subject:(endotoxemia)

1. SAMPAIO, Bruno. Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia .

Degree: 2012, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/11011

► Atualmente, a obesidade é considerada um importante problema de saúde pública mundial. Notavelmente, o aumento da prevalência desta síndrome está sendo associado ao surgimento de… (more)

▼ Atualmente, a obesidade é considerada um importante problema de saúde pública mundial. Notavelmente, o aumento da prevalência desta síndrome está sendo associado ao surgimento de inúmeras doenças secundárias (co-morbidades) que prejudicam a qualidade de vida e agravam o prognóstico de indivíduos e pacientes, tornando-os mais predisponentes à doenças inflamatórias e infecções. Trata-se de uma condição metabólica complexa, que influencia diversos sistemas fisiológicos, inclusive o sistema imune. O tecido adiposo, que antes era conhecido por funções de armazenamento de energia, vem sendo descrito ultimamente como importante órgão endócrino, capaz de secretar uma gama de moléculas pró e anti-inflamatórias (inclusive citocinas), as quais exercem atividade direta sobre os componentes do sistema imune. Com base nesse contexto, a presente pesquisa se propôs a avaliar o efeito e as consequências da obesidade sobre parâmetros do sangue e do sistema imune, como a funcionalidade de macrófagos alveolares, em ratas adultas submetidas à endotoxemia. Neste estudo, ratas Wistar (n=32) foram divididas em dois grupos, segundo regime dietético empregado: Grupo Dieta Padrão (DP), formado por animais que receberam dieta padrão do biotério, Labina (Purina do Brasil S/A), durante 18 semanas e Grupo Dieta Hiperlipídica (DH), composto por animais que receberam dieta palatável hiperlipídica por um período de 18 semanas (148 dias). No 147° dia, os grupos iniciais foram subdividos em endotoxêmicos (DPE e DHE) e não endotoxêmicos (DH e DP). A obtenção dos grupos endotoxêmicos foi feita com aplicação intraperitoneal de lipopolissacarídeo bacteriano (LPS) na dose 1mg/Kg de peso corporal. Após 24h, foram realizadas a obtenção de sangue periférico para contagem de leucócitos e hemácias, a retirada do lavado broncoalveolar (LBA) e a retirada da gordura abdominal para posterior pesagem. Alíquotas do LBA foram extraídas para a análise da contagem total e diferencial de leucócitos. Após, o LBA foi centrifugado para obtenção dos macrófagos alveolares (MA). Foi efetuado o estudo da atividade microbicida dos MA, através da taxa de fagocitose e da produção de óxido nítrico (ON), através da medida indireta da dosagem dos nitritos e nitratos nos cultivos celulares. Foram realizados também testes de viabilidade celular. A análise estatística foi efetuada com os testes MANOVA e ANOVA. Os resultados revelam que o consumo de dieta hiperlipídica não gerou aumento no ganho ponderal, todavía promoveu incremento da quantidade de gordura visceral. A obesidade, gerada pela indução da dieta hiperlipídica, acarretou um aumentou na quantidade de hemácias e neutrófilos do sangue periférico e na quantidade de neutrófilos e linfócitos no local da infecção. Surpreendetemente, as atividades microbicidas dos MA de ratas obesas permaneceram semelhantes e intactas, mesmo na presença da endotoxemia. Advisors/Committee Members: ANDRADE, Maria do Amparo (advisor), CASTRO, Célia Maria Machado Barbosa de (advisor).

Subjects/Keywords: Obesidade; Imunidade; Macrófagos alveolares; Endotoxemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SAMPAIO, B. (2012). Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/11011

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SAMPAIO, Bruno. “Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia .” 2012. Thesis, Universidade Federal de Pernambuco. Accessed January 22, 2021. http://repositorio.ufpe.br/handle/123456789/11011.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SAMPAIO, Bruno. “Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia .” 2012. Web. 22 Jan 2021.

Vancouver:

SAMPAIO B. Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2012. [cited 2021 Jan 22]. Available from: http://repositorio.ufpe.br/handle/123456789/11011.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SAMPAIO B. Efeitos da obesidade sobre parâmetros imunológicos e hematológicos de ratas submetidas à endotoxemia . [Thesis]. Universidade Federal de Pernambuco; 2012. Available from: http://repositorio.ufpe.br/handle/123456789/11011

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kashmir

2. Bashir, Asma. Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -.

Degree: Bio Technology, 2012, University of Kashmir

URL: http://shodhganga.inflibnet.ac.in/handle/10603/9537

►

Bacterial endotoxin stimulates macrophages / monocytes to release various cytokines early (e.g., TNF-and#945;, IL-1and#946;, and IFN-and#947;) and late (HMGB-1) which then mediate sepsis (or endotoxemia).… (more)

Subjects/Keywords: Bio Technology; cellular milieu; endotoxemia

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APA (6^th Edition):

Bashir, A. (2012). Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -. (Thesis). University of Kashmir. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/9537

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bashir, Asma. “Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -.” 2012. Thesis, University of Kashmir. Accessed January 22, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/9537.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bashir, Asma. “Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -.” 2012. Web. 22 Jan 2021.

Vancouver:

Bashir A. Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -. [Internet] [Thesis]. University of Kashmir; 2012. [cited 2021 Jan 22]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9537.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bashir A. Targeting HMGB1 in cellular milieu and elucidating its effect in mice model of endotoxemia; -. [Thesis]. University of Kashmir; 2012. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/9537

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Estado do Rio de Janeiro

3. Marcos Lopes de Miranda. Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters.

Degree: Master, 2013, Universidade do Estado do Rio de Janeiro

URL: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9492 ;

►

Pela sua alta incidência, morbidade, mortalidade e custos ao sistema de saúde, a sepse se destaca entre as diversas indicações de internação em unidade de… (more)

▼

Pela sua alta incidência, morbidade, mortalidade e custos ao sistema de saúde, a sepse se destaca entre as diversas indicações de internação em unidade de terapia intensiva (UTI). A disfunção da microcirculação tem papel central na gênese e manutenção da síndrome séptica, sendo um marco fisiopatológico desta síndrome. Pacientes críticos invariavelmente estão ansiosos, agitados, confusos, desconfortáveis e/ou com dor. Neste contexto, drogas sedativas são amplamente utilizadas na medicina intensiva. A dexmedetomidina, um agonista potente e altamente seletivo dos receptores alfa-2 adrenérgicos, vem conquistando espaço como o sedativo de escolha nas UTIs por seus efeitos de sedação consciente, redução da duração e incidência de delirium e preservação da ventilação espontânea. Apesar destas possíveis vantagens, a indicação de uso da dexmedetomidina na síndrome séptica ainda carece de conhecimentos sobre seus efeitos na microcirculação e perfusão orgânica. Com o intuito de caracterizar os efeitos microcirculatórios da dexmedetomidina em um modelo murino de endotoxemia que permite estudos in vivo da inflamação e disfunção da perfusão microvascular, hamsters Sírios dourados submetidos à endotoxemia induzida por administração intravenosa de lipopolissacarídeo de Escherichia coli (LPS, 1,0 mg.kg-1) foram sedados com dexmedetomidina (5,0 μg.kg.h-1). A microscopia intravital da preparação experimental (câmara dorsal) permitiu a realização de uma análise quantitativa das variáveis microvasculares e do rolamento e adesão de leucócitos à parede venular. Também foram analisados os parâmetros macro-hemodinâmicos e gasométricos (arterial e venoso portal), as concentrações de lactato arterial e venoso portal, a água pulmonar total e a sobrevivência do animal. Animais não-endotoxêmicos e/ou tratados com solução salina a 0,9% serviram como controles neste experimento. O LPS aumentou o rolamento e a adesão de leucócitos à parede venular, diminuiu a densidade capilar funcional e a velocidade das hemácias nos capilares e induziu acidose metabólica. O tratamento com dexmedetomidina atenuou significativamente estas respostas patológicas (p < 0,05). A frequência de pulso dos animais foi significativamente reduzida pela droga (p < 0,05). Outros resultados não foram tão expressivos (estatisticamente ou clinicamente). Estes resultados indicam que a utilização de dexmedetomidina produz um efeito protetor sobre a microcirculação da câmara dorsal de hamsters endotoxêmicos. Efeitos anti-inflamatórios da dexmedetomidina sobre os leucócitos e o endotélio poderiam melhorar a perfusão capilar e representar o mecanismo in vivo de ação da droga na microcirculação.

Due to its high incidence, morbidity, mortality and costs to the healthcare system, sepsis stands out among the many indications for intensive care unit (ICU) admission. The microcirculatory dysfunction plays a central role in the genesis and maintenance of the septic syndrome, being a pathophysiologic milestone in this syndrome. Critically ill patients are invariably anxious, agitated,…

Advisors/Committee Members: Nivaldo Ribeiro Villela, Eliete Bouskela, Hugo Caire de Castro Faria Neto.

Subjects/Keywords: Sedação; Dexmedetomidina; Endotoxemia; Microcirculação; Sedation; Dexmedetomidine; Endotoxemia; Microcirculation; MEDICINA; Dexmedetomidina; Sedação Consciente; Endotoxemia Teses; Microcirculação Teses; Síndrome de Resposta Inflamatória Sistêmica

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miranda, M. L. d. (2013). Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters. (Masters Thesis). Universidade do Estado do Rio de Janeiro. Retrieved from http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9492 ;

Chicago Manual of Style (16^th Edition):

Miranda, Marcos Lopes de. “Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters.” 2013. Masters Thesis, Universidade do Estado do Rio de Janeiro. Accessed January 22, 2021. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9492 ;.

MLA Handbook (7^th Edition):

Miranda, Marcos Lopes de. “Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters.” 2013. Web. 22 Jan 2021.

Vancouver:

Miranda MLd. Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters. [Internet] [Masters thesis]. Universidade do Estado do Rio de Janeiro; 2013. [cited 2021 Jan 22]. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9492 ;.

Council of Science Editors:

Miranda MLd. Avaliação das alterações macro-hemodinâmicas, microcirculatórias, gasométricas, metabólicas e inflamatórias secundárias à sedação com dexmedetomidina em um modelo experimental de endotoxemia em hamsters. [Masters Thesis]. Universidade do Estado do Rio de Janeiro; 2013. Available from: http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9492 ;

NSYSU

4. Li, Chuei-Shiun. The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia.

Degree: Master, Biological Sciences, 2011, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210111-225538

► Autophagy is an important cellular process in maintenance of protein homeostasis. Emerging evidence indicates differential roles of autophagy in cellular function under different pathophysiologic conditions.… (more)

▼ Autophagy is an important cellular process in maintenance of protein homeostasis. Emerging evidence indicates differential roles of autophagy in cellular function under different pathophysiologic conditions. In some circumstance, autophagy results in cell survival, wheras in other situations it results in cell death. Endotoxin affects neurons in the nucleus tractus solitarii (NTS), baroreceptor afferent terminal site in the brain stem, resulting in cardiovascular depression. The aim of this study was to examine whether modulation of autophagic activity in NTS and other brain regions subserving cardiovascular regulation are associated with cardiovascular depression during experimental endotoxemia. Adult male Sprague-Dawley rats received continuously intraperitoneal infusion via osmotic minipump of lipopolysaccharide (LPS, 2.5 mg/kg/day) or normal saline (NS). Body weight (BW) and systolic blood pressure (SBP) were recorded in animals on days 1, 2, 3, 5, 7, 10, and 14 after LPS treatment. Western bolotting was used to assess the expression of autophagic activity marker, microtubule-associated protein 1 light chain 3 (LC3). Rapamycin (0.55 mg/Kg/day), chemical reported to activate autophagy, was infused continuously into the lateral ventricle of the endotoxemic rats for 7 days via osmotic minipump. Both BW and SBP of rats were decreased in the initial 5 days, followed by a gradual return to baseline after LPS treatment. There was a trend in the decrease in autophagic activity (using the ratio of LC3-â¡/LC3-I as an experimental index) at NTS. However, there is no apparent association between the change in autophagic activity at NTS and the LPS-induced cardiovascular depression. In addition, there was no obvious change in the autophagic activity at RVLM, hypothalamus and hippocampus. Intracranial infusion of rapamycin, a mTOR inhibitor that maintains cellular autophagic activity, resulted in a further enhancement of cardiovascular depression induced by LPS. These results suggest that continuously intraperitoneal infusion via osmotic minipump of LPS result in decreases of body weight and systolic blood pressure. However, the present study provides no direct evidence to support for a cause-and-effect role of autophage at NTS, RVLM, hypothalamus as well as hippocampus in the LPS-induced cardiovascular depression during experimental endotoxemia. Advisors/Committee Members: Chang AYW (chair), Chan JYH (committee member), Shih, C.D (chair), Chuang YC (chair).

