subject:(embryo transfer)

University of Georgia

1. Ray, Lucy Eleanor. A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/24256

► Vitrification of equine embryos shows fairly consistent success rates when they are frozen at d 300µm or about 6.5 d after ovulation. In this study,… (more)

▼ Vitrification of equine embryos shows fairly consistent success rates when they are frozen at d 300µm or about 6.5 d after ovulation. In this study, the effects of the reduction of blastocoelic fluid and the microinfusion of a cryoprotectant prior to vitrification were examined on 8 d embryos. The equine embryos used for this project were 805µm (1), 820µm (2), 1120µm (3), 1286µm (4), and 979µm (5). All embryos were graded excellent (1) according to IETS guidelines. The embryos were either assigned to a control group in which no micromanipulation occurred (1-3) or to the experimental group which entailed microinfusion of VS1 (1.4 M glycerol in PBS; 4-5) after aspiration of blastocoelic fluid before microinfusion of VS1 (5). During the following vitrification procedure, the embryos were exposed to VS1 and 2 (1.4M glycerol, 3.6M ethylene glycol in PBS) for 5 minutes, VS3 (3.4M glycerol, 6.6M ethylene glycol in PBS) for 1 minute. The embryo in VS3 was then loaded into a 0.25 ml straw, separated by two air bubbles from columns of 0.5 M galactose. The straws were placed in a cooled plastic goblet surrounded by liquid nitrogen vapors for 1 minute and then finally immersed into the liquid nitrogen. Digital pictures were taken throughout the process. The equine embryos failed to yield a pregnancy with the exception of one (5) which formed an embryonic vesicle at d 15 after ovulation. Ultrasonography at d 28 revealed resorption of the embryo, probably due to heat stress. Due to the low number of equine embryos recovered, porcine embryos were used as a model in another trial. Vitrification of 44 porcine embryos was attempted using identical procedures to those described above. The porcine embryos were cultured for a period of 24 hrs and digital images were also taken. Twenty-six embryos were frozen using the control method and 20 were assigned to the experimental group and treated as described earlier. Initial reexpansion was complete in 16 of the treated and 7 of the control embryos. This showed a significant difference in treatment and control at thaw (p<.0001). All embryos were completely dead at 24 hrs.

Subjects/Keywords: embryo transfer; vitrification; cryopreservation; equine embryo; porcine embryo

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APA (6^th Edition):

Ray, L. E. (2014). A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/24256

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ray, Lucy Eleanor. “A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation.” 2014. Thesis, University of Georgia. Accessed February 27, 2021. http://hdl.handle.net/10724/24256.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ray, Lucy Eleanor. “A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation.” 2014. Web. 27 Feb 2021.

Vancouver:

Ray LE. A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10724/24256.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ray LE. A novel approach to the cryopreservation of equine and porcine embryos by vitrification after blastocoelic micromanipulation. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/24256

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

AUT University

2. Erikson, Elizabeth Kate Louise. In Vivo implantation Murine Modeling .

Degree: 2012, AUT University

URL: http://hdl.handle.net/10292/4734

► Ethical and legal restrictions limit human implantation knowledge. Alternative tools, such as retrospective clinical studies, in vitro three dimensional cell culture modelling, and in vivo… (more)

▼ Ethical and legal restrictions limit human implantation knowledge. Alternative tools, such as retrospective clinical studies, in vitro three dimensional cell culture modelling, and in vivo implantation animal modelling provide some insight. While in vivo implantation murine models have proven useful, none are recognised as standard. Therefore, the overall research objective was to gain an understanding of improved standardization of in vivo implantation murine models, suitable to explore implantation and preliminary studies surrounding In Vitro Fertilization (IVF) intervention potential. A selection of models, termed optimal or suboptimal by their level of implantation, were examined and compared to natural mouse pregnancy. Embryo transfer (ET) outcomes on day 17 post coitus (pc) and the ability of the most suitable optimal and suboptimal models to detect consequences of manipulated embryos, an IVF intervention, were explored. Significant inconsistencies within suboptimal models led to investigating the beginning of endometrial receptivity within natural pregnancy, and synchronous and asynchronous uterine ET. Assessed time points were set to capture the pre receptive and receptive endometrial phases. Examining at least three mice per time point, achieving 80% statistical power, ensured statistical robustness. This research extended current knowledge in three ways. Firstly, ETs performed at two hourly intervals, demonstrated significant influence of the distinct moments of coitus, ET and autopsy on implantation rate consistency. Previous research, which specified the day of transfer rather than hours pc, was unable to reveal such influence. Finer control of coitus, ET and autopsy demonstrated improved implantation rate consistency, necessary for model standardization. Implementation within wider research could improve statistical robustness and facilitate greater comparison between research groups. Secondly, assessed time points extended beyond previous the literature, which did not report endometrial receptivity within natural pregnancy prior to 92 hours pc. This study reported endometrial receptivity by 88 hours pc. No mean zero baselines were also observed, suggesting endometrial receptivity could begin prior to 88 hours pc. A four hour difference is significant, given murine endometrial receptivity is considered at least 12, but less than 24 hours long, translating to a potential 33% increase in known implantation opportunity. Data collection surrounding the beginning of endometrial receptivity within natural pregnancy, and synchronous and asynchronous uterine ET, revealed reasons for implantation rate inconsistencies, not previously observed in single-point data sets; contributing towards the first point. Thirdly, this is the first report of early endometrial receptivity, following the transfer of mature pre-implantation embryos directly into uteri, whose endometrium would not normally be exposed to embryos at that time. Only one other study, which performed oviductal transfers,… Advisors/Committee Members: Henry, Stephen (advisor), Blake, Debbie (advisor).

Subjects/Keywords: Implantation; Mouse; Iin vivo; Modeling; Mice; Murine; Embryo transfer; Uterus; Embryo

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APA (6^th Edition):

Erikson, E. K. L. (2012). In Vivo implantation Murine Modeling . (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/4734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Erikson, Elizabeth Kate Louise. “In Vivo implantation Murine Modeling .” 2012. Thesis, AUT University. Accessed February 27, 2021. http://hdl.handle.net/10292/4734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Erikson, Elizabeth Kate Louise. “In Vivo implantation Murine Modeling .” 2012. Web. 27 Feb 2021.

Vancouver:

Erikson EKL. In Vivo implantation Murine Modeling . [Internet] [Thesis]. AUT University; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10292/4734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Erikson EKL. In Vivo implantation Murine Modeling . [Thesis]. AUT University; 2012. Available from: http://hdl.handle.net/10292/4734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

3. Chiles, Kelley. Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer.

Degree: MS, Physiology of Reproduction, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11197

► Superovulation of donor cows, embryo transfer, and estrus synchronization of recipients are widely used technologies in the purebred cattle industry. Progress continues to be made… (more)

▼ Superovulation of donor cows, embryo transfer, and estrus synchronization of recipients are widely used technologies in the purebred cattle industry. Progress continues to be made to achieve efficient and economic use of these technologies. The first retrospective study was conducted to compare embryo production between a stimulation protocol using only Folltropin as the gonadotropin, and a stimulation protocol using Folltropin and Pluset. Beefmaster donor cows (n=12) were stimulated using both protocols over two stimulated cycles, one protocol each cycle. Both protocols used the same synchronization protocol with only the gonadatropin injections differing. The control protocol (Folltropin protocol) consisted of seven Folltropin injections over the course of 3.5 days. The treatment protocol (Folltropin + Pluset protocol) consisted of four Folltropin injections followed by three Pluset injections over the course of 3.5 days. The mean numbers of viable embryos did not differ between treatments (P>0.01) and were 9.33 and 6.58 for the control and treatment protocols, respectively. The proportion of viable embryos to total ova for each protocol was 0.49 and 0.48 for the control and treatment protocols, respectively (P > 0.10). No significant difference on embryo production was observed between the control and treatment protocols. The second retrospective study was performed to compare pregnancy rates after embryo transfer between Beefmaster recipients who received eCG during synchronization and recipients who did not receive eCG during synchronization. Due to the conditions of this study, statistical analysis could not be performed. Pregnancy rates are reported, but they are not statistically significant. Recipients in the control group (n=332) were synchronized with a protocol using a CIDR insert for seven days, a progesterone estradiol injection at the time of CIDR insertion, a prostaglandin (PG) injection at CIDR removal, and an estradiol injection the day after CIDR removal. Recipients in the treatment group (n=142) were synchronized using the same synchronization protocol as the control group, except eCG was administered five days after CIDR insertion. Pregnancy rates were 44.88 and 38.73 for the control and treatment groups, respectively. The addition of eCG to the synchronization protocol did not appear to be either beneficial or detrimental to pregnancy rate under the conditions of this study. In summary, the addition of Pluset to the stimulation protocol for donors was not detrimental to embryo production. The estrus synchronization protocol with eCG for recipients did not appear to be beneficial; however, a controlled studies are still warranted to further investigate the potential effects of recipient age, parity, body condition score, or breed effect on response to eCG. Advisors/Committee Members: Forrest, David W. (advisor), Amstalden, Marcel (committee member), Long, Charles R. (committee member), Looney, Charles R. (committee member).

Subjects/Keywords: Superovulation; Pluset; Embryo transfer; eCG; Recipients

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chiles, K. (2012). Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11197

Chicago Manual of Style (16^th Edition):

Chiles, Kelley. “Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer.” 2012. Masters Thesis, Texas A&M University. Accessed February 27, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11197.

MLA Handbook (7^th Edition):

Chiles, Kelley. “Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer.” 2012. Web. 27 Feb 2021.

Vancouver:

Chiles K. Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11197.

Council of Science Editors:

Chiles K. Response to Incorporation of Supplemental Gonadotropins for Donor and Recipient Protocols in Commercial Bovine Embryo Transfer. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2012-05-11197

University of Oulu

4. Veleva, Z. (Zdravka). Factors affecting the outcome of IVF/ICSI.

Degree: 2008, University of Oulu

URL: http://urn.fi/urn:isbn:9789514288838

► Abstract Fertility declines with advancing age and the number of couples seeking infertility treatment at an older age is constantly increasing. A top quality embryo… (more)

▼ Abstract Fertility declines with advancing age and the number of couples seeking infertility treatment at an older age is constantly increasing. A top quality embryo is believed to have the highest potential for implantation and development into a child. A better understanding of the relative importance of patient and treatment characteristics and of embryo quality could help to optimise the existing therapeutic schemes and the safety of in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI). In this work, databases of five Finnish infertility clinics were studied retrospectively. Data on treatments performed in the years 1994–2005 were collected. A total of 19,000 treatment cycles were analysed. Special attention was paid to the relative significance of the transfer of top quality embryos with regards to pregnancy, miscarriage, live birth and cost of treatment in the general IVF/ICSI patient population and in groups with expected poor outcome. The results showed that the transfer of a top quality embryo is associated with a better chance of pregnancy and live birth. However, it does not diminish the probability of miscarriage. Both low and high BMI increase the miscarriage rate. Advancing age and a positive history of previous miscarriages are also associated with a higher probability of miscarriage. In addition, the need for hormonal substitution in cases of frozen-embryo transfer is a risk factor of miscarriage, probably because of suboptimal endometrial function. Since the transfer of several embryos leads to multiple pregnancies, which are associated with a high risk of maternal and fetal complications, elective single embryo transfer (eSET) of a top quality embryo allows all additional good quality embryos to be frozen and transferred later in frozen-thawed embryo transfer cycles. The present work demonstrates that eSET is a safe treatment strategy at least until the age of 40. However, it might not be performed in women with fewer than four collected oocytes, since the prognosis might remain poor even if the response is improved in a following cycle. When eSET is applied routinely and on a large scale, it diminishes treatment costs while increasing the number of deliveries occurring at term, making IVF/ICSI at the same time safer and more affordable even to patients without access to reimbursed IVF treatment.

Subjects/Keywords: cost effectiveness; elective single embryo transfer; fertilisation in vitro; frozen-thawed embryo transfer; low response; obesity; spontaneous abortion; top quality embryo

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Veleva, Z. (. (2008). Factors affecting the outcome of IVF/ICSI. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9789514288838

Chicago Manual of Style (16^th Edition):

Veleva, Z (Zdravka). “Factors affecting the outcome of IVF/ICSI.” 2008. Doctoral Dissertation, University of Oulu. Accessed February 27, 2021. http://urn.fi/urn:isbn:9789514288838.

MLA Handbook (7^th Edition):

Veleva, Z (Zdravka). “Factors affecting the outcome of IVF/ICSI.” 2008. Web. 27 Feb 2021.

Vancouver:

Veleva Z(. Factors affecting the outcome of IVF/ICSI. [Internet] [Doctoral dissertation]. University of Oulu; 2008. [cited 2021 Feb 27]. Available from: http://urn.fi/urn:isbn:9789514288838.

Council of Science Editors:

Veleva Z(. Factors affecting the outcome of IVF/ICSI. [Doctoral Dissertation]. University of Oulu; 2008. Available from: http://urn.fi/urn:isbn:9789514288838

5. Maezawa, Tadashi. Possible selection of viable human blastocysts after vitrification by monitoring morphological changes.

