subject:(embryo implantation)

University of Newcastle

1. Roy, Tammie K. Matrix metalloproteinase-2 in early embryo implantation in the mouse.

Degree: PhD, 2011, University of Newcastle

URL: http://hdl.handle.net/1959.13/927259

►

Research Doctorate - Doctor of Philosophy (PhD)

In mammals the implantation of trophoblast cells into the uterus is highly invasive and requires the degradation of… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

In mammals the implantation of trophoblast cells into the uterus is highly invasive and requires the degradation of the extracellular matrix (ECM) of the uterine wall. It was first hypothesised more than 70 years ago that the invasiveness of the trophoblast cells was due to proteolytic activity. Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases that in combination are capable of degrading virtually all of the components of the ECM, both interstitial matrix and basement membrane [1, 2]. Research into implantation in rodent and ovine models has implicated numerous members of the MMP family (MMP-2, -3, -7, -9, -11, -13 and MT1-MMP) in the control of early events of embryo implantation [3-9]. The majority of these studies agree that the most important of the MMPs in implantation are MMP-2 and MMP-9. The process of embryo attachment and invasion is one of the most biologically complex processes studied in reproductive biology. While evidence exists for the presence of MMPs during embryo implantation, knowledge is limited on the exact expression patterns and regulatory pathways that control these enzymes and allow for the invasion of trophoblast cells in the maternal endometrium. Previous research, undertaken by this research group, has looked at the expression of MMP-2 and MMP-9 during embryo implantation in the mouse using a new model of mating that allows for a more accurate timing of implantation events. The results from these experiments showed that there is a significant increase in the presence of MMP-2 in the luminal flushes around 97 – 98 hours post-insemination (day 4 of pregnancy). The aim of this project was to test the broad hypothesis that MMP-2 proteins are expressed and activated at the early stages of blastocyst implantation by cells of both endometrial and embryonic origin. Additionally, studies also tested the hypothesis that the expression of MMP-2 is specific to the events of embryo implantation and requires an interaction between the embryo and endometrial cells. An optimised mating protocol was used to facilitate more accurate identification of the early stages of implantation in order to be able to further define the expression patterns of MMP-2. Immunohistochemical labelling of MMP-2 proteins in formalin fixed sections of embryo implantation sites at specific times post-insemination localised MMP-2 expression to cells of both the blastocyst and endometrium. Specifically, antibody staining was identified in trophectoderm cells and inner cell mass cells of the embryo and in endometrial epithelial cells of the lumen and in the cells of the endometrium. While these experiments demonstrated MMP-2 protein localisation, they did not allow the assessment proteinase activity. The presence of gelatinase activity was identified through the application of in situ zymography to fresh frozen sections of tissue from implantation sites collected at specific times post-insemination. Results from these experiments confirmed…

Advisors/Committee Members: University of Newcastle. Faculty of Health, School of Biomedical Sciences and Pharmacy.

Subjects/Keywords: matrix metalloproteinases; embryo implantation; mice

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roy, T. K. (2011). Matrix metalloproteinase-2 in early embryo implantation in the mouse. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/927259

Chicago Manual of Style (16^th Edition):

Roy, Tammie K. “Matrix metalloproteinase-2 in early embryo implantation in the mouse.” 2011. Doctoral Dissertation, University of Newcastle. Accessed April 18, 2021. http://hdl.handle.net/1959.13/927259.

MLA Handbook (7^th Edition):

Roy, Tammie K. “Matrix metalloproteinase-2 in early embryo implantation in the mouse.” 2011. Web. 18 Apr 2021.

Vancouver:

Roy TK. Matrix metalloproteinase-2 in early embryo implantation in the mouse. [Internet] [Doctoral dissertation]. University of Newcastle; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1959.13/927259.

Council of Science Editors:

Roy TK. Matrix metalloproteinase-2 in early embryo implantation in the mouse. [Doctoral Dissertation]. University of Newcastle; 2011. Available from: http://hdl.handle.net/1959.13/927259

University of Adelaide

2. Chin, Peck Yin. Cytokines and programming the pre-implantation embryo.

Degree: 2014, University of Adelaide

URL: http://hdl.handle.net/2440/92044

► As the pre-implantation embryo traverses the female reproductive tract, it experiences fluctuations in the composition of the surrounding maternal environment, including the availability of nutrients,… (more)

▼ As the pre-implantation embryo traverses the female reproductive tract, it experiences fluctuations in the composition of the surrounding maternal environment, including the availability of nutrients, growth factors and cytokines. In particular, the cytokine milieu surrounding the early embryo is pivotal in programming optimal embryo development. The pre-implantation embryo is sensitive to a range of perturbations such as maternal diet or in vitro culture. These and other insults influencing the maternal environment including infection, stress and environmental toxins may in part act via impact on oviduct and uterine cytokine synthesis. However the effect of maternal perturbation to inflammation or infection, on the embryo and the role of cytokines in mediating this is not fully elucidated. The studies described in this thesis employed an in vivo mouse model of maternal systemic inflammation with the proinflammatory bacterial lipopolysaccharide (LPS), where a pro-inflammatory cytokine response was elicited on days 2.5 and 3.5 post coitum (pc), prior to implantation. This model was studied in wildtype C57Bl/6 (Il10 ⁺ʹ⁺) mice and mice with a null mutation in the Il10 gene (Il10 ⁻ʹ⁻) were studied to investigate the effects of maternal deficiency in the anti-inflammatory cytokine IL-10 during LPS treatment. We demonstrated that the altered cytokine signals resulting from a low level pro inflammatory LPS challenge (0.5 μg/mouse) in the pre-implantation period elicit changes in the embryo developmental trajectory that in turn alter fetal growth and delay postnatal growth in the male progeny from LPS-treated mothers. As LPS did not directly impact development of the embryo at low and moderate doses, this result appears to reflect indirect effects of LPS mediated via the maternal tract. This is consistent with data from day 3.5 pc oviduct and uterus tissues which revealed increased mRNA expression of proinflammatory cytokines including Il6, Tnfa and Il12b following maternal LPS treatment. Peri-conceptional low dose LPS treatment in Il10 ⁺ʹ⁺ and Il10 ⁻ʹ⁻ mice revealed that the number of viable fetuses and fetal weight were both significantly reduced after LPS treatment, particularly in the Il10 ⁻ʹ⁻ mice. Embryo transfer was then utilised to investigate the mechanism by which LPS acts on the embryo, where day 3.5 pc embryos from donors treated with 0.5 μg LPS or PBS on days 2.5 and 3.5pc were transferred into day 2.5 pc pseudopregnant Swiss female recipients. The effect of maternal LPS treatment on fetal and placental development was seen to be maintained even after embryo transfer, suggesting that any effects of altered cytokine expression in embryos are exerted during cleavage stages before embryo recovery from donors. In addition, postnatal investigation of male and female progeny derived from control PBS and LPStreated Il10 ⁺ʹ⁺ and Il10 ⁻ʹ⁻ females from birth until 19 weeks of age showed that maternal LPS treatment constrains postnatal growth in male progeny regardless of maternal Il10 genotype, compared to male progeny… Advisors/Committee Members: Robertson, Sarah Anne (advisor), Thompson, Jeremy Gilbert E. (advisor), School of Paediatrics and Reproductive Health (school).

Subjects/Keywords: cytokines; pre-implantation embryo; inflammation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chin, P. Y. (2014). Cytokines and programming the pre-implantation embryo. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/92044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chin, Peck Yin. “Cytokines and programming the pre-implantation embryo.” 2014. Thesis, University of Adelaide. Accessed April 18, 2021. http://hdl.handle.net/2440/92044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chin, Peck Yin. “Cytokines and programming the pre-implantation embryo.” 2014. Web. 18 Apr 2021.

