You searched for subject:(endotoxin).
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Colorado State University
1.
Sorrentino, Jennifer Virginia.
Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm.
Degree: MS(M.S.), Environmental and Radiological Health Sciences, 2011, Colorado State University
URL: http://hdl.handle.net/10217/47456 
► The goals of this study are: 1) To optimize an extraction protocol for bulk sheep dust analysis by rFC; 2) To compare the traditional chromogenic…
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▼ The goals of this study are: 1) To optimize an extraction protocol for bulk sheep dust analysis by rFC; 2) To compare the traditional chromogenic LAL to the newer fluorometric rFC assay; 3) To compare the effects of the two most widely used extraction media, pyrogen-free water (PFW) and pyrogen-free water with 0.05% Tween 20 (PFW-Tween 20), on the assay outcomes; and 4) To characterize
endotoxin exposure in a sheep farm environment. Settled and airborne dust samples were collected from a sheep farm for analysis. Settled dust was analyzed with endpoint fluorogenic rFC and kinetic chromogenic LAL in two different laboratories. A total of 34 stationary airborne dust samples (32 matched) were collected with Institute of Occupational Medicine (IOM) inhalable dust samplers, using polyvinyl chloride (PVC) filters. Airborne samples were analyzed with endpoint fluorogenic rFC and endpoint chromogenic LAL in the same laboratory. The results of the bulk dust analysis showed a significant difference in polystyrene (PS) tubes and polypropylene (PP) tubes used for extraction indicating that PS tubes yielded higher
endotoxin levels (t = 3.82, p = 0.000). No difference in
endotoxin levels was found for centrifugation and spike recovery was closest to 100% for extraction with PFW. The results of the airborne dust study showed a strong positive correlation between the rFC and LAL assays with PFW-Tween 20 extraction. All samples extracted in PFW-Tween 20 had a higher
endotoxin recovery compared with those extracted in PFW for both the rFC and LAL assays.
Advisors/Committee Members: Reynolds, Stephen J. (advisor), Keefe, Thomas J. (committee member), Goodridge, Lawrence D. (committee member).
Subjects/Keywords: endotoxin
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APA (6th Edition):
Sorrentino, J. V. (2011). Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/47456
Chicago Manual of Style (16th Edition):
Sorrentino, Jennifer Virginia. “Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm.” 2011. Masters Thesis, Colorado State University. Accessed January 22, 2021.
http://hdl.handle.net/10217/47456.
MLA Handbook (7th Edition):
Sorrentino, Jennifer Virginia. “Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm.” 2011. Web. 22 Jan 2021.
Vancouver:
Sorrentino JV. Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10217/47456.
Council of Science Editors:
Sorrentino JV. Comparison of the limulus amebocyte lysate (LAL) and recombinant factor C (rFC) endotoxin bioassays: characterization of aerosols and settled dust on a sheep farm. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/47456
McGill University
2.
Sánchez-Cantú, Leopoldo.
The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock.
Degree: MS, Department of Surgery, 1990, McGill University
URL: https://escholarship.mcgill.ca/downloads/tt44pq37w.pdf
;
https://escholarship.mcgill.ca/concern/theses/3197xp04z
► Lethality, weight loss and Tumor Necrosis Factor (TNF) production induced by lypopolysaccharide (I.PS) were studied in rats during early and late endotoxin tolerance. Different 0-speclfic…
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▼ Lethality, weight loss and Tumor Necrosis Factor (TNF) production induced by lypopolysaccharide (I.PS) were studied in rats during early and late endotoxin tolerance. Different 0-speclfic types of I.PS were used. The TNF serum profile in two models of sepsis and the effect of I.PS tolerance in the lethality induced by these models were also investigated. Results show diminished LPS-induced TNF levels during early I.PS tolerance either elicited with the same or a different type of LPS. No correlation was found between TNF levels and LPS-induced mortality in naive animals. LDw toxicity I.PS preparations induced TNF levels similar to levels induced with toxic types of I.PS. Late tolerance was associated with low levels of LPS-induced TNF and similar susceptibility to the lethal effect as naive rats. Early endotoxin tolerance conferred high protection from mortality induced with live intra-peritoneal bacteria or caecalligation and puncture. In these last two animaJ models of sepsis, high serum levels of TNF were not detected.
La mortalité, la perte de poids, et la production de TNF ont été étudiées sur des rats rendus tolérants à différents serotypes O d'endotoxine. Dans deux modèles de sepsis, les taux sériques de TNF et les effets de la tolérance au LPS sur la mortalité ont été investigués. Les résultats montrent une diminution de la réponse de TNF induite par LPS durant la phase précoce de tolérance à LPS, qu'on ait utilise un type identique ou différent de LPS. Il n'y avait pas de corrélation entre les taux de TNF et la mortalité LPS-induite. Les LPS de faible toxicité et les LPS hautenient toxiques ont entrainés des taux de TNF similaires. La tolérance tardive était associée à de faibles taux de TNF LPS-induit, ainsi qu'l un effet lethal identique à celui observé chez des rats intacts. La tolérance précoce a protégé de la mortalité dans les deux modèles utilisés. Dans ces modèles de sepsis, une production de TNF n'a pas pu être démontrée.
Advisors/Committee Members: Christou, N. V. (Supervisor).
Subjects/Keywords: Endotoxin
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APA (6th Edition):
Sánchez-Cantú, L. (1990). The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock. (Masters Thesis). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/tt44pq37w.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp04z
Chicago Manual of Style (16th Edition):
Sánchez-Cantú, Leopoldo. “The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock.” 1990. Masters Thesis, McGill University. Accessed January 22, 2021.
https://escholarship.mcgill.ca/downloads/tt44pq37w.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp04z.
MLA Handbook (7th Edition):
Sánchez-Cantú, Leopoldo. “The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock.” 1990. Web. 22 Jan 2021.
Vancouver:
Sánchez-Cantú L. The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock. [Internet] [Masters thesis]. McGill University; 1990. [cited 2021 Jan 22].
Available from: https://escholarship.mcgill.ca/downloads/tt44pq37w.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp04z.
Council of Science Editors:
Sánchez-Cantú L. The role of endotoxin and tumor necrosis factor in the pathogenesis of septic shock. [Masters Thesis]. McGill University; 1990. Available from: https://escholarship.mcgill.ca/downloads/tt44pq37w.pdf ; https://escholarship.mcgill.ca/concern/theses/3197xp04z
University of Illinois – Chicago
3.
Pang, Jingbo.
Galectin-3 in Obesity and Endotoxin-Induced Inflammation.
Degree: 2016, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21593
► Obesity has become a worldwide epidemic that causes both health and economic burdens. The mild but persistent elevation of the bacterial product lipopolysaccharide (LPS) in…
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▼ Obesity has become a worldwide epidemic that causes both health and economic burdens. The mild but persistent elevation of the bacterial product lipopolysaccharide (LPS) in the systemic circulation contributes to increased risk and progression of many obesity-associated chronic pathologies. Galectin-3 (Gal3), a mediator of inflammation, is up-regulated in obesity and regulates LPS-induced inflammation, while LPS also influences Gal3 production both in vivo and in vitro. Therefore, we designed this study to investigate the association between Gal3 with markers of adiposity and inflammation in metabolically healthy women who are predominantly obese and African-American, as well as the reciprocal regulation of Gal3 and LPS in mice.
We found that serum Gal3 levels were significantly higher in obese women compared to the non-obese individuals but comparable among different classes of obesity. Markers of adiposity and inflammation significantly and positively influenced Gal3 levels only in women with low/moderate risk of cardiovascular disease (CVD). In mice, Gal3 deficiency led to induction of significantly lower circulating cytokines and hepatic acute-phase response caused by non-lethal doses of LPS administration. Additionally, inhibition of extracellular Gal3 did not replicate the protective effects of Gal3 mentioned above, which were specific for LPS (TLR4)-induced inflammation, as we observed opposite outcomes in Poly I:C (TLR3) injected mice. Moreover, LPS differentially affected protein production versus gene expression of Gal3 both in vivo and in vitro. Finally, repeated injections of ultra-low doses of LPS, which mimics the mild endotoxemia in obesity, induced a trend towards increased adiposity and development of mild hyperglycemia, as well as increased hepatic acute-phase response in wild-type mice. While those LPS-induced alterations were either less remarkable, or absent in Gal3 knockout mice.
Together, our findings indicate that Gal3 positively associates with adiposity and inflammation in women, while presence of elevated CVD risk may weaken the strength of Gal3 as a biomarker of inflammation. Additionally, Gal3 specifically promotes LPS-induced inflammation in mice, as Gal3 deficiency protects mice from both non-lethal and repeated ultra-low doses of LPS-induced inflammation, while LPS also affects production of Gal3 in a complex manner.
Advisors/Committee Members: Fantuzzi, Giamila (advisor), Braunschweig, Carol (committee member), Koh, Timothy J (committee member), Song, Zhenyuan (committee member), Liew, Chong Wee (committee member), Fantuzzi, Giamila (chair).
Subjects/Keywords: Obesity; Inflammation; Endotoxin
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APA (6th Edition):
Pang, J. (2016). Galectin-3 in Obesity and Endotoxin-Induced Inflammation. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21593
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pang, Jingbo. “Galectin-3 in Obesity and Endotoxin-Induced Inflammation.” 2016. Thesis, University of Illinois – Chicago. Accessed January 22, 2021.
http://hdl.handle.net/10027/21593.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pang, Jingbo. “Galectin-3 in Obesity and Endotoxin-Induced Inflammation.” 2016. Web. 22 Jan 2021.
Vancouver:
Pang J. Galectin-3 in Obesity and Endotoxin-Induced Inflammation. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10027/21593.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pang J. Galectin-3 in Obesity and Endotoxin-Induced Inflammation. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/21593
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
Doxaki, Christina.
Regulation of microRNAs during activation of macrophages.
Degree: 2016, University of Crete (UOC); Πανεπιστήμιο Κρήτης
URL: http://hdl.handle.net/10442/hedi/37158
► Endotoxin tolerance occurs to protect the organism from hyperactivation of innate immune responses, primarily mediated by macrophages. Regulation of endotoxin tolerance occurs at multiple levels…
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▼ Endotoxin tolerance occurs to protect the organism from hyperactivation of innate immune responses, primarily mediated by macrophages. Regulation of endotoxin tolerance occurs at multiple levels of cell responses and requires significant changes in gene expression. During macrophage activation, induced expression of miR-155 and miR-146a contributes to the regulation of the inflammatory response and endotoxin tolerance. Herein, we demonstrate that expression of both miRNAs is co-ordinately regulated during endotoxin tolerance by a complex mechanism involving mono-allelic inter-chromosomal association, alterations in histone methyl marks and transcription factor binding. Upon activation of naïve macrophages, Histone3 was tri-methylated at lysine4 (H3K4me3) and NFBp65 was bound on both miR-155 and miR-146a gene loci. However, at the stage of endotoxin tolerance both miR gene loci were occupied by C/EBPβ, NFBp50 and the repressive Histone3 marks H3K9me3. DNA fluorescence in situ hybridization (DNA-FISH) experiments revealed mono-allelic inter-chromosomal co-localization of miR-155 and miR-146a gene loci at the stage of endotoxin tolerance, while RNA-DNA-FISH experiments showed that the co-localized alleles were silenced, suggesting a common repressive mechanism. Genetic ablation of Akt1, which is known to abrogate endotoxin tolerance, abolished induction of loci co-localization and C/EBPβ binding, further supporting that this mechanism occurs specifically in endotoxin tolerance. This thesis demonstrates that two miRNAs are co-ordinately regulated via gene co-localization at the three dimensional chromatin space, similar transcriptional machinery and Histone3 methylation profile, contributing to the development of endotoxin tolerance. Further insight into the role of AKT in regulation of M1/M2 polarization, revealed the essential role of these microRNAs in macrophage phenotype. Akt1 ablation promotes miR-155 expression in LPS-stimulated macrophage. Measuring miR-155 in Akt2-depleted macrophages revealed that Akt2 ablation had the opposite effect, reducing miR-155 expression in both resting and LPS-activated macrophages. Therefore, down-regulation of miR-155 in Akt2-defiecient macrophages results in up-regulation of its target C/EBPβ and, consequently, in the induction of Arg1, a hallmark of M2 macrophage polarization. Akt2 deficiency resulted, however, in a significant upregulation of miR-146a, which mediates M1 phenotype suppression and assure endotoxin tolerance. miR-146a transfection in WT macrophages was able to inhibit iNOS induction while miR-146a suppression in Akt2-depleted mice resulted in upregulation of iNOS expression. The physiological and clinical significance of these miRs in sepsis was supported by further data in humans. Critically ill patients with impaired immune responses (CARS syndrome) are associated with increased miR-155 and miR-146 expression. In vivo transferring of these miRs by using amphoteric liposomes seems to be highly promising, underlining miR-155 and miR-146 as potential novel molecular…
Subjects/Keywords: Μακροφάγα; endotoxin tolerance
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APA (6th Edition):
Doxaki, C. (2016). Regulation of microRNAs during activation of macrophages. (Thesis). University of Crete (UOC); Πανεπιστήμιο Κρήτης. Retrieved from http://hdl.handle.net/10442/hedi/37158
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Doxaki, Christina. “Regulation of microRNAs during activation of macrophages.” 2016. Thesis, University of Crete (UOC); Πανεπιστήμιο Κρήτης. Accessed January 22, 2021.
http://hdl.handle.net/10442/hedi/37158.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Doxaki, Christina. “Regulation of microRNAs during activation of macrophages.” 2016. Web. 22 Jan 2021.