Degree: 博士(医学), 2017, Mie University / 三重大学

URL: http://hdl.handle.net/10076/14726

►

Morphological assessment of human blastocysts has been effective for selecting embryos with high potential. However, they often show repeated shrinkage and expansion toward their hatching.… (more)

Subjects/Keywords: Vitrified-warmed blastocyst; Oxygen consumption; Time-lapse images; Embryo selection; Embryo transfer

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APA (6^th Edition):

Maezawa, T. (2017). Possible selection of viable human blastocysts after vitrification by monitoring morphological changes. (Thesis). Mie University / 三重大学. Retrieved from http://hdl.handle.net/10076/14726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Maezawa, Tadashi. “Possible selection of viable human blastocysts after vitrification by monitoring morphological changes.” 2017. Thesis, Mie University / 三重大学. Accessed February 27, 2021. http://hdl.handle.net/10076/14726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Maezawa, Tadashi. “Possible selection of viable human blastocysts after vitrification by monitoring morphological changes.” 2017. Web. 27 Feb 2021.

Vancouver:

Maezawa T. Possible selection of viable human blastocysts after vitrification by monitoring morphological changes. [Internet] [Thesis]. Mie University / 三重大学; 2017. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10076/14726.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Maezawa T. Possible selection of viable human blastocysts after vitrification by monitoring morphological changes. [Thesis]. Mie University / 三重大学; 2017. Available from: http://hdl.handle.net/10076/14726

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

6. Gonzalez Grajales, Leslie Antonio. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.

Degree: PhD, Department of Biomedical Sciences, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

► The success of reproductive biotechnologies including in vitro embryo production in bison species has been low although several techniques, such as interspecies somatic cell nuclear… (more)

▼ The success of reproductive biotechnologies including in vitro embryo production in bison species has been low although several techniques, such as interspecies somatic cell nuclear transfer (iSCNT) hold promise of efficacy in this species. However, interactions between cattle ooplasmic components and bison nuclear structures influence early development in bison iSCNT embryos. Differences in ATP content, incidence of apoptosis, and gene expression between cattle SCNT and bison iSCNT embryos suggest altered interactions in nuclear and mitochondrial genomes, although no differences in developmental rates were observed. A cattle IVM maturation and IVC system were used to determine the developmental potential of plains bison oocytes as cytoplasts. Maturation and developmental rates were similar between cattle and plains bison. The majority of oocytes in both groups reached metaphase meiosis II at 24 hours post maturation and the 8-16 cell stage on day 4. Although total blastocyst rate was similar between cattle and plains bison, embryos in the latter group consistently reached the blastocyst stage 24 to 48 hours after cattle parthenogenic embryos. Modifications to previously described ooplasm transfer techniques were performed to decrease the number of micromanipulations needed when combined with SCNT. The effects of ooplasm transfer into cattle SCNT and iSCNT plains bison embryos was investigated and demonstrated that supplementation of ooplasm matching the genetic origin of the somatic cell in reconstructed SCNT or iSCNT embryos had no effects on development, ATP content, and gene expression profiles at the 8-16 cell stage. Finally, embryo quality between vitrified-thawed wood bison blastocysts produced in vivo and wood bison iSCNT blastocysts generated in vitro was investigated. The percentage of in vivo-derived embryos showing normal morphology was only 20% of that in vitro. Although in vivo embryos expanded, initiated the hatching process, and had higher total cell number, no differences were found in apoptosis incidence when compared to iSCNT embryos. Delayed development at the blastocyst stage was observed in iSCNT wood bison embryos when compared to in vivo embryos. The present study demostrated developmental alterations in iSCNT bison embryos, no effects on development by ooplasm supplementation, and compromised competence of in vivo-derived wood bison embryos. Advisors/Committee Members: King, W. Allan (advisor).

Subjects/Keywords: bison; embryo; interspecies somatic cell nuclear transfer; parthenogenesis; reproductive biotechnologies; in vitro embryo production

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gonzalez Grajales, L. A. (2015). Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

Chicago Manual of Style (16^th Edition):

Gonzalez Grajales, Leslie Antonio. “Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.” 2015. Doctoral Dissertation, University of Guelph. Accessed February 27, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638.

MLA Handbook (7^th Edition):

Gonzalez Grajales, Leslie Antonio. “Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer.” 2015. Web. 27 Feb 2021.

Vancouver:

Gonzalez Grajales LA. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. [Internet] [Doctoral dissertation]. University of Guelph; 2015. [cited 2021 Feb 27]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638.

Council of Science Editors:

Gonzalez Grajales LA. Development of bison embryos produced by parthenogenesis, interspecies somatic cell nuclear transfer, and ooplasm transfer. [Doctoral Dissertation]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8638

Universiteit Utrecht

7. Delft, T.V.A.F. van. Effect of climate on the success of an equine embryo transfer program.

Degree: 2006, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/34061

Subjects/Keywords: Diergeneeskunde; Climate, equine, embryo transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Delft, T. V. A. F. v. (2006). Effect of climate on the success of an equine embryo transfer program. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/34061

Chicago Manual of Style (16^th Edition):

Delft, T V A F van. “Effect of climate on the success of an equine embryo transfer program.” 2006. Doctoral Dissertation, Universiteit Utrecht. Accessed February 27, 2021. http://dspace.library.uu.nl:8080/handle/1874/34061.

MLA Handbook (7^th Edition):

Delft, T V A F van. “Effect of climate on the success of an equine embryo transfer program.” 2006. Web. 27 Feb 2021.

Vancouver:

Delft TVAFv. Effect of climate on the success of an equine embryo transfer program. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2006. [cited 2021 Feb 27]. Available from: http://dspace.library.uu.nl:8080/handle/1874/34061.

Council of Science Editors:

Delft TVAFv. Effect of climate on the success of an equine embryo transfer program. [Doctoral Dissertation]. Universiteit Utrecht; 2006. Available from: http://dspace.library.uu.nl:8080/handle/1874/34061

8. Braitner Matias Pereira. Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos.

Degree: 2008, Universidade Jose do Rosario Vellano

URL: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=68

►

O que se espera do tratamento superovulatorio de uma fêmea bovina utilizada como doadora em programas de transferência de embriões (TE) é que esta produza… (more)

▼

O que se espera do tratamento superovulatorio de uma fêmea bovina utilizada como doadora em programas de transferência de embriões (TE) é que esta produza estruturas em quantidade e qualidade. A nutrição e reprodução são dois aspectos que possuem estreitas interrelações, em qualquer sistema de produção. O Canter OF é um suplemento nutricional com vitaminas, minerais e aminoácidos, cujos constituintes poderiam melhorar os resultados de fêmeas bovinas submetidas à superovulação. O objetivo deste trabalho foi verificar os efeitos da administração do Canter OF sobre a resposta superovulatória e a produção embrionária de doadoras zebuínas submetidas aos programas de TE. Foram realizados 42 protocolos de superovulação, em 21 fêmeas bovinas doadoras da raça Guzerá, com peso corporal superior a 350 Kg, idade entre 20 e 60 meses e que estavam ciclando em intervalos regulares (213 dias). Os animais foram manejados em regime de semiconfinamento e receberam a mesma alimentação durante todo o período experimental, composta de volumoso e suplementação mineral ad libitum, visando manutenção do escore de condição corporal entre 3 e 4 (escala de 1 a 5). Todos os animais utilizados passaram pelo menos uma vez pelo grupo tratado (Canter OF) e pelo grupo controle num esquema de delineamento Cross-over. O produto foi administrado diariamente (20 ml) a cada animal, de forma individual por 30 dias antes da colheita dos embriões. Foram avaliadas as seguintes variáveis: influência dos tratamentos sobre a resposta ovariana ao protocolo superovulatório (número e diâmetro médio dos folículos no dia da inseminação), número de corpos lúteos presentes no dia da colheita de embriões, taxa de recuperação embrionária, total de estruturas produzidas, número de embriões viáveis ou degenerados e oócitos não fecundados e a taxa de gestação das receptoras. Os resultados mostraram que, quando são analisados todos os animais utilizados não se observaram diferenças (P>0,05) entre o total de corpos lúteos (10,202,04 e 9,79 1,73), total de embriões (7,642,06 e 6,641,71) e embriões viáveis (4,641,24 e 4,681,03) entre o grupo Controle e o Tratado com Canter OF, respectivamente. Quando se avaliaram apenas as vacas com pequena resposta superovulatória e produção embrionária ruim, os resultados do tratamento com Canter OF são diferentes. Para estes animais, algumas das variáveis estudadas foram superiores no grupo tratado com Canter OF. Neste grupo, o tratamento com Canter OF aumentou a resposta superovulatória, o total de embriões por coleta e o número de embriões viáveis (P<0,05). E os animais com histórico de resposta superovulatória boa não apresentaram nenhuma alteração estatisticamente significativa. Conclui-se que o produto, no protocolo utilizado foi eficiente em estimular maior número de ovulações, e produção de embriões, somente em doadoras com produção embrionária baixa.

A female bovine used as a donor in embryo transfer programs (ET) should produce structures with quality and quantity. The improvement of the efficiency of livestock production is a…

Advisors/Committee Members: Paulo de Figueiredo Vieira, Adauton de Vilela Rezende, Carlos Antônio de Carvalho Fernandes, Marilu Martins Gioso, João Henrique Moreira Viana.

Subjects/Keywords: bovino; nutrição; transferência de embrião; REPRODUCAO ANIMAL; bovine; nutrition; embryo transfer

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APA (6^th Edition):

Pereira, B. M. (2008). Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos. (Thesis). Universidade Jose do Rosario Vellano. Retrieved from http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pereira, Braitner Matias. “Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos.” 2008. Thesis, Universidade Jose do Rosario Vellano. Accessed February 27, 2021. http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pereira, Braitner Matias. “Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos.” 2008. Web. 27 Feb 2021.

Vancouver:

Pereira BM. Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos. [Internet] [Thesis]. Universidade Jose do Rosario Vellano; 2008. [cited 2021 Feb 27]. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=68.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pereira BM. Suplementação energética, vitamínica e mineral Canter OF. em doadoras de embriões bovinos. [Thesis]. Universidade Jose do Rosario Vellano; 2008. Available from: http://tede.unifenas.br/tde_busca/arquivo.php?codArquivo=68

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Correia, Sónia Cristina Gonçalves. Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates.

Degree: 2016, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/17021

► Embryo transfer (ET) is a biotechnology that allows to get more than one foal from a single mare during a breading season and also to… (more)

▼ Embryo transfer (ET) is a biotechnology that allows to get more than one foal from a single mare during a breading season and also to prevent the removal of mares from their competition careers. Nevertheless, to achieve a successful outcome, the association of many factors must be considered such as the management of donor and recipient mares, the stallions and the veterinarian´s experience. The aims of this study were to evaluate (1) if some of characteristics related to the donor mares such as the breed and the reproductive status, may influence the embryo recovery; (2) if climate and different donor mares location may have impact in breeding programs; (3) the effect of some reproductive techniques and embryo transfer methodologies such as the day of uterine flushing, the type of semen, the stallion and the moment of inseminations and transfer as potential effectors of success. To achieve the objectives pointed out, one hundred and fifty six uterine flushes and eighty eight embryo transfers were performed. Obtained results showed higher embryo recovery rates at day 8 (61.1%) and an overall pregnancy rate of 62.2%. An influence of the month of the year (P=0.04) and of the type of semen (fresh or frozen; P=0.03) on the number of positive embryo collections were identified. No other significant effects upon the remaining determinants under testing were observed, although stallions were responsible for inducing 11% of the variability within embryo recovery rates. In conclusion, the main factors that had a great influence in this embryo transfer program were the type of semen and the day and month of the uterine flushing procedure. The use of fresh semen for donor mares insemination and recovery at day 8 increased the number of embryos that were collected during this period, although lower results were obtained in July-August. These procedures should be consider and implement in mare embryo transfer centres. Advisors/Committee Members: Duarte, Sofia Alexandra Giestas Cancela, Pereira, Rosa Maria Lino Neto.

Subjects/Keywords: Artificial insemination; Embryo transfer; Equine; Month; Pregnancy; Semen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Correia, S. C. G. (2016). Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/17021

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Correia, Sónia Cristina Gonçalves. “Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates.” 2016. Thesis, RCAAP. Accessed February 27, 2021. https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/17021.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Correia, Sónia Cristina Gonçalves. “Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates.” 2016. Web. 27 Feb 2021.

