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You searched for subject:(epiregulin). Showing records 1 – 2 of 2 total matches.

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University of Adelaide

1. Richani, Dulama. Epidermal growth factor-like peptide signalling and oocyte in vitro maturation.

Degree: 2014, University of Adelaide

A growing body of evidence has recently implicated follicular epidermal growth factor (EGF)-like peptide signalling as essential for the propagation within the ovarian follicle of the LH stimulus that induces oocyte maturation and ovulation. The EGF-like peptides amphiregulin, epiregulin, and betacellulin are produced in mural granulosa and cumulus cells in response to LH, and signal via the EGF receptor (EGFR) in these cells to ultimately induce oocyte maturation, cumulus expansion, and ovulation. Although the function and impact of EGF-like peptide signalling on oocyte maturation in vivo has been characterised, little is currently known about the effect of oocyte in vitro maturation (IVM) on this important signalling network. This thesis aimed to investigate the regulation of EGF-like peptide signalling in mouse cumulus cells and the effect of various IVM models on this network. FSH is a universal IVM additive, and EGF is occasionally used in animal IVM. The effect of FSH-stimulated IVM, EGF-stimulated IVM versus in vivo maturation (IVV) on cumulus cell EGF-like peptide mRNA and/or protein expression, the activity of EGFR, and its classic downstream effector, ERK1/2, were examined. EGF-like peptide mRNA expression, amphiregulin protein expression, and EGFR phosphorylation were significantly lower using FSH-stimulated IVM than during IVV. EGF stimulated significantly lower EGFR phosphorylation, but not EGF-like peptide mRNA expression. These data demonstrate that this signalling network is perturbed in IVM cumulus cells. The effect of FSH, EGF, amphiregulin and epiregulin in IVM on subsequent blastocyst development revealed that epiregulin and amphiregulin significantly increased blastocyst yield and/or the proportion of inner cell mass in blastocysts, than FSH or EGF. Examination of the metabolic profiles of IVM cumulus-oocyte complexes (COCs) matured in the presence of these stimulants revealed that EGF-like peptides and EGF induced significantly higher COC glucose metabolism via the hexosamine biosynthesis pathway than FSH, consequently enabling more hyaluronic acid synthesis and protein β-O-linked glycosylation in the cumulus cells. Epiregulin significantly increased intra-oocyte FAD⁺⁺ and the REDOX ratio compared to FSH, and all three EGF-like peptides induced more oocyte mitochondrial activity than EGF or FSH. Evidence has shown that increasing 3'-5'-cyclic adenosine monophosphate (cAMP) using pharmacological agents significantly increases IVM oocyte developmental competence. This concept, in the form of a pre-IVM culture period with cAMP modulators, was examined in conjunction with IVM in the presence of epiregulin, amphiregulin, EGF or FSH. A pre-IVM phase in conjunction with IVM with EGF-like peptides endowed greater oocyte developmental competence than with FSH or EGF, as evidenced by increased embryo yield and/or quality, which were comparable in embryo development and/or quality rates from IVV oocytes. This thesis provides the physiological basis for, and evidence that, EGF-like peptides are more… Advisors/Committee Members: Gilchrist, Robert Bruce (advisor), Thompson, Jeremy Gilbert E. (advisor), School of Paediatrics and Reproductive Health (school).

Subjects/Keywords: oocyte maturation; amphiregulin; epiregulin; EGF; FSH; IVM

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Richani, D. (2014). Epidermal growth factor-like peptide signalling and oocyte in vitro maturation. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/92804

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Richani, Dulama. “Epidermal growth factor-like peptide signalling and oocyte in vitro maturation.” 2014. Thesis, University of Adelaide. Accessed December 03, 2020. http://hdl.handle.net/2440/92804.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Richani, Dulama. “Epidermal growth factor-like peptide signalling and oocyte in vitro maturation.” 2014. Web. 03 Dec 2020.

Vancouver:

Richani D. Epidermal growth factor-like peptide signalling and oocyte in vitro maturation. [Internet] [Thesis]. University of Adelaide; 2014. [cited 2020 Dec 03]. Available from: http://hdl.handle.net/2440/92804.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Richani D. Epidermal growth factor-like peptide signalling and oocyte in vitro maturation. [Thesis]. University of Adelaide; 2014. Available from: http://hdl.handle.net/2440/92804

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Vanderbilt University

2. Tahaei, Seyedmohammad Ebrahim. Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors.

Degree: PhD, Pharmacology, 2018, Vanderbilt University

Neurofibromatosis type 1 results from mutations in NF1, a gene that encodes Neurofibromin. This common genetic condition is associated with tibial pseudarthrosis (PA), whose etiology is unknown but thought to involve defective bone-repairing osteoprogenitors. The main objective of my thesis was to delineate the causal determinants of the poor osteogenic potential of Nf1-/- osteoprogenitors. I showed that increased Epiregulin and TGFb1 expression does not contribute to the reduced osteogenic differentiation of Nf1-/- osteoprogenitors, and contrary to all expectations, that this phenotype is likely independent from MAPK/ERK constitutive signaling. Using a RNA-Seq approach, I identified changes in pro-inflammatory and extracellular matrix gene signatures as putative determinants of the impaired differentiation of Nf1-/- osteoprogenitors. Finally, I obtained preliminary data pointing to inhibition of RUNX2 activity upon loss of Nf1 function. These results suggest unexpected interactions between Neurofibromin and proximal cell signaling/adhesion components that impact not one but multiple downstream signaling pathways. Advisors/Committee Members: Florent Elefteriou (committee member), Alan Brash (committee member), Ana Carneiro (committee member), Jeffrey Davidson (committee member), Joey Barnett (Committee Chair).

Subjects/Keywords: RNA-Seq; Neurofibromatosis type 1; Bone marrow stroll cells; Differentiation; MAPK signaling; Epiregulin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Tahaei, S. E. (2018). Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/10471

Chicago Manual of Style (16th Edition):

Tahaei, Seyedmohammad Ebrahim. “Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors.” 2018. Doctoral Dissertation, Vanderbilt University. Accessed December 03, 2020. http://hdl.handle.net/1803/10471.

MLA Handbook (7th Edition):

Tahaei, Seyedmohammad Ebrahim. “Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors.” 2018. Web. 03 Dec 2020.

Vancouver:

Tahaei SE. Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors. [Internet] [Doctoral dissertation]. Vanderbilt University; 2018. [cited 2020 Dec 03]. Available from: http://hdl.handle.net/1803/10471.

Council of Science Editors:

Tahaei SE. Molecular Bases of the Reduced Osteogenic Differentiation Potential in Nf1 Deficient Osteoprogenitors. [Doctoral Dissertation]. Vanderbilt University; 2018. Available from: http://hdl.handle.net/1803/10471

.