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1. Obnamia, Jon Albert. Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass.

Degree: 2014, University of Toronto

Maximizing enzymatic hydrolysis performance can be achieved through the combination of experimental work and modeling. The present work utilizes an enzymatic hydrolysis model based on reaction kinetics, Langmuir adsorption isotherms, and product inhibition of enzymes (β-glucosidase, cellobiohydrolase, and endoglucanase). The model was developed from a 10% w/w corn stover system. Glucose yield sensitivity to changes in parameter values was assessed and linked to biomass and enzyme characteristics. A commercial enzyme cocktail (CEC) was subsequently characterized by FPLC and gel electrophoresis to identify key enzymes/activities, and the CEC was used in the enzymatic hydrolysis of 20% w/w steam-exploded hardwood. The model was applied to experimental data from the enzymatic hydrolysis of the steam-exploded hardwood, which provided characteristic reaction rate and inhibition parameters consistent with cellulose and xylan hydrolysis. These model-based analyses enhanced understanding of hydrolysis at commercially relevant solids loadings, while identifying pathways to improve enzyme cocktails and enhance biomass conversion.

MAST

Advisors/Committee Members: Saville, Bradley, Chemical Engineering and Applied Chemistry.

Subjects/Keywords: biofuel; enzymatic hydrolysis; reaction kinetics; modeling; bioconversion; reaction kinetics modeling; enzymatic hydrolysis optimization; 0542

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Obnamia, J. A. (2014). Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/65594

Chicago Manual of Style (16th Edition):

Obnamia, Jon Albert. “Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass.” 2014. Masters Thesis, University of Toronto. Accessed July 20, 2019. http://hdl.handle.net/1807/65594.

MLA Handbook (7th Edition):

Obnamia, Jon Albert. “Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass.” 2014. Web. 20 Jul 2019.

Vancouver:

Obnamia JA. Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2019 Jul 20]. Available from: http://hdl.handle.net/1807/65594.

Council of Science Editors:

Obnamia JA. Modeling the Reaction Kinetics of the Enzymatic Hydrolysis of Lignocellulosic Biomass. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/65594

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