeability; obesity; obese rodent model; inflammation; fructan prebiotics; inulin; short-chain fructooligosaccharides (scFOS); crypt depth; villus height

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cephas, K. (2012). Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/32026

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Cephas, Kimberly. “Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice.” 2012. Thesis, University of Illinois – Urbana-Champaign. Accessed November 15, 2019. http://hdl.handle.net/2142/32026.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Cephas, Kimberly. “Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice.” 2012. Web. 15 Nov 2019.

Vancouver:

Cephas K. Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2012. [cited 2019 Nov 15]. Available from: http://hdl.handle.net/2142/32026.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cephas K. Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice. [Thesis]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/32026

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.