Vancouver:

Chin PY. Cytokines and programming the pre-implantation embryo. [Internet] [Thesis]. University of Adelaide; 2014. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2440/92044.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chin PY. Cytokines and programming the pre-implantation embryo. [Thesis]. University of Adelaide; 2014. Available from: http://hdl.handle.net/2440/92044

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

3. Green, Charmaine. THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION .

Degree: 2016, University of Sydney

URL: http://hdl.handle.net/2123/17684

► The use of assisted reproductive technologies (ART) such as in vitro fertilisation (IVF) is increasing and today close to 4 percent of babies born in… (more)

▼ The use of assisted reproductive technologies (ART) such as in vitro fertilisation (IVF) is increasing and today close to 4 percent of babies born in Australia, are as a result of ART. IVF procedures involve the culture of resultant embryos in medium that routinely lacks the growth factors that are present in the reproductive tract. Cultured embryos develop at a slower rate and have higher levels of developmental arrest, with fewer than 50% of embryos reaching the blastocyst stage. Furthermore, once an embryo is transferred back into the uterus, it faces the hurdle of implantation, with implantation failure being a major cause of IVF failure. This thesis examines whether the addition of growth factors to the embryo culture medium can increase blastocyst development and adhesion competency, using mouse embryos as a model system. In particular, the effect of insulin-like growth factor 1(IGF1) and insulin-like binding protein 3 (IGFBP3) on preimplantation mouse embryo development in vitro and the role of IGF1 on implantation in vitro was examined. In the present study, the culture of preimplantation embryos in the presence of a physiological concentration of IGF1 improved development from compaction onwards, resulting in improved blastocyst development. Conversely, high levels of IGF1 negatively impacted on development by decreasing hatching probably due to these high levels of IGF1 causing IGF1 receptor (IGF1R) down-regulation and apoptosis in the mouse embryo, as shown previously. Blocking the IGF1R with a neutralising antibody was shown to decrease blastocyst development, hatching and cell numbers and to increase apoptosis. Furthermore, treatment of blastocysts with IGF1 caused phosphorylation of Akt, which regulates cell survival by activating anti-apoptotic pathways. Therefore, IGF1 may act as a survival factor in the preimplantation embryo. During early implantation integrins accumulate on the surface of the blastocyst and endometrium and these interact with each other via extracellular matrix proteins such as fibronectin. These interactions are important for the attachment of blastocysts to the endometrium. In the present study treatment of blastocysts with IGF1 increased fibronectin on the surface of the blastocyst via activation of the Phosphoinositide 3 Kinase (PI3K) pathway. As a result, blastocysts had increased attachment to cultured uterine epithelial cells and increased outgrowth. In addition to IGF1, the reproductive tract produces IGFBP3, which is also thought to improve development of the embryo. However, to the best of our knowledge this is the first study to examine the effect of exogenous IGFBP3 on embryo development in vitro. IGFBP3 caused an increase rate of progression of embryos through the early stages of division (5-8cells) and activation of Akt and ribosomal protein S6 (rpS6) proteins as well as induction of calcium signalling. In the present study, it appears that IGFBP3 signalling in the embryo requires the IGF1R, as the use of an IGF1R neutralising antibody blocked IGFBP3 from enhancing…

Subjects/Keywords: IGF1 IGFBP3 Implantation Embryo Development

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Green, C. (2016). THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Green, Charmaine. “THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION .” 2016. Thesis, University of Sydney. Accessed April 18, 2021. http://hdl.handle.net/2123/17684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Green, Charmaine. “THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION .” 2016. Web. 18 Apr 2021.

Vancouver:

Green C. THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION . [Internet] [Thesis]. University of Sydney; 2016. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2123/17684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Green C. THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR SIGNALLING IN THE MOUSE EMBRYO DURING PREIMPLANTATION DEVELOPMENT AND EARLY IMPLANTATION . [Thesis]. University of Sydney; 2016. Available from: http://hdl.handle.net/2123/17684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

4. Aboussahoud, Wedad. THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION.

Degree: 2016, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:297944

► AbstractThe University of Manchester, Wedad Aboussahoud, Doctor of Medicine (MD)The Role of Toll-Like Receptors in Assisted Conception, June/2015Background In the last two decades the discovery… (more)

▼ AbstractThe University of Manchester, Wedad Aboussahoud, Doctor of Medicine (MD)The Role of Toll-Like Receptors in Assisted Conception, June/2015Background In the last two decades the discovery of the Toll-Like Receptor (TLR) family in humans revealed the importance of innate immunity in providing host defence against invading pathogens. Moreover, TLRs have a vital role in mediating the interactions between the reproductive and immune systems. Similar to TLRs, (NOD)-like receptors (NLRs); retinoic acid-inducible gene-1, RIG-1-like receptors (RLRs) are also important pattern recognition receptors in the female reproductive system. Successful implantation requires effective reciprocal interactions between receptive endometrium and competent embryo. The endometrial innate immunity during implantation has been intensively investigated. However, little is known about the expression of innate immunity during the early stages of human embryo development.Objective: To investigate the expression of innate immunity molecules during the early stages of human embryo development and to determine the functions of TLRs in blastocysts.Material and methodsThe expression of TLRs, a panel of downstream signalling molecules, NLRs, RLRs, inflammatory cytokines, chemokines and the hyaluronic acid system were investigated in the following developmental stages: oocyte, 4- cell, blastomeres, 8- cell, blastocyst, inner cell mass and trophectoderm using Affymetrix Human Genome U133 Plus 2.0 arrays. Microarray data were validated by Q-PCR. TLR function in human blastocysts was investigated by treating day five blastocysts with TLR3 or TLR5 specific ligands; Poly (I:C) and flagellin respectively, for 24 hours. The culture media was analysed for elevated cytokine and chemokine levels using cytometric bead array.ResultsTLRs, NLRs, RLRs, TLR downstream signalling molecules, cytokines and chemokines involved during implantation event and the hyaluronic system were all found to be positively expressed in the early stages of human embryo development. The expression levels of the above molecules were generally moderate to low (CT 24-34) and varied across the embryonic developmental stages. Stimulation of TLR3 and TLR5 in day 5 blastocysts produced cytokines and chemokines. In addition, there were alterations in gene expression patterns in the Poly (I:C) and flagellin treated blastocysts in comparison to the untreated blastocysts. ConclusionThe varied expression levels of the investigated molecules involved in early embryonic developmental suggests a potential role for these molecules in early pregnancy loss and implantation failure. Specifically, the relationship between the level of TLR expression, function and embryo quality is worth investigating in the future as a potential marker for embryo competence Advisors/Committee Members: BRISON, DANIEL D, Seif, Mourad, Brison, Daniel.

Subjects/Keywords: Toll Like Recepors; Human embryos; Embryo implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboussahoud, W. (2016). THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:297944

Chicago Manual of Style (16^th Edition):

Aboussahoud, Wedad. “THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION.” 2016. Doctoral Dissertation, University of Manchester. Accessed April 18, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:297944.

MLA Handbook (7^th Edition):

Aboussahoud, Wedad. “THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION.” 2016. Web. 18 Apr 2021.

Vancouver:

Aboussahoud W. THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2021 Apr 18]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:297944.

Council of Science Editors:

Aboussahoud W. THE ROLE OF TOLL-LIKE RECEPTORS IN ASSISTED CONCEPTION. [Doctoral Dissertation]. University of Manchester; 2016. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:297944

AUT University

5. Erikson, Elizabeth Kate Louise. In Vivo implantation Murine Modeling .

Degree: 2012, AUT University

URL: http://hdl.handle.net/10292/4734

► Ethical and legal restrictions limit human implantation knowledge. Alternative tools, such as retrospective clinical studies, in vitro three dimensional cell culture modelling, and in vivo… (more)