Vancouver:

Correia SCG. Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates. [Internet] [Thesis]. RCAAP; 2016. [cited 2021 Feb 27]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/17021.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Correia SCG. Equine embryo transfer: the effect of semen processing and donor mare management on recovery rates. [Thesis]. RCAAP; 2016. Available from: https://www.rcaap.pt/detail.jsp?id=oai:comum.rcaap.pt:10400.26/17021

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Colorado State University

10. Kruse, Shantille. Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer.

Degree: MS(M.S.), Biomedical Sciences, 2012, Colorado State University

URL: http://hdl.handle.net/10217/65329

► The overall objective of my thesis research was to improve procedures for vitrifying bovine blastocysts so as to enable direct embryo transfer to the uterus.… (more)

▼ The overall objective of my thesis research was to improve procedures for vitrifying bovine blastocysts so as to enable direct embryo transfer to the uterus. Blastocysts were produced using standard in vitro procedures in Experiments 1, 2, and 3. Procedures were done at room temperature, 22 ± 2 °C. Unless otherwise mentioned, all media were made in SynGro®. In Experiment 1, base media contained either 1) normal concentrations of sodium (120 mM) and calcium (2 mM);(CON) or 2) 60 mM sodium + 60 mM choline chloride and 0.5 mM calcium (LOW). Blastocysts were exposed to 5 M ethylene glycol (V1) for 3 min and moved to 6.5 M ethylene glycol + 0.5 M galactose + 18% Ficoll (V2). Straws (0.25 mL) were loaded with a column of 120 μl 1 M galactose followed by an air bubble, then V2 containing embryos followed by an air bubble, and 60 μl 1 M galactose followed by sealing with a plastic plug. After 35 s, embryos were vitrified by either 1) standard cooling in liquid nitrogen cooled air (AIR) for 1 min or 2) cooling via contact of straw walls with columns drilled into an aluminum block immersed in liquid nitrogen (BLK) for 2 min and then directly plunged into liquid nitrogen. These combinations resulted in 4 treatments (AIR x CON; n = 61, AIR x LOW; n = 58, BLK x CON; n = 73, BLK x LOW; n = 54). BLK Embryos were warmed by holding straws in air for 10 s, placing them in a water bath at 37 °C for 20 s, mixing embryos with galactose diluent in the straw for 2 min and expelling. Embryos were recovered, rinsed through holding medium, and cultured in chemically defined medium (similar to synthetic oviduct fluid (SOF)) for 24 h before being evaluated for survival. Post warming survival did not differ (P > 0.10) between treatments (AIR x CON = 42.0%; AIR x LOW = 26.8%; BLK x CON = 21.8%, BLK x LOW = 24.5%). Despite lack of statistical significance, we recommend use of LOW base media because both sodium and calcium levels are reduced. Use of this media should therefore have less chance of sodium and calcium toxicity, and could deter apoptosis. The BLK vitrification method is both easier to use and more consistent. In Experiment 2, we sought to identify the most efficacious cryopreservation method for in vitro-produced bovine blastocysts that would enable direct embryo transfer from 0.25 mL straws used as containers for cryopreservation. Although not a method for direct transfer, Cryotops were chosen to serve as positive controls (CON), as they are the industry standard for vitrification of human embryos. Embryos were cryopreserved by vitrification with a Cryotop (CON; n = 118), using an aluminum block (BLK; n = 128), or by slow freezing (SLF; n = 131). Vitrification procedures were as described above for BLK with the exception that CON embryos were placed in < 1 μl V2 onto Cryotops, and after 35 s, vitrified by plunging directly into liquid nitrogen. Embryos cryopreserved via SLF were exposed to 1.36 M glycerol in modified Dulbecco's PBS + 0.4% BSA (PBS) for 10 min, loaded into 0.25 mL straws, and placed… Advisors/Committee Members: Seidel, George, Jr. E. (advisor), Ahola, Jason K. (committee member), Bruemmer, Jason E. (committee member).

Subjects/Keywords: bovine; direct transfer; embryo; in vitro; in vivo; vitrification

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kruse, S. (2012). Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/65329

Chicago Manual of Style (16^th Edition):

Kruse, Shantille. “Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer.” 2012. Masters Thesis, Colorado State University. Accessed February 27, 2021. http://hdl.handle.net/10217/65329.

MLA Handbook (7^th Edition):

Kruse, Shantille. “Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer.” 2012. Web. 27 Feb 2021.

Vancouver:

Kruse S. Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer. [Internet] [Masters thesis]. Colorado State University; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10217/65329.

Council of Science Editors:

Kruse S. Vitrification of in vitro- and in vivo-produced bovine embryos for direct transfer. [Masters Thesis]. Colorado State University; 2012. Available from: http://hdl.handle.net/10217/65329

Universitat Politècnica de València

11. García Domínguez, Ximo. Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus .

Degree: 2021, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/149562

► [ES] Las tecnologías de reproducción asistida suponen un cambio drástico en el entorno natural del embrión, al no conseguir imitar las condiciones maternales óptimas, por… (more)

▼ [ES] Las tecnologías de reproducción asistida suponen un cambio drástico en el entorno natural del embrión, al no conseguir imitar las condiciones maternales óptimas, por lo que su aplicación implica consecuencias para el desarrollo del organismo. El objetivo general de esta tesis fue estudiar los efectos a largo plazo y transgeneracionales del estrés provocado durante un procedimiento de transferencia de embriones vitrificados, utilizando el conejo como modelo animal. En el Capítulo I, demostramos que la transferencia de mórulas tempranas o compactas resulta en tasas de supervivencia al parto > 70% en fresco y > 55% tras la vitrificación. La facilidad con la que se pueden realizar estos procedimientos, el elevado número de descendientes que podemos obtener y el corto ciclo de vida del conejo, fomentaron y facilitaron los siguientes estudios. En el Capítulo II, se compararon las diferencias en el desarrollo a corto y largo plazo entre los animales nacidos de embriones transferidos en fresco (FT) o tras su vitrificación (VT), utilizando una población concebida de forma natural (NC) como referencia. Tanto las tasas de supervivencia prenatal, como el rendimiento del crecimiento postnatal, se redujeron significativamente a medida que aumentó la manipulación embrionaria (NC<FT<VT). Además, comparamos el efecto de dos dispositivos de vitrificación, observando que, aunque el cryotop ejerció un efecto positivo sobre la supervivencia fetal, implicó mayores desviaciones fenotípicas (rendimiento de crecimiento y lactancia) postnatalmente que el dispositivo de la ministraw. Sin embargo, todas las progenies fueron sanas y fértiles. Por lo tanto, estos resultados demostraron la gran plasticidad del embrión de mamífero. El objetivo del Capítulo III fue evaluar los efectos del procedimiento de transferencia de embriones vitrificados (VET) en el desarrollo, comparando animales VT y NC. Así, detectamos que los animales VT presentaron alteraciones del peso al nacer y del patrón de crecimiento, viéndose los machos más afectados que las hembras. En la edad adulta, los machos VT presentaron un menor peso corporal, del hígado y del corazón. Un análisis proteómico hepático mostró cambios en relación con la fosforilación oxidativa y el metabolismo de los lípidos y el zinc. Mediante un análisis de sangre, se comprobó que el estado de salud entre los animales VT y NC fue comparable. En el Capítulo IV, se constituyó un modelo de tres generaciones (F1, F2 y F3) para evaluar los efectos transgeneracionales del VET. Los resultados mostraron que los efectos directos (F1) del VET fueron intergeneracionales (F2) y transgeneracionales (F3), ya que las progenies VT mostraron alteraciones en el crecimiento, peso corporal adulto y peso hepático. Un estudio molecular (transcriptómico y metabolómico) del tejido hepático reveló alteraciones en el metabolismo del zinc y los ácidos grasos insaturados a través de las generaciones, correlacionado con el fenotipo VT. No obstante, la fertilidad fue similar entre los machos VT y NC en cada… Advisors/Committee Members: Marco Jiménez, Francisco (advisor), Vicente Antón, José Salvador (advisor).

Subjects/Keywords: Embryo; Cryopreservation; Vitrification; Transfer; Plasticity; Development; Transgenerational; Rabbit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

García Domínguez, X. (2021). Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/149562

Chicago Manual of Style (16^th Edition):

García Domínguez, Ximo. “Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus .” 2021. Doctoral Dissertation, Universitat Politècnica de València. Accessed February 27, 2021. http://hdl.handle.net/10251/149562.

MLA Handbook (7^th Edition):

García Domínguez, Ximo. “Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus .” 2021. Web. 27 Feb 2021.

Vancouver:

García Domínguez X. Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2021. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10251/149562.

Council of Science Editors:

García Domínguez X. Developmental plasticity and transgenerational reprogramming following vitrified embryo transfer in Oryctolagus cuniculus . [Doctoral Dissertation]. Universitat Politècnica de València; 2021. Available from: http://hdl.handle.net/10251/149562

University of Adelaide

12. Kaur, Harleen. A novel intervention strategy to prevent intrauterine growth restriction.

Degree: 2020, University of Adelaide

URL: http://hdl.handle.net/2440/128672

► Intrauterine growth restriction (IUGR) increases risks of perinatal death >5-fold, and has lifelong adverse effects on health. There is currently no treatment to prevent or… (more)

▼ Intrauterine growth restriction (IUGR) increases risks of perinatal death >5-fold, and has lifelong adverse effects on health. There is currently no treatment to prevent or cure IUGR, although animal studies have shown promising effects of maternal growth hormone (GH) treatment on fetal and placental growth. The overall aim of this project was to test a novel approach to stimulate the mother’s own production of GH, to promote placental function and fetal growth in normal and IUGR pregnancies, and hence develop a therapy to prevent IUGR which is acceptable to patients. My first aim was to investigate whether the abundance or activation of the GH secratagogue, ghrelin, increased during pregnancy or in response to dietary supplementation with octanoic acid, which is required for activation (acylation) of ghrelin. Maternal circulating total and acyl-ghrelin and GH profiles were measured in late pregnant mice and in aged-matched non-pregnant females, fed a diet supplemented with octanoic or palmitic (control) acid. However, dietary supplementation did not increase the activation of ghrelin in female mice, whilst the pregnancy-associated increase in maternal GH secretion occurred without elevated circulating acyl-ghrelin concentrations. The second aim was to develop a novel method to measure placental function, a major determinant of fetal growth, using non-radioactive tracers in mice. A dye containing fluorescently-labelled glucose was injected into pregnant dams near term, and its maternal, placental and fetal uptake were characterised. Although the results indicated that this tracer was not specific for placental glucose uptake, its uptake provided a good indication of overall placental function and fetal nutrient uptake. Lastly, in order to test potential interventions, a murine model of IUGR in multiple pregnancies was established. Mouse pregnancies were generated by transferring variable numbers of embryos into pseudo-pregnant mice, generating litters with varying degrees of maternal constraint near term. Fetuses from the largest litters were ~25% lighter as well as thinner and with evidence of brain-sparing, compared to fetuses from smaller litters. Interestingly, the relationship between viable litter size and fetal weight differed between sexes, such that fetal weights of males, but not females, correlated negatively with litter size. However, this sex difference was not explained by morphological and functional changes in the placenta. The results of this experiment suggested that male fetuses grew as fast as permitted by nutrient supply, whereas the female maintained placental reserve capacity. This strategy, likely reflecting sex-specific gene expression, would be expected to place the male fetus at greater risk of death in the face of any additional intrauterine stressors. In conclusion, the dietary supplementation trialled in this project did not promote fetal growth in normal pregnancies or stimulate responses in the mother that would promote fetal growth in growth restricted pregnancies. However, this… Advisors/Committee Members: Gatford, Kathy (advisor), Muhlhausler, Beverly (advisor), Roberts, Claire (advisor), Adelaide Medical School (school).

Subjects/Keywords: IUGR; growth hormone; pregnancy; diet; placenta; mouse; embryo transfer; fluorophore

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kaur, H. (2020). A novel intervention strategy to prevent intrauterine growth restriction. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/128672

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kaur, Harleen. “A novel intervention strategy to prevent intrauterine growth restriction.” 2020. Thesis, University of Adelaide. Accessed February 27, 2021. http://hdl.handle.net/2440/128672.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kaur, Harleen. “A novel intervention strategy to prevent intrauterine growth restriction.” 2020. Web. 27 Feb 2021.