Subjects/Keywords: experimental endotoxemia; autophagy; Nucleus Tractus Solitarii

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, C. (2011). The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210111-225538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Chuei-Shiun. “The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia.” 2011. Thesis, NSYSU. Accessed January 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210111-225538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Chuei-Shiun. “The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia.” 2011. Web. 22 Jan 2021.

Vancouver:

Li C. The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 Jan 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210111-225538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li C. The Role of Autophagy at Nucleus Tractus Solitarii in Cardiovascular Depression During Experimental Endotoxemia. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0210111-225538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

5. Aboubaker, Eltayb Abdelwahab Mohamed. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.

Degree: PhD, 2017, University of Aberdeen

URL: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

► Two aspects of trypanosomiasis have been investigated in this study. First, molecular methods were applied to the diagnosis of T.evansi in camels in South Libya.… (more)

▼ Two aspects of trypanosomiasis have been investigated in this study. First, molecular methods were applied to the diagnosis of T.evansi in camels in South Libya. The aim of the study was to determine if FTA card blood sampling and PCR amplification could detect parasites and this be used as tool for diagnosis and epidemiology. Targeted samples of 70 camels were identified on the basis of symptoms of infection and blood was collected on FTA cards. PCR primers and conditions for the amplification of T.evansi DNA were developed on the basis of the literature and a positive control clone grown in the laboratory. The assay found 84.3% of camel samples positive using TBR primers (177bp amplicon) and ITS nested primers (611-1513bp amplicons). This result demonstrated that Surra is endemic in this area, and that T.evansi was the species that was involved. The ITS and TBR loci in the parasites identified in Libya were almost identical to those previously reported in the genbank database, though with some polymorphisms. Dullness and emaciation were the clinical signs of camels infected by trypanosomes, and these two symptoms were significantly related to the 1200bp ITS nested PCR amplicon. These two symptoms can be thus used as a sign an initial diagnosis of T.evansi infection in camels. The second aspect of trypanosomiasis studied was the occurrence of endotoxaemia in infection. The first part of this research investigated endotoxin levels in clinical human African trypasnosomiasis using the Limulus Amoebocyte lysate assay. Endotoxin levels were significantly increased over control individuals in the plasma of T.b.rhodesiense patients. This endotoxaemia was unrelated to infection duration, parasitaemia or clinical stage but resolved after clearance of parasites by drug treatment. In the cerebrospinal fluid there was no significant difference in endotoxin level between early and late stage cases and no relationship to parasite loads. It is argued on the basis of the data that endotoxaemia in trypanosomiasis most likely results from increases in permeability of the gut to endotoxins from gram negative enter bacteria. This conclusion was further supported from a study using cell culture adapted T.brucei and secreted products which gave no evidence of any endotoxin activity. Also samples of an acute experimental mouse infection with T.brucei gave no endotoxin activity, suggesting that this phenomenon requires a more chronic infection in mice. No relationships were found between plasma or CSF endotoxin levels to neurological signs of infection. However the presence of a gross inflammatory clinical symptom, splenomegaly, was associated with endotoxaemia and the concentrations of 3 plasma cytokines associated with the immune response in trypanosome infection were associated with correlated to plasma endotoxin levels. In order to determine the nature of the endotoxin activity, a biosensor cell assay for LPS was used, based on human embryonic kidney cells transfected with TLR4/MD3 and a NF-κB induced alkaline phosphatase reporter…

Subjects/Keywords: 616.9; Trypanosoma; Trypanosoma brucei; Trypanosomiasis; Endotoxemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboubaker, E. A. M. (2017). Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

Chicago Manual of Style (16^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed. “Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.” 2017. Doctoral Dissertation, University of Aberdeen. Accessed January 22, 2021. https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461.

MLA Handbook (7^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed. “Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection.” 2017. Web. 22 Jan 2021.

Vancouver:

Aboubaker EAM. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. [Internet] [Doctoral dissertation]. University of Aberdeen; 2017. [cited 2021 Jan 22]. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461.

Council of Science Editors:

Aboubaker EAM. Trypanosomiasis : molecular diagnosis of Trypanosoma evansi infection and endotoxaemia during Trypanosoma brucei rhodesiense infection. [Doctoral Dissertation]. University of Aberdeen; 2017. Available from: https://eu03.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12152979550005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715461

University of Notre Dame

6. Haifeng Xu. FVII stands on the crossroads of coagulation and inflammation</h1>.

Degree: Chemistry and Biochemistry, 2007, University of Notre Dame

URL: https://curate.nd.edu/show/8g84mk63s3p

► Upregulation of the activated Factor VII (FVIIa)/Tissue Factor (TF) complex, downregulation of natural anticoagulation pathways, and inhibition of fibrinolysis, are major contributors to coagulopathies… (more)

▼ Upregulation of the activated Factor VII (FVIIa)/Tissue Factor (TF) complex, downregulation of natural anticoagulation pathways, and inhibition of fibrinolysis, are major contributors to coagulopathies associated with acute inflammation. Provision of FVIIa, and consequent downstream coagulation-related proteases, also stimulates further inflammatory changes. Thus, the potential protective effects in vivo of a genetic-based reduction in FVII levels have been investigated in a murine model of acute inflammation, viz., lipopolysaccharide (LPS)-induced lethal endotoxemia. Since mice with a total FVII deficiency do not survive the neonatal period, very low-expressing FVII (FVIItTA/tTA) mice, producing sufficient amounts of FVII for survival (ca., 1% of WT FVII), were employed to investigate in vivo pathways involved in the crosstalk between coagulation, inflammation, and survival, consequent to administration of a lethal dose of LPS. The FVIItTA/tTA mice presented with reduced mortality, coagulation, and inflammatory responses when compared to similarly-treated wild-type (WT) mice after administration of LPS. The attenuated inflammatory responses in FVIItTA/tTA mice were associated with downregulation of Egr-1 signaling. Administration, in vivo, of specific inhibitors of FXa and thrombin demonstrated that the inflammatory responses were unaltered in WT mice, but further reduced in FVIItTA/tTA mice. Therefore, a FVII deficiency enhances survival from lethal endotoxemia both through attenuation of inflammatory responses that result directly from reduced FVIIa levels, and, indirectly, from downregulation of coagulation proteases downstream of the FVII-dependent cascade. Keratinocytes from skin tissue are rich sources of tissue factor and are highly associated with skin wound healing. However, studies of the involvement of extrinsic coagulation factors during skin wound healing are still lacking. Here we demonstrated that low FVII mice exhibited impaired skin wound healing. These gene-deficiency mice showed delayed re-epithelialization and reduced inflammatory cell infiltration at wound sites compared with control mice. The reduced inflammatory response in low FVII mice was also demonstrated using a thioglycollate induced inflammatory model. The delayed re-epthelialization in low FVII mice was associated with regulation of Egr-1 as demonstrated by the increased expression level of Egr-1 by FVIIa stimulation in keratinocytes. In vitro, Egr-1 was shown to be essential for FVIIa induced keratinocytes migration and inflammation. In vivo, Egr-1 deficient mice displayed a significant delay of skin wound healing. Advisors/Committee Members: Francis Castellino, Committee Member, Holly Goodson, Committee Member, Patracia Clark, Committee Member, Paul Huber, Committee Member, John Duman, Committee Chair.

Subjects/Keywords: tissue factor; endotoxemia

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APA (6^th Edition):

Xu, H. (2007). FVII stands on the crossroads of coagulation and inflammation</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/8g84mk63s3p

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xu, Haifeng. “FVII stands on the crossroads of coagulation and inflammation</h1>.” 2007. Thesis, University of Notre Dame. Accessed January 22, 2021. https://curate.nd.edu/show/8g84mk63s3p.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xu, Haifeng. “FVII stands on the crossroads of coagulation and inflammation</h1>.” 2007. Web. 22 Jan 2021.

Vancouver:

Xu H. FVII stands on the crossroads of coagulation and inflammation</h1>. [Internet] [Thesis]. University of Notre Dame; 2007. [cited 2021 Jan 22]. Available from: https://curate.nd.edu/show/8g84mk63s3p.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xu H. FVII stands on the crossroads of coagulation and inflammation</h1>. [Thesis]. University of Notre Dame; 2007. Available from: https://curate.nd.edu/show/8g84mk63s3p

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

7. Brandon, Christopher Inman. The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/21759

► The primary objectives stemming from this project were to characterize fully both the equineadenosine A2A (eA2A-R) and A3 (eA3-R) receptors to determine if they would… (more)

Subjects/Keywords: Adenosine; Receptor; Equine; Endotoxemia; Heterologous Expression System

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brandon, C. I. (2014). The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/21759

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Brandon, Christopher Inman. “The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors.” 2014. Thesis, University of Georgia. Accessed January 22, 2021. http://hdl.handle.net/10724/21759.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Brandon, Christopher Inman. “The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors.” 2014. Web. 22 Jan 2021.

Vancouver:

Brandon CI. The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10724/21759.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Brandon CI. The cloning and pharmacological characterization of the equine adenosine A2a and A3 receptors. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/21759

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Harvey, Mordecai Micah. Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation.

Degree: PhD, Human Nutrition, Foods, and Exercise, 2017, Virginia Tech

URL: http://hdl.handle.net/10919/78235

► The prevalence of obesity and type II diabetes is increasing. Although exercise is widely accepted for prevention and treatment, evidence of resistance to exercise in… (more)

Subjects/Keywords: endotoxemia; skeletal; muscle; exercise; electric; stimulation

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harvey, M. M. (2017). Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/78235

Chicago Manual of Style (16^th Edition):

Harvey, Mordecai Micah. “Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation.” 2017. Doctoral Dissertation, Virginia Tech. Accessed January 22, 2021. http://hdl.handle.net/10919/78235.

MLA Handbook (7^th Edition):

Harvey, Mordecai Micah. “Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation.” 2017. Web. 22 Jan 2021.

Vancouver:

Harvey MM. Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation. [Internet] [Doctoral dissertation]. Virginia Tech; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10919/78235.