▼ Ethical and legal restrictions limit human implantation knowledge. Alternative tools, such as retrospective clinical studies, in vitro three dimensional cell culture modelling, and in vivo implantation animal modelling provide some insight. While in vivo implantation murine models have proven useful, none are recognised as standard. Therefore, the overall research objective was to gain an understanding of improved standardization of in vivo implantation murine models, suitable to explore implantation and preliminary studies surrounding In Vitro Fertilization (IVF) intervention potential. A selection of models, termed optimal or suboptimal by their level of implantation, were examined and compared to natural mouse pregnancy. Embryo transfer (ET) outcomes on day 17 post coitus (pc) and the ability of the most suitable optimal and suboptimal models to detect consequences of manipulated embryos, an IVF intervention, were explored. Significant inconsistencies within suboptimal models led to investigating the beginning of endometrial receptivity within natural pregnancy, and synchronous and asynchronous uterine ET. Assessed time points were set to capture the pre receptive and receptive endometrial phases. Examining at least three mice per time point, achieving 80% statistical power, ensured statistical robustness. This research extended current knowledge in three ways. Firstly, ETs performed at two hourly intervals, demonstrated significant influence of the distinct moments of coitus, ET and autopsy on implantation rate consistency. Previous research, which specified the day of transfer rather than hours pc, was unable to reveal such influence. Finer control of coitus, ET and autopsy demonstrated improved implantation rate consistency, necessary for model standardization. Implementation within wider research could improve statistical robustness and facilitate greater comparison between research groups. Secondly, assessed time points extended beyond previous the literature, which did not report endometrial receptivity within natural pregnancy prior to 92 hours pc. This study reported endometrial receptivity by 88 hours pc. No mean zero baselines were also observed, suggesting endometrial receptivity could begin prior to 88 hours pc. A four hour difference is significant, given murine endometrial receptivity is considered at least 12, but less than 24 hours long, translating to a potential 33% increase in known implantation opportunity. Data collection surrounding the beginning of endometrial receptivity within natural pregnancy, and synchronous and asynchronous uterine ET, revealed reasons for implantation rate inconsistencies, not previously observed in single-point data sets; contributing towards the first point. Thirdly, this is the first report of early endometrial receptivity, following the transfer of mature pre-implantation embryos directly into uteri, whose endometrium would not normally be exposed to embryos at that time. Only one other study, which performed oviductal transfers,… Advisors/Committee Members: Henry, Stephen (advisor), Blake, Debbie (advisor).

Subjects/Keywords: Implantation; Mouse; Iin vivo; Modeling; Mice; Murine; Embryo transfer; Uterus; Embryo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Erikson, E. K. L. (2012). In Vivo implantation Murine Modeling . (Thesis). AUT University. Retrieved from http://hdl.handle.net/10292/4734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Erikson, Elizabeth Kate Louise. “In Vivo implantation Murine Modeling .” 2012. Thesis, AUT University. Accessed April 18, 2021. http://hdl.handle.net/10292/4734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Erikson, Elizabeth Kate Louise. “In Vivo implantation Murine Modeling .” 2012. Web. 18 Apr 2021.

Vancouver:

Erikson EKL. In Vivo implantation Murine Modeling . [Internet] [Thesis]. AUT University; 2012. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10292/4734.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Erikson EKL. In Vivo implantation Murine Modeling . [Thesis]. AUT University; 2012. Available from: http://hdl.handle.net/10292/4734

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

6. Lu, Chiao-Hui. The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.

Degree: Master, Biological Sciences, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546

► Since Louise Joy Brown, the first baby of In Vitro Fertilization-Embryo Transfer (IVF-ET), was born in the United Kingdom in 1978, there had been over… (more)

▼ Since Louise Joy Brown, the first baby of In Vitro Fertilization-Embryo Transfer (IVF-ET), was born in the United Kingdom in 1978, there had been over five million successful cases over the course of 35 years. There are two commercially available culture media in the market: Single Step Medium (Global Medium) and Sequential Medium (Sage Medium). The development of these two media had enhanced the quality of embryo and increased the pregnancy rate in IVF-ET. In this collection of 210 cases of IVF-ET, oocytes were fertilized by intracytoplasmic sperm injectionï¼ICSIï¼and randomly assigned to the two commercially-available culture media. The quantity and quality of embryos were observed and recorded for three consecutive days in order to compare the development of embryos in the two commercial culture medium. A fertilized rate of 75.5% in Global Medium and 77.5% in Sage Medium were observed on the first day. There was a higher number of cleaved cells in Global Medium and embryos of Grade 1 cells than those in Sage Medium both on the 2nd and the 3rd day, though no significant differences were noted in the pregnancy rates between the two samples. In the group of those over 38 years old, there was a higher number of cells in Global Medium, but exhibited no difference in either embryonic pattern or in the pregnancy rate. Though the development of commercial culture medium had enabled a longer cultivation, some cases still failed be cultured to the blastocytic stage due to other limitations. It had been attempted with morphologic selection in embryos by the 3rd day of culture, with a criteria of over six cells per embryo and minimal cell fragmentation, to reach compatible pregnancy rate of embryo implantation of the 5th day by conventional method. Our study indicated that the there was higher number of Grade 1 embryos with more than 6 cells and lower embryo fragmentation in Single Step Medium, thus yielded a better overall quality of embryo than Sequential Medium. It can be speculated, therefore, that the Single Step Medium would offer a valuable choice in patients with difficulty in reaching blastocytic stage by conventional method due to various factors. Advisors/Committee Members: Lee Su-long (chair), Liu, Jong-Kang (committee member), Chang Chi-Chang (committee member), Chen, Chun-Lin (chair).

Subjects/Keywords: quality of embryo; embryo implantation; Sequential Medium; Single Step Medium; IVF; Embryo culture; In Vitro Fertilization

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lu, C. (2014). The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lu, Chiao-Hui. “The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.” 2014. Thesis, NSYSU. Accessed April 18, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lu, Chiao-Hui. “The pregnancy outcomes for human embryos cultured in single step medium and sequential medium.” 2014. Web. 18 Apr 2021.

Vancouver:

Lu C. The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Apr 18]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lu C. The pregnancy outcomes for human embryos cultured in single step medium and sequential medium. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0105114-012546

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

7. Burnum, Kristin Elizabeth. Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry.

Degree: PhD, Biochemistry, 2008, Vanderbilt University

URL: http://hdl.handle.net/1803/15001

► Molecular events involved in successful embryo implantation take place in defined time and space but are not generally well understood. Here for the first time,… (more)

Subjects/Keywords: Imaging Mass Spectrometry; Embryo Implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burnum, K. E. (2008). Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15001

Chicago Manual of Style (16^th Edition):

Burnum, Kristin Elizabeth. “Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry.” 2008. Doctoral Dissertation, Vanderbilt University. Accessed April 18, 2021. http://hdl.handle.net/1803/15001.

MLA Handbook (7^th Edition):

Burnum, Kristin Elizabeth. “Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry.” 2008. Web. 18 Apr 2021.

Vancouver:

Burnum KE. Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry. [Internet] [Doctoral dissertation]. Vanderbilt University; 2008. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1803/15001.

Council of Science Editors:

Burnum KE. Detecting spatial and temporal distributions of lipids and proteins during embryo implantation by MALDI Imaging Mass Spectrometry. [Doctoral Dissertation]. Vanderbilt University; 2008. Available from: http://hdl.handle.net/1803/15001

University of California – San Diego

8. Dumdie, Jennifer Nicole. Molecular Determinants of Oocyte and Embryo Developmental Competence.

Degree: Biomedical Sciences, 2019, University of California – San Diego

URL: http://www.escholarship.org/uc/item/0kq611t3

► The earliest stages of life, including the transition from the fully differentiated oocyte to the totipotent zygote, the first days of embryo cleavage and cell… (more)

▼ The earliest stages of life, including the transition from the fully differentiated oocyte to the totipotent zygote, the first days of embryo cleavage and cell differentiation to form a blastocyst, and implantation of that blastocyst in the wall of the uterus, are somehow beautifully simple and strikingly complex at the same time. These stages, which represent the beginning of life for us and other vertebrates, are difficult to study, owing to a number of experimental and ethical considerations. However, advances in our understanding of nuclear reprogramming, transcriptional quiescence and activation, the maintenance of pluripotency, and what it takes for an embryo to initiate contact with the endometrium to generate a successful pregnancy, will without question have far-reaching influence for many scientific and medical disciplines. Here, I attempt to unravel some of these concepts, combining molecular and developmental biology with genome-wide analysis only recently made possible through advances in techniques with low input. These approaches, in combination, allow us to ask questions about early developmental systems that would not have been possible only years prior.In the oocyte, global transcriptional silencing is a highly conserved mechanism that is essential for the transition from the differentiated oocyte to the totipotent zygote. Here, I report that global transcriptional silencing in the mouse oocyte depends on an mRNA decay activator. By downregulating master regulators of transcription during oocyte growth, particularly a group of mRNAs encoding demethylases for H3K4 and H3K9, ZFP36L2 enables increased histone methylation that is associated with transcriptional silencing. These results uncover a mRNA decay mechanism that acts a developmental switch during growth of the mammalian oocyte, resulting in wide-spread shifts in chromatin modification, and mediating silencing of transcription in the oocyte.The pluripotent population of cells in the blastocyst, the inner cell mass, is established in the mouse embryo approximately three days after fertilization. These cells will undergo gastrulation to form the entire organism and can be maintained in culture as embryonic stem cells. I report here that UPF2, a mRNA decay activator, is needed specifically within the pluripotent inner cell mass of the mouse embryo for maintenance of pluripotency in the embryo in vivo, as well as for embryonic stem cells in vitro. That mRNA decay may underly the establishment or maintenance of this intriguing and complex population of cells, is an exciting possibility.Reproductive success depends on embryo implantation in the uterus, and in fertile and infertile couples alike, failure of the embryo to implant in the wall of the uterus accounts for up to 75% of all lost pregnancies. Here, I provide a qualitative assessment of gene expression and cellular communication networks within the major compartments of the human blastocyst that are most closely associated with successful implantation and ongoing pregnancy. Most strikingly,…