Vancouver:
Doxaki C. Regulation of microRNAs during activation of macrophages. [Internet] [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2016. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10442/hedi/37158.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Doxaki C. Regulation of microRNAs during activation of macrophages. [Thesis]. University of Crete (UOC); Πανεπιστήμιο Κρήτης; 2016. Available from: http://hdl.handle.net/10442/hedi/37158
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
5.
Anthonisse, M.I.
The Effect of Ionization on the Air Quality in a Horse Stable.
Degree: 2014, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/297097
► Horses kept in stables are likely to be exposed to high levels of organic dust. Organic dust plays a role in increased risk of inflammatory…
(more)
▼ Horses kept in stables are likely to be exposed to high levels of organic dust. Organic dust plays a role in increased risk of inflammatory reactions and is associated with respiratory diseases. There are multiple ways to decrease the amount of organic dust in the horse stable, of which the most common are a change in bedding and feeding regime and an increase in natural ventilation. The aim of this study was to investigate the effect of the ionization of air on dust,
endotoxin and fungus in horse stables , and the differences between those levels in stables with horses kept on shavings eating haylage and stables with horses kept on straw eating dry hay.
Four units (96m2 each) of six boxes were used. Each unit was equipped with an ionization installation, composed of a ionizationbox connected to electricity network, 3 corona wires and 4 earthed wires (all wires had a length of 12m). In 2 units, horses were kept on shavings and were fed haylage and in the other 2 units horses were kept on straw and were fed dry hay. Ambient inhalable dust samples were collected on a fixed, similar position within each unit. Samples were taken in each unit with and without activated ionization and during daytime and during nighttime, repeatedly over the course of a week. In addition, sampling was performed on 5 different positions in each stable unit to verify if there are locations within stable units that have consistent higher dust levels than other locations. The dust samples were analyzed for
endotoxin by the Limulus Amebocyte Lysate assay. Culturable fungi were collected with an Anderson impactor on DG-18 Agar plates, with a sampling time of 30 seconds per sample.
156 samples were examined for dust levels and 154 samples were examined for
endotoxin levels. Highest dust and endotoxins levels were found in the units where the horses were kept on straw, being fed dry hay. Comparing day and night, higher dust and endotoxins levels were found during daytime. 86 samples were taken for the examination of growth of fungal colonies. Samples taken in straw units showed higher growth of fungal colonies than samples taken in units where horses were kept on shavings. Nighttime sampling showed less fungal growth than daytime sampling.
No difference in dust, endotoxins or fungal growth were found when ionization was activated. Dust, endotoxins and fungal spores do tend to be significantly lower in stables where horses are being kept on shavings, being fed with haylage, compared to stables where they are being kept on straw and being fed with hay.
Advisors/Committee Members: Westermann, C.M., van Eerdenburg, F.J.C.M., Wouters, I.M..
Subjects/Keywords: Ionization; dust; endotoxin; fungus
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APA (6th Edition):
Anthonisse, M. I. (2014). The Effect of Ionization on the Air Quality in a Horse Stable. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/297097
Chicago Manual of Style (16th Edition):
Anthonisse, M I. “The Effect of Ionization on the Air Quality in a Horse Stable.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/297097.
MLA Handbook (7th Edition):
Anthonisse, M I. “The Effect of Ionization on the Air Quality in a Horse Stable.” 2014. Web. 22 Jan 2021.
Vancouver:
Anthonisse MI. The Effect of Ionization on the Air Quality in a Horse Stable. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/297097.
Council of Science Editors:
Anthonisse MI. The Effect of Ionization on the Air Quality in a Horse Stable. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/297097
6.
Cavalli, Daiana [UNESP].
Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas.
Degree: 2017, Universidade Estadual Paulista
URL: http://hdl.handle.net/11449/148838
► Os objetivos deste trabalho são: 1) Quantificar por checkerboard a carga microbiana e pelo método de LAL endotoxinas (EU/mL) nas infecções endodônticas primárias; 2) Realizar…
(more)
▼ Os objetivos deste trabalho são: 1) Quantificar por checkerboard a carga microbiana e pelo método de LAL endotoxinas (EU/mL) nas infecções endodônticas primárias; 2) Realizar o monitoramento dos níveis de endotoxinas (EU/mL) e de carga microbiana antes do tratamento, após o preparo biomecânico com sistemas de instrumentação rotatória, reciprocante e híbrida e após o uso da medicação intracanal; 3) Relacionar sinais e sintomas clínicos com níveis de endotoxinas, micro-orgnismos e com complexos bacterianos; 4) Relacionar volumetria dos canais radiculares por meio de TCFC com níveis de endotoxina, micro-organismos e complexos bacterianos. Trinta dentes com infecção endodôntica primária e presença de lesão periapical foram submetidos a TCFC antes do tratamento e avaliados quanto a presença de sinais e sintomas clínicos. Após abertura coronária, foi realizada a coleta inicial nos canais radiculares, e em seguida, procedeu-se com o tratamento endodôntico, sendo os dentes divididos em diferentes grupos experimentais de acordo com o sistema de instrumentação utilizado (n=10): rotatório Mtwo (MTWO), reciprocante Reciproc (REC), e híbrido Genius (GEN). Durante o preparo biomecânico, os canais foram irrigados com 24 mL de NaOCl 2,5%. Foram realizadas coletas do conteúdo dos canais radiculares: logo após a abertura coronária (1 col), após a instrumentação (2 col), e após a MIC por 14 dias, realizada com pasta de hidróxido de cálcio associada a solução salina fisiológica (3 col). A detecção de micro-organimos foi realizada pelo teste checkerboard DNA-DNA hybridization. A quantificação de endotoxinas foi realizada pelo teste cinético cromogênio do lisado de amebócito de Limulus. As volumetrias dos canais radiculares foram realizadas com auxílio do software Nemotec®. Todos os dados foram analisados estatisticamente. Os resultados mostraram a detecção de micro-organismos e endotoxinas em 100% das amostras iniciais, sendo as bactérias C. ochracea e F. nucleatum as mais prevalentes (53%). Após o PBM, os micro-organismos mais encontrados foram F. nucleatum e L. buccalis (50%); e após a MIC C. gracilis (53,3%). Não houve diferença estatística entre os grupos quanto à redução da carga microbiana. Quanto as endotoxinas, logo após o PBM, o grupo que mais reduziu foi o MTWO, seguido por GEN e REC; após a MIC, o grupo que mais reduziu foi o GEN, seguido pelo MTWO e REC, porém todos os grupos se comportaram de maneira semelhante. Dor espontânea foi relacionada com P. nigrescens; dor a percussão com P. gingivalis, V. parvula, S. sputigena, P. nigrescens e E. saburreum; presença de fístula foi relacionada com o complexo laranja, Gram-positivas e anaeróbios facultativos, e micro-organismos E. corrodens, P. micra, C. showae e E. saburreum. O maior volume do canal radicular foi correlacionado fortemente com anaeróbios estritos, com o complexo laranja e o micro-organismo P. micra. O PBM foi efetivo na redução de bactérias e endotoxinas do canal radicular, mas sem diferença estatística entre os três sistemas utilizados. Conclui-se que o PBM com…
Advisors/Committee Members: Garakis, Marcia Carneiro Valera [UNESP], Cardoso, Flávia Goulart da Rosa [UNESP], Universidade Estadual Paulista (UNESP).
Subjects/Keywords: Endotoxina; Instrumentação; Microbiota; Endotoxin; Instrumentation
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APA (6th Edition):
Cavalli, D. [. (2017). Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/148838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cavalli, Daiana [UNESP]. “Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas.” 2017. Thesis, Universidade Estadual Paulista. Accessed January 22, 2021.
http://hdl.handle.net/11449/148838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cavalli, Daiana [UNESP]. “Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas.” 2017. Web. 22 Jan 2021.
Vancouver:
Cavalli D[. Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas. [Internet] [Thesis]. Universidade Estadual Paulista; 2017. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/11449/148838.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cavalli D[. Estudo comparativo de sistemas rotatório, reciprocante e híbrido no preparo de canais radiculares em dentes com infecção endodôntica primária: perfil microbiano e quantificação de endotoxinas. [Thesis]. Universidade Estadual Paulista; 2017. Available from: http://hdl.handle.net/11449/148838
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Colorado State University
7.
Funk, Sara.
Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor.
Degree: MS(M.S.), Environmental and Radiological Health Sciences, 2011, Colorado State University
URL: http://hdl.handle.net/10217/47272
► This pilot study measured the impact of seasonal ventilation changes on concentrations of organic dust, endotoxin and bacteria in one modern dairy milking parlor. Pyrosequencing,…
(more)
▼ This pilot study measured the impact of seasonal ventilation changes on concentrations of organic dust,
endotoxin and bacteria in one modern dairy milking parlor. Pyrosequencing, a new non-target specific molecular methodology was used to characterize airborne bioaerosols. Area samples for inhalable dust, respirable dust,
endotoxin, and bacteria were collected in one modern dairy parlor during both summer and winter seasons. Five sampling sessions were performed at approximately weekly intervals during each season. The summer season included an open facility with fresh mechanical air ventilation. The winter season consisted of a closed facility with no fresh air ventilation and forced heat. Aerosol size distributions, air velocity inside the parlor, humidity, temperature, and CO2 were also sampled on each trip. Two-way ANOVA was performed to test statistically significant differences between variables. No significant differences between mean concentrations of inhalable dust, respirable dust, or bacteria were seen by season.
Endotoxin showed a near significant difference (p=0.06). CO2 concentration doubled during the winter season as compared to the summer (p<0.001) due to reduced ventilation. The aerosol size distribution did not vary between seasons (MMOD=12µm) indicating that seasonal ventilation changes do not affect the particle size distribution. The most common bacterial genera in both seasons were Clostridium (anaerobic), Oscillibacter and Staphylococcus - all Gram positive bacteria. Gram-positive bacterial genera occurred more frequently during the summer than winter seasons. Gram-negative bacteria cell counts increased in the winter season. Average concentrations of total bacteria in the facility during summer and winter were 2839 and 7008 counts/m3, respectively. This study was the first to apply Pyrosequencing to measure bioaerosols in a dairy environment. The diversity of bacteria and predominance of Gram-positive bacteria is consistent with studies in swine and poultry facilities. Concentrations of dust,
endotoxin, and bacteria were low during both seasons in this milking parlor, most likely due to the new construction of the facility. These study results will inform the design of future comprehensive studies of aerosol exposure interventions in dairy operations. Understanding these aerosol exposures and potential interventions is important for reducing respiratory disease among workers in the dairy industry.
Advisors/Committee Members: Reynolds, Stephen J. (advisor), Roman-Muniz, Noa (committee member), Volckens, John (committee member).
Subjects/Keywords: bacteria; pyrosequencing; endotoxin; dust; dairy
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APA (6th Edition):
Funk, S. (2011). Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/47272
Chicago Manual of Style (16th Edition):
Funk, Sara. “Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor.” 2011. Masters Thesis, Colorado State University. Accessed January 22, 2021.
http://hdl.handle.net/10217/47272.