Council of Science Editors:

Harvey MM. Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation. [Doctoral Dissertation]. Virginia Tech; 2017. Available from: http://hdl.handle.net/10919/78235

9. Oliveira, Edson Mendes de. New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance.

Degree: PhD, Análises Clínicas, 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01062015-162141/ ;

►

Chronic low-grade endotoxemia is an important player in obesity and insulin resistance associated to a high-fat diet (HFD). On the other hand, although it is… (more)

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Chronic low-grade endotoxemia is an important player in obesity and insulin resistance associated to a high-fat diet (HFD). On the other hand, although it is known that intense endotoxemia and infection reduce appetite and induce intense catabolism, leading to weight loss during the acute inflammatory phase, the late effects of an intense endotoxemia were previously unexplored. Here we report that, besides the concurrent effects, multiple and intense endotoxemia causes long lasting biochemical alterations in the adipose tissue that intensify the harmful effects of a HFD. Mice submitted to multiple and severe endotoxemia had increased the adipose tissue expression of TLR-4, CD14 and SAA3, remaining altered after one week in recovery. When associated to a HFD, mice previously submitted to acute endotoxemia showed a more severe weight gain and impaired insulin sensitivity. Adopting the HFD as an obesogenic stimulus, we evaluated the participation of the protein serum amyloid A (SAA) in obesity development. Using a SAA-targeted antisense oligonucleotide, we observed that the depletion of SAA prevented metabolic alterations, endotoxin elevation, weight gain and insulin resistance in a diet-induced obesity protocol. Inadequate sleep is another important factor to be considered in the obesity epidemic. We found that sleep restriction (SR) causes biochemical and morphological alterations in mice adipose tissue. The levels of serum resistin and the adipose tissue mRNA expression of resistin, TNF-α and IL-6 were increased after SR. When associated to a HFD, mice previously submitted to SR gained more weight with increased macrophage infiltration in the epididymal adipose tissue, and insulin resistance. SAA is also part of the initial biochemical alterations caused by SR. It was observed that the expression of SAA in liver and adipose tissue is upregulated, with return to baseline when sleep is restored. Furthermore, 48 hours of total sleep restriction in healthy human volunteers also caused a serum elevation in SAA concentrations. Considering that SAA induces cell proliferation, we suggest that situations with an increase in SAA production and the consecutive preadipocyte proliferation would prime the adipose tissue to further adipocyte differentiation and hypertrophy. Furthermore, we suggest that SAA alter LPS signaling, possibly inhibiting its clearance. The mechanism associating inflammation and obesity is complex and encompass a diversity of factors; the inflammatory protein SAA may be one of them. In conclusion, our data describes the relationship between SAA, acute inflammation, sleep restriction and obesity.

Endotoxemia crônica de baixo grau tem um importante papel na obesidade e resistência à insulina associada a uma ração hiperlipídica. Por outro lado, embora se saiba que a endotoxemia intensa e infecção reduzam o apetite e induzam a um intenso catabolismo, conduzindo a perda de peso durante a fase aguda da inflamação, os efeitos tardios da endotoxemia intensa nunca foram explorados. Aqui mostramos que, além…

Advisors/Committee Members: Campa, Ana.

Subjects/Keywords: Acute inflammation; Adipócito; Adipocyte; Endotoxemia; Endotoxemia; Fase aguda; Inflamação; Inflammation; Restrição de sono; Sleep restriction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira, E. M. d. (2015). New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01062015-162141/ ;

Chicago Manual of Style (16^th Edition):

Oliveira, Edson Mendes de. “New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance.” 2015. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01062015-162141/ ;.

MLA Handbook (7^th Edition):

Oliveira, Edson Mendes de. “New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance.” 2015. Web. 22 Jan 2021.

Vancouver:

Oliveira EMd. New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01062015-162141/ ;.

Council of Science Editors:

Oliveira EMd. New insights into the role of serum amyloid A (SAA) on obesity and insulin resistance. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01062015-162141/ ;

10. Felipe Hertzing Farias. Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental.

Degree: 2012, Universidade do Estado de Santa Catarina

URL: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3293

►

A endotoxemia Ã um dos problemas com maiores dificuldades de diagnÃstico e tratamento, devido ao desencadeamento de uma resposta imunolÃgica exacerbada, culminando com o envolvimento… (more)

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A endotoxemia Ã um dos problemas com maiores dificuldades de diagnÃstico e tratamento, devido ao desencadeamento de uma resposta imunolÃgica exacerbada, culminando com o envolvimento de mÃltiplos ÃrgÃos. A cetamina possui efeitos anti-inflamatÃrios em diversas espÃcies, entretanto, em felinos esse ainda nÃo foi comprovado. Objetivou-se neste estudo, avaliar os efeitos clÃnicos e anti-inflamatÃrios da administraÃÃo de cetamina, em doses subanestÃsicas, previamente ou apÃs a induÃÃo da endotoxemia em felinos. Foram utilizados nove gatos machos, hÃgidos, sendo autocontroles. ApÃs jejum alimentar de 12 horas e hÃdrico de seis horas, administrou-se dexmedetomidina (5 ug kg-1) e butorfanol (0,3 mg kg-1), pela via intramuscular, para inserÃÃo de um cateter na veia jugular e na veia cefÃlica. No dia seguinte, os animais foram sorteados e alocados em trÃs grupos: lipopolissacarÃdeo (LPS, n=9), os quais receberam bolus de soluÃÃo de NaCl 0,9% (0,05 ml kg-1), seguida por infusÃo contÃnua (IC) de LPS (2 ug kg-1 h-1) na taxa de 2 ml kg-1 h-1, durante quatro horas; cetamina/LPS (C/LPS, n=9), os quais receberam bolus de cetamina (0,5 mg kg-1), seguido da IC de cetamina (10 ug kg-1 min-1) e LPS (2 ug kg-1 h-1) na taxa de 2 ml kg-1 h-1 por duas horas e, apÃs, infusÃo de LPS (2 ug kg-1 h-1) na mesma taxa por mais duas horas; LPS/Cetamina (LPS/C, n=9), os quais receberam IC de LPS (2 ug kg-1 h-1), por duas horas e, logo apÃs bolus de cetamina (0,5 mg kg-1), IC da mesma (10 ug kg-1 min-1) e LPS (2 ug kg-1 h-1) na mesma taxa por duas horas. Todos os tratamentos foram diluÃdos em NaCl 0,9% e administrados pela via intravenosa. Os parÃmetros foram mensurados nos momentos: M-15, antes do inÃcio da IC de LPS e 5 (M5) 30 (M30), 60 (M60), 90 (M90), 120 (M120), 150 (M150), 180 (M180), 240 (M240), 360 (M360), 480 (M480), 600 (M600) e 720 (M720) minutos apÃs o inÃcio da IC. AlÃm disso, avaliou-se os parÃmetros clÃnicos, hemograma e bioquÃmico apÃs o inÃcio da IC de LPS. A frequÃncia cardÃaca (FC) foi maior de M5 a M120 no C/LPS e menor de M150 a M240 e M600 a M720 no LPS em relaÃÃo aos outros tratamentos. De M5 no C/LPS, de M60 no LPS/C e de M90 no LPS atÃ a M720 a FC foi maior em relaÃÃo a M-15. A pressÃo arterial sistÃlica foi menor em todos os grupos de M360 a M720 em relaÃÃo ao basal. Em todos os tratamento observou-se aumento da temperatura retal de M60 a M600 em relaÃÃo a M-15. Os valores de glicose e lactato elevaram-se de M120 a M360 em relaÃÃo ao basal nos trÃs grupos. Os leucÃcitos apresentaram-se diminuÃdos de M60 a M360 em todos os tratamentos em relaÃÃo a M-15. Os triglicerÃdeos apresentaram-se elevados de M720 a M1440 em relaÃÃo ao basal em todos os grupos. Conclui-se que doses subanestÃsicas a cetamina apresentou mÃnimas alteraÃÃes nos parÃmetros cardiovasculares, com aumento da frequÃncia cardÃaca. A cetamina administrada previamente ou apÃs a induÃÃo da endotoxemia nÃo amenizou ou reverteu os efeitos deletÃrios dessa, em gatos submetidos Ã endotoxemia experimental atravÃs da infusÃo contÃnua de lipopolissacarÃdeo

Endotoxemia…

Advisors/Committee Members: Nilson Oleskovicz.

Subjects/Keywords: endotoxemia; cetamina; subanestÃsica; lipopolissacarÃdeo; gatos; endotoxemia; ketamine; subanesthetic; lipopolysaccharide; cats; MEDICINA VETERINARIA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Farias, F. H. (2012). Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental. (Thesis). Universidade do Estado de Santa Catarina. Retrieved from http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Farias, Felipe Hertzing. “Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental.” 2012. Thesis, Universidade do Estado de Santa Catarina. Accessed January 22, 2021. http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Farias, Felipe Hertzing. “Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental.” 2012. Web. 22 Jan 2021.

Vancouver:

Farias FH. Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental. [Internet] [Thesis]. Universidade do Estado de Santa Catarina; 2012. [cited 2021 Jan 22]. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3293.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Farias FH. Efeitos da cetamina em doses subanestÃsicas em gatos, submetidos Ã endotoxemia experimental. [Thesis]. Universidade do Estado de Santa Catarina; 2012. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3293

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Barbeiro, Denise Frediani. Participação da célula B-1 na resposta inflamatória ao lipopolissacáride.

Degree: PhD, Emergências Clínicas, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-08032010-163256/ ;

►

A sepse é a Síndrome da Resposta Inflamatória Sistêmica decorrente de uma infecção por gram positivos/negativos, fungos ou vírus. É caracterizada por alta liberação de… (more)

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A sepse é a Síndrome da Resposta Inflamatória Sistêmica decorrente de uma infecção por gram positivos/negativos, fungos ou vírus. É caracterizada por alta liberação de mediadores inflamatórios podendo levar à morte. As células B-1 são encontradas em cavidades peritoneal e pleural de camundongos e sua origem e função ainda não são completamente conhecidas. Apresentam marcadores de superfície de linhagem mielóide e linfóide e migram para focos inflamatórios comportando-se como macrófagos. Objetivo: investigar o papel da célula B-1 na resposta inflamatória após estímulo com lipopolissacáride (LPS) in vitro e in vivo. Métodos: TNF-, IL-6, IL-10 (ELISA) e nitrito (Griess) foram dosados em sobrenadante de cultura celular (106 cel./ml). As células em cultura receberam por 24h de estímulo com 10 g/mL de LPS de Escherichia coli (026:B6 Sigma®). Foram realizados os seguintes grupos cultura de célula B-1 (Balb/c), cultura de macrófagos de linhagem (RAW 264.7) coculturas (macrófagos de linhagem RAW 264.7 e células B-1 (Balb/c, C57BL/6 e C57BL/6 IL-10 -/-), e células peritoneais de camundongos Balb/c e Balb/Xid (imunodeficiente em célula B-1) A endotoxemia foi induzida com injeção de LPS 15 mg/kg (i.p.) em camundongos Balb/c e Balb/Xid. Foram quantificados, TNF-, IL-6, IL-10 e nitrito em soro, pulmão e intestino dos animais após 1,5, 4 e 6 horas após a injeção de LPS. Ensaios de inoculação de células B-1 (Balb/c) em camundongos Balb/Xid foram realizados, e curva de sobrevida foi analisada após indução de endotoxemia. Resultados: Após o estímulo com LPS, células B-1 produziram IL-10 e a presença destas células em cocultura com macrófago promoveu a diminuição na produção de TNF-, IL-6, Nitrito e aumento de IL-10. Contudo, célula B-1 (IL-10 -/-) em cocultura com macrófagos, não inibem a produção de mediadores pro inflamatórios. Análise com macrófagos peritoneais de camundongo Balb/Xid e Balb/c após estímulo com LPS em cultura mostrou reprodução do fenômeno encontrado com os experimentos com cultura de célula imortalizada, isto é, maior produção de TNF-, IL-6 e NO em Balb/Xid (B-1 deficiente). Os estudos in vivo mostraram 60% de mortalidade em camundongo Balb/Xid comparando com Balb/c (0%) após 16 horas de injeção de LPS. Nos animais Balb/Xid encontramos padrão pro inflamatório exacerbado com maiores concentrações de TNF-, IL-6 e menores concentrações de IL-10 no plasma e tecidos quando comparamos com Balb/c. Conclusões: Nossos dados mostraram que a presença de células B-1 promoveram diminuição de mediadores pro inflamatórios e aumento de IL-10 em coculturas com macrófagos e que a modulação da resposta inflamatória pode ser devida a secreção de IL-10 pela célula B-1. Este padrão de resposta pro inflamatória se repete in vivo e é a possível causadora da maior taxa de mortalidade em camundongos da linhagem Balb/Xid.

Sepsis syndrome is caused by inappropriate immune activation due to bacteria and bacterial components released during infection. This syndrome is the leading cause of death in intensive care units. Specialized…

Advisors/Committee Members: Soriano, Francisco Garcia.

