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University of Waterloo
1.
Anvari Naeini, Sina.
Computational Investigation of Role of Platelets In Cancer Metastasis.
Degree: 2020, University of Waterloo
URL: http://hdl.handle.net/10012/16583 
► Recent studies suggest that platelets have a significant role in improving the survival of circulating tumor cells and aggravating cancer metastasis. The main function of…
(more)
▼ Recent studies suggest that platelets have a significant role in improving the survival of circulating tumor cells and aggravating cancer metastasis. The main function of platelets, as a blood constituent, is to bind to the sites of the damaged vessels and stop bleeding. However, in cancer patients, activated platelets adhere to circulating cancer cells in the bloodstream and exacerbate the metastasis process in different ways. We hypothesize that: (1) platelets can protect circulating tumor cells from being destroyed by the blood flow due to large deformations and high shear stress; (2) platelets can form a protective layer around the circulating tumor cells that prevent the white blood cells from recognizing and destroying circulating tumor cells, increasing the survival rate of circulating tumor cells; (3) platelets enhance the extravasation of circulating tumor cells by increasing the number of adhesion bonds to the vessel wall and by secreting vascular endothelial growth factor that increases the permeability of vessels. These hypotheses have been stated several times in the literature, but the underlying mechanism of the platelet-cancer cell is still a mystery. Hence, in order to test these hypotheses and to find new therapeutic methods to reduce metastasis outcomes, we investigated the interactions between circulating tumor cells, blood flow, and platelets via computational modelling at the cellular scale. We used the Lattice Boltzmann method to simulate the plasma flow, the Discrete Element Method to model the deformation of the cells, and the Immersed Boundary Method to allow interactions between the plasma flow and deformable cells. We defined cell-cell and cell-vessel wall adhesion forces based on a stochastic adhesion model. Our highly detailed computational model helps us to understand and explain these phenomena and provides an effective tool to design and test new potential therapeutic methods based on platelet regulation.
Subjects/Keywords: platelets; metastasis; circulating tumor cell; adhesion; extravasation
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APA (6th Edition):
Anvari Naeini, S. (2020). Computational Investigation of Role of Platelets In Cancer Metastasis. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/16583
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Anvari Naeini, Sina. “Computational Investigation of Role of Platelets In Cancer Metastasis.” 2020. Thesis, University of Waterloo. Accessed January 19, 2021.
http://hdl.handle.net/10012/16583.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Anvari Naeini, Sina. “Computational Investigation of Role of Platelets In Cancer Metastasis.” 2020. Web. 19 Jan 2021.
Vancouver:
Anvari Naeini S. Computational Investigation of Role of Platelets In Cancer Metastasis. [Internet] [Thesis]. University of Waterloo; 2020. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10012/16583.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Anvari Naeini S. Computational Investigation of Role of Platelets In Cancer Metastasis. [Thesis]. University of Waterloo; 2020. Available from: http://hdl.handle.net/10012/16583
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Tichet, Mélanie.
Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2013, Nice
URL: http://www.theses.fr/2013NICE4150
► Le mélanome cutané est l'un des cancers les plus agressifs et mortel capables de dissémination métastatique à distance. L’intravasation cellules tumorales dans le vaisseau sanguin…
(more)
▼ Le mélanome cutané est l'un des cancers les plus agressifs et mortel capables de dissémination métastatique à distance. L’intravasation cellules tumorales dans le vaisseau sanguin dans les tumeurs primaires et l'extravasation sont des étapes importantes dans la formation de métastases. Ces étapes impliquent la perturbation de la barrière endothéliale par des cellules tumorales afin de faciliter leur migration transendothéliale et la colonisation métastatique. Cependant, le processus par lequel les cellules tumorales modulent l'intégrité des jonctions vasculaires est encore mal compris. Afin de déterminer les facteurs de perméabilité sécrétés par les cellules métastatiques, nous avons identifié la protéine matricielle SPARC comme un facteur critique contribuant à la perméabilité vasculaire et l'extravasation des cellules tumorales. Nous montrons que SPARC sécrété par les cellules de mélanome induit une perméabilité vasculaire via l'ouverture des jonctions intercellulaires des monocouches endothéliales et entraîne la migration transendothéliale de cellules de mélanome. In vivo, l’extinction de SPARC mène à une diminution drastique dans la colonisation à court et long terme des poumons et de la perméabilité des capillaires pulmonaires. A l’inverse, sa surexpression augmente les capacités d’extravasation et les métastases.
Cutaneous melanoma is one of the most aggressive cancers capable of distant and lethal metastatic spread. Tumor cell intravasation into blood vessel at primary tumor sites and subsequent extravasation are critical steps in the formation of metastases. These steps entail disruption of the endothelial barrier by tumor cells to facilitate their transendothelial passage and metastatic seeding. However, the way by which tumor cells modulate vascular junction integrity is still poorly understood. In an attempt to determine permeability factors secreted by metastatic cells, we identified the matricellular protein SPARC as a critical signaling factor that contributes to elevated vascular permeability and tumor cell extravasation. We show that SPARC released by melanoma cells enhances vascular leakiness by inducing opening of intercellular junctions of endothelial monolayers and drives melanoma cell transendothelial migration. In vivo vascular permeability and metastatic assays demonstrate that SPARC deficiency abrogates tumor-induced permeability of lung capillaries and prevents extravasation from blood vessels and metastasis, whereas overexpression of SPARC increases the lung metastatic potential of melanoma cells. Mechanistically, SPARC-induced endothelial gap formation and transmigration is dependent on vascular cell adhesion molecule (VCAM1) and p38 MAPK signaling pathway in endothelial cells. Importantly, blocking VCAM1 impedes melanoma cell extravasation. The clinical relevance of our findings is highlighted by the high levels of SPARC detected in tumor cells from human pulmonary melanoma lesions.
Advisors/Committee Members: Tartare-Deckert, Sophie (thesis director).
Subjects/Keywords: Mélanome; Métastase; SPARC; Extravasation; Perméabilité vasculaire; Melanoma; Metastasis; SPARC; Extravasation; Vascular permeability
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APA (6th Edition):
Tichet, M. (2013). Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination. (Doctoral Dissertation). Nice. Retrieved from http://www.theses.fr/2013NICE4150
Chicago Manual of Style (16th Edition):
Tichet, Mélanie. “Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination.” 2013. Doctoral Dissertation, Nice. Accessed January 19, 2021.
http://www.theses.fr/2013NICE4150.
MLA Handbook (7th Edition):
Tichet, Mélanie. “Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination.” 2013. Web. 19 Jan 2021.
Vancouver:
Tichet M. Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination. [Internet] [Doctoral dissertation]. Nice; 2013. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2013NICE4150.
Council of Science Editors:
Tichet M. Implication de la protéine matricielle SPARC dans la dissémination métastatique des mélanomes cutanés : Involvement of the matricellular protein SPARC in cutaneous melanoma metastatic dissemination. [Doctoral Dissertation]. Nice; 2013. Available from: http://www.theses.fr/2013NICE4150
Penn State University
3.
Haakenson, Jeremy Kenneth.
The Role of Ceramide in Metastatic Processes in Breast Cancer.
Degree: 2015, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/24221
► Ceramide is a bioactive sphingolipid that is capable of inducing apoptosis in mammalian cells. However, the role of ceramide in cancer metastasis remains largely unexplored.…
(more)
▼ Ceramide is a bioactive sphingolipid that is capable of inducing apoptosis in mammalian cells. However, the role of ceramide in cancer metastasis remains largely unexplored. In this dissertation, I have demonstrated that ceramide induces anoikis, inhibits
extravasation, and blocks the epithelial-mesenchymal transition (EMT) in metastasis-competent human breast cancer cell lines. Mechanistically, the effects of ceramide on anoikis and
extravasation are mediated by lysosomal degradation of CD44, independent of palmitoylation or proteasome targeting. SiRNA down-regulation of CD44 mimics ceramide-induced anoikis and diminished
extravasation of cancer cells. On the other hand, the ability of ceramide to prevent IL-6-induced EMT is dependent on its ability to inhibit STAT3 activation. Taken together, the data in this dissertation indicate that ceramide limits CD44-dependent breast cancer cell migration and IL-6/STAT3-dependent EMT, suggesting that ceramide analogs could be used to prevent and treat solid tumor metastasis.
Advisors/Committee Members: Mark Kester, Dissertation Advisor/Co-Advisor, Mark Kester, Committee Chair/Co-Chair, Andrea Manni, Dissertation Advisor/Co-Advisor, Andrea Manni, Committee Chair/Co-Chair, Gary Alan Clawson, Committee Member, Cheng Dong, Committee Member, Rosalyn Bryson Irby, Committee Member.
Subjects/Keywords: ceramide; anoikis; metastasis; breast cancer; CD44; extravasation; carcinoma; epithelial-mesenchymal transition
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APA (6th Edition):
Haakenson, J. K. (2015). The Role of Ceramide in Metastatic Processes in Breast Cancer. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/24221
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Haakenson, Jeremy Kenneth. “The Role of Ceramide in Metastatic Processes in Breast Cancer.” 2015. Thesis, Penn State University. Accessed January 19, 2021.
https://submit-etda.libraries.psu.edu/catalog/24221.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Haakenson, Jeremy Kenneth. “The Role of Ceramide in Metastatic Processes in Breast Cancer.” 2015. Web. 19 Jan 2021.
Vancouver:
Haakenson JK. The Role of Ceramide in Metastatic Processes in Breast Cancer. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Jan 19].
Available from: https://submit-etda.libraries.psu.edu/catalog/24221.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Haakenson JK. The Role of Ceramide in Metastatic Processes in Breast Cancer. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/24221
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Western Ontario
4.
Goertzen, Cameron G-F.
KISS1R Signaling Promotes Breast Cancer Metastasis.
Degree: 2014, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/2104
► Kisspeptins, peptide products of KISS1, are endogenous ligands for KISS1R, a G protein-coupled receptor. In numerous cancers, KISS1 acts as a metastasis suppressor. However, studies…
(more)
▼ Kisspeptins, peptide products of KISS1, are endogenous ligands for KISS1R, a G protein-coupled receptor. In numerous cancers, KISS1 acts as a metastasis suppressor. However, studies have revealed that patients with elevated KISS1 and KISS1R breast tumor expression have increased tumor grade, increased lymph node metastases and poor survival. We hypothesize that depletion of KISS1R inhibits breast cancer cell metastasis. In order to assess the role of KISS1R in breast cancer metastasis, we used a pre-clinical orthotopic xenograft mouse model using MDA-MB-231 breast cancer cells for breast tumor establishment. We discovered that depletion of KISS1R decreased primary tumor growth and reduced lung metastatic burden, suggesting that KISS1R plays a role in promoting breast cancer metastasis. Furthermore, we observed that kisspeptin-10 stimulation increased breast cancer cell invadopodia formation via a β-arrestin2 dependent mechanism. Overall, our results suggest that KISS1R may be a novel therapeutic target in the prevention of breast cancer metastasis.
Subjects/Keywords: KISS1R; Breast Cancer; Metastasis; Invadopodia; Extravasation; β-arrestin2; Cancer Biology
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APA (6th Edition):
Goertzen, C. G. (2014). KISS1R Signaling Promotes Breast Cancer Metastasis. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/2104
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Goertzen, Cameron G-F. “KISS1R Signaling Promotes Breast Cancer Metastasis.” 2014. Thesis, University of Western Ontario. Accessed January 19, 2021.
https://ir.lib.uwo.ca/etd/2104.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Goertzen, Cameron G-F. “KISS1R Signaling Promotes Breast Cancer Metastasis.” 2014. Web. 19 Jan 2021.
Vancouver:
Goertzen CG. KISS1R Signaling Promotes Breast Cancer Metastasis. [Internet] [Thesis]. University of Western Ontario; 2014. [cited 2021 Jan 19].
Available from: https://ir.lib.uwo.ca/etd/2104.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Goertzen CG. KISS1R Signaling Promotes Breast Cancer Metastasis. [Thesis]. University of Western Ontario; 2014. Available from: https://ir.lib.uwo.ca/etd/2104
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Follain, Gautier.
Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination.
Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2019, Université de Strasbourg
URL: http://www.theses.fr/2019STRAJ047
► Les cellules tumorales circulantes (CTCs) que l’on retrouve dans le sang des patients atteints de cancers sont responsables de la formation de métastases dans des…
(more)
▼ Les cellules tumorales circulantes (CTCs) que l’on retrouve dans le sang des patients atteints de cancers sont responsables de la formation de métastases dans des organes vitaux. Pendant ma thèse, nous avons travaillé sur l’étude du rôle de facteurs biomécaniques (tels que le flux sanguin, la force des récepteurs d’adhésion et les contraintes physiques) sur la dissémination des CTCs. En particulier, nous avons pu démontrer le rôle central des forces du flux sanguin dans les étapes d’arrêt, d’adhésion et d’extravasation des CTCs qui précèdent directement la formation des métastases. Nous avons mis en lumière un mécanisme d’adhésion des CTCs en deux étapes in vivo, faisant intervenir plusieurs types de récepteurs d’adhésion et la matrice extracellulaire. Enfin, nous avons mis en évidence la possibilité de bloquer le mécanisme d’extravasation par remodelage de l’endothélium dépendant du flux sanguin autour des cellules tumorales arrêtés dans les vaisseaux. Ceci ouvre la voie à de nouvelles stratégies thérapeutiques pour bloquer la progression tumorale.
Blood from patients with cancers contains circulating tumor cells (CTCs) that are responsible for the development of metastases in vital organs. During my PhD, we studied the role of biomechanical factors (such as blood flow, strengths of adhesion receptors and physical constraints) on the dissemination of CTCs. Especially, we demonstrated the central role of blood flow forces during the arrest, the adhesion and the extravasation of CTCs, preceding the metastatic outgrowth. We highlighted a two-step mechanism of CTCs adhesion, based on several adhesion receptors and the extracellular matrix. Finally, we successfully inhibited the extravasation of CTCs, by blocking the flow-dependent endothelial remodeling around arrested tumor cells in the vessels. This paves the way to establish new therapeutic strategies to block cancer progression.
Advisors/Committee Members: Goetz, Jacky (thesis director).
Subjects/Keywords: Métastases; Remodelage endothélial; Extravasation; Flux sanguin; Remodelage endothélial; Cellules tumorales circulantes; Metastasis; Circulating tumor cells; Extravasation; Blood flow; Endothelial remodeling; 571.4; 616.15; 616.99
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APA ·
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APA (6th Edition):
Follain, G. (2019). Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2019STRAJ047
Chicago Manual of Style (16th Edition):
Follain, Gautier. “Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination.” 2019. Doctoral Dissertation, Université de Strasbourg. Accessed January 19, 2021.
http://www.theses.fr/2019STRAJ047.
MLA Handbook (7th Edition):
Follain, Gautier. “Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination.” 2019. Web. 19 Jan 2021.
Vancouver:
Follain G. Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2019. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2019STRAJ047.
Council of Science Editors:
Follain G. Les forces hémodynamiques contrôlent la dissémination des cellules tumorales circulantes : Hemodynamic forces control circulating tumor cells dissemination. [Doctoral Dissertation]. Université de Strasbourg; 2019. Available from: http://www.theses.fr/2019STRAJ047
6.
Brunherotti, Mariana Ribeiro.
Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura.
Degree: Mestrado, Enfermagem Fundamental, 2007, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-13082007-102108/
;
► Os pacientes submetidos ao tratamento antineoplásico necessitam de um acesso venoso que permita a infusão segura das drogas quimioterápicas, evitando assim o risco do extravasamento.…
(more)
▼ Os pacientes submetidos ao tratamento antineoplásico necessitam de um acesso venoso que permita a infusão segura das drogas quimioterápicas, evitando assim o risco do extravasamento. O extravasamento quimioterápico é definido como o escape de drogas do vaso sanguíneo para os tecidos circunjacentes, e seus efeitos tóxicos locais variam podendo causar dor, necrose tissular ou descamação do tecido. A morbidade depende do tipo da droga, da quantidade extravasada, da sua concentração, da localização do extravasamento, das condições do paciente e do intervalo entre o fato, seu reconhecimento e o tratamento. A prática baseada em evidências é uma abordagem que capacita os profissionais buscarem a melhor evidência para o cuidado, respeitando a opinião dos pacientes e seus familiares. O presente estudo é uma revisão integrativa da literatura, que teve como objetivo buscar e avaliar as evidências disponíveis na literatura sobre as intervenções eficazes frente ao extravasamento de drogas quimioterápicas vesicantes, em cateteres periféricos, prevenindo e minimizando lesões no paciente adulto oncológico. Para a seleção dos artigos utilizamos a base de dados Medline, e a amostra constituiui-se de 16 artigos. As medidas de prevenção do extravasamento são consideradas mais eficazes e recomendadas. Para o manejo do extravasamento das drogas doxorrubicina e epirrubicina é recomendado aplicação de gelo local, os antídotos dimetilsulfoxide tópico e dexrazoxane intravenoso, intervenção cirúrgica se houver persistência dos sintomas ou para grande quantidade de droga extravasada. Para o extravasamento de mitomicina C, gelo local e intervenção cirúrgica em lesões detectadas após 48 horas; para mecloretamina é indicado gelo e o antídoto tiossulfato de sódio. Para o manejo da vinorelbine é recomendado calor local e o antídoto hialuronidase por via subcutânea. As evidências extraídas dos estudos analisados podem auxiliar a implementação de cuidados de enfermagem eficazes relacionados ao extravasamento das drogas vesicantes contribuindo assim com a melhoria da assistência à saúde.
Patients submitted to antineoplastic chemotherapy need a venous access that allows for the safe infusion of chemotherapeutic drugs, thus avoiding the risk of chemotherapeutic extravasation. Chemotherapeutic extravasation is defined as the escape of drugs from the blood vessel to surrounding tissues. Its local toxic effects vary and can cause pain, tissue necrosis or flaking. Morbidity depends on the type of drug, the extravasated quantity, its concentration, the location of the extravasation, the patient s conditions and the interval between the fact and its recognition and treatment. Evidence-based practice is an approach that trains professionals to look for the best evidence for care, respecting the opinions of patients and their relatives. This study is an integrative literature review that aimed to look for and assess available evidence in literature about effective interventions in case of extravasation of vesicant chemotherapeutic drugs, in peripheral…
Advisors/Committee Members: Sawada, Namie Okino.
Subjects/Keywords: Cuidados de Enfermagem; Extravasamento; Extravasation; Nursing Care; Quimioterápicos Vesicantes; Vesicant Chemotherapeutic agents
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APA ·
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APA (6th Edition):
Brunherotti, M. R. (2007). Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/22/22132/tde-13082007-102108/ ;
Chicago Manual of Style (16th Edition):
Brunherotti, Mariana Ribeiro. “Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura.” 2007. Masters Thesis, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/22/22132/tde-13082007-102108/ ;.
MLA Handbook (7th Edition):
Brunherotti, Mariana Ribeiro. “Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura.” 2007. Web. 19 Jan 2021.
Vancouver:
Brunherotti MR. Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura. [Internet] [Masters thesis]. University of São Paulo; 2007. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-13082007-102108/ ;.
Council of Science Editors:
Brunherotti MR. Intervenções no extravasamento de quimioterápicos vesicantes: revisão integrativa da literatura. [Masters Thesis]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-13082007-102108/ ;
University of Toronto
7.
Bergevin, Michele.
Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi.
Degree: 2017, University of Toronto
URL: http://hdl.handle.net/1807/77761
► Blood-borne bacteria like the Lyme disease pathogen Borrelia burgdorferi cause infection by migrating across the vascular endothelial barrier into target tissues. The mechanisms by which…
(more)
▼ Blood-borne bacteria like the Lyme disease pathogen Borrelia burgdorferi cause infection by migrating across the vascular endothelial barrier into target tissues. The mechanisms by which this occurs are poorly understood, largely because model systems inadequately mimic the in vivo environment or are too inefficient to dissect mechanisms. This unmet need is addressed in this thesis by the development of a microfluidic system and live cell imaging methods to model and study transendothelial migration of bacteria in vitro under physiologically relevant conditions. Real-time transmigration kinetics of B. burgdorferi across intact endothelium were obtained, for the first time, under static and flow conditions. Validation studies confirmed that B. burgdorferi transmigrate actively, with similar kinetics to conventional Transwell systems under static conditions. Additionally, physiological shear stress conditions appeared not to significantly alter transmigration kinetics. These data were uniquely obtainable with the microfluidic platform, supporting its utility for studying extravasation of blood-borne pathogens of worldwide significance.
M.A.S.
Advisors/Committee Members: Simmons, Craig A, Moriarty, Tara J, Mechanical and Industrial Engineering.
Subjects/Keywords: blood-borne bacteria; extravasation modeling; microfluidics; real time fluorescence microscopy; shear stress; transendothelial migration; 0648
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APA (6th Edition):
Bergevin, M. (2017). Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/77761
Chicago Manual of Style (16th Edition):
Bergevin, Michele. “Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi.” 2017. Masters Thesis, University of Toronto. Accessed January 19, 2021.
http://hdl.handle.net/1807/77761.
MLA Handbook (7th Edition):
Bergevin, Michele. “Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi.” 2017. Web. 19 Jan 2021.
Vancouver:
Bergevin M. Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1807/77761.
Council of Science Editors:
Bergevin M. Development and Characterization of a Microfluidic System to Model the Transendothelial Migration Mechanism of the Lyme Disease Pathogen Borrelia burgdorferi. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/77761
University of Adelaide
8.
Williams, Samantha Louise.
A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration.
Degree: 2009, University of Adelaide
URL: http://hdl.handle.net/2440/56715
► Neutrophil extravasation into tissues is an essential process required for the inflammatory response. Upon receiving an inflammatory cue, neutrophils begin accumulating on the luminal surface…
(more)
▼ Neutrophil
extravasation into tissues is an essential process required for the inflammatory response. Upon receiving an inflammatory cue, neutrophils begin accumulating on the luminal surface of the endothelium. Neutrophil recruitment is initiated by selectin-mediated tethering and rolling of neutrophils along the endothelial monolayer, followed by integrin-mediated firm adhesion. Adherent neutrophils then traverse the endothelium in a process known as transendothelial migration. The events mediating the rolling and adhesion steps are well characterised, but research into the molecular mechanisms regulating transendothelial migration is an area of intense focus. A previous study conducted in our laboratory found that the activation of endothelial extracellular signal-regulated kinase (ERK) 1/2 was required for neutrophil transmigration. Furthermore, it was found that endothelial ERK was activated in response to a soluble protein produced by fMLP- or IL-8-stimulated neutrophils.
In the present study, the soluble ERK-activating neutrophil protein was identified as annexin A1, which was selected as a possible candidate following mass spectrometry analysis of proteins secreted from activated neutrophils. Annexin A1 antibodies (Abs) were found to block endothelial ERK activation induced by conditioned medium harvested from stimulated neutrophils. Annexin A1 Abs were additionally able to inhibit neutrophil transmigration across human umbilical vein endothelial cell (HUVEC) monolayers in an in vitro transmigration assay. Following the purification of recombinant annexin A1, it was demonstrated that it could activate endothelial ERK in a similar manner to neutrophil conditioned medium. Upon further investigation, ERK activation was found to be induced by a truncated form of annexin A1 present in the protein preparation rather than the full length protein. Calpain I, a calcium dependent protease that is activated upon neutrophil stimulation and is known to cleave annexin A1 within the N-terminal domain, was shown to process full length inactive recombinant annexin A1 into an unidentified product that could activate endothelial ERK. A calpain I inhibitor was also found to prevent stimulated neutrophils from secreting an ERK-activating protein, thus further suggesting a role for calpain I in this process. As full length annexin A1 has been reported to signal through the formyl peptide receptor (FPR) family, a pan-FPR antagonist was incubated with endothelial cells and was found to inhibit ERK activation induced by neutrophil conditioned medium, indicating that pro-inflammatory annexin A1 is also a FPR ligand.
Endothelial projections termed “transmigratory cups” form around neutrophils during
extravasation, of which ICAM-1 is a major component. Using an assay that examined transmigratory cups during neutrophil transmigration, it was found that annexin A1 Abs could inhibit neutrophil adhesion and transmigration through HUVEC monolayers by interfering with transmigratory cup formation around neutrophils, as shown by monitoring…
Advisors/Committee Members: Khew-Goodall, Yeesim (advisor), Pitson, Stuart Maxwell (advisor), School of Molecular and Biomedical Science : Biochemistry (school).
Subjects/Keywords: neutrophil; transendothelial; migration; annexin A1; extravasation
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APA (6th Edition):
Williams, S. L. (2009). A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/56715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Williams, Samantha Louise. “A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration.” 2009. Thesis, University of Adelaide. Accessed January 19, 2021.
http://hdl.handle.net/2440/56715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Williams, Samantha Louise. “A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration.” 2009. Web. 19 Jan 2021.