MLA Handbook (7th Edition):
Funk, Sara. “Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor.” 2011. Web. 22 Jan 2021.
Vancouver:
Funk S. Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10217/47272.
Council of Science Editors:
Funk S. Evaluation of seasonal ventilation changes and their effect on ambient dust, endotoxin and bioaerosol concentrations in a dairy parlor. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/47272
Virginia Tech
8.
Guedes Alves da Silva, Adriana.
The Role of the CD14 molecule in equine endotoxemia.
Degree: MS, Veterinary Medical Sciences, 2012, Virginia Tech
URL: http://hdl.handle.net/10919/76807
► Objectives - To evaluate the effects of equine sCD14 and monoclonal antibodies (mAbs) to equine CD14 on LPS-induced TNF° expression of equine peripheral blood mononuclear…
(more)
▼ Objectives - To evaluate the effects of equine sCD14 and monoclonal antibodies (mAbs) to equine CD14 on LPS-induced TNF° expression of equine peripheral blood mononuclear cells (PBMCs). To determine serum concentrations of soluble (sCD14) in a population of horses with gastrointestinal diseases or other illnesses likely to result in endotoxemia; and identify relationships with clinical data.
Animals - Part 1; 10 healthy horses. Part 2; 55 clinical cases and 23 healthy control horses.
Procedure - Part 1; PBMCs were incubated with Escherichia coli LPS, CD14 mAb, sCD14, CD14 mAb plus E coli LPS or sCD14 plus E coli LPS. Supernatants were collected at 6 hours and assayed for tumor necrosis factor ° (TNF°) activity. Part 2; Serum sCD14 was measured at admission and then at 24 and 48 hours after admission using a bead-based multiplex assay.
Results - Part 1; Pre-incubation with CD14 mAb did not inhibit LPS-induced TNF° protein production in isolated equine monocytes. Use of sCD14 inhibited LPS-induced TNF° protein production in isolated monocytes in a concentration-dependent manner. Part 2; Serum concentration of sCD14 was positively related to duration of clinical signs (P = 0.007), respiratory rate (P=0.04) and band neutrophil count (P = 0.0002). There was no correlation between serum concentration of sCD14 and heart rate, temperature, hematocrit, lactate, white blood cell count, fibrinogen, creatinine, urea nitrogen, glucose and anion gap values. Serum sCD14 did not correlate with outcome at any time point for clinical cases.
Advisors/Committee Members: Furr, Martin O. (committeechair), Desrochers, Anne M. (committee member), McKenzie, Harold C. III (committee member).
Subjects/Keywords: soluble CD14; horse; endotoxin
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APA (6th Edition):
Guedes Alves da Silva, A. (2012). The Role of the CD14 molecule in equine endotoxemia. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/76807
Chicago Manual of Style (16th Edition):
Guedes Alves da Silva, Adriana. “The Role of the CD14 molecule in equine endotoxemia.” 2012. Masters Thesis, Virginia Tech. Accessed January 22, 2021.
http://hdl.handle.net/10919/76807.
MLA Handbook (7th Edition):
Guedes Alves da Silva, Adriana. “The Role of the CD14 molecule in equine endotoxemia.” 2012. Web. 22 Jan 2021.
Vancouver:
Guedes Alves da Silva A. The Role of the CD14 molecule in equine endotoxemia. [Internet] [Masters thesis]. Virginia Tech; 2012. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10919/76807.
Council of Science Editors:
Guedes Alves da Silva A. The Role of the CD14 molecule in equine endotoxemia. [Masters Thesis]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/76807
Virginia Tech
9.
Denko, Laura Michelle.
Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin.
Degree: MS, Human Nutrition, Foods, and Exercise, 2012, Virginia Tech
URL: http://hdl.handle.net/10919/76808
► Obesity-related metabolic derangements have been linked to toll-like receptor 4 (TLR4), an innate immune system receptor, due to its role in proinflammatory pathways. Lipopolysaccharide (LPS),…
(more)
▼ Obesity-related metabolic derangements have been linked to toll-like receptor 4 (TLR4), an innate immune system receptor, due to its role in proinflammatory pathways. Lipopolysaccharide (LPS), a gram-negative bacteria cell wall component, is the ligand for TLR4, and has been shown to be elevated in states of metabolic disease. Heightened levels of circulating
endotoxin is termed metabolic endotoxemia and has been linked to systemic inflammation which is associated with obesity, type 2 diabetes mellitus (T2DM), and cardiovascular disease (CVD). Immune cells exhibit a protective ability to develop
endotoxin tolerance. The objective of this study was to determine if
endotoxin tolerance exists in skeletal muscle cells, and if a condition that mimics a state of over nutrition, such as elevated levels of fatty acids, affect this tolerance. To this end, L6 skeletal muscle cells were treated with low (50 pg/mL)- and high (500 ng/mL)-doses of LPS, with and without the presence of free fatty acids (FFAs). Tolerance was assessed by measuring: 1) changes in mRNA expression of interleukin-6 (IL-6) and monocyte chemoattractant-1 (MCP-1) as markers of a pro-inflammatory response; and 2) mRNA levels of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1-°) and mitochondrial oxidative capacity via an XF24 Flux Analyzer (Seahorse Bioscience) as measures of the metabolic response. Tolerance to LPS was observed in response to low- and high-doses with MCP-1 mRNA transcription but not IL-6. Changes in PGC1-° and mitochondrial OCR exhibited a tolerant effect in response to the high dose of LPS but not the low dose. The addition of free fatty acids to LPS treatments did not prevent the tolerant effects under any conditions. In conclusion, LPS tolerance exists in skeletal muscle cells but appears to differ depending on pro-inflammatory target and LPS concentration. Additionally, fatty acids, in the current model, have no effect on LPS tolerance.
Advisors/Committee Members: Hulver, Matthew W. (committeechair), Davy, Kevin P. (committee member), Li, Liwu (committee member), Frisard, Madlyn I. (committee member).
Subjects/Keywords: TLR4; endotoxin; LPS; skeletal muscle
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APA (6th Edition):
Denko, L. M. (2012). Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/76808
Chicago Manual of Style (16th Edition):
Denko, Laura Michelle. “Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin.” 2012. Masters Thesis, Virginia Tech. Accessed January 22, 2021.
http://hdl.handle.net/10919/76808.
MLA Handbook (7th Edition):
Denko, Laura Michelle. “Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin.” 2012. Web. 22 Jan 2021.
Vancouver:
Denko LM. Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin. [Internet] [Masters thesis]. Virginia Tech; 2012. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10919/76808.
Council of Science Editors:
Denko LM. Cellular Reprogramming in Skeletal Muscle after Repeated Exposures to Endotoxin. [Masters Thesis]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/76808
Virginia Tech
10.
Stevens, Joseph R.
The Effects of Low Dose Endotoxin on Glucose Homeostasis.
Degree: PhD, Human Nutrition, Foods, and Exercise, 2014, Virginia Tech
URL: http://hdl.handle.net/10919/64849
► Obese individuals present with an increased inflammatory tone as compared to healthy, normal-weight individuals, which is associated with insulin resistance. One factor hypothesized to contribute…
(more)
▼ Obese individuals present with an increased inflammatory tone as compared to healthy, normal-weight individuals, which is associated with insulin resistance. One factor hypothesized to contribute to increased inflammation in obese and diabetic states is elevated blood
endotoxin levels, also known as metabolic endotoxemia. In healthy rodents (non-obese and insulin sensitive), there is evidence that blood
endotoxin levels fluctuate over the course of the day with elevations in the post-prandial state that return to baseline levels in the post-absorptive state. High-fat feeding in these animals altered these fluctuations causing
endotoxin levels to remain high throughout the day. The effects of alterations in
endotoxin levels on glucose metabolism are not understood. The goal of this study was to determine the effects of short-term and long-term increases in
endotoxin of a low magnitude on insulin signaling in a human primary cell line as well as the effects of short-term
endotoxin treatments on glucose homeostasis in a C57/Bl6 mouse model. First, we tested the hypothesis in cell culture that short-term low-dose
endotoxin treatments would enhance insulin-signaling and glycogen synthesis while long-term treatments would have inhibitory effects. Under our second hypothesis, we examined whether short-term low-dose treatments of
endotoxin would contribute to improvements in glucose tolerance in a mouse model. In contrast to our first hypothesis, short-term
endotoxin treatments did not improve insulin signaling or glycogen synthesis although long-term treatments did contribute to decreases in glycogen synthesis. Interestingly, short-term
endotoxin treatments resulted in significant improvements in glucose clearance in the mouse model; this is believed to be partly attributed to LPS inhibiting gluconeogenesis. Future studies are necessary to understand the mechanisms responsible for altered glucose metabolism in response to low magnitude changes in LPS levels.
Advisors/Committee Members: Hulver, Matthew W. (committeechair), Frisard, Madlyn I. (committee member), Davy, Kevin P. (committee member), Grange, Robert W. (committee member), Li, Liwu (committee member).
Subjects/Keywords: Endotoxin; Inflammation; Glucose Metabolism
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APA (6th Edition):
Stevens, J. R. (2014). The Effects of Low Dose Endotoxin on Glucose Homeostasis. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/64849
Chicago Manual of Style (16th Edition):
Stevens, Joseph R. “The Effects of Low Dose Endotoxin on Glucose Homeostasis.” 2014. Doctoral Dissertation, Virginia Tech. Accessed January 22, 2021.
http://hdl.handle.net/10919/64849.
MLA Handbook (7th Edition):
Stevens, Joseph R. “The Effects of Low Dose Endotoxin on Glucose Homeostasis.” 2014. Web. 22 Jan 2021.
Vancouver:
Stevens JR. The Effects of Low Dose Endotoxin on Glucose Homeostasis. [Internet] [Doctoral dissertation]. Virginia Tech; 2014. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10919/64849.
Council of Science Editors:
Stevens JR. The Effects of Low Dose Endotoxin on Glucose Homeostasis. [Doctoral Dissertation]. Virginia Tech; 2014. Available from: http://hdl.handle.net/10919/64849
Virginia Tech
11.
Frellstedt, Linda.
Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro.
Degree: MS, Biomedical and Veterinary Sciences, 2010, Virginia Tech
URL: http://hdl.handle.net/10919/44307
► Endotoxemia is responsible for severe illness in horses. Individuals can become unresponsive to the endotoxin molecule after an initial exposure; this phenomenon has been called…
(more)
▼ Endotoxemia is responsible for severe illness in horses. Individuals can become unresponsive to the
endotoxin molecule after an initial exposure; this phenomenon has been called developing a state of â
endotoxin toleranceâ (ET). ET has been induced in horses in vivo; however, cytokine expression associated with ET has not been investigated. The purpose of this study was to develop and validate a method for inducing ET in equine peripheral blood mononuclear cells (PBMCs) in vitro, and to describe the cytokine profile which is associated with the ET.
Blood was collected from 6 healthy horses and PBMCs were isolated. ET was induced by culturing cells with three concentrations of
endotoxin given to induce ET, and evaluated after a second dose of
endotoxin given to challenge the cells. The relative mRNA expression of IL-10 and IL-12 was measured by use of quantitative PCR.
ET was induced in all cells (n=6) exposed to the 2-step
endotoxin challenge. In PBMCs treated with 1.0 ng/ml of
endotoxin followed by challenge with 10 ng/ml of
endotoxin, the relative mRNA expression of IL-10 in tolerized cells was not different from positive control cells. In contrast, the relative mRNA expression of IL-12 in tolerized cells was decreased by 15-fold after the second
endotoxin challenge compared with positive control cells.
This experiment demonstrated a reliable method for the ex vivo induction of ET in equine PBMCs. A marked suppression of IL-12 production is associated with ET. The production of IL-10 was not altered in ET in our model.
Advisors/Committee Members: Furr, Martin O. (committeechair), McKenzie, Harold C. III (committee member), Barrett, Jennifer G. (committee member).
Subjects/Keywords: Endotoxin; endotoxin tolerance; interleukin-12; interleukin-10; equine
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APA ·
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APA (6th Edition):
Frellstedt, L. (2010). Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/44307
Chicago Manual of Style (16th Edition):
Frellstedt, Linda. “Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro.” 2010. Masters Thesis, Virginia Tech. Accessed January 22, 2021.
http://hdl.handle.net/10919/44307.