Subjects/Keywords: B-Lymphocytes/immunology; Endotoxemia; Endotoxemia; Inflamação; Inflammation; Linfócitos B/imunologia; Lipopolissacarídeos/imunologia; Lipopolysaccharides/immunology; Macrófagos; Macrophages

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barbeiro, D. F. (2009). Participação da célula B-1 na resposta inflamatória ao lipopolissacáride. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5159/tde-08032010-163256/ ;

Chicago Manual of Style (16^th Edition):

Barbeiro, Denise Frediani. “Participação da célula B-1 na resposta inflamatória ao lipopolissacáride.” 2009. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/5/5159/tde-08032010-163256/ ;.

MLA Handbook (7^th Edition):

Barbeiro, Denise Frediani. “Participação da célula B-1 na resposta inflamatória ao lipopolissacáride.” 2009. Web. 22 Jan 2021.

Vancouver:

Barbeiro DF. Participação da célula B-1 na resposta inflamatória ao lipopolissacáride. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-08032010-163256/ ;.

Council of Science Editors:

Barbeiro DF. Participação da célula B-1 na resposta inflamatória ao lipopolissacáride. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/5/5159/tde-08032010-163256/ ;

12. Saraiva, Adriana Paiva Camargo. Periodontite e aterosclerose: a busca de evidências.

Degree: PhD, Enfermagem Fundamental, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-26042010-102150/ ;

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As doenças cardiovasculares (DCV) de origem aterosclerótica estão entre as principais causas de morbimortalidade cardiovascular. A periodontite, por meio de bacteremia e endotoxemia, tem sido… (more)

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As doenças cardiovasculares (DCV) de origem aterosclerótica estão entre as principais causas de morbimortalidade cardiovascular. A periodontite, por meio de bacteremia e endotoxemia, tem sido apontada como possível fator de risco para início e progressão da aterosclerose. A proposta desta revisão foi buscar, reunir e analisar evidências científicas atuais de nível I e II sobre a associação entre periodontite e aterosclerose em seres humanos. As bases de dados consultadas foram: Medline (Medical Literature Analysis and Retrieval System), registro Cochrane de ensaios controlados (Cochrane Central Register of Controlled Trials - Central) e registro Cochrane de revisões sistemáticas (Cochrane Databasis of Systematic Reviews - CDSR). Foram identificados 532 estudos, sendo 22 elegíveis, dos quais nove atenderam aos critérios de inclusão. A amostra foi composta por 100% de ensaios clínicos randomizados controlados (ECRC). Oito estudos (89%) avaliaram o efeito do tratamento periodontal quanto aos parâmetros clínicos periodontais e marcadores relacionados à fisiopatologia da aterosclerose, em três destes (37,5%) o efeito do tratamento periodontal foi testado com antibioticoterapia e um (11%) avaliou o efeito somente da antibioticoterapia. Sete (78%) avaliaram níveis séricos de proteína C-reativa (PCR) antes e após o tratamento periodontal, em quatro (57%) houve redução deste marcador nas avaliações posteriores a seis semanas. Cinco (55,5%) avaliaram níveis séricos de interleucina-6 (IL-6), dos quais três (60%) relataram redução após mais de um mês do tratamento periodontal. Fatores lipídicos foram avaliados em três estudos (33%) e todos relataram melhoria significativa após tratamento periodontal. Evidências científicas de nível II apontam que a periodontite parece provocar alterações nos marcadores sistêmicos relacionados à fisiopatologia da aterosclerose podendo o tratamento periodontal ser benéfico para controlar marcadores séricos de risco para aterosclerose, com resultados significativos em casos mais severos de periodontite e para os marcadores lipídicos.

Cardiovascular diseases (CVD) caused by atherosclerosis are among the leading causes of cardiovascular morbity and mortality. Periodontitis through bacteremia and endotoxemia has been identified as a possible risk factor for initiation and progression of atherosclerosis. The purpose of this review was to seek, gather and analyze evidence of level I and II available in the literature on the association between periodontitis and atherosclerosis in the last ten years. The databases were consulted: Medline (Medical Literature Analysis and Retrieval System), Cochrane register of controlled trials (Cochrane Central Register of Controlled Trials - Central) and record Cochrane systematic reviews (Cochrane Database of Systematic Reviews - CDSR). We identified 532 studies, of which 22 eligible, of which 9 met the inclusion criteria. The sample consisted of 100% of randomized controlled trials (ECRC). Eight studies (89%) evaluated the effect of periodontal treatment on the…

Advisors/Committee Members: Veiga, Eugenia Velludo.

Subjects/Keywords: Aterosclerose; Atherosclerosis; Endothelial function; Endotoxemia; Endotoxemia; Evidence-Based Medicine; Função endotelial; Inflamação; Inflammation; Periodontite; Periodontits; Prática Baseada em Evidências

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saraiva, A. P. C. (2010). Periodontite e aterosclerose: a busca de evidências. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/22/22132/tde-26042010-102150/ ;

Chicago Manual of Style (16^th Edition):

Saraiva, Adriana Paiva Camargo. “Periodontite e aterosclerose: a busca de evidências.” 2010. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/22/22132/tde-26042010-102150/ ;.

MLA Handbook (7^th Edition):

Saraiva, Adriana Paiva Camargo. “Periodontite e aterosclerose: a busca de evidências.” 2010. Web. 22 Jan 2021.

Vancouver:

Saraiva APC. Periodontite e aterosclerose: a busca de evidências. [Internet] [Doctoral dissertation]. University of São Paulo; 2010. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-26042010-102150/ ;.

Council of Science Editors:

Saraiva APC. Periodontite e aterosclerose: a busca de evidências. [Doctoral Dissertation]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/22/22132/tde-26042010-102150/ ;

13. Lorigados, Clara Batista. Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos.

Degree: PhD, Distúrbios do Crescimento Celular, Hemodinâmicos e da Hemostasia, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27082014-142206/ ;

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Pacientes sépticos com disfunção miocárdica apresentam mortalidade significativamente superior comparados aos sépticos sem alteração cardiovascular. Vários mecanismos contribuem para disfunção orgânica na sepse, como diminuição… (more)

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Pacientes sépticos com disfunção miocárdica apresentam mortalidade significativamente superior comparados aos sépticos sem alteração cardiovascular. Vários mecanismos contribuem para disfunção orgânica na sepse, como diminuição de perfusão tecidual. A sepse está relacionada a alterações na microcirculação e na permeabilidade capilar que apresentam papel fundamental na fisiopatologia das disfunções orgânicas. O objetivo do estudo foi analisar o papel da pressão de perfusão coronariana como fator determinante do fluxo sanguíneo na microcirculação miocárdica e sua correlação com a função cardíaca sistólica e diastólica em ratos endotoxêmicos. Ratos machos, Wistar, 300g, receberam LPS 10 mg/kg ip. Após uma hora e meia da injeção, um cateter de pressão-volume foi locado no VE e um cateter pressórico na artéria femoral para aquisição dos parâmetros hemodinâmicos cardíacos e sistêmicos respectivamente. Foram estudados os ratos que apresentaram choque endotoxêmico (PAM <= 65 mmHg). Um grupo foi tratado com norepinefrina iv e outro com araminol iv, para atingir PAM de 85 mmHg. Para o estudo do fluxo sanguíneo, microesferas amarelas (15 ?m) foram injetadas no VE para analisar a microcirculação cardíaca. O coração foi analisado em três partes: VD, região epicárdica e região subendocárdica do VE. O estudo demonstrou uma redução de 58% na PPC e de 50% no fluxo miocárdico nos ratos com choque endotoxêmico. Houve queda de 34% na dP/dt max e 15% na dP/dt min comparados ao controle. Os parâmetros de função cardíaca sistólica volume-independentes, Ees e dP/dtmax / EDV, também apresentaram redução. Nos ratos tratados com norepinefrina, observou-se aumento da PPC (38 ± 2 vs. 59 ± 3 mmHg, LPS vs. LPS+NOR) e do fluxo sanguíneo miocárdico (2,0 ± 0,6 vs. 6,2 ± 0,8 mL/min.g tecido, LPS vs. LPS+NOR) e os índices de função cardíaca sistólica e diastólica mostraram recuperação. A PPC apresentou correlação significativa com o fluxo sanguíneo subendocárdico do VE.Os dados demonstraram que os animais em choque endotoxêmico e, portanto com PPC baixa, apresentaram redução no fluxo sanguíneo na microcirculação miocárdica, sobretudo no ventrículo direito e na região subendocárdica de VE. Isto se correlacionou com a disfunção cardíaca sistólica e diastólica. Ao elevar-se a PPC com a utilização de norepinefrina, houve aumento do fluxo sanguíneo miocárdico acompanhado de recuperação dos índices de função cardíaca

Septic patients with myocardial dysfunction have higher mortality compared to patients with no cardiovascular alteration. The aim of the present study was to investigate the role of coronary driving pressure as determinant factor of myocardial microcirculation blood flow and its correlation with the cardiac function in endotoxemic heart. Wistar rats, male, 300g were used. Endotoxemia was induced by the injection of 10 mg / kg ip LPS. After 1.5 h of injection, hemodynamic evaluation was performed. It was studied rats with MAP <= 65 mmHg. Norepinephrine and araminol were used to handle MAP to 85 mmHg. Millar catheter was placed in the left…

Advisors/Committee Members: Velasco, Irineu Tadeu.

Subjects/Keywords: Cardiovascular diseases; Circulação coronária; Coronary circulation; Doenças cardiovasculares; Endotoxemia; Endotoxemia; Perfusão; Perfusion; Ratos Wistar; Rats Wistar

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lorigados, C. B. (2014). Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27082014-142206/ ;

Chicago Manual of Style (16^th Edition):

Lorigados, Clara Batista. “Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos.” 2014. Doctoral Dissertation, University of São Paulo. Accessed January 22, 2021. http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27082014-142206/ ;.

MLA Handbook (7^th Edition):

Lorigados, Clara Batista. “Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos.” 2014. Web. 22 Jan 2021.

Vancouver:

Lorigados CB. Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos. [Internet] [Doctoral dissertation]. University of São Paulo; 2014. [cited 2021 Jan 22]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27082014-142206/ ;.

Council of Science Editors:

Lorigados CB. Estudo da relação entre pressão de perfusão coronariana e função cardíaca em ratos endotoxêmicos. [Doctoral Dissertation]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27082014-142206/ ;

NSYSU

14. Chang, Pi-Shan. Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia.

Degree: Master, Biological Sciences, 2001, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0626101-123602

► The components of SAP signals of low- frequency (LF: 0.08-0.15 Hz) and very low- frequency (VLF: 0.00-0.08 Hz) related with vasomotor tone that reflects the… (more)

Subjects/Keywords: Endotoxemia; Spectral analysis; NRVL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chang, P. (2001). Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0626101-123602

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chang, Pi-Shan. “Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia.” 2001. Thesis, NSYSU. Accessed January 22, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0626101-123602.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chang, Pi-Shan. “Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia.” 2001. Web. 22 Jan 2021.

Vancouver:

Chang P. Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia. [Internet] [Thesis]. NSYSU; 2001. [cited 2021 Jan 22]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0626101-123602.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chang P. Role of Glutamatergic Neurotransmission at the Nucleus Reticularis Ventrolateralis during Experimental Endotoxemia. [Thesis]. NSYSU; 2001. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0626101-123602

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Félix de Melo, Juliana. Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal .

Degree: 2007, Universidade Federal de Pernambuco

URL: http://repositorio.ufpe.br/handle/123456789/7500

► A endotoxemia está associada a uma ampla e inespecífica cascata de eventos. Isto resulta na secreção de uma variedade de potentes mediadores próinflamatórios e citocinas… (more)

Subjects/Keywords: Endotoxemia; Desnutrição; Sistema imune.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Félix de Melo, J. (2007). Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal . (Thesis). Universidade Federal de Pernambuco. Retrieved from http://repositorio.ufpe.br/handle/123456789/7500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Félix de Melo, Juliana. “Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal .” 2007. Thesis, Universidade Federal de Pernambuco. Accessed January 22, 2021. http://repositorio.ufpe.br/handle/123456789/7500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Félix de Melo, Juliana. “Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal .” 2007. Web. 22 Jan 2021.