Subjects/Keywords: Developmental biology; Bioinformatics; Blastocyst; Embryo; Implantation; Oocyte; RNA decay

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dumdie, J. N. (2019). Molecular Determinants of Oocyte and Embryo Developmental Competence. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/0kq611t3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dumdie, Jennifer Nicole. “Molecular Determinants of Oocyte and Embryo Developmental Competence.” 2019. Thesis, University of California – San Diego. Accessed April 18, 2021. http://www.escholarship.org/uc/item/0kq611t3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dumdie, Jennifer Nicole. “Molecular Determinants of Oocyte and Embryo Developmental Competence.” 2019. Web. 18 Apr 2021.

Vancouver:

Dumdie JN. Molecular Determinants of Oocyte and Embryo Developmental Competence. [Internet] [Thesis]. University of California – San Diego; 2019. [cited 2021 Apr 18]. Available from: http://www.escholarship.org/uc/item/0kq611t3.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dumdie JN. Molecular Determinants of Oocyte and Embryo Developmental Competence. [Thesis]. University of California – San Diego; 2019. Available from: http://www.escholarship.org/uc/item/0kq611t3

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

9. Aboussahoud, Wedad. The role of toll-like receptors in assisted conception.

Degree: Thesis (M.D.), 2016, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-tolllike-receptors-in-assisted-conception(230bd2c9-e8bb-490f-b63f-9376667da89c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684801

► Background: In the last two decades the discovery of the Toll-Like Receptor (TLR) family in humans revealed the importance of innate immunity in providing host… (more)

▼ Background: In the last two decades the discovery of the Toll-Like Receptor (TLR) family in humans revealed the importance of innate immunity in providing host defence against invading pathogens. Moreover, TLRs have a vital role in mediating the interactions between the reproductive and immune systems. Similar to TLRs, (NOD)-like receptors (NLRs); retinoic acid-inducible gene-1, RIG-1-like receptors (RLRs) are also important pattern recognition receptors in the female reproductive system. Successful implantation requires effective reciprocal interactions between receptive endometrium and competent embryo. The endometrial innate immunity during implantation has been intensively investigated. However, little is known about the expression of innate immunity during the early stages of human embryo development. Objective: To investigate the expression of innate immunity molecules during the early stages of human embryo development and to determine the functions of TLRs in blastocysts. Material and methods: The expression of TLRs, a panel of downstream signalling molecules, NLRs, RLRs, inflammatory cytokines, chemokines and the hyaluronic acid system were investigated in the following developmental stages: oocyte, 4- cell, blastomeres, 8- cell, blastocyst, inner cell mass and trophectoderm using Affymetrix Human Genome U133 Plus 2.0 arrays. Microarray data were validated by Q-PCR. TLR function in human blastocysts was investigated by treating day five blastocysts with TLR3 or TLR5 specific ligands; Poly (I:C) and flagellin respectively, for 24 hours. The culture media was analysed for elevated cytokine and chemokine levels using cytometric bead array. Results: TLRs, NLRs, RLRs, TLR downstream signalling molecules, cytokines and chemokines involved during implantation event and the hyaluronic system were all found to be positively expressed in the early stages of human embryo development. The expression levels of the above molecules were generally moderate to low (CT 24-34) and varied across the embryonic developmental stages. Stimulation of TLR3 and TLR5 in day 5 blastocysts produced cytokines and chemokines. In addition, there were alterations in gene expression patterns in the Poly (I:C) and flagellin treated blastocysts in comparison to the untreated blastocysts. Conclusion: The varied expression levels of the investigated molecules involved in early embryonic developmental suggests a potential role for these molecules in early pregnancy loss and implantation failure. Specifically, the relationship between the level of TLR expression, function and embryo quality is worth investigating in the future as a potential marker for embryo competence.

Subjects/Keywords: 612; Toll Like Recepors; Human embryos; Embryo implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboussahoud, W. (2016). The role of toll-like receptors in assisted conception. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-tolllike-receptors-in-assisted-conception(230bd2c9-e8bb-490f-b63f-9376667da89c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684801

Chicago Manual of Style (16^th Edition):

Aboussahoud, Wedad. “The role of toll-like receptors in assisted conception.” 2016. Doctoral Dissertation, University of Manchester. Accessed April 18, 2021. https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-tolllike-receptors-in-assisted-conception(230bd2c9-e8bb-490f-b63f-9376667da89c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684801.

MLA Handbook (7^th Edition):

Aboussahoud, Wedad. “The role of toll-like receptors in assisted conception.” 2016. Web. 18 Apr 2021.

Vancouver:

Aboussahoud W. The role of toll-like receptors in assisted conception. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2021 Apr 18]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-tolllike-receptors-in-assisted-conception(230bd2c9-e8bb-490f-b63f-9376667da89c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684801.

Council of Science Editors:

Aboussahoud W. The role of toll-like receptors in assisted conception. [Doctoral Dissertation]. University of Manchester; 2016. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-role-of-tolllike-receptors-in-assisted-conception(230bd2c9-e8bb-490f-b63f-9376667da89c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.684801

University of Illinois – Urbana-Champaign

10. Nallasamy, Shanmugasundaram. Role of Msx homeobox genes in uterus during embryo implantation.

Degree: PhD, 5274, 2012, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/32038

► Implantation of the embryo into the wall of the uterus is a crucial event in mammalian embryogenesis. This complex event involves a series of interactions… (more)

▼ Implantation of the embryo into the wall of the uterus is a crucial event in mammalian embryogenesis. This complex event involves a series of interactions between the developing embryo and the receptive endometrium ultimately leading to successful establishment of pregnancy. The sequential events of implantation include apposition of the blastocyst to the uterine luminal epithelium followed by adhesion to the epithelium and then penetration through the epithelium and basal lamina into the uterine stroma. Uterine sensitivity with respect to implantation has been classified as prereceptive, receptive, and nonreceptive phases. In the mouse, day 1–3 of pregnancy constitutes the prereceptive phase and day 4 is considered receptive. The window of uterine receptivity is transient and lasts for a limited time. On day 5 of pregnancy, the uterus is nonreceptive or refractory. Studies over the past decade have identified a variety of molecules including growth factors, cytokines, transcription factors, and extracellular matrix proteins as potential regulators of this complex process. This dissertation work investigates the critical role of Msx homeobox genes in the uterus during embryo implantation. The mammalian Msx homeobox genes, Msx1 and Msx2, encode transcription factors that control organogenesis and tissue interactions during embryonic development. Uterine specific deletion of Msx1 and Msx2 resulted in female infertility due to a failure in implantation. Further analysis indicated that mice lacking uterine Msx1 and Msx2 (Msx1d/dMsx2d/d) exhibited a failure in uterine receptivity due to enhanced estrogen signaling in the luminal epithelium, failure of microvilli remodeling, sustained epithelial cell polarity and persistent proliferative activity of luminal and glandular epithelium. More recent studies revealed that canonical Wnt/ β-catenin signaling were upregulated in the Msx1Msx2-null uteri, which in turn stimulated the production of a subset of fibroblast growth factors (FGFs) in the stromal cells. The FGFs subsequently activate FGFR-ERK-MAP kinase signaling pathway in the luminal epithelium resulting in sustained epithelial cellular proliferation. These results uncovered a unique signaling network, involving Msx1/2, Wnts, and FGFs, which regulate stromal-epithelial cross talk in the mouse uterus at the time of receptivity. The last chapter addresses the role of Msx homeobox genes during uterine stromal cell decidualization. As the embryo attaches to the uterine wall and invades into the stromal bed, the stromal cells surrounding the implanting blastocyst differentiate into decidual cells in a process known as decidualization. This process is critical for embryo survival, angiogenesis and successful establishment of pregnancy. We previously reported that Bone morphogenetic protein 2 (BMP2) regulates uterine stromal cell differentiation in the mouse and the human. Subsequent studies revealed that the expressions of Msx1 and Msx2 were markedly altered in response to exogenous BMP2. Functional studies performed using… Advisors/Committee Members: Bagchi, Indrani C. (advisor), Bagchi, Indrani C. (Committee Chair), Bagchi, Milan K. (committee member), Flaws, Jodi A. (committee member), Miller, Suzanne E. (committee member).