Vancouver:

Kaur H. A novel intervention strategy to prevent intrauterine growth restriction. [Internet] [Thesis]. University of Adelaide; 2020. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2440/128672.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaur H. A novel intervention strategy to prevent intrauterine growth restriction. [Thesis]. University of Adelaide; 2020. Available from: http://hdl.handle.net/2440/128672

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

13. Wulster, Meghan Carole. Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo.

Degree: MS, Animal and Poultry Sciences, 1997, Virginia Tech

URL: http://hdl.handle.net/10919/36942

► Experiments were initiated to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to dilate the cervix and improve transcervical embryo transfer (ET)… (more)

▼ Experiments were initiated to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to dilate the cervix and improve transcervical embryo transfer (ET) procedures for sheep. However, there was concern that the E2-OT treatment may alter luteal function and that embryo quality would decrease as the superovulatory response to FSH increased. In Exp. 1, 32 ewes were assigned to a 2 x 2 factorial array of treatments. On d 7, ewes received an i.v. injection of either 100 micrograms of E2 in 5 mL of 1:1 ethanol:saline or 5 mL of 1:1 ethanol:saline; 12 h later, ewes received i.v. injection of either 400 USP units of OT or saline. Jugular blood was collected on d 7, 8, 9, 10, 12, 14, 16, and 18. Progesterone concentrations were unaffected by the treatments. Experiment 2 was conducted to determine the dose of pFSH needed to induce approximately six corpora lutea (CL). Ten-day Norgestomet implants inserted between d 8-12 of the estrous cycle were used to synchronize estrus in Hampshire and Hampshire x Dorset ewes (n = 23). Ewes received a total of either 0, 18, 27, or 36 mg of pFSH, which was injected i.m. at -24, -12, 0, 12, 24, and 36 h relative to implant removal. The dose at each respective time was 19.4, 19.4, 16.7, 16.7, 13.9, and 13.9% of the total. Ewes received 400 IU of PMSG i.m. at -24 h. The CL were counted laparoscopically on d 6 (d 0 = estrus). Number of CL increased linearly (P < .01) with dose of pFSH; there were 1.8, 3.6, 6.3, and 11.2 CL/ewe, respectively. Experiment 3 was conducted to determine the effect of the E2-OT treatment, mode of transfer or the interaction of E2-OT treatment x mode of transfer on embryo survival and development. Experiment 3 was conducted over two breeding seasons and across two trials. In the first trial ewes were assigned to one of three randomized treatments. Procedural limitations that were later overcome prevented a true 2 x 2 factorial design; therefore, transcervical transfer without hormonal treatment was excluded in the first trial. In the second trial, ewes were assigned to a 2 x 2 factorial array of treatments. On d 6 of pregnancy, embryos rating a fair or better were transferred into recipients either transcervically or laparoscopically. Recipients were administered either an E2 (d 6) - OT (d 7) treatment or an ethanol:saline-saline treatment following the same protocol as in Exp. 1. Embryos were recovered on d 12 in Trial 1 and d 14 in Trial 2. Embryos were evaluated morphologically for development and ranked on a scale of one to four; one represented no development and four represented development to the morphological stages associated with the day of collection. The treatments did not affect the percentage of embryos recovered after transfer or the percentage of embryos that showed some developed. However, there was an effect of mode of transfer on mean rank of embryo development; embryos transferred laporscopically developed further than embryos transferred transcervically (P < .01). This may have been an artifact of a technician… Advisors/Committee Members: Lewis, Gregory S. (committeechair), Saacke, Richard G. (committee member), Knight, James W. (committee member).

Subjects/Keywords: Embryo Transfer; Oxytocin; Sheep

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wulster, M. C. (1997). Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/36942

Chicago Manual of Style (16^th Edition):

Wulster, Meghan Carole. “Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo.” 1997. Masters Thesis, Virginia Tech. Accessed February 27, 2021. http://hdl.handle.net/10919/36942.

MLA Handbook (7^th Edition):

Wulster, Meghan Carole. “Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo.” 1997. Web. 27 Feb 2021.

Vancouver:

Wulster MC. Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo. [Internet] [Masters thesis]. Virginia Tech; 1997. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10919/36942.

Council of Science Editors:

Wulster MC. Estradiol-17beta-Oxytocin Induced Cervical Dilation in Sheep: Application to Transcervical Embryo. [Masters Thesis]. Virginia Tech; 1997. Available from: http://hdl.handle.net/10919/36942

University of Sydney

14. Li, Zhuoyang. Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology .

Degree: 2019, University of Sydney

URL: http://hdl.handle.net/2123/20330

► Background and aims: Assisted reproductive technology has provided great hope for millions of infertile couples. In recent years, frozen-thawed embryo transfer has no longer been… (more)

Subjects/Keywords: assisted reproductive technology; freeze-only; cryopreservation; embryo transfer; pregnancy outcomes

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Z. (2019). Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/20330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Zhuoyang. “Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology .” 2019. Thesis, University of Sydney. Accessed February 27, 2021. http://hdl.handle.net/2123/20330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Zhuoyang. “Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology .” 2019. Web. 27 Feb 2021.

Vancouver:

Li Z. Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology . [Internet] [Thesis]. University of Sydney; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2123/20330.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li Z. Fertility and pregnancy outcomes following fresh versus frozen-thawed embryo transfer in assisted reproductive technology . [Thesis]. University of Sydney; 2019. Available from: http://hdl.handle.net/2123/20330

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

15. Ginström Ernstad, Erica. Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques.

Degree: 2020, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/63272

► Background: Singletons born following assisted reproductive technology (ART) have adverse neonatal outcome compared to singletons born following spontaneous conception (SC). Moreover, the women undergoing ART… (more)

▼ Background: Singletons born following assisted reproductive technology (ART) have adverse neonatal outcome compared to singletons born following spontaneous conception (SC). Moreover, the women undergoing ART are at an increased risk of hypertensive disorders in pregnancy (HDP) and placental complications. Aim: To study the neonatal and maternal outcomes following the introduction of advanced techniques in ART. Material and methods: All papers were population-based register studies in Sweden with cross linkage of the national ART registers and national health data registers. In paper III also Danish register data were included. Paper I Singletons born after blastocyst transfer (n=4819), singletons born after cleavage stage transfer (n=25,747) and singletons born after SC (n=1,196,394) were included. The main outcome was birth defects. Moreover, other neonatal and maternal outcomes were assessed. Paper II Neonatal and maternal outcomes in different cycle regimens in frozen embryo transfer (FET) (n=6297 in natural cycles, n=1983 in stimulated cycles, n=1446 in programmed cycles) were studied. FET was also compared to fresh embryo transfer and to SC. The primary outcomes were preterm birth (PTB, <37 weeks), low birth weight (LBW, <2500 grams), HDP and postpartum hemorrhage (PPH, >1000 mL). Paper III Singleton pregnancies following transfer of vitrified blastocysts (n=3650) were compared to singleton pregnancies following slow-frozen cleavage stage transfer (n=8123) and fresh blastocyst transfer (n=4469). Main outcomes were PTB, LBW, macrosomia, HDP and PPH. Paper IV Singletons born following preimplantation genetic testing (PGT) (n=267) were compared to singletons born following in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) (n=55,355) and to SC (n=26,535). Main outcomes were PTB and LBW. Moreover, maternal outcomes and early childhood outcome were assessed. Results: Paper I No difference in the rate of birth defects were observed between the groups. However, there was an increased risk of placenta previa and placental abruption following blastocyst transfer compared to transfer of cleavage stage embryos and SC. Paper II Programmed cycles were associated with a higher risk of HDP (adjusted odds ratio [AOR] 1.6-1.8), PPH (AOR 2.6-2.9), post term birth (AOR 1.6-2.0) and macrosomia (≥4500 grams) (AOR 1.4-1.6) compared to other cycle regimens. The rates of PTB and LBW were similar independently of cycle regimen. Paper III Transfer of vitrified blastocysts was associated with a higher risk of PTB (AOR 1.3). No other differences were found. Paper IV For PGT singletons no differences in PTB and LBW were observed in comparison to other IVF/ICSI singletons yet higher rates compared to SC. The early childhood outcomes were reassuring but should be interpreted cautiously due to few cases and short follow-up time. Conclusion: Blastocyst transfer is associated with a higher risk of placenta previa and placental abruption compared to cleavage stage transfer. Programmed cycles were associated with higher risks of…

Subjects/Keywords: blastocyst transfer; frozen embryo transfer; vitrification; preimplantation genetic testing; neonatal outcome; maternal outcome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ginström Ernstad, E. (2020). Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/63272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ginström Ernstad, Erica. “Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques.” 2020. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed February 27, 2021. http://hdl.handle.net/2077/63272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ginström Ernstad, Erica. “Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques.” 2020. Web. 27 Feb 2021.

Vancouver:

Ginström Ernstad E. Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2020. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/2077/63272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ginström Ernstad E. Safety and quality aspects of IVF - neonatal and maternal outcomes following advanced techniques. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2020. Available from: http://hdl.handle.net/2077/63272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Technical University of Lisbon

16. Carvalho, Ana Lúcia. Fatores que influenciam o sucesso de um programa de transferência de embriões equinos.

Degree: 2012, Technical University of Lisbon

URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4849

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Dissertação de Mestrado Integrado em Medicina Veterinária

O sucesso de um programa comercial de transferência de embriões depende principalmente da interação dos fatores que afetam… (more)

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Dissertação de Mestrado Integrado em Medicina Veterinária

O sucesso de um programa comercial de transferência de embriões depende principalmente da interação dos fatores que afetam a recolha embrionária, a gestação e a morte embrionária após a transferência. O objetivo deste trabalho consistiu na avaliação de alguns destes fatores, de forma a minimizar aqueles que possam ter uma influência negativa e permitindo também informar e aconselhar os proprietários dos animais de uma forma mais clara e correta. O presente estudo foi realizado na Central Equina de Reprodução, no estado de São Paulo, Brasil. Foram analisados os dados relativos a 2086 recolhas e 1090 transferências, efetuadas ao longo de duas épocas reprodutivas consecutivas. Neste trabalho, foi avaliada a influência na taxa de recolha embrionária, da época reprodutiva, do dia da recolha, do tipo de sémen (refrigerado ou congelado) utilizado na inseminação artificial e da raça da dadora. Relativamente às taxas de gestação e de morte embrionária, foi avaliada a influência da época reprodutiva, da idade do embrião, da ciclicidade (recetoras cíclicas e acíclicas) e do dia pós-ovulação em que a recetora se encontrava no dia da transferência. As variáveis foram analisadas pelo teste de Fisher ou Qui-quadrado em tabelas de contingência 2x2, sendo as diferenças consideradas significativas se p <0,05. O tipo de sémen teve uma influência significativa na taxa de recolha embrionária. Foi possível concluir que em éguas inseminadas com sémen congelado a taxa de recolha embrionária é menor (p <0,05), sendo por isso preferível utilizar sémen fresco ou refrigerado. A época reprodutiva afetou significativamente a taxa de gestação, tendo esta sido superior no ano de 2011-2012 (p <0,05). No entanto, em ambas as épocas reprodutivas, as taxas de gestação foram consideradas satisfatórias, sendo de 76,0% em 2010-2011 e de 81,8% em 2011-2012. Tanto a idade do embrião como a ciclicidade e o dia pós-ovulação da recetora não influenciaram as taxas de gestação nem as de morte embrionária. Estes resultados permitem concluir que é possível transferir embriões entre os 6 e os 10 dias de idade, utilizar éguas recetoras entre os 3 e os 9 dias pós-ovulação bem como éguas em anestro suplementadas com progesterona de longa ação, sem que isso afete o sucesso do programa de transferências.

The success of a commercial embryo transfer program depends mainly on the interaction of factors that affect embryo recovery, pregnancy and embryonic loss after the embryo transfer. The objective of this study was to evaluate these factors, allowing us to minimize the ones that can have a negative influence, and simultaneously to correctly inform and give advice to animal owners. Data of 2086 collections and 1090 transfers made over two consecutive breeding seasons in the Central Equina de Reprodução in the state of São Paulo, Brazil were analysed. In this study, it was assessed the influence of reproductive season, collection day, type of semen used in artificial insemination (cooled or frozen), and donor…

Advisors/Committee Members: Ávila Jr., Orpheu de Souza, Dias, Graça Maria Leitão Ferreira.

Subjects/Keywords: Transferência de embriões; recolha embrionária; taxa de gestação; taxa de morte embrionária; Embryo transfer; embryo recovery; pregnancy rates; embryonic loss rate

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carvalho, A. L. (2012). Fatores que influenciam o sucesso de um programa de transferência de embriões equinos. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carvalho, Ana Lúcia. “Fatores que influenciam o sucesso de um programa de transferência de embriões equinos.” 2012. Thesis, Technical University of Lisbon. Accessed February 27, 2021. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carvalho, Ana Lúcia. “Fatores que influenciam o sucesso de um programa de transferência de embriões equinos.” 2012. Web. 27 Feb 2021.