MLA Handbook (7th Edition):
Frellstedt, Linda. “Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro.” 2010. Web. 22 Jan 2021.
Vancouver:
Frellstedt L. Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro. [Internet] [Masters thesis]. Virginia Tech; 2010. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10919/44307.
Council of Science Editors:
Frellstedt L. Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro. [Masters Thesis]. Virginia Tech; 2010. Available from: http://hdl.handle.net/10919/44307
University of Zambia
12.
Choongo, Mutandalike.
Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
.
Degree: 2011, University of Zambia
URL: http://hdl.handle.net/123456789/884
► A study was conducted to investigate the potential of African Sausage Tree Fruit Pulp (ASTFP) in modulating the effects of E.coli endotoxin in grower pigs.…
(more)
▼ A study was conducted to investigate the potential of African Sausage Tree Fruit Pulp (ASTFP) in modulating the effects of E.coli endotoxin in grower pigs. Twenty weanling pigs from five litters/dams were randomly assigned to two dietary treatments consisting of a basic diet with 0 or 2% ASTFP. The basic diet was a farm made feed formulation fed to weanling pigs. After 14 days on basic diet, the study pigs were fed experimental diets for 42 days. The diets were fed to the study animals in the morning at 8 hours daily. On Day 42, pigs were injected with soy broth or 20 µg/kg body weight endotoxin (Lipopolysaccharide from E coli stereotype 057:B5). Body weights were monitored, rectal temperatures measured and blood samples collected before injection (0 hour) and at 2, 4, 8 and 24 hours post injection (PI). Blood samples were analysed for Urea nitrogen, Total protein, Albumin, Glucose, Cholesterol and white blood cell counts.
Body weights and growth rate were not affected by diet. The pigs fed the diet containing 2% ASTFP had higher urea nitrogen, albumin, glucose and white blood cell counts than pigs fed 0% ASTFP (P ≤ 0.05). The concentration of total protein and cholesterol were the same in both 0 and 2% ASTFP. Endotoxin challenge increased body temperature (P ≤ 0.01) and white blood cells (P ≤ 0.01). The results of this study indicate that the feed supplement of ASTFP at 2% in diets of grower pigs was beneficial in protecting pigs against the infection induced by E.coli endotoxin.
Subjects/Keywords: African Sausage Tree Fruit;
Pulp Production;
E.Coli Endotoxin
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APA (6th Edition):
Choongo, M. (2011). Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
. (Thesis). University of Zambia. Retrieved from http://hdl.handle.net/123456789/884
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Choongo, Mutandalike. “Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
.” 2011. Thesis, University of Zambia. Accessed January 22, 2021.
http://hdl.handle.net/123456789/884.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Choongo, Mutandalike. “Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
.” 2011. Web. 22 Jan 2021.
Vancouver:
Choongo M. Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
. [Internet] [Thesis]. University of Zambia; 2011. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/123456789/884.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Choongo M. Effects of African Sausage Tree Fruit Pulp on Production and Response to E-COLI Endotoxin in Grower Pigs
. [Thesis]. University of Zambia; 2011. Available from: http://hdl.handle.net/123456789/884
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Ruhr Universität Bochum
13.
Punsmann, Stefanie.
Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität.
Degree: 2014, Ruhr Universität Bochum
URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-40890
► Bioaerosole sind eine komplexe Mischung in der Luft befindlicher Partikel. Durch die anhaltende Exposition gegenüber Bioaerosolen aus Räumen mit Feuchteschäden können für die Bewohner negative…
(more)
▼ Bioaerosole sind eine komplexe Mischung in der Luft
befindlicher Partikel. Durch die anhaltende Exposition gegenüber
Bioaerosolen aus Räumen mit Feuchteschäden können für die Bewohner
negative gesundheitliche Auswirkungen entstehen. Um die Wirkung
dieser Bestandteile auf das menschliche Immunsystem
charakterisieren zu können, ist eine Betrachtung der
immunologischen Antwort auf diese Substanzen notwendig. Als
Modellsystem für die Untersuchung mikrobieller Bestandteile von
Bioaerosolen und ihrer Wirkung im humanen Blut wurde der
Vollbluttest sowohl mit frischem Vollblut von 25 Kontrollprobanden
und 25 exponierten Probanden als auch mit kryokonserviertem
Vollblut verwendet. Die hier gezeigten Ergebnisse liefern einen
ersten Hinweis auf den Einfluss der Exposition gegenüber
Bioaerosolen aus Räumen mit Feuchteschäden auf das angeborene
Immunsystem (Mediatorenfreisetzung und
Rezeptorexpression).
Advisors/Committee Members: Chemie.
Subjects/Keywords: Schimmelpilze; Endotoxin; Toll-like-Rezeptoren;
Dectin-1; Aspergillus
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APA (6th Edition):
Punsmann, S. (2014). Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-40890
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Punsmann, Stefanie. “Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität.” 2014. Thesis, Ruhr Universität Bochum. Accessed January 22, 2021.
http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-40890.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Punsmann, Stefanie. “Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität.” 2014. Web. 22 Jan 2021.
Vancouver:
Punsmann S. Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität. [Internet] [Thesis]. Ruhr Universität Bochum; 2014. [cited 2021 Jan 22].
Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-40890.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Punsmann S. Charakterisierung von Bioaerosolen aus Räumen mit
Feuchteschäden anhand ihrer pyrogenen Aktivität. [Thesis]. Ruhr Universität Bochum; 2014. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-40890
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
14.
Houben, Rosa.
Ventilation and air hygiene parameters in horse stables.
Degree: 2008, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/30487
► To assess ventilation, air hygiene and the influence of ventilation on air hygiene in horse stables, 11 barns on 4 separate premises were studied during…
(more)
▼ To assess ventilation, air hygiene and the influence of ventilation on air hygiene in horse stables, 11 barns on 4 separate premises were studied during four consecutive weeks in January and February. One of the premises used mechanical ventilation and woodshavings bedding, all others used natural ventilation and straw bedding. In all barns, inside and outside temperature and relative humidity were monitored for a week. Ammonia, stall wind speed, smoke clearance, airborne micro-organisms (fungi, gram-negative bacteria and total bacteria) and stall air and breathing zone air dust and
endotoxin levels were measured. The aim was to find a correlation between the inside-outside temperature difference and air hygiene parameters.
In the naturally ventilated barns, inside temperature and humidity were 7.5(2.8-12.7)°C and 75(67-86)%, respectively. Ammonia ranged from <2 to 11 ppm, wind speed was <0.2 to 0.6m/s. Airborne micro-organism levels were 33561(2830-35043), 510(84-1111) and 2887(344-10809) colony forming units (CFU)/m3 for fungi, gram-negative and total bacteria, respectively in the naturally ventilated barns and 769(671-3960) and 4815(2121-6222) for fungi and total bacteria in the mechanically ventilated barns. Stall air and breathing zone air dust levels, in mg/m3, were, respectively 0.24(0.16-0.58) and 2.70(0.44-12.49) in the naturally ventilated barns, and 0.46(0.13-0.98) and 7.36(1.97-45.03) in the mechanically ventilated barns. Stall air and breathing zone air
endotoxin levels, in EU/m3, were 268(86-800) and 1653(1273-48485) naturally ventilated barns, and 233(22-504) and 1974(453-15028) in the mechanically ventilated barns. Levels of dust and
endotoxin were correlated (spearman correlation 0.884). Breathing zone levels of dust and
endotoxin were significantly higher than and not correlated to stall air levels. CFU counts of fungi were significantly lower in the mechanically ventilated barns.
No correlation between barn outside-inside temperature difference and any of the air hygiene parameters was found, most likely due to a limited number of samples and variability in used source materials.
Advisors/Committee Members: Eerdenburg, Frank van.
Subjects/Keywords: Diergeneeskunde; ventilation; horse stables; air hygiene; breathing zone; dust; endotoxin
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Manager
APA (6th Edition):
Houben, R. (2008). Ventilation and air hygiene parameters in horse stables. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/30487
Chicago Manual of Style (16th Edition):
Houben, Rosa. “Ventilation and air hygiene parameters in horse stables.” 2008. Doctoral Dissertation, Universiteit Utrecht. Accessed January 22, 2021.
http://dspace.library.uu.nl:8080/handle/1874/30487.
MLA Handbook (7th Edition):
Houben, Rosa. “Ventilation and air hygiene parameters in horse stables.” 2008. Web. 22 Jan 2021.
Vancouver:
Houben R. Ventilation and air hygiene parameters in horse stables. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2008. [cited 2021 Jan 22].
Available from: http://dspace.library.uu.nl:8080/handle/1874/30487.
Council of Science Editors:
Houben R. Ventilation and air hygiene parameters in horse stables. [Doctoral Dissertation]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/30487
15.
Erica Cristina Rocha Roier.
Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido.
Degree: 2006, Universidade Federal Rural do Rio de Janeiro
URL: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=340
► This work aimed to verify the administration of virginiamicyn in starch overload horses to control the harmful effects of this overload. This work was carried…
(more)
▼ This work aimed to verify the administration of virginiamicyn in starch overload horses to control the harmful effects of this overload. This work was carried out in Universidade Federal Rural do Rio de Janeiro and consists of experimental starch overload by administration of corn starch at 17, 6 g/kg by a nasogastric probe. It was used 4 horses with a right ventral colon canula with four treatments in a latin square 4 x 4. T1 control, without virginiamicyn administration; T2 - virginiamicyn administration at same time of corn starch; T3 virginiamicyn administration 12 hours after corn starch; T4 - virginiamicyn administration at same time of corn starch and 12 hours after it. Clinical examinations and blood samples were colleted before the assay (zero time), 12, 24, 36 and 48 hours after carbohydrate overload. Besides the clinical evaluation, the endotoxin serum levels, the specific leukocyte counts and glycemia values of horses were determinate. There werent observed changes on mucosa color and refill time on treatments that virginiamicyn were administered in overload moment. Neither glycemia, nor specific and global leukocyte counts showed significant changes. Treatments used werent able to block the increase hooves temperature and digital pulse, in different levels in the horses. In other words, preventive supply of virginiamicyn at the overload moment, showed to be able to minimize the harmful effects caused by the carbohydrate overload. Better effects seem to be promoted by the virginiamicyn administration at same time and 12 hours after overload of corn starch, suggesting that sequential administration virginiamicyn is more efficient on hindgut acidosis control.
O objetivo deste trabalho foi verificar se a administração de virginiamicina em casos de sobrecarga por carboidratos de fácil fermentação (amido) é eficiente para controlar os efeitos deletérios dessa sobrecarga e prevenir o desenvolvimento de endotoxemia. O trabalho realizado na Universidade Federal Rural do Rio de Janeiro, consistiu na indução experimental de sobrecarga por amido administrando-se 17,6 g/kg de peso vivo de amido, através de sonda nasogástrica, aos animais do experimento. Foram utilizados quatro cavalos fistulados no cólon ventral direito, num delineamento quadrado latino 4x4. Os tratamentos instituídos no estudo foram C grupo controle, sem a administração de virginiamicina, V0 fornecimento de virginiamicina no momento da indução da sobrecarga por amido, V12 fornecimento de virginiamicina 12 horas após a indução da sobrecarga, V0,12 fornecimento de virginiamicina no momento da sobrecarga e 12 horas após a mesma. Avaliações clínicas, coleta de sangue e de material colônico foram realizadas nos tempos zero, 12, 24, 36 e 48 horas após sobrecarga por amido. Nas amostras de sangue foi feita mensuração dos níveis séricos de endotoxina, glicemia, leucometrias global e específica. Nas amostras de conteúdo colônico, obtidas através da cânula, foi mensurado o pH. Os dados foram submetidos à Análise de Variância para medidas repetidas com =…
Advisors/Committee Members: Paulo de Tarso Landgraf Botteon, Fernando Queiroz Almeida.
Subjects/Keywords: virginiamicyn; terapêutica; cólica; antibiótico ionóforo.; MEDICINA VETERINARIA; equine; endotoxin
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APA (6th Edition):
Roier, E. C. R. (2006). Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido. (Thesis). Universidade Federal Rural do Rio de Janeiro. Retrieved from http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=340
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Roier, Erica Cristina Rocha. “Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido.” 2006. Thesis, Universidade Federal Rural do Rio de Janeiro. Accessed January 22, 2021.
http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=340.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Roier, Erica Cristina Rocha. “Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido.” 2006. Web. 22 Jan 2021.
Vancouver:
Roier ECR. Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido. [Internet] [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2006. [cited 2021 Jan 22].
Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=340.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Roier ECR. Prevenção da endotoxemia pela administração de virginiamicina em eqüinos submetidos à sobrecarga por amido. [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2006. Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=340
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
16.
Freitas, Amanda de Souza.
Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo.
Degree: Mestrado, Saúde na Comunidade, 2014, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/17/17139/tde-15052014-143801/
;
► Em países industrializados, as doenças pulmonares são as que mais se destacam quando o assunto é doença ocupacional. Entre os técnicos, cuidadores de animais, médicos…
(more)
▼ Em países industrializados, as doenças pulmonares são as que mais se destacam quando o assunto é doença ocupacional. Entre os técnicos, cuidadores de animais, médicos e cientistas, as doenças respiratórias e alérgicas a animais de laboratório representam a principal doença ocupacional. Entre os agentes presentes na sujeira orgânica, as endotoxinas são as mais relacionadas às respostas inflamatórias e causadoras de uma série de doenças respiratórias. As endotoxinas, componentes externos das bactérias gram-negativas, são encontradas em várias concentrações em suspensão no ar ou depositadas na poeira do chão, em materiais e equipamentos, no ambiente domiciliar, urbano, rural e em alguns estabelecimentos. É inevitável a exposição à endotoxina, no entanto, o nível de exposição das vias aéreas pode ser muito variado. Alguns estudos mostraram a correlação dos sintomas apresentados pelos trabalhadores de laboratórios ou biotérios com o nível de exposição às endotoxinas. OBJETIVOS: Avaliar a exposição às endotoxinas, presentes na poeira de laboratórios e biotérios e a sua relação com asma, rinite e atopia apresentadas pelos trabalhadores. MÉTODOS: Trata-se de um estudo transversal, realizado na Universidade de São Paulo, campus Ribeirão Preto (USP-RP) e na Universidade Estadual de Campinas (UNICAMP). Foram coletadas amostras de poeira do chão de laboratórios e biotérios que continham rato, camundongo, cobaia, coelho ou hamster; e em laboratórios e salas administrativas que não tinham contato algum com esses animais. As amostras de poeira foram analisadas e a quantidade de endotoxina foi dosada pelo método Limulus amebocyte lysate (LAL). Esta quantidade foi relacionada com variáveis clínicas dos trabalhadores destes locais (sintomas, reatividade brônquica, espirometria e testes alérgicos). RESULTADOS: Foram coletadas amostras de poeira de 145 locais de trabalho. Destes, 74 (51%) da USP-RP e 71 (49%) da UNICAMP. Noventa e dois (63%) locais de trabalho continham animais de laboratório (57 da USP-RP e 35 da UNICAMP) e 53 não os continham (17 da USP-RP e 36 da UNICAMP). Foram utilizados os dados de 751 trabalhadores, 412 formaram o grupo exposto a animais de laboratório e 339 o grupo não exposto. O grupo exposto a animais de laboratório apresentou maior quantidade de endotoxina, 55 ± 79 UE por mg de poeira, quando comparado com o grupo não exposto, 19 ± 27 UE/mg (p < 0,001 pelo teste t de Student). Quando estratificada, a quantidade de endotoxina em elevada e baixa quantidade, a alta concentração (acima de 20,4 UE/mg) de endotoxina se associou ao relato de sibilos nos últimos 12 meses. Ou seja, 27% dos trabalhores expostos a elevadas concentrações relataram sibilos (p < 0,01 pelo teste do qui-quadrado). Porém, a quantidade de endotoxina não se associou com sintomas de rinite, com atopia, com o teste de hiperreatividade brônquica positiva. CONCLUSÃO: A exposição à endotoxina apresenta efeito no sistema respiratório dos trabalhadores mesmo não tendo se associado à asma. A alta concentração de endotoxina se associou com a presença de…
Advisors/Committee Members: Vianna, Elcio dos Santos Oliveira.
Subjects/Keywords: Asma; Asthma; Doença ocupacional; Endotoxin; Endotoxina; Occupational disease; Sibilo; Wheezing
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APA (6th Edition):
Freitas, A. d. S. (2014). Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17139/tde-15052014-143801/ ;
Chicago Manual of Style (16th Edition):
Freitas, Amanda de Souza. “Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo.” 2014. Masters Thesis, University of São Paulo. Accessed January 22, 2021.
http://www.teses.usp.br/teses/disponiveis/17/17139/tde-15052014-143801/ ;.
MLA Handbook (7th Edition):
Freitas, Amanda de Souza. “Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo.” 2014. Web. 22 Jan 2021.
Vancouver:
Freitas AdS. Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2021 Jan 22].
Available from: http://www.teses.usp.br/teses/disponiveis/17/17139/tde-15052014-143801/ ;.
Council of Science Editors:
Freitas AdS. Exposição à endotoxina no ambiente de trabalho e pesquisa de associação com asma, alergia e sibilo. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/17/17139/tde-15052014-143801/ ;
17.
Archilla, Jose Ricardo de Freitas.
Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular.
Degree: PhD, Dentística, 2007, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-02012008-113553/
;
► As endotoxinas são responsáveis por importantes reações sistêmicas e locais, podendo comprometer o sucesso do tratamento endodôntico. O hidróxido de cálcio, mesmo com limitações, mostra-se…
(more)
▼ As endotoxinas são responsáveis por importantes reações sistêmicas e locais, podendo comprometer o sucesso do tratamento endodôntico. O hidróxido de cálcio, mesmo com limitações, mostra-se como opção única efetiva entre as soluções irrigadoras e as medicações intracanal na redução das endotoxinas nos túbulos dentinários. O experimento realizado avaliou a eficiência do laser de Nd:YAG frente à uma endotoxina inoculada no canal radicular pela utilização de duas cinemáticas de irradiação. Após um período de incubação, confirmada a passagem da endotoxina inoculada no canal radicular através da dentina e do cemento radicular, a irradiação foi realizada ou com cinemática oscilatória ou com cinemática helicoidal, para posterior leitura e comparação com os grupos controle. Os parâmetros utilizados na irradiação com o laser de Nd:YAG de comprimento de onda de 1064 nm foram: 100mJ, 15 Hz, 1,5 W, diâmetro do núcleo da fibra 320 ?m, fluência do pulso 124 J/cm2, largura do pulso 120 ?s e intervalo de relaxação térmica 30 s. Os dados foram comparados pelo método ANOVA, complementado pelo teste de Tukey. As concentrações de endotoxina nos espécimes dos grupos irradiados (helicoidal e oscilatório) foram similares. Além disso, elas também foram similares às dos espécimes controle negativo e significantemente menores (p=0,0271) que as do controle positivo, que não sofreram irradiação. A irradiação com laser de Nd: YAG, nas condições deste experimento é efetiva na inativação da endotoxina da dentina radicular.
The endotoxins are responsible for important systemic and local reactions that could compromise the endodontic success. The calcium hydroxide even with limitations is the only effective option among the irrigant solutions and intracanal medications on the endotoxin reduction in the dentinal tubules. This experiment evaluated the Nd:YAG laser efficacy regarding the inoculated endotoxin in the root canal with two cinematic irradiation procedures. The irradiation was accomplished using an oscillatory and helicoidal technique after an incubation period where the passage of the inoculated endotoxin inside the root canal through dentin and cement was confirmed. Following this the analysis and comparison among the groups was performed. The Nd:YAG laser (?=1064 nm) irradiation, used parameters were: 100mJ, 15 Hz, 1,5 W, core fiber diameter of 320 ?m, pulse fluency of 124 J/cm2, pulse width of 120 ?s and relaxation time interval of 30 s. The data were statistically compared by ANOVA complemented by the Tukey\'s test (p<=0,005). The endotoxin concentration at the irradiated samples, regardless the techniques applied were similar. Moreover these concentrations were also similar to those of negative controls and significantly smaller than those of positive controls that were not irratiated (p=0,0271). The Nd:YAG laser irradiation under this experiment conditions was effective on the root canal endotoxin inactivation.
Advisors/Committee Members: Marques, Marcia Martins.
Subjects/Keywords: Dentin; Dentina; Endotoxin; Endotoxina; Laser de Nd:YAG; Nd:YAG laser
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APA ·
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MLA ·
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CSE |
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Manager
APA (6th Edition):
Archilla, J. R. d. F. (2007). Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/23/23134/tde-02012008-113553/ ;
Chicago Manual of Style (16th Edition):
Archilla, Jose Ricardo de Freitas. “Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021.
http://www.teses.usp.br/teses/disponiveis/23/23134/tde-02012008-113553/ ;.
MLA Handbook (7th Edition):
Archilla, Jose Ricardo de Freitas. “Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular.” 2007. Web. 22 Jan 2021.
Vancouver:
Archilla JRdF. Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 22].
Available from: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-02012008-113553/ ;.
Council of Science Editors:
Archilla JRdF. Ação do laser de Nd:YAG intracanal sobre endotoxinas na dentina radicular. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/23/23134/tde-02012008-113553/ ;
18.
Negherbon, Jesse Paul.
WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER.
Degree: 2015, Johns Hopkins University
URL: http://jhir.library.jhu.edu/handle/1774.2/37987
► The respiratory system is continuously exposed to particulate matter (PM) through the inhalation of polluted air. The composition of this PM includes an array of…
(more)
▼ The respiratory system is continuously exposed to particulate matter (PM) through the inhalation of polluted air. The composition of this PM includes an array of hazardous constituents derived from anthropogenic (e.g. – combustion) and biological sources (biogenic). A fraction of these biogenic agents are non-infectious microbial components that are often capable of engaging innate immune defense mechanisms and stimulating the release of conserved inflammatory mediators. Thus, this biogenic fraction of PM carries a unique capacity to provoke adverse respiratory inflammation in the absence of an infectious challenge or overt physical injury. Accordingly, an increasing epidemiologic evidence-base associates exposure to biogenic PM with the development (and severity) of non-infectious respiratory disorders, such as chronic bronchitis and obstructive lung disease. However, the integrative methods necessary to evaluate the pro-inflammatory potential of the full matrix of
biogenic PM are absent from customary suite of exposure assessment tools. Instead, conventional practice relies on the measurement of individual classes of agents (e.g. – endotoxins) as proxies of pro-inflammatory hazard. This strategy is partly limited by the sheer diversity of stimuli in the environment. The research presented in this dissertation investigates the pro-inflammatory immune responses to PM exposures using novel human whole blood assay (WBA) methods. The human whole blood system carries an extensive array of innate immune defense mechanisms, displays exquisite sensitivity to biologically relevant stimuli, and is amenable to ex vivo stimulation with complex mixtures of agents. This document comprehensively reviews the applicability of the WBA as an exposure assessment tool, and presents two studies in which pro-inflammatory signals of PM were assessed via WBA. The first study used cryopreserved human whole blood to investigate the pro-inflammatory potential of
respirable, thoracic, and inhalable PM from broiler poultry housing, where PM is heavily burdened with microbial components. The second study used fresh whole blood from asthmatic and non-asthmatic Peruvian children to evaluate innate immune responses to traffic PM, which has been associated the exacerbation of asthma symptoms. Taken together, this body of research demonstrates the potential of the human WBA to detect hazardous biogenic components in PM, and characterize individual immunologic responses to these complex stimuli.
Advisors/Committee Members: Scott, Alan L (advisor).
Subjects/Keywords: Endotoxin;
Particulate Matter;
Whole Blood Assay;
Asthma;
Exposure Assessment
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Export
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APA (6th Edition):
Negherbon, J. P. (2015). WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Negherbon, Jesse Paul. “WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER.” 2015. Thesis, Johns Hopkins University. Accessed January 22, 2021.
http://jhir.library.jhu.edu/handle/1774.2/37987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Negherbon, Jesse Paul. “WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER.” 2015. Web. 22 Jan 2021.
Vancouver:
Negherbon JP. WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER. [Internet] [Thesis]. Johns Hopkins University; 2015. [cited 2021 Jan 22].
Available from: http://jhir.library.jhu.edu/handle/1774.2/37987.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Negherbon JP. WHOLE BLOOD ASSAY ANALYSIS OF IMMUNOSTIMULATORY CONSTITUENTS IN ENVIRONMENTAL PARTICULATE MATTER. [Thesis]. Johns Hopkins University; 2015. Available from: http://jhir.library.jhu.edu/handle/1774.2/37987
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Saskatchewan
19.
Duke, Tanya.
Pulmonary intravascular macrophages in the rabbit.
Degree: 2010, University of Saskatchewan
URL: http://hdl.handle.net/10388/etd-02092010-141034
► Pulmonary intravascular macrophages (PIMs) promote lung inflammation and are found in ruminants, horses, pigs, cats, and dolphins, but not in primates, rats and mice. Rabbits…
(more)
▼ Pulmonary intravascular macrophages (PIMs) promote lung inflammation and are found in ruminants, horses, pigs, cats, and dolphins, but not in primates, rats and mice. Rabbits are used to study mechanisms of lung inflammation in humans, but disagreement exists whether rabbits have PIMs. This study examined rabbits for PIMs, and their influence on
endotoxin-induced lung inflammation.
Rabbits were treated with gadolinium chloride (10 mg/kg intravenous: Group GC, n=6) to produce apoptosis in PIMs, or with saline (Group SAL, n=6). Rabbits were euthanized 48 hours later. Light microscopic examination of epoxy-embedded rabbit lung sections revealed mononuclear phagocytes in alveolar septa. Transmission electron microscopy confirmed PIMs with lysosomes and close attachment to capillary endothelium. Light microscopic immuno-cytochemistry using rabbit anti-macrophage antibody (RAM-11) showed staining of septal and alveolar macrophages. There was no difference in number of RAM-11 positive septal cells between SAL and GC rabbits (P=0.2).
Rabbits were administered intravenous E.coli 0127:B8
endotoxin (100 ƒÝg/kg) 48 hours after GC (GC-LPS; n=5) or SAL treatment (SAL-LPS; n=6), and euthanized 24 hours later. Rabbits in both LPS treated groups were hypocalcaemic and exhibited compensated metabolic acidosis compared to SAL rabbits. Four rabbits died in the SAL-LPS group within 24 hours of the
endotoxin treatment and were replaced. None died in the GC-LPS group (Chi-square comparison for survival P=0.063). Greater numbers of septal heterophils were found in groups SAL-LPS and GC-LPS compared to SAL and GC. TNFƒÑ protein in serum, and IL-1ƒÒ and IL-6 mRNA in lung tissues were increased in SAL-LPS compared to SAL and GC rabbits. Lung tissues from SAL-LPS rabbits but not in GC-LPS showed moderate inflammation, but lung wet/dry ratios were not different. Lung tissue TNFƒÑ, IL-1ƒÒƒnand IL-6 mRNA, myeloperoxidase activity, and serum TNFƒÑ were reduced in GC-LPS animals compared to SAL-LPS. Immuno-electron microscopy revealed TNFƒÑ in PIMs in normal and LPS-treated rabbits. Lung and liver tissue TNFƒÑ, IL-8 and MCP-1 protein concentrations were not different between groups. GC did not appear to reduce liver inflammation. These data show that rabbits have low numbers of PIMs. GC treatment induced apoptosis in PIMs and reduced
endotoxin-induced lung inflammation and mortality.
Advisors/Committee Members: Singh, Baljit, Townsend, Hugh, Hiebert, Linda, Simko, Elemir.
Subjects/Keywords: Endotoxin; Intravascular macrophage; Rabbit
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APA (6th Edition):
Duke, T. (2010). Pulmonary intravascular macrophages in the rabbit. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-02092010-141034
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Duke, Tanya. “Pulmonary intravascular macrophages in the rabbit.” 2010. Thesis, University of Saskatchewan. Accessed January 22, 2021.
http://hdl.handle.net/10388/etd-02092010-141034.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Duke, Tanya. “Pulmonary intravascular macrophages in the rabbit.” 2010. Web. 22 Jan 2021.
Vancouver:
Duke T. Pulmonary intravascular macrophages in the rabbit. [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10388/etd-02092010-141034.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Duke T. Pulmonary intravascular macrophages in the rabbit. [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-02092010-141034
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Vilnius University
20.
Kaluinaitė,
Vilma.
IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui.
Degree: Master, 2014, Vilnius University
URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20140627_170517-74178
;
► Onkologinėmis ligomis suserga vis daugiau žmonių tiek Lietuvoje, tiek pasaulyje. Todėl svarbu išsiaiškinti veiksnius, kurie gali pagerinti ankstyvą diagnostiką, gydymą. Tyrimo tikslas: ištirti, ar IgM…
(more)
▼ Onkologinėmis ligomis suserga vis daugiau
žmonių tiek Lietuvoje, tiek pasaulyje. Todėl svarbu išsiaiškinti
veiksnius, kurie gali pagerinti ankstyvą diagnostiką, gydymą.
Tyrimo tikslas: ištirti, ar IgM klasės specifiniai natūralūs
antikūniai endotoksinai sąlygoja ilgesnį asmenų, sergančių gerklų
ir ryklės vėžiu, išgyvenamumą Tyrimo uždaviniai: 1) Nustatyti, ar
IgMNAE kiekis kraujo serume turi įtakos sergančiųjų gerklų ir
ryklės vėžiu išgyvenamumui. 2) Įvertinti vėžio išplitimo stadijos
(FIGO) ir IgMNAE kiekio kraujyje įtaką išgyvenamumui3)Ligos
išplitimo stadijos (TNM) ir IgMNAE kiekio kraujyje įtaką
išgyvenamumui. 4) Ląstelinio imuniteto ir IgMNAE kiekio kraujyje
įtaką išgyvenamumui. 5) Iššorinių vėžio rizikos veiksnių (alkoholio
ir rūkymo) ir IgMNAE kiekio įtaka išgyvenamumui. Metodai: naudotasi
Navikų imunologinės laboratorijos 1988 – 1990 m. darbo žurnalais,
Onkologijos institute esančiomis ambulatorinių kortelių ir vėžio
registro duomenimis. Rinkti duomenys apie 78 vyrus, sergančius
gerklų ir ryklės vėžiu. Duomenų analizė atlikta naudojantis SPSS
17.0 versijos statistiniu duomenų analizės paketu. Skirtumui tarp
grupių įvertinti taikytas (Chi-kvadrato) metodas, Kruscal-Wallis H
testas. Visoms hipotezėms tikrinti taikytas reikšmingumo lygmuo
0,05. Išgyvenamumo analizė atlikta naudojant Kaplan - Meier metodą.
Rezultatai: Asmenims, kurie nerūko ar surūko nedidelį cigarečių
kiekį per dieną didesnė galimybė, kad susidarys specifiniai IgMNAE
p<0,05. Piktnaudžiaujantiems... [toliau žr. visą
tekstą]
Cancer develops more and more people in
Lithuania and the world. Therefore important to find out factors
that may improve early diagnosis, treatment. The aim of research:
To investigate, does the special natural IgM class antibodies to
endotoxins leads longer people suffering from larynx and pharynx
cancer survival The task of (the) research: 1. to establish, does
IgMNAE content in blood affect survival of patients with larynx and
pharynx cancer. 2. To assess the spread of cancer stage (FIGO) and
IgMNAE content in blood influence to survival. 3. To investigate
the of the disease stage (TNM) and IgMNAE content in blood
influence to survival. 4.Cellular immunity and IgMNAE in blood
influence to survival. 5. Investigate external cancer risk factors
(smoking and alcohol) and IgMNAE content in blood influence to
survival Methods: Was used tumor immunological laboratories
1988-1990 routine work “Cancer risk and oncological patients
immunological researches” magazines, Vilnius University Oncology
institute data of existing outpatients and cancer registry,
following about: 78 trial men with larynx and pharynx cancer. Data
analysis was performed using statistical package for data analysis
SPSS version 17.0. To evaluate the difference between the groups
used (chi-square) method), Kruscal-Wallis H test with values of p
< 0,05 being defined as significant. Survival analysis was
performed using the Kaplan-Meier method. Results: For patients with
the blood of survival time median equal to ... [to full
text]
Advisors/Committee Members: Kuzmickienė, Irena (Master's thesis supervisor).
Subjects/Keywords: IgMNAE; Endotoksinas; Vėžys;
Išgyvenamumas/IgMNAE; Endotoxin; Cancer; Survival
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APA (6th Edition):
Kaluinaitė,
Vilma. (2014). IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui. (Masters Thesis). Vilnius University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20140627_170517-74178 ;
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Chicago Manual of Style (16th Edition):
Kaluinaitė,
Vilma. “IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui.” 2014. Masters Thesis, Vilnius University. Accessed January 22, 2021.
http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20140627_170517-74178 ;.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
MLA Handbook (7th Edition):
Kaluinaitė,
Vilma. “IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui.” 2014. Web. 22 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Vancouver:
Kaluinaitė,
Vilma. IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui. [Internet] [Masters thesis]. Vilnius University; 2014. [cited 2021 Jan 22].
Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20140627_170517-74178 ;.
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Council of Science Editors:
Kaluinaitė,
Vilma. IgM klasės specifinių natūralių endotoksinui
antikūnų poveikis sergančiųju gerklų ir ryklės vėžiu,
išgyvenamumui. [Masters Thesis]. Vilnius University; 2014. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20140627_170517-74178 ;
Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
University of Nairobi
21.
Ogoyi, D.O.
Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
.
Degree: 2010, University of Nairobi
URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/9789
► Objective: Three Bacillus thuringiensis isolates designated 1M, K10-2 and V24-M with varying levels of toxicity to Chilo partellus (spotted stalk borer) were characterized to establish…
(more)
▼ Objective: Three Bacillus thuringiensis isolates designated 1M, K10-2 and V24-M with varying levels of
toxicity to Chilo partellus (spotted stalk borer) were characterized to establish the basis for the differential
toxicity and to identify any unique properties that may be used to screen other isolates.
Methodology and results: Bt isolate colonies were picked from plates and inoculated into LB medium and
growth rate estimated by measurement of cell density at absorbance 600nm. Secreted proteins was
quantified and analysed through SDS-PAGE. Bt plasmid DNA was isolated and restricted with different
restriction enzymes and analysed by electrophoresis. Isolate 1M had a slightly elevated growth rate in LB
medium at the lag and exponential phases of growth than both isolates K10-2 and V24-M , but at the plateau
phase the growth rates were not significantly different for all the three isolates. All crystals were bi-pyramidal
in shape suggesting that the Bt isolates are leptidopteran specific. The major protein bands for isolate K10-2
and V24-M had molecular weights of 28, 65, and 130kDa while main protein bands in isolate 1M were of
molecular weight 28 and 65kDa. The three Bt isolates showed varying levels of plasmid DNA concentration
with isolate 1M having the highest plasmid concentration suggesting greater plasmid copy number. Upon
restriction digestion of the isolates plasmid DNA with EcoRI, BamHI and HindIII, one particular unique band
of 7200 bp was evident in all the digests.
Conclusion and application of findings: Bt isolate, 1M which had the highest toxicity against C.partellus in a
previous study showed a unique protein profile and higher plasmid copy number than the other isolates. The
results suggest that these properties may be used for selective identification of Bt isolates that exhibit
effective toxicity against C. partellus.
Subjects/Keywords: Bacillus thuringiensis;
δ- endotoxin;
plasmid,;
differential toxicity;
Chilo partellus
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APA (6th Edition):
Ogoyi, D. O. (2010). Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
. (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/9789
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ogoyi, D O. “Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
.” 2010. Thesis, University of Nairobi. Accessed January 22, 2021.
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/9789.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ogoyi, D O. “Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
.” 2010. Web. 22 Jan 2021.
Vancouver:
Ogoyi DO. Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
. [Internet] [Thesis]. University of Nairobi; 2010. [cited 2021 Jan 22].
Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/9789.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ogoyi DO. Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
. [Thesis]. University of Nairobi; 2010. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/9789
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
22.
Wong, Hoi Ting.
Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.
Degree: 2015, University of Toronto
URL: http://hdl.handle.net/1807/70727
► Being one of the leading causes of death globally and especially in intensive care units, sepsis is a medical condition of overwhelming inflammatory response due…
(more)
▼ Being one of the leading causes of death globally and especially in intensive care units, sepsis is a medical condition of overwhelming inflammatory response due to infection. Bacterial endotoxin, also known as lipopolysaccharide (LPS), plays a major role in the initiation of such response. Thus, removal of endotoxin from patient blood is a critical treatment to minimize mortality. However, problematic detection of LPS in blood leads to difficulties in preparing therapy targeting LPS and monitoring patient condition. In fact, there is no medicine currently available in the North Americas. Here in, a new approach to reduce endotoxin concentration in blood with the use of polymyxin B (PMB)-modified glass beads is presented. An alternative mechanism of LPS-PMB interaction as opposed to the generally accepted mechanism was presented in this manuscript. The LPS-PMB interaction is characterized and evaluated by gas chromatography and enzyme-linked immunosorbent assay (ELISA).
M.Sc.
Advisors/Committee Members: Thompson, Michael, Chemistry.
Subjects/Keywords: Bead Chemistry; Endotoxemia; Endotoxin; Lipopolysaccharide (LPS); Polymyxin B (PMB); Sepsis; 0485
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APA (6th Edition):
Wong, H. T. (2015). Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70727
Chicago Manual of Style (16th Edition):
Wong, Hoi Ting. “Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.” 2015. Masters Thesis, University of Toronto. Accessed January 22, 2021.
http://hdl.handle.net/1807/70727.
MLA Handbook (7th Edition):
Wong, Hoi Ting. “Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.” 2015. Web. 22 Jan 2021.
Vancouver:
Wong HT. Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1807/70727.
Council of Science Editors:
Wong HT. Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70727
University of Guelph
23.
Fisher, Rebecca Elizabeth.
Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming.
Degree: PhD, Department of Animal and Poultry Science, 2013, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7689
► Adverse uterine environments caused by stressors such as an infection during pregnancy can alter the programming of the fetus, increasing the risk of adulthood inflammatory…
(more)
▼ Adverse uterine environments caused by stressors such as an infection during pregnancy can alter the programming of the fetus, increasing the risk of adulthood inflammatory disease. Supplementation with fishmeal (FM; rich in anti-inflammatory omega-3 polyunsaturated fatty acids; n-3 PUFAs) during pregnancy may help to reduce this risk. To test this hypothesis, 53 ewes were allocated to either a diet supplemented with FM or soybean meal (SM; rich in n-6 PUFAs), and on gestation day 135 (gd135) half the ewes from each dietary treatment were challenged with either 1.2 μg/kg lipopolysaccharide (LPS) to simulate a bacterial infection, or saline as a control (CON). The cortisol response of the offspring was assessed at weaning ± ACTH challenge, and during adulthood by LPS
endotoxin challenge. Immune responsiveness of the adult offspring was assessed with a skin hypersensitivity test using antigens, ovalbumin (OVA) and candida albicans (CAA), and by measuring the OVA-specific serum antibody response. The FM+LPS treatment lambs had the greatest cortisol response compared to all other treatment groups during both weaning and
endotoxin challenge. In contrast, the SM+LPS lambs had a greater cortisol response compared to the SM+CON treatment group during weaning + ACTH challenge. The SM+LPS offspring had the greatest hypersensitivity response to both OVA and CAA as well as the greatest serum IgG response to OVA compared to all other treatment groups. Lastly, the expression of hippocampal glucocorticoid and mineralcorticoid receptor genes (GR and MR) were shown to be different, with FM+LPS females having greater GR expression compared to SM+LPS females, while the reverse was true for MR. These data suggest that FM may help protect the offspring from immune system and HPAA programming during maternal infection. Future studies need to focus on the mechanisms behind these alterations.
Advisors/Committee Members: Karrow, Niel (advisor).
Subjects/Keywords: Fetal Programming; Hypothalamic-Pituitary-Adrenal Axis; Ovine; Fishmeal; Endotoxin
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MLA ·
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CSE |
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fisher, R. E. (2013). Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7689
Chicago Manual of Style (16th Edition):
Fisher, Rebecca Elizabeth. “Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming.” 2013. Doctoral Dissertation, University of Guelph. Accessed January 22, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7689.
MLA Handbook (7th Edition):
Fisher, Rebecca Elizabeth. “Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming.” 2013. Web. 22 Jan 2021.
Vancouver:
Fisher RE. Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming. [Internet] [Doctoral dissertation]. University of Guelph; 2013. [cited 2021 Jan 22].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7689.
Council of Science Editors:
Fisher RE. Fishmeal Supplementation During Gestation and Lactation Protects Offspring Against Maternal Simulated Infection Induced Programming. [Doctoral Dissertation]. University of Guelph; 2013. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7689
24.
DAVID NG SHEN WEN.
Role of H2S in Acute and Chronic inflammation.
Degree: 2014, National University of Singapore
URL: http://scholarbank.nus.edu.sg/handle/10635/119480
Subjects/Keywords: H2S; inflammation; atherosclerosis; endotoxin tolerance
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Manager
APA (6th Edition):
WEN, D. N. S. (2014). Role of H2S in Acute and Chronic inflammation. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/119480
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
WEN, DAVID NG SHEN. “Role of H2S in Acute and Chronic inflammation.” 2014. Thesis, National University of Singapore. Accessed January 22, 2021.
http://scholarbank.nus.edu.sg/handle/10635/119480.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
WEN, DAVID NG SHEN. “Role of H2S in Acute and Chronic inflammation.” 2014. Web. 22 Jan 2021.
Vancouver:
WEN DNS. Role of H2S in Acute and Chronic inflammation. [Internet] [Thesis]. National University of Singapore; 2014. [cited 2021 Jan 22].
Available from: http://scholarbank.nus.edu.sg/handle/10635/119480.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
WEN DNS. Role of H2S in Acute and Chronic inflammation. [Thesis]. National University of Singapore; 2014. Available from: http://scholarbank.nus.edu.sg/handle/10635/119480
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Iowa
25.
Kilburg-Basnyat, Brita Jane.
Validation of electrostatic dust collectors (EDCs) as effective passive samplers.
Degree: PhD, Human Toxicology, 2015, University of Iowa
URL: https://ir.uiowa.edu/etd/1978
► Electrostatic Dust Collectors (EDCs) are a passive sampling method that has not yet been fully validated. Our first study investigated the effect of EDC…
(more)
▼ Electrostatic Dust Collectors (EDCs) are a passive sampling method that has not yet
been fully validated. Our first study investigated the effect of EDC mailing and EDC
deployment in front of and away from heated ventilation on
endotoxin concentrations.
Endotoxin sampling efficiency of heated and unheated EDC cloths was evaluated. EDCs
express mailed cross-country yielded no significant changes in
endotoxin
concentrations when dust-only samples were compared to high quality control (QC)
spiked-EDCs (
p=0.21) and low QC spiked-EDCs (
p=0.16). EDCs were
deployed in 20 apartments with one EDC placed in front of the univent heater and
another EDC placed on a built-in bookshelf.
Endotoxin concentrations were
significantly different (
p=0.049) indicating that EDC placement impacts
endotoxin sampling. Heated and unheated EDCs were deployed for 7 days in farm homes.
There was a significant difference between
endotoxin concentrations
(
p=0.027). The electrostatic charge of 12 heated and 12 unheated EDC cloths
were significantly different (
p=0.009). These studies suggest that heating
cloths may diminish their electrostatic charge and
endotoxin sampling capabilities.
The EDC sampling time needed to achieve detectable and reproducible loading for
bioaerosols has not been systematically evaluated. In our second study, EDCs were
deployed in 15 Iowa farm homes for 7-, 14-, and 28-day sampling periods to determine
if
endotoxin and allergens could be quantified and if loading rates were uniform
(i.e. doubling from 7 to 14 days and 14 to 28 days and quadrupling from 7 to 28
days). Loadings between left and right paired EDC cloths were not significantly
different and were highly correlated for
endotoxin, total protein, and cat (
Fel
d1), dog (
Can f1) and mouse (
Mus m1) allergens
(
p<0.001). EDC
endotoxin sampling had close agreement between paired
samples (Pearson
p=0.96,
p<0.001). EDC
endotoxin loading
doubled from 7 to 14-day deployments but the loading rate decreased from 14 to 28
days of sampling with only a 1.38 fold increase. Allergen exposure assessment using
EDCs was less satisfactory.
Paired EDCs and daily Button aerosol samplers (BS) were used in our third study to
concurrently sample
endotoxin in 10 farm homes during 7 day periods in summer and
winter. Winter sampling included an optical particle counter (OPC) for particulate
size and number concentration data. OPC particulate matter (PM) data were divided
into…
Advisors/Committee Members: Thorne, Peter S. (supervisor).
Subjects/Keywords: publicabstract; bioaerosols; endotoxin; exposure assessment; house dust; passive sampling; Toxicology
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APA (6th Edition):
Kilburg-Basnyat, B. J. (2015). Validation of electrostatic dust collectors (EDCs) as effective passive samplers. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1978
Chicago Manual of Style (16th Edition):
Kilburg-Basnyat, Brita Jane. “Validation of electrostatic dust collectors (EDCs) as effective passive samplers.” 2015. Doctoral Dissertation, University of Iowa. Accessed January 22, 2021.
https://ir.uiowa.edu/etd/1978.
MLA Handbook (7th Edition):
Kilburg-Basnyat, Brita Jane. “Validation of electrostatic dust collectors (EDCs) as effective passive samplers.” 2015. Web. 22 Jan 2021.
Vancouver:
Kilburg-Basnyat BJ. Validation of electrostatic dust collectors (EDCs) as effective passive samplers. [Internet] [Doctoral dissertation]. University of Iowa; 2015. [cited 2021 Jan 22].
Available from: https://ir.uiowa.edu/etd/1978.
Council of Science Editors:
Kilburg-Basnyat BJ. Validation of electrostatic dust collectors (EDCs) as effective passive samplers. [Doctoral Dissertation]. University of Iowa; 2015. Available from: https://ir.uiowa.edu/etd/1978
University of Southern California
26.
Wang, Jun.
Common immune-related factors and risk of non-Hodgkin
lymphomy.
Degree: PhD, Epidemiology, 2012, University of Southern California
URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/115680/rec/1466
► Non-Hodgkin lymphoma (NHL) is a highly heterogeneous group of neoplasms originating from B- or T- lymphocytes, with the vast majority of B-cell origin. It is…
(more)
▼ Non-Hodgkin lymphoma (NHL) is a highly heterogeneous
group of neoplasms originating from B- or T- lymphocytes, with the
vast majority of B-cell origin. It is believed that immune
dysregulation plays an important role in the etiology of NHL.
Currently, the strongest risk factor is immune deficiency,
including primary or acquired immune deficiency, and
immunosuppressive therapy after organ transplant. However, these
conditions are rare in the general population and therefore do not
account for the majority of the cases. A remaining key question is
whether mild to moderate immune dysregulation could also contribute
to NHL risk given that immune deficiency, especially severe immune
deficiency, has been established as a strong risk factor. ❧
Extensive epidemiological studies have investigated the association
between common immune-related diseases/conditions and NHL risk.
Autoimmune or atopic diseases and infections are among those most
studied. Autoimmune rheumatic conditions have been shown to
increased NHL risk despite the debate regarding whether
immunosuppressive therapy for autoimmune disease may also
contribute to the development of NHL. Atopic diseases, on the other
hand, have been consistently reported to be inversely associated
with NHL risk in case-control studies although a disease effect
cannot be ruled out: NHL may interfere with B-cell’s ability to
produce immunoglobulin E (IgE). Surrogates for early life
infections, such as later birth order and large sibship size, have
been shown to be positively associated with NHL risk. ❧ Progress
has also been made on understanding genetic susceptibility to NHL.
Currently, the most consistent findings come from immune response
genes, with evidence from both candidate gene and genome wide
association studies. Particularly, tumor necrosis factor – alpha
(TNF-α), interleukin-10 (IL-10), and lymphotoxin alpha (LTA) are
among the best characterized and validated genes. ❧ The full
spectra of the biologic mechanisms for NHL are not understood.
However, the critical role of chronic antigenic stimulation in
lymphomagenesis has been largely appreciated. Nevertheless, the
nature of the immune dysfunction in terms of lymphomagenesis is
still puzzling, e.g. both immune suppression (severe
immunodeficiency) and stimulation (chronic B-cell activation) have
been implicated in the pathogenesis of NHL. The objective of this
dissertation was to understand how common immune-altering factors,
including environmental stimulant (e.g. household
endotoxin) and
immune-related medical history, would affect risk for NHL in the
general populations, with emphasis on atopic diseases and
infections. ❧ We did not find any association between household
endotoxin levels, measured from dust samples collected from
participants’ vacuum cleaner bags, and NHL risk in a NCI/SEER
multi-center population based case-control study. The null finding
could be due to the nature of cross-sectional measurement of
endotoxin or a single measurement which may not reflect a long term
exposure. ❧ In a case-control study…
Advisors/Committee Members: Cozen, Wendy (Committee Chair), Mack, Thomas M. (Committee Member), Stern, Mariana C. (Committee Member), Berhane, Kiros T. (Committee Member), Akbari, Omid (Committee Member).
Subjects/Keywords: non-Hodgkin lymphoma; epidemiology; etiology; immunity; endotoxin; atopy; immune-response genes
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, J. (2012). Common immune-related factors and risk of non-Hodgkin
lymphomy. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/115680/rec/1466
Chicago Manual of Style (16th Edition):
Wang, Jun. “Common immune-related factors and risk of non-Hodgkin
lymphomy.” 2012. Doctoral Dissertation, University of Southern California. Accessed January 22, 2021.
http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/115680/rec/1466.
MLA Handbook (7th Edition):
Wang, Jun. “Common immune-related factors and risk of non-Hodgkin
lymphomy.” 2012. Web. 22 Jan 2021.
Vancouver:
Wang J. Common immune-related factors and risk of non-Hodgkin
lymphomy. [Internet] [Doctoral dissertation]. University of Southern California; 2012. [cited 2021 Jan 22].
Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/115680/rec/1466.
Council of Science Editors:
Wang J. Common immune-related factors and risk of non-Hodgkin
lymphomy. [Doctoral Dissertation]. University of Southern California; 2012. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/115680/rec/1466
University of Georgia
27.
Visser, Molly Catherine.
A two part thesis.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/23270
► The first study investigates serum IgE and residential endotoxin levels from a cohort of 19 pregnant women living in Trujillo, Peru, and investigates correlations between…
(more)
▼ The first study investigates serum IgE and residential endotoxin levels from a cohort of 19 pregnant women living in Trujillo, Peru, and investigates correlations between IgE, endotoxin, demographic, and lifestyle factors in this cohort.
Geometric mean for IgE and endotoxin were 246.8 (GSD=4.3) IU/mL and 66.5 EU/mg (GSD=1.7), respectively. Residential endotoxin and serum IgE were not correlated in this cohort. Seventy four percent (14/19) of the subjects in this study had an IgE over 100
IU/mL, a level generally considered elevated. The second study investigates real-time and primarily 24-hr time-integrated levels of PM2.5 (particulate matter d 2.5 microns in diameter) inside and outside (up to 500 ft from the house) of commercial
broiler house during tunnel ventilation on a farm in northeast Georgia. None of the 24-hr PMmeasures collected when the houses where in tunnel ventilation 2.5 exceeded the U.S. EPA 24-hr National Ambient Air Quality Standard of 65 ug/m3.
Subjects/Keywords: IgE; endotoxin; Peru; broiler house; emission; particulate matter
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APA (6th Edition):
Visser, M. C. (2014). A two part thesis. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/23270
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Visser, Molly Catherine. “A two part thesis.” 2014. Thesis, University of Georgia. Accessed January 22, 2021.
http://hdl.handle.net/10724/23270.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Visser, Molly Catherine. “A two part thesis.” 2014. Web. 22 Jan 2021.
Vancouver:
Visser MC. A two part thesis. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10724/23270.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Visser MC. A two part thesis. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/23270
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
28.
Williams, Jarred Mathew.
Presence of soluble CD14 in bovine and equine milk.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/29352
► Soluble CD14 (sCD14) is a protein receptor specific for LPS, which circulates in the blood of many species, such as cows and horses. We showed…
(more)
▼ Soluble CD14 (sCD14) is a protein receptor specific for LPS, which circulates in the blood of many species, such as cows and horses. We showed in equine colostrum, and re-confirmed in bovine colostrum, a 48 kD isoform of sCD14. Colostral
sCD14 concentrations were higher than serum sCD14 levels from lactating equine and bovine dams. Concentrations of the 48 kD isoform of sCD14 were very low in serum taken from foals and calves before nursing, but very high in serum from the same neonates
24 hours after ingestion of colostrums. Also, bovine mammary epithelial cells were cultured and identified, and the 56 and 50 kD isoforms of sCD14 were detected in the lysates. These findings indicate a role for sCD14 in bovine and equine colostrum, and
neonatal immunity.
Subjects/Keywords: Colostrum; Soluble CD14; Bovine Mammary Epithelial Cells,; Endotoxin; Septicemia; Passive Immunity
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Williams, J. M. (2014). Presence of soluble CD14 in bovine and equine milk. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29352
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Williams, Jarred Mathew. “Presence of soluble CD14 in bovine and equine milk.” 2014. Thesis, University of Georgia. Accessed January 22, 2021.
http://hdl.handle.net/10724/29352.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Williams, Jarred Mathew. “Presence of soluble CD14 in bovine and equine milk.” 2014. Web. 22 Jan 2021.
Vancouver:
Williams JM. Presence of soluble CD14 in bovine and equine milk. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10724/29352.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Williams JM. Presence of soluble CD14 in bovine and equine milk. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29352
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
29.
Wong, Jonathan.
Studies of unexplained inflammation in haemodialysis and the role of endotoxin.
Degree: PhD, 2019, University of Hertfordshire
URL: http://hdl.handle.net/2299/22285
► Clinical outcomes for patients with end-stage kidney disease on haemodialysis remain poor and have similar mortality risk to some cancers. A significant proportion of haemodialysis…
(more)
▼ Clinical outcomes for patients with end-stage kidney disease on haemodialysis remain poor and have similar mortality risk to some cancers. A significant proportion of haemodialysis patients have chronic unexplained inflammation - the pathophysiology of this syndrome is unclear but is associated with poor quality of life and survival. A large number of studies have proposed that circulating endotoxin, fragments of gram negative bacterial cell wall, plays a key role in driving chronic inflammation in dialysis patients in the absence of clinical infection. However, interpretation of published literature is difficult for several reasons. Firstly, reports have mainly been cross-sectional in nature. Secondly, various different types of endotoxin detection assays have been used and reported blood endotoxin levels in clinically stable non-infected patients were exceedingly high which is incompatible with the clinical state. Additionally, nearly all studies used endotoxin detection assays that could be also be activated by (1-3)-β-D glucan, a cell wall constituent of cereals, yeast and fungi. This is a particularly important given that materials used to construct some dialyser membranes are known to leach (1-3)-β-D glucan. The doctoral research project had two central hypotheses requiring investigation. Firstly, that endotoxin and other exogenous molecules in the gut including (1-3)-β-D glucan are contributors to unexplained inflammation in patients with chronic kidney disease. Second, that their presence in blood is a result of translocation from the gut due to increased gastrointestinal permeability in the uraemic state. The clinical importance of these hypotheses is that if the relationship between presence of endotoxin and (1-3)-β-D glucan in blood of patients with chronic kidney disease and inflammation were clarified, and a positive association was confirmed, this would justify embarking on endotoxin or (1-3)-β-D glucan lowering interventional studies such as extracorporeal endotoxin adsorption therapy or exploring therapies to modify gut flora. In order to address these hypotheses systematically, the studies were designed with the following specific aims: 1. To determine the prevalence of chronic unexplained inflammation in patients receiving haemodialysis to determine the scope and extent of the clinical problem. 2. To determine the optimum endotoxin assay, in terms of accuracy and precision, for measurement of endotoxin in uraemic human blood and to limit interference of other factors in the assay such as presence of (1-3)-β-D glucan. 3. To determine the relationship of blood levels of endotoxin measured with an optimised assay and (1-3)-β-D glucan in patients with chronic kidney disease with patient symptoms and inflammation both cross-sectionally and longitudinally. 4. To determine factors influencing the presence of endotoxin and (1-3)-β-D glucan in the blood of patients requiring haemodialysis to determine whether increased gut permeability, potentially induced by intravascular volume depletion during dialysis,…
Subjects/Keywords: Haemodialysis; chronic kidney disease; inflammation; endotoxin; (1-3)-ß-D glucan
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APA (6th Edition):
Wong, J. (2019). Studies of unexplained inflammation in haemodialysis and the role of endotoxin. (Doctoral Dissertation). University of Hertfordshire. Retrieved from http://hdl.handle.net/2299/22285
Chicago Manual of Style (16th Edition):
Wong, Jonathan. “Studies of unexplained inflammation in haemodialysis and the role of endotoxin.” 2019. Doctoral Dissertation, University of Hertfordshire. Accessed January 22, 2021.
http://hdl.handle.net/2299/22285.
MLA Handbook (7th Edition):
Wong, Jonathan. “Studies of unexplained inflammation in haemodialysis and the role of endotoxin.” 2019. Web. 22 Jan 2021.
Vancouver:
Wong J. Studies of unexplained inflammation in haemodialysis and the role of endotoxin. [Internet] [Doctoral dissertation]. University of Hertfordshire; 2019. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/2299/22285.
Council of Science Editors:
Wong J. Studies of unexplained inflammation in haemodialysis and the role of endotoxin. [Doctoral Dissertation]. University of Hertfordshire; 2019. Available from: http://hdl.handle.net/2299/22285
Virginia Tech
30.
Han, Janet.
Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses.
Degree: MS, Veterinary Medical Sciences, 2008, Virginia Tech
URL: http://hdl.handle.net/10919/33902
► The objective of the study was to investigate and characterize the effects of a continuous rate infusion of dextrose or dextrose and insulin on glucose…
(more)
▼ The objective of the study was to investigate and characterize the effects of a continuous rate infusion of dextrose or dextrose and insulin on glucose and insulin dynamics in both healthy and
endotoxin-exposed horses. Administration of a low dose of
endotoxin has been used in horses to mimic the clinicopathologic changes seen in endotoxemia, including the development of an inflammatory response. Our hypothesis was that a continuous rate infusion of insulin at a rate of 0.07 IU/kg/hr would prevent the development of hyperglycemia induced by administration of dextrose in both healthy and
endotoxin-exposed horses. Nine healthy adult horses were used in the study.
In Phase 1 of the experiment, horses received a saline infusion or a dextrose infusion in a balanced crossover design. In Phase 2 of the experiment, horses received a dextrose and insulin infusion, both prior to and after receiving a low dose of
endotoxin (no LPS group and LPS group respectively) in a balanced crossover design. Blood samples were collected at regular intervals throughout both phases for measurement of plasma glucose and insulin concentrations.
Infusion of dextrose alone resulted in hyperglycemia for nearly the entire study period. Insulin concentration was also increased in comparison to the saline infusion. When comparing the dextrose treatment group to the combined dextrose and insulin treatment group (no LPS group), the insulin levels were significantly greater over time in the latter group and resulted in maintenance of euglycemia. When comparing the no LPS group to the LPS group, both the glucose and insulin concentrations were higher in the LPS group but euglycemia was still achieved. These results serve to validate the dose of insulin used in this study (0.07 IU/kg/hr) in regards to effective prevention of hyperglycemia when administered concurrently with a dextrose infusion. Hyperglycemia was prevented in both healthy and
endotoxin-exposed horses. In addition, the dose of insulin used was demonstrated to be safe, as hypoglycemia did not occur in any of the horses.
Advisors/Committee Members: McKenzie, Harold C. III (committeechair), McCutcheon, L. Jill (committee member), Geor, Raymond J. (committee member), Furr, Martin O. (committee member).
Subjects/Keywords: glucose; insulin; dextrose; endotoxin; horse
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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APA (6th Edition):
Han, J. (2008). Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/33902
Chicago Manual of Style (16th Edition):
Han, Janet. “Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses.” 2008. Masters Thesis, Virginia Tech. Accessed January 22, 2021.
http://hdl.handle.net/10919/33902.
MLA Handbook (7th Edition):
Han, Janet. “Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses.” 2008. Web. 22 Jan 2021.
Vancouver:
Han J. Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses. [Internet] [Masters thesis]. Virginia Tech; 2008. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/10919/33902.
Council of Science Editors:
Han J. Glucose and insulin dynamics associated with continuous infusion of dextrose or dextrose and insulin in healthy and endotoxin-exposed horses. [Masters Thesis]. Virginia Tech; 2008. Available from: http://hdl.handle.net/10919/33902
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Open Access Theses and Dissertations