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You searched for subject:(exoenzyme). Showing records 1 – 3 of 3 total matches.

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1. Prabhavathy, G. Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;.

Degree: 2015, Manonmaniam Sundaranar University

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Advisors/Committee Members: Rajasekara pandian, M.

Subjects/Keywords: Characterization; exoenzyme; industrially; microorganisms

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Prabhavathy, G. (2015). Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;. (Thesis). Manonmaniam Sundaranar University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/43219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Prabhavathy, G. “Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;.” 2015. Thesis, Manonmaniam Sundaranar University. Accessed September 29, 2020. http://shodhganga.inflibnet.ac.in/handle/10603/43219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Prabhavathy, G. “Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;.” 2015. Web. 29 Sep 2020.

Vancouver:

Prabhavathy G. Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;. [Internet] [Thesis]. Manonmaniam Sundaranar University; 2015. [cited 2020 Sep 29]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/43219.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prabhavathy G. Characterization of industrially important exoenzyme producing microorganisms from bioeffluents;. [Thesis]. Manonmaniam Sundaranar University; 2015. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/43219

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Freie Universität Berlin

2. Pickert, Geethanjali. Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture.

Degree: 2010, Freie Universität Berlin

Hippocampal primary cultures are a widely used model system for studying cell biology and degenerative processes. In the first part, the effect of deltamethrin on the development of hippocampal neuronal cell cultures was investigated. Repeated applications of deltamethrin (between 2 nM and 2000 nM) decreased the number of neurons by 16-40%, respectively. Neuronal death was accompanied by an overall decrease of synaptic proteins. A considerable number of neurons survived treatment with high concentrations of deltamethrin (200-2000 nM) and still displayed characteristics of mature neurons such as synaptic contacts or the expression of members of the Kv1 channel family. When analyzing subtypes of neurons calbindin- as well as somatostatin-positive neurons decreased by 50% after repeated treatment with 2 nM deltamethrin. Under the same conditions neuropeptide Y-positive neurons were up-regulated by 250%. Deltamethrin at concentrations relevant in human toxicology differentially affects survival of neuronal subtypes by exerting either deleterious or supportive effects. In the second part of the study, the effect of C3 ADP-ribosyltransferases, such as Clostridium botulinum (C3bot), Clostridium limosum (C3lim) and Staphylococcus aureus (C3stau2), were studied on the developing hippocampal neurons. Using hippocampal neurones at defined states of differentiation, i have dissected the function of RhoA in axonal and dendritic growth. Inactivation of Rho by C3-catalysed ADP-ribosylation using C3 isoforms (Clostridium limosum, C3lim) or Staphylococcus aureus (C3stau2), diminished axonal branching. By contrast, extracellularly applied nanomolar concentrations of C3 from C. botulinum (C3bot) or enzymatically dead C3bot significantly increased axon growth and axon branching. Extracellular application of enzymatically active or dead C3bot exclusively promotes axonal growth and branching suggesting a novel neurotrophic function of C3 that is independent from its enzymatic activity. Furthermore, low nanomolar concentrations of C3 isoforms (C3bot or enzymatically dead C3bot) significantly promoted process outgrowth and increased process branching in astrocytes. Advisors/Committee Members: [email protected] (contact), w (gender), Prof. Dr. rer. nat. G. Ahnert-Hilger (firstReferee), Prof. Dr. hum. biol. C. Beyer, Priv.-Doz. Dr. rer. nat. K. Scholich (furtherReferee).

Subjects/Keywords: C3- Exoenzyme; Rho; Hippocampus; Deltamethrin; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Pickert, G. (2010). Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13197

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Pickert, Geethanjali. “Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture.” 2010. Thesis, Freie Universität Berlin. Accessed September 29, 2020. https://refubium.fu-berlin.de/handle/fub188/13197.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Pickert, Geethanjali. “Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture.” 2010. Web. 29 Sep 2020.

Vancouver:

Pickert G. Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture. [Internet] [Thesis]. Freie Universität Berlin; 2010. [cited 2020 Sep 29]. Available from: https://refubium.fu-berlin.de/handle/fub188/13197.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pickert G. Effects of deltamethrin and ADP-ribosylation of C3 exoenzymes in the development of hippocampal neurons in culture. [Thesis]. Freie Universität Berlin; 2010. Available from: https://refubium.fu-berlin.de/handle/fub188/13197

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Université du Luxembourg

3. Köhler, Christian. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.

Degree: 2015, Université du Luxembourg

Organic micropollutants with endocrine disruptive properties are present in the aquatic environment. A major part of their emission is caused by municipal wastewater treatment plants (WWTPs). For this reason, a vast amount of research has been undertaken to remove xenobiotics from municipal wastewater by developing post-treatment technologies with some success. However, these technologies cause considerable environmental costs due their high demand for electrical energy implicating an increase in CO2 emissions. Consequently, existing biological treatments need first to be better understood and subsequently optimized regarding xenobiotic removal before post-treatments are employed. The study focused on the fate of xenobiotics during biological wastewater treatment. In particular, metabolic strategies of bacteria degrading pharmaceuticals were investigated within moving bed biofilm reactor (MBBR) processes. Two main objectives were tracked. On the one hand, it was to unfold the impact of macro-substrates in terms of type and molecular complexity on the activity of microorganisms and consequently pharmaceutical degradation performance. On the other hand, the study was set out to explore the adaptation of metabolic means regarding exoenzymes and consortia structure during continuous (long-term) exposure to pharmaceuticals. Accordingly, the ability to increase microbial competences during pharmaceutical short-term pulses was the general target of investigation. Both conditions continuous substance flow and short-term peak loads of xenobiotics are believed to occur in urban WWTPs. A pilot MBBR was set up next to a domestic WWTP. The pilot treated municipal sewage and served as inoculation reservoir for biofilm carriers used for in-depth laboratory experiments. The latter comprised six lab-scale MBBRs featuring flow through operation under controlled conditions regarding temperature, dissolved oxygen, pH, influent flow and influent load. The reactors were conditioned over four weeks with a synthetic sewage providing substrates and micro-nutrients in a similar manner as expected under real conditions. Biofilm was monitored by respirometry and a series of enzyme assays using fluorogenic substrates to capture esterase, phosphatase, alpha- and beta-glucosidase and aminopeptidase activity. All enzymes are essential during organic carbon metabolism. An array of macro-substrates with different molecular complexity was triggering individual enzyme activity profiles. After conditioning, 12 pharmaceuticals being subject to a range of anticipated metabolic pathways and degradation rates were spiked into the MBBRs. Their degradation kinetics were measured by liquid chromatography coupled with tandem mass spectrometry (LC MS/MS). Pseudo first-order kinetics revealed substrate related fingerprints and showed that readily biodegradable substrate leads generally to good pharmaceutical degradation performance compared to synthetic sewage with a mixture of several high molecular organic substrates. The latter was designed to induce the… Advisors/Committee Members: Hansen, Joachim [superviser], Wilmes, Paul [superviser], Vanrolleghem, Peter [superviser], Schosseler, Paul [superviser], Galle, Tom [superviser], Sauter, Thomas [president of the jury].

Subjects/Keywords: pharmaceuticals; xenobiotics; wastewater; wwtp; moving bed biofilm reactor; MBBR; wastewater treatment plant; lab-scale; metabolism; co-metabolism; training; conditioning; 16S rRNA; DNA; RNA; cDNA; enzyme; fingerprinting; consortia; structure; enzyme activity; respirometry; maximum growth rate; SOUR; degradation kinetics; kbiol; macro-substrate; substrate; LC MS/MS; esterase; phosphatase; glucosidase; aminopeptidase; exoenzyme; activity; atenolol; diclofenac; PCA; PCoA; Delftia; Lysobacter; Life sciences :: Biochemistry, biophysics & molecular biology [F05]; Sciences du vivant :: Biochimie, biophysique & biologie moléculaire [F05]; Life sciences :: Biotechnology [F06]; Sciences du vivant :: Biotechnologie [F06]; Life sciences :: Environmental sciences & ecology [F08]; Sciences du vivant :: Sciences de l'environnement & écologie [F08]; Engineering, computing & technology :: Civil engineering [C04]; Ingénierie, informatique & technologie :: Ingénierie civile [C04]; Engineering, computing & technology :: Multidisciplinary, general & others [C99]; Ingénierie, informatique & technologie :: Multidisciplinaire, généralités & autres [C99]

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Köhler, C. (2015). Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. (Doctoral Dissertation). Université du Luxembourg. Retrieved from http://orbilu.uni.lu/handle/10993/22418

Chicago Manual of Style (16th Edition):

Köhler, Christian. “Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.” 2015. Doctoral Dissertation, Université du Luxembourg. Accessed September 29, 2020. http://orbilu.uni.lu/handle/10993/22418.

MLA Handbook (7th Edition):

Köhler, Christian. “Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.” 2015. Web. 29 Sep 2020.

Vancouver:

Köhler C. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. [Internet] [Doctoral dissertation]. Université du Luxembourg; 2015. [cited 2020 Sep 29]. Available from: http://orbilu.uni.lu/handle/10993/22418.

Council of Science Editors:

Köhler C. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. [Doctoral Dissertation]. Université du Luxembourg; 2015. Available from: http://orbilu.uni.lu/handle/10993/22418

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