Subjects/Keywords: Msx homeobox genes; uterus; embryo implantation; uterine receptivity; decidualization; Mouse; Human

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nallasamy, S. (2012). Role of Msx homeobox genes in uterus during embryo implantation. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/32038

Chicago Manual of Style (16^th Edition):

Nallasamy, Shanmugasundaram. “Role of Msx homeobox genes in uterus during embryo implantation.” 2012. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 18, 2021. http://hdl.handle.net/2142/32038.

MLA Handbook (7^th Edition):

Nallasamy, Shanmugasundaram. “Role of Msx homeobox genes in uterus during embryo implantation.” 2012. Web. 18 Apr 2021.

Vancouver:

Nallasamy S. Role of Msx homeobox genes in uterus during embryo implantation. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2142/32038.

Council of Science Editors:

Nallasamy S. Role of Msx homeobox genes in uterus during embryo implantation. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/32038

Universitat de Valencia

11. Balaguer Cuenca, Nuria. Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome .

Degree: 2018, Universitat de Valencia

URL: http://hdl.handle.net/10550/68529

► Introducción En el proceso de implantación embrionaria se produce una relación sincrónica entre el endometrio y el embrión que resulta fundamental para su correcta consecución;… (more)

▼ Introducción En el proceso de implantación embrionaria se produce una relación sincrónica entre el endometrio y el embrión que resulta fundamental para su correcta consecución; dicha sincronía se mantiene durante la gestación gracias a la existencia de una comunicación bidireccional entre la madre y el feto basada en la secreción de señales específicas que permiten regular un desarrollo cooperativo. Efectivamente, se ha observado como el embrión libera moléculas específicas del estado gestacional (ej.: gonadotropina coriónica humana o interferón tau en rumiantes), para prevenir la luteólisis inducida por la prostaglandina F2alpha, posibilitando la secreción continua de progesterona durante la gestación. Del mismo modo, las secreciones uterinas regulan el estado de desarrollo embrionario y promueven la proliferación del trofectodermo, la migración, o la unión al epitelio luminal endometrial. Debido a la relevancia de este proceso de comunicación entre la madre y el embrión, el estudio del microambiente en el que se produce dicha comunicación bidireccional ha suscitado un gran interés recientemente. Entre las publicaciones más destacadas en este campo, se encuentran aquellas que se focalizan en el papel que ejerce el líquido endometrial (LE), tanto en el proceso de implantación como en el desarrollo embrionario posterior. El LE está constituido principalmente por el trasudado plasmático y las secreciones del epitelio luminal y glandular, que aportan toda una batería de compuestos como aminoácidos, iones, carbohidratos, lípidos y proteínas (incluyendo citoquinas, enzimas, hormonas, factores de crecimiento, transportadores, proteasas y factores inmunomoduladores) encargados de suministrar una nutrición temprana al conceptus (embrión junto con sus membranas asociadas) durante el periodo previo a la formación de las estructuras placentarias. La presencia de microARNs (miARNs) en el LE ha suscitado gran interés debido a su función en la regulación de la expresión génica. Los miARNs son moléculas de ARN no codificante de aproximadamente 22-25 nt de longitud capaces de regular hasta cientos de ARN mensajeros (mRNA) diana mediante complementación perfecta o imperfecta con las regiones 3’ no traducidas (UTR) de sus transcritos diana. Normalmente participan en procesos de degradación o inhibición de la traducción, aunque también se han descrito casos de miARNs capaces de inducir expresión proteica (Mehta y Baltimore, 2016). Los miARNs regulan la expresión génica en tejidos fetales y maternos a lo largo de todo el curso gestacional. Inicialmente, se describió la existencia de patrones de expresión diferencial de miARNs en células epiteliales endometriales a lo largo del ciclo menstrual, sugiriendo que su presencia podría estar sujeta a regulación hormonal en el endometrio humano (Kuokannen et al., 2010). Más tarde, se identificó un perfil específico de miARNs en pacientes con fallo recurrente de implantación que inhibían a genes implicados en rutas de señalización cruciales para la implantación embrionaria (ej.: vía de… Advisors/Committee Members: Vilella Mitjana, Felipe (advisor).

Subjects/Keywords: miR-30d; implantation; embryo-maternal crosstalk; exosomes; hnRNPC1

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Balaguer Cuenca, N. (2018). Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome . (Doctoral Dissertation). Universitat de Valencia. Retrieved from http://hdl.handle.net/10550/68529

Chicago Manual of Style (16^th Edition):

Balaguer Cuenca, Nuria. “Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome .” 2018. Doctoral Dissertation, Universitat de Valencia. Accessed April 18, 2021. http://hdl.handle.net/10550/68529.

MLA Handbook (7^th Edition):

Balaguer Cuenca, Nuria. “Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome .” 2018. Web. 18 Apr 2021.

Vancouver:

Balaguer Cuenca N. Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome . [Internet] [Doctoral dissertation]. Universitat de Valencia; 2018. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10550/68529.

Council of Science Editors:

Balaguer Cuenca N. Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome . [Doctoral Dissertation]. Universitat de Valencia; 2018. Available from: http://hdl.handle.net/10550/68529

12. Duval, Fabien. Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy.

Degree: Docteur es, Sciences de la vie et de la santé, 2017, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2017SACLV070

►

L’implantation embryonnaire repose sur une synchronisation spatio-temporelle entre un placenta fonctionnel et un endomètre réceptif. La réceptivité endométriale requiert la différenciation des cellules stromales en… (more)

▼

L’implantation embryonnaire repose sur une synchronisation spatio-temporelle entre un placenta fonctionnel et un endomètre réceptif. La réceptivité endométriale requiert la différenciation des cellules stromales en cellules déciduales sous l’effet des hormones ovariennes (œstrogènes et progestérone). Le placenta est un organe transitoire constitué de deux types cellulaires. Le cytotrophoblaste villeux est responsable des échanges fœtaux maternels et de la fonction endocrine du placenta. Le cytotrophoblaste extravilleux présente des propriétés invasives et assure ainsi l’ancrage du placenta dans l’endomètre maternel. Un dialogue paracrine complexe entre les cellules placentaires et endométriales s’établit au cours des premières étapes de l’implantation.L’adiponectine est une adipokine produite majoritairement par le tissu adipeux. Elle contrôle le métabolisme glucido-lipidique et joue le rôle d’hormone insulino-sensibilisatrice. Dans de nombreux tissus, l’adiponectine exerce des effets anti-prolifératifs, pro-invasifs et pro-différenciants. L’adiponectine et ses récepteurs ADIPOR1 et ADIPOR2 sont présents à l’interface fœto-maternelle. Le placenta et l’endomètre sont donc des tissus cibles de l’adiponectine.Au cours de ce travail, nous nous sommes intéressés aux effets directs de l’adiponectine à l’interface foeto-maternelle au cours du premier trimestre de grossesse.Dans une première partie, nous avons observé que l’adiponectine exerce des effets anti-différenciants et anti-invasifs dans les cellules stromales endométriales.Dans un second temps, nous avons démontré que l’adiponectine favorise la production de glycogène dans les cellules déciduales. Inversement, l’adiponectine semble limiter l’entrée du glycogène dans les cellules placentaires. Ces résultats démontrent que l’adiponectine pourrait contrôler la nutrition histiotrophe du foetus.Dans une dernière partie, nous avons observé que l’adiponectine diminue l’expression des transporteurs de nutriments et exerce une action pro-apoptotique dans le trophoblaste villeux. Ces derniers résultats pourraient permettre de mieux comprendre le rôle de l’adiponectine dans les pathologies placentaires telles que le retard de croissance intra-utérin qui se caractérise par une diminution du poids foetal et une augmentation de l’apoptose des cellules trophoblastiques.L’ensemble de ces résultats montre que l’adiponectine est un acteur clé du dialogue foeto-maternel au cours de la grossesse précoce en contrôlant la maturation d’un endomètre fonctionnel et en régulant les échanges nutritifs transplacentaires.

Embryo implantation requires a spatiotemporal synchronization between a functional placenta and a receptive endometrium. Endometrium receptivity based on the differentiation of stromal cells into decidual cells, under the influence of ovarian hormones (estrogens and progesterone). The placenta is a transient organ composed of two cell types. Villous trophoblast ensures fetal-maternal exchanges and the endocrine functions. Extravillous trophoblast acquire an invasive…

Advisors/Committee Members: Dieudonné, Marie-Noëlle (thesis director).

Subjects/Keywords: Adiponectine; Endomètre humain; Placenta humain; Décidualisation; Implantation embryonnaire; Croissance fœtale; Adiponectin; Human endometrium; Human placenta; Decidualization; Embryo implantation; Fetal growth; 612.6

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Duval, F. (2017). Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2017SACLV070

Chicago Manual of Style (16^th Edition):

Duval, Fabien. “Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy.” 2017. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed April 18, 2021. http://www.theses.fr/2017SACLV070.

MLA Handbook (7^th Edition):

Duval, Fabien. “Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy.” 2017. Web. 18 Apr 2021.

Vancouver:

Duval F. Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2017. [cited 2021 Apr 18]. Available from: http://www.theses.fr/2017SACLV070.

Council of Science Editors:

Duval F. Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse : Adiponectin roles at the human fetal-maternal interface in first-trimester of pregnancy. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2017. Available from: http://www.theses.fr/2017SACLV070

University of Saskatchewan

13. Alisha, Alisha 1984-. Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques.

Degree: 2015, University of Saskatchewan

URL: http://hdl.handle.net/10388/12629

► The overall objective of this thesis was to analyze developmental and gene expression changes in cryopreserved in vitro produced (IVP) bovine embryos using two techniques;… (more)

▼ The overall objective of this thesis was to analyze developmental and gene expression changes in cryopreserved in vitro produced (IVP) bovine embryos using two techniques; vitrification and slow freezing. Specifically, the first objective was to study and compare the blastocyst development of IVP bovine day 6 morulae [day 0 = in vitro fertilization (IVF)] after cryopreservation using vitrification and slow freezing (end point = development to expanded blastocyst stage on day 7-8). The blastocyst development rate for the vitrification group (52±4.6%) was higher (p<0.001) than that of the slow freezing group (35±4.2%). Blastocyst development rate was the highest in unfrozen control group (78 ± 3.6%). Re-expansion of vitrified morulae upon warming and correlation with subsequent blastocyst conversion rate was studied. No significant correlation was found between the vitrified morula re-expansion upon warming and blastocyst rate (Pearson’s correlation = -0.048; p>0.05). Data obtained from above study of cryopreserved embryos was also utilized for comparing the effect of season (cleavage rate and morula rate) and season x cryopreservation interaction on blastocyst conversion rate. Fall season had lower cleavage (67±1.6%; day 2) and morula (22±1.4%; day 6) rates than other seasons (74±1.1% and 30±1.2%, respectively; p<0.05). Spring, summer and winter seasons did not differ (p>0.05). Blastocyst conversion rate differed (p<0.05) between summer (63±4.5%) and spring (85±3.3%) seasons in unfrozen groups, between summer (20±4.1%) and all other seasons (43±8.3%) in slow freezing groups and did not differ among the seasons in the vitrification group (p=0.19). Another aim of the study was to determine the effect of different cryoprotectants (glycerol and ethylene glycol) on the blastocyst conversion rates from slow frozen morulae. The blastocyst conversion rate had a tendency (p=0.065) to be higher in the ethylene glycol group (31±5.3%) than the glycerol group (18±4.3%). A final set of experiments was designed to compare the global gene expression analysis of vitrified and slow frozen IVP embryos to control IVP embryos. Day 6 IVP bovine morulae were randomly distributed across three groups: unfrozen control, vitrified and slow frozen. These embryos were allowed to grow to expanded blastocyst stage in culture for 24-48 h without treatment (control) or after warming (vitrification and slow freezing groups). Four successful replicates were conducted for each group on separate dates (6-7 embryos per group per replicate). Total RNA was extracted, RNA quality was tested using bio-analyzer and samples were hybridized on microarray slides. Images acquired from microarray slides were analyzed using ArrayPro™, ELMA, FlexArray and Ingenuity pathway analysis softwares. The vitrified group had 64 differentially regulated (up and down regulated) genes as compared with control group, while a total of 162 genes were differentially expressed in slow frozen group as compared with control group. Upon in silico analysis differential gene expression… Advisors/Committee Members: Anzar, Muhammad, Singh, Jaswant, Robert, Claude.

Subjects/Keywords: Bovine embryo; Vitrification; Slow freezing; Lipid metabolism; Microarray; Real time PCR; Cryopreservation; Morula; IVF; implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alisha, A. 1. (2015). Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alisha, Alisha 1984-. “Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques.” 2015. Thesis, University of Saskatchewan. Accessed April 18, 2021. http://hdl.handle.net/10388/12629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alisha, Alisha 1984-. “Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques.” 2015. Web. 18 Apr 2021.

Vancouver:

Alisha A1. Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques. [Internet] [Thesis]. University of Saskatchewan; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10388/12629.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alisha A1. Global gene expression analysis of in vitro produced cryopreserved bovine embryos using vitrification and slow freezing techniques. [Thesis]. University of Saskatchewan; 2015. Available from: http://hdl.handle.net/10388/12629

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

14. Kyprianou, Christos. Morphogenesis of the early post-implantation mouse embryo.

Degree: PhD, 2019, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/290301

► The morphogenetic events that give rise to the early post-implantation mouse embryo (egg cylinder) have not been thoroughly studied and our knowledge is restricted to… (more)

▼ The morphogenetic events that give rise to the early post-implantation mouse embryo (egg cylinder) have not been thoroughly studied and our knowledge is restricted to “snap-shot” descriptions of embryos recovered at different stages of implantation from the mother. A central feature of the egg cylinder is the pro-amniotic cavity, which spans the embryo and participates in formation of the extraembryonic membranes. The major aims of my PhD studies have been to reveal how this cavity is formed (Aim 1) and then how the egg cylinder grows (Aim 2). In order to address how the pro-amniotic cavity forms (Aim 1), I first characterised in detail development of the architecture of the extra-embryonic ectoderm (ExE), which has to be remodelled to permit cavity formation. My findings indicate that the ExE comprises cells in direct contact with a basement membrane and cells that lie deeper in the tissue. The ExE originates in the polar trophectoderm, a monolayer covering the epiblast of the blastocyst, which expands and undergoes invagination to form a slit-like cavity. By carrying out analyses of fixed specimens and live imaging of cultured embryos, I have found that the epiblast and ExE cavity extend towards each other through the formation and resolution of multiple rosette structures. This leads to the fusion of the ExE and epiblast cavities to form the unified pro-amniotic cavity. I show that this process is dependent on signalling cues stemming from the underlying basement membrane that activate the b1-integrin signalling pathway to regulate cell polarity, ExE tissue architecture and rosette formation. In addition to the basement membrane’s role in b1-integrin signalling, it also has physical functions that I characterise in the second part of my study (Aim 2). High resolution imaging revealed that the basement membrane underlying the epiblast is highly perforated during the implantation stages. These perforations are initially evenly distributed and then accumulate asymmetrically at the future posterior part of the embryo, just prior to gastrulation. Finally, I demonstrate that remodelling of the basement membrane requires the expression of matrix metalloproteinases (MMPs) in the epiblast under the control of Nodal. The anterior visceral endoderm inhibits Nodal signalling and hence MMP inhibition in the anterior. I demonstrate that activity of the MMPs and perforations in the basement membrane are essential for embryo growth. The domain of posterior basement membrane perforations persists beyond gastrulation suggesting a potential role for these perforations in primitive streak formation and extension. Together, my studies bring new important insights into the understanding of early mouse embryo morphogenesis.

Subjects/Keywords: Developmental Biology; Rosettes; extraembryonic ectoderm; post-implantation; murine; mouse; embryo; basement membrane; perforations

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kyprianou, C. (2019). Morphogenesis of the early post-implantation mouse embryo. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/290301

Chicago Manual of Style (16^th Edition):

Kyprianou, Christos. “Morphogenesis of the early post-implantation mouse embryo.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021. https://www.repository.cam.ac.uk/handle/1810/290301.

MLA Handbook (7^th Edition):

Kyprianou, Christos. “Morphogenesis of the early post-implantation mouse embryo.” 2019. Web. 18 Apr 2021.

Vancouver:

Kyprianou C. Morphogenesis of the early post-implantation mouse embryo. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18]. Available from: https://www.repository.cam.ac.uk/handle/1810/290301.

Council of Science Editors:

Kyprianou C. Morphogenesis of the early post-implantation mouse embryo. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/290301

University of Cambridge

15. Kyprianou, Christos. Morphogenesis of the early post-implantation mouse embryo.

Degree: PhD, 2019, University of Cambridge

URL: https://doi.org/10.17863/CAM.37530 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767902

► The morphogenetic events that give rise to the early post-implantation mouse embryo (egg cylinder) have not been thoroughly studied and our knowledge is restricted to… (more)

▼ The morphogenetic events that give rise to the early post-implantation mouse embryo (egg cylinder) have not been thoroughly studied and our knowledge is restricted to "snap-shot" descriptions of embryos recovered at different stages of implantation from the mother. A central feature of the egg cylinder is the pro-amniotic cavity, which spans the embryo and participates in formation of the extraembryonic membranes. The major aims of my PhD studies have been to reveal how this cavity is formed (Aim 1) and then how the egg cylinder grows (Aim 2). In order to address how the pro-amniotic cavity forms (Aim 1), I first characterised in detail development of the architecture of the extra-embryonic ectoderm (ExE), which has to be remodelled to permit cavity formation. My findings indicate that the ExE comprises cells in direct contact with a basement membrane and cells that lie deeper in the tissue. The ExE originates in the polar trophectoderm, a monolayer covering the epiblast of the blastocyst, which expands and undergoes invagination to form a slit-like cavity. By carrying out analyses of fixed specimens and live imaging of cultured embryos, I have found that the epiblast and ExE cavity extend towards each other through the formation and resolution of multiple rosette structures. This leads to the fusion of the ExE and epiblast cavities to form the unified pro-amniotic cavity. I show that this process is dependent on signalling cues stemming from the underlying basement membrane that activate the b1-integrin signalling pathway to regulate cell polarity, ExE tissue architecture and rosette formation. In addition to the basement membrane's role in b1-integrin signalling, it also has physical functions that I characterise in the second part of my study (Aim 2). High resolution imaging revealed that the basement membrane underlying the epiblast is highly perforated during the implantation stages. These perforations are initially evenly distributed and then accumulate asymmetrically at the future posterior part of the embryo, just prior to gastrulation. Finally, I demonstrate that remodelling of the basement membrane requires the expression of matrix metalloproteinases (MMPs) in the epiblast under the control of Nodal. The anterior visceral endoderm inhibits Nodal signalling and hence MMP inhibition in the anterior. I demonstrate that activity of the MMPs and perforations in the basement membrane are essential for embryo growth. The domain of posterior basement membrane perforations persists beyond gastrulation suggesting a potential role for these perforations in primitive streak formation and extension. Together, my studies bring new important insights into the understanding of early mouse embryo morphogenesis.

Subjects/Keywords: 571.8; Developmental Biology; Rosettes; extraembryonic ectoderm; post-implantation; murine; mouse; embryo; basement membrane; perforations

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kyprianou, C. (2019). Morphogenesis of the early post-implantation mouse embryo. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.37530 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767902

Chicago Manual of Style (16^th Edition):

Kyprianou, Christos. “Morphogenesis of the early post-implantation mouse embryo.” 2019. Doctoral Dissertation, University of Cambridge. Accessed April 18, 2021. https://doi.org/10.17863/CAM.37530 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767902.

MLA Handbook (7^th Edition):

Kyprianou, Christos. “Morphogenesis of the early post-implantation mouse embryo.” 2019. Web. 18 Apr 2021.

Vancouver:

Kyprianou C. Morphogenesis of the early post-implantation mouse embryo. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Apr 18]. Available from: https://doi.org/10.17863/CAM.37530 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767902.

Council of Science Editors:

Kyprianou C. Morphogenesis of the early post-implantation mouse embryo. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://doi.org/10.17863/CAM.37530 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.767902

University of Georgia

16. Zhao, Fei. Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development.

Degree: 2015, University of Georgia

URL: http://hdl.handle.net/10724/31357

► Reproduction is essential for the continued existence of species. Both environmental and genetic factors can affect reproduction. This dissertation has two main focuses: I) effects… (more)

▼ Reproduction is essential for the continued existence of species. Both environmental and genetic factors can affect reproduction. This dissertation has two main focuses: I) effects and mechanisms of estrogenic endocrine disruptors, zearalenone (ZEA) and diethylstilbestrol (DES), on puberty and female reproduction; II) effects of a point mutation in lipoma HMGIC fusion partner like-2 (Lhfpl2) gene on distal reproductive tract development in mice. Results from the 1st part of the research can be summarized as follows: A. Postweaning exposure to dietary ZEA advanced the timing of pubertal onset indicated by vaginal opening and disrupted embryo implantation in a dose-dependent way. Pre-mating or post-mating exposure to 40 ppm ZEA in diet affected fertilization or delayed embryo transport and embryo development, respectively, to impair embryo implantation (Chapter 2). B. Multigenerational exposure to 20 ppm dietary ZEA cumulatively impaired fertility, which could be partially alleviated upon exposure cessation (Chapter 3). C. Exposure timing is critical for endocrine disruption of puberty and early pregnancy. Peripubertal exposure to 50 ppb DES significantly advanced the timing of vaginal opening. Peripubertal exposure to 50 ppb DES reduced the numbers of corpora lutea whereas post-mating exposure to 50 ppb DES affected postovulation events, both led to impaired embryo implantation (Chapter 4). The initial goal in part II of the dissertation was to investigate the mechanisms of vaginal opening using a Lhfpl2 mutant mouse model that had vaginal imperforation (Chapter 5). However, extensive studies showed that the vaginal imperforation was due to defective embryonic development of the distal vagina, resulting in female infertility. Interestingly, similar pathology was observed in Lhfpl2 mutant males that had abnormal structure of the distal reproductive tract, causing infertility in ~70% of males. In situ hybridization and immunohistochemistry localized Lhfpl2/LHFPL2 in the epithelium of female and male reproductive tracts. In vitro overexpression studies showed that wild-type and mutant LHFPL2 were both localized in the endoplasmic reticulum but only the wild-type LHFPL2 was expressed in the filopodia. This dissertation provides novel knowledge about mechanisms of estrogenic endocrine disruption of female puberty and reproduction, as well as a genetic basis of distal reproductive tract development.

Subjects/Keywords: Diethylstilbestrol; embryo development; embryo implantation; exposure timing; embryo transport; endocrine disruptors; fertilization; lipoma HMGIC fusion partner like-2; ovulation; reproductive tract development; vaginal opening; zearalenone

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhao, F. (2015). Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/31357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhao, Fei. “Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development.” 2015. Thesis, University of Georgia. Accessed April 18, 2021. http://hdl.handle.net/10724/31357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhao, Fei. “Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development.” 2015. Web. 18 Apr 2021.

Vancouver:

Zhao F. Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development. [Internet] [Thesis]. University of Georgia; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10724/31357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhao F. Mechanisms of estrogenic endocrine disruption of female puberty and reproduction and functions of LHFPL2 on distal reproductive tract development. [Thesis]. University of Georgia; 2015. Available from: http://hdl.handle.net/10724/31357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Gibson, C.M.E. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.

Degree: 2019, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

► The pre-implantation period is a critical time for the establishment and maintenance of pregnancy and, in the mare as in other species, is characterized by… (more)

▼ The pre-implantation period is a critical time for the establishment and maintenance of pregnancy and, in the mare as in other species, is characterized by a high incidence of embryonic loss. The equine conceptus develops for an usually long period within the uterine cavity (40-42 days) before implantation begins and a stable attachment is formed. Moreover, at the time of implantation, the equine embryo has already undergone a number of critical developmental events such as gastrulation, neurulation and initial organogenesis to reach the fetal stage. During its prolonged pre-implantation period, the equine conceptus is entirely dependent on the uterine luminal environment and, in particular, on the uterine secretions or ‘histotroph’ for nutrient provision. Since the luminal epithelium and endometrial glands synthesize, transport and/or secrete the histotroph into the uterine lumen, it is important to understand the mechanisms involved in regulating endometrial secretion of the molecules required for conceptus survival and development. The main focus of this thesis was to improve our understanding of the role of the uterine environment in supporting conceptus development and, in particular, to dissect the importance of maternal progesterone and conceptus-secreted factors in preparing the uterus for its role during pre-implantation development in the horse. To better understand the impact of the uterine environment on conceptus development, and to differentiate between the effects of progesterone and/or the embryo on endometrial gene expression we employed an equine asynchronous embryo transfer model. In this thesis, we show that the horse conceptus is sensitive to the uterine environment to which it is exposed and, in the case of uterine asynchrony, is able to adapt its development accordingly, in order to ensure subsequent roughly synchronous development. Reflecting this adaptability, we found that the endometrial transcriptome is subtly different in a uterus in the presence of a more advanced embryo (i.e. very few DEGs). By contrast, the conceptus is markedly affected (retarded) by a less advanced uterus; this is mainly a result of a shorter duration of endometrial exposure to progesterone, but presumably compounded by the influence of the conceptus itself as it succumbs to developmental retardation. The different gene expression profile of a uterus exposed to a shorter period of progesterone priming will result in a different histotrophic composition, which is presumably the primary signal/factor resulting in developmental retardation of the conceptus. Interestingly, the equine conceptus appears to have an unusual ability to not only sense the changes in the uterine environment but also to delay its development and wait for the endometrium to catch-up, thereby allowing the conceptus to survive. In this respect, it is possible that specific pathways (for example the kinin-kallikrein system) assist or enable the conceptus to detect the stage of the endometrium and regulate its development accordingly. Finally,… Advisors/Committee Members: Stout, Tom, de Ruijter - Villani, Marta.

Subjects/Keywords: conceptus; endometrium; pre-implantation period; asynchronous embryo transfer; horse; embryo development

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gibson, C. M. E. (2019). Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

Chicago Manual of Style (16^th Edition):

Gibson, C M E. “Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.” 2019. Doctoral Dissertation, University Utrecht. Accessed April 18, 2021. https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443.

MLA Handbook (7^th Edition):

Gibson, C M E. “Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?.” 2019. Web. 18 Apr 2021.

Vancouver:

Gibson CME. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. [Internet] [Doctoral dissertation]. University Utrecht; 2019. [cited 2021 Apr 18]. Available from: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443.

Council of Science Editors:

Gibson CME. Pre-implantation conceptus-maternal communication in the horse : What can we learn from asynchronous embryo transfer?. [Doctoral Dissertation]. University Utrecht; 2019. Available from: https://dspace.library.uu.nl/handle/1874/376443 ; URN:NBN:NL:UI:10-1874-376443 ; 1874/376443 ; urn:isbn:9789463801959 ; URN:NBN:NL:UI:10-1874-376443 ; https://dspace.library.uu.nl/handle/1874/376443

18. Alessanda Fernandes Louzada Hoegemann Ramos. Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar.

Degree: 2007, Universidade Federal de Juiz de Fora

URL: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=65

► O tacrolimus é um antibiótico macrolídeo com ação imunossupressora, atuando como inibidor de calcineurina, que vem sendo utilizado como droga base na maioria dos transplantes… (more)

▼ O tacrolimus é um antibiótico macrolídeo com ação imunossupressora, atuando como inibidor de calcineurina, que vem sendo utilizado como droga base na maioria dos transplantes para evitar rejeição. Com o êxito dos transplantes, decorrente principalmente do uso de imunossupressores, houve uma melhora na qualidade de vida das pacientes transplantadas. Esta melhora levou ao aumento do número de gestações, mesmo sob o uso contínuo de imunossupressores. Sabe-se que os imunossupressores atravessam a placenta podendo levar a alterações no desenvolvimento do embrião. Segundo a literatura, foram observados desvios da normalidade, como perda de peso, em recém nascidos de mães que utilizavam o tacrolimus. Entretanto, pouco se sabe sobre a sua atuação no período de préimplantação do blastocisto, sendo o objetivo deste trabalho verificar se o tacrolimus interfere no desenvolvimento do embrião de rata, durante seu trânsito tubário e na fase de implantação do blastocisto. Ratas Wistar prenhes foram distribuídas aleatoriamente nos grupos controles C1 e C2 e tratados T1, T2, T3 e T4, T5 e T6. Controle 1 e Tratados 1, 2 e 3 receberam água destilada e 1, 2 e 4 mg/kg/dia de Tacrolimus respectivamente por via intragástrica do primeiro ao quinto dia de prenhez (período de trânsito tubário) e os grupos Controle 2 e Tratados 4, 5 e 6 receberam água destilada e 1, 2 e 4 mg/kg/dia de tacrolimus , respectivamente, por via intragástrica, do quinto ao sétimo dia (período de implantação). O acompanhamento de sinais clínicos maternos possibilitou a análise de efeitos tóxicos sobre a mãe durante o período de gestação. No 150 dia, foi coletado sangue para avaliação de parâmetros bioquímicos e hematológicos, em seguida os animais foram eutanasiados. Ovários, fígado e rim foram pesados e os corpos lúteos contados. Foram identificados fetos vivos, reabsorções e fetos mortos. Fetos e placentas foram pesados. Não foram encontrados indícios clínicos de toxicidade materna. No período de trânsito tubário não foram observadas alterações significativas no peso corporal, consumo de ração e parâmetros hematológicos das mães. Na análise bioquímica, o grupo da dose de 4 mg/kg/dia apresentou aumento na concentração de colesterol, de TGO e de uréia. No grupo com dose de 2mg/kg/dia ocorreu redução de creatinina e no grupo T1 houve uma redução de TGO. No período de implantação do blastocisto, não foram observadas variações no peso corporal entre os grupos analisados. Foi observada uma diminuição no consumo de ração, durante o período de tratamento, que foi restabelecido com o término deste, apresentando um aumento gradativo do consumo até o final do experimento. O tacrolimus na concentração de 4mg/Kg/dia apresentou consumo médio superior aos demais grupos. Os parâmetros hematológicos não apresentaram alterações significativas. Os parâmetros bioquímicos não apresentaram alterações relevantes entre os grupos experimentais, exceto os referentes à TGO, que apresentou uma diminuição no grupo T6. Nos estudos realizados no período de trânsito tubário e no de implantação,… Advisors/Committee Members: Martha de Oliveira Guerra, Hélady Sanders Pinheiro, Tania Toledo de Oliveira, Vera Maria Peters.

Subjects/Keywords: rat; immunosuppressor; pregnancy; implantation; toxicity; CIENCIAS DA SAUDE; tacrolimus; embriotoxicidade; rato; imunossupressor; gravidez; implantação; tacrolimus; embryo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramos, A. F. L. H. (2007). Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar. (Thesis). Universidade Federal de Juiz de Fora. Retrieved from http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=65

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ramos, Alessanda Fernandes Louzada Hoegemann. “Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar.” 2007. Thesