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1. Franque, Marcos Pinheiro. Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos.

Degree: 2007, Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria

Made available in DSpace on 2016-04-28T20:15:26Z (GMT). No. of bitstreams: 1 2007 - Marcos Pinheiro Franque.pdf: 2601170 bytes, checksum: cef61cb976b6652b0e5f4fe212f81de9 (MD5) Previous issue date: 2007-02-28

Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq

This work aimed the evaluation of biological parameters of parasitic and non-parasitic phases of Rhipicephalus (Boophilus) microplus (= Boophilus microplus) in equine. Four equines were individually infested with approximately 40,000 larvae obteined of R. (B.) microplus females engorged in bovines, being in two of them established three infestations. In the place of larvae fixation, it was noticed development of an intense itchy reaction and the larvae involved in a serum exudates. A larval mortality of approximately 90% was observed, with a small number of larvae changing to nymph stage. The mortality of nymphs was around 60%, with reduction of the itchy reaction, and in the adult stage was observed approximately 30% of mortality. As for the susceptibility, it was observed that two equine were resistant, one moderately resistant and one sensitive to the establishment of infestation by R. (B.) microplus. In the equine considered sensitive, were made observations of parasitic and non-parasitic phase of this ixodid. According to the parameters of the parasitic phase of R. (B.) microplus, of the three experimental infestations, the day at the beginning of detachment of females occurred among infestations were 28 and 31 days, during 12 days at first infestation and 20 days at second and thirst infestation, presentin modal day at 32?; 36? and 37? day, respectively. Were recovered 179 females of R. (B.) microlus at first infestation, 187 and 358 at the second and thirst infestation, corresponding to a mean recovery rate between 0.90 and 1.79%. The means periods of parasitic phase increase among infestations, during between 33. 27 and 38.51 days, being obteined females with mean weight of 90.0mg at the first infestation, 81.5 mg at second and 109.4mg at thirst infestation, considering all females recovered. For the estudy of the non-parasitic phase were selected form each infestation 20, 49, 71 females, presenting means weights of 151.8; 121.1 and 147.8 mg, respectively. The means periods of pre-posture were between 2.8 and 3.5 days. The means periods of posture were of 13.6; 11.7 and 13.4 days, respectively, with pick of posture occurring at the 3rd day after the beginning at three infestations. The means weights of posture were verified between 57.3 and 80.6mg corresponding to 1,146.9 and 1,611.4 eggs produced. The means periods of incubation of eggs produced by R. (B.) microplus studied females occurred between 23.7 and 29 days, presenting hatch means rates decreasing of 67% in the first infestation to 54.7% at the thirst infestation. The means of reprodutive efficiency index also decreased among infestations of 35.5% to 26.9% at the last infestation. It was verified that the means periods between the…

Advisors/Committee Members: Massard, Carlos Luiz, Almeida, Fernando Queiroz.

Subjects/Keywords: Rhipicephalus (Boophilus) microplus; Boophilus microplus; eq?ino; equine; Parasitologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Franque, M. P. (2007). Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos. (Masters Thesis). Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria. Retrieved from https://tede.ufrrj.br/jspui/handle/tede/745

Chicago Manual of Style (16th Edition):

Franque, Marcos Pinheiro. “Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos.” 2007. Masters Thesis, Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria. Accessed April 18, 2021. https://tede.ufrrj.br/jspui/handle/tede/745.

MLA Handbook (7th Edition):

Franque, Marcos Pinheiro. “Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos.” 2007. Web. 18 Apr 2021.

Vancouver:

Franque MP. Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos. [Internet] [Masters thesis]. Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria; 2007. [cited 2021 Apr 18]. Available from: https://tede.ufrrj.br/jspui/handle/tede/745.

Council of Science Editors:

Franque MP. Aspectos biol?gicos de Boophilus microplus (Acari: Ixodidae) mediante infesta??es experimentais em eq?inos. [Masters Thesis]. Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria; 2007. Available from: https://tede.ufrrj.br/jspui/handle/tede/745

2. Meira, Camila T?ngari. Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador.

Degree: 2010, Federal University of Viçosa

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Made available in DSpace on 2015-10-26T13:24:42Z (GMT). No. of bitstreams: 2 camila_tangari_meira.pdf: 312940 bytes, checksum: a4627c11344e9979ceee55d723fadc4a (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2010

Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)

Objetivou-se com este estudo avaliar um conjunto de caracter?sticas morfofuncionais, caracter?sticas morfom?tricas e pontua??o da marcha, atrav?s da an?lise de componentes principais (ACP), e estimar os par?metros gen?ticos, para as caracter?sticas relevantes ap?s an?lise multivariada em cavalos da ra?a Mangalarga Marchador. Dados de 14288 animais, nascidos de 1990 a 2005, foram submetidos ? ACP, objetivando reduzir a dimensionalidade do conjunto de caracter?sticas. Foram consideradas as seguintes caracter?sticas: altura na cernelha, altura na garupa, comprimento da cabe?a, comprimento do pesco?o, comprimento do dorso, comprimento da garupa, comprimento da esp?dua, comprimento do corpo, largura da cabe?a, largura das ancas, per?metro do t?rax, per?metro da canela e a pontua??o da marcha. A partir desta an?lise, sugeriram-se sete vari?veis para descarte, por apresentarem maiores coeficientes de pondera??o, em valor absoluto, a partir do ?ltimo componente principal. Assim, recomendaram-se as seguintes caracter?sticas para serem mantidas em trabalhos que utilizar?o esta mesma base de dados: pontua??o da marcha (PM), altura na garupa (AG), comprimento do dorso (CD), comprimento da garupa (CG), largura da cabe?a (LC) e per?metro da canela (PC). Estas caracter?sticas foram submetidas a uma an?lise gen?tica a fim de estimar suas herdabilidades e correla??es gen?ticas e fenot?picas. Os componentes de (co)vari?ncia necess?rios ? estima??o dos par?metros gen?ticos das caracter?sticas estudadas foram estimados pelo m?todo da M?xima Verossimilhan?a Restrita (REML). O modelo animal multicaracter?stica incluiu efeito gen?tico aditivo direto de animal, como aleat?rio, e os efeitos fixos de grupos contempor?neos, al?m da covari?vel idade do animal ao registro. Altas estimativas de herdabilidade (0,66 para PM a 0,94 para CD) foram encontradas, evidenciando a possibilidade de resposta direta ? sele??o. Foram estimadas correla??es gen?ticas e fenot?picas de ausentes a moderadas magnitudes e discretas tend?ncias gen?ticas ao longo dos anos para maior parte das caracter?sticas avaliadas.

Disserta??o (Mestrado) ? Programa de…

Advisors/Committee Members: Pereira, Idalmo Garcia, Pires, Aldrin Vieira, Fonseca, Ricardo da, Figueiredo, Frederico de Castro, Pires, Aldrin Vieira, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Pereira, Idalmo Garcia.

Subjects/Keywords: An?lise de componentes principais; Correla??es; Descarte de vari?veis; Eq?ino; Herdabilidade

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Meira, C. T. (2010). Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador. (Masters Thesis). Federal University of Viçosa. Retrieved from http://acervo.ufvjm.edu.br:8080/jspui/handle/1/724

Chicago Manual of Style (16th Edition):

Meira, Camila T?ngari. “Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador.” 2010. Masters Thesis, Federal University of Viçosa. Accessed April 18, 2021. http://acervo.ufvjm.edu.br:8080/jspui/handle/1/724.

MLA Handbook (7th Edition):

Meira, Camila T?ngari. “Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador.” 2010. Web. 18 Apr 2021.

Vancouver:

Meira CT. Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador. [Internet] [Masters thesis]. Federal University of Viçosa; 2010. [cited 2021 Apr 18]. Available from: http://acervo.ufvjm.edu.br:8080/jspui/handle/1/724.

Council of Science Editors:

Meira CT. Avalia??o de caracter?sticas morfofuncionais de cavalos da ra?a mangalarga marchador. [Masters Thesis]. Federal University of Viçosa; 2010. Available from: http://acervo.ufvjm.edu.br:8080/jspui/handle/1/724

3. Fernandes, K?tia Roberta. Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887).

Degree: 2007, Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria

Made available in DSpace on 2016-04-28T20:16:24Z (GMT). No. of bitstreams: 1 2007 - K?tia Roberta Fernandes.pdf: 2143619 bytes, checksum: 6ff7127f4642317cf1f53d7aceb93f1f (MD5) Previous issue date: 2007-02-26

Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES

The aim of the present study was to evaluate the natural and experimental infection of B. equi in nymphs and adult Boophilus microplus using optical microscopy and molecular analysis. The experimental infection was observed in nymphs, males and females of B. microplus fed on equines chronically infected with B. equi and in non fed larvae and eggs. For the experiments two equines, of not defined breed, proven to be infected with B. equi were used. The animals were infested with B. microplus larvae of generation F4 obtained from a laboratory maintained population, known to be free of Babesia spp. infection. Daily collections of ticks were realized using as starting point the ecdisys to nymph state. After the collection the hemolymph was examined and the intestine and salivary glands were dissected, smashed on glass slides for microscopy, methanol treated and stained with Giemsa. There were dissected 860 specimens (432 nymphs, 280 females and 148 males). The dissected salivary glands were divided into two parts. The first one was smashed on microscopy glass slides, stained and examined by optical microscopy for morphologic analyses. From the second part was realized DNA extraction and PCR with specific primers for the 18S rRNA. On optical microscopy of nymph hemolymph was possible to be observed the presence of kinetes with claviform aspect characteristic for the genus Babesia. In the salivary glands of B. microplus nymphs the morphology and the sequence of developmental stages of B. equi were observed initiating on day 4 after ecdysis, being possible to see in acinary cells the formation of sporoblasts and ovoid sporozoites measuring 1.5 ?m of diameter and elongated sporozoites with 3.8 to 5.2?m of length and 0.8 to 1.5 ?m of width. The PCR confirmed the presence of B. equi in DNA samples extracted from salivary glands of nymphs, male and female ticks as well as in larvae and eggs. To evaluate the natural infection were collected nymphs and adult B. microplus from two equines naturally infested by these ticks and naturally infected with B. equi. These horses were originated from Seropedica city in the state of Rio de Janeiro. There were dissected 324 specimens (145 nymphs, 138 females and 41 males). The proceedings with the salivary glands were identical to the previously described for the experimental infection. Of the salivary glands submitted to PCR, 70% showed to be infected with B. equi. Microscopical analysis of the salivary glands revealed the presence of sporoblast stages and the development of elongated sporozoites in acinary glands. The morphologic, morphometric and molecular analysis confirmed the experimental and natural infection of the salivary glands of nymphs and adult B. microplus with B.…

Advisors/Committee Members: Massard, Carlos Luiz, Almeida, Fernando Queiroz, Barreira, Jairo Dias.

Subjects/Keywords: Theileria equi; eq?ino; babesiose eq?ina; rea??o em cadeia de polimerase; esporogonia; gl?ndula salivar; vetor; equine babesiosis; horse; polymerase chain; reaction; sporogony; salivary glands; vector; Parasitologia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Fernandes, K. R. (2007). Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887). (Doctoral Dissertation). Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria. Retrieved from https://tede.ufrrj.br/jspui/handle/tede/819

Chicago Manual of Style (16th Edition):

Fernandes, K?tia Roberta. “Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887).” 2007. Doctoral Dissertation, Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria. Accessed April 18, 2021. https://tede.ufrrj.br/jspui/handle/tede/819.

MLA Handbook (7th Edition):

Fernandes, K?tia Roberta. “Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887).” 2007. Web. 18 Apr 2021.

Vancouver:

Fernandes KR. Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887). [Internet] [Doctoral dissertation]. Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria; 2007. [cited 2021 Apr 18]. Available from: https://tede.ufrrj.br/jspui/handle/tede/819.

Council of Science Editors:

Fernandes KR. Biologia, diagn?stico morfol?gico e molecular da infec??o experimental e natural de Babesia equi (Laveran, 1901) em Boophilus microplus (Canestrini, 1887). [Doctoral Dissertation]. Universidade Federal Rural do Rio de Janeiro; Curso de P?s-Gradua??o em Ci?ncias Veterin?rias; UFRRJ; Brasil; Instituto de Veterin?ria; 2007. Available from: https://tede.ufrrj.br/jspui/handle/tede/819

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