Vancouver:

Félix de Melo J. Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal . [Internet] [Thesis]. Universidade Federal de Pernambuco; 2007. [cited 2021 Jan 22]. Available from: http://repositorio.ufpe.br/handle/123456789/7500.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Félix de Melo J. Atividade oxidante-antioxidante de macrófagos alveolares em ratos endotoxêmicos submetidos à desnutrição neonatal . [Thesis]. Universidade Federal de Pernambuco; 2007. Available from: http://repositorio.ufpe.br/handle/123456789/7500

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

16. Vuaden, Fernanda Cenci. Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares.

Degree: 2010, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/24859

► O ATP extracelular atua como um mediador pró-inflamatório e a adenosina tem sido descrita por suas propriedades anti-inflamatórias. O papel desse nucleosídeo no controle da… (more)

▼ O ATP extracelular atua como um mediador pró-inflamatório e a adenosina tem sido descrita por suas propriedades anti-inflamatórias. O papel desse nucleosídeo no controle da inflamação ocorre principalmente via receptores A2A. As ecto-enzimas responsáveis pelo controle dos níveis de nucleotídeos e nucleosídeos extracelulares são as ectonucleotidases. Esse grupo de ecto-enzimas é composto pela família das ecto-nucleosídeo trifosfato difosfoidrolases (E-NTPases), a família das ecto-nucleotídeo pirofosfatase/fosfodiesterases (E-NPP) e pela ecto-5'-nucleotidase. Considerando o papel que os nucleotídeos e nucleosídeos exercem durante eventos inflamatórios, e a importância das ectonucleotidases na manutenção dos seus níveis extracelulares, o objetivo deste trabalho foi investigar o efeito da indução do modelo de endotoxemia sobre as atividades ectonucleotidásicas em diferentes tipos e frações celulares. O envolvimento do receptor A2A sobre esses parâmetros também foi avaliado, através da utilização do agonista específico do receptor A2A, GCS-21680, no intuito de melhor compreender o envolvimento do sistema purinérgico no processo inflamatório. Para a indução do modelo de endotoxemia os ratos foram injetados intraperitonialmente (i.p.) com 2 mg/kg de LPS e os camundongos foram injetados i.p. com 12 mg/kg de LPS e/ou CGS-21680 (0,5 mg/kg, i.p.). As atividades ectonucleotidásicas foram determinadas em plaquetas de ratos, linfócitos de linfonodos mesentéricos de camundongos e preparações de membranas renais de camundongos. A análise da expressão das ectonucleotidases foi realizada através de RT-PCR. Os resultados demonstraram uma diminuição na hidrólise de ATP, ADP, AMP e 5'TMP em plaquetas de ratos após a indução da endotoxemia (28%, 28%, 30% e 26%, respectivamente). A contagem e a agregação plaquetária também foram diminuídas (40% e 55%, respectivamente). Em linfócitos de camundongos, houve um aumento na hidrólise de ATP, ADP, AMP e 5'TMP 24 horas após a injeção de LPS (178%, 111%, 207% e 62%, respectivamente) e 48 horas após a indução do modelo (135%, 178%, 121% e 116%, respectivamente). Quando o agonista do receptor A2A de adenosina, CGS-21680, foi co-administrado com o LPS, esse aumento foi revertido para as hidrólises de ATP, AMP e 5'-TMP. Em membranas renais de camundongos, os resultados demonstraram um aumento na hidrólise de ATP e 5'-TMP 48 horas após a injeção de LPS (48% e 47%, respectivamente) e a hidrólise do AMP foi diminuída 24 horas após a indução do modelo (40%). Os níveis extracelulares de ATP e adenosina foram diminuídos nos grupos tratados quando comparados ao controle em preparações de membranas renais. Os resultados deste trabalho indicam que as ectonucleotidases são moduladas pela endotoxemia em diferentes frações biológicas, sugerindo que as alterações observadas são conseqüência da resposta inflamatória. Em linfócitos esse efeito foi revertido pela ativação dos receptores A2A. Esses dados demonstram a interação cruzada entre a ativação dos receptores A2A de adenosina e as enzimas que modulam a… Advisors/Committee Members: Bonan, Carla Denise.

Subjects/Keywords: Endotoxemia; Receptor A2A de adenosina; Ectonucleotidases; Sistema purinérgico; Lipopolissacarídeos

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vuaden, F. C. (2010). Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/24859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vuaden, Fernanda Cenci. “Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares.” 2010. Thesis, Universidade do Rio Grande do Sul. Accessed January 22, 2021. http://hdl.handle.net/10183/24859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vuaden, Fernanda Cenci. “Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares.” 2010. Web. 22 Jan 2021.

Vancouver:

Vuaden FC. Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2010. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10183/24859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vuaden FC. Influência da endotoxemia e da ativação dos receptores A2A de adenosina sobre a hidrólise de nucleotídeos extracelulares. [Thesis]. Universidade do Rio Grande do Sul; 2010. Available from: http://hdl.handle.net/10183/24859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

17. Grunewald, Zachary Ian. The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions.

Degree: 2017, University of Georgia

URL: http://hdl.handle.net/10724/36748

► Obesity is characterized by inflammation and insulin resistance. Lipopolysaccharide (LPS) derived from Gram-negative gut bacteria increases with obesity (metabolic endotoxemia) and has been identified as… (more)

Subjects/Keywords: Metabolic endotoxemia; inflammation; insulin resistance; endocannabinoid; obesity; LPS; cardiovascular

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Grunewald, Z. I. (2017). The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/36748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Grunewald, Zachary Ian. “The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions.” 2017. Thesis, University of Georgia. Accessed January 22, 2021. http://hdl.handle.net/10724/36748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Grunewald, Zachary Ian. “The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions.” 2017. Web. 22 Jan 2021.

Vancouver:

Grunewald ZI. The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions. [Internet] [Thesis]. University of Georgia; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10724/36748.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Grunewald ZI. The role of cannabinoid receptor type-1 in metabolic endotoxemia-induced metabolic dysfunctions. [Thesis]. University of Georgia; 2017. Available from: http://hdl.handle.net/10724/36748

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal da Bahia

18. Tania Tavares Rodriguez. Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico.

Degree: 2005, Universidade Federal da Bahia

URL: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=381

► A identificação do óxido nítrico (NO) dentro do hipotálamo e da glândula pituitária sugere que o NO participa como molécula moduladora da atividade do eixo… (more)

▼ A identificação do óxido nítrico (NO) dentro do hipotálamo e da glândula pituitária sugere que o NO participa como molécula moduladora da atividade do eixo hipotálamo-pituitária. Os hormônios tireoidianos, por sua vez, regulam a atividade hipotalâmica da sintase do NO (NOS). O objetivo deste trabalho foi avaliar os efeitos da injeção prévia de Nv-nitro-L-arginina metil ester (L-NAME), inibidor da NOS, e de L-Arginina (L-Arg), substrato para a geração de NO, na secreção de prolactina (PRL) e corticosterona (CORT), na expressão da proteína c-Fos hipotalâmica e na redistribuição de células imunocompetentes (neutrófilos, linfócitos e seus subtidos T, CD4+ e CD8+) do sangue periférico induzido pelo estresse de imobilização (IMO) e induzido pela resposta inflamatória através do lipopolissacaróideo (LPS) em ratos tireoidectomizados. Ratos Wistar (220-280g), tireoidectomizados (TX) ou falsos operados (N), (n = 10/15 por grupo), foram tratados com injeções intraperitoneal (i.p.) de L-NAME (10 mg/Kg) ou L-Arg (200mg/Kg), 30 minutos antes da indução do estresse ou da injeção de LPS, 250 mg/100 g peso corporal, i.p.. Os animais-controle receberam um volume similar de solução salina (SL) 0,9% i.p.. Amostras de sangue para análise hormonal foram coletadas nos tempos ?30min (basal), 0min (após injeção de L-NAME, L-Arg ou SL) e após 5, 15, 30, 60, 120 e 240 min de estresse ou endotoxemia. Cérebros de ratos para detecção da proteína c-Fos, através de imunohistoquímica, foram coletados nos tempos basais (-30 min), e após 30 min de injeção de L-NAME, L-Arg ou SL, e 120 min de endotoxemia ou estresse de IMO. Amostras de sangue para análise dos leucócitos foram coletadas antes e depois das drogas e após 240min de estresse ou endotoxemia. Foram dosados os hormônios por radioimunoensaio e foram analisados os subtipos de linfócitos por citometria de 18 fluxo. Os resultados mostram que o hipotireoidismo bloqueia parcialmente a secreção de PRL e CORT durante a IMO e a endotoxemia. Nos ratos N, o NO aumenta a secreção de PRL durante a endotoxemia, enquanto nos ratos hipotireoidianos o NO tem um efeito contrário. Embora o NO não seja um fator limitante na liberação de PRL induzida pelo estresse, a sobrecarga de L-Arg modula a secreção de PRL, aumentando-a temporariamente em ambos os grupos. O NO estimula a secreção de CORT durante a endotoxemia e o estresse de IMO, porém concentrações elevadas de NO reduzem a liberação de CORT diretamente pela glândula adrenal. A atividade da NOS é regulada para baixo nos ratos hipotireoidianos, portanto o hipotireoidismo modula negativamente o eixo hipotálamo-pituitário-adrenal. O hipotireoidismo reduz a resposta inflamatória induzida pelo LPS e o NO age como uma molécula antiadesiva nesse processo. O NO modula o tráfico leucocitário que resulta na redistribuição de leucócitos entre o sangue e outros compartimentos imunes. A modulação da distribuição das células imunes no estresse agudo e na endotoxemia é uma resposta adaptativa para potencializar a vigilância imunológica e aumentar a capacidade do sistema… Advisors/Committee Members: José Antunes Rodrigues, Manuel Herminio de Aguiar Oliveira, Márcia Tosta Xavier, João Palermo Neto, Maria José Pedreira Ramalho, Laura M. Vivas.

Subjects/Keywords: óxido nitrico; endotoxemia; estresse; IMUNOLOGIA; hipotireoidismo; avaliação neuroimunoendócrina

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodriguez, T. T. (2005). Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico. (Thesis). Universidade Federal da Bahia. Retrieved from http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=381

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodriguez, Tania Tavares. “Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico.” 2005. Thesis, Universidade Federal da Bahia. Accessed January 22, 2021. http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=381.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodriguez, Tania Tavares. “Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico.” 2005. Web. 22 Jan 2021.

Vancouver:

Rodriguez TT. Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico. [Internet] [Thesis]. Universidade Federal da Bahia; 2005. [cited 2021 Jan 22]. Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=381.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodriguez TT. Avaliação neuroimunoendócrina de ratos hipotireoidianos submetidos ao estresse de imobilização e a endotoxemia: participação da L-arginina e da sintase do óxido nítrico. [Thesis]. Universidade Federal da Bahia; 2005. Available from: http://www.bibliotecadigital.ufba.br/tde_busca/arquivo.php?codArquivo=381

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Aletéia Marieta da Silva Seabra. Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia.