Vancouver:
Williams SL. A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration. [Internet] [Thesis]. University of Adelaide; 2009. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/2440/56715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Williams SL. A novel proinflammatory role for annexin A1 in neutrophil transendothelial migration. [Thesis]. University of Adelaide; 2009. Available from: http://hdl.handle.net/2440/56715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
9.
Kong, Janet.
Intravenous Extravasation Management.
Degree: MSN, 2015, University of San Francisco
URL: https://repository.usfca.edu/capstone/170
► The purpose and intention of the project is to increase awareness in the topic of reducing intravenous extravasations. An assessment of my microsystem was…
(more)
▼ The purpose and intention of the project is to increase awareness in the topic of reducing intravenous extravasations. An assessment of my microsystem was completed that helped me identified the unit’s need for IV management safety. The problem assessed showed that approximately fifteen percent of intravenous catheters were infiltrated by the time patients arrived to pre-op surgery. The clinical setting took place at Huntington Hospital in Pasadena. This is a level 2-trauma center in the San Gabriel Valley with 625 beds in hospital to accommodate a diverse patient population. This project took place at a 32-bed in-patient Surgical Unit within the hospital. The method of analysis used for this project involved a pre-audit and post-audit tool to assess for IV compliance safety. Pre in-service data showed infiltration rates were at 4% while other significant variables such as IV tubing not in use remains sterile were under 65% and intact dressing were below 80%. An attendance of 42 nurses to a staff meeting was held to provide education on intravenous protocols. Teaching aid visuals and handouts were distributed to staff for reference. Post in-service audits were used to determine effectiveness of teaching. The implementation results showed a decreased by 2% on IV infiltrations while maintenance on keeping tubing sterile rose to 70%. Intact dressings were above 90% compliance. A success in decreasing in decreasing IV infiltrations and extravasations were reduced by 50%. The evaluation results concluded that the in-service provided to staff RNs showed improvement and will continue to be evaluated in the future.
Advisors/Committee Members: Elena Capella, Karin Blais.
Subjects/Keywords: intravenous management; extravasation; intravenous complications
…7
Running head: INTRAVENOUS EXTRAVASATION MANAGEMENT
Alekseyev, S., Byrne, M., Carpenter… …the article, the pathogenesis of
extravasation, types, symptoms, and evidence-based… …extravasation were discussed along with
issues related to peripheral and central venous catheters… …treatment of choice. Training along with applying
8
Running head: INTRAVENOUS EXTRAVASATION… …hopefully increase nursing awareness on intravenous management and decrease extravasation…
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APA (6th Edition):
Kong, J. (2015). Intravenous Extravasation Management. (Thesis). University of San Francisco. Retrieved from https://repository.usfca.edu/capstone/170
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kong, Janet. “Intravenous Extravasation Management.” 2015. Thesis, University of San Francisco. Accessed January 19, 2021.
https://repository.usfca.edu/capstone/170.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kong, Janet. “Intravenous Extravasation Management.” 2015. Web. 19 Jan 2021.
Vancouver:
Kong J. Intravenous Extravasation Management. [Internet] [Thesis]. University of San Francisco; 2015. [cited 2021 Jan 19].
Available from: https://repository.usfca.edu/capstone/170.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kong J. Intravenous Extravasation Management. [Thesis]. University of San Francisco; 2015. Available from: https://repository.usfca.edu/capstone/170
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
10.
Chen, Yu-Chung.
Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P.
Degree: Master, Biological Sciences, 2002, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723102-212041
► ãAbstractã Neurogenic inflammation is an acute inflammatory tissue response, that is mediated by sensory axon reflex. Accompanied with neurogenic inflammation, plasma extravasation, occurs in the…
(more)
▼ ãAbstractã
Neurogenic inflammation is an acute inflammatory tissue response, that is mediated by sensory axon reflex. Accompanied with neurogenic inflammation, plasma
extravasation, occurs in the eyes, esophagus, bladder, joints, the tip of tongue, and the respiratory tract of the mammal. Recently, many studies have investigated the neurogenic inflammation by electrical stimulation of nerves and intravascular injection of irritants. Upon stimulation, the sensory nerve endings in mucosa can release neuropeptides such as substance P, that causes formation of the venular endothelial gaps, plasma
extravasation and tissue edema in various organs. Substance P also cause smooth muscle contraction and mucus secretion in the respiratory tract.
Neurogenic plasma
extravasation has been studied extensively in the trachea, and bronchi, but rarely in the esophagus. It is known that a plexus of substance P-immunoreactive axons exists in the mucosal and submucosal layers. They play an important role in releasing substance P to act on the receptors of the venular endothelium through diffusion.
Based on plasma
extravasation and other studies related to the respiratory tract, the purpose of the present study was to investigate neurogenic inflammatory response in the esophagus of the digestive tract. In this study, capsaicin (90 µg/ml/kg) and substance P (3 µg/ml/kg) were used as the irritant and inflammatory mediator, respectively to reduce neurogenic inflammation in the esophagus. India ink was used to label the affected venules. The magnitude of the neurogenic inflammation was expressed as area density of India ink-labeled leaky venules. Histopathological changes in the esophageal tissue were studied under the light microscope.
The result of this study indicated that capsaicin at the dose of (90 µg/ml/kg) and substance P at the dose of (3 µg/ml/kg) caused similar magnitude of inflammation in the esophagus. India ink-labeled venules distributed like a network in the mucosal tissue and in connective tissue of the submucosal layer. The upper, middle and lower parts of esophagus exhibited the same degree of inflammatory response, that was similar to that in the lower respiratory tract as the previous studies reported. These results suggest that nerve branches from the vagal trunk send sensory axons to innervate both the esophagus and airways.
Advisors/Committee Members: Sheng-Nan Wu (chair), Hung-Tu Huang (committee member), Chen-Fu Shaw (chair).
Subjects/Keywords: capsaicin; esophagus; neurogenic plasma extravasation; venules; substance P
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, Y. (2002). Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723102-212041
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Yu-Chung. “Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P.” 2002. Thesis, NSYSU. Accessed January 19, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723102-212041.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Yu-Chung. “Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P.” 2002. Web. 19 Jan 2021.
Vancouver:
Chen Y. Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P. [Internet] [Thesis]. NSYSU; 2002. [cited 2021 Jan 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723102-212041.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen Y. Study on the Rat Esophageal Microcirculation that Mediated Inflammatory Response Evoked by Capsaicin and Substance P. [Thesis]. NSYSU; 2002. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723102-212041
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
11.
Chang, Jui-Hsin.
Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea.
Degree: Master, Biological Sciences, 2004, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714104-102314
► Many factors influence the inflammatory responses in rat trachea, including inflammatory mediators released from nerve fibers, histamine released by mast cells and endotoxin from the…
(more)
▼ Many factors influence the inflammatory responses in rat trachea, including inflammatory mediators released from nerve fibers, histamine released by mast cells and endotoxin from the cell walls of bacteria. The inflammatory responses include plasma
extravasation, subepithelial edema, and hypersecretion of secretory cells. But the mechanism of inflammation mediated by these factors was not completely understood. In the present study, a high dose of histamine was administered intravenously to induce the inflammation in rat airway. India ink was also injected as a tracer to label the leaky blood vessels in different time points. To investigate the serous cell secretion and subepithelial edema formation, the treacheal tissue was processed for histological study. Electron microcopy was carried out to investigate the ultrastructure of serous cells. To investigate the mechanism of histamine effect, the muscarinic receptor antagonist atropine (1 mg/ml/kg) or histamine H1 receptor antagonist mepyramine (10 mg/ml/kg) was injected 15 min before histamine injection. Five minutes after histamine, plasma leakage and serous cell secretion were extensive. The area density of India ink-labeled leaky vessels was 17.24 % ± 2.03 %. Saline, the vehicle of histamine, produced only a little
extravasation. Mepyramine inhibited the histamine-induced plasma
extravasation and serous cell degranulation significantly but atropine had no effect. The results suggest that histamine-induced serous cell degranulation is mainly through histamine H1 receptors but not through cholinergic muscarinic receptors in rat trachea.
Advisors/Committee Members: Hung-Tu Huang (committee member), Jiin-Tsuey Cheng (chair), Ching-Jiunn Tseng (chair).
Subjects/Keywords: trachea; histamine; plasma extravasation; inflammation; serous cell
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APA ·
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APA (6th Edition):
Chang, J. (2004). Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714104-102314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chang, Jui-Hsin. “Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea.” 2004. Thesis, NSYSU. Accessed January 19, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714104-102314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chang, Jui-Hsin. “Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea.” 2004. Web. 19 Jan 2021.
Vancouver:
Chang J. Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea. [Internet] [Thesis]. NSYSU; 2004. [cited 2021 Jan 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714104-102314.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chang J. Effect and mechanism of atropine and mepyramine on histamine-induced plasma leakage and serous cell secretion in the rat trachea. [Thesis]. NSYSU; 2004. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0714104-102314
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Auler e Salles, Murilo.
Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM).
Degree: PhD, Reabilitação Oral, 2011, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-14122011-091248/
;
► Busca-se em pesquisas e estudos avaliar a capacidade de adaptação e selamento entre a conexão implante/intermediário de diferentes sistemas de implantes odontológicos. Observou-se recentemente que…
(more)
▼ Busca-se em pesquisas e estudos avaliar a capacidade de adaptação e selamento entre a conexão implante/intermediário de diferentes sistemas de implantes odontológicos. Observou-se recentemente que implantes com abutments retidos com parafusos, diversos fenômenos como afrouxamento e fratura do parafuso, rotação e fratura do abutment com penetração bacteriana nas câmaras internas dos implantes, acontece como conseqüência da desadaptação interface implante/abutment. É descrito ao nível desta região um pequeno espaço microgap, fator relevante para remodelamento da crista óssea e longevidade da saúde dos tecidos moles periimplantares. O propósito do estudo foi investigar o extravasamento da solução do corante azul de Evan em três tipos de implantes e seus respectivos intermediários, durante um período de seis (6) dias, a cada vinte e quatro (24) horas, com intervalo em cento e vinte (120) horas, através da agitação proporcionada por uma mesa agitadora. Para tal, foram utilizados trinta (30) implantes, dez (10) de cada tipo, com seus respectivos intermediários protéticos, minipilares, sendo o Grupo Um (1) de implantes Hexágono Externo (HE), Grupo dois (2) de Hexágono Interno (HI) e Grupo três (3) de Cone Morse (CM). No interior de cada implante foi pipetado volume ou quantidade proporcional ao seu espaço interno uma solução de corante azul de Evan. Após a colocação do corante no interior dos implantes, os abutments ou intermediários foram acoplados e aparafusados com torque de vinte (20) Ncm, através do torquímetro de Gauge (Tohnichi), e estes depositados individualmente em micro tubos de cor âmbar na condição de intermediários voltados para baixo. Segui/se imediatamente a colocação de (1)ml de água deionizada. A seguir os tubos foram fechados hermeticamente e posicionados numa mesa suporte para microtubos e foram armazernados por 24 horas, sem agitação. Posteriormente foram agitados por 10 minutos com movimentos uniformes em mesa agitadora e a partir deste momento iniciou/se a coleta de uma pequena quantidade de água de cada micro tubo onde por sua vez estas amostras foram analisadas por absorbância através do método de fotometria, espectrofotometria, onde mostraram o extravasamento da solução do corante azul de Evan nos sistemas de implantes usados. Do inicio da coleta das amostras no tempo de (24 horas) até a condição no terceiro dia ou setenta e duas horas, os três sistemas não mostraram/se alterações estatisticamente significantes. A partir do tempo quarto dia ou 96 h., no sistema do grupo Cone Morse, revelou diferenças estatisticamente significantes entre o grupo HE e HI. Os resultados foram tabulados e o teste estatístico Anova há dois critérios e aplicados a eles o teste Tukey comparação entre todos, com o nível de significância de p<0.05. Os resultados do teste de vinte e quatro (24); quarenta e oito (48), setenta e duas (72), não havendo diferenças estatisticamente significantes, ao passo que no período de noventa e seis (96) e cento e quarenta e quatro(144) horas, mostrou a solução do corante de…
Advisors/Committee Members: Bonachela, Wellington Cardoso.
