subject:(filamentous fungi)

1. Smith, Helen A. Production of antimicrobials and antioxidants from filamentous fungi.

Degree: 2014, RIAN

URL: http://eprints.maynoothuniversity.ie/5802/

► Filamentous fungi have proven throughout history to be a vast source of potential therapeutic activities. They are recognised as nutritious, highly palatable functional foods and… (more)

▼ Filamentous fungi have proven throughout history to be a vast source of potential therapeutic activities. They are recognised as nutritious, highly palatable functional foods and are now widely accepted as an untapped source of potentially powerful natural products of pharmacological significance. In the present study, ten species of filamentous fungi were explored on the basis of their curative potential. Submerged liquid fermentation (SLF) was employed and proved to be a promising method for the production of bioactive functional compounds. Crude aqueous mycelial biomass demonstrated a broad range of antibacterial activity against pathogenically significant bacteria. Supplementation with various carbon sources at 1 % (w/v) affected the biomass production of several species significantly (p < 0.05) and in some cases also had a significant impact on cellular composition. Additionally, depending on species specificity, cell wall composition was found to affect the interaction of microbes prior to infection. Various extracts (crude, hot water and methanol) from the mycelium of each of the selected species were demonstrated as effective antioxidants. Additionally, total phenolic content positively attributed to the overall antioxidant capacity of the extracts. Bioassay-guided isolation and purification of extracts from cultured mycelia led to the identification of biologically active phenolic acids and compounds of lipid class. For effective utilisation of natural products or functional food components, quantitative information on the individual phenolic acids in each strain was generated. Compounds separated by TLC were extracted and analysed by LC/MS for fatty acid composition. The fatty acids; linoleic, palmitic, stearic and oleic acid were established as the main lipid metabolites of each fungal species. In addition, the effect of hot water and methanol extraction on fatty acid composition of the fungal cell was analysed by GC/MS. The fractionated extracts demonstrated that a combination of multiple chemical constituents yielded favourable biological activities. Submerged fermentation of mycelium from ten species of filamentous fungi proved to be an effective cultivation method for the production of bioactive and nutritional functional compounds. This research has provided information which could benefit further research in the isolation and characterisation of active chemical components of natural origin.

Subjects/Keywords: Biology; antimicrobials; antioxidants; filamentous fungi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, H. A. (2014). Production of antimicrobials and antioxidants from filamentous fungi. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/5802/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Smith, Helen A. “Production of antimicrobials and antioxidants from filamentous fungi.” 2014. Thesis, RIAN. Accessed March 08, 2021. http://eprints.maynoothuniversity.ie/5802/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Smith, Helen A. “Production of antimicrobials and antioxidants from filamentous fungi.” 2014. Web. 08 Mar 2021.

Vancouver:

Smith HA. Production of antimicrobials and antioxidants from filamentous fungi. [Internet] [Thesis]. RIAN; 2014. [cited 2021 Mar 08]. Available from: http://eprints.maynoothuniversity.ie/5802/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Smith HA. Production of antimicrobials and antioxidants from filamentous fungi. [Thesis]. RIAN; 2014. Available from: http://eprints.maynoothuniversity.ie/5802/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Smith, Helen A. Production of antimicrobials and antioxidants from filamentous fungi.

Degree: 2014, RIAN

URL: http://mural.maynoothuniversity.ie/5802/

► Filamentous fungi have proven throughout history to be a vast source of potential therapeutic activities. They are recognised as nutritious, highly palatable functional foods and… (more)

▼ Filamentous fungi have proven throughout history to be a vast source of potential therapeutic activities. They are recognised as nutritious, highly palatable functional foods and are now widely accepted as an untapped source of potentially powerful natural products of pharmacological significance. In the present study, ten species of filamentous fungi were explored on the basis of their curative potential. Submerged liquid fermentation (SLF) was employed and proved to be a promising method for the production of bioactive functional compounds. Crude aqueous mycelial biomass demonstrated a broad range of antibacterial activity against pathogenically significant bacteria. Supplementation with various carbon sources at 1 % (w/v) affected the biomass production of several species significantly (p < 0.05) and in some cases also had a significant impact on cellular composition. Additionally, depending on species specificity, cell wall composition was found to affect the interaction of microbes prior to infection. Various extracts (crude, hot water and methanol) from the mycelium of each of the selected species were demonstrated as effective antioxidants. Additionally, total phenolic content positively attributed to the overall antioxidant capacity of the extracts. Bioassay-guided isolation and purification of extracts from cultured mycelia led to the identification of biologically active phenolic acids and compounds of lipid class. For effective utilisation of natural products or functional food components, quantitative information on the individual phenolic acids in each strain was generated. Compounds separated by TLC were extracted and analysed by LC/MS for fatty acid composition. The fatty acids; linoleic, palmitic, stearic and oleic acid were established as the main lipid metabolites of each fungal species. In addition, the effect of hot water and methanol extraction on fatty acid composition of the fungal cell was analysed by GC/MS. The fractionated extracts demonstrated that a combination of multiple chemical constituents yielded favourable biological activities. Submerged fermentation of mycelium from ten species of filamentous fungi proved to be an effective cultivation method for the production of bioactive and nutritional functional compounds. This research has provided information which could benefit further research in the isolation and characterisation of active chemical components of natural origin.

Subjects/Keywords: Biology; antimicrobials; antioxidants; filamentous fungi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, H. A. (2014). Production of antimicrobials and antioxidants from filamentous fungi. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/5802/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Smith, Helen A. “Production of antimicrobials and antioxidants from filamentous fungi.” 2014. Thesis, RIAN. Accessed March 08, 2021. http://mural.maynoothuniversity.ie/5802/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Smith, Helen A. “Production of antimicrobials and antioxidants from filamentous fungi.” 2014. Web. 08 Mar 2021.

Vancouver:

Smith HA. Production of antimicrobials and antioxidants from filamentous fungi. [Internet] [Thesis]. RIAN; 2014. [cited 2021 Mar 08]. Available from: http://mural.maynoothuniversity.ie/5802/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Smith HA. Production of antimicrobials and antioxidants from filamentous fungi. [Thesis]. RIAN; 2014. Available from: http://mural.maynoothuniversity.ie/5802/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Carolina – Greensboro

3. Bukhari, Rabia M. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.

Degree: 2013, University of North Carolina – Greensboro

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=9977

► Natural products have played a significant role in the drug discovery especially in the area of cancer. Over the period of 1940-2010, 50% of the… (more)

Subjects/Keywords: Environmental chemistry; Pharmaceutical chemistry; Filamentous fungi – Biotechnology; Filamentous fungi – Cytology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bukhari, R. M. (2013). Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. (Masters Thesis). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=9977

Chicago Manual of Style (16^th Edition):

Bukhari, Rabia M. “Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.” 2013. Masters Thesis, University of North Carolina – Greensboro. Accessed March 08, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=9977.

MLA Handbook (7^th Edition):

Bukhari, Rabia M. “Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.” 2013. Web. 08 Mar 2021.

Vancouver:

Bukhari RM. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. [Internet] [Masters thesis]. University of North Carolina – Greensboro; 2013. [cited 2021 Mar 08]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=9977.

Council of Science Editors:

Bukhari RM. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. [Masters Thesis]. University of North Carolina – Greensboro; 2013. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=9977

University of Adelaide

4. Denton, Jai Andrew. From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement.

Degree: 2011, University of Adelaide

URL: http://hdl.handle.net/2440/67239

► Carbon catabolite repression is a regulatory system whereby an organism can sequentially utilise carbon sources based on their available energy. This system results in the… (more)

▼ Carbon catabolite repression is a regulatory system whereby an organism can sequentially utilise carbon sources based on their available energy. This system results in the repression of genes encoding enzymes responsible for the utilisation of poorer carbon sources when preferable ones are available. Carbon catabolite repression has been extensively studied in the filamentous fungus Aspergillus nidulans. Repression is mediated via CreA, a zinc finger DNA binding protein, which is in turn, either directly or indirectly, regulated by an ubiquitination / deubiquitination system involving CreB, CreC and CreD. Previous work demonstrated that the A. nidulans genome contains a CreD homologue, ApyA, and that both of these proteins interact with an ubiquitin ligase, HulA. This relationship was proposed to be similar to Rod1p and Rog3p and their interaction with the ubiquitin ligase Rsp5p in Saccharomyces cerevisiae. Both apyA and hulA were targeted for disruption to facilitate phenotypic analysis and the study of epistatic interactions. Deletion of hulA was shown to be lethal in an A. nidulans haploid, but viable as a heterozygote in an A. nidulans diploid. The only detectable phenotypes of this deletion in a heterozygous diploid were increased sensitivity to molybdate and acriflavine. A strain containing a disruption of apyA did not demonstrate any detectable phenotypes, however, the apyA disruption allele showed epistatic interactions with mutations in creB, creC and creD. The disruption of apyA partially suppressed the phenotype of sensitivity to allyl alcohol in the presence of glucose displayed by strains containing mutations in creB and creC. However, the level of suppression exhibited by the disruption of apyA was not as strong as that shown by the creD34 mutation. A strain containing mutations in both creD and apyA demonstrated severe morphological deficiencies on minimal media as well as stronger resistance to acriflavine than creD34 alone, and resistance to molybdate. Bioinformatic analysis of CreD and CreD‐like proteins, including ApyA, from sequenced members of the Aspergilli and Rod1p, Rog3p and related proteins from members of Saccharomycetes suggested that the arrestin-like proteins, a group to which these belong, are subject to frequent gene duplication events. The number and range of sequenced fungal genomes also allowed a bioinformatic examination of the conservation of proteins involved in the carbon repression mechanisms across the fungal kingdom. A homologue of CreA was identified only within the members of Ascomycota that were examined, but putative homologues of CreB and CreC were identified across the fungal kingdom. The Saccharomycetes were an exception to this as a CreC homologue was not indentified and the CreB homologue was highly divergent or absent. The filamentous fungus, Trichoderma reesei is an important source of cellulases for use in the textile and alternative fuel industries. Previous studies have suggested a benefit for the manipulation of carbon catabolite repression for strain… Advisors/Committee Members: Kelly, Joan Maree (advisor), School of Molecular and Biomedical Science (school).

Subjects/Keywords: genetics; filamentous fungi; gene regulation; biotechnology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Denton, J. A. (2011). From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/67239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Denton, Jai Andrew. “From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement.” 2011. Thesis, University of Adelaide. Accessed March 08, 2021. http://hdl.handle.net/2440/67239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Denton, Jai Andrew. “From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement.” 2011. Web. 08 Mar 2021.

Vancouver:

Denton JA. From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement. [Internet] [Thesis]. University of Adelaide; 2011. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/2440/67239.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Denton JA. From model organism to industrial workhorse: analysis of genes in Aspergillus nidulans and disruption of cre2 for Trichoderma reesei strain improvement. [Thesis]. University of Adelaide; 2011. Available from: http://hdl.handle.net/2440/67239

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Bukhari, Rabia M. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.

Degree: 2013, NC Docks

URL: http://libres.uncg.edu/ir/uncg/f/Bukhari_uncg_0154M_11205.pdf

► Natural products have played a significant role in the drug discovery especially in the area of cancer. Over the period of 1940-2010, 50% of the… (more)

Subjects/Keywords: Environmental chemistry; Pharmaceutical chemistry; Filamentous fungi $x Biotechnology; Filamentous fungi $x Cytology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bukhari, R. M. (2013). Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Bukhari_uncg_0154M_11205.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bukhari, Rabia M. “Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.” 2013. Thesis, NC Docks. Accessed March 08, 2021. http://libres.uncg.edu/ir/uncg/f/Bukhari_uncg_0154M_11205.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bukhari, Rabia M. “Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry.” 2013. Web. 08 Mar 2021.

Vancouver:

Bukhari RM. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. [Internet] [Thesis]. NC Docks; 2013. [cited 2021 Mar 08]. Available from: http://libres.uncg.edu/ir/uncg/f/Bukhari_uncg_0154M_11205.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bukhari RM. Bioactive natural products: isolation of fungal secondary metabolites and applications of green chemistry. [Thesis]. NC Docks; 2013. Available from: http://libres.uncg.edu/ir/uncg/f/Bukhari_uncg_0154M_11205.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Stellenbosch University

6. Swiegers, Jan Hendrik. Carnitine in yeast and filamentous fungi.

Degree: Microbiology, 2003, Stellenbosch University

URL: http://hdl.handle.net/10019.1/49753

►

Dissertation (PhD) – University of Stellenbosch, 2003.

ENGLISH ABSTRACT: In the yeast Saccharomyces cerevtstee, two biochemical pathways ensure that activated cytoplasmic or peroxisomal acetyl-groups are made… (more)

▼

Dissertation (PhD) – University of Stellenbosch, 2003.

ENGLISH ABSTRACT: In the yeast Saccharomyces cerevtstee, two biochemical pathways ensure that activated cytoplasmic or peroxisomal acetyl-groups are made available for mitochondrial energy production when the cells utilise non-fermentable carbon sources. The first pathway is the glyoxylate cycle, where two activated acetyl-groups are incorporated into each cycle, which releases a C4 intermediate. This intermediate is then transported to the mitochondria where it can enter the tricarboxylic acid cycle. The second pathway is the carnitine shuttle. Activated acetyl-groups react with carnitine to form acetylcarnitine, which is then transported to the mitochondria where the acetyl group is transferred. In this study it was shown that the deletion of the glyoxylate cycle specific citrate synthase, encoded by CIT2, results in a strain that is dependent on carnitine for growth on non-fermentable carbon sources. Using a /::"cit2 strain, mutants affected in carnitine-dependent metabolic activities were generated. Complementation of the mutants with a genomic library resulted in the identification of four genes involved in the carnitine shuttle. These include: (i) the mitochondrial and peroxisomal carnitine acetyltransferase, encoded by CAT2; (ii) the outer-mitochondrial carnitine acetyltransferase, encoded by YA T1; (iii) the mitochondrial carnitine translocase, encoded by CRC1; and (iv) a newly identified carnitine acetyltransferase, encoded by YAT2. All three carnitine acetyltransferases are essential in a carnitine-dependent strain. The dependence on exogenous carnitine of the /::"cit2 strain when grown on nonfermentable carbon sources suggested that S. cerevisiae does not biosynthesise carnitine. Measurements using electrospray mass spectrometry confirmed this hypothesis. As a result an investigation was initiated into carnitine biosynthesis in order to genetically engineer a S. cerevisiae strain that could endogenously biosynthesise carnitine. The filamentous fungus, Neurospora crassa, was one of the first organisms used in the seventies to identify the precursor and intermediates of carnitine biosynthesis. However, it was only about twenty years later that the first genes encoding these enzymes where characterised. Carnitine biosynthesis is a four-step process, which starts with trimethyllysine as precursor. Trimethyllysine is converted to hydroxytrimethyllysine by the enzyme trimethyllysine hydroxylase (TMLH). Hydroxytrimethyllysine is cleaved to trimethylamino-butyraldehyde by the hydroxytrimethyllysine aldolase (HTMLA) releasing glycine. Trimethylaminobutyraldehyde is dehydrogenated to trimethylamino-butyrate (y-butyrobetaine) by trimethylamino-butyraldehyde dehydrogenase (TMABA-DH). In the last step, ybutyrobetaine is converted to t-carnltine by y-butyrobetaine hydroxylase (BBH). The N. crassa TMLH homologue was identified in the genome database based on the protein sequence homology of the human TMLH. Due to the high…

Advisors/Committee Members: Bauer, F. F., Pretorius, I. S., Stellenbosch University. Faculty of Science. Dept. of Microbiology..

Subjects/Keywords: Carnitine – Synthesis; Yeast fungi; Filamentous fungi

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APA (6^th Edition):

Swiegers, J. H. (2003). Carnitine in yeast and filamentous fungi. (Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/49753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Swiegers, Jan Hendrik. “Carnitine in yeast and filamentous fungi.” 2003. Thesis, Stellenbosch University. Accessed March 08, 2021. http://hdl.handle.net/10019.1/49753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Swiegers, Jan Hendrik. “Carnitine in yeast and filamentous fungi.” 2003. Web. 08 Mar 2021.

Vancouver:

Swiegers JH. Carnitine in yeast and filamentous fungi. [Internet] [Thesis]. Stellenbosch University; 2003. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10019.1/49753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Swiegers JH. Carnitine in yeast and filamentous fungi. [Thesis]. Stellenbosch University; 2003. Available from: http://hdl.handle.net/10019.1/49753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Ravula, Vamsi Krishna. Study on fungal pellet morphology and its industrial applications.

Degree: Engineering and Business, 2017, University of Borås

URL: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13497

► Mycelial pellet formation by filamentous fungi is one of the most researched topics in fungal biotechnology research. Pellets are generally formed as a result… (more)

Subjects/Keywords: Filamentous fungi; Ascomycetes; Edible fungi; Pellet; Neurospora intermedia; Other Engineering and Technologies; Annan teknik

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ravula, V. K. (2017). Study on fungal pellet morphology and its industrial applications. (Thesis). University of Borås. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ravula, Vamsi Krishna. “Study on fungal pellet morphology and its industrial applications.” 2017. Thesis, University of Borås. Accessed March 08, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ravula, Vamsi Krishna. “Study on fungal pellet morphology and its industrial applications.” 2017. Web. 08 Mar 2021.

Vancouver:

Ravula VK. Study on fungal pellet morphology and its industrial applications. [Internet] [Thesis]. University of Borås; 2017. [cited 2021 Mar 08]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13497.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ravula VK. Study on fungal pellet morphology and its industrial applications. [Thesis]. University of Borås; 2017. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-13497

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

8. Skinner, Kristin M. Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species.

Degree: PhD, Botany and Plant Pathology, 2007, Oregon State University

URL: http://hdl.handle.net/1957/4952

► Graphium sp., a eukaryotic alkanotroph, is able to oxidize small-molecular weight gaseous n-alkanes, diethyl ether and the branched ether, methyl tert butyl ether (MTBE). However,… (more)

▼ Graphium sp., a eukaryotic alkanotroph, is able to oxidize small-molecular weight gaseous n-alkanes, diethyl ether and the branched ether, methyl tert butyl ether (MTBE). However, information regarding the biochemistry of fungal-mediated alkane and ether metabolism is limited, and questions regarding the identity of alkane oxidation catalysts and the genetic underpinnings of alkane metabolism are unresolved. The objectives of this investigation were to refine the pathway and the regulation of MTBE metabolism, to further define the substrate range and to identify and characterize the hydroxylase responsible for alkane and ether oxidation in this Graphium species. We found that Graphium oxidizes MTBE through a novel variation of an existing pathway first described in n-alkane-grown Mycobacterium vaccae JOB5. However, the fungus is unable to utilize the products of MTBE metabolism, resulting in the accumulation of potentially toxic intermediates. We also found that the regulatory effects of MTBE oxidation intermediates proposed for other MTBE- degrading organisms do not impact Graphium-mediated MTBE metabolism and thus are not universally relevant mechanisms for MTBE-degrading organisms. Given that Graphium is able to degrade MTBE and diethyl ether, we investigated the ability of the fungus to degrade environmentally relevant cyclic ethers including tetrahydrofuran (THF) and 1,4-dioxane (14D). Our investigation of cyclic ether metabolism revealed that Graphium sp. utilizes THF as a sole source of carbon and energy under aerobic conditions via the THF metabolic pathway used by Rhodococcus ruber and two Pseudonocardia strains. Although Graphium sp. was unable to grow on 14D, it was able to cometabolize this compound after growth on either THF or alkanes. The results of our investigations regarding cyclic ether and MTBE metabolism suggested that the metabolic pathways that process these compounds are superimposed on the alkane oxidation pathway. Because monooxygenase-catalyzed substrate activation is both the first and the rate- determining step of these pathways, an additional aim of this investigation was to identify, clone and characterize the gene encoding the alkane monooxygenase from this Graphium sp. Prokaryotic alkanotrophs oxidize alkanes mainly through diiron and copper-containing monooxygenases. Unlike prokaryotes, in Graphium sp., we found that the initial oxidation of alkanes is catalyzed by a cytochrome P450 alkane monooxygenase. This is the first report of a cytochrome P450 monooxygenase that is able to oxidize gaseous n-alkanes. To further characterize this novel enzyme, we also estimated the regiospecificity of alkane oxidation and determined that although the majority of hydroxylation events result in terminal carbon oxidation, a significant portion of these events result in subterminal oxidation. Subterminal oxidation can produce metabolites that are unpalatable and possibly toxic. Taken as a whole, the results of these investigations significantly extend the… Advisors/Committee Members: Ciuffetti, Lynda (advisor), Arp, Daniel (committee member).

Subjects/Keywords: Graphium sp.; Filamentous fungi – Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Skinner, K. M. (2007). Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/4952

Chicago Manual of Style (16^th Edition):

Skinner, Kristin M. “Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species.” 2007. Doctoral Dissertation, Oregon State University. Accessed March 08, 2021. http://hdl.handle.net/1957/4952.

MLA Handbook (7^th Edition):

Skinner, Kristin M. “Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species.” 2007. Web. 08 Mar 2021.

Vancouver:

Skinner KM. Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species. [Internet] [Doctoral dissertation]. Oregon State University; 2007. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1957/4952.

Council of Science Editors:

Skinner KM. Characterization of the molecular foundations and biochemistry of alkane and ether oxidation in a filamentous fungus, a Graphium species. [Doctoral Dissertation]. Oregon State University; 2007. Available from: http://hdl.handle.net/1957/4952

Rhodes University

9. Flanagan, Shane Patrick. Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A.

Degree: MS, Faculty of Science, Biochemistry, Microbiology and Biotechnology, 2010, Rhodes University

URL: http://hdl.handle.net/10962/d1013328

► Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine.… (more)

▼ Ochratoxin A is the most potent chlorinated derivative of the ochratoxin group, consisting of a 5'-chlorinated dihydroisocoumarin moiety linked by an amide bond to l-phenylalanine. Produced as a secondary fungal metabolite by several species of Aspergillus and Penicillium, ochratoxin A has been shown to readily contaminate a large variety of commodities including cereals, groundnuts, dried fruit, spices and coffee. This has led to widespread contamination of ochratoxin in wine, beer, milk and meat products. As ochratoxin A is a potent nephrotoxin exhibiting teratogenic and carcinogenic properties, the development of a rapid screening platform for the cost effective control of ochratoxin A content in foodstuffs is therefore required. The evaluation of metallophthalocyanine and carbon nanotube electrode modification toward the development of a nanostructured biosensor capable of enhancing the electrochemical detection of ochratoxin A in complex media is presented. Cyclic voltammetry at a glassy carbon electrode allowed for the optimization of detection parameters including pH and type of supporting electrolyte. Britton-Robinson buffer was found to be the most suitable supporting electrolyte in terms of sensitivity and reproducibility obtaining a LOD of 0.28 μM as determined by differential pulse voltammetry. Subsequent analysis determined the dependence of OTA oxidation on pH in acidic media which proceeds with the transfer of two electrons to form a quinone/hydroquinone couple shown to adsorb to the electrode surface. Passivation of the electrode through adsorption of oxidation products was shown to severely limit the detection of OTA upon successive detection cycles. Comparison of various metallophthalocyanine modifiers showed an increase in sensitivity toward the detection of OTA at phthalocyanine complexes with metal based redox processes. However with the exception of NiPc and CoTCPc complexes, phthalocyanine modification was limited by the increase in deviation of current response and extent of fouling. NiPc modification showed an increase in sensitivity by two fold with fouling characteristics comparable to an unmodified electrode while low improvements in fouling was observed at CoTCPc modified electrodes with sensitivity in detection comparable to an unmodified electrode.Modification of the electrode with multi- and single walled carbon nanotubes produced a significant increase in sensitivity toward the detection of ochratoxin A. The electrocatalytic activity of nanotube modifiers was attributed to the increase in surface area and to the addition of oxygenated functional groups upon acid treatment as confirmed by Raman spectroscopy. Acid functionalization of the carbon nanotubes for a period of two hours produced the greatest increase in sensitivity obtaining a respective LOD of 0.09 μM and 0.03 μM for analysis of ochratoxin A at multi- and single walled carbon nanotube modified electrodes. Centrifugal purification of carbon nanotubes was deemed necessary to improve the electrocatalytic activity of the… Advisors/Committee Members: Limson, Janice.

Subjects/Keywords: Ochratoxins; Filamentous fungi; Electrochemical sensors; Nanostructured materials; Catalysts; Food contamination

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APA (6^th Edition):

Flanagan, S. P. (2010). Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A. (Masters Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1013328

Chicago Manual of Style (16^th Edition):

Flanagan, Shane Patrick. “Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A.” 2010. Masters Thesis, Rhodes University. Accessed March 08, 2021. http://hdl.handle.net/10962/d1013328.

MLA Handbook (7^th Edition):

Flanagan, Shane Patrick. “Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A.” 2010. Web. 08 Mar 2021.

Vancouver:

Flanagan SP. Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A. [Internet] [Masters thesis]. Rhodes University; 2010. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10962/d1013328.

Council of Science Editors:

Flanagan SP. Application of catalysts and nanomaterials in the design of an electrochemical sensor for ochratoxin A. [Masters Thesis]. Rhodes University; 2010. Available from: http://hdl.handle.net/10962/d1013328

Universidade Federal do Maranhão

10. Diogo Marcelo Lima Ribeiro. CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA).

Degree: 2011, Universidade Federal do Maranhão

URL: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=626

►

A Tiquira é uma bebida alcoólica destilada e preparada a partir da sacarificação e posterior fermentação da mandioca. Os processos de sacarificação e fermentação são… (more)

▼

A Tiquira é uma bebida alcoólica destilada e preparada a partir da sacarificação e posterior fermentação da mandioca. Os processos de sacarificação e fermentação são realizados por micro-organismos que se desenvolvem naturalmente nos beijus expostos ao meio ambiente. O crescimento desses micro-organismos ocorre por um período aproximado de 8 dias e, como são diversas as linhagens coletadas, o rendimento do processo bem como a qualidade do destilado ficam comprometidos. O objetivo deste trabalho é, portanto, o de contribuir para a obtenção de uma maior qualidade da aguardente de mandioca (Tiquira) produzida no estado do Maranhão através da identificação e seleção dos melhores micro-organismos sacarificantes e fermentativos empregados no processo artesanal. As cepas selecionadas foram submetidas a estudos, onde identificamos a presença de fungos filamentosos, bactérias e uma levedura. Foram identificadas 3 (três) espécies de fungos filamentosos: Aspergillus niger, Aspergillus flavus e Rhizopus oryzae, os quais foram testados perante o seu poder de esporulação em diferentes meios de cultura a 30 °C, tendo o meio SDA (Saboroud Dextrose Ágar) fornecido a melhor composição para o obtenção do crescimento dos fungos mais rapidamente. As amostras de R. oryzae, A. niger, A. flavus e a mistura destes esporos, que previamente mostraram ser melhores produtoras enzimáticas foram selecionadas para testes de sacarificação do amido para avaliar e confirmar a capacidade de conversão à glicose. A cepa R. oryzae alcançou um valor de conversão de 78,02% em média utilizando-se uma temperatura padrão de 30 °C, quantidade de esporos 5,5 x 107, pH 5 e 50g/l de amido solúvel, obtendo o maior rendimento de sacarificação, seguida pelo A. flavus 71,55% , A. niger 57,17% e mistura dos esporos 48,02%. Portanto, evidencia-se que a utilização de micro-organismos filamentosos autóctones torna-se uma opção para uso em sacarificação de amostras amiláceas.

The Tiquira is a distilled alcoholic beverage prepared from the saccharification and subsequent fermentation of cassava. The processes of saccharification and fermentation are performed by micro-organisms that grow naturally in beijus exposed to the environment. The growth of these micro-organisms occurs over a period of approximately 8 days and as many strains are collected, the process yield and the quality of distillate are compromised. The objective is therefore to improve the quality of spirit cassava (Tiquira) produced in the state of Maranhão through the identification and selection of the best micro-organisms and fermentation and saccarifiying employed in artisanal process. The selected strains were subjected to studies, which identified the presence of filamentous fungi, bacteria and yeast. We identified three (3) species of filamentous fungi: Aspergillus niger, Aspergillus flavus and Rhizopus oryzae, which were tested before their power of sporulation in different culture media at 30 °C, and the SDA medium provided the best composition for obtaining the fungal growth more quickly. Samples of…

Advisors/Committee Members: CICERO WELLINGTON BRITO BEZERRA.

Subjects/Keywords: Amido; Sacarificação; Fungos Filamentosos; Tiquira; QUIMICA; Starch; Saccharification; filamentous fungi; tiquira

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ribeiro, D. M. L. (2011). CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA). (Thesis). Universidade Federal do Maranhão. Retrieved from http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ribeiro, Diogo Marcelo Lima. “CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA).” 2011. Thesis, Universidade Federal do Maranhão. Accessed March 08, 2021. http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ribeiro, Diogo Marcelo Lima. “CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA).” 2011. Web. 08 Mar 2021.

Vancouver:

Ribeiro DML. CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA). [Internet] [Thesis]. Universidade Federal do Maranhão; 2011. [cited 2021 Mar 08]. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=626.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ribeiro DML. CARACTERIZAÇÃO E COMPORTAMENTO SACARIFICANTE DA FLORA MICROBIANA EMPREGADA NA FABRICAÇÃO DA AGUARDENTE DE MANDIOCA (TIQUIRA). [Thesis]. Universidade Federal do Maranhão; 2011. Available from: http://www.tedebc.ufma.br//tde_busca/arquivo.php?codArquivo=626

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

11. Mackay, Stephen. Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi .

Degree: 2015, University of the Western Cape

URL: http://hdl.handle.net/11394/5314

► Biofuel production from microalgae is currently not economically competitive with fossil fuels due to high operational costs. A sustainable system needs to be developed which… (more)

Subjects/Keywords: Chlorella sorokiniana; Isaria fumorosea; Microalgae; Filamentous fungi; Lichen

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APA (6^th Edition):

Mackay, S. (2015). Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/5314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mackay, Stephen. “Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi .” 2015. Thesis, University of the Western Cape. Accessed March 08, 2021. http://hdl.handle.net/11394/5314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mackay, Stephen. “Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi .” 2015. Web. 08 Mar 2021.

Vancouver:

Mackay S. Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi . [Internet] [Thesis]. University of the Western Cape; 2015. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/11394/5314.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mackay S. Assisted flocculation of Chlorella Sorokiniana by co-culture with filamentous fungi . [Thesis]. University of the Western Cape; 2015. Available from: http://hdl.handle.net/11394/5314

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Eades, Caleb Joshua. Characterization of the alpha-mannosidase gene family in filamentous fungi.

Degree: Department of Biology, 2018, University of Victoria

URL: https://dspace.library.uvic.ca//handle/1828/9431

► Protein N-glycosylation, which is ubiquitous in eukaryotes, is a complex pathway involving numerous gene families. Early stages of the glycosylation pathway show a high degree… (more)

▼ Protein N-glycosylation, which is ubiquitous in eukaryotes, is a complex pathway involving numerous gene families. Early stages of the glycosylation pathway show a high degree of conservation among eukaryotes, yet diversification of the number and size of gene families involved in the later stages of the pathway has led to the evolution of increasingly complex N-glycan structures and functions in various organisms. The overall purpose of this research project has been to characterize the diversity within the α-mannosidase gene family of filamentous fungi. The α-1,2-mannosidases are involved in mannose removal in the intermediate stages of the N-glycosylation pathways, and diversification of this gene family may have provided the first significant divergence in these pathways among major lineages. Four novel α-mannosidases were identified and characterized from the filamentous fungus Aspergillus nidulans. These genes were designated Class II α-mannosidase, Class I α-1,2-mannosidase IA, Class I α-1,2-mannosidase IB and Class I α-1,2-mannosidase IC, based on their similarity to other Class I and Class II α-mannosidase sequences. The Class II α-mannosidase was highly similar to the rat ER/cytosolic and yeast vacuolar Class II α-mannosidases, and these three proteins formed a phylogenetically distinct subgroup, Class IIC. The Class I enzymes were highly related to each other, and to other fungal Class I α-1,2-mannosidases. Phylogenetic analysis indicates these genes duplicated and diverged subsequent to the divergence of fungi from insects and mammals. In addition to this research on A. nidulans, a single Class I α-1,2-mannosidase was identified and characterized from the Dutch Elm pathogen, Ophiostoma novo-ulmi, which was highly related to the A. nidulans Class I α-1,2-mannosidase IA and IC enzymes, and less so to the A. nidulans Class I α-1,2-mannosidase IB. Analysis of the function and/or biochemical properties of these enzymes was examined using several methods. Disruption and overexpression of the A. nidulans Class IIC α-mannosidase did not have any noticeable effect on the growth or morphology of the organism, indicating that this gene was not essential for growth. Biochemical characterization of the A. nidulans Class I α-1,2-mannosidase IC was initiated by recombinant secretion of the enzyme into culture media. Successful expression of the enzyme showed that the α-1,2-mannosidase IC did not exert any cytotoxic effects when overexpressed, suggesting that high levels of expression and purification should be feasible. Finally, disruption of the Class I α-1,2-mannosidase from O. novo-ulmi slightly altered the morphology of the organism, but was not lethal. The possible presence of multiple Class I α-1,2-mannosidases in this organism could explain the non-lethality of this mutation. Elucidation of the N-glycosylation pathways of A. nidulans may be useful in host strain improvement for heterologous protein expression systems. Modulation of the N-glycosylation pathways to produce specific N-glycan structures… Advisors/Committee Members: Hintz, William E. A. (supervisor).

Subjects/Keywords: Mannosidases; Filamentous fungi

…2000. Characterization of the a-mannosidase gene family in filamentous fungi: N-glycan… …poorly characterized in filamentous fungi. Research in filamentous fungi includes… …pathway. Further characterization of the N-glycosylation pathway in filamentous fungi is… …numbers of mannose residues (Herscovics and Orlean, 1993), whereas filamentous fungi… …characterized in filamentous fungi. Protein N-glycosylation occurs when an oligosaccharide precursor…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Eades, C. J. (2018). Characterization of the alpha-mannosidase gene family in filamentous fungi. (Thesis). University of Victoria. Retrieved from https://dspace.library.uvic.ca//handle/1828/9431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Eades, Caleb Joshua. “Characterization of the alpha-mannosidase gene family in filamentous fungi.” 2018. Thesis, University of Victoria. Accessed March 08, 2021. https://dspace.library.uvic.ca//handle/1828/9431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Eades, Caleb Joshua. “Characterization of the alpha-mannosidase gene family in filamentous fungi.” 2018. Web. 08 Mar 2021.

Vancouver:

Eades CJ. Characterization of the alpha-mannosidase gene family in filamentous fungi. [Internet] [Thesis]. University of Victoria; 2018. [cited 2021 Mar 08]. Available from: https://dspace.library.uvic.ca//handle/1828/9431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Eades CJ. Characterization of the alpha-mannosidase gene family in filamentous fungi. [Thesis]. University of Victoria; 2018. Available from: https://dspace.library.uvic.ca//handle/1828/9431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

13. Wang, Dongwei. Kompromisse bei fadenförmigen Bodenpilzen.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27326

► Diese Doktorarbeit befasst sich mit der Fragestellung, wie sich die Merkmalsbeschaffenheit von filamentösen Pilzen auf ihre Ökologie und spezifischen Lebensgeschichtenstrategien („life history strategy“) auswirkt. Zu… (more)

▼ Diese Doktorarbeit befasst sich mit der Fragestellung, wie sich die Merkmalsbeschaffenheit von filamentösen Pilzen auf ihre Ökologie und spezifischen Lebensgeschichtenstrategien („life history strategy“) auswirkt. Zu diesem Zweck wurden 30 Stämme filamentöser Pilze untersuchte, die von der AG Rillig aus Grasslandboden (Mallnow, Deutschland) isoliert wurden. Diese Pilzstämme wurden auf ihre Reaktion hinsichtlich verschiedener Umweltstimuli untersucht. Bei diesen handelte es sich um Erwärmung und mechanische Störung bzw. Beschädigung des Pilzmyzels. In Kapitel 1 wird auf Basis einer Literatursynthese zum Thema Pilzmerkmale, das grundlegende Fundament dieses Forschungsvorhabens und der zugrundeliegenden Methodik herausgearbeitet. In Kapitel 2 wird die Fähigkeit der Ressourcenzuweisung der 33 Pilzstämme erforscht. Dabei konnten wir zeigen, dass schnell wachsende Pilzstämme eine Tendenz zu lokalem Wachstum aufweisen, während langsam wachsende Stämme bevorzugt in ihre Umgebung auf der Suche nach weiteren Ressourcen expandieren. In Kapitel 3, wurden die Auswirkungen von wiederholter, mechanischer Myzelbeschädigung auf die Fitness von schnell und langsam wachsenden Pilzstämmen untersucht. Dabei zeigt sich, dass schnell wachsende Stämme eine geringe Effizienz in der Kohlenstoffnutzung und eine höhere Sensitivität gegenüber der zugefügten mechanischen Beschädigung aufweisen als ihre langsamer wachsenden Gegenstücke. In Kapitel 4 wird die Temperatursensitivität der 30 Pilzstämme mittels Q10-Indikator erhoben. Es zeigt sich, dass die Temperatursensitivität negativ mit der radialen Koloniewachstumsrate und positiv mit der optimalen Wachstumtemperatur korreliert. In Kapitel 5 wird eine neue Methodik zur Darstellung der Lebensgeschichtenmerkmale von Pilzen mittels sogenanntem CSR-Model vorgestellt; das CRS-Model umfasst dabei Merkmal, die die kompetetive, Stresstolerenz- und Ruderalkapazität abbilden. Es zeigt sich, dass das zweidimensionale CSR-Model das Potenzial hat, mehr Informationen zu enthalten, als das eindimensionale Gegenmodel der r-K-Strategien. Jedoch sind die CSR-Merkmale nicht phylogenetisch konserviert. In Kapitel 6 werden die Ergebnisse dieser Arbeit in den breiten Literaturkontext gestellt und die Schlussfolgerungen der vorangegangenen Kapitel zusammengefasst. Advisors/Committee Members: female (gender), Rillig, Matthias (firstReferee), Forstreuter, Manfred (furtherReferee).

Subjects/Keywords: Trade-offs; filamentous fungi; competitive-stress tolerant-ruderal; ddc:500

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APA (6^th Edition):

Wang, D. (2020). Kompromisse bei fadenförmigen Bodenpilzen. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Dongwei. “Kompromisse bei fadenförmigen Bodenpilzen.” 2020. Thesis, Freie Universität Berlin. Accessed March 08, 2021. http://dx.doi.org/10.17169/refubium-27326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Dongwei. “Kompromisse bei fadenförmigen Bodenpilzen.” 2020. Web. 08 Mar 2021.

Vancouver:

Wang D. Kompromisse bei fadenförmigen Bodenpilzen. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Mar 08]. Available from: http://dx.doi.org/10.17169/refubium-27326.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang D. Kompromisse bei fadenförmigen Bodenpilzen. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27326

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

14. Cao, Yu. Towards a Fundamental Understanding of Solid State Bioprocessing.

Degree: 2019, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:318309

► Solid state fermentation and solid state bioprocessing have been recognised for their unique features. However, there are several unsolved challenges which have prevented their widespread… (more)

▼ Solid state fermentation and solid state bioprocessing have been recognised for their unique features. However, there are several unsolved challenges which have prevented their widespread adoptions. In order to address these challenges, an investigation has been conducted, aiming at both identifying and addressing the problems. Two common applications of solid state bioprocessing, namely reducing toxins in raw materials and producing generic microbial feedstocks, were studied and are reported in Part A of the thesis (Chapter 3 and Chapter 4). The findings reported in Chapter 3 show that by applying a two stage solid state bioprocess, the major toxin in rapeseed meal can be reduced to a level suitable for animal consumption. The first stage, pre-incubation, greatly assisted toxin reduction in the second stage (fermentation), through the addition of water into the substrate. Although the exact mechanisms were not elucidated, it was clear that water is a critical factor in the process. In Chapter 4, a three stage solid state bioprocess was used to produce a generic microbial feedstock from mixed rapeseed meal and sugarcane bagasse. The main findings from this study were that such processes are highly variable, and that the performance of solid state fermentation is highly influenced by the microscopic environment of the microorganism. This led to the conclusion that a more fundamental understanding of the system is required. To obtain a better fundamental understanding, the effect of water on fungi cultivated on solid substrates (water bioavailability), was investigated in Part B of the thesis which includes theoretical and experimental investigations. The following theory has been developed from the findings. â€œFungal growth can only consume water from the surrounding local micro-environment. As germination and growth occur, they result in a modest depletion of water from the immediate vicinity. This creates a driving force for water to migrate from the bulk substrate (usually through diffusion), or to be absorbed from the gas phase, to replenish the water that has been consumed. The driving force within the substrate increases with fungal water consumption, while water absorption from the gas phase is driven both by the gas phase relative humidity and presence/absence of water at the substrate surface. Meanwhile, the resistance to mass transfer is greatly affected by both chemical and physical properties of the substrate. Fungal growth can continue, if, and only if water within their immediate vicinity can be replenished.â€ Experiments showed that water bioavailability cannot be fully represented by terms such as â€œwater contentâ€ and â€œwater activityâ€, but is affected by the presence of water in the immediate vicinity of the fungus. This is under the influence of substrate chemical properties and consequently affects germination and early growth. It is also affected by mobility of water through the substrate which impacts on growth rate in the absence of gas phase water. Further, if there is water in the gas… Advisors/Committee Members: SAIANI, ALINE AF, Webb, Colin, Saiani, Aline.

Subjects/Keywords: Solid state fermentation; Water; Dynamic vapour sorption; Microbial activity; filamentous fungi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cao, Y. (2019). Towards a Fundamental Understanding of Solid State Bioprocessing. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:318309

Chicago Manual of Style (16^th Edition):

Cao, Yu. “Towards a Fundamental Understanding of Solid State Bioprocessing.” 2019. Doctoral Dissertation, University of Manchester. Accessed March 08, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:318309.

MLA Handbook (7^th Edition):

Cao, Yu. “Towards a Fundamental Understanding of Solid State Bioprocessing.” 2019. Web. 08 Mar 2021.

Vancouver:

Cao Y. Towards a Fundamental Understanding of Solid State Bioprocessing. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Mar 08]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:318309.

Council of Science Editors:

Cao Y. Towards a Fundamental Understanding of Solid State Bioprocessing. [Doctoral Dissertation]. University of Manchester; 2019. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:318309

Swedish University of Agricultural Sciences

15. Schenck, Jessica. Filamentous fungi in wrapped forages.

Degree: 2019, Swedish University of Agricultural Sciences

URL: https://pub.epsilon.slu.se/16413/

► Wrapped forages of higher dry matter (DM) concentrations (> 50 %), also referred to as haylage are common in Sweden and Norway. Such forages are… (more)

▼ Wrapped forages of higher dry matter (DM) concentrations (> 50 %), also referred to as haylage are common in Sweden and Norway. Such forages are preserved by a combination of semi-drying and anaerobic storage leading to an environment that may affect the composition of filamentous fungi differently than in hay or silage. The aim of this research was to identify fungal species in wrapped forages with higher DM concentration in relation to forage production and management factors. In the first study, the effect of plant maturity at harvest on microbial composition of forage was investigated. The microbial composition of fresh herbage and conserved haylage was compared for three different harvest times (June, July and August) of the first cut of the season. The fungal load increased with later harvest dates in haylage, but fungal species detected in the herbage were not detected in the haylage. In the second study, bales from 124 farms were sampled, and data on production factors, chemical composition and mycotoxin presence included. Samples for analysis of fungi were taken from patches with visible fungal growth on the bale surfaces, and from drilled samples from the forage. Results showed a higher risk of fungal presence with increasing DM concentration, or if less than eight layers of polyethylene stretch film were used for wrapping. Presence of mycotoxins and their respective fungal species were not correlated (P>0.05). However, higher fungal counts were positively correlated with presence of mycotoxins. Ocular inspection and cultivation for identification of fungal species is timeand labour consuming and has inherent difficulties. Therefore, identification of fungal species by extracting fungal DNA directly from forage samples is of interest. A study on three new primers in the fungal ITS (internal transcribed spacer) region for 454-sequencing was performed. Results showed that not all fungal species can be identified in the ITS-region and therefore other DNA regions are of interest.

Subjects/Keywords: filamentous fungi; mould; wrapped forages; haylage and mycotoxins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schenck, J. (2019). Filamentous fungi in wrapped forages. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/16413/

Chicago Manual of Style (16^th Edition):

Schenck, Jessica. “Filamentous fungi in wrapped forages.” 2019. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed March 08, 2021. https://pub.epsilon.slu.se/16413/.

MLA Handbook (7^th Edition):

Schenck, Jessica. “Filamentous fungi in wrapped forages.” 2019. Web. 08 Mar 2021.

Vancouver:

Schenck J. Filamentous fungi in wrapped forages. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2019. [cited 2021 Mar 08]. Available from: https://pub.epsilon.slu.se/16413/.

Council of Science Editors:

Schenck J. Filamentous fungi in wrapped forages. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2019. Available from: https://pub.epsilon.slu.se/16413/

16. Sia, Eliandra de Freitas. Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta).

Degree: 2012, Universidade Federal do Amazonas

URL: http://tede.ufam.edu.br/handle/tede/3126

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Os fungos filamentosos são um importante grupo de micro-organismos, não apenas por serem os mais numerosos dentre todos os micro-organismos, mas devido ao seu potencial… (more)

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Os fungos filamentosos são um importante grupo de micro-organismos, não apenas por serem os mais numerosos dentre todos os micro-organismos, mas devido ao seu potencial em processos biotecnológicos como a produção de antibióticos e enzimas, fontes de vitaminas, alimentação e outros. Os fungos também são conhecidos como patógenos de plantas, animais e humanos, e produtores de toxinas. Cultivar fungos é uma rotina em laboratórios de microbiologia e um dos meios muito utilizado é o Batata Dextrose Ágar (BDA), que utiliza a batata como uma fonte rica em amido. Em algumas regiões do país, como a Região Norte, a batata tem um elevado custo e por este motivo a busca por novas fontes de amido é uma alternativa. Assim, outros vegetais devem ser pesquisados visando a substituição da batata. No presente trabalho foram inicialmente utilizados mandioca (Manihot esculenta), batata-doce (Ipomoea batatas), cenoura (Daucus carota), gengibre (Zingiber officinale) e cará (Dioscorea alata), sendo demonstrado que a mandioca (Manihot esculenta) é uma alternativa relevante para o cultivo de fungos. Esta espécie vegetal é uma planta tropical de grande importância comercial no Brasil, na África e em outras regiões do mundo, sendo que para a região Norte a mandioca é muito cultivada e também de baixo custo. Assim, após a triagem inicial com outros tubérculos, foi realizada a avaliação e otimização do meio de cultura de Mandioca Dextrose Ágar (MDA). Para o ensaio do meio com mandioca foram utilizadas diversas espécies de fungos com importância biotecnológica (Beauveria bassiana, Aspergillus niger, Penicillium sp., Fusarium sp., Phanerochaete chrysosporium). Foi avaliada a taxa de crescimento, a esporulação e a formação de hifas no meio de cultura BDA (utilizado como controle) e MDA. Os resultados comprovaram a eficiência do MDA para o crescimento dos fungos analisados, destacando-se a taxa de crescimento radial em meio sólido e peso seco do micélio em meio líquido. Entretanto, em MDA há uma menor esporulação dos fungos quando comparado ao BDA. Finalmente, foi realizado, utilizando meio BDA e MDA, o isolamento de fungos endofíticos de duas plantas: guaraná (Paullinia cupana) e oliveira (Olea europea), nos quais o meio de cultura MDA também se mostrou eficiente. A identificação de fungos endofíticos revelou que eles variaram conforme a planta hospedeira. A diversidade de fungos endofíticos foi maior em guaraná no meio MDA que em BDA, e o número de isolados foi menor em oliveira nos dois meios utilizados. Assim, do ponto de vista econômico e social, o substrato de mandioca pode ser considerado uma alternativa de alta potencialidade na obtenção de meios de cultura para laboratórios de micologia, especialmente em países tropicais

The filamentous fungi are an important group of microorganisms, being not only one of the most numerous among the microorganisms due to their potential in biotechnological processes such as the production of antibiotics, enzymes, vitamins and others. The fungi are also plant, animals and human pathogens, acting…

Advisors/Committee Members: Azevedo, João Lúcio de, CPF:04103262834, http://lattes.cnpq.br/2302429651674634, Pereira, José Odair, CPF:74268023887, http://lattes.cnpq.br/5559640659851194.

Subjects/Keywords: Mandioca; Fungos filamentosos; Endófitos; Filamentous fungi; Endophytes; CIENCIAS BIOLOGICAS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sia, E. d. F. (2012). Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta). (Doctoral Dissertation). Universidade Federal do Amazonas. Retrieved from http://tede.ufam.edu.br/handle/tede/3126

Chicago Manual of Style (16^th Edition):

Sia, Eliandra de Freitas. “Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta).” 2012. Doctoral Dissertation, Universidade Federal do Amazonas. Accessed March 08, 2021. http://tede.ufam.edu.br/handle/tede/3126.

MLA Handbook (7^th Edition):

Sia, Eliandra de Freitas. “Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta).” 2012. Web. 08 Mar 2021.

Vancouver:

Sia EdF. Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta). [Internet] [Doctoral dissertation]. Universidade Federal do Amazonas; 2012. [cited 2021 Mar 08]. Available from: http://tede.ufam.edu.br/handle/tede/3126.

Council of Science Editors:

Sia EdF. Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (manihot esculenta). [Doctoral Dissertation]. Universidade Federal do Amazonas; 2012. Available from: http://tede.ufam.edu.br/handle/tede/3126

17. Sanches, Michele Alves. Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica.

Degree: 2016, Universidade Federal do Amazonas

URL: http://tede.ufam.edu.br/handle/tede/5159

►

Human being has the need to mix water with insoluble substances in their daily and industrial activities. Surfactants derived from petroleum have been used for… (more)

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Human being has the need to mix water with insoluble substances in their daily and industrial activities. Surfactants derived from petroleum have been used for this purpose, however, they have problems such as their toxicity and biodegradability. Biosurfactants from microbial origin are an alternative to this, on the other hand, its production cost is high due to the substrates that are used in the bioprocesses. In this context, the aim of this study was to investigate the use of wastes as a substrate for the production of biosurfactants by filamentous fungi isolated from soil samples in Amazon region. Bioprocess were conducted with five known fungi isolates producers of biosurfactants in order to investigate which of them would produce in a shorter time. Then, we evaluated the possibility using wastes of peel from tucumã, peach palm, banana, cupuacu and also the post-fry oil as substrate for the bioprocess with the selected isolated. Factors that influence the production of biosurfactant were evaluated by factorial design. As a result, the fungus Fusarium oxysporum 87-2LIVI produced high levels of biosurfactants in three days of bioprocess. Moreover, this specie also showed highlight due to high biosurfactant production using peach palm peel as substract. The pH and yeast extract were the most important factors related to production of biosurfactant by this isolated. Thus, the present study showed isolated of F. oxysporum specie from Amazonian soil is potential producer of surfactants and also Amazon waste, particularly, peel of peach palm has the potential to be used as a bioprocess substrate for production of biosurfactant.

O homem tem a necessidade de misturar a água com substâncias insolúveis em suas atividades diárias e industriais. Os surfactantes, derivados de petróleo, têm sido utilizados com essa finalidade, no entanto, esses apresentam problemas quanto a sua toxicidade e biodegradabilidade. Os biossurfactantes de origem microbiana são uma alternativa para essa situação, porém, o seu custo de produção é alto, e em parte, devido aos substratos que são utilizados nos bioprocessos. Neste contexto, o objetivo deste trabalho foi investigar a utilização de resíduos como substrato para produção de biossurfactantes por fungos filamentosos isolados de amostras de solo da região Amazônica. Foram realizados bioprocessos com cinco isolados fúngicos sabidamente produtores de biossurfactantes, com a finalidade de investigar qual deles produziria em menor tempo. Em seguida, foi avaliada a possibilidade de utilização de resíduos (cascas de tucumã, pupunha, banana, cupuaçu e também o óleo pós-fritura) como subtrato para o bioprocesso com o isolado selecionado. Os fatores que influenciam na produção do biossurfactante foram avaliados por planejamento fatorial. O fungo Fusarium oxysporum 87-2LIVI produziu altos níveis de biossurfactantes em três dias de bioprocesso. E também se destacou devido a alta produção de biossurfactante utilizando a casca de pupunha como substrato. O pH e extrato de levedura foram…

Advisors/Committee Members: Souza, João Vicente Braga de, 63593955253, http://lattes.cnpq.br/7804981785557071.

Subjects/Keywords: Fungos filamentosos; Resíduos vegetais; Filamentous fungi; CIÊNCIAS DA SAÚDE: FARMÁCIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sanches, M. A. (2016). Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica. (Masters Thesis). Universidade Federal do Amazonas. Retrieved from http://tede.ufam.edu.br/handle/tede/5159

Chicago Manual of Style (16^th Edition):

Sanches, Michele Alves. “Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica.” 2016. Masters Thesis, Universidade Federal do Amazonas. Accessed March 08, 2021. http://tede.ufam.edu.br/handle/tede/5159.

MLA Handbook (7^th Edition):

Sanches, Michele Alves. “Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica.” 2016. Web. 08 Mar 2021.

Vancouver:

Sanches MA. Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica. [Internet] [Masters thesis]. Universidade Federal do Amazonas; 2016. [cited 2021 Mar 08]. Available from: http://tede.ufam.edu.br/handle/tede/5159.

Council of Science Editors:

Sanches MA. Utilização de resíduos vegetais para a produção de biossurfactantes por fungos isolados de amostras de solo da região amazônica. [Masters Thesis]. Universidade Federal do Amazonas; 2016. Available from: http://tede.ufam.edu.br/handle/tede/5159

University of North Carolina – Greensboro

18. El-Elimat, Tamam M. Discovering new structural diversity from unexplored fungi.

Degree: 2014, University of North Carolina – Greensboro

URL: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16161

► Discovery of anticancer drugs with high efficacy coupled with action at novel target sites is necessary to combat cancer. As part of a multidisciplinary project… (more)

Subjects/Keywords: Antineoplastic agents – Development; Medical mycology; Filamentous fungi – Therapeutic use

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

El-Elimat, T. M. (2014). Discovering new structural diversity from unexplored fungi. (Doctoral Dissertation). University of North Carolina – Greensboro. Retrieved from http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16161

Chicago Manual of Style (16^th Edition):

El-Elimat, Tamam M. “Discovering new structural diversity from unexplored fungi.” 2014. Doctoral Dissertation, University of North Carolina – Greensboro. Accessed March 08, 2021. http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16161.

MLA Handbook (7^th Edition):

El-Elimat, Tamam M. “Discovering new structural diversity from unexplored fungi.” 2014. Web. 08 Mar 2021.

Vancouver:

El-Elimat TM. Discovering new structural diversity from unexplored fungi. [Internet] [Doctoral dissertation]. University of North Carolina – Greensboro; 2014. [cited 2021 Mar 08]. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16161.

Council of Science Editors:

El-Elimat TM. Discovering new structural diversity from unexplored fungi. [Doctoral Dissertation]. University of North Carolina – Greensboro; 2014. Available from: http://libres.uncg.edu/ir/listing.aspx?styp=ti&id=16161

Macquarie University

19. Bali, Hana. Detection and identification of extracellular proteases in Trichoderma reesei.

Degree: 2013, Macquarie University

URL: http://hdl.handle.net/1959.14/267752

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"A thesis submitted in fulfilment of the requirements for the degree of Master of Philosophy, Department of Chemistry and Biomolecular Sciences, Macquarie University, Australia".

"October… (more)

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"A thesis submitted in fulfilment of the requirements for the degree of Master of Philosophy, Department of Chemistry and Biomolecular Sciences, Macquarie University, Australia".

"October 2012"

Includes bibiographical references.

1. Introduction – 2. Materials and methods – 3. Results and discussions – 4. Summary and future prospects.

"A major factor affecting the yield of heterologous protein production from filamentous fungi is the degradation of the target protein by proteases produced by the host organism. One approach to reduce this degradation by proteases is the identification of the proteases produced from the fungi followed by inactivation of the gene(s) encoding the harmful proteases. This project involved characterisation of the protease profile of three strains of the filamentous fungus Trichoderma reesei: QM6a (wild type), Rut-C30 (transformation host) and CVt (producing a heterologous Venus protein). Firstly, zymography was used to allow comparison of extracellular proteases produced from these three strains. Then, liquid assays using specific fluorescent substrates, and mass spectrometric analysis were used to identify the extracellular protease types secreted by the Rut-C30 and CVt strains. Both strains of T. reesei examined were found to produce extracellular proteases that were active across neutral to acidic pH range. Liquid protease activity assays using specific substrates and inhibitors for each protease type showed that there was a resemblance in the protease types which were secreted by T. reesei (Rut-C30 and CVt) strains. Aspartic proteases, chymotrypsin-like proteases and subtilisin-like proteases activities were detected by this method. However, there was higher aspartic protease activity in Rut-C30 supernatants whereas there was higher chymotrypsin-like protease and subtilisin-like protease activities in CVt supernatants. In addition, mass spectrometric analysis (MALDI TOF/TOF MS/MS and LC ESI MS/MS) showed both similarities and differences in the types of proteases between the two strains. Aspartic protease, chymotrypsin-like protease and subtilisin-like protease were identified from both strains. However, three proteases (carboxypeptidase, metallopeptidases and serine protease) were detected from the CVt strain only. This project paves the way for molecular engineering of protease deficient production hosts" – Abstract.

1 online resource (99 pages) illustrations

Advisors/Committee Members: Macquarie University. Dept. of Chemistry and Biomolecular Sciences.

Subjects/Keywords: Proteolytic enzymes; Trichoderma reesei – Molecular aspects; Proteases; filamentous fungi; zymography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bali, H. (2013). Detection and identification of extracellular proteases in Trichoderma reesei. (Masters Thesis). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/267752

Chicago Manual of Style (16^th Edition):

Bali, Hana. “Detection and identification of extracellular proteases in Trichoderma reesei.” 2013. Masters Thesis, Macquarie University. Accessed March 08, 2021. http://hdl.handle.net/1959.14/267752.

MLA Handbook (7^th Edition):

Bali, Hana. “Detection and identification of extracellular proteases in Trichoderma reesei.” 2013. Web. 08 Mar 2021.

Vancouver:

Bali H. Detection and identification of extracellular proteases in Trichoderma reesei. [Internet] [Masters thesis]. Macquarie University; 2013. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1959.14/267752.

Council of Science Editors:

Bali H. Detection and identification of extracellular proteases in Trichoderma reesei. [Masters Thesis]. Macquarie University; 2013. Available from: http://hdl.handle.net/1959.14/267752

University of Georgia

20. Shah, Punit. Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/25048

► Botrytis cinerea is a filamentous fungus infecting more than 200 plant species, causing significant economic losses worldwide. Filamentous fungi have been known to secrete enzymes… (more)

Subjects/Keywords: Botrytis; Filamentous fungi; Secretome; Mass spectrometry; Proteomics; Electrospray ionization; Liquid chromatography

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shah, P. (2014). Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/25048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shah, Punit. “Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions.” 2014. Thesis, University of Georgia. Accessed March 08, 2021. http://hdl.handle.net/10724/25048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shah, Punit. “Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions.” 2014. Web. 08 Mar 2021.

Vancouver:

Shah P. Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10724/25048.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shah P. Proteomics approach to study Botrytis cinerea secreted proteins under different growth conditions. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/25048

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

21. Cao, Yu. Towards a fundamental understanding of solid state bioprocessing.

Degree: PhD, 2019, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/towards-a-fundamental-understanding-of-solid-state-bioprocessing(6ca6322e-3e22-48a9-a19b-2cc624a4bc34).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.791238

► Solid state fermentation and solid state bioprocessing have been recognised for their unique features. However, there are several unsolved challenges which have prevented their widespread… (more)

▼ Solid state fermentation and solid state bioprocessing have been recognised for their unique features. However, there are several unsolved challenges which have prevented their widespread adoptions. In order to address these challenges, an investigation has been conducted, aiming at both identifying and addressing the problems. Two common applications of solid state bioprocessing, namely reducing toxins in raw materials and producing generic microbial feedstocks, were studied and are reported in Part A of the thesis (Chapter 3 and Chapter 4). The findings reported in Chapter 3 show that by applying a two stage solid state bioprocess, the major toxin in rapeseed meal can be reduced to a level suitable for animal consumption. The first stage, pre-incubation, greatly assisted toxin reduction in the second stage (fermentation), through the addition of water into the substrate. Although the exact mechanisms were not elucidated, it was clear that water is a critical factor in the process. In Chapter 4, a three stage solid state bioprocess was used to produce a generic microbial feedstock from mixed rapeseed meal and sugarcane bagasse. The main findings from this study were that such processes are highly variable, and that the performance of solid state fermentation is highly influenced by the microscopic environment of the microorganism. This led to the conclusion that a more fundamental understanding of the system is required. To obtain a better fundamental understanding, the effect of water on fungi cultivated on solid substrates (water bioavailability), was investigated in Part B of the thesis which includes theoretical and experimental investigations. The following theory has been developed from the findings. Fungal growth can only consume water from the surrounding local micro-environment. As germination and growth occur, they result in a modest depletion of water from the immediate vicinity. This creates a driving force for water to migrate from the bulk substrate (usually through diffusion), or to be absorbed from the gas phase, to replenish the water that has been consumed. The driving force within the substrate increases with fungal water consumption, while water absorption from the gas phase is driven both by the gas phase relative humidity and presence/absence of water at the substrate surface. Meanwhile, the resistance to mass transfer is greatly affected by both chemical and physical properties of the substrate. Fungal growth can continue, if, and only if water within their immediate vicinity can be replenished. Experiments showed that water bioavailability cannot be fully represented by terms such as 'water content' and 'water activity' but is affected by the presence of water in the immediate vicinity of the fungus. This is under the influence of substrate chemical properties and consequently affects germination and early growth. It is also affected by mobility of water through the substrate which impacts on growth rate in the absence of gas phase water. Further, if there is water in the gas phase, it can…

Subjects/Keywords: Microbial activity; Solid state fermentation; Water; Dynamic vapour sorption; filamentous fungi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cao, Y. (2019). Towards a fundamental understanding of solid state bioprocessing. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/towards-a-fundamental-understanding-of-solid-state-bioprocessing(6ca6322e-3e22-48a9-a19b-2cc624a4bc34).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.791238

Chicago Manual of Style (16^th Edition):

Cao, Yu. “Towards a fundamental understanding of solid state bioprocessing.” 2019. Doctoral Dissertation, University of Manchester. Accessed March 08, 2021. https://www.research.manchester.ac.uk/portal/en/theses/towards-a-fundamental-understanding-of-solid-state-bioprocessing(6ca6322e-3e22-48a9-a19b-2cc624a4bc34).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.791238.

MLA Handbook (7^th Edition):

Cao, Yu. “Towards a fundamental understanding of solid state bioprocessing.” 2019. Web. 08 Mar 2021.

Vancouver:

Cao Y. Towards a fundamental understanding of solid state bioprocessing. [Internet] [Doctoral dissertation]. University of Manchester; 2019. [cited 2021 Mar 08]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/towards-a-fundamental-understanding-of-solid-state-bioprocessing(6ca6322e-3e22-48a9-a19b-2cc624a4bc34).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.791238.

Council of Science Editors:

Cao Y. Towards a fundamental understanding of solid state bioprocessing. [Doctoral Dissertation]. University of Manchester; 2019. Available from: https://www.research.manchester.ac.uk/portal/en/theses/towards-a-fundamental-understanding-of-solid-state-bioprocessing(6ca6322e-3e22-48a9-a19b-2cc624a4bc34).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.791238

Oklahoma State University

22. Lightfoot, Jorge David. Metabolically Engineering Aspergillus nidulans for Client Protein Production.

Degree: Microbiology, Cell and Molecular Biology, 2019, Oklahoma State University

URL: http://hdl.handle.net/11244/323358

► Our silenced strains, when transformed to produce client proteins, have also had a marked change in the amount of protein produced, as well as how… (more)

Subjects/Keywords: aspergillus nidulans; cell factory; cellulase; enzyme production; filamentous fungi; rna interference

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APA (6^th Edition):

Lightfoot, J. D. (2019). Metabolically Engineering Aspergillus nidulans for Client Protein Production. (Thesis). Oklahoma State University. Retrieved from http://hdl.handle.net/11244/323358

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lightfoot, Jorge David. “Metabolically Engineering Aspergillus nidulans for Client Protein Production.” 2019. Thesis, Oklahoma State University. Accessed March 08, 2021. http://hdl.handle.net/11244/323358.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lightfoot, Jorge David. “Metabolically Engineering Aspergillus nidulans for Client Protein Production.” 2019. Web. 08 Mar 2021.

Vancouver:

Lightfoot JD. Metabolically Engineering Aspergillus nidulans for Client Protein Production. [Internet] [Thesis]. Oklahoma State University; 2019. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/11244/323358.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lightfoot JD. Metabolically Engineering Aspergillus nidulans for Client Protein Production. [Thesis]. Oklahoma State University; 2019. Available from: http://hdl.handle.net/11244/323358

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Johnston, Thomas. Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus.

Degree: 2012, NC Docks

URL: http://libres.uncg.edu/ir/uncg/f/Johnston_uncg_0154M_10827.pdf

► Part I: Adsorbent resins are used in industrial fermentations to help boost the production of antibiotics. The resins used here were utilized to help speed… (more)

Subjects/Keywords: Drug development; Antineoplastic antibiotics $x Development; Filamentous fungi $x Biotechnology

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APA (6^th Edition):

Johnston, T. (2012). Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/Johnston_uncg_0154M_10827.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johnston, Thomas. “Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus.” 2012. Thesis, NC Docks. Accessed March 08, 2021. http://libres.uncg.edu/ir/uncg/f/Johnston_uncg_0154M_10827.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johnston, Thomas. “Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus.” 2012. Web. 08 Mar 2021.

Vancouver:

Johnston T. Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus. [Internet] [Thesis]. NC Docks; 2012. [cited 2021 Mar 08]. Available from: http://libres.uncg.edu/ir/uncg/f/Johnston_uncg_0154M_10827.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johnston T. Use of adsorbent resins in fermentations of bacteria obtained from the red soils of the Kingdom of Jordan and the isolation and structure elucidation of cytotoxic xanthones from an unidentified fungus. [Thesis]. NC Docks; 2012. Available from: http://libres.uncg.edu/ir/uncg/f/Johnston_uncg_0154M_10827.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. El-Elimat, Tamam M. Discovering new structural diversity from unexplored fungi.

Degree: 2014, NC Docks

URL: http://libres.uncg.edu/ir/uncg/f/ElElimat_uncg_0154D_11383.pdf

► Discovery of anticancer drugs with high efficacy coupled with action at novel target sites is necessary to combat cancer. As part of a multidisciplinary project… (more)

Subjects/Keywords: Antineoplastic agents $x Development; Medical mycology; Filamentous fungi $x Therapeutic use

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

El-Elimat, T. M. (2014). Discovering new structural diversity from unexplored fungi. (Thesis). NC Docks. Retrieved from http://libres.uncg.edu/ir/uncg/f/ElElimat_uncg_0154D_11383.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

El-Elimat, Tamam M. “Discovering new structural diversity from unexplored fungi.” 2014. Thesis, NC Docks. Accessed March 08, 2021. http://libres.uncg.edu/ir/uncg/f/ElElimat_uncg_0154D_11383.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

El-Elimat, Tamam M. “Discovering new structural diversity from unexplored fungi.” 2014. Web. 08 Mar 2021.

Vancouver:

El-Elimat TM. Discovering new structural diversity from unexplored fungi. [Internet] [Thesis]. NC Docks; 2014. [cited 2021 Mar 08]. Available from: http://libres.uncg.edu/ir/uncg/f/ElElimat_uncg_0154D_11383.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

El-Elimat TM. Discovering new structural diversity from unexplored fungi. [Thesis]. NC Docks; 2014. Available from: http://libres.uncg.edu/ir/uncg/f/ElElimat_uncg_0154D_11383.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Macquarie University

25. Peterson, Robyn Anne. Enzyme activities of fungi isolated from koala faeces.

Degree: PhD, 2011, Macquarie University

URL: http://hdl.handle.net/1959.14/221878

►

Thesis by publication.

Bibliography: p. 184-211.

Introduction – Materials and methods – Isolation and identification of fungi from koala faeces and screening for their enzyme… (more)

▼

Thesis by publication.

Bibliography: p. 184-211.

Introduction – Materials and methods – Isolation and identification of fungi from koala faeces and screening for their enzyme activities of biotechnological interest – Cultivation of selected fungal isolates from koala faeces in liquid media for further enzyme characterisation – The secretome of Doratomyces stemonitis C8, isolated from koala faeces – Main findings and future prospects.

Filamentous fungi secrete enzymes to break down complex substances in the environment into smaller molecules they can use for nutrition. Investigation of the enzymes secreted by a fungus can lead to a better understanding of how it survives in a natural habitat; furthermore, new enzymes with potential for industrial applications can be revealed. In this work, the enzyme activities of fungi from koala faeces were investigated. As a result of a diet of Eucalyptus leaves, koala faeces are composed of recalcitrant plant cell wall polymers (cellulose, hemicellulose, pectin and lignin). Consequently, fungi that grow on koala faeces hold high potential as sources of enzymes for efficient degradation of plant biomass. – Thirty-seven fungal strains were isolated from koala faeces, identified, and screened for enzyme activities using agar plate assays; over two-thirds of the isolates secreted xylanases, endoglucanases, ligninases and proteases, and over one-third secreted amylases, mannanases and tannases. The enzyme activities of seven isolates were comprehensively characterised using liquid cultures, liquid enzyme assays and zymography. Two isolates, Gelasinospora cratophora A10 and Trichoderma atroviride A2, were high secretors of protein and heat-tolerant enzymes. The lipase(s) from Mariannaea camptospora A11 sustained activity at cool temperatures. The xylanase(s), mannanase(s), endoglucanase(s) and β-glucosidase(s) of Doratomyces stemonitis C8 displayed optimal activities under neutral to alkaline conditions. Some of the enzymes hold potential for application in the production of paper, textiles, detergents and ethanol-based biofuels. – Finally, the secretome of D. stemonitis C8 was studied by gel electrophoresis and mass spectrometry. As the genome of D. stemonitis has not been sequenced, the secretome analysis required cross-species identification and de novo sequencing; furthermore, a new technique was developed to identify proteins directly from zymogram gels by mass spectrometry. In the first secretome analysis of a coprophilous fungus, a complex array of enzymes integral to plant biomass degradation was identified, including enzymes that could be of value to industry in the future.

1 online resource (243 p.) col. ill

Advisors/Committee Members: Macquarie University. Dept. of Chemistry and Biomolecular Sciences.

Subjects/Keywords: Fungal enzymes; Fungi – Secretion; Filamentous fungi; Koala – Feces; Enzymes – Environmental aspects; Fungal molecular biology; corprophilous; fungi; enzyme; secretome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Peterson, R. A. (2011). Enzyme activities of fungi isolated from koala faeces. (Doctoral Dissertation). Macquarie University. Retrieved from http://hdl.handle.net/1959.14/221878

Chicago Manual of Style (16^th Edition):

Peterson, Robyn Anne. “Enzyme activities of fungi isolated from koala faeces.” 2011. Doctoral Dissertation, Macquarie University. Accessed March 08, 2021. http://hdl.handle.net/1959.14/221878.

MLA Handbook (7^th Edition):

Peterson, Robyn Anne. “Enzyme activities of fungi isolated from koala faeces.” 2011. Web. 08 Mar 2021.

Vancouver:

Peterson RA. Enzyme activities of fungi isolated from koala faeces. [Internet] [Doctoral dissertation]. Macquarie University; 2011. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/1959.14/221878.

Council of Science Editors:

Peterson RA. Enzyme activities of fungi isolated from koala faeces. [Doctoral Dissertation]. Macquarie University; 2011. Available from: http://hdl.handle.net/1959.14/221878

26. Wakiyama, Motoki. Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究.

Degree: 2009, University of Miyazaki / 宮崎大学

URL: http://hdl.handle.net/10458/1964

宮崎大学大学院工学研究科博士論文(平成20年度)

Subjects/Keywords: Xylan; Filamentous Fungi; β-Xylosidase; Xylanse

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wakiyama, M. (2009). Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究. (Thesis). University of Miyazaki / 宮崎大学. Retrieved from http://hdl.handle.net/10458/1964

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wakiyama, Motoki. “Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究.” 2009. Thesis, University of Miyazaki / 宮崎大学. Accessed March 08, 2021. http://hdl.handle.net/10458/1964.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wakiyama, Motoki. “Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究.” 2009. Web. 08 Mar 2021.

Vancouver:

Wakiyama M. Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究. [Internet] [Thesis]. University of Miyazaki / 宮崎大学; 2009. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10458/1964.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wakiyama M. Studies on Structure and Function of Xylanolytic Enzymes from Filamentous Fungi : 糸状菌由来のキシラン分解酵素の構造と機能に関する研究. [Thesis]. University of Miyazaki / 宮崎大学; 2009. Available from: http://hdl.handle.net/10458/1964

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

27. Zabala, Angelica Obusan. Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases.

Degree: Chemical Engineering, 2014, UCLA

URL: http://www.escholarship.org/uc/item/8g12b08v

► Polyketide synthases (PKSs) catalyze the production of important pharmaceuticals, including statins and antibiotics. Fungi are some of the most prolific producers of these compounds but… (more)

Subjects/Keywords: Chemical engineering; azaphilones; biosynthesis; brefeldin A; filamentous fungi; fungal PKS; natural product

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zabala, A. O. (2014). Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/8g12b08v

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zabala, Angelica Obusan. “Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases.” 2014. Thesis, UCLA. Accessed March 08, 2021. http://www.escholarship.org/uc/item/8g12b08v.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zabala, Angelica Obusan. “Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases.” 2014. Web. 08 Mar 2021.

Vancouver:

Zabala AO. Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases. [Internet] [Thesis]. UCLA; 2014. [cited 2021 Mar 08]. Available from: http://www.escholarship.org/uc/item/8g12b08v.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zabala AO. Bridging the Gap: Studying Sequence to Product Correlation among Fungal Polyketide Synthases. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/8g12b08v

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Riverside

28. Cabrera, Ilva Esther. The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa.

Degree: Genetics, Genomics and Bioinformatics, 2015, University of California – Riverside

URL: http://www.escholarship.org/uc/item/9sb284zq

► The G protein signaling pathway is very important in relaying information for growth and development. The main objectives of this dissertation are to: 1. Generate… (more)

▼ The G protein signaling pathway is very important in relaying information for growth and development. The main objectives of this dissertation are to: 1. Generate novel image processing tools with the aid of video bioinformatics, 2. Decipher the role of G protein signaling on development and growth through phenotypic analysis and localization studies, and 3. Confirm functions amongst proteins implicated in the Erk1/2 MAPK pathways in Neurospora crassa. In Chapter 2, I used video bioinformatics and image processing tools to develop algorithms that allowed for the analysis of vegetative cell compartment size, conidia size, and growth rate. I used the hyphal compartment size program and found that Δgna-1 and Δric8 had smaller length and diameter, whereas Δrgs-3, Δrgs-4, and Δrgs-5 had larger compartment sizes. Morphological assays were also conducted on the G signaling components. The results in Chapter 2, suggests that Δgna-1 and Δric8 regulate cell compartment size and growth rate in N. crassa. In Chapter 3, the phenotypic analysis of available G protein coupled receptor (GPCR) mutants were performed in a collaborative manner, with a majority of contributions by undergraduate students at UCR. All available GPCR mutants were phenotyped using classical morphological assays, as well as tested on chemicals for resistance or sensitivity on that tested chemical. Publically available gene expression data was obtained in order to compare expression patterns of the GPCR’s during growth and development. The results obtained in Chapter 3 improved our knowledge on GPCRs, especially on the Pth-11 related pathogenic receptors. In Chapter 4, the Gα subunits were fluorescently tagged, and their subcellular localization assayed during early development. In Chapter 4, my results demonstrated that all three Gα proteins localize to the plasma membrane in ungerminated conidia and young germlings, with GNA-1 also localizing on septa, and GNA-3 localizing on distinct patches on germlings. Lastly, Chapter 5 examined downstream effectors, specifically the MAK-1 and MAK-2, mitogen-activated protein kinase (MAPK) cascades in two G protein signaling component mutants, ric8 and ste50. The experiments revealed that ste50 is necessary for phosphorylation of MAK-2 in 16 hr VM liquid cultures and MAK-1 and MAK-2 in 6 day old VM plate cultures. However, MAK-1 and MAK-2 were phosphorylated to wild-type levels in cultures grown on low nitrogen medium (SCM) in Δste50 mutants. The objective of this dissertation was to shed light and expand the knowledge on G protein signaling components and their effects on growth and development in N. crassa.

Subjects/Keywords: Microbiology; Genetics; Molecular biology; Filamentous fungi; G protein signaling; Growth and Development; Neurospora crassa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cabrera, I. E. (2015). The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa. (Thesis). University of California – Riverside. Retrieved from http://www.escholarship.org/uc/item/9sb284zq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cabrera, Ilva Esther. “The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa.” 2015. Thesis, University of California – Riverside. Accessed March 08, 2021. http://www.escholarship.org/uc/item/9sb284zq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cabrera, Ilva Esther. “The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa.” 2015. Web. 08 Mar 2021.

Vancouver:

Cabrera IE. The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa. [Internet] [Thesis]. University of California – Riverside; 2015. [cited 2021 Mar 08]. Available from: http://www.escholarship.org/uc/item/9sb284zq.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cabrera IE. The Role of G Protein Signaling Components in Growth and Development of the Filamentous Fungus, Neurospora crassa. [Thesis]. University of California – Riverside; 2015. Available from: http://www.escholarship.org/uc/item/9sb284zq

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

29. Xu, Wei. Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids.

Degree: Chemical Engineering, 2013, UCLA

URL: http://www.escholarship.org/uc/item/6wm2q00r

► Filamentous fungi are known as promising sources for bio-active natural products, some of which are blockbuster drugs, such as penicillin and lovastatin. Fungal polyketide synthases… (more)

Subjects/Keywords: Chemical engineering; Biosynthesis; Filamentous fungi; Natural Product; Nonribosomal Peptide Synthetase; Polyketide Synthases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xu, W. (2013). Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/6wm2q00r

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xu, Wei. “Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids.” 2013. Thesis, UCLA. Accessed March 08, 2021. http://www.escholarship.org/uc/item/6wm2q00r.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xu, Wei. “Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids.” 2013. Web. 08 Mar 2021.

Vancouver:

Xu W. Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids. [Internet] [Thesis]. UCLA; 2013. [cited 2021 Mar 08]. Available from: http://www.escholarship.org/uc/item/6wm2q00r.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xu W. Mechanism Studies on Fungal Type I Highly-Reducing Polyketide Synthases and Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrids. [Thesis]. UCLA; 2013. Available from: http://www.escholarship.org/uc/item/6wm2q00r

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Missouri – Kansas City

30. El Mellouki, Tarik. Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1.

Degree: PhD, 2016, University of Missouri – Kansas City

URL: http://hdl.handle.net/10355/51184

► The motor proteins dynein and kinesin are fascinating biological machines which, like vehicles in a city, move various cellular cargoes along cytoskeletal microtubules (MT). In… (more)

Subjects/Keywords: Dynein; Kinesin; Filamentous fungi; Carrier proteins; Dissertation – University of Missouri – Kansas City – Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

El Mellouki, T. (2016). Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1. (Doctoral Dissertation). University of Missouri – Kansas City. Retrieved from http://hdl.handle.net/10355/51184

Chicago Manual of Style (16^th Edition):

El Mellouki, Tarik. “Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1.” 2016. Doctoral Dissertation, University of Missouri – Kansas City. Accessed March 08, 2021. http://hdl.handle.net/10355/51184.

MLA Handbook (7^th Edition):

El Mellouki, Tarik. “Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1.” 2016. Web. 08 Mar 2021.

Vancouver:

El Mellouki T. Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1. [Internet] [Doctoral dissertation]. University of Missouri – Kansas City; 2016. [cited 2021 Mar 08]. Available from: http://hdl.handle.net/10355/51184.

Council of Science Editors:

El Mellouki T. Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1. [Doctoral Dissertation]. University of Missouri – Kansas City; 2016. Available from: http://hdl.handle.net/10355/51184

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Open Access Theses and Dissertations