Degree: 2012, Universidade Federal do Triângulo Mineiro

URL: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=186 ; http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=187

► A melatonina, principal hormônio produzido pela pineal, pode também ser produzida por outros tecidos e células. Diversos estudos relacionam a melatonina a funções imunomodulatórias, de… (more)

▼ A melatonina, principal hormônio produzido pela pineal, pode também ser produzida por outros tecidos e células. Diversos estudos relacionam a melatonina a funções imunomodulatórias, de forma dose-dependente. Considerando sua ação antioxidante e antiinflamatória, avaliou-se o efeito protetor da melatonina na endotoxemia grave, induzida pela administração intraperitoneal de LPS (10 mg/kg) em camundongos. O tratamento com a melatonina (10 e 20 mg/kg, via subcutânea) foi realizado 30 min antes e 1h após o LPS. A endotoxina causou 80% de mortalidade após uma semana, e a melatonina aumentou a sobrevida para 80%, por até 7 dias para o grupo tratado com a menor dose. Para identificar mecanismos envolvidos no efeito protetor da melatonina, foram avaliados parâmetros inflamatórios após a inflamação peritoneal induzida por tioglicolato. Os resultados demonstraram efeito diferencial da melatonina sobre a infiltração de neutrófilos, dependendo do período de observação. Em 6 h houve intensa redução no número de neutrófilos emigrados para o sítio inflamatório, nos animais com endotoxemia, comparado ao controle, e também nos grupos da melatonina. Porém, a dose de 10 mg/kg aumentou parcialmente (p=0,052), a migração de neutrófilos comparado ao LPS. Já após 24 h esta migração não apresentou diferenças entre os grupos controle e LPS. A comparação do grupo do LPS mostra recuperação da capacidade de migração de neutrófilos após 24 h da endotoxemia. Ainda, neste tempo a melatonina inibiu a migração de neutrófilos estimulada pelo tioglicolato, comparado ao controle e ao LPS. Considerando que o efeito inibitório do LPS sobre a migração de neutrófilos pode ser mediado pelo NO, os metabólitos de NO foram quantificados no soro e lavado peritoneal, através da reação de Griess. Houve aumento nas concentrações de NO no soro após 6 e 24 h, no grupo do LPS comparado ao controle, reduzido significativamente pela melatonina (10 mg/kg) após 24 h. No lavado peritoneal não foram detectadas diferenças significativas na produção de nitrito entre os grupos controle e LPS. A melatonina, na dose de 20 mg/kg e após 24 h, reduziu a concentração local de nitrito, comparado ao LPS. Houve redução desta produção ao longo dos tempos de avaliação, significativa para o grupo da melatonina 20 mg/kg. A atividade antioxidante local e sistêmica foi determinada através do teste do DPPH. Não houve diferença significativa entre os grupos quanto à atividade presente no soro nos tempos avaliados. Observou-se redução da atividade antioxidante sistêmica em todos os grupos após 24h da endotoxemia. A atividade antioxidante no lavado peritoneal aumentou significativamente no grupo do LPS, comparado ao PBS, após 24 h, sem diferenças nos grupos da melatonina. Observou-se aumento da atividade antioxidante local em todos os grupos após 24 h. Nossos resultados demonstraram aumentotanto da resposta pró-inflamatória, quanto da anti-inflamatória nos animais administrados com a endotoxina. A melatonina nas duas doses utilizadas, reduziu significativamente as concentrações sistêmicas de… Advisors/Committee Members: Beatriz Martins Tavares Murta.

Subjects/Keywords: Atividade antioxidante; Endotoxemia; MEDICINA; Melatonina; Migração de neutrófilos; Óxido nítrico

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APA (6^th Edition):

Seabra, A. M. d. S. (2012). Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia. (Thesis). Universidade Federal do Triângulo Mineiro. Retrieved from http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=186 ; http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=187

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Seabra, Aletéia Marieta da Silva. “Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia.” 2012. Thesis, Universidade Federal do Triângulo Mineiro. Accessed January 22, 2021. http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=186 ; http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=187.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Seabra, Aletéia Marieta da Silva. “Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia.” 2012. Web. 22 Jan 2021.

Vancouver:

Seabra AMdS. Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia. [Internet] [Thesis]. Universidade Federal do Triângulo Mineiro; 2012. [cited 2021 Jan 22]. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=186 ; http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=187.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Seabra AMdS. Efeito da melatonina sobre a migração de leucócitos, produção de óxido nítrico, citocinas e substâncias oxidantes na endotoxemia. [Thesis]. Universidade Federal do Triângulo Mineiro; 2012. Available from: http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=186 ; http://bdtd.uftm.edu.br/tde_busca/arquivo.php?codArquivo=187

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Aida Mehanna. Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico.

Degree: 2008, Universidade Estadual de Londrina

URL: http://www.bibliotecadigital.uel.br/document/?code=vtls000127659

► A sepse é caracterizada por hipotensão sistêmica, hiporreatividade aos vasoconstritores, perfusão tecidual inadequada e falência múltipla de órgãos. Um dos principais mediadores envolvidos é o… (more)

▼ A sepse é caracterizada por hipotensão sistêmica, hiporreatividade aos vasoconstritores, perfusão tecidual inadequada e falência múltipla de órgãos. Um dos principais mediadores envolvidos é o óxido nítrico (NO). Durante o treinamento físico (TF), ajustes cardiovasculares dinâmicos mantêm a pressão sangüínea adequada e ajustam o suprimento sangüíneo aos diferentes leitos vasculares, e o NO parece estar participando desse processo. O objetivo principal desse estudo foi verificar se o treinamento físico pela natação tem efeito preventivo sobre as anormalidades cardiovasculares e pulmonares induzidas pela administração de lipopolissacárideos de Escherichia coli (LPS), e de que maneira o NO contribui para essas alterações. Para este estudo foram usados ratos Wistar machos (200 a 220 g), submetidos ao treinamento físico pela natação (1 hora; 5 dias/semana; 4 semanas). Os animais sedentários (SED) ou treinados (TF) foram submetidos à cateterização da artéria e veia femorais. Após 24 horas do procedimento cirúrgico, os animais foram tratados com salina 0,9% e com inibidores da NOS: Aminoguanidina (Amino), L-NAME e 7-NI e submetidos à endotoxemia através da administração de LPS (5mg/kg, i.v.). Os parâmetros cardiovasculares foram analisados por 2 horas, e os animais foram sacrificados 5 horas após a administração de LPS, para a quantificação do edema pulmonar. Os parâmetros basais da pressão arterial média (PAM), não mostraram diferenças entre os grupos, no entanto na freqüência cardíaca basal (FC), foi observado bradicardia de repouso nos animais treinados. Neste estudo, nós também investigamos o envolvimento dos inibidores das vias indutiva e constitutiva da NOS nas respostas cardiovasculares em animais sedentários e treinados sem endotoxemia por LPS. Nossos dados mostraram uma resposta pressora tônica nos grupos sedentários e treinados tratados com L-NAME (inibidor da cNOS), onde houve um aumento nos valores da pressão arterial média em relação ao basal, acompanhados de bradicardia, sendo que no grupo treinado a administração de L-NAME, levou a uma menor resposta hipertensiva, quando comparado ao grupo sedentário. Após a administração de LPS foi observada hipotensão e taquicardia no grupo SED tratado com salina, sendo que estas alterações foram atenuadas no grupo TF. No entanto, após a Amino no grupo SED a hipotensão foi atenuada, com aumento na taquicardia. Após o L-NAME no grupo SED a hipotensão foi acentuada, com atenuação da taquicardia. Após a Amino no grupo TF a hipotensão foi acentuada, com atenuação da taquicardia. O L-NAME no grupo TF bloqueou a hipotensão e atenuou a taquicardia. Os animais treinados mostraram um menor índice de edema após o LPS, o qual foi atenuado em ambos os grupos após o tratamento com Amino. Após o tratamento com L-NAME, houve um aumento do índice de edema pulmonar tanto no grupo SED quanto no grupo TF. O tratamento com 7-NI não mostrou qualquer alteração em ambos os grupos. A dosagem de TNF-a no plasma dos animais revelou que o treinamento físico diminui os níveis dessa citocina e que o… Advisors/Committee Members: Marli Cardoso Martins Pinge ., Hugo Celso Dutra, Rubens Cecchini.

Subjects/Keywords: Patologia experimental; Endotoxemia; Óxido nítrico; Experimental pathology; Nitric oxide

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APA (6^th Edition):

Mehanna, A. (2008). Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico. (Thesis). Universidade Estadual de Londrina. Retrieved from http://www.bibliotecadigital.uel.br/document/?code=vtls000127659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mehanna, Aida. “Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico.” 2008. Thesis, Universidade Estadual de Londrina. Accessed January 22, 2021. http://www.bibliotecadigital.uel.br/document/?code=vtls000127659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mehanna, Aida. “Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico.” 2008. Web. 22 Jan 2021.

Vancouver:

Mehanna A. Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico. [Internet] [Thesis]. Universidade Estadual de Londrina; 2008. [cited 2021 Jan 22]. Available from: http://www.bibliotecadigital.uel.br/document/?code=vtls000127659.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mehanna A. Efeitos fisiopatológicos da endotoxemia por LPS em ratos acordados e submetidos ao treinamento físico : papel do óxido nítrico. [Thesis]. Universidade Estadual de Londrina; 2008. Available from: http://www.bibliotecadigital.uel.br/document/?code=vtls000127659

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

21. Richter, Chesney Kirstin. Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors.

Degree: 2016, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/29558

► Nutritional interventions can be used to modify multiple risk factors for cardiovascular disease (CVD). This dissertation research investigated the effects of plant-derived bioactives and long-chain… (more)

▼ Nutritional interventions can be used to modify multiple risk factors for cardiovascular disease (CVD). This dissertation research investigated the effects of plant-derived bioactives and long-chain omega-3 fatty acids on CVD risk factors. The first study evaluated whether incorporating 40 g of freeze dried strawberry powder into a high-fat meal altered postprandial vascular function or attenuated triglyceride, glucose, and insulin responses. Overweight but otherwise healthy adults (n = 30) consumed a control and strawberry meal in a randomized crossover design. Both meals significant decreased the augmentation index (p < 0.002) and significant increased serum triglycerides, insulin, and glucose compared to baseline (p < 0.001), but with no significant differences between meals. Longer-term strawberry supplementation may be needed to improve CVD risk factors. The second study was a placebo-controlled, double-blind, randomized, crossover trial (8 wk treatment, 8 wk washout) investigating the effects of a pharmaceutical dose (3.4 g/d) of EPA+DHA on inflammatory responses to low-dose endotoxemia (intravenous 0.6 ng/kg purified lipopolysaccharide) in 20 healthy young men. Endotoxin administration produced a robust inflammatory response with consistent elevations in C-reactive protein, interleukin-6, and tumor necrosis factor-α; however, there was no difference in the peak concentration of any inflammatory markers following EPA+DHA supplementation compared to the olive oil control (p > 0.05). Omega-3 fatty acids may exert beneficial effects on inflammation via mechanisms other than cytokine production. The third study analyzed the effect of EPA+DHA supplementation on urinary isoprostane production following low-dose endotoxemia in two study populations: 1) described above and 2) healthy young adults (n = 17 [10M]) supplemented with 0, 300, 600, 900, or 1800 mg/d EPA+DHA for 5 months. In both studies, EPA+DHA increased F3-isoprostanes. In the study of healthy adults, EPA+DHA supplementation (600-1800 mg/d) attenuated total urinary isoprostane production in a model that also included body mass index. More research is needed to clarify whether omega-3s can attenuate acute oxidative stress. Although neither the strawberry nor the omega-3 intervention demonstrated a significant effect on the specified CVD risk factors, these null findings improve our understanding of these interventions and will aid in the development of future clinical studies of dietary bioactives. Advisors/Committee Members: Penny Margaret Kris Etherton, Dissertation Advisor/Co-Advisor, Penny Margaret Kris Etherton, Committee Chair/Co-Chair, Gordon Lee Jensen, Committee Member, Sheila Grace West, Committee Member, Eric Loken, Committee Member.

Subjects/Keywords: inflammation; fish oil; endotoxemia; strawberries; oxidative stress; arterial stiffness; postprandial dysmetabolism

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APA (6^th Edition):

Richter, C. K. (2016). Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/29558

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Richter, Chesney Kirstin. “Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors.” 2016. Thesis, Penn State University. Accessed January 22, 2021. https://submit-etda.libraries.psu.edu/catalog/29558.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Richter, Chesney Kirstin. “Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors.” 2016. Web. 22 Jan 2021.

Vancouver:

Richter CK. Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors. [Internet] [Thesis]. Penn State University; 2016. [cited 2021 Jan 22]. Available from: https://submit-etda.libraries.psu.edu/catalog/29558.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Richter CK. Plant-derived bioactives and long-chain omega-3 fatty acids as nutritional interventions for cardiovascular disease risk factors. [Thesis]. Penn State University; 2016. Available from: https://submit-etda.libraries.psu.edu/catalog/29558

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

22. Wong, Hoi Ting. Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.

Degree: 2015, University of Toronto

URL: http://hdl.handle.net/1807/70727

►

Being one of the leading causes of death globally and especially in intensive care units, sepsis is a medical condition of overwhelming inflammatory response due… (more)

Subjects/Keywords: Bead Chemistry; Endotoxemia; Endotoxin; Lipopolysaccharide (LPS); Polymyxin B (PMB); Sepsis; 0485

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APA (6^th Edition):

Wong, H. T. (2015). Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70727

Chicago Manual of Style (16^th Edition):

Wong, Hoi Ting. “Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.” 2015. Masters Thesis, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/70727.

MLA Handbook (7^th Edition):

Wong, Hoi Ting. “Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood.” 2015. Web. 22 Jan 2021.

Vancouver:

Wong HT. Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. [Internet] [Masters thesis]. University of Toronto; 2015. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/70727.

Council of Science Editors:

Wong HT. Polymyxin B-modified Glass Beads: an Alternative Approach to Reduce Lipopolysaccharide Toxicity in Blood. [Masters Thesis]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/70727

23. Smith, Fiona Susan. The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock.

Degree: PhD, 1998, University of Sussex

URL: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263207

Subjects/Keywords: 572; Sepsis; Endotoxemia; Septic shock

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APA (6^th Edition):

Smith, F. S. (1998). The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock. (Doctoral Dissertation). University of Sussex. Retrieved from https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263207

Chicago Manual of Style (16^th Edition):

Smith, Fiona Susan. “The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock.” 1998. Doctoral Dissertation, University of Sussex. Accessed January 22, 2021. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263207.

MLA Handbook (7^th Edition):

Smith, Fiona Susan. “The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock.” 1998. Web. 22 Jan 2021.

Vancouver:

Smith FS. The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock. [Internet] [Doctoral dissertation]. University of Sussex; 1998. [cited 2021 Jan 22]. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263207.

Council of Science Editors:

Smith FS. The regulation of hepatic nitric oxide synthesis and inhibition of glucose output during endotoxic shock. [Doctoral Dissertation]. University of Sussex; 1998. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263207

24. Bidne, Katie Lee. Investigating the ovarian response to endotoxemia.

Degree: 2017, Iowa State University

URL: https://lib.dr.iastate.edu/etd/16506

► The ovary is responsible for production of steroid hormones and gametes in the female. It is endowed with a finite pool of primordial follicles, established… (more)

▼ The ovary is responsible for production of steroid hormones and gametes in the female. It is endowed with a finite pool of primordial follicles, established in utero, that serve as the source of oocytes during a female’s reproductive lifespan. Alterations to ovarian function and signaling can result in disruptions to steroid hormone production, follicle activation, and oocyte quality. The degree of disruption is dependent on the developmental or estrous cycle stage of the animal as well as the duration of the perturbation. Inflammation can have deleterious effects on ovarian signaling and function, and can arise from multiple sources, such as endotoxemia, or increased levels of lipopolysaccharide (LPS) in blood. One major source of LPS is the intestine, where stresses such as hypoxia due to heat stress, high fat diets, or obesity can induce increased intestinal permeability and allow passage of LPS from the intestinal lumen into circulation. LPS signaling via its receptor, toll-like receptor 4 (TLR4), may induce inflammation in the ovary, potentially compromising oocyte and follicular quality, resulting in reduced fertility. This thesis investigated the central hypothesis that increases in circulating LPS would alter ovarian signaling and function as evidenced by activation of the TLR4, phosphatidylinositol 3 kinase (PI3K), and steroidogenic pathways. To test this hypothesis, we utilized three animal model-LPS exposure schemes in pigs: post-pubertal chronic LPS infusion, post-pubertal heat stress (HS), and pre-pubertal high fat diet (HFD). We analyzed ovarian protein and mRNA abundance via western blotting and qRT-PCR, as well as quantification of 17β-estradiol, progesterone, and LPS binding protein concentrations via ELISAs. Overall, our findings demonstrate that the ovary is responsive to LPS as demonstrated by increased ovarian TLR4 in an LPS infusion but not HFD model. We also discovered alterations to the PI3K and steroidogenic pathways in our HFD model not seen with the other exposure paradigms, indicating a potential effect of developmental age or an alternate effector mechanism in these pigs. Taken together, these data demonstrate the alterations in ovarian function due to different exposures to LPS in isolation or combination with other physiological changes. This understanding is vital to the development of amelioration strategies to reduce infertility.

Subjects/Keywords: endotoxemia; heat stress; high fat diet; lipopolysaccharide; ovary; Agriculture; Animal Sciences

10 more images…

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APA (6^th Edition):

Bidne, K. L. (2017). Investigating the ovarian response to endotoxemia. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16506

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bidne, Katie Lee. “Investigating the ovarian response to endotoxemia.” 2017. Thesis, Iowa State University. Accessed January 22, 2021. https://lib.dr.iastate.edu/etd/16506.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bidne, Katie Lee. “Investigating the ovarian response to endotoxemia.” 2017. Web. 22 Jan 2021.

Vancouver:

Bidne KL. Investigating the ovarian response to endotoxemia. [Internet] [Thesis]. Iowa State University; 2017. [cited 2021 Jan 22]. Available from: https://lib.dr.iastate.edu/etd/16506.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bidne KL. Investigating the ovarian response to endotoxemia. [Thesis]. Iowa State University; 2017. Available from: https://lib.dr.iastate.edu/etd/16506

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

25. Aboubaker, Eltayb Abdelwahab Mohamed.; University of Aberdeen.Dept. of Zoology. Trypanosomiasis.

Degree: Dept. of Zoology., 2017, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=231772 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=231772&custom_att_2=simple_viewer

Subjects/Keywords: Trypanosoma.; Trypanosoma brucei.; Trypanosomiasis; Endotoxemia.

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APA (6^th Edition):

Aboubaker, E. A. M. ;. U. o. A. D. o. Z. (2017). Trypanosomiasis. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=231772 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=231772&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed ; University of Aberdeen Dept of Zoology. “Trypanosomiasis.” 2017. Doctoral Dissertation, University of Aberdeen. Accessed January 22, 2021. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=231772 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=231772&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Aboubaker, Eltayb Abdelwahab Mohamed ; University of Aberdeen Dept of Zoology. “Trypanosomiasis.” 2017. Web. 22 Jan 2021.

Vancouver:

Aboubaker EAM;UoADoZ. Trypanosomiasis. [Internet] [Doctoral dissertation]. University of Aberdeen; 2017. [cited 2021 Jan 22]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=231772 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=231772&custom_att_2=simple_viewer.

Council of Science Editors:

Aboubaker EAM;UoADoZ. Trypanosomiasis. [Doctoral Dissertation]. University of Aberdeen; 2017. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=231772 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=231772&custom_att_2=simple_viewer

Virginia Tech

26. Chang, Samantha Mee. A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia.

Degree: PhD, Veterinary Medical Sciences, 2011, Virginia Tech

URL: http://hdl.handle.net/10919/77155

► Lipopolysaccharide (LPS), a Gram-negative endotoxin, has been well-established as the trigger for the effects of sepsis and septic shock through its binding with the innate… (more)

▼ Lipopolysaccharide (LPS), a Gram-negative endotoxin, has been well-established as the trigger for the effects of sepsis and septic shock through its binding with the innate immune receptor, Toll-like receptor 4 (TLR4). High doses of LPS signal through TLR4 to produce a massive release of pro-inflammatory cytokines including IL-6, TNFα, and other. Additionally, several recent publications have demonstrated severe metabolic alterations after LPS challenge, suppressing lipid oxidation and concurrently up-regulating glucose oxidation. Unfortunately, this switch in metabolism is inefficient for the great energy demands of the host during a systemic microbial infection which can result in vital organ failure. Meanwhile, a novel concept in several chronic disease pathologies also implicates LPS, although at very low doses. The presence of subclinically elevated circulating endotoxin levels has been termed metabolic endotoxemia and is beginning to be investigated in disease pathologies including insulin resistance and type II diabetes, atherosclerosis, cancer metastasis and Parkinson's disease. These disease phenotypes all possess a component of chronic inflammation whose source has not largely been understood, but examining the effects of very low doses of LPS may provide vital information in understanding their etiologies. However, most information on LPS signaling has been obtained using high doses of LPS (10-200ng/ml) while little to no studies have been published regarding the effects of very low doses of LPS (1pg-100pg/ml) on inflammatory and metabolic alterations. Thus, we use in vivo and in vitro models to determine that both IRAK1 and JNK are critical points of crosstalk downstream of TLR4 for the metabolic and inflammatory alterations associated with metabolic endotoxemia. Additionally, we observed significant down-regulation of nuclear receptors responsible for fatty acid metabolism, including PGC1α, PPARα, and PPARγ after very low dose LPS challenge. Further, we observe phenotypic changes in fatty acid oxidation and glucose oxidation, as well as subsequent changes in cytosolic acetyl-CoA levels and acetylation of pro-inflammatory transcription factor ATF2. Overall our studies point to several mechanisms of cross-talk between metabolism and inflammation and offer significant support to the concept of metabolic endotoxemia in the development of chronic disease. Advisors/Committee Members: Meng, Xiang-Jin (committee member), LeRoith, Tanya (committee member), Li, Liwu (committeecochair), Ahmed, S. Ansar (committeecochair).

Subjects/Keywords: lipopolysaccharide; chronic inflammatory disease; Toll-like Receptor 4; Metabolic endotoxemia

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APA (6^th Edition):

Chang, S. M. (2011). A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77155

Chicago Manual of Style (16^th Edition):

Chang, Samantha Mee. “A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia.” 2011. Doctoral Dissertation, Virginia Tech. Accessed January 22, 2021. http://hdl.handle.net/10919/77155.

MLA Handbook (7^th Edition):

Chang, Samantha Mee. “A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia.” 2011. Web. 22 Jan 2021.

Vancouver:

Chang SM. A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia. [Internet] [Doctoral dissertation]. Virginia Tech; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10919/77155.

Council of Science Editors:

Chang SM. A Mechanism for the Metabolic and Inflammatory Alterations Associated with Low-dose Endotoxemia. [Doctoral Dissertation]. Virginia Tech; 2011. Available from: http://hdl.handle.net/10919/77155

Virginia Tech

27. Pantaleon, Lucas Guillermo. Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses.

Degree: MS, Veterinary Medical Sciences, 2005, Virginia Tech

URL: http://hdl.handle.net/10919/33885

► Justification: Endotoxemia and sepsis are major causes of mortality in horses, resulting in significant economic losses for the equine industry. Objective: To determine the effects… (more)

Subjects/Keywords: Hypertonic Saline; Hetastarch; horse; endotoxemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pantaleon, L. G. (2005). Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/33885

Chicago Manual of Style (16^th Edition):

Pantaleon, Lucas Guillermo. “Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses.” 2005. Masters Thesis, Virginia Tech. Accessed January 22, 2021. http://hdl.handle.net/10919/33885.

MLA Handbook (7^th Edition):

Pantaleon, Lucas Guillermo. “Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses.” 2005. Web. 22 Jan 2021.

Vancouver:

Pantaleon LG. Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses. [Internet] [Masters thesis]. Virginia Tech; 2005. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10919/33885.

Council of Science Editors:

Pantaleon LG. Cardiovascular and Hematological Effects of Hetastarch and Hypertonic Saline Solutions During Experimental Endotoxemia in Horses. [Masters Thesis]. Virginia Tech; 2005. Available from: http://hdl.handle.net/10919/33885

Virginia Tech

28. MacDonald, Elizabeth Steward. The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin.

Degree: MS, Biomedical and Veterinary Sciences, 2015, Virginia Tech

URL: http://hdl.handle.net/10919/76722

► Endotoxemia is a major cause of morbidity and mortality in horses. The presence of large amounts of circulating endotoxin inititates a number of cell signaling… (more)

▼ Endotoxemia is a major cause of morbidity and mortality in horses. The presence of large amounts of circulating endotoxin inititates a number of cell signaling pathways leading to a systemic inflammatory response. Activation of these pathways causes the release of a number of pro- and anti-inflammatory mediators. An overwhelming release of these mediators leads to the development of clinical signs associated with endotoxemia. Treatment options are limited mostly to supportive care at this time. Mesenchymal stem cells (MSCs) have been shown to have anti-inflamamtory and immune modulatory effects that may have some benefit for the treatment of horses with endotoxemia. To evaluate the effect of equine MSCs on the response to endotoxin challenge, the study was performed on two different stem cell lines with peripheral blood mononuclear cells (PBMCs) used as controls. After stimulation with endotoxin, secretion of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), and interferon gamma (IFN-γ) were determined by ELISA. The immunogenic properties of MSCs were assessed with a one-way mixed lymphocyte reaction. In addition, the ability of MSCs to alter production of cytokines from stimulated PBMCs was assessed. TNF-α was not produced by MSCs when compared to PBMCs (p = < 0.001). There was no significant difference between MSCs and PBMCs in the production of IL-6. IL-10 production was significantly different (p = <0.001) at 6 and 12 hours with MSCs producing more than PBMCs in one stem cell line only. MSCs did not stimulate proliferation of PBMCs. Co-incubation of MSCs with PBMCs decreased the production of TNF-α in both stem cell lines although it was not statistically significant (p = 0.4 and 0.9) at either time point. IL-6 secretion was suppressed at twelve hours with co-incubation. IL-10 production was increased with co-incubation in one stem cell line. MSCs secrete soluble factors that can alter PBMC cytokine production and they do not appear to be immunostimulatory. These findings have potential implication for treatment of equine inflammatory conditions. Advisors/Committee Members: Furr, Martin O. (committeechair), McKenzie, Harold C. (committee member), Barrett, Jennifer G. (committee member).

Subjects/Keywords: equine; endotoxemia; bone marrow derived mesenchymal stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

MacDonald, E. S. (2015). The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/76722

Chicago Manual of Style (16^th Edition):

MacDonald, Elizabeth Steward. “The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin.” 2015. Masters Thesis, Virginia Tech. Accessed January 22, 2021. http://hdl.handle.net/10919/76722.

MLA Handbook (7^th Edition):

MacDonald, Elizabeth Steward. “The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin.” 2015. Web. 22 Jan 2021.

Vancouver:

MacDonald ES. The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin. [Internet] [Masters thesis]. Virginia Tech; 2015. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10919/76722.

Council of Science Editors:

MacDonald ES. The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin. [Masters Thesis]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/76722

Virginia Tech

29. Price, Kathryn Leigh. Plasma amino acid and metabolite changes in pigs during endotoxemia.

Degree: PhD, Animal and Poultry Sciences, 2011, Virginia Tech

URL: http://hdl.handle.net/10919/77279

► The nutritional status, especially circulating amino acid (AA) levels, can drastically change during a non-infectious (i.e., LPS) or infectious (e.g., Salmonella) challenge. Thus, study 1… (more)

▼ The nutritional status, especially circulating amino acid (AA) levels, can drastically change during a non-infectious (i.e., LPS) or infectious (e.g., Salmonella) challenge. Thus, study 1 examined the effect of LPS treatment (N = 9, 26.9 ± 1.07 kg BW) on plasma AA and metabolite levels in pigs. Data were used to generate prediction equations establishing mathematical relationships between plasma AA levels and numerous blood metabolites (e.g., total lipid, LDL, HDL, blood urea nitrogen, etc). These equations have the potential to improve the nutritional treatment and recovery of acute and chronically ill patients. Study 2 (19.1 ± 0.37 kg) was a continuation of study 1 except the sampling time was increased from 12 to 24 h. One-half of the pigs in study 2 were treated with LPS (N=15) and the other one-half were saline treated control animals (N = 16). This design allows for monitoring changes in plasma AA and their catabolism in response to endotoxemia. Area under the curve (AUC) was calculated for a selected AA to report AA balances. During the induction phase of an acute challenge (t = -2 to 12 h), analyzed AA were in a negative balance indicating heavy AA catabolism. However, during the recovery phase (t = 12 to 24 h) half of the AA were in a positive balance while the other half were still negative. The ability of equations to accurately predict AA concentrations was tested. Results indicate poor performance possibly due to heavy term biases. Thus, it was concluded that equations need to be revisited and non-linear terms need to be evaluated. Nonetheless, routine clinical blood metabolites can be used to estimate plasma AA levels during immune activation. We successfully established a porcine Salmonella enterica serovar Typhimurium model. Pigs infected with Salmonella had a febrile response for 4 d and exhibited marked changes in their fecal bacterial populations Finally, we investigated plasma changes in N-τ-methyl histidine (NτMH) in healthy and LPS-treated pigs. NτMH— is a post-translationally modified AA that has historically been used as an indirect marker of muscle protein breakdown in rodents and humans. However, the major form (i.e., free or acetylated) of NτMH in pig plasma was unknown. Results indicate that only 15% of plasma NτMH is in the free form and the remainder is acetylated. Furthermore, LPS treated pigs had increased acetylated and total NτMH fractions while free NτMH did not change. Therefore, to accurately monitor plasma changes in NτMH as an indicator of muscle proteolysis, plasma samples must be subjected to acid hydrolysis. Advisors/Committee Members: Escobar, Jeffery E. (committeechair), Hanigan, Mark D. (committee member), Harper, Allen F. (committee member), Pelzer, Kevin D. (committee member).

Subjects/Keywords: NτMH; Salmonella; endotoxemia; pigs; blood metabolites; amino acids

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Price, K. L. (2011). Plasma amino acid and metabolite changes in pigs during endotoxemia. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77279

Chicago Manual of Style (16^th Edition):

Price, Kathryn Leigh. “Plasma amino acid and metabolite changes in pigs during endotoxemia.” 2011. Doctoral Dissertation, Virginia Tech. Accessed January 22, 2021. http://hdl.handle.net/10919/77279.

MLA Handbook (7^th Edition):

Price, Kathryn Leigh. “Plasma amino acid and metabolite changes in pigs during endotoxemia.” 2011. Web. 22 Jan 2021.

Vancouver:

Price KL. Plasma amino acid and metabolite changes in pigs during endotoxemia. [Internet] [Doctoral dissertation]. Virginia Tech; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10919/77279.

Council of Science Editors:

Price KL. Plasma amino acid and metabolite changes in pigs during endotoxemia. [Doctoral Dissertation]. Virginia Tech; 2011. Available from: http://hdl.handle.net/10919/77279

30. Κampisiouli, Efstathia. Γκρελίνη και σήψη.

Degree: 2019, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/46742

►

Background: Ghrelin is a hormone mainly produced by cells of the gastric mucosa, which has been shown to possess anti-inflammatory and immunomodulatory properties. The objective… (more)

▼

Background: Ghrelin is a hormone mainly produced by cells of the gastric mucosa, which has been shown to possess anti-inflammatory and immunomodulatory properties. The objective of the study was to investigate ghrelin levels during sepsis, as well as in an experimental sepsis model. Methods: All consecutive admissions to the ICU of a tertiary hospital in Athens, Greece were screened for eligibility during the study. Thirty four non-septic patients upon ICU admission who subsequently developed sepsis were enrolled. Clinical data and scores were recorded, and blood samples were obtained at baseline (upon ICU admission), and at sepsis development. Total and active ghrelin, leptin, and cytokines were measured. Moreover, lipopolysaccharide (LPS) was administered to mice in order to induce endotoxemia and at specified time points, blood and tissue samples were collected. Results: In patients, serum total and active ghrelin concentrations were significantly elevated in sepsis compared to baseline (553.8±213.4 vs 193.5±123.2, p<0.001; 254.3±70.6 vs 56.49±16.3, p<0.001). Active ghrelin levels at the sepsis stage were inversely correlated with SOFA score and length of stay in the ICU (p=0.023 and p=0.027 respectively). In the mouse endotoxemia model ghrelin levels were elevated following LPS treatment, and the same trend was observed for leptin, TNF-α and IL-6. Ghrelin administration managed to reduce IL-6 levels in mouse serum and in BALF. Pulmonary expression of ghrelin and its receptor GHSR1a was found decreased in LPS-treated mice. Conclusions: In a well-defined cohort of ICU patients, we have demonstrated that active and total ghrelin increase in sepsis. The same is true for the experimental sepsis model used in the study. The inverse correlation of active ghrelin levels with SOFA score and length of ICU stay among septic patients is indicative of a potential protective role of active ghrelin during the septic process.

Εισαγωγή: Η γκρελίνη είναι μια ορμόνη που παράγεται κυρίως από κύτταρα του γαστρικού βλεννογόνου, η οποία έχει δειχθεί ότι έχει αντιφλεγμονώδεις και ανοσοδιαμορφωτικές ιδιότητες. Σκοπός: Η διερεύνηση των επιπέδων της γκρελίνης στη φάση της σήψης σε βαρέως πάσχοντες ασθενείς, καθώς και σε πειραματικό μοντέλο σήψης. Υλικό-Μέθοδος: Όλες οι διαδοχικές εισαγωγές, σε τριτοβάθμιο νοσοκομείο της Αθήνας, κατά τη διάρκεια της μελέτης ελέγχθηκαν για την επιλεξιμότητα τους. Τριάντα τέσσερις αρχικά μη σηπτικοί βαρέως πάσχοντες ασθενείς συμπεριελήφθησαν τελικά στη μελέτη. Καταγράφηκαν τα κλινικό-εργαστηριακά δεδομένα και οι κλίμακες βαρύτητας ενώ δείγματα αίματος ελήφθησαν κατά την εισαγωγή στην ΜΕΘ και στην ημέρα εμφάνισης σήψης. Αξιολογήθηκαν τα επίπεδα ολικής και δραστικής γκρελίνης, λεπτίνης και κυτταροκινών. Επιπλέον, χορηγήθηκε λιποπολυσακχαρίτης (LPS) σε ποντικούς προκειμένου να προκληθεί ενδοτοξιναιμία και σε συγκεκριμένα χρονικά σημεία συλλέχθηκαν δείγματα αίματος , βρογχοκυψελιδικού εκπλύματος (BALF) και ιστών. Αποτελέσματα: Στους ασθενείς, τα επίπεδα ολικής και δραστικής γκρελίνης στον ορό βρέθηκαν…

Subjects/Keywords: Γκρελίνη; Σήψη; ΜΕΘ; Ενδοτοξιναιμία; Ghrelin; Sepsis; ICU; Endotoxemia

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Κampisiouli, E. (2019). Γκρελίνη και σήψη. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/46742

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Κampisiouli, Efstathia. “Γκρελίνη και σήψη.” 2019. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed January 22, 2021. http://hdl.handle.net/10442/hedi/46742.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Κampisiouli, Efstathia. “Γκρελίνη και σήψη.” 2019. Web. 22 Jan 2021.

Vancouver:

Κampisiouli E. Γκρελίνη και σήψη. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10442/hedi/46742.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Κampisiouli E. Γκρελίνη και σήψη. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. Available from: http://hdl.handle.net/10442/hedi/46742

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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