Subjects/Keywords: Espectrofotometria e solução azul de Evan; Extravasamento; Extravasation; Implant-abutment; Implante-abutment; Spectrophotometry and Evans blue solution
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APA ·
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CSE |
Export
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Manager
APA (6th Edition):
Auler e Salles, M. (2011). Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/25/25146/tde-14122011-091248/ ;
Chicago Manual of Style (16th Edition):
Auler e Salles, Murilo. “Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM).” 2011. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/25/25146/tde-14122011-091248/ ;.
MLA Handbook (7th Edition):
Auler e Salles, Murilo. “Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM).” 2011. Web. 19 Jan 2021.
Vancouver:
Auler e Salles M. Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM). [Internet] [Doctoral dissertation]. University of São Paulo; 2011. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-14122011-091248/ ;.
Council of Science Editors:
Auler e Salles M. Análise quantitativa do corante azul de Evan extravasado do interior das câmaras internas dos implantes por suas interfaces protéticas nas condições: Hexágono Externo (HE) - Hexágono Interno (HI) - Cone Morse (CM). [Doctoral Dissertation]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/25/25146/tde-14122011-091248/ ;
Penn State University
13.
Peng, Hsin-Hsin.
INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
.
Degree: 2008, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/7632
► The goal of this work is to identify key signaling pathways for melanoma-associated host cell responses during metastasis. Tumor cells have been shown to exploit…
(more)
▼ The goal of this work is to identify key signaling pathways for melanoma-associated host cell responses during metastasis. Tumor cells have been shown to exploit leukocyte function to enhance their metastatic properties under certain circumstances. Interleukin-8 (IL-8) is a chemotactic cytokine that regulates polymorphonuclear neutrophil (PMN) mobilization and activity, and we hypothesize this cytokine influences tumor behavior. We have demonstrated that IL-8 is crucial for PMN-mediated melanoma
extravasation under flow conditions. In addition, IL-8 is up-regulated in PMNs upon co-culturing with melanoma cells. Melanoma cells induce IƒÛB-ƒÑ degradation in PMNs indicating that nuclear factor ƒÛB (NF-ƒÛB) signaling is active in PMNs. Furthermore, the production of IL-8 in PMNs is NF-ƒÛB dependent. We have further identified that IL-6 and IL-1ƒÒ from PMN-melanoma co-cultures synergistically contribute to IƒÛB-ƒÑ degradation and IL-8 synthesis in PMNs.
Attachment of tumor cells to the endothelium has been shown to be critical for tumor metastasis. We have shown a phospholipase C (PLC)-mediated mechanism for the redistribution of interendothelial adherens junctions in response to melanoma cell contacts with the endothelium. We demonstrate that contact of melanoma cells to human umbilical vein endothelial cells trigger rapid endothelial [Ca2+]i response through PLC-IP3 pathway. In addition, alternation of endothelial adherens junctions following contact of melanoma cells have been evidenced by the changes in immunological staining patterns of vascular endothelial (VE)-cadherin. A PLC inhibitor, U73122 is shown to significantly diminish [Ca2+]i response and reduce the occurrence of melanoma cell¡Vinduced VE-cadherin reorganization. Moreover, inhibition of PLC attenuates melanoma cell transendothelial migration. However, melanoma cell-associated VE-cadherin breakdown is not sensitive to Ly294002, an inhibitor of phosphatidylinositol- 3-kinase (PI3K), whereas inhibition of PI3K resulted in a reduction of melanoma cell transmigration.
Taken together, we have shown that melanoma cells induce PMNs to secrete IL-8 through activation of NF-ƒÛB and suggest a model in which this interaction promotes a microenvironment that is favorable for metastasis. Moreover, by inducing the PLC-Ca2+ signaling pathway, melanoma cells disrupt endothelial junctions to breach the endothelium and promote transvascular homing of tumor cells. In addition to experimental approaches, a mathematic model has been established for studying the intracellular dynamics, such as Ca2+ signaling. The tool is beneficial for signaling pathway analysis and discovery.
Advisors/Committee Members: Cheng Dong, Committee Chair/Co-Chair, Andrew Thomas Henderson, Committee Member, Peter J Butler, Committee Member, Avery August, Committee Member.
Subjects/Keywords: cancer; signaling; adherens junction; chemokine; extravasation; cytokine
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APA ·
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CSE |
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APA (6th Edition):
Peng, H. (2008). INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/7632
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Peng, Hsin-Hsin. “INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
.” 2008. Thesis, Penn State University. Accessed January 19, 2021.
https://submit-etda.libraries.psu.edu/catalog/7632.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Peng, Hsin-Hsin. “INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
.” 2008. Web. 19 Jan 2021.
Vancouver:
Peng H. INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
. [Internet] [Thesis]. Penn State University; 2008. [cited 2021 Jan 19].
Available from: https://submit-etda.libraries.psu.edu/catalog/7632.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Peng H. INTERCELLULAR AND INTRACELLULAR SIGNALING DURING LEUKOCYTE-MEDIATED MELANOMA CELL TRANSENDOTHELIAL MIGRATION
. [Thesis]. Penn State University; 2008. Available from: https://submit-etda.libraries.psu.edu/catalog/7632
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Uppsala University
14.
Karim, Lara.
Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården.
Degree: Pharmaceutical Biosciences, 2020, Uppsala University
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415750
► Extravasation is a condition that can occur during an intravenous administration. This means that the solution administered intravenously goes extravascular. Depending on physiochemical properties…
(more)
▼ Extravasation is a condition that can occur during an intravenous administration. This means that the solution administered intravenously goes extravascular. Depending on physiochemical properties of the substance and solution, this can cause different severity of the damage. Extravasation of acyclovir, potassium phosphate and potassium chloride causes severe tissue damage that can, in worst case, lead to tissue necrosis. The purpose was therefore to investigate how acyclovir, potassium phosphate and potassium chloride causes tissue damage due to pH and osmolality and how the tissue damage can be avoided. To answer the purpose, a comprehensive literature search was conducted on three different databases; Pubmed, CINAHL and Cochrane. The literature search was in progress from February 4 to May 14 2020. The literature search generated a total of 42 articles and case reports, of which 13 of these were relevant for the purpose. These 13 articles consisted of two animal studies, three experimental observational studies, two guidelines from Västra Götalands Region, three case reports about acyclovir and three case reports of potassium phosphate and potassium chloride. Two of the experimental observational studies showed that potassium chloride could be diluted with 100 mL of 0,9% sodium chloride or 5% dextrose in water to possibly avoid tissue damage due to extravasation. Such results were not found for either acyclovir or potassium phosphate. The conclusion that could be drawn was that acyclovir caused tissue damage because of its alkaline pH, potassium phosphate because of its hyperosmolality and potassium chloride because of its acidic pH and its hyperosmolality. One way to possibly avoid tissue damage caused by extravasation is to dilute the substances with higher dilution volumes. However, due to the lack of reliability of the included studies, it cannot be safely concluded that tissue damage can be avoided.
Subjects/Keywords: Extravasation; acyclovir; potassium phosphate; potassium chloride; pH; osmolality; tissue damage; Social and Clinical Pharmacy; Samhällsfarmaci och klinisk farmaci
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APA (6th Edition):
Karim, L. (2020). Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården. (Thesis). Uppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Karim, Lara. “Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården.” 2020. Thesis, Uppsala University. Accessed January 19, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Karim, Lara. “Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården.” 2020. Web. 19 Jan 2021.
Vancouver:
Karim L. Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården. [Internet] [Thesis]. Uppsala University; 2020. [cited 2021 Jan 19].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415750.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Karim L. Extravasering vid behandlingar med aciklovir, kaliumfosfat och kaliumklorid inom intensivvården. [Thesis]. Uppsala University; 2020. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-415750
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
15.
Medina Rodriguez, Indira A.
Modulation of leukocyte homeostasis in atherosclerosis.
Degree: 2014, Department of Biopharmaceutics, Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University
URL: http://hdl.handle.net/1887/25765
► Many of the recognition molecules and mechanisms involved in immune responses have no bias towards external stimuli, but also sense and respond to pathological and…
(more)
▼ Many of the recognition molecules and mechanisms involved in immune responses have no bias towards external stimuli, but also sense and respond to pathological and physiological changes of non infectious origin taking place within the body.
Aiming at defining potential immuno-therapeutic strategies to treat human atherosclerosis, the focus of this work was the modulation of immune processes determinant of atherosclerosis lesion progression or cessation in mice, such as hematopoiesis, diapedesis and intravasation, leukocyte differentiation, cholesterol uptake apoptosis and cell survival.
Modulation of these processes, by using bone marrow transplantation of hematopoietic stem cells with genetic deficiencies or over-expressing human or mouse engineered genes, demonstrated to alter the fate of atherosclerotic lesions at the balance between macrophage accumulation and lesion vulnerability versus resolution of inflammation and wound healing.
This thesis demonstrates that processes responsible for the development and progression of atherosclerosis are dynamic and can be modulated to induce lesion stabilization and disease resolution. These results are promising for the development of novel therapeutics and challenge the current notion that atherosclerosis has a predetermined fate towards lesion vulnerability to rupture, which in humans results in thrombosis and clinical manifestations such myocardial infarction or stroke and sudden death.
Subjects/Keywords: Atherosclerosis; Apoptosis; Migration; Diapedesis; Differentiation; Extravasation; Intravasation; Hematopoieisis; Macrophage; Chemotaxis; Atherosclerosis; Apoptosis; Migration; Diapedesis; Differentiation; Extravasation; Intravasation; Hematopoieisis; Macrophage; Chemotaxis
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APA (6th Edition):
Medina Rodriguez, I. A. (2014). Modulation of leukocyte homeostasis in atherosclerosis. (Doctoral Dissertation). Department of Biopharmaceutics, Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University. Retrieved from http://hdl.handle.net/1887/25765
Chicago Manual of Style (16th Edition):
Medina Rodriguez, Indira A. “Modulation of leukocyte homeostasis in atherosclerosis.” 2014. Doctoral Dissertation, Department of Biopharmaceutics, Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University. Accessed January 19, 2021.
http://hdl.handle.net/1887/25765.
MLA Handbook (7th Edition):
Medina Rodriguez, Indira A. “Modulation of leukocyte homeostasis in atherosclerosis.” 2014. Web. 19 Jan 2021.
Vancouver:
Medina Rodriguez IA. Modulation of leukocyte homeostasis in atherosclerosis. [Internet] [Doctoral dissertation]. Department of Biopharmaceutics, Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University; 2014. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1887/25765.
Council of Science Editors:
Medina Rodriguez IA. Modulation of leukocyte homeostasis in atherosclerosis. [Doctoral Dissertation]. Department of Biopharmaceutics, Leiden Academic Center for Drug Research (LACDR), Faculty of Science, Leiden University; 2014. Available from: http://hdl.handle.net/1887/25765
Freie Universität Berlin
16.
Feller, Jewgenij.
Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia.
Degree: 2013, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-13094
► Despite significant advances in intensive care and research, the sepsis due to steadily increasing incidence and high mortality unchanged becoming an increasingly important problem in…
(more)
▼ Despite significant advances in intensive care and research, the sepsis due to
steadily increasing incidence and high mortality unchanged becoming an
increasingly important problem in the ICU. A generalized inflammatory response
is the focus of sepsis. The pathophysiological final pathway involves
excessive activation of humoral and cellular cascade systems. The consequences
are disturbances in microcirculation, increased leukocyte-endothelium-
interaction and damage of endothelial integrity with subsequent edema and
organ dysfunction. Furthermore, there is an activation of the complement
system, contact phase system and the coagulation. The excessive activation of
plasmatic cascade systems leads to a consumption of the factors. A potential
therapeutic option for the treatment of sepsis is to influence these cascades
with medicaments. C1 inhibitor is the main inhibitor of the classical pathway
of complement activation, contact phase system and the coagulation.
Coagulation factor XIII has beside his coagulation-active effect still other
functions, such as the influencing of the cell adhesion / cell migration and
stabilisation of the endothelial barrier. The disturbance of the
microcirculation of the intestine plays a central role in the pathogenesis of
sepsis and multiple organ failure. Therefore, the influence of the drug
combination of C1-esterase inhibitor and coagulation factor XIII was examined
in the mesenteric microcirculation of the present study in an established
model of sepsis in rats by intravital microscopy. The leucocyte-endothelial
interactions served as the measure of the leucocyte activation. The plasma
extravasation was recognized as a parameter for the damage to the endothelial
integrity quantitatively. In this study, we have shown that the drug
combination of C1-esterase inhibitor, and coagulation factor XIII causes a
significant reduction of firmly adherent leukocytes in mesenteric venules
against the endotoxin group. However, we could show no influence of the drug
combination at the temporarily adherent leukocytes or plasma
extravasation.
The administration of the drug combination C1 esterase inhibitor with
coagulation factor XIII has significantly reduced the endotoxin-induced
increase in the plasmatic concentration of IL-1β. However, there was an
increased secretion of IL-6 in the C1-INH + F XIII group, which could indicate
a possible proinflammatory effect of the drug combination. Further studies are
required to investigate the effects of the combination of C1-esterase
inhibitor, and coagulation factor XIII during sepsis, for example in different
dosages.
Advisors/Committee Members: [email protected] (contact), m (gender), Priv.-Doz. Dr.med. J. Birnbaum (firstReferee), Priv.-Doz. Dr.med. H. Kern (furtherReferee), Priv.-Doz. Dr.med. M. Hensel (furtherReferee).
Subjects/Keywords: sepsis; intravital microscopy; plasma extravasation; leukocyte activation; leukocyte-endothelium interaction; C1 esterase inhibi; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Feller, J. (2013). Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-13094
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Feller, Jewgenij. “Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia.” 2013. Thesis, Freie Universität Berlin. Accessed January 19, 2021.
http://dx.doi.org/10.17169/refubium-13094.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Feller, Jewgenij. “Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia.” 2013. Web. 19 Jan 2021.
Vancouver:
Feller J. Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Jan 19].
Available from: http://dx.doi.org/10.17169/refubium-13094.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Feller J. Influence of C1 esterase inhibitor in combination with coagulation factor XIII
to the mesenteric microcirculation in experimental endotoxaemia. [Thesis]. Freie Universität Berlin; 2013. Available from: http://dx.doi.org/10.17169/refubium-13094
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Western Ontario
17.
Kim, Yohan.
Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model.
Degree: 2019, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/6745
► Cancer metastasis is a multistep process that begins with the invasion of tumour cells into the stroma and migration towards the blood vessels. Tumour cells…
(more)
▼ Cancer metastasis is a multistep process that begins with the invasion of tumour cells into the stroma and migration towards the blood vessels. Tumour cells that have entered the bloodstream must then survive and leave by a process known as extravasation. Finally, extravasated cells proliferate and establish the secondary site in the metastatic cascade. Although extravasation encompasses key events during cancer cell invasion to aid in the development of effective treatments, an in vivo model that rapidly, reproducibly and economically recapitulates cancer cell extravasation is needed. Therefore, the objectives of my research were to 1) establish and validate an in vivo model of cancer cell extravasation, and 2) identify novel cellular and molecular events.
I used the chorioallantoic membrane of chicken embryos as a model system of extravasation as it provides an accessible and highly vascularized structure. The combination of the chorioallantoic membrane of chicken embryos, nanoscale flow cytometry, and confocal microscopy-based intravital imaging allowed me to observe that extravasating prostate cancer cells exhibited significant cell volume reduction. This reduction is suggestive of an invasive cell phenotype. However, cell volume reduction at certain threshold also decreased cancer cell extravasation efficiency. I also found that cancer cell released extravascular vesicles during extravasation, and an increase in extracellular vesicle release reduced cell volume. I then tested the hypothesis that extracellular vesicle release and extravasation may be linked to modes of cell death. Real-time imaging of extravasating cancer cells that released extracellular vesicles did not show activation of caspase-3. Activation of necroptosis, however, increased extracellular vesicle release and decreased cell extravasation and secondary colony formation. These results suggest that necroptosis may be targeted to induce extracellular vesicle release, decrease extravasation, and halt cancer metastasis.
Collectively, my work lays out the protocols for the use of the chorioallantoic membrane of chicken embryos as a model system to investigate cancer cell extravasation and invasion. Use of this model system allowed me to identify extracellular vesicle release during extravasation and discover that necroptosis may be a potential regulator of cancer metastasis.
Subjects/Keywords: Cancer metastasis; chorioallantoic membrane; metastatic cascade; extravasation; necroptosis; MLKL; Biological Phenomena, Cell Phenomena, and Immunity; Disease Modeling; Medical Biochemistry; Medical Cell Biology; Medical Pathology; Molecular Biology; Neoplasms
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kim, Y. (2019). Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6745
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kim, Yohan. “Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model.” 2019. Thesis, University of Western Ontario. Accessed January 19, 2021.
https://ir.lib.uwo.ca/etd/6745.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kim, Yohan. “Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model.” 2019. Web. 19 Jan 2021.
Vancouver:
Kim Y. Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2021 Jan 19].
Available from: https://ir.lib.uwo.ca/etd/6745.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kim Y. Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6745
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of South Florida
18.
Opp, Daniel.
Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate.
Degree: 2007, University of South Florida
URL: https://scholarcommons.usf.edu/etd/3808
► Cigarette smoke's influence on cancer has primarily been a subject of epidemilogic and tumorigenic studies. There have been no proper investigations with interests focused on…
(more)
▼ Cigarette smoke's influence on cancer has primarily been a subject of epidemilogic and tumorigenic studies. There have been no proper investigations with interests focused on how cigarette smoke affects the cellular mechanics of metastasis. Gathering an understanding of how smoke influences metastatic invasion could be vital in regulating or possibly eliminatings cancer's ability to initiate new tumor growth sites. This project focuses on cigarette smoke's influence on cellular mechanics of endothelial cells, and the invasive potential of cancer against a fully active endothelium. It is already known that cigarette smoke has a carcinogenic effect, but it is hypothesized that the cigarette smoke causes the endothelium to exhibit pro-invasive characteristics. Cancer cells are often ignorant to extra-cellular stimuli. It is suspected that there will be a less pronounced degradation of cellular mechanics of cancerous cells than endothelial cells when exposed to similar concentrations of cigarette smoke.
Subjects/Keywords: ECIS; junctional resistance; cell adhesion molecules; intravasation; extravasation; American Studies; Arts and Humanities
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Opp, D. (2007). Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/3808
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Opp, Daniel. “Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate.” 2007. Thesis, University of South Florida. Accessed January 19, 2021.
https://scholarcommons.usf.edu/etd/3808.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Opp, Daniel. “Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate.” 2007. Web. 19 Jan 2021.
Vancouver:
Opp D. Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate. [Internet] [Thesis]. University of South Florida; 2007. [cited 2021 Jan 19].
Available from: https://scholarcommons.usf.edu/etd/3808.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Opp D. Transendothelial Migration of Metastatic Cancer Under the Influence of Cigarette Smoke Condensate. [Thesis]. University of South Florida; 2007. Available from: https://scholarcommons.usf.edu/etd/3808
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
19.
Wenger, Andrew.
Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs.
Degree: 2019, University of Waterloo
URL: http://hdl.handle.net/10012/14719
► Colorectal cancer is the 2nd most common form of cancer in Canada. Most colorectal cancer deaths are caused by complications that stem from the metastasis…
(more)
▼ Colorectal cancer is the 2nd most common form of cancer in Canada. Most colorectal cancer
deaths are caused by complications that stem from the metastasis of colorectal cancer to other
organs such as the liver. During metastasis, the extravasation step of the metastasis cascade
involves the attachment of circulating tumour cells to the endothelial cell layer that coats the inside
of human blood vessels and the eventual migration of the cells past this layer. Current tumour
extravasation models, used for drug and mechanistic studies, do not accurately reflect the microenvironments found in the human body. Three dimensional bioprinting has become an attractive
method to produce extravasation models. This thesis explores the development of a hybrid
hydrogel bio-ink consisting of alginate, GelMA and cellulose nanocrystals to create cell scaffolds
that can support the growth of a monolayer of endothelial cells. A bio-ink consisting of 2 wt%
alginate, 4 wt% GelMA and 6 wt% cellulose nanocrystals was chosen for printability studies and
cellular studies for its high shear thinning and low hysteresis in viscosity recovery. Scaffolds
designed to mimic a human blood vessel and villi structures in a healthy colon were printed and
EA-hy 926 cells were seeded on top. After six days and an additional seeding of cells it was found
that the bio-ink could not sustain the growth of a complete mono-layer of EA-hy 926 cells. The
high electrostatic repulsion between the negatively charged cell membrane and the negatively
charged alginate and cellulose nanocrystals is thought to play a role in the ability of the cells to
attach to the scaffold surface. Scaffold treated with poly(L-lysine) were made and EA-hy-926 cells
were again seeded on top. This resulted in better cell distribution, but increased instances of
cellular elongation suggested a decrease in cellular adhesion. In both poly(L-lysine) treated and
un-treated scaffolds the cells were able to attach and proliferate on top of the scaffolds suggesting
that with some changes in either the bio-ink’s formula, cell seeding densities or post printing
treatments a mono layer of cells could be formed to be used in the production of extravasation
models of colorectal cancer.
Subjects/Keywords: 3D bioprinting; Hydrogel; bio-ink; colorectal cancer; metastasis; extravasation
…through
extravasation in which they enter the tissue surrounding the blood vessel. 6,7
1.1.2… …proliferation of the tumour cells.11
The extravasation of cancer cells from the vasculature structures… …metastases cascade. Transwell
3
assay have commonly been used to study extravasation and have… …determined many molecular
mechanisms and components crucial to the extravasation process. This… …extravasation process. (b)
Schematic of in-vivo extravasation assay using chorioallantoic…
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wenger, A. (2019). Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/14719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wenger, Andrew. “Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs.” 2019. Thesis, University of Waterloo. Accessed January 19, 2021.
http://hdl.handle.net/10012/14719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wenger, Andrew. “Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs.” 2019. Web. 19 Jan 2021.
Vancouver:
Wenger A. Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs. [Internet] [Thesis]. University of Waterloo; 2019. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10012/14719.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wenger A. Development of a Hybrid Hydrogel Bio-ink for 3D Printing of Biomimetic Tissue Constructs. [Thesis]. University of Waterloo; 2019. Available from: http://hdl.handle.net/10012/14719
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toledo Health Science Campus
20.
Goswamee, Priyodarshan.
The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model.
Degree: PhD, Biomedical Sciences (Neurosciences and Neurological
Disorders), 2015, University of Toledo Health Science Campus
URL: http://rave.ohiolink.edu/etdc/view?acc_num=mco1438280470
► Gastroenteropancreatic neuroendocrine tumors (GEPNETs) of the midgut are a heterogeneous group of cancers that typically remain asymptomatic until they metastasize to the liver. However, the…
(more)
▼ Gastroenteropancreatic neuroendocrine tumors (GEPNETs)
of the midgut are a heterogeneous group of cancers that typically
remain asymptomatic until they metastasize to the liver. However,
the mechanism by which these usually indolent neoplasms establish
distal metastasis remains unclear. The results presented in this
dissertation support the hypothesis that arachidonic acid-induced
Ca
2+ entry through an Orai3-containing
channel enhances GEPNET cell migration and contribute to tumor cell
extravasation through the hepatic microvasculature. Using a novel
liver slice preparation in combination with ultrastructure
analysis, we demonstrated that a subpopulation of BON cells, a
well-characterized GEPNET cell line, opportunistically migrated
through the discontinuous sinusoidal endothelium and redistributed
into the liver parenchyma within 24 hours following their
introduction to the sinusoids. In a parallel set of studies, we
identified a novel Ca
2+ entry pathway
through an Orai-containing channel that enhanced the migratory
capacity of a subpopulation of these tumor cells. Orai-containing
channels are known to underlie Ca
2+ entry in
response to both store-depletion and activation by arachidonic acid
(AA) or its metabolites. In recent years, the store-operated
Ca
2+ entry (SOCE) has been implicated in the
migration of some cancer cell lines. However, the role of
AA-induced Ca
2+ entry in cancer cell
migration has not been adequately assessed. We investigated the
involvement of AA-induced Ca
2+ entry in
migration in BON cells using live cell fluorescence imaging and
standard migration assays in combination with pharmacological and
gene knockdown methods. We showed that both SOCE and AA-induced
Ca
2+ entry modes could be selectively
activated and inhibited. Administration of AA resulted in
Ca
2+ entry that was pharmacologically
distinct from SOCE. Moreover, whereas homomeric Orai1-containing
channels appeared to largely underlie SOCE, the AA-activated
Ca
2+ channel required expression of Orai3 as
well as, Orai1. Furthermore, we showed that treatment with AA
enhanced migration of BON cells and that this enhancement could be
abrogated by selective inhibition of the AA-induced
Ca
2+ entry, whereas activation of SOCE did
not enhance migration. In addition, the AA-induced
Ca
2+ entry and SOCE appeared to be
reciprocally repressive suggesting the possibility that the balance
between these two pathways sets the migratory potential of these
tumor cells.Taken together, we identified
Ca
2+ entry through the Orai3-containing
channel as a novel signal for BON cell migration that may be
exploited to develop therapies to limit recurring GEPNET
metastasis.
