subject:(fluorescence imaging)

1. Valm, Alex Mihkel. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.

Degree: PhD, Pathobiology, 2012, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:297560/

► Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different… (more)

▼ Just as the phenotypically different cells that make up multicellular organisms are distributed in tissues with structures that embody specific functions, microbial cells with different metabolic functions form unique spatial structures and coordinate their activities as multicellular units, e.g. biofilms. Fundamentally, microbial communities differ from eukaryotic tissues because their cellular constituents may be genetically distinct; in fact, up to hundreds of different species may be present in a single biofilm. Any number of probes may be designed to identify the different species present in a community; however, the ability to unambiguously distinguish more than a few different labels in a single fluorescence image has been severely hampered by the excitation cross talk and signal bleed-through of fluorophores with highly overlapping emission spectra. Reported in this thesis is a fluorescence labeling, imaging, and analysis method to greatly expand the number of identifiable labels in a single image, which we call Combinatorial Labeling and Spectral Imaging (CLASI). Application of our CLASI technique to human dental plaque using fluorescence in situ hybridization (FISH) enabled the first quantitative analysis of the spatial distribution of 15 different taxa of microbes in a biofilm. Proximity analysis was used to determine the frequency of inter- and intrataxon cell-to-cell associations, which revealed statistically significant intertaxon pairings. Cells of the genera Prevotella and Actinomyces showed the most interspecies associations, suggesting a central role for these genera in establishing and maintaining biofilm complexity. In a proof-of-principle experiment, we further demonstrate that we can distinguish 120 differently labeled E. coli in a mixture labeled with binary combinations of 16 fluorophores using a novel linear unmixing algorithm constrained to identify specific combinations of fluorophores. Our results provide an initial systems-level structural analysis of biofilm organization and we further believe that the CLASI approach will be useful for the architectural analysis of many complex molecular structures within cells. Advisors/Committee Members: Oldenbourg, Rudolf (Director), Borisy, Gary (Director), Atwood, Walter (Reader), Reichner, Jonathan (Reader), Danuser, Gaudenz (Reader).

Subjects/Keywords: fluorescence imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Valm, A. M. (2012). Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. (Doctoral Dissertation). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:297560/

Chicago Manual of Style (16^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Doctoral Dissertation, Brown University. Accessed January 21, 2021. https://repository.library.brown.edu/studio/item/bdr:297560/.

MLA Handbook (7^th Edition):

Valm, Alex Mihkel. “Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization.” 2012. Web. 21 Jan 2021.

Vancouver:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Internet] [Doctoral dissertation]. Brown University; 2012. [cited 2021 Jan 21]. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/.

Council of Science Editors:

Valm AM. Combinatorial Labeling and Spectral Imaging (CLASI): A novel microscopy method for the systems-level analysis of biological structure and its application to the study of human oral microbial community organization. [Doctoral Dissertation]. Brown University; 2012. Available from: https://repository.library.brown.edu/studio/item/bdr:297560/

NSYSU

2. Chen, Yu-wei. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.

Degree: PhD, Chemistry, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

► To reduce the image blurring which originates from contributions of a cylindrical array of photolysis events in a photo-induced experiment, a variant of fluorescence imaging… (more)

Subjects/Keywords: Sliced fluorescence imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, Y. (2009). Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

Chicago Manual of Style (16^th Edition):

Chen, Yu-wei. “Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.” 2009. Doctoral Dissertation, NSYSU. Accessed January 21, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557.

MLA Handbook (7^th Edition):

Chen, Yu-wei. “Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes.” 2009. Web. 21 Jan 2021.

Vancouver:

Chen Y. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. [Internet] [Doctoral dissertation]. NSYSU; 2009. [cited 2021 Jan 21]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557.

Council of Science Editors:

Chen Y. Sliced fluorescence imaging: a versatile method to study photo-induced dynamic processes. [Doctoral Dissertation]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-155557

University of California – Berkeley

3. Srikun, Duangkhae. New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells.

Degree: Chemistry, 2011, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/4cj4q4xv

► As one of the toxic by-product of aerobic metabolism, hydrogen peroxide (H2O2), at uncontrolled levels and distributions, is a sign of oxidative stress, aging and… (more)

▼ As one of the toxic by-product of aerobic metabolism, hydrogen peroxide (H2O2), at uncontrolled levels and distributions, is a sign of oxidative stress, aging and disease. However, H2O2 also plays an essential part in normal physiological system. H2O2 levels are regulated by many enzymes and metabolites that generate or break-down H2O2. In macrophages, the presence of invading pathogens activates the production of microbicidal levels of H2O2 by NADPH oxidase (Nox). Isoforms of Nox are expressed in many non-phagocytic cells and tissues. Nox-generated H2O2 is a secondary messenger involved in signaling for growth, proliferation, differentiation and controlled cell death; these variations in downstream biological effects are regulated by both the spatial and temporal production of H2O2. Small molecule fluorescent probes bearing boronate ester moieties have been developed for chemoselective detection of H2O2 in both oxidative stress levels and cellular signaling events. This dissertation describes the design, synthesis, characterization and application of new boronate-based fluorescent probes with added functionality. Peroxy-Lucifer-1 (PL1) and Peroxy-Naphthalene-1 (PN1) are ratiometric fluorescent probes that can detect oxidative bursts in immune response events. Ratiometric probes allow simultaneous detection of two signals from the reacted and unreacted probes in the same sample, providing a built-in correction for variations such as uneven probe loading, sample environment and detection efficiency. PN1 also has a high two-photon cross section. The increased penetration depth of near-infrared excitation light allows the detection of H2O2 in tissue specimens with PN1. SNAP-Peroxy-Green-1 (SPG1) and SNAP-Peroxy-Green-2 (SPG2) are capable of detecting local concentration of H2O2 in subcellular compartments such as mitochondria, endoplasmic reticulum, nucleus, and plasma membrane. The precise localization of probes to the targeted organelle is facilitated by highly specific recognition of the SNAP ligand bound to the probe by the SNAP fusion protein. Furthermore, simultaneous detection of H2O2 at two different locations is feasible by using a SNAP tag with an orthogonal CLIP tag; such combined use of SNAP and CLIP tags is assisted by the expanding color palette of SNAP and CLIP peroxy probes. Multi-modal probes using PAMAM-G5 dendrimer platform was developed for the real-time imaging of the interplay between H2O2 and other physiological events. Coordination of the oxidative burst and progressive acidification in phagosomes of macrophages was elucidated with G5-SNARF2-PF1-Ac, a nanoprobe decorated with the H2O2 sensing module PF1 and pH sensor SNARF2.

Subjects/Keywords: Chemistry; Fluorescence Imaging; Hydrogen Peroxide

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Srikun, D. (2011). New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/4cj4q4xv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Srikun, Duangkhae. “New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells.” 2011. Thesis, University of California – Berkeley. Accessed January 21, 2021. http://www.escholarship.org/uc/item/4cj4q4xv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Srikun, Duangkhae. “New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells.” 2011. Web. 21 Jan 2021.

Vancouver:

Srikun D. New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2021 Jan 21]. Available from: http://www.escholarship.org/uc/item/4cj4q4xv.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Srikun D. New Chemical Tools for Fluorescent Detection of Hydrogen Peroxide in Living Cells. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/4cj4q4xv

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

4. Cooper, Justin T. Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces.

Degree: PhD, Chemistry, 2014, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3354/rec/2197

► The development of techniques to probe molecular transport and the dynamics of molecular interactions at interfaces is important for understanding and optimizingsurface-based technologies including surface-enhanced… (more)

▼ The development of techniques to probe molecular transport and the dynamics of molecular interactions at interfaces is important for understanding and optimizingsurface-based technologies including surface-enhanced spectroscopies, biological assays, sensors, catalysis, and chemical separations. In particular, the efficiency and resolution of separation via reversed-phase liquid chromatography is governed by the interaction ofanalytes with the solution/stationary phase interface. Most commonly, the stationary phase material consists of high surface area, micron-sized, mesoporous silica particles functionalized with n-alkane ligands. Understanding the timescales at which analytemolecules are transported through the interior of the particle, as well as adsorbed and desorbed from the particle surface, is of fundamental importance in the development of new, more efficient chromatographic materials.Probing chemical interactions at interfaces is difficult due to the selectivity needed to measure the small population of molecules at an interface versus bulk solution. Measuring interfacial chemical interactions within chromatographic particles has the added challenge that the majority of the surface area is contained within the particle making it difficult to measure interfacial processes directly.In this work, single-molecule spectroscopic techniques are used to measure the transport and adsorption/desorption kinetics of molecules at planar reversed-phase chromatographic interfaces and within reversed-phase chromatographic particles. Fluorescence imaging with single-molecule tracking is used to track the locations of fluorescent molecules during their retention within chromatographic particles. Thisyields information regarding their diffusion rates and their residence time within the particle. Statistical criteria based on the single-molecule localization resolution are also developed to characterize the population of strongly adsorbed molecules and their effect on intraparticle molecular residence times. Fluorescence imaging is also combined with fluorescence-correlation spectroscopy and used to measure fast interfacial transport and sorption kinetics at planar models of chromatographic interfaces. This technique has higher temporal resolutionrelative to imaging and is capable of measuring transport approaching free solution diffusion rates of small molecules.Finally, a comparison is made between interfacial transport rates and surface populations measured at planar chromatographic interfacial models versus within porous particles. It is found that n-alkyl modified planar interfaces are reasonable models for reversed-phase chromatographic particles with proper interpretation of measured parameters.

Subjects/Keywords: Fluorescence; Microscopy; Single-molecule imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cooper, J. T. (2014). Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces. (Doctoral Dissertation). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3354/rec/2197

Chicago Manual of Style (16^th Edition):

Cooper, Justin T. “Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces.” 2014. Doctoral Dissertation, University of Utah. Accessed January 21, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3354/rec/2197.

MLA Handbook (7^th Edition):

Cooper, Justin T. “Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces.” 2014. Web. 21 Jan 2021.

Vancouver:

Cooper JT. Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces. [Internet] [Doctoral dissertation]. University of Utah; 2014. [cited 2021 Jan 21]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3354/rec/2197.

Council of Science Editors:

Cooper JT. Single-molecule fluorescence microscopy of molecular interactions at reversed-phase chromatographic interfaces. [Doctoral Dissertation]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3354/rec/2197

Rice University

5. Quang, Timothy. Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia.

Degree: PhD, Engineering, 2016, Rice University

URL: http://hdl.handle.net/1911/95635

► Over the last decade, the five-year survival rate for oral cancer has remained at only 64%. Despite easy access to the oral cavity, most patients… (more)

▼ Over the last decade, the five-year survival rate for oral cancer has remained at only 64%. Despite easy access to the oral cavity, most patients with oral cancer are diagnosed at an advanced stage when treatment is more invasive and likely to be less successful. Imaging tools that can rapidly and accurately identify oral neoplasia could improve early detection of malignant oral lesions. This dissertation describes research to develop and evaluate a multi-modal optical imaging system with automated image processing to improve early detection of oral neoplasia. The multi-modal optical imaging system is comprised of two modalities, a high-resolution microendoscope (HRME) and a wide-field autofluorescence imager (AFI) to identify suspicious areas and to confirm whether suspicious areas contain neoplasia. A tablet-interfaced HRME with automated image analysis was developed and characterized to improve early detection of esophageal squamous cell carcinoma which has similar histologic patterns to oral neoplasia; results showed the tablet HRME can acquire comparable images to the first generation HRME design at a fraction of the cost and size. Training and validation was performed using a previously published dataset from a study of 177 patients referred for screening or surveillance endoscopy in China. Results showed that the automated image processing could differentiate between neoplastic and non-neoplastic images with a sensitivity of 95% and 91% in an independent validation set compared with 84% and 95% achieved in the original study. Additionally, automated image processing tools were developed to analyze wide-field autofluorescence images. The diagnostic performance of this approach was compared to previous results from a pilot study of 30 patients scheduled for surgical resection of a clinically suspicious oral lesion. The automated analysis method achieved a comparable area under the receiver operating characteristic curve (AUC) to the previous results based on manual analysis (0.862 automated vs. 0.877 manual) while minimizing dependence on user input. The automated analysis algorithms for AFI and HRME were then evaluated together to analyze images acquired from a population of 100 patients scheduled for surgical resection of a clinically suspicious oral lesion. A classification algorithm based on image metrics derived from AFI and HRME was able to correctly classify 100% of sites taken from biopsies pathologically diagnosed as normal and 85% of sites taken from biopsies diagnosed as moderate/severe dysplasia or cancer. These results provide evidence that multi-modal optical imaging with automated image analysis could be a valuable diagnostic adjunct for early detection of oral neoplasia. Advisors/Committee Members: Richards-Kortum, Rebecca (advisor).

Subjects/Keywords: optical imaging; oral cancer; fluorescence

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Quang, T. (2016). Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/95635

Chicago Manual of Style (16^th Edition):

Quang, Timothy. “Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia.” 2016. Doctoral Dissertation, Rice University. Accessed January 21, 2021. http://hdl.handle.net/1911/95635.

MLA Handbook (7^th Edition):

Quang, Timothy. “Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia.” 2016. Web. 21 Jan 2021.

Vancouver:

Quang T. Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia. [Internet] [Doctoral dissertation]. Rice University; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1911/95635.

Council of Science Editors:

Quang T. Development and Evaluation of a Multi-Modal Optical Imaging System for Early Identification of Oral Neoplasia. [Doctoral Dissertation]. Rice University; 2016. Available from: http://hdl.handle.net/1911/95635

University of Cambridge

6. Sims, Ruth Rebecca. Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/289719

► Quantitative three dimensional maps of cellular structure, activity and function provide the key to answering many prevalent questions in modern biological research. Fluorescence microscopy has… (more)

▼ Quantitative three dimensional maps of cellular structure, activity and function provide the key to answering many prevalent questions in modern biological research. Fluorescence microscopy has emerged as an indispensable tool in generating such maps, but common techniques are limited by fundamental physical constraints which render them incapable of simultaneously achieving high spatial and temporal resolution. This thesis will describe the development of novel microscopy techniques and complementary computational tools capable of addressing some of the aforementioned limitations of fluorescence microscopy and further outline their application in providing novel biological insights. The first section details the design of a light sheet microscope capable of high-throughput imaging of cleared, macroscopic samples with cellular resolution. In light sheet microscopy, the combination of spatially confined illumination with widefield detection enables multi-megapixel acquisition in a single camera exposure. The corresponding increase in acquisition speed enables systems level biological studies to be performed. The ability of this microscope to perform rapid, high-resolution imaging of intact samples is demonstrated by its application in a project which established a niche and hierarchy for stem cells in the adult nervous system. Light sheet microscopy achieves fast volumetric imaging rates, but the two dimensional nature of each measurement results in an inevitable lag between acquisition of the initial and final planes. The second section of this thesis describes the development and optimization of a light field microscope which captures volumetric information in a snapshot. Light field microscopy is a computational technique and images are reconstructed from raw data. Both the fidelity of computed volumes and the efficiency of the algorithms are strongly dependent on the quality of the rectification. A highly accurate, automated procedure is presented in this section. Light field reconstruction techniques are investigated and compared and the results are used to inform the re-design of the microscope. The new optical configuration is demonstrated to minimize the long-object problem. In the final section of the thesis, the spatial resolution limits of light field microscopy are explored using a combination of simulations and experiments. It is shown that light field microscopy is capable of localizing point sources over a large depth of field with high axial and lateral precision. Notably, this work paves the way towards frame rate limited super resolution localization microscopy with a depth of field larger than the thickness of a typical mammalian cell.

Subjects/Keywords: Fluorescence microscopy; Computational imaging; Volumetric imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sims, R. R. (2019). Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/289719

Chicago Manual of Style (16^th Edition):

Sims, Ruth Rebecca. “Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy.” 2019. Doctoral Dissertation, University of Cambridge. Accessed January 21, 2021. https://www.repository.cam.ac.uk/handle/1810/289719.

MLA Handbook (7^th Edition):

Sims, Ruth Rebecca. “Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy.” 2019. Web. 21 Jan 2021.

Vancouver:

Sims RR. Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Jan 21]. Available from: https://www.repository.cam.ac.uk/handle/1810/289719.

Council of Science Editors:

Sims RR. Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/289719

Georgia State University

7. Goolsby, Demesheka. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.

Degree: MS, Chemistry, 2016, Georgia State University

URL: https://scholarworks.gsu.edu/chemistry_theses/91

► Calcium (Ca²⁺), a crucial effector for many biological systems, has been associated with diseases such as cardiovascular disease, Alzheimer’s, Parkinson’s, cancer, and osteoporosis. It… (more)

Subjects/Keywords: Fluorescence; mCherry; EGFP; Calcium imaging; Fluorescence spectroscopy; Fluorescence lifetime

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goolsby, D. (2016). Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/chemistry_theses/91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goolsby, Demesheka. “Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.” 2016. Thesis, Georgia State University. Accessed January 21, 2021. https://scholarworks.gsu.edu/chemistry_theses/91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goolsby, Demesheka. “Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes.” 2016. Web. 21 Jan 2021.

Vancouver:

Goolsby D. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. [Internet] [Thesis]. Georgia State University; 2016. [cited 2021 Jan 21]. Available from: https://scholarworks.gsu.edu/chemistry_theses/91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goolsby D. Optical Property Enhancement And Characterization Of Fluorescent Protein Based Intracellular Calcium Probes. [Thesis]. Georgia State University; 2016. Available from: https://scholarworks.gsu.edu/chemistry_theses/91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oregon

8. Garrison, Zachary "Zach" Ryan. Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging.

Degree: 2020, University of Oregon

URL: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25748

► Biological imaging is an extremely important tool for biological and medical research. It allows researchers and doctors to access a perspective of biological systems not… (more)

Subjects/Keywords: Biochemistry; Chemistry; Organic Chemistry; Fluorescence Imaging; Flourophores; Imaging; Nanohoops; Cycloparaphenylene; Fluorescence

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garrison, Z. ". R. (2020). Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging. (Thesis). University of Oregon. Retrieved from https://scholarsbank.uoregon.edu/xmlui/handle/1794/25748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Garrison, Zachary "Zach" Ryan. “Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging.” 2020. Thesis, University of Oregon. Accessed January 21, 2021. https://scholarsbank.uoregon.edu/xmlui/handle/1794/25748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Garrison, Zachary "Zach" Ryan. “Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging.” 2020. Web. 21 Jan 2021.

Vancouver:

Garrison Z"R. Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging. [Internet] [Thesis]. University of Oregon; 2020. [cited 2021 Jan 21]. Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Garrison Z"R. Modification of Nanohoop Fluorophores to Improve Use in Biological Imaging. [Thesis]. University of Oregon; 2020. Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Indian Institute of Science

9. Ayyalasomayajula, Kalyan Ram. Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries.

Degree: MSc Engg, Faculty of Engineering, 2018, Indian Institute of Science

URL: http://etd.iisc.ac.in/handle/2005/3275

► New advances in computational modeling and instrumentation in the past decade has enabled the use of electromagnetic radiation for non-invasive monitoring of the physio-logical state… (more)

▼ New advances in computational modeling and instrumentation in the past decade has enabled the use of electromagnetic radiation for non-invasive monitoring of the physio-logical state of biological tissues. The near infrared (NIR) light having the wavelength range of 600 nm -1000 nm has been the main contender in these emerging molecular imaging modalities. Assessment of accurate pathological condition of the tissue under investigation relies on the contrast in the molecular images, where the endogenous contrast may not be suﬃcient in these scenarios. The ﬂuorescence (exogenous) contrast agents have been deployed to overcome these diﬃculties, where the preferential uptake by the tumor vasculature leads to high contrast,making this modality one of the biggest contenders in small-animal and soft-tissue molecular imaging modalities. In Fluorescence diﬀuse optical spectroscopy/imaging, this exogenous drug is excited by NIR laser light causing the emission of the ﬂuorescence light. The emitted ﬂuorescence light is typically dependent on the life time and concentration of the exogenous drug coupled with physiology associated with the tissue under investigation. As there is an excitation and emission of the light,the underlying physics of the problem is described by a coupled diﬀusion equations. These coupled diﬀusion equations are typically solved by advanced numerical methods, which tend to be computationally demanding. In this work, analytical solutions for these coupled partial diﬀerential equations (PDEs) for the regular geometries for both time-domain and frequency-domain cases were developed. Till now, the existing literature has not dealt with all regular geometries and derived analytical solutions were only for couple of geometries. Here a universally acceptable generic solution was developed based on Green’s function approach that is applicable to any regular geometry. Using this, the analytical solutions for the regular geometries that is encountered in diﬀuse ﬂuorescence spectroscopy/imaging were obtained. These solutions can play an important role in determining the bulk ﬂuorescence properties of the tissue, which could act as good initial guesses for the advanced image reconstruction techniques and/or can also facilitate the calibration of experimental ﬂuorescence data by removing biases and source-detector variations. In the second part of this work, the developed analytical models for regular geometries were validated through comparison with the established numerical models that are traditionally used in the diﬀuse ﬂuorescence spectroscopy/imaging. This comparison not only validated the developed analytical models, but also showed that analytical models are capable of providing bulk ﬂuorescence properties with at least one order of magnitude less computational cost compared to the highly optimized traditional numerical models. Advisors/Committee Members: Yalavarthy, Phaneendra K (advisor).

Subjects/Keywords: Medical Optics; Medical Imaging; Diffuse Fluorescence Spectroscopy; Fluorescence Diffuse Optical Imaging; Fluorescence Diffuse Optical Spectroscopy; Diffuse Fluorescence Spectroscopy/Imaging; Fluorescence Spectroscopy/Imaging - Mathematical Models; Molecular Imaging; Fouorescence Spectroscopy/Imaging; Fluorescence Optical Breast Imaging; Flrorescence Optical Brain Imaging; Fluorescence Diffuse Optical Imaging; FDOI; Biotechnology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ayyalasomayajula, K. R. (2018). Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries. (Masters Thesis). Indian Institute of Science. Retrieved from http://etd.iisc.ac.in/handle/2005/3275

Chicago Manual of Style (16^th Edition):

Ayyalasomayajula, Kalyan Ram. “Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries.” 2018. Masters Thesis, Indian Institute of Science. Accessed January 21, 2021. http://etd.iisc.ac.in/handle/2005/3275.

MLA Handbook (7^th Edition):

Ayyalasomayajula, Kalyan Ram. “Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries.” 2018. Web. 21 Jan 2021.

Vancouver:

Ayyalasomayajula KR. Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries. [Internet] [Masters thesis]. Indian Institute of Science; 2018. [cited 2021 Jan 21]. Available from: http://etd.iisc.ac.in/handle/2005/3275.

Council of Science Editors:

Ayyalasomayajula KR. Development and Validation of Analytical Models for Diffuse Fluorescence Spectroscopy/Imaging in Regular Geometries. [Masters Thesis]. Indian Institute of Science; 2018. Available from: http://etd.iisc.ac.in/handle/2005/3275

University of Texas – Austin

10. -4953-3644. Optical and structural property mapping of soft tissues using spatial frequency domain imaging.

Degree: PhD, Biomedical engineering, 2015, University of Texas – Austin

URL: http://hdl.handle.net/2152/31345

► Tissue optical properties, absorption, scattering and fluorescence, reveal important information about health, and holds the potential for non-invasive diagnosis and therefore earlier treatment for many… (more)

▼ Tissue optical properties, absorption, scattering and fluorescence, reveal important information about health, and holds the potential for non-invasive diagnosis and therefore earlier treatment for many diseases. On the other hand, tissue structure determines its function. Studying tissue structural properties helps us better understand structure-function relationship. Optical imaging is an ideal tool to study these tissue properties. However, conventional optical imaging techniques have limitations, such as not being able to quantitatively evaluate tissue absorption and scattering properties and only providing volumetrically averaged quantities with no depth control capability. To better study tissue properties, we integrated spatial frequency domain imaging (SFDI) with conventional reflectance imaging modalities. SFDI is a non-invasive, non-contact wide-field imaging technique which utilizes structured illumination to probe tissues. SFDI imaging is able to accurately quantify tissue optical properties. By adjusting spatial frequency, the imaging depth can be tuned which allows for depth controlled imaging. Especially at high spatial frequency, SFDI reflectance image is more sensitive to tissue scattering property than absorption property. The imaging capability of SFDI allows for studying tissue properties from a whole new perspective. In our study, we developed both benchtop and handheld SFDI imaging systems to accommodate different applications. By evaluating tissue optical properties, we corrected attenuation in fluorescence imaging using an analytical model; and we quantified optical and physical properties of skin diseases. By imaging at high spatial frequency, we demonstrated that absorption in fluorescence imaging can also be reduced because of a reduced imaging depth. This correction can be performed in real-time at 19 frames/second. Furthermore, fibrous structures orientation from the superficial layer can be accurately quantified in a multi-layered sample by limiting imaging depth. Finally, we color rendered SFDI reflectance image at high spatial frequency to reveal structural changes in skin lesions. Advisors/Committee Members: Tunnell, James W. (advisor), Krishnan, Sunil (committee member), Reichenberg, Jason S (committee member), Yeh, Hsin-Chih (committee member), Sacks, Michael (committee member).

Subjects/Keywords: Spatial frequency domain imaging; Fluorescence imaging; Polarized light imaging; Hyperspectral imaging; Imaging instrument

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

-4953-3644. (2015). Optical and structural property mapping of soft tissues using spatial frequency domain imaging. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/31345

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

-4953-3644. “Optical and structural property mapping of soft tissues using spatial frequency domain imaging.” 2015. Doctoral Dissertation, University of Texas – Austin. Accessed January 21, 2021. http://hdl.handle.net/2152/31345.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

-4953-3644. “Optical and structural property mapping of soft tissues using spatial frequency domain imaging.” 2015. Web. 21 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

-4953-3644. Optical and structural property mapping of soft tissues using spatial frequency domain imaging. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2152/31345.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

-4953-3644. Optical and structural property mapping of soft tissues using spatial frequency domain imaging. [Doctoral Dissertation]. University of Texas – Austin; 2015. Available from: http://hdl.handle.net/2152/31345

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Washington University in St. Louis

11. Gao, Shengkui. Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery.

Degree: PhD, Computer Science & Engineering, 2015, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/118

► Image guided surgery (IGS) utilizes emerging imaging technologies to provide additional structural and functional information to the physician in clinical settings. This additional visual… (more)

Subjects/Keywords: Imaging Sensor; Imaging System; Multi-spectral Imaging; NIR fluorescence; Polarization; Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, S. (2015). Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/118

Chicago Manual of Style (16^th Edition):

Gao, Shengkui. “Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery.” 2015. Doctoral Dissertation, Washington University in St. Louis. Accessed January 21, 2021. https://openscholarship.wustl.edu/eng_etds/118.

MLA Handbook (7^th Edition):

Gao, Shengkui. “Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery.” 2015. Web. 21 Jan 2021.

Vancouver:

Gao S. Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2015. [cited 2021 Jan 21]. Available from: https://openscholarship.wustl.edu/eng_etds/118.

Council of Science Editors:

Gao S. Bio-Inspired Multi-Spectral Imaging Sensors and Algorithms for Image Guided Surgery. [Doctoral Dissertation]. Washington University in St. Louis; 2015. Available from: https://openscholarship.wustl.edu/eng_etds/118

University of California – Berkeley

12. Van de Bittner, Genevieve Crystal. Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease.

Degree: Chemistry, 2012, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/76v708pp

► The study of biological processes involved in the development and progression of disease has the potential to uncover new treatments or cures. While there are… (more)

▼ The study of biological processes involved in the development and progression of disease has the potential to uncover new treatments or cures. While there are many techniques to study biological analytes involved in disease states, molecular imaging offers tools that are amenable to imaging biological processes as they occur in living systems. Previously developed fluorescent molecular imaging tools have lead to an improved understanding of the roles of both hydrogen peroxide (H₂O₂) and copper in both normal physiological processes and in disease. However, most of these tools have been limited to the study of H₂O₂ and copper in cells or tissues. A more comprehensive understanding of molecular disease processes involved in disease states can be gleaned by studying animal models of human disease, as the affects of the disease on the entire organism can be monitored. This dissertation describes the design, synthesis, and characterization of bioluminescent and fluorescent molecular imaging tools for the detection of H₂O₂ and copper, and their application to the detection of these analytes in murine models of disease. In a first demonstration, Peroxy Caged Luciferin-1 is developed using a firefly luciferin probe scaffold and utilized to monitor increased H₂O₂ production in androgen-sensitive prostate tumors following stimulation with a growth-inducing compound, testosterone. Using a new approach for firefly luminescence imaging, in situ formation of firefly luciferin, two unique probes, Peroxy Caged Luciferin-2 and IETDC, are developed for simultaneous imaging of H₂O₂ and caspase 8 activity in a model of sepsis. Development of a third luminescence-based probe for H₂O₂ detection, Peroxy Caged Luciferin-3, offers a tool for the detection of H₂O₂ in the brain of mice, with possible applications for studying H₂O₂ during seizure. Finally, development of a near-infrared fluorescent probe for detection of copper in vivo is discussed and applied to the detection of alterations in copper levels during the development and treatment of a murine Wilson's disease model.

Subjects/Keywords: Chemistry; bioluminescence; copper; fluorescence; hydrogen peroxide; imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van de Bittner, G. C. (2012). Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/76v708pp

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van de Bittner, Genevieve Crystal. “Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease.” 2012. Thesis, University of California – Berkeley. Accessed January 21, 2021. http://www.escholarship.org/uc/item/76v708pp.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van de Bittner, Genevieve Crystal. “Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease.” 2012. Web. 21 Jan 2021.

Vancouver:

Van de Bittner GC. Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease. [Internet] [Thesis]. University of California – Berkeley; 2012. [cited 2021 Jan 21]. Available from: http://www.escholarship.org/uc/item/76v708pp.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van de Bittner GC. Molecular Imaging Approaches toward Optical Detection of Hydrogen Peroxide and Copper in Murine Models of Disease. [Thesis]. University of California – Berkeley; 2012. Available from: http://www.escholarship.org/uc/item/76v708pp

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

13. Wang, Ying-ting. Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules.

Degree: Master, Chemistry, 2018, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0003118-150231

► In recent years, noble metal nanoclusters have been extensively studied, while the research on copper nanoclusters is relatively rare. Gold, silver, and copper are contained… (more)

▼ In recent years, noble metal nanoclusters have been extensively studied, while the research on copper nanoclusters is relatively rare. Gold, silver, and copper are contained in the periodic table of the same group of elements. Copper is Earth-abundant, low prices and relatively friendly to humans. In this study, we direct use of pepsin as a template, so that with high biocompatibility and without additional modification. And we also use low toxicity reducing agents such as NaBH4 and ascorbic acid in the alkaline environment. Then, copper nanocluster is successfully synthesized with the excitation wavelength at 420 nm and the emission wavelength at 496 nm. It is deduced that Cu13 is the main product and uses its characteristics as a fluorescent probe. In the process, copper nanoclusters and hemoglobin were used as an IFE absorber/fluorophore pair and can be successfully used in real urine samples hemoglobin detection. This method is convenient, the detection limit of 70 nM. In addition, in different acid-base environments, the pepsin changes its structure due to the protonation and deprotonation of functional groups, resulting in a change in its fluorescence intensity. It can be used as a fluorescent probe to detect the change of pH value in the environment. The experimental results can be obtained in the linear range of pH 2.0 ~ pH 6.5, which can distinguish the difference of pH range of 0.5. After repeated regulation in the acid-base environment, they still keep high stability. After that, copper nanoclusters are used in the detection of urea, detection limit of 0.1mM, and can be successfully applied to the human urine sample. Finally, using laser scanning confocal microscopy to observe the copper nanoclusters, the fluorescence intensity of the copper nanoclusters showed an enhanced trend between pH 4.0 and pH 6.0, indicating that the copper nanoclusters have high cell penetration and high biocompatibility. Advisors/Committee Members: Cheng-Ju Yu (chair), Tai-Chia Chiu (chair), Wei-Lung Tseng (committee member).

Subjects/Keywords: fluorescence imaging; urea; hemoglobin; pH; copper nanoclusters

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2018). Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0003118-150231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Ying-ting. “Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules.” 2018. Thesis, NSYSU. Accessed January 21, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0003118-150231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Ying-ting. “Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules.” 2018. Web. 21 Jan 2021.

Vancouver:

Wang Y. Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules. [Internet] [Thesis]. NSYSU; 2018. [cited 2021 Jan 21]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0003118-150231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang Y. Protein-directed synthesis of luminescent copper nanoclusters for sensing bio-molecules. [Thesis]. NSYSU; 2018. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0003118-150231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

14. George, Jonathan. A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles.

Degree: PhD, Nuclear, Plasma, Radiolgc Engr, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/101107

► X-ray-activated photodynamic therapy (X-PDT) techniques have gained traction for its potential to impart therapeutic effects at greater depths than possible with traditional photodynamic therapy [1],… (more)

Subjects/Keywords: X-ray; Nanoparticle; Fluorescence; Luminescence; Imaging

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

George, J. (2017). A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101107

Chicago Manual of Style (16^th Edition):

George, Jonathan. “A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles.” 2017. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 21, 2021. http://hdl.handle.net/2142/101107.

MLA Handbook (7^th Edition):

George, Jonathan. “A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles.” 2017. Web. 21 Jan 2021.

Vancouver:

George J. A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2142/101107.

Council of Science Editors:

George J. A tri-modality x-ray fluorescence, x-ray luminescence, x-ray transmission computed tomography imaging platform for monitoring and stimulating metal-containing nanoparticles. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/101107

Texas A&M University

15. Bixler, Joel Nathan. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.

Degree: MS, Biomedical Engineering, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153844

► We have integrated a fluorescence microendoscope into a whole-animal optical imaging system, allowing for simultaneous microscopic and macroscopic imaging of tdTomato expressing BCG in vivo.… (more)

Subjects/Keywords: Fluorescence spectroscopy; Bacterial Imaging; Tuberculosis; microendoscope

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bixler, J. N. (2014). Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153844

Chicago Manual of Style (16^th Edition):

Bixler, Joel Nathan. “Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.” 2014. Masters Thesis, Texas A&M University. Accessed January 21, 2021. http://hdl.handle.net/1969.1/153844.

MLA Handbook (7^th Edition):

Bixler, Joel Nathan. “Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging.” 2014. Web. 21 Jan 2021.

Vancouver:

Bixler JN. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1969.1/153844.

Council of Science Editors:

Bixler JN. Multi-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imaging. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153844

University of Connecticut

16. Longo, Kaitlyn M. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.

Degree: MS, Biomedical Engineering, 2014, University of Connecticut

URL: https://opencommons.uconn.edu/gs_theses/594

► Otitis Media is defined as inflammation of the middle ear region associated with middle ear fluid. This condition typically follows an upper respiratory infection,… (more)

▼ Otitis Media is defined as inflammation of the middle ear region associated with middle ear fluid. This condition typically follows an upper respiratory infection, is commonly misdiagnosed by physicians and can potentially lead to the inappropriate use of antimicrobial agents. ^[1] This thesis describes the design of a novel medical device that utilized spectroscopy and digital imaging to provide physicians with a unique representation of the middle ear. Published literature has been reviewed to assess the different approaches that researchers have studied to improve the diagnosis of otitis media, where, for example, research conducted by Thorton et al. and Boppart et al. used fluorescence to identify bacteria contained in biofilms.^[12,13] In addition, a population study conducted by Jensen et al. determined what the level of diagnostic certainty is in pediatric patients and determined that general practitioners had a diagnostic certainty of 58% in patients under one year of age and 73% certainty in patients over two and a half years of age.^[9] Eleven subjects, each with a history of recurrent acute otitis media and requiring myringotomy and ear tube placement, were selected for study in this thesis. All subjects received a digital imaging and spectroscopy examination using the device at the time of surgery. Five excitation and illumination wavelengths were used, including white light, 385-420 nm, 450-470 nm, 520-535 nm, and 640-650 nm as well as fluorescence imaging using 425 nm and 450 nm filters. Each of the chosen wavelengths were emitted into the ear canal and tympanic membrane during a typical otoscopic examination. Signal processing techniques were also applied to isolate various regions of the tympanic membrane using MatLAB to increase the resolution of the anatomical and physiological properties of the tympanic membrane. The spectral readings between the wavelengths of 560 nm and 760 nm were plotted for each subject and were classified according to fluid retention, consistency, and vascularity. In this subject population, eight of the 22 ears were identified to be a noneffusion normal. Reflectance of keratin, cerumen, and bone, with well defined blood vessels and contrast, allowed for better characterization of the middle ear. ^[8] Reflectance spectra were classified according to fluid consistency and trends were determined for non effusion and effusion patients. Class one spectra were defined as tympanic membrane and canal spectra with a rapid increase in percent reflection between 575 nm and 650 nm and a rapid decrease in percent reflection between 650 nm and 750 nm. Class two spectra were defined as tympanic membrane and canal spectra with a gradual decrease in percent reflection between 575 nm and 800 nm. This novel device was successfully used to detect effusion consistency and color and it will continue to aid in the development of a new diagnostic procedure. It provides a means for the creation of a new diagnostic procedure that may be useful… Advisors/Committee Members: Tulio Valdez, Martin Cherniack, Donald Peterson.

Subjects/Keywords: Otitis Media; Otolaryngology; Digital Imaging; Fluorescence; Spectroscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Longo, K. M. (2014). Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/594

Chicago Manual of Style (16^th Edition):

Longo, Kaitlyn M. “Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.” 2014. Masters Thesis, University of Connecticut. Accessed January 21, 2021. https://opencommons.uconn.edu/gs_theses/594.

MLA Handbook (7^th Edition):

Longo, Kaitlyn M. “Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis.” 2014. Web. 21 Jan 2021.

Vancouver:

Longo KM. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. [Internet] [Masters thesis]. University of Connecticut; 2014. [cited 2021 Jan 21]. Available from: https://opencommons.uconn.edu/gs_theses/594.

Council of Science Editors:

Longo KM. Bimodal Approach Using Spectroscopy and Digitial Imaging to Assist Otitis Media Diagnosis. [Masters Thesis]. University of Connecticut; 2014. Available from: https://opencommons.uconn.edu/gs_theses/594

University of Toronto

17. Downie, Kelsey Jean. Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding.

Degree: 2013, University of Toronto

URL: http://hdl.handle.net/1807/42829

►

The carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) is a human receptor that facilitates adhesion with neighbouring cells, as well as with certain pathogens. CEACAM1… (more)

Subjects/Keywords: fluorescence microscopy; CEACAM; live cell imaging; 0541

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Downie, K. J. (2013). Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/42829

Chicago Manual of Style (16^th Edition):

Downie, Kelsey Jean. “Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding.” 2013. Masters Thesis, University of Toronto. Accessed January 21, 2021. http://hdl.handle.net/1807/42829.

MLA Handbook (7^th Edition):

Downie, Kelsey Jean. “Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding.” 2013. Web. 21 Jan 2021.

Vancouver:

Downie KJ. Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1807/42829.

Council of Science Editors:

Downie KJ. Live Cell Imaging of CEACAM1 Dynamics and Self-association during Bacterial Binding. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/42829

Columbia University

18. Galwaduge, Pubudu Thilanka. Optimization of two-photon excited fluorescence for volumetric imaging.

Degree: 2017, Columbia University

URL: https://doi.org/10.7916/D8MW2VN6

► Two-photon microscopy is often used in biological imaging due to its optical sectioning and depth penetration capabilities. These characteristics have made two-photon microscopy especially useful… (more)

▼ Two-photon microscopy is often used in biological imaging due to its optical sectioning and depth penetration capabilities. These characteristics have made two-photon microscopy especially useful for neurobiological studies where imaging a volume at single cell resolution is typically required. This dissertation focuses on the optimization of two-photon excited fluorescence for volumetric imaging of biological samples, with special attention to imaging the mouse brain. Chapter 2 studies wavefront manipulation as a way of optimizing two-photon excited fluorescence. We show, through numerical simulations and experiments, that the magnitude of the two-photon fluorescence signal originating from cell-sized objects can be used as a metric of beam quality. We also show that the cranial window used in mouse experiment is a major source of aberrations, which can readily be represented in the Zernike basis. Finally, we implement a modal wavefront optimization scheme that optimizes the wavefront based entirely on the magnitude of the fluorescence. Along with this scheme, Zernike functions are found to be a useful basis for correcting aberrations encountered in mouse brain imaging while the Hadamard basis is found to be useful for scattering compensation. Corrections performed in mouse brain using Zernike functions are found to be valid over hundreds of microns, allowing a single correction to be applied to a whole volume. Finally, we show that the wavefront correction system can double as a wavefront encoding system for experiments that require custom point-spread-functions. Chapter 3 aims to significantly improve the volume imaging rate of two-photon microscopy. The imaging speed is improved by combining two-photon excitation with scanning confocally-aligned planar excitation microscopy (SCAPE). Numerical simulations, analytical arguments, and experiments reveal that the standard method of combining nano-joule pulses with 80 MHz repetition rates is inadequate for two-photon light-sheet excitation. We use numerical simulations and experiments to explore the possibility of achieving fast volumetric imaging using line and sheet excitation and find that the sheet excitation scheme is more promising. Given that two-photon excitation requires high photon-flux-densities near the focus, achieving high enough fluorescence has to be balanced with restrictions placed by saturation, photodamage, photobleaching and sample heating effects. Finally, we experimentally study light sheet excitation at various pulse repetition rates with femtosecond pulses and find that repetition rates near 100 kHz allow imaging of nonbiological samples of ~200x300x300 μm³ volume at 20 volumes per second while balancing the above constraints. This work paves the way for achieving fast, volumetric two-photon imaging of the mouse brain.

Subjects/Keywords: Imaging systems in biology; Fluorescence microscopy; Physics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Galwaduge, P. T. (2017). Optimization of two-photon excited fluorescence for volumetric imaging. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8MW2VN6

Chicago Manual of Style (16^th Edition):

Galwaduge, Pubudu Thilanka. “Optimization of two-photon excited fluorescence for volumetric imaging.” 2017. Doctoral Dissertation, Columbia University. Accessed January 21, 2021. https://doi.org/10.7916/D8MW2VN6.

MLA Handbook (7^th Edition):

Galwaduge, Pubudu Thilanka. “Optimization of two-photon excited fluorescence for volumetric imaging.” 2017. Web. 21 Jan 2021.

Vancouver:

Galwaduge PT. Optimization of two-photon excited fluorescence for volumetric imaging. [Internet] [Doctoral dissertation]. Columbia University; 2017. [cited 2021 Jan 21]. Available from: https://doi.org/10.7916/D8MW2VN6.

Council of Science Editors:

Galwaduge PT. Optimization of two-photon excited fluorescence for volumetric imaging. [Doctoral Dissertation]. Columbia University; 2017. Available from: https://doi.org/10.7916/D8MW2VN6

Hong Kong University of Science and Technology

19. Yu, Lujia SENG. Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels.

Degree: 2019, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-100093 ; https://doi.org/10.14711/thesis-991012730761603412 ; http://repository.ust.hk/ir/bitstream/1783.1-100093/1/th_redirect.html

► Stochastic Optical Reconstruction Microscopy (STORM) is proved to be one of greatest tools for medical and biological research which can provide images with resolution beyond… (more)

Subjects/Keywords: High resolution imaging ; DNA ; Analysis ; Fluorescence microscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, L. S. (2019). Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-100093 ; https://doi.org/10.14711/thesis-991012730761603412 ; http://repository.ust.hk/ir/bitstream/1783.1-100093/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yu, Lujia SENG. “Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels.” 2019. Thesis, Hong Kong University of Science and Technology. Accessed January 21, 2021. http://repository.ust.hk/ir/Record/1783.1-100093 ; https://doi.org/10.14711/thesis-991012730761603412 ; http://repository.ust.hk/ir/bitstream/1783.1-100093/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yu, Lujia SENG. “Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels.” 2019. Web. 21 Jan 2021.

Vancouver:

Yu LS. Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2019. [cited 2021 Jan 21]. Available from: http://repository.ust.hk/ir/Record/1783.1-100093 ; https://doi.org/10.14711/thesis-991012730761603412 ; http://repository.ust.hk/ir/bitstream/1783.1-100093/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yu LS. Three color super-resolution localization microscopy for optical mapping of stretched DNAs in nanochannels. [Thesis]. Hong Kong University of Science and Technology; 2019. Available from: http://repository.ust.hk/ir/Record/1783.1-100093 ; https://doi.org/10.14711/thesis-991012730761603412 ; http://repository.ust.hk/ir/bitstream/1783.1-100093/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan Technological University

20. Fedie, Joseph. Fluorescent Probe Development for Fructose Specific Transporters in Cancer.

Degree: MS, Department of Chemistry, 2017, Michigan Technological University

URL: https://digitalcommons.mtu.edu/etdr/332

► Carbohydrate transporters or GLUTs of the major facilitator superfamily (MFS) are responsible for transporting sugars into the cell and have been of research interest… (more)

Subjects/Keywords: GLUTs; Cancer; Chemical Biology; Imaging; Fluorescence; Therapeutics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fedie, J. (2017). Fluorescent Probe Development for Fructose Specific Transporters in Cancer. (Masters Thesis). Michigan Technological University. Retrieved from https://digitalcommons.mtu.edu/etdr/332

Chicago Manual of Style (16^th Edition):

Fedie, Joseph. “Fluorescent Probe Development for Fructose Specific Transporters in Cancer.” 2017. Masters Thesis, Michigan Technological University. Accessed January 21, 2021. https://digitalcommons.mtu.edu/etdr/332.

MLA Handbook (7^th Edition):

Fedie, Joseph. “Fluorescent Probe Development for Fructose Specific Transporters in Cancer.” 2017. Web. 21 Jan 2021.

Vancouver:

Fedie J. Fluorescent Probe Development for Fructose Specific Transporters in Cancer. [Internet] [Masters thesis]. Michigan Technological University; 2017. [cited 2021 Jan 21]. Available from: https://digitalcommons.mtu.edu/etdr/332.

Council of Science Editors:

Fedie J. Fluorescent Probe Development for Fructose Specific Transporters in Cancer. [Masters Thesis]. Michigan Technological University; 2017. Available from: https://digitalcommons.mtu.edu/etdr/332

University of Missouri – Columbia

21. Cavins, Patrick L. Fluorine-18 capture by substituted BODIPY derivatives.

Degree: 2015, University of Missouri – Columbia

URL: http://hdl.handle.net/10355/46845

► For the medical community to effectively decrease the number of deaths caused by cancer each year, we must develop tools that allow for the early… (more)

Subjects/Keywords: Diagnostic imaging; Radiochemistry; Fluorescence; Cancer – Early detection

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cavins, P. L. (2015). Fluorine-18 capture by substituted BODIPY derivatives. (Thesis). University of Missouri – Columbia. Retrieved from http://hdl.handle.net/10355/46845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cavins, Patrick L. “Fluorine-18 capture by substituted BODIPY derivatives.” 2015. Thesis, University of Missouri – Columbia. Accessed January 21, 2021. http://hdl.handle.net/10355/46845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cavins, Patrick L. “Fluorine-18 capture by substituted BODIPY derivatives.” 2015. Web. 21 Jan 2021.

Vancouver:

Cavins PL. Fluorine-18 capture by substituted BODIPY derivatives. [Internet] [Thesis]. University of Missouri – Columbia; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10355/46845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cavins PL. Fluorine-18 capture by substituted BODIPY derivatives. [Thesis]. University of Missouri – Columbia; 2015. Available from: http://hdl.handle.net/10355/46845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Washington University in St. Louis

22. Wang, Zhenghui. Studies of fluorescent imaging for mRNA detection in living cells.

Degree: PhD, Chemistry, 2011, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/etd/663

► This dissertation focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of… (more)

▼ This dissertation focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) mRNA by strand-displacement activated Peptide Nucleic Acid: PNA) probes.: 3) Imaging of iNOS mRNA by binary fluorescently labeled PNA probes. The first approach was based on the work of our former lab member Dr. Huafeng Fang, who had constructed a multiple MG binding aptamer tagged transgene: Flag-mβ2AR-GFP-MGVI), which could also express a green fluorescence protein associated with an adrenergic receptor protein. It has been reported that the tagged aptamer sequence can increase the fluorescence of MG up to 2000 fold by binding to MG. Total RNA extract of the transfected MDCK cells has shown up to 22 times increase of fluorescence in the presence of MG. Confocal fluorescence imaging study has shown that in the presence of MG, cells expressing the transgene showed both the fluorescence of GFP and enhanced fluorescence of MG. A flow cytometry study detected that in the presence of MG and transfected cells showed 1.3 fold increase of fluorescence compared to the wild type MDCK cells. The next approach was to use strand-displacement activated PNA probes to detect the iNOS mRNA in living RAW 264.7 mouse macrophage cells. A probe constitutes of an antisense 23-mer fluorescein: FAM) labeled antisense PNA and a 17-mer Dabcylplus labeled complementary DNA was used. The fluorescence of the FAM was quenched when the two strands hybridized to each other. In the presence of target mRNA, the shorter strand was displaced by the mRNA, which has more base pairs complementary to the PNA. The fluorescence of FAM was restored and thus could be used to detect the mRNA. The probe has been shown to be able to detect the target DNA and in vitro transcribed mRNA in solution. Fluorescence in situ hybridization: FISH) showed that the probes showed 3.6: Â± 1.8)-fold increase of fluorescence between stimulated cells expressing a high level of iNOS mRNA and non-stimulated cells. Cationic Shell-crosslinked Knedel-like: cSCK) nanoparticles were employed to deliver probes into living cells and the fluorescence of the stimulated cells observed by confocal microscopy increased 16.6: Â± 7.9)-fold. RT-PCR was conducted to determine the absolute copy number of the iNOS mRNA in cells. The detected increase of iNOS mRNA after 18 hours of stimulation was around 100 times, and the actual copy number of the mRNA per cell was around 70000. These results reveal that the under our current systems, strand-displacement probes are not sufficient to report quantitatively on the mRNA copy numbers in living cells, but they can be used to achieve mRNA imaging in living cells. The third approach was to use fluorescent labeled binary PNA probes to image the iNOS mRNA in living RAW 264.7 cells. PNAs bearing FAM and Cy5 and targeting the adjacent sites of the iNOS mRNA… Advisors/Committee Members: John-Stephen Taylor.

Subjects/Keywords: Chemistry; Biochemistry; Fluorescence; Imaging; mRNA; PNA

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Z. (2011). Studies of fluorescent imaging for mRNA detection in living cells. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/663

Chicago Manual of Style (16^th Edition):

Wang, Zhenghui. “Studies of fluorescent imaging for mRNA detection in living cells.” 2011. Doctoral Dissertation, Washington University in St. Louis. Accessed January 21, 2021. https://openscholarship.wustl.edu/etd/663.

MLA Handbook (7^th Edition):

Wang, Zhenghui. “Studies of fluorescent imaging for mRNA detection in living cells.” 2011. Web. 21 Jan 2021.

Vancouver:

Wang Z. Studies of fluorescent imaging for mRNA detection in living cells. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2011. [cited 2021 Jan 21]. Available from: https://openscholarship.wustl.edu/etd/663.

Council of Science Editors:

Wang Z. Studies of fluorescent imaging for mRNA detection in living cells. [Doctoral Dissertation]. Washington University in St. Louis; 2011. Available from: https://openscholarship.wustl.edu/etd/663

Washington University in St. Louis

23. Maji, Dolonchampa. Development and applications of novel fluorescent molecular probe strategies.

Degree: PhD, Biomedical Engineering, 2017, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/eng_etds/242

► Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and… (more)

▼ Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging. The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence. Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo. An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. … Advisors/Committee Members: Samuel Achilefu, Mark Anastasio, Gregory M. Lanza, Srikanth Singamaneni, Lihong V. Wang.

Subjects/Keywords: contrast agent; fluorescence; optical imaging; Biomedical

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Maji, D. (2017). Development and applications of novel fluorescent molecular probe strategies. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/242

Chicago Manual of Style (16^th Edition):

Maji, Dolonchampa. “Development and applications of novel fluorescent molecular probe strategies.” 2017. Doctoral Dissertation, Washington University in St. Louis. Accessed January 21, 2021. https://openscholarship.wustl.edu/eng_etds/242.

MLA Handbook (7^th Edition):

Maji, Dolonchampa. “Development and applications of novel fluorescent molecular probe strategies.” 2017. Web. 21 Jan 2021.

Vancouver:

Maji D. Development and applications of novel fluorescent molecular probe strategies. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2017. [cited 2021 Jan 21]. Available from: https://openscholarship.wustl.edu/eng_etds/242.

Council of Science Editors:

Maji D. Development and applications of novel fluorescent molecular probe strategies. [Doctoral Dissertation]. Washington University in St. Louis; 2017. Available from: https://openscholarship.wustl.edu/eng_etds/242

University of Arizona

24. Uthoff, Ross. Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer .

Degree: 2015, University of Arizona

URL: http://hdl.handle.net/10150/593619

► Shown is the design of the Smartphone Oral Cancer Detection System (SOCeeDS). The SOCeeDS attaches to a smartphone and utilizes its embedded imaging optics and… (more)

Subjects/Keywords: illumination; imaging; mHealth; optics; Optical Sciences; fluorescence

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Uthoff, R. (2015). Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/593619

Chicago Manual of Style (16^th Edition):

Uthoff, Ross. “Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer .” 2015. Masters Thesis, University of Arizona. Accessed January 21, 2021. http://hdl.handle.net/10150/593619.

MLA Handbook (7^th Edition):

Uthoff, Ross. “Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer .” 2015. Web. 21 Jan 2021.

Vancouver:

Uthoff R. Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer . [Internet] [Masters thesis]. University of Arizona; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10150/593619.

Council of Science Editors:

Uthoff R. Design of a Smartphone-Camera-based Fluorescence Imaging System for the Detection of Oral Cancer . [Masters Thesis]. University of Arizona; 2015. Available from: http://hdl.handle.net/10150/593619

McMaster University

25. Hirmiz, Nehad. DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS.

Degree: 2019, McMaster University

URL: http://hdl.handle.net/11375/25137

►

Protein-protein interactions are important for biological processes. Therefore, many small molecules target a specific protein or interaction in the cell to have biological consequence. While… (more)

▼

Protein-protein interactions are important for biological processes. Therefore, many small molecules target a specific protein or interaction in the cell to have biological consequence. While we can measure some protein-protein interactions in a test tube, many proteins cannot be purified making it difficult to properly test that a drug is “on target”. An alternative is to measure these interactions in live cells. We express the proteins of interest fused to fluorophores allowing the use of fluorescence techniques. Förster Resonance Energy Transfer (FRET) provides a molecular level ruler to measure the distance, within a few nanometers, between two proteins. FRET indicates binding. The gold standard for measuring FRET in live cells is by quantifying changes in fluorescence lifetime using Fluorescence lifetime imaging microscopy (FLIM). The change in fluorescence lifetime is inversely proportional to the ratio of bound to non-bound proteins. Tradition FLIM-FRET microscopy is too slow for screening applications. Our aim was to develop a highly multiplexed confocal system for rapid FLIM-FRET acquisition. We present the development of multiple prototypes for confocal multiplexing. In this work, our final design includes 32×32 multiplexed excitation points which scan the sample using refractive window scanners. We coupled this excitation scheme to a 64×32 time-gated single-photon avalanche photodiode (SPAD) sparse array detector. This multiplexed setup allows the use of the sparse array with high frame rate and sub-nanosecond time-gating to achieve high throughput FLIM acquisition. Using our multiplexed FLIM prototype we measured Bcl-2 family protein-protein interactions in live cells (310×310 μm FOV) with two-channel confocal FLIM in 1.5 s. Protein binding affinities were estimated by measuring the changes in FRET as a function of acceptor to donor ratio. The resulting speed of this system meets requirements for implementation in screening applications.

Thesis

Candidate in Philosophy

Inside a cell, proteins are the “workers” and they interact with each other, doing that work. Many of these interactions are important for the cell to live. Pharmaceutical companies may design drugs that can interfere with a specific interaction in order to cause an effect in the cell. Scientists are interested in measuring these interactions and we can do this by “taking a picture” of the interaction using a specialized microscope. One of the major issues with these microscopes is that it takes scientists a long time to collect pictures of these interactions. This means only a few drugs can be tested in a day. To speed up the drug discovery and testing we want to design faster microscopes that can test hundreds of drugs in a day. In my thesis I contributed to building a state-of-the-art super fast microscope. We made progress in steps, and by the third attempt we successfully measured interactions in cells in seconds! Our new microscope is ~400x faster than current technologies. We hope that this research…

Advisors/Committee Members: Fang, Qiyin, Biomedical Engineering.

Subjects/Keywords: Fluorescence Microscopy; Confocal Microsopy; Fluorescence Lifetime; Rapid Imaging; Drug Screening

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hirmiz, N. (2019). DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS. (Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/25137

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hirmiz, Nehad. “DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS.” 2019. Thesis, McMaster University. Accessed January 21, 2021. http://hdl.handle.net/11375/25137.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hirmiz, Nehad. “DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS.” 2019. Web. 21 Jan 2021.

Vancouver:

Hirmiz N. DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS. [Internet] [Thesis]. McMaster University; 2019. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/11375/25137.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hirmiz N. DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS. [Thesis]. McMaster University; 2019. Available from: http://hdl.handle.net/11375/25137

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

26. Cheng, Xiaoyu. Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications.

Degree: Chemistry, 2015, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/54301 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:34724/SOURCE02?view=true

► Quantum dots (QDs) are semiconductor nanocrystals with unique photophysical properties. Quantum dots have drawn broad research interests in the past three decades, because of their… (more)

▼ Quantum dots (QDs) are semiconductor nanocrystals with unique photophysical properties. Quantum dots have drawn broad research interests in the past three decades, because of their applications in optoelectronic devices, solar cells and fluorescent imaging agents in biomedicine. However, a major issue for the further development of this new class of materials is that many quantum dots are composed of heavy metal elements that are considered unsafe for biological purposes. Therefore, concerns over nanoparticle related toxicity have inspired the design of quantum dots made from materials with biological benign nature, such as crystalline silicon (Si).The first challenge of working with nanocrystalline silicon quantum dots (SiQDs) is the limited methods available to prepare high quality, surface functionalized nanoparticles. Among the various methods available, colloidal synthesis is of broad interests, for the simple procedures used and solution-based approaches as needed in many applications. In this thesis, chapter three and chapter four describe two new approaches of coping with this challenge, using a one-step method based on thiol-ene chemistry, and a two-step process based on copper catalyzed azide-alkyne cycloaddition (CuAAC) reaction respectively.The second challenge of applying solution synthesized SiQDs for bio-imaging is their blue photoluminescence that can be affected by biological background signals, as well as the low excitation wavelength that may induce damage to cellular structures. Most responses to this challenge have been focused on material preparation, but limited success has been achieved when solution syntheses are involved. In this thesis, chapter five presents a completely different strategy of resolving this issue by focusing on advanced microscopy. Specifically, fluorescence lifetime imaging microscopy (FLIM) is used to observe SiQDs in intracellular contexts, utilizing their long fluorescence lifetime in the context of one-photon FLIM, two-photon FLIM and energy transfer studies (FLIM-FRET).Lastly, since surface modified colloidal SiQDs is still in its infancy of development, there are still limited studies showing their applications as biosensors. In chapter six, efforts toward the preparation of the first SiQDs protease sensor is described. This is based on Förster Resonance Energy Transfer (FRET) process involving SiQDs-dye construct, where SiQDs were used as the donor, and conjugated to an organic dye acceptor via an enzyme responsive peptide linker. Advisors/Committee Members: Gooding, J. Justin, Chemistry, Faculty of Science, UNSW, Reece, Peter, Physics, Faculty of Science, UNSW, Katharina, Gaus, Faculty of Medicine, UNSW.

Subjects/Keywords: Materials; Fluorescence; Cancer; Quantum Dots; Nanoscience; Nanoparticles; Surface Modification; Imaging; Fluorescence

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cheng, X. (2015). Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/54301 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:34724/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Cheng, Xiaoyu. “Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications.” 2015. Doctoral Dissertation, University of New South Wales. Accessed January 21, 2021. http://handle.unsw.edu.au/1959.4/54301 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:34724/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Cheng, Xiaoyu. “Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications.” 2015. Web. 21 Jan 2021.

Vancouver:

Cheng X. Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications. [Internet] [Doctoral dissertation]. University of New South Wales; 2015. [cited 2021 Jan 21]. Available from: http://handle.unsw.edu.au/1959.4/54301 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:34724/SOURCE02?view=true.

Council of Science Editors:

Cheng X. Colloidal Silicon Quantum Dots: From Preparation to the Modifications of Self-Assembled Monolayers for Bio-applications. [Doctoral Dissertation]. University of New South Wales; 2015. Available from: http://handle.unsw.edu.au/1959.4/54301 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:34724/SOURCE02?view=true

University of Oklahoma

27. Li, Zheng. HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS.

Degree: PhD, 2017, University of Oklahoma

URL: http://hdl.handle.net/11244/50778

► Fluorescence in situ hybridization (FISH) is a powerful tool for visualizing and detecting genetic abnormalities. Manual scoring FISH analysis is a tedious and labor-and-time-consuming task.… (more)

Subjects/Keywords: Fluorescence Microscopy; Fluorescence in situ hybridization; Microscopic Imaging; Automated Microscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Z. (2017). HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS. (Doctoral Dissertation). University of Oklahoma. Retrieved from http://hdl.handle.net/11244/50778

Chicago Manual of Style (16^th Edition):

Li, Zheng. “HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS.” 2017. Doctoral Dissertation, University of Oklahoma. Accessed January 21, 2021. http://hdl.handle.net/11244/50778.

MLA Handbook (7^th Edition):

Li, Zheng. “HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS.” 2017. Web. 21 Jan 2021.

Vancouver:

Li Z. HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS. [Internet] [Doctoral dissertation]. University of Oklahoma; 2017. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/11244/50778.

Council of Science Editors:

Li Z. HIGH-THROUGHPUT FLUORESCENCE MICROSCOPY FOR AUTOMATED CLINICAL APPLICATIONS. [Doctoral Dissertation]. University of Oklahoma; 2017. Available from: http://hdl.handle.net/11244/50778

University of Rochester

28. Sharma, Robin. In vivo two-photon ophthalmoscopy: development and applications.

Degree: PhD, 2015, University of Rochester

URL: http://hdl.handle.net/1802/30289

► Light-sensitive molecules such as rhodopsin present in photoreceptors are responsible for detecting light and subsequently initiating multi-step biochemical cascades, namely phototransduction and the visual cycle.… (more)

▼ Light-sensitive molecules such as rhodopsin present in photoreceptors are responsible for detecting light and subsequently initiating multi-step biochemical cascades, namely phototransduction and the visual cycle. Many retinal diseases are known to be caused by a breakdown of these cascades, making them prime targets for ongoing vision restoration efforts although it has been notoriously difficult to observe their activity during light and dark in the living eye. Additionally, on its way to the photoreceptors, light has to propagate through the neurons responsible for transmitting this information to the brain. These cells are naturally translucent and although they are implicated in many diseases, current imaging techniques have been unable to image these retinal layers at a cellular scale. The neural circuitry in the retina that allows us to see is complicated, spanning across several cellular layers. Most of what we know about retinal circuitry is from electrophysiology but newer methods need to be developed to accurately measure neuronal responses in the intact, living eye with minimal visual stimulation. The goal of this work is to see the cells that allow us to see, and to develop a way to track the activity of the retina at a cellular scale in the living eye. All cells in the retina contain endogenously fluorescent molecules that are natural markers for cell health and physiology but their fluorescence cannot be accessed through conventional imaging methods because their excitation spectra lie in the ultraviolet regime outside the spectral transmission window. To target these molecules in the living eye, we have developed adaptive optics assisted two-photon fluorescence ophthalmoscopy for mouse and monkey animal models. Initially, the feasibility of tracking retinal function with this method was demonstrated with exogenous fluorophores that are sensitive to changes in intracellular calcium concentration. Next, these were deployed in the unlabeled retina to indirectly track the regeneration of rhodopsin in photoreceptors by monitoring autofluorescence from molecules involved in the visual cycle. Also, by utilizing the intrinsic contrast offered by endogenous fluorophores, two-photon imaging has also enabled visualization of various retinal structures that are otherwise invisible. With advancement of this technology, it could be used for accelerating vision restoration methods and clinical diagnostics.

Subjects/Keywords: Adaptive optics; Fluorescence; Retinal imaging; Two-photon imaging; Visual cycle

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sharma, R. (2015). In vivo two-photon ophthalmoscopy: development and applications. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/30289

Chicago Manual of Style (16^th Edition):

Sharma, Robin. “In vivo two-photon ophthalmoscopy: development and applications.” 2015. Doctoral Dissertation, University of Rochester. Accessed January 21, 2021. http://hdl.handle.net/1802/30289.

MLA Handbook (7^th Edition):

Sharma, Robin. “In vivo two-photon ophthalmoscopy: development and applications.” 2015. Web. 21 Jan 2021.

Vancouver:

Sharma R. In vivo two-photon ophthalmoscopy: development and applications. [Internet] [Doctoral dissertation]. University of Rochester; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1802/30289.

Council of Science Editors:

Sharma R. In vivo two-photon ophthalmoscopy: development and applications. [Doctoral Dissertation]. University of Rochester; 2015. Available from: http://hdl.handle.net/1802/30289

University of Toronto

29. Philp, Lauren. Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/91426

►

Treatment of endometrial cancer consists of surgery ± adjuvant therapy depending on lymph node involvement determined by lymphadenectomy. Targeting lymphadenectomy to patients with known metastases… (more)

Subjects/Keywords: Endometrial Cancer; Fluorescence Imaging; Lymphadenectomy; Metastasis; Molecular Imaging; Nanoparticle; 0574

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Philp, L. (2018). Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/91426

Chicago Manual of Style (16^th Edition):

Philp, Lauren. “Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.” 2018. Masters Thesis, University of Toronto. Accessed January 21, 2021. http://hdl.handle.net/1807/91426.

MLA Handbook (7^th Edition):

Philp, Lauren. “Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers.” 2018. Web. 21 Jan 2021.

Vancouver:

Philp L. Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1807/91426.

Council of Science Editors:

Philp L. Towards Clinical Translation of the Porphysome: Establishment of Endometrial Cancer Applications and Investigation of Metabolic Differences in Pyrolipid Isomers. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/91426

Harvard University

30. Lacy, Jessica. Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes.

Degree: Doctor of Medicine, 2014, Harvard University

URL: http://etds.lib.harvard.edu/hms/admin/view/58 ; http://nrs.harvard.edu/urn-3:HUL.InstRepos:12407616

► Poly(ADP-ribose)polymerase-1 and -2 (PARP1/2) are nuclear proteins involved in DNA repair. Tumors with defects in homologous recombination, including BRCA1- and BRCA2-deficient cancers, have been shown… (more)

Subjects/Keywords: PARP; BRCA; PET imaging; PET/CT imaging; fluorescence microscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lacy, J. (2014). Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes. (Doctoral Dissertation). Harvard University. Retrieved from http://etds.lib.harvard.edu/hms/admin/view/58 ; http://nrs.harvard.edu/urn-3:HUL.InstRepos:12407616

Chicago Manual of Style (16^th Edition):

Lacy, Jessica. “Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes.” 2014. Doctoral Dissertation, Harvard University. Accessed January 21, 2021. http://etds.lib.harvard.edu/hms/admin/view/58 ; http://nrs.harvard.edu/urn-3:HUL.InstRepos:12407616.

MLA Handbook (7^th Edition):

Lacy, Jessica. “Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes.” 2014. Web. 21 Jan 2021.

Vancouver:

Lacy J. Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes. [Internet] [Doctoral dissertation]. Harvard University; 2014. [cited 2021 Jan 21]. Available from: http://etds.lib.harvard.edu/hms/admin/view/58 ; http://nrs.harvard.edu/urn-3:HUL.InstRepos:12407616.

Council of Science Editors:

Lacy J. Imaging of PARP1/2-Overexpressing Cancers with Novel AZD2281-Derived Probes. [Doctoral Dissertation]. Harvard University; 2014. Available from: http://etds.lib.harvard.edu/hms/admin/view/58 ; http://nrs.harvard.edu/urn-3:HUL.InstRepos:12407616

Open Access Theses and Dissertations