Vancouver:

Carvalho AL. Fatores que influenciam o sucesso de um programa de transferência de embriões equinos. [Internet] [Thesis]. Technical University of Lisbon; 2012. [cited 2021 Feb 27]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4849.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carvalho AL. Fatores que influenciam o sucesso de um programa de transferência de embriões equinos. [Thesis]. Technical University of Lisbon; 2012. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4849

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

17. Lima, Wagner Marques de. Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus).

Degree: 2007, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/10414

►

A influência do momento da ablação do folículo dominante antes do início do tratamento superovulatório, e seu efeito sobre a dinâmica folicular ovariana e a… (more)

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A influência do momento da ablação do folículo dominante antes do início do tratamento superovulatório, e seu efeito sobre a dinâmica folicular ovariana e a taxa de produção de embriões, ainda permanece indeterminada em bovinos. O presente estudo foi desenvolvido com objetivo de avaliar a resposta superovulatória de fêmeas da raça Limousin, determinando os efeitos da influência do dia de início da superovulação (SOV) de animais no diestro; da presença ou ausência de folículos palpáveis nos ovários no momento de início do tratamento (status ovariano); e do tempo, em horas, entre a ablação folicular e o início da SOV. Foram realizadas 244 lavagens uterinas para coleta de embriões em novilhas (n=98) e vacas (n=146), divididas em cinco grupos de acordo com o status ovariano no momento de início da SOV, durante o intervalo de 8 a 12 dias após o estro: grupo sem folículo perceptível (n=106); grupo com folículo mantido intacto (n=62) e grupos com folículo puncionado 48 h (n=10), 24 h (n=35) e 0 h (n=31) antes do início da SOV. Nos grupos em que foi realizada a ablação, utilizou-se uma cânula de metal contendo uma agulha para transposição da parede vaginal e ovariana até a aspiração do conteúdo folicular sob controle tátil por via retal. Os resultados obtidos determinaram que a presença de folículos palpáveis no momento de início da SOV reduz significativamente a resposta superovulatória. Os dados de ablação folicular realizada imediatamente ou até 24 h antes do início do tratamento de SOV aumentaram significativamente o número total de embriões viáveis. Entretanto, a resposta do tratamento superovulatório não foi afetada pelo dia do início da aplicação de gonadotrofina no período de 8 a 12 dias após o estro e nem pela categoria animal (novilha ou vaca). Em conclusão, a ablação de folículos perceptíveis por palpação retal entre os dias 8 a 12 do ciclo estral, quando realizada até 24 h antes do início do tratamento de SOV, aumentou o número total de embriões transferíveis por coleta em gado de corte (Bos taurus taurus).

The influence of the timing for the ablation of dominant follicle prior to superovulatory treatment, and its effect on ovarian follicular growth and embryo yield, still remain elusive in cattle. The present study was designed to evaluate the superovulatory response of Limousin cattle, aiming to determine the effect of the day at mid-diestrus for the onset of superovulation (SOV); presence or absence of large ovarian follicles (ovary status); and the time of follicular ablation, in hours, prior to the SOV. A total of 244 uterine flushings for embryo collections were made using two female categories (heifers or cows) at 8 to 12 days after the estrous. Based on ovary status, the females were allocated into 5 groups: group without palpable follicle(s) (n=106); group with intact follicle(s) (n=62), and groups with follicle ablation at 48 h (n=10), 24 h (n=35) and 0 h (n=31) before the beginning of SOV. Follicles were aspirated transvaginally using a specially designed custom-built steel cannula for follicular…

Advisors/Committee Members: Gregory, Ricardo Macedo.

Subjects/Keywords: Transferência embrionária; Embryo transfer; Superovulacao : Bovinos; Follicular ablation; Indução da ovulação; Superovulation; Bovine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lima, W. M. d. (2007). Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus). (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/10414

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lima, Wagner Marques de. “Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus).” 2007. Thesis, Universidade do Rio Grande do Sul. Accessed February 27, 2021. http://hdl.handle.net/10183/10414.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lima, Wagner Marques de. “Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus).” 2007. Web. 27 Feb 2021.

Vancouver:

Lima WMd. Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus). [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2007. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10183/10414.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lima WMd. Resposta superovulatória após ablação folicular usando um dispositivo simplificado em bovinos (Bos taurus taurus). [Thesis]. Universidade do Rio Grande do Sul; 2007. Available from: http://hdl.handle.net/10183/10414

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Monteiro Junior, Pedro Leopoldo Jerônimo. Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows.

Degree: PhD, Ciência Animal e Pastagens, 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-28042015-093330/ ;

►

In recent years, in dairy cattle, while it was observed a gradual increase in productivity, a decrease occurred in the reproductive efficiency. Several factors, such… (more)

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In recent years, in dairy cattle, while it was observed a gradual increase in productivity, a decrease occurred in the reproductive efficiency. Several factors, such as increased incidence of diseases, higher susceptibility to heat stress and increase of dry matter intake, have been awarded as possible causes for the decrease in fertility. Increased dry matter intake is associated with increased liver blood flow, which is associated with an increase in liver metabolism of steroid hormones. Given the high metabolism of steroid hormones in high producing dairy cows, six studies were carried out, which in this thesis are divided in three chapters, involving hormone supplementation in lactating dairy cows. The first study aimed to increase the synchronization rate of dairy cows submitted to a fixed time artificial insemination (FTAI) estradiol (E2)/progesterone (P4)-based protocol. For this purpose, two experiments were performed, the first (n = 44 cows) compared a 2.0 vs 3.0 mg of estradiol benzoate (EB) associated to a P4 implant at the beginning of the protocol. The second experiment (n = 82 cows) performed presynchronization with GnRH prior to the onset of a FTAI protocol to produced different follicular development stages at the time of E2/P4: emergence vs. dominance. Daily ultrasound and hormone evaluations were performed. Other four experiments are described in the second (n = 1070 cows) and third (n = 1498 cows) chapter, which have been developed to evaluate the effect of P4 supplementation after ovulation in lactating dairy cows. In general, these studies evaluated the effect of supplementation on the corpus luteum (CL) development and function, mRNA abundance for interferon stimulated genes (ISG), on fertility of cows subjected to AI after estrus detection or FTAI protocol, or to embryo transfer. Increasing the EB dose from 2.0 for 3.0 mg did not improve emergence wave synchronization. In fact, it induced luteolysis in a larger number of cows. Altering the stage of the estrous cycle of the cows at the beginning of the E2/P4-based FTAI protocol did not improve synchronization of wave emergence. Post ovulation P4 supplementation did not affect CL development and function, and did not increase the mRNA abundance for ISG. Cows subjected to AI after estrus detection or after an E2/P4-based FTAI protocol did not have increased fertility. However when P4-supplemented cows were subjected to a GnRH-based FTAI protocol there was an improvement in the fertility of about 8%. Thus, we can concluded that regardless of the EB dose or stage of the estrous cycle at beginning of the E2/P4-based FTAI protocol, still there are cows that fail to have a synchronized emergence of a new wave and/or to ovulate at the end protocol. Additionally, depending on the protocol used, P4 supplementation may increase the fertility of dairy cows, but compromises the fertility when embryos are transferred.

Nos últimos anos, em rebanhos leiteiros, foi observado um aumento acentuado da produtividade acompanhado de uma diminuição da eficiência…

Advisors/Committee Members: Sartori Filho, Roberto.

Subjects/Keywords: Artificial insemination; Embryo transfer; Estradiol; Estradiol; Inseminação artificial; Progesterona; Progesterone; Sincronização; Synchronization; Transferência de embrião

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Monteiro Junior, P. L. J. (2015). Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/11/11139/tde-28042015-093330/ ;

Chicago Manual of Style (16^th Edition):

Monteiro Junior, Pedro Leopoldo Jerônimo. “Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows.” 2015. Doctoral Dissertation, University of São Paulo. Accessed February 27, 2021. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-28042015-093330/ ;.

MLA Handbook (7^th Edition):

Monteiro Junior, Pedro Leopoldo Jerônimo. “Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows.” 2015. Web. 27 Feb 2021.

Vancouver:

Monteiro Junior PLJ. Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Feb 27]. Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-28042015-093330/ ;.

Council of Science Editors:

Monteiro Junior PLJ. Exogenous hormonal manipulation to increase reproductive efficiency in dairy cows. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/11/11139/tde-28042015-093330/ ;

19. Sigalos, Georgios. Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF).

Degree: 2019, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/45569

►

Over the last few years, many studies have focused on embryo selection methods, whereas little attention has been given to the standardization of the IVF… (more)

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Over the last few years, many studies have focused on embryo selection methods, whereas little attention has been given to the standardization of the IVF and embryo transfer procedure. The volume of the culture medium transferred into the uterine cavity is a variable that has been suggested to affect the IVF outcome. The aim of this prospective randomized control trial was to evaluate if the use of two different volumes (20-25 μl VS 40-45 μl) of media used for embryo transfer affects the clinical outcomes in fresh in vitro fertilization cycles (IVF).In 2016 a review of the literature was conducted to review the variables affecting the embryo transfer and to compare the existing embryo transfer techniques. In parallel, 236 patients were randomized in two groups i.e. ‘low volume group’ (n=118) and ‘high volume group’ (n=118) based on the volume used for the embryo transfer. The clinical pregnancy, implantation and ongoing pregnancy rates were compared between the two groups.No statistically significant differences were observed in clinical pregnancy (46.8 vs 54.3 %, p=0.27), implantation (23.7 vs 27.8%, p=0.30) and ongoing pregnancy (33.3 vs 40.0%, p=0.31) rates between low and high volume group, respectively. Consequently, higher volume of culture medium to load the embryo into the catheter during embryo transfer does not influence the clinical outcome in fresh IVF cycles.

Τα τελευταία χρόνια πολλές μελέτες έχουν επικεντρωθεί κυρίως στις μεθόδους επιλογής των καταλληλότερων εμβρύων για εμβρυομεταφορά ωστόσο λίγη προσοχή έχει δοθεί στην τυποποίηση των τεχνικών της εξωσωματικής γονιμοποίησης και της εμβρυομεταφοράς. Μία από τις παραμέτρους που έχει προταθεί ότι επηρεάζει το αποτέλεσμα της εξωσωματικής γονιμοποίησης είναι ο όγκος του καλλιεργητικού υλικού που μεταφέρεται στην ενδομητρική κοιλότητα κατά την εμβρυομεταφορά. Σκοπός της παρούσας προοπτικής τυχαιοποιημένης μελέτης ήταν να αξιολογηθεί εάν η χρήση δύο διαφορετικών όγκων καλλιεργητικού υλικού (20-25 μl VS 40-45 μl) κατά την εμβρυομεταφορά επηρεάζει το αποτέλεσμα φρέσκων κύκλων εξωσωματικής γονιμοποίησης.Το 2016 πραγματοποιήθηκε πλήρης βιβλιογραφική ανασκόπηση για την καταγραφή των παραγόντων που επηρεάζουν την εμβρυομεταφορά και την σύγκριση των υπαρχόντων τεχνικών εμβρυομεταφοράς. Παράλληλα, 236 ασθενείς κατανεμήθηκαν τυχαία σε δύο ομάδες (μικρού όγκου,n=118 και μεγάλου όγκου, n=118 ) ανάλογα με τον όγκο καλλιεργητικού υλικού που μεταφέρθηκε στην εμβρυομεταφορά. Το ποσοστό κλινικών κυήσεων, το ποσοστό εμφύτευσης και το ποσοστό συνεχιζόμενων κυήσεων συγκρίθηκε ανάμεσα στις δύο ομάδες.Δεν παρατηρήθηκε καμία στατιστικά σημαντική διαφορά στο ποσοστό κλινικών κυήσεων (46.8 vs 54.3 %, p=0.27), ποσοστό εμφύτευσης (23.7 vs 27.8%, p=0.30) και συνεχιζόμενων κυήσεων (33.3 vs 40.0%, p=0.31) μεταξύ μικρού και μεγάλου όγκου αντίστοιχα. Συνεπώς, μεγαλύτερος όγκος καλλιεργητικού υλικού για την ‘φόρτωση’ των εμβρύων στον καθετήρα δεν επηρεάζει τα κλινικά αποτελέσματα σε φρέσκους κύκλους εξωσωματικής γονιμοποίησης.

Subjects/Keywords: Τεχνικές εμβρυομεταφοράς; Εξωσωματική γονιμοποίηση; Εμβρυολογικό εργαστήριο; Embryo transfer techniques; In vitro fertilization; Embryology Laboratory

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sigalos, G. (2019). Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF). (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/45569

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sigalos, Georgios. “Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF).” 2019. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed February 27, 2021. http://hdl.handle.net/10442/hedi/45569.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sigalos, Georgios. “Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF).” 2019. Web. 27 Feb 2021.

Vancouver:

Sigalos G. Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF). [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10442/hedi/45569.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sigalos G. Επίδραση διαφορετικών τεχνικών φόρτωσης του καθετήρα εμβρυομεταφοράς στα αποτελέσματα κύκλων εξωσωματικής γονιμοποίησης (IVF). [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2019. Available from: http://hdl.handle.net/10442/hedi/45569

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Paula Gomes Rodrigues. Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares.

Degree: 2009, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=2674

►

Body condition scoring (BCS) was compared to ultrasound subcutaneous fat thickness (SFT) determination and their relation to the reproductive efficiency of Mangalarga Marchador embryo donor… (more)

▼

Body condition scoring (BCS) was compared to ultrasound subcutaneous fat thickness (SFT) determination and their relation to the reproductive efficiency of Mangalarga Marchador embryo donor mares. Fifty six barren mares were studied throughout the 2008/2009 breeding season (August through April). Body condition score, evaluated monthly, was obtained form the average of two evaluators on a 1-9 scale, from very thin to very obese. Tailhead and rib (between the 12nd and 13th ribs) SFT was evaluated monthly by ultra-sonography. SFT was captured through Image Pro-Plus 4.5. Mare weights were estimated monthly with a specific tape. Embryo recovery rates (ER) and 30, 60 and 90 day recipient mare (n=174) pregnancy rates (PR) were compared by chi square. Data were submitted to the mixed procedure (SASÂ) and lsmeans were used. Age, BCS and time effects on dominant follicular diameter were analyzed by the analyses of variance (GLM, SASÂ). Correlations were analyzed by the PROC CORR (SASÂ), and regression by PROC REG. BCS was higher in older mares (P=0.02) and increase with time in experiment (P=0.009), and there was a positive interaction between weight and time (P<0.0001). Weight was not associate with age, but increased throughout the experiment (P=0.03). Tail (P=0.04) and rib (P=0.03) BCS increased with age (P<0.0001), and decreased (P=0.02) with time. There was a positive correlation between BCS and tail and rib SFT (P<0.0001). Dominant follicle diameter increased along the estrous cycle (P<0.0001) and was smaller (P<0.05) for mares with BCS below 6.5. Time but not age influence dominant follicle diameter (P<0.01). Mean ovulatory follicle diameter was 38.43 Â 3.09. ER was not affected by age or BCS but was lower (41%, P=0.003) in mares that ovulated follicles smaller than 40mm compared to mares ovulating follicles greater than 40mm (59%). Donor age was not associated with recipient pregnancy rates. It is concluded that the embryo recovery rate and ovulatory dominant follicle diameter were associated to body fat deposition; moreover, body condition scoring was not a good predictor of subcutaneous fat depth under the present conditions, since it decreased with time in experiment, with increased physical activity. Thus, it seems that ultra-sonography may be essential for correct and reliable body condition scoring, especially for horses under marching exercise management.

Foi comparado o julgamento do escore de condiÃÃo corporal (ECC) com a espessura de gordura subcutÃnea (EGS) e sua relaÃÃo com a eficiÃncia reprodutiva de Ãguas Mangalarga Marchador doadoras de embriÃo. O experimento foi conduzido com 56 Ãguas doadoras vazias entre os meses de agosto de 2008 e abril de 2009. O ECC, avaliado mensalmente, foi obtido pela mÃdia de dois avaliadores dentro de uma escala de 1 (extremamente magro) atÃ 9 (extremamente obeso). A EGS na regiÃo de inserÃÃo da cauda e entre a 12Â e 13Â costelas foi avaliada mensalmente com ultrassom e mensurada por meio do programa Image Pro-Plus 4.5. O peso foi estimado mensalmente com fita prÃpria de…

Advisors/Committee Members: Eduardo Pinto Filgueiras, JosÃ Augusto de Freitas Lima, Nadja Gomes Alves, JosÃ CamisÃo de Souza, Adalgiza Souza Carneiro de Rezende.

Subjects/Keywords: reproduction; transferÃncia de embriÃes; balanÃo energÃtico; ZOOTECNIA; embryo transfer; energy balance; reproduÃÃo; equine; equino

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodrigues, P. G. (2009). Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rodrigues, Paula Gomes. “Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares.” 2009. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed February 27, 2021. http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rodrigues, Paula Gomes. “Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares.” 2009. Web. 27 Feb 2021.

Vancouver:

Rodrigues PG. Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. [cited 2021 Feb 27]. Available from: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=2674.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rodrigues PG. Comparison between body condition scoring and subcutaneous fat thickness and its relation to reproductive efficiency in Mangalarga Marchador donor mares. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2009. Available from: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=2674

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

21. Vinicio Araújo Nascimento. Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas.

Degree: 2009, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2363

► The objective was to evaluate follicular dynamics and reproductive efficiency in Nelore cows with the use of estradiol benzoate (EB) on the synchronization of ovulation… (more)

▼ The objective was to evaluate follicular dynamics and reproductive efficiency in Nelore cows with the use of estradiol benzoate (EB) on the synchronization of ovulation associated to injectable amino acids and polypeptides supplementation; verify follicular dynamics and reproductive efficiency of hormonal protocols on the synchronization of ovulation using pFSH for Timed Artificial Insemination (TAI) in Nelore cows; verify follicular dynamics and reproductive efficiency when using gonadotropic stimulating hormones (eCG and pFSH) in protocols of synchronization of ovulation in Girolando cows; to evaluate follicular dynamics and reproductive efficiency with the use of EB associated to recombinant bovine somatotropin (bST-r) on the synchronization of ovulation protocols in Girolando cows and to study the efficiency of association of nutritional flushing on the superovulatory response, number, quality and morphology of embryos in Nelore cows. Five studies were conducted.The statistical analysis was performed using SAS 9.0 program with 5% of probability. In the Study I, with the Experiment I, 32 Nelore cows were evaluated by follicular dynamics in a 2X2 factorial arrangement, receiveing amino acids and polypeptides (Tsupplementation) or no (Tcontrol). Also, two protocols of synchronization of ovulation were used: TEB9, with EB administration on days 0 and 9 and TAI performed at 50-56 h after progesterone device removal; and TEB8, similar to the first one, but with the second dose of EB on day 8, and TAI performed at 48h after its removal. In the experiment II, reproductive efficiency was evaluated in Tcontrol (n=48), Tsupplementation (n=52), TEB8 (n= 51) and TEB9 (n=49). In the experiment I, there were no effects (P>0.05) of the synchronization protocols or of the supplementation on the follicular dynamics. The preovulatory follicle diameter was 11.06; 10.36; 11.25; 10.18 mm for cows in Tcontrol, Tsupplementation, TEB8 and TEB9, respectively. In the experiment II, there was no difference (P>0.05) for pregnancy rate after TAI, after natural mating or accumulated for Tcontrol= (56.25; 38.09; 72.92%), Tsupplementation (50.00; 65.38; 80.77%), TEB8 (54.90; 52.17; 78.43%) and TEB9 (51.02; 62.00; 75.51%). The administration of injectable amino acids and polypeptides supplementation and the interval from EB administration to TAI did not influence follicular dynamics, the interval to ovulation and the pregnancy rates after TAI, after natural mating and accumulated. In the Study II, with the experiment I, follicular dynamics was evaluated using 30 cows in three treatments: 1) TeCG (n=10) - on day 0, insertion of intravaginal progesterone devices plus 2 mg benzoate of estradiol (BE), intramuscularly (IM); on day 8, devices removal and 300 IU eCG IM, 0.15 mg PGF2α and 1 mg EB; on day 10, AI was performed 48 h after devices removal; 2) TFSH48 (n=10) - similar to previous treatment, but instead of eCG, 10 mg NIH FSH-S1 (FSH-p) was done; TFSH42 (n=10) - similar to TFSH48, but AI was performed 42 h after devices removal. In the… Advisors/Committee Members: Giovanni Ribeiro de Carvalho, Thea Mirian Medeiros Machado, Clóvis Andrade Neves, Margarida Maria Nascimento Figueiredo de Oliveira, Ciro Alexandre Alves Torres.

Subjects/Keywords: Reprodução; Bovino; Inseminação artificial; Transferência de embriões; PRODUCAO ANIMAL; Bovine; Reproduction; Artificial insemination; Embryo transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nascimento, V. A. (2009). Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nascimento, Vinicio Araújo. “Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas.” 2009. Thesis, Universidade Federal de Viçosa. Accessed February 27, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nascimento, Vinicio Araújo. “Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas.” 2009. Web. 27 Feb 2021.

Vancouver:

Nascimento VA. Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas. [Internet] [Thesis]. Universidade Federal de Viçosa; 2009. [cited 2021 Feb 27]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nascimento VA. Inseminação artificial em tempo fixo e transferência de embriões na eficiência reprodutiva de fêmeas bovinas. [Thesis]. Universidade Federal de Viçosa; 2009. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

22. Rafael Guedes Goretti. Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino.

Degree: 2005, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=274

► The aim of the present study was determine the soluble uterine fluid protein concentration of Mangalarga Marchador pregnant and non pregnant mares, at the embryo… (more)

▼ The aim of the present study was determine the soluble uterine fluid protein concentration of Mangalarga Marchador pregnant and non pregnant mares, at the embryo collection day, and correlate those with ultrasonic and palpable characteristics of the female genital tract (Chapter I). Was still studied the relation between serum progesterone concentration and soluble protein in uterine fluid concentration, analyzing the embryo recovery rate and quality and the pregnancy rate in recipient mares of embryos collected 48 h after luteolitic application. The interovulatory period and the luteolitic-ovulation interval were also observed (Chapter II). Twenty four estrous cycles of 12 embryo donors mares had a small amount of uterine fluid harvested at the embryo collection day (day 8 and 9 of the estrous cycle) which was submitted to the Bicinchoninic Acid Test. Ultra-sonographic and palpable characteristics of the uterus, cervix and corpus luteum were studied to establish correlations with pregnant and non pregnant mares. The presence of an embryo in the uterine flushing differentiated pregnant of non pregnant mares. The embryo recovery rate was 58.3 %. The mean protein concentration of uterine fluid of pregnant mares was 36.022.04 mg/mL and of non pregnant mares 42.2719.71 mg/mL, with no difference between groups (p>0.05). Additionally, no difference was observed between groups for uterine and corpus luteum ecogenicity, corpus luteum diameter and cervix and uterine tonus. In Chapter II, twenty estrous cycles of ten mares were monitored. Standard protocol of equine embryo transfer was applied to the first estrous cycle (luteolitic application at the day of embryo transfer, control group). In the second estrous cycle, luteolitic application was performed 48 h before embryo transfer (treated group). No difference (p>0.05) was observed in recovery rate, pregnancy in the recipient mare, embryo quality, protein concentration in uterine fluid and luteolitic-ovulation interval. In the control mares 80% of positive uterine flushes were observed, with seven pregnancies in recipient mares (87.5 %). In the treated animals the recovery rate was 70 %, with six pregnant recipient mares (85.7 %). The serum progesterone concentration was only determined for the treated group, being 13.865.42 ng/mL in the day of luteolitic application (48 h before embryo collection), decreasing to 0.460.25 ng/mL on the day of embryo collection (p<0.05). The uterine protein fluid concentration in the treated animals was 37.779.87 mg/mL and in the control ones 33.515.48 mg/mL (p>0.05). The interovulatory period was 18.5 days in the control mares and 25.9 in the treated ones, showing difference between themselves (p<0.05). Fetal-maternal interaction at days 8 and 9 after ovulation is not strong enough to change the parameters studied between pregnant and non pregnant mares. Many factors influence the soluble uterine protein concentration besides the embryo presence, which turns it into a very variable and inefficient parameter to determine viable conditions to… Advisors/Committee Members: Giovanni Ribeiro de Carvalho, Eduardo Paulino da Costa, Ciro Alexandre Alves Torres, Alan Maia Borges, José Domingos Guimarães.

Subjects/Keywords: Útero; Reprodução animal; Equino; Transferência de embriões; REPRODUCAO ANIMAL; Equine; Embryo transfer; Uterus; Animal reproduction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goretti, R. G. (2005). Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goretti, Rafael Guedes. “Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino.” 2005. Thesis, Universidade Federal de Viçosa. Accessed February 27, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goretti, Rafael Guedes. “Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino.” 2005. Web. 27 Feb 2021.

Vancouver:

Goretti RG. Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino. [Internet] [Thesis]. Universidade Federal de Viçosa; 2005. [cited 2021 Feb 27]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goretti RG. Colheita de embriões eqüinos 48 h após a aplicação de luteolítico: parâmetros reprodutivos, concentração sérica de progesterona e de proteínas do fluido uterino. [Thesis]. Universidade Federal de Viçosa; 2005. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

23. Bruna Waddington de Freitas. Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas.

Degree: 2010, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2859

►

O objetivo do presente estudo foi avaliar os parâmetros reprodutivos e fisiológicos de garanhões da raça Mangalarga Marchador, nas diferentes estações climáticas criados no município… (more)

▼

O objetivo do presente estudo foi avaliar os parâmetros reprodutivos e fisiológicos de garanhões da raça Mangalarga Marchador, nas diferentes estações climáticas criados no município de Viçosa – MG e mantidos em regime semi-estabulado. O estudo foi conduzido por um período de dez meses (abril de 2009 a março de 2010) utilizando três garanhões com idades entre 7 e 15 anos que foram submetidos a coletas de sêmen, em intervalos de 14 dias, para avaliação de parâmetros físicos e morfológicos, testes complementares e resfriabilidade do mesmo e quantificação de proteínas solúveis totais presentes no plasma seminal. Os parâmetros fisiológicos avaliados foram a freqüência cardíaca, respiratória e temperatura retal em intervalos semanais pela manhã e à tarde. Foram mensuradas as biometrias e ecotexturas testiculares antes das coletas de sêmen e, para determinação da testosterona sérica, foi realizada uma sessão de coleta de sangue a cada estação climática, com coletas em intervalos de 20 minutos por um período de 24 horas. Quanto às características seminais, observou-se um aumento progressivo do volume com e sem gel entre o outono e verão (p <0,05), enquanto a concentração do ejaculado e motilidade espermática foram menores no período primavera/verão (p <0,05). A porcentagem de espermatozóides com membrana celular lesada, avaliada pela fluorescência, indicou menores valores no outono e inverno, tanto para o sêmen fresco quanto para 24 horas pós-resfriamento (p <0,05). A concentração de proteínas solúveis totais presentes no plasma seminal não variou (p >0,05) entre as estações climáticas e apresentou uma média de 14,3 mg/mL. A secreção de testosterona não apresentou um padrão circadiano, porém as maiores concentrações ocorreram de 10:00 às 14:00, sendo que as maiores concentrações foram observadas no inverno e verão (p <0,05). O volume testicular não foi influenciado pela sazonalidade (p >0,05) e a ecotextura testicular foi maior no verão (p <0,05). Concluiu-se que a estação do ano exerce efeito sobre algumas características seminais, principalmente quanto aos aspectos físicos do sêmen e resistência ao processo de resfriamento do mesmo, além da ecotextura testicular e da secreção média de testosterona, de forma que a influência sobre estas últimas não apresentou correlações importantes com as demais características estudadas.

The aim of this study was to evaluate the reproductive and physiological parameters of Mangalarga Marchador stallions raised in Viçosa, Minas Gerais, Brazil during the four seasons and maintained semi-stabled. Thus, the study was conducted in a period of ten months (April 2009 to March 2010), using three stallions between 7 and 15 years of age and submitted to semen collections at intervals of 14 days for evaluation of physical and morphological semen parameters, complementary tests and cooling, and quantification of total soluble proteins present in seminal plasma. The physiological parameters measured were: respiratory and cardiac frequencies and rectal temperature at weekly intervals in the…

Advisors/Committee Members: Giovanni Ribeiro de Carvalho, Lincoln da Silva Amorim, Ciro Alexandre Alves Torres, Tarcízio Antônio Rego de Paula, Eduardo Paulino da Costa, José Domingos Guimarães.

Subjects/Keywords: Transferência de embriões; Progesterona; REPRODUCAO ANIMAL; Transition period; Embryo transfer; Progesterone; Período de transição

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Freitas, B. W. d. (2010). Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Freitas, Bruna Waddington de. “Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas.” 2010. Thesis, Universidade Federal de Viçosa. Accessed February 27, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Freitas, Bruna Waddington de. “Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas.” 2010. Web. 27 Feb 2021.

Vancouver:

Freitas BWd. Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas. [Internet] [Thesis]. Universidade Federal de Viçosa; 2010. [cited 2021 Feb 27]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Freitas BWd. Aspectos fisiológicos e seminais de garanhões da raça Mangalarga Marchador na região da Zona da Mata Mineira no decorrer das estações climáticas. [Thesis]. Universidade Federal de Viçosa; 2010. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=2859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Federal de Viçosa

24. Marcelo Milagres Rosado. Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro.

Degree: 2010, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4498

► Com o objetivo de estudar o efeito de diferentes protocolos de sincronização de estro (Uso de cloprostenol no momento da colocação do implante intravaginal e… (more)

▼ Com o objetivo de estudar o efeito de diferentes protocolos de sincronização de estro (Uso de cloprostenol no momento da colocação do implante intravaginal e sua permanência por período de oito e nove dias) sobre a taxa de prenhez em receptoras bovinas Bos taurus indicus x Bos taurus taurus, inovuladas com embriões de PIV, o presente estudo utilizou 1933 receptoras (3.649 tratamentos) divididas em 6 protocolos. No protocolo 1, as receptoras receberam 2,0 mg de Benzoato de estradiol (BE) e 500 μg de cloprostenol, e um dispositivo intravaginal (1,9 g de Progesterona) que permaneceu por 8 dias. No momento da retirada do dispositivo intravaginal cada receptora recebeu uma dose única de 0,5 mg de Cipionato de estradiol (CE), 500 μg de Cloprostenol e 400 UI de gonadotrofina coriônica eqüina (eCG). No protocolo 2, as receptoras receberam o mesmo tratamento que o primeiro grupo, porém sem a dose de 500 μg de Cloprostenol na colocação do dispositivo intravaginal de progesterona. No protocolo 3, as receptoras receberam no momento da colocação do dispositivo intravaginal de progesterona uma dose única de 500 μg de Cloprostenol e 2,0 mg de BE, sendo que o dispositivo permaneceu por 9 dias. Dois dias antes da retirada do dispositivo intravaginal (dia 7) as fêmeas receberam uma dose única de 500 μg de Cloprostenol, e no momento da retirada do implante, receberam uma dose única de 0,5 mg de CE e 400 UI de eCG. No protocolo 4, as receptoras receberam o mesmo tratamento que o protocolo 3, porém sem Cloprostenol na colocação do dispositivo intravaginal de progesterona. No protocolo 5, as receptoras receberam 2,0 mg de Benzoato de estradiol, e um dispositivo intravaginal de progesterona por 9 dias. Dois dias antes da retirada, no dia 7, receberam 500 μg de Cloprostenol e no momento da retirada do implante 0,5mg de CE e 300 UI de eCG. No protocolo 6, as receptoras receberam 2,0 mg de BE, e um dispositivo intravaginal de progesterona que permaneceu por 8 dias. No momento da retirada do dispositivo intravaginal, cada receptora recebeu 0,5 mg de CE, 500 μg de Cloprostenol e 300 UI de eCG. Todas as receptoras que apresentaram corpo lúteo foram inovuladas em média 10 dias após a retirada do dispositivo, ou seja, por volta de 8 dias após estro; e avaliadas por meio de ultrassonografia aos 58 dias após inovulação para o diagnóstico de gestação. Os dados foram submetidos a análises estatísticas descritivas (distribuição de freqüência) e os dados qualitativos foram arranjados em tabelas de contingência e analisados pelo teste de qui-quadrado a 5 % de probabilidade de erro. As receptoras do quarto protocolo apresentaram as melhores (p<0,05) taxas de aproveitamento (84,9%). No entanto, o número de tratamentos realizados (n=86) para o protocolo 4 foi reduzido em relação aos demais protocolos, mais estudos tornam-se necessários para confirmar a eficácia desse protocolo. Receptoras que receberam PGF2α 48 horas antes da retirada do dispositivo apresentaram melhores índices de aproveitamento de receptoras… Advisors/Committee Members: Ciro Alexandre Alves Torres, Eduardo Paulino da Costa, José Domingos Guimarães, Tarcízio Antônio Rego de Paula, Claudio José Borela Espeschit, João Henrique Moreira Viana.

Subjects/Keywords: Bovinos, Transferência de Embriões, PIV, CIDR; REPRODUCAO ANIMAL; Cattle; CIDR; Embryo transfer; in vitro fertilization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rosado, M. M. (2010). Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4498

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rosado, Marcelo Milagres. “Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro.” 2010. Thesis, Universidade Federal de Viçosa. Accessed February 27, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4498.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rosado, Marcelo Milagres. “Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro.” 2010. Web. 27 Feb 2021.

Vancouver:

Rosado MM. Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro. [Internet] [Thesis]. Universidade Federal de Viçosa; 2010. [cited 2021 Feb 27]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4498.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rosado MM. Uso de protocolo de sincronização de estro em receptoras mestiças inovuladas com embriões in vitro. [Thesis]. Universidade Federal de Viçosa; 2010. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=4498

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Colorado State University

25. Rasmussen, Sara-Lesley. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.

Degree: MS(M.S.), Biomedical Sciences, 2011, Colorado State University

URL: http://hdl.handle.net/10217/47443

► This study compared the pregnancy rates between embryo transfer of bovine embryos produced in vitro with sexed vs control sperm and artificial insemination (AI) using… (more)

Subjects/Keywords: assisted reproduction technologies; dairy cattle fertility; embryo transfer; IVF; pregnancy rates; sexed sperm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rasmussen, S. (2011). Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/47443

Chicago Manual of Style (16^th Edition):

Rasmussen, Sara-Lesley. “Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.” 2011. Masters Thesis, Colorado State University. Accessed February 27, 2021. http://hdl.handle.net/10217/47443.

MLA Handbook (7^th Edition):

Rasmussen, Sara-Lesley. “Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.” 2011. Web. 27 Feb 2021.

Vancouver:

Rasmussen S. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10217/47443.

Council of Science Editors:

Rasmussen S. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/47443

26. Burckhardt, Heather Ann. Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes.

Degree: MS, Laboratory Animal Medicine, 2009, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-1140

► Surgical embryo transfer in rodents is a common procedure in today’s research laboratory, although little is known of the effect analgesics may have on not… (more)

Subjects/Keywords: analgesics; mice; embryo transfer; pain

…history of embryo transfer occurred in 1956, with a paper written by Anne McLaren and Donald… …other time-point combinations. These findings have been incorporated into embryo transfer… …embryo transfer recipient mice that became pregnant (18). In “dummy” transfers, where… …5 1.2 TARGETED MUTANT MOUSE PROJECT Little has changed in embryo transfer from the… …genes from an entirely new species into a genome (8, 28). Embryo transfer is of…

8 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burckhardt, H. A. (2009). Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-1140

Chicago Manual of Style (16^th Edition):

Burckhardt, Heather Ann. “Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes.” 2009. Masters Thesis, Texas A&M University. Accessed February 27, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-1140.

MLA Handbook (7^th Edition):

Burckhardt, Heather Ann. “Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes.” 2009. Web. 27 Feb 2021.

Vancouver:

Burckhardt HA. Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes. [Internet] [Masters thesis]. Texas A&M University; 2009. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1140.

Council of Science Editors:

Burckhardt HA. Incorporation of analgesics into rodent embryo transfer protocols: assessing the effects on reproductive outcomes. [Masters Thesis]. Texas A&M University; 2009. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-1140

Massey University

27. Greaney, Kenneth Barry. A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep.

Degree: MS, Animal Science, 1991, Massey University

URL: http://hdl.handle.net/10179/5350

► The overall objective of this trial was to compare embryo transfer results from two seasonal periods (out-of-season vs in-season) in order to determine the effect… (more)

▼ The overall objective of this trial was to compare embryo transfer results from two seasonal periods (out-of-season vs in-season) in order to determine the effect of the season on the different parameters of reproductive performance such as; theincidence of oestrus, ovarian response to exogenous gonadotrophins, fertilisation rates and the number of lambs born per donor ewe programmed and flushed. Five breeds of imported sheep (Danish Texel {DT}, Finnish Texel {FT}, Gotland Pelt {GOT), Oxford Down {OXD}, and the White Headed Marsh {WHM}) consisting of two age groups (14-16 month-old and 26-28 month-old) in a commercial embryo transfer programme (LambXL, Manawatu) were used. A total of 553 ewes out-of-season and 234 ewes in-season were studied. Two data sets were selected from these seasonal groups; (1) The random data set. (2) The repeat data set, which consisted of the same donor ewes in each seasonal group. Oestrous synchronisation was attempted with a double CIDR-G regime and a super-ovulatory treatment consisting of an initial PMSG injection (200-300IU) and a series of six descending doses of FSH-P (total dose 24-36mg). The ewes were inseminated intra-uterine with fresh diluted semen from a ram of the same breed on the basis of oestrous detection. Embryo recovery was attempted on day 6.5-7 after oestrous detection using a laparoscope-aided uterine flush technique. Two embryos were transferred to each synchronised recipient ewe within two hours of recovery. The incidence of oestrus for the out-of-season and in-season groups was 93.3% and 100%, for the random data set compared to 93.9% and 100% for the repeat data set, respectively. The ovulatory response to the super-ovulatory treatments was significantly affected by the interaction of the breed and age of the donor in the random data set, but the repeat data set ovulation rate was not significantly affected by any of the variables recorded in this study. This interaction was attributable to the GOT breed having a higher ovulation rate in the older age group relative to the younger age group which was the reverse trend exhibited by the remaining breeds. However, there was an overall tendency for the out-of-season ovulation rate to be higher than that in-season, 7.64CL vs 6.60CL for the random data set and a difference (out-of-season - in-season) of +2.86CL was recorded for the repeat data set. The embryo recovery rates were 53.4% out-of-season and 53.5% in-season for the random data set and a difference of -0.7 percentage points was recorded for the repeat data set. The fertilisation rate was not significantly atfected by the season with 75.5% out-of-season and 65.7% in-season from the random data set and a difference of -4.5 percentage points was recorded for the repeat data set. The yield of good quality transferable embryos was significantly affected by the season with 78.2% out-of-season and 83.7% in-season from the random data set but the repeat data set was not significantly affected by the season with a difference of -11.0 percentage points. The embryo…

Subjects/Keywords: Embryo transfer; Sheep embryos; Sheep breeding

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Greaney, K. B. (1991). A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep. (Masters Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/5350

Chicago Manual of Style (16^th Edition):

Greaney, Kenneth Barry. “A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep.” 1991. Masters Thesis, Massey University. Accessed February 27, 2021. http://hdl.handle.net/10179/5350.

MLA Handbook (7^th Edition):

Greaney, Kenneth Barry. “A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep.” 1991. Web. 27 Feb 2021.

Vancouver:

Greaney KB. A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep. [Internet] [Masters thesis]. Massey University; 1991. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10179/5350.

Council of Science Editors:

Greaney KB. A study of the results of an embryo transfer programme conducted during two seasonal periods using five imported breeds of sheep. [Masters Thesis]. Massey University; 1991. Available from: http://hdl.handle.net/10179/5350

28. Vieira, Lais Mendes. Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical.

Degree: Mestrado, Reprodução Animal, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-29042013-103039/ ;

► A alta variabilidade na resposta aos tratamentos superovulatórios e na produção de embriões tem sido relacionada a causas multifatoriais. Além disso, são restritas as informações… (more)

▼ A alta variabilidade na resposta aos tratamentos superovulatórios e na produção de embriões tem sido relacionada a causas multifatoriais. Além disso, são restritas as informações que relacionam fatores correlatos ao embrião à concepção. Dessa forma, o objetivo do presente estudo foi avaliar a influência de variáveis relacionadas às doadoras (categoria animal e época do ano de produção do embrião) na eficiência de programas de superovulação (SOV), assim como na eficiência reprodutiva de receptoras em programas de transferência de embrião. Foram avaliados dados de 1.562 protocolos de SOV (609 em vacas em lactação e 953 em novilhas da raça Holandesa) e 4.076 transferências de embrião (receptoras Holandesas em lactação). A taxa de SOV (número de doadoras com dois ou mais CL) foi semelhante entre novilhas e vacas em lactação (89,7 vs. 91,9%, respectivamente; P = 0,26). A época do ano, também, não influenciou a taxa de SOV (época quente = 89,3% vs. época não quente = 92,2%; P = 0,09). Doadoras lactantes apresentaram maior número de CL (10,6 ± 0,6 vs. 7,5 ± 0,4; P < 0,0001), de estruturas recuperadas (7,6 ± 0,6 vs. 4,6 ± 0,4; P < 0,0001) e taxa de recuperação (77,6 vs. 58,7%; P < 0,0001) que novilhas. Doadoras superovuladas na época quente apresentaram menor número de CL (8,3 ± 0,5 vs. 9,7 ± 0,5; P = 0,03), de estruturas recuperadas (5,3 ± 0,5 vs. 6,7 ± 0,5; P = 0,04) e taxa de recuperação (65,7 vs. 72,3 %; P = 0,007) que doadoras superovuladas na época não quente. A taxa de fertilização (47,9 vs. 82,4%; P < 0,0001), de embriões viáveis (31,5 vs. 67,4%; P < 0,0001) e embriões viáveis grau I e II (15,4 vs. 42,1%; P < 0,0001) foram inferiores em doadoras lactantes. Semelhantemente, inferiores taxas de embriões viáveis (44,6 vs. 54,0%; P = 0,002) e embriões viáveis grau I e II (21,4 vs. 32,8%; P < 0,0001) foram verificadas durante a época quente do ano. No entanto, a queda na taxa de embriões grau I e II durante a época quente foi mais acentuada em doadoras lactantes (21,7 e 10,7%), quando comparada à observada em novilhas (46,2 e 38,1%; P = 0,02). Apesar da época do ano não ter influenciado a taxa de fertilização (época quente = 64,9 vs. época não quente = 70,0%; P = 0,07), foi observado relação negativa com a temperatura máxima média durante os 15 dias do protocolo de SOV (P = 0,006). A taxa de embriões viáveis também se mostrou negativamente relacionada com a temperatura máxima durante os 15 dias do protocolo de SOV (P = 0,03). O número de embriões viáveis foi semelhante entre as categorias (novilhas = 3,8 ± 0,3 vs. vacas em lactação = 3,3 ± 0,4; P = 0,16), porém foi inferior quando produzido durante a época quente (2,8 ± 0,3 vs. 4,4 ± 0,4; P = 0,03). A taxa de concepção dos embriões oriundos de doadoras em lactação foi maior aos 31 (36,0 vs. 30,7%; P = 0,001) e aos 45 dias de gestação (28,3 vs. 23,1%; P = 0,001). No entanto, a categoria da doadora não influenciou a perda gestacional (novilhas = 18,2% vs. vacas em lactação = 16,6%; P = 0,49). Ainda, as taxas de concepção aos 31 dias (30,5 vs. 31,7%: P = 0,47) e aos 45 dias… Advisors/Committee Members: Baruselli, Pietro Sampaio.

Subjects/Keywords: In vivo embryo production; Doadoras Holandesa; Embryo transfer; Estresse térmico; Heat stress; Holstein donors; Produção in vivo de embriões; Superovulação; Superovulation; Transferência de embriões

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vieira, L. M. (2013). Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-29042013-103039/ ;

Chicago Manual of Style (16^th Edition):

Vieira, Lais Mendes. “Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical.” 2013. Masters Thesis, University of São Paulo. Accessed February 27, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-29042013-103039/ ;.

MLA Handbook (7^th Edition):

Vieira, Lais Mendes. “Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical.” 2013. Web. 27 Feb 2021.

Vancouver:

Vieira LM. Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Feb 27]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-29042013-103039/ ;.

Council of Science Editors:

Vieira LM. Fatores associados à produção e à sobrevivência embrionária em programas de superovulação e transferência de embriões em fêmeas da raça Holandesa em clima tropical. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-29042013-103039/ ;

29. Gregoire, Anne. Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing.

Degree: Docteur es, Physiologie et biotechnologies de la reproduction, 2012, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2012LYO10168

►

La conservation de la biodiversité génétique du cochon d’Inde (Cavia porcellus) est importante en tant qu’animal de consommation endémique de la région andine ainsi qu’en… (more)

▼

La conservation de la biodiversité génétique du cochon d’Inde (Cavia porcellus) est importante en tant qu’animal de consommation endémique de la région andine ainsi qu’en tant qu’animal modèle précieux pour la recherche biomédicale.L’objectif de ce travail était de mettre en place les protocoles qui permettront une conservation ex situ des ressources génétiques de cette espèce par la voie femelle. Les étapes nécessaires à la concrétisation d’un projet de cryobanque sont :- la maîtrise de la production d’embryons grâce à des traitements hormonaux de synchronisation du cycle œstral et de superovulation des femelles,- la cryoconservation dans l’azote liquide des embryons obtenus et leur transfert dans des femelles receveuses.Une méthode standardisée de synchronisation des chaleurs basée sur l’administration d’altrenogest per os pendant 15 jours a été définie. Les chaleurs des femelles donneuses et receveuses d’embryons apparaissent dans les 4 à 5 jours qui suivent l’arrêt du traitement de ce progestagène.Des traitements de superovulation basés sur 3 injections à 24 heures d’intervalle d’hMG, ou 6 injections à 12 heures d’intervalle de FSH-recombinante humaine, permettent une augmentation du taux d’ovulation de l’ordre de fois 3 à fois 4 par rapport à des femelles non-traitées. Toutefois les réponses au traitement restent variables et il faudra réitérer l’expérience sur de plus grands lots d’animaux afin d’ajuster les doses et les moments d’application.La méthode de congélation lente, utilisant l’éthylène glycol comme cryoprotecteur, a permis d’obtenir des taux de survie embryonnaire satisfaisants (70,3%). La méthode de vitrification a également donné de bons résultats, avec un taux de survie embryonnaire de 41,7%.Le premier transfert au monde d’embryons frais réalisé avec succès dans une femelle receveuse préalablement synchronisée a été obtenu lors de ce travail. Les transferts d’embryons décongelés n’ont pas encore donné lieu à des gestations.

The preservation of the guinea pig (Cavia porcellus) genetic biodiversity is important as a native source of protein for many highlanders in the Andean region and as a precious laboratory animal for biomedical research.The aim of this work was to establish the protocols that will enable an ex situ preservation of the genetic resources of this species by the female way.The necessary steps for the achievement of a cryobank project are:- the control of the embryo production thanks to hormonal treatments to synchronize the estrous cycles and to superovulate the females,- the cryopreservation in liquid nitrogen of the obtained embryos and their transfer into recipient females.A standard method for synchronization of heat periods based on the per os administration of altrenogest during 15 days has been defined. The heat periods of the females, donors and recipients of embryos, appears 4 to 5 days after the end of the progestagen treatment. Superovulation treatments, based on 3 injections at 24-hour intervals of hMG or 6 injections at 12-hour intervals of human recombinant-FSH, lead…

Advisors/Committee Members: Joly, Thierry (thesis director).

Subjects/Keywords: Cochon d’Inde; Synchronisation des chaleurs; Superovulation; Cryoconservation des embryons; Congélation lente; Vitrification; Transfert embryonnaire; Guinea pig; Heat period synchronization; Superovulation; Embryo cryopreservation; Slow freezing; Vitrification; Embryo transfer; 571.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gregoire, A. (2012). Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2012LYO10168

Chicago Manual of Style (16^th Edition):

Gregoire, Anne. “Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing.” 2012. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed February 27, 2021. http://www.theses.fr/2012LYO10168.

MLA Handbook (7^th Edition):

Gregoire, Anne. “Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing.” 2012. Web. 27 Feb 2021.

Vancouver:

Gregoire A. Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2012. [cited 2021 Feb 27]. Available from: http://www.theses.fr/2012LYO10168.

Council of Science Editors:

Gregoire A. Cryoconservation des ressources génétiques chez le cochon d’Inde (Cavia porcellus) : production et congélation des embryons : Cryopreservation of guinea pig (Cavia porcellus) genetic resources : embryos production and freezing. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2012. Available from: http://www.theses.fr/2012LYO10168

30. Gibson, C.M.E. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.

Degree: 2019, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

► The pre-implantation period is a critical time for the establishment and maintenance of pregnancy and, in the mare as in other species, is characterized by… (more)

▼ The pre-implantation period is a critical time for the establishment and maintenance of pregnancy and, in the mare as in other species, is characterized by a high incidence of embryonic loss. The equine conceptus develops for an usually long period within the uterine cavity (40-42 days) before implantation begins and a stable attachment is formed. Moreover, at the time of implantation, the equine embryo has already undergone a number of critical developmental events such as gastrulation, neurulation and initial organogenesis to reach the fetal stage. During its prolonged pre-implantation period, the equine conceptus is entirely dependent on the uterine luminal environment and, in particular, on the uterine secretions or ‘histotroph’ for nutrient provision. Since the luminal epithelium and endometrial glands synthesize, transport and/or secrete the histotroph into the uterine lumen, it is important to understand the mechanisms involved in regulating endometrial secretion of the molecules required for conceptus survival and development. The main focus of this thesis was to improve our understanding of the role of the uterine environment in supporting conceptus development and, in particular, to dissect the importance of maternal progesterone and conceptus-secreted factors in preparing the uterus for its role during pre-implantation development in the horse. To better understand the impact of the uterine environment on conceptus development, and to differentiate between the effects of progesterone and/or the embryo on endometrial gene expression we employed an equine asynchronous embryo transfer model. In this thesis, we show that the horse conceptus is sensitive to the uterine environment to which it is exposed and, in the case of uterine asynchrony, is able to adapt its development accordingly, in order to ensure subsequent roughly synchronous development. Reflecting this adaptability, we found that the endometrial transcriptome is subtly different in a uterus in the presence of a more advanced embryo (i.e. very few DEGs). By contrast, the conceptus is markedly affected (retarded) by a less advanced uterus; this is mainly a result of a shorter duration of endometrial exposure to progesterone, but presumably compounded by the influence of the conceptus itself as it succumbs to developmental retardation. The different gene expression profile of a uterus exposed to a shorter period of progesterone priming will result in a different histotrophic composition, which is presumably the primary signal/factor resulting in developmental retardation of the conceptus. Interestingly, the equine conceptus appears to have an unusual ability to not only sense the changes in the uterine environment but also to delay its development and wait for the endometrium to catch-up, thereby allowing the conceptus to survive. In this respect, it is possible that specific pathways (for example the kinin-kallikrein system) assist or enable the conceptus to detect the stage of the endometrium and regulate its development accordingly. Finally,… Advisors/Committee Members: Stout, Tom, de Ruijter - Villani, Marta.

Subjects/Keywords: conceptus; endometrium; pre-implantation period; asynchronous embryo transfer; horse; embryo development

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APA (6^th Edition):

Gibson, C. M. E. (2019). Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

Chicago Manual of Style (16^th Edition):

Gibson, C M E. “Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.” 2019. Doctoral Dissertation, University Utrecht. Accessed February 27, 2021. https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443.

MLA Handbook (7^th Edition):

Gibson, C M E. “Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.” 2019. Web. 27 Feb 2021.

Vancouver:

Gibson CME. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. [Internet] [Doctoral dissertation]. University Utrecht; 2019. [cited 2021 Feb 27]. Available from: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443.

Council of Science Editors:

Gibson CME. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. [Doctoral Dissertation]. University Utrecht; 2019. Available from: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

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