Advisors/Committee Members: Giovannucci, David (Committee Chair).
Subjects/Keywords: Biomedical Research; Calcium signaling; Orai1; Orai3; Arachidonate induced Calcium entry; Store operated Calcium entry; Tumor extravasation; Liver metastasis; Carcinoids; Gastroenteropancreatic neuroendocrine tumors
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APA ·
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Manager
APA (6th Edition):
Goswamee, P. (2015). The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model. (Doctoral Dissertation). University of Toledo Health Science Campus. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=mco1438280470
Chicago Manual of Style (16th Edition):
Goswamee, Priyodarshan. “The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model.” 2015. Doctoral Dissertation, University of Toledo Health Science Campus. Accessed January 19, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=mco1438280470.
MLA Handbook (7th Edition):
Goswamee, Priyodarshan. “The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model.” 2015. Web. 19 Jan 2021.
Vancouver:
Goswamee P. The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model. [Internet] [Doctoral dissertation]. University of Toledo Health Science Campus; 2015. [cited 2021 Jan 19].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1438280470.
Council of Science Editors:
Goswamee P. The Role of Orai-Mediated Ca2+ Entry
in Migration in a Gastroenteropancreatic Neuroendocrine Tumor
Model. [Doctoral Dissertation]. University of Toledo Health Science Campus; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=mco1438280470
21.
Winkler, Clayton Wade.
Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44.
Degree: PhD, 2012, Oregon Health Sciences University
URL: doi:10.6083/M4BV7DNP
;
http://digitalcommons.ohsu.edu/etd/895
Subjects/Keywords: Multiple sclerosis; Blood-brain barrier; Endothelial cells; Lymphocytes; Extravasation; Multiple Sclerosis; Blood-Brain Barrier; Endothelial Cells; Lymphocytes
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APA (6th Edition):
Winkler, C. W. (2012). Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4BV7DNP ; http://digitalcommons.ohsu.edu/etd/895
Chicago Manual of Style (16th Edition):
Winkler, Clayton Wade. “Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44.” 2012. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021.
doi:10.6083/M4BV7DNP ; http://digitalcommons.ohsu.edu/etd/895.
MLA Handbook (7th Edition):
Winkler, Clayton Wade. “Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44.” 2012. Web. 19 Jan 2021.
Vancouver:
Winkler CW. Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 2012. [cited 2021 Jan 19].
Available from: doi:10.6083/M4BV7DNP ; http://digitalcommons.ohsu.edu/etd/895.
Council of Science Editors:
Winkler CW. Hyaluronan and its degradation products have opposing effects on experimental autoimmune encephalomyelitis pathogenesis that are dependent and independent of CD44. [Doctoral Dissertation]. Oregon Health Sciences University; 2012. Available from: doi:10.6083/M4BV7DNP ; http://digitalcommons.ohsu.edu/etd/895
Freie Universität Berlin
22.
Kilian, Karin.
Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin.
Degree: 2002, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-11401
► Ruhende Leukozyten zirkulieren mit dem Blutstrom durch den Koerper, m¸ssen die Gefaesse jedoch verlassen, um ihre immunologische Funktion auszuueben. Die gezielte Auswanderung aus den Gefaessen…
(more)
▼ Ruhende Leukozyten zirkulieren mit dem Blutstrom durch den Koerper, m¸ssen die
Gefaesse jedoch verlassen, um ihre immunologische Funktion auszuueben. Die
gezielte Auswanderung aus den Gefaessen waehrend Entzuendungsreaktionen und
Lymphocyten-Rezirkulation wird durch das Zusammenspiel verschiedener
leukozytaerer und endothelialer Adhaesionsmolekuele sowie proinflammatorischer
Aktivatoren reguliert, die eine mehrstufige Adhaesionskaskase bilden.
L-Selektin, ein Kohlehydrat-bindendes Adhaesionsmolekuel auf Leukozyten,
vermittelt den ersten Schritt dieser Kaskade durch transienter Kontakt mit
vaskulaeren Mucin-aehnlichen Adhaesionsmolekuelen. Diese Interaktion fuehrt
zum langsamen Rollen der Leukozyten entlang der Gefaesswand, das die
Vorraussetzung fuer die feste Anheftung der Blutzellen an das Endothel ist.
Neben der Rolle bei der initialen Adhaesion aktiviert L-Selektin
intrazellulaere Signalwege in Leukozyten und wird selbst durch Signale aus dem
Inneren der Zelle in seiner Bindungsaktivitaet moduliert. Verstaerkte Bindung
kann nach Chemokin-Behandlung oder zellulaere Aktivierung durch den T-Zell-
Rezeptor beobachtet werden. Es wird vermutet, dass diese Aktivierung durch
eine Phosphorylierung der zytoplasmatischen Domaene des Rezeptors an Serin-
Resten verursacht wird. Obwohl zahlreiche zellulaere Signalwege bekannt sind,
die von L-Selektin beeinflusst werden, sind die Vorgaenge auf der Ebene des
Rezeptors unbekannt. Das Ziel dieser Arbeit war daher die Isolation
intrazellulaerer Interaktionspartner von L-Selektin, die eine Rolle in der
Signaluebermittlung spielen. Des weiteren sollten Kinasen identifiziert
werden, die die Phosphorylieung des Rezeptors vermitteln. Zu diesem Zweck
wurde die Faehigkeit verschiedener Serin/Threonin Kinasen zur Phosphorylierung
der cytoplasmatischen Domaene von L-Selektin getestet. Sowohl Protein Kinase C
(PKC) als cGMP-abhaengige Protein Kinase (PKG) sind in der Lage, diese Sequenz
als Substrat zu verwenden, waehrend Protein Kinase A keine Aktivitaet
gegenueber diesem Protein besitzt. Durch Affinitaetsreinigung wurde eine
Kinase-Aktivitaet aus Zellysaten isoliert, die fest an die zytoplasmatische
Domaene von L-Selektin bindet und diese an Serin-Resten phosphoryliert.
Inhibitorstudien zeigten, dass diese Kinase durch spezifische PKC-Antagonisten
hemmbar ist. Durch Immunodetektion konnten zwei PKC-Isozyme nachgewiesen
werden, die mit einem Lscyto Fusionsprotein assozierten: neuartige PKCq und
atypische PKCl. Die Suche nach Interaktionspartnern, die spezifisch an die
Serin-phosphorylierte Form von L-Selektin binden, fuehrte zu Isolation zweier
Proteine, die als konventionelle PKCa und wiederum PKCq identifiziert wurden.
PKCq bindet phosphorylieres L-Selektin in staerkerem Ausmass als das nicht-
modifizierte Protein. Sowohl PKCa als auch PKCq co-immunpraezipitieren mit
L-Selektin aus PMA-behandelten T-Zell-Lysaten, was zeigt, dass diese
Interaktion in intakten Zellen stattfindet. Diese Untersuchung zeigt, dass
eine Isozym-spezifische Assoziation von PKC mit L-Selektin stattfindet und
dass…
Advisors/Committee Members: n (gender), Prof. Dr. Rudolf Tauber (firstReferee), Prof. Dr. Ferdinand Hucho (furtherReferee).
Subjects/Keywords: L-selectin; selectins; extravasation; signal transduction; PKC; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kilian, K. (2002). Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-11401
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kilian, Karin. “Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin.” 2002. Thesis, Freie Universität Berlin. Accessed January 19, 2021.
http://dx.doi.org/10.17169/refubium-11401.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kilian, Karin. “Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin.” 2002. Web. 19 Jan 2021.
Vancouver:
Kilian K. Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin. [Internet] [Thesis]. Freie Universität Berlin; 2002. [cited 2021 Jan 19].
Available from: http://dx.doi.org/10.17169/refubium-11401.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kilian K. Identifikation neuer intrazellulärer Interaktionspartner des leukozytären
Adhäsionsmoleküls L-Selektin. [Thesis]. Freie Universität Berlin; 2002. Available from: http://dx.doi.org/10.17169/refubium-11401
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Houston
23.
Magadi, Sri.
Corneal Wound Healing: Cells and Molecules.
Degree: PhD, Physiological Optics and Vision Science, University of Houston
URL: http://hdl.handle.net/10657/3522
► Purpose: Corneal abrasion elicits an acute inflammatory response involving neutrophil (PMN) and platelet extravasation from the limbus. While these cells deliver important growth factors that…
(more)
▼ Purpose: Corneal abrasion elicits an acute inflammatory response involving neutrophil (PMN) and platelet
extravasation from the limbus. While these cells deliver important growth factors that promote wound healing, resolution of the inflammatory response (i.e., PMN clearance) is also needed to restore corneal homeostasis. This dissertation evaluated mechanisms regulating platelet recruitment after corneal injury, PMN migration in the abraded mouse corneal stroma and PMN clearance during the resolution of inflammation.
Methods: Adult C57BL/6J mice were anesthetized and a 2mm central corneal abrasion was made using a golf-spud/Alger brush. As a means of dysregulating platelet
extravasation, approaches were used that build on the established observation that platelet recruitment is linked to PMN recruitment. Some mice received an intraperitoneal injection of anti-Ly6G antibody to deplete circulating PMNs while others received topical applications of recombinant interleukin-20 (rIL-20) to blunt inflammation. To determine if platelet
extravasation was dependent on CD18 expression levels, two groups of mutant mice were studied, one with intermediate-high CD18 expression (CD18hypo (I-H)) and one with low-intermediate CD18 expression (CD18hypo (L-I)). To evaluate inflammation, excised corneas were immunolabelled and PMNs, platelets, mast cells, and degranulated mast cells were counted and limbal vessel diameters were measured. To evaluate PMN surface contacts with other cells and the extracellular matrix, excised corneas were imaged using serial block-face scanning electron microscopy. To study PMN migration in vivo, time-lapse sequences were collected using the Heidelberg Retinal Tomographer III with Rostock Cornea module (HRT-RCM).
Results: Following a central corneal abrasion, IL-20-treated mice and anti-Ly6G treated mice showed reduced PMN and platelet
extravasation. PMN
extravasation in CD18hypo (I-H) mice was similar to WT mice while platelet
extravasation was diminished (~80%) only in CD18hypo (L-I) mice. Passive transfer of WT PMNs into CD18hypo (L-I) mice did not restore platelet
extravasation to WT levels. In all cases, mice with diminished platelet recruitment showed diminished limbal vessel dilatation and reduced mast cell degranulation (p≤ 0.05).
8h post-injury, majority (70%) of paralimbal PMNs close to the limbus moved circumferentially with an average speed of 3.1±0.4µm/min, significantly less than the 6.1±0.3µm/min speed of PMNs at the paralimbus/closer to the center of the cornea where majority (60%) of these PMNs showed oriented migration toward the wound (p≤0.05). Serial block-face data provided evidence for a circumferential orientation of corneal cells and collagen at the paralimbus. Here, PMN surfaces predominantly contacted collagen (~60%), and keratocytes (~21%). At the center of the cornea, PMN numbers were maximal at 24h and markedly reduced by 72h post-injury. 24h post-injury, 64% of central PMNs were migrating away from the center which was significantly higher than that observed…
Advisors/Committee Members: Burns, Alan R. (advisor), Redfern, Rachel (committee member), Hanlon, Samuel (committee member), Miller, William L. (committee member), Rumbaut, Rolando E. (committee member).
Subjects/Keywords: Corneal wound healing; Reverse migration; CD18; Platelet extravasation; IL-20; Ly6G; Mast cells; Neutrophils
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APA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Magadi, S. (n.d.). Corneal Wound Healing: Cells and Molecules. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/3522
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Magadi, Sri. “Corneal Wound Healing: Cells and Molecules.” Doctoral Dissertation, University of Houston. Accessed January 19, 2021.
http://hdl.handle.net/10657/3522.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Magadi, Sri. “Corneal Wound Healing: Cells and Molecules.” Web. 19 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Magadi S. Corneal Wound Healing: Cells and Molecules. [Internet] [Doctoral dissertation]. University of Houston; [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10657/3522.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Magadi S. Corneal Wound Healing: Cells and Molecules. [Doctoral Dissertation]. University of Houston; Available from: http://hdl.handle.net/10657/3522
Note: this citation may be lacking information needed for this citation format:
No year of publication.
24.
Leu, Kevin.
Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange.
Degree: Bioengineering, 2017, UCLA
URL: http://www.escholarship.org/uc/item/43z64715
► Magnetic resonance imaging (MRI) plays an integral role in the diagnosis and monitoring of gliomas. One means by which MRI has been used to assess…
(more)
▼ Magnetic resonance imaging (MRI) plays an integral role in the diagnosis and monitoring of gliomas. One means by which MRI has been used to assess treatment efficacy has been measuring the volumes of contrast-enhancing lesions on post-contrast T1-weighted images. Clinically, an increase of the lesion volume by a certain percentage compared to a previous baseline scan warrants a change in treatment. However, this type of imaging has its limitations, as exemplified by false positive radiographic determination of tumor progression (“pseudoprogression”) and false positive radiographic determination of treatment response (“pseudoresponse”). Given the vascular nature of gliomas, perfusion-weighted dynamic susceptibility contrast (DSC)-MRI has been studied in efforts to improve the detection, characterization, and monitoring of gliomas after treatment. However, applying DSC-MRI biomarkers is not necessarily straightforward. One of the biggest problems with the calculation of relative cerebral blood volume (rCBV) is that it is compromised by artifacts created by the extravasation of contrast agent from the vasculature. This can be a particular challenge in the neuro-oncology field since blood brain barrier disruption is a common feature of gliomas.This work attempts to improve estimates of rCBV in gliomas by incorporating a two-compartment pharmacokinetic model into the indicator dilution theory, which we term the “bidirectional” leakage correction. In Chapter II, we use simulation methods to demonstrate improved accuracy gained by the bidirectional leakage correction, as compared to a current, popular leakage correction (“unidirectional” leakage correction). In Chapter III, we demonstrate that the bidirectional model-generated permeability curves have better correlation with DCE-MRI permeability curves than those generated by the unidirectional model. We also demonstrate that the rCBV is more similar for the bidirectional model between two separate pre-treatment scans from the same patient. In Chapter IV, we demonstrate that the change in bidirectional rCBV can stratify glioblastoma patients treated with bevacizumab according to long- or short-term survival. In all, the above works demonstrate that the new technique better combats leakage effects, thereby improving the clinical utility of rCBV for human gliomas.
Subjects/Keywords: Biomedical engineering; Biophysics; Contrast Agent Extravasation; DSC-MRI; Glioma; Leakage Correction; MRI; Perfusion
…6
iv. Modeling DSC-MRI in the Presence of Contrast Agent Extravasation… …Extravasation
However, an extra complication exists in gliomas in that the gadolinium-based
contrast… …gadolinium extravasation, we start with the gradient echo
signal equation:
!"
!"… …1.4. Example of the effects of the contrast agent extravasation on the DSC-MRI
curve. The… …that allows for the computation of contrast agent extravasation into the
tissue. The latter…
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Leu, K. (2017). Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/43z64715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Leu, Kevin. “Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange.” 2017. Thesis, UCLA. Accessed January 19, 2021.
http://www.escholarship.org/uc/item/43z64715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Leu, Kevin. “Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange.” 2017. Web. 19 Jan 2021.
Vancouver:
Leu K. Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange. [Internet] [Thesis]. UCLA; 2017. [cited 2021 Jan 19].
Available from: http://www.escholarship.org/uc/item/43z64715.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Leu K. Improved Accuracy of Dynamic Susceptibility Contrast Magnetic Resonance Imaging Estimates of Relative Cerebral Blood Volume in Human Gliomas by Accounting for Bidirectional Contrast Agent Exchange. [Thesis]. UCLA; 2017. Available from: http://www.escholarship.org/uc/item/43z64715
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of South Florida
25.
Opp, Daniel.
ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells.
Degree: 2009, University of South Florida
URL: https://scholarcommons.usf.edu/etd/2125
► The investigations conducted within this dissertation centers around the use of electric cell-substrate impedance sensing (ECIS). This system is able to characterize in real-time analysis,…
(more)
▼ The investigations conducted within this dissertation centers around the use of electric cell-substrate impedance sensing (ECIS). This system is able to characterize in real-time analysis, the adhesion of cells to their substrate and neighboring cells. With this, valuable information can be gathered with in-vitro experiments regarding a tissue culture's response to physiological stimulation. This dissertation has taken advantage of ECIS' ability to analyze toxicology, barrier function, and cancer invasion on a tissue culture. With proper analysis modifications, trans-epethelial resistance (TER) can be used as a cytotoxicity assay with higher sensitivity than previously thought. In vitro assessment of cytotoxicity based on TER needs more quantitative methods to analyze the alteration of cell morphology and motility.
Here, we applied ECIS to evaluate dose-dependent responses of human umbilical vein endothelial cells (HUVEC) and mouse embryonic fibroblasts (NIH 3T3) exposed to cytochalasin B and protein kinase inhibitor H7. To detect subtle changes in cell morphology, the frequency-dependent impedance data of the cell monolayer were measured and analyzed with a theoretical cell-electrode model. To detect the alternation of cell micromotion in response to cytochalasin B and H7 challenge, time-series impedance fluctuations of cell-covered electrodes were monitored and the values of power spectrum, variance, and variance of the increment were calculated to verify the difference. While a dose-dependent relationship was generally observed from the overall resistance of the cell monolayer, the analysis of frequency-dependent impedance and impedance fluctuations distinguished cytochalasin B levels as low as 0.1µM and H7 levels as low as 10 µM for HUVEC and 3T3 layers.
Even though overall resistance values are relatively small for 3T3 layers, and frequency scan measurements are negligible, impedance fluctuation analysis reveals significant micromotion for cytotoxic detection. Our results show that cytochalasin B and H7 causes a decrease of junctional resistance between cells and an increase of membrane capacitance. Cigarette smoke is cytotoxic and tumorigenic. Initial studies were conducted to evaluate the cytotoxicity of cigarette smoke condensate (CSC) on HUVEC layers. The focus was then turned to investigations involving in vitro cancer invasion assays with CSC on HUVEC layers. ECIS is an excellent investigative device that can be utilized to observe cancer invasion on normal tissue cultures due to the significantly higher impedance signature of cancer cells.
The investigation in this dissertation focused on cigarette smoke's influence on cellular mechanics of endothelial cells and the invasive potential of two ovarian cancer cell lines (ALST and OVCA429) against a fully active endothelium. The HUVEC cultures responded to CSC with an increase in junctional binding, where as ALST and OVCA429 relieved adhesion thereby providing an improved motility when evaluated in wound healing assays. Transmigration of the HUVEC layer by…
Subjects/Keywords: Micromotion; Cytochalasin B; Protien kinase inhibitor H7; Intravasation; Extravasation; American Studies; Arts and Humanities
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Opp, D. (2009). ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells. (Thesis). University of South Florida. Retrieved from https://scholarcommons.usf.edu/etd/2125
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Opp, Daniel. “ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells.” 2009. Thesis, University of South Florida. Accessed January 19, 2021.
https://scholarcommons.usf.edu/etd/2125.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Opp, Daniel. “ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells.” 2009. Web. 19 Jan 2021.
Vancouver:
Opp D. ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells. [Internet] [Thesis]. University of South Florida; 2009. [cited 2021 Jan 19].
Available from: https://scholarcommons.usf.edu/etd/2125.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Opp D. ECIS assessment of cytotoxicity and trans-endothelial migration of metastatic cancer cells. [Thesis]. University of South Florida; 2009. Available from: https://scholarcommons.usf.edu/etd/2125
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Georgia Tech
26.
Jambulingam, Jambu.
Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy.
Degree: MS, Electrical and Computer Engineering, 2015, Georgia Tech
URL: http://hdl.handle.net/1853/56181
► Intravenous infiltration is a condition wherein an infused solution leaks inadvertently into soft tissue surrounding a hypodermic needle site. This occurrence affects over 10% of…
(more)
▼ Intravenous infiltration is a condition wherein an infused solution leaks inadvertently into soft tissue surrounding a hypodermic needle site. This occurrence affects over 10% of patients in hospitals worldwide, and can lead to severe tissue damage if not treated immediately. However, the methods currently used by medical staff to detect an infiltration are subjective and prone to error. Infiltration becomes an even larger concern in the neonatal intensive care unit, where neonates have a much higher probability of infiltration due to having smaller veins. Unfortunately, infiltrations mostly go unnoticed for an extended period of time due to the neonate's inability to communicate with medical staff. For these reasons, automatic IV infiltration detection could greatly reduce the risk associated with this damaging condition. This dissertation proposes a novel design that uses non-invasive sensing in conjunction with a low-power embedded computing platform to deliver continuous infiltration monitoring around the IV catheter site. This kind of system could be able to detect an infiltration by non-invasively monitoring for known symptoms: swelling of soft tissue, skin cooling, and increased skin firmness; these symptoms can be sensed by measuring skin stretch, temperature, and local bioimpedance. In addition, the system's low-power design and wireless capabilities make it ideal for continuous wear. The proposed automatic IV infiltration detection system could significantly improve the number of infiltrations identified and treated on time.
Advisors/Committee Members: Inan, Omer T (advisor), Hasler, Jennifer O (advisor), Wang, Hua (advisor).
Subjects/Keywords: Intravenous; IV; catheter; venous; vein; soft; tissue; infiltration; extravasation; non-invasive; sensing; automated; detection; neonatal; infant; NICU; intensive; care; hospital; medical; device; embedded; system; wireless; low; power; bioimpedance; electrode; skin; stretch; swelling; edema; formation; strain; gage; temperature; RTD
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jambulingam, J. (2015). Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy. (Masters Thesis). Georgia Tech. Retrieved from http://hdl.handle.net/1853/56181
Chicago Manual of Style (16th Edition):
Jambulingam, Jambu. “Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy.” 2015. Masters Thesis, Georgia Tech. Accessed January 19, 2021.
http://hdl.handle.net/1853/56181.
MLA Handbook (7th Edition):
Jambulingam, Jambu. “Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy.” 2015. Web. 19 Jan 2021.
Vancouver:
Jambulingam J. Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy. [Internet] [Masters thesis]. Georgia Tech; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1853/56181.
Council of Science Editors:
Jambulingam J. Non-Invasive, Multi-Modal Sensing Techniques for Detecting Infiltration During Intravenous Therapy. [Masters Thesis]. Georgia Tech; 2015. Available from: http://hdl.handle.net/1853/56181
27.
Li, Li.
Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy.
Degree: 2013, Erasmus University Medical Center
URL: http://hdl.handle.net/1765/39341
► textabstractLiposomes are nano-sized drug carriers widely used to deliver chemotherapeutic compounds in cancer treatments. While prolonging drug retention in circulation and preventing certain toxic side-effects,…
(more)
▼ textabstractLiposomes are nano-sized drug carriers widely used to deliver chemotherapeutic compounds in cancer treatments. While prolonging drug retention in circulation and preventing certain toxic side-effects, liposomal drugs still need to overcome matters on specific accumulation in the tumor and controlled drug release. The aim of this thesis is to introduce local mild hyperthermia (HT) and thermosensitive liposomes (TSL) to liposomal chemotherapy, to improve therapeutic outcome. At present, two major drug delivery approaches using TSL and mild HT are being proposed. The first approach aims to use mild HT in a two-step approach to first manipulate tumor vasculature, promoting enhanced liposome extravasation, followed by a second mild HT to trigger drug release. A second drug delivery approach is the intravascular drug release approach in which circulating TSL are triggered to rapidly release their contents upon their passage through tumor vasculature. The two-step mild HT approach was less effective compared to intravascular release approach. However, the two-step mild HT approach is an alternative to conventional mild HT, and may be more beneficial to large and deep seated tumors when regional mild HT is required. Our TSL formulation, with an optimized poly(ethylene glycol) (PEG) surface concentration for stability at physiological temperature and fast release at mild HT (41-43°C), further prolonged tumor growth control and improved survival than lyso-lipid based TSL when applied in combination with mild HT.
Subjects/Keywords: hyperthermia; intravital confocal microscopy; liposomal chemotherapy; liposome extravasation; nanoparticles; thermosensitive liposomes; triggered drug release; tumor growth control
…heat mediated-liposomal drug release or
enhanced liposome extravasation, or both. It is clear… …extravasation vs. content release,
using the original thermosensitive liposome formulation of Yatvin… …1) The rate of liposomal extravasation was linearly dependent
upon temperature in the… …doubles for every degree rise in
temperature. In the work of Kong, the liposomal extravasation… …thermal augmentation of liposomal extravasation
occurred up to that size [11]…
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APA (6th Edition):
Li, L. (2013). Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/39341
Chicago Manual of Style (16th Edition):
Li, Li. “Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy.” 2013. Doctoral Dissertation, Erasmus University Medical Center. Accessed January 19, 2021.
http://hdl.handle.net/1765/39341.
MLA Handbook (7th Edition):
Li, Li. “Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy.” 2013. Web. 19 Jan 2021.
Vancouver:
Li L. Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 2013. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1765/39341.
Council of Science Editors:
Li L. Mild Hyperthermia and Thermosensitive Liposomes for Chemotherapy. [Doctoral Dissertation]. Erasmus University Medical Center; 2013. Available from: http://hdl.handle.net/1765/39341
Curtin University of Technology
28.
Gandhi, Neha Sureshchandra.
Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
.
Degree: 2007, Curtin University of Technology
URL: http://hdl.handle.net/20.500.11937/1513
► The Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) has many functions including its roles in leukocyte extravasation as part of the inflammatory response, and in…
(more)
▼ The Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) has many functions including its roles in leukocyte extravasation as part of the inflammatory response, and in the maintenance of vascular integrity through its contribution to endothelial cell-cell adhesion. Various heterophilic ligands of PECAM-1 have been proposed. The possible interaction of PECAM-1 with glycosaminoglycans (GAGs) is the focus of this thesis. The three dimensional structure of the extracellular immunoglobulin (Ig)-domains of PECAM-1 was constructed using homology modelling and threading methods. Potential heparin/heparan sulfate binding sites were predicted on the basis of their amino acid consensus sequences and a comparison with known structures of sulfate binding proteins. Heparin and other GAG fragments have been docked to investigate the structural determinants of their protein binding specificity and selectivity. It is predicted that two regions in PECAM-1 appear to bind heparin oligosaccharides. A high affinity binding region was located in Ig-domains 2 and 3 and a low affinity region was located in Ig-domains 5 and 6.These GAG binding regions are distinct from regions involved in PECAM-1 homophilic interactions. Docking of heparin fragments of different size revealed that fragments as small as a pentasaccharide appear to be able to bind to domains 2 and 3 with high affinity. Binding of longer heparin fragments suggests that key interactions can occur between six sulfates in a hexasaccharide with no further increase in binding affinity for longer fragments. Molecular dynamics simulations were also used to characterise and quantify the interactions of heparin fragments with PECAM-1. These simulations confirmed the existence of regions of high and low affinity for GAG binding and revealed that both electrostatic and van der Waals interactions determine the specificity and binding affinity of GAG fragments to PECAM-1. The simulations also suggested the existence of ‘open’ and ‘closed’ conformations of PECAM-1 around domains 2 and 3.
Subjects/Keywords: heparin/heparan sulfate binding;
glycosaminoglycans (GAGs);
vascular integrity;
leukocyte extravasation;
platelet endothelial cell adhesion molecule 1 (PECAM-1);
extracellular immunoglobulin (Ig);
inflammatory response;
heterophilic ligands
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APA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gandhi, N. S. (2007). Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
. (Thesis). Curtin University of Technology. Retrieved from http://hdl.handle.net/20.500.11937/1513
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gandhi, Neha Sureshchandra. “Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
.” 2007. Thesis, Curtin University of Technology. Accessed January 19, 2021.
http://hdl.handle.net/20.500.11937/1513.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gandhi, Neha Sureshchandra. “Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
.” 2007. Web. 19 Jan 2021.
Vancouver:
Gandhi NS. Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
. [Internet] [Thesis]. Curtin University of Technology; 2007. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/20.500.11937/1513.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gandhi NS. Molecular modelling of platelet endothelial cell adhesion molecule 1 and its interaction with glycosaminoglycans
. [Thesis]. Curtin University of Technology; 2007. Available from: http://hdl.handle.net/20.500.11937/1513
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Sul
29.
Juchem, Beatriz Cavalcanti.
Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem.
Degree: 2014, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/97631
► O Processo de Enfermagem (PE) vem sendo desenvolvido com a utilização de linguagens padronizadas que incluem a classificação diagnóstica da NANDA International, Inc (NANDA-I) e…
(more)
▼ O Processo de Enfermagem (PE) vem sendo desenvolvido com a utilização de linguagens padronizadas que incluem a classificação diagnóstica da NANDA International, Inc (NANDA-I) e as classificações de intervenções e resultados de enfermagem segundo a Nursing Interventions Classification (NIC) e a Nursing Outcomes Classification (NOC), respectivamente. Estudos de validação de conteúdo, por meio de consenso entre peritos, vêm sendo realizados para o refinamento dessas taxonomias e qualificação do cuidado de enfermagem nas diferentes especialidades. Recentemente foi inserido na classificação da NANDA-I o diagnóstico de enfermagem (DE) Risco de reação adversa ao contraste iodado, contemplando as áreas de diagnóstico por imagem. Os objetivos desta pesquisa consistiram em: 1) realizar a validação de conteúdo diagnóstico do DE Risco de reação adversa ao contraste iodado (código 00218), incluindo título, definição, fatores de risco e inserção na estrutura da Taxonomia II da NANDA-I; 2) realizar a validação de conteúdo de resultados de enfermagem da NOC e de intervenções de enfermagem da NIC para o DE em estudo, a partir dos fatores de risco validados como principais. Consistiu em objetivo secundário investigar diferenças entre os grupos de peritos médicos e enfermeiros quanto ao julgamento dos elementos propostos. Para isso, foi utilizado o método de validação de conteúdo diagnóstico de Fehring, que consiste na avaliação de peritos quanto à pertinência de cada item proposto. A partir da opinião dos sujeitos, calcula-se um escore correspondente à média ponderada que determinará se o item foi validado como principal (escore maior ou igual a 0,8), secundário (escore menor do que 0,8 e maior que 0,5) ou não validado (escore igual ou menor que 0,5). A coleta de dados foi realizada por meio de survey eletrônico com perguntas fechadas e espaço para comentários. O grupo de peritos incluiu médicos e enfermeiros com experiência de, no mínimo, cinco anos na assistência a pacientes que se submetem a procedimentos com uso de contraste iodado. A pesquisa compreendeu duas etapas. Na primeira, ocorreu a validação de conteúdo do DE com participação de 74 peritos e, na segunda etapa, foram validados os resultados e intervenções de enfermagem relacionadas ao DE em estudo por 63 peritos. O título foi validado com escore 0,83 e a definição com escore 0,79. Foram apresentados 28 fatores de risco, sendo cinco considerados principais, 22 secundários e um não validado. Os principais e seus escores compreenderam História prévia de reação adversa ao contraste iodado (0,92), Doença renal (0,91), Desidratação (0,86), Uso concomitante de drogas nefrotóxicas (0,82) e Injeção intravascular do contraste iodado (0,81). O único fator não validado correspondeu ao Sexo feminino (0,31), incluído como item de confusão. O DE foi validado com escore total de Validação de Conteúdo Diagnóstico igual a 0,70. A inserção do DE na Taxonomia II da NANDA-I foi avaliada por 41 enfermeiros. O Domínio 11 – Segurança/proteção foi validado com escore 0,84 e a proposta de inserção…
Advisors/Committee Members: Almeida, Miriam de Abreu.
Subjects/Keywords: Diagnostic imaging: nursing; Processos de enfermagem; Diagnostic techniques, cardiovascular: nursing; Diagnóstico de enfermagem; Meios de contraste : Efeitos adversos; Contrast media: adverse effects; Diagnostic extravasation of diagnostic and therapeutic materials: nursing; Nursing diagnosis; Nursing process; Nursing records; Validation studies; Diagnóstico por imagem: enfermería; Técnicas de diagnóstico cardiovascular: enfermería; Medios de contraste: efectos adversos; Extravasación de materiales terapéuticos y diagnósticos: enfermería; Diagnóstico de enfermería; Processos de enfermería; Registros de enfermería; Estudios de validación
Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Juchem, B. C. (2014). Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/97631
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Juchem, Beatriz Cavalcanti. “Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem.” 2014. Thesis, Universidade do Rio Grande do Sul. Accessed January 19, 2021.
http://hdl.handle.net/10183/97631.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Juchem, Beatriz Cavalcanti. “Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem.” 2014. Web. 19 Jan 2021.
Vancouver:
Juchem BC. Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2014. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10183/97631.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Juchem BC. Risco de reação adversa ao contraste iodado : validação de conteúdo diagnóstico, resultados e intervenções de enfermagem. [Thesis]. Universidade do Rio Grande do Sul; 2014. Available from: http://hdl.handle.net/10183/97631
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Puig Saus, Cristina.
Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular.
Degree: 2013, Universitat de Barcelona
URL: http://hdl.handle.net/10803/123285
► Virotherapy with oncolytic adenoviruses holds promise as a new cancer treatment. The main barriers to virotherapy are the poor tumor targeting after systemic administration, the…
(more)
▼ Virotherapy with oncolytic adenoviruses holds promise as a new cancer treatment. The main barriers to virotherapy are the poor tumor targeting after systemic administration, the limited intratumoral dissemination hampered by the fibroblasts, and the extracellular matrix present in the tumors and the antiviral immune response. In this thesis we present different strategies to enhance adenovirus intratumoral dissemination based on optimizing adenovirus release and enhancing its
extravasation and penetration into the tumor.
After multiple rounds of replication in cancer associated fibroblasts (or CAFs) of a mutagenized stock of adenovirus wild type, we isolated an enhanced-release mutant. The mutation responsible for this phenotype, the iLG397T mutation, was identified by sequencing and functional mapping. This mutation enhances adenovirus release and cytotoxicity in CAFs, normal fibroblasts, and a panel of tumor cells. Moreover this mutation increases the antitumor efficacy of the virus in immunodeficient mice with tumors established from tumor cells and mixtures of tumor cells and CAFs.
To increase the potency of the oncolytic adenovirus ICOVIR-15, we introduced the iLG397T mutation into its genome, generating the ICOVIR-15i. This virus also displayed enhanced release, cytotoxicity, and antitumor efficacy in a lung carcinoma model. In Syrian hamsters, an immunocompetent semi-permissive model, only the combination of ICOVIR-15i with gemcitabine shows antitumor activity. This antitumor activity led to a lymphocyte proliferation increase after stimulation with tumor cells, and was not observed when the same experiment was performed in immunodeficient mice. These results suggest that the antitumor efficacy of the combination of ICOVIR-15i and gemcitabine is associated to an immune response against tumor antigens.
We also described the combination of the virus with verapamil, a calcium channel blocker, to enhance adenovirus release, cytotoxicity and antitumor efficacy.
Finally to improve adenovirus
extravasation and penetration into the tumor mass, we inserted the iRGD tumor penetrating peptide into ICOVIR15K capsid. The iRGD peptide enhanced internalization into cells by the interaction with integrins and neuropilin-1, after a proteolytic cleavage by a tumor protease. Integrins and neuropilin-1 are overexpressed in the tumor cells and vasculature. The insertion of the iRGD peptide enhanced tumor transduction, intratumoral dissemination of the virus and antitumor efficacy.
Advisors/Committee Members: Universitat de Barcelona. Facultat de Farmàcia, [email protected] (authoremail), false (authoremailshow), Alemany Bonastre, Ramon (director), Cascalló Piqueras, Manel (director), true (authorsendemail).
Subjects/Keywords: Adenovirus; Adenoviruses; Oncologia; Oncología; Oncology; Tumors; Tumores; Dispersió intratumoral; Dispersión intratumoral; Intratumoral dispersion; Extravasación vascular; Extravasació vascular; Vascular extravasation; Ciències de la Salut; 615
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Puig Saus, C. (2013). Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular. (Thesis). Universitat de Barcelona. Retrieved from http://hdl.handle.net/10803/123285
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Puig Saus, Cristina. “Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular.” 2013. Thesis, Universitat de Barcelona. Accessed January 19, 2021.
http://hdl.handle.net/10803/123285.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Puig Saus, Cristina. “Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular.” 2013. Web. 19 Jan 2021.
Vancouver:
Puig Saus C. Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular. [Internet] [Thesis]. Universitat de Barcelona; 2013. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10803/123285.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Puig Saus C. Estratègies de millora de la potència antitumoral dels adenovirus oncolítics basades en l’increment de l’alliberament viral i l’extravasació vascular. [Thesis]. Universitat de Barcelona; 2013. Available from: http://hdl.handle.net/10803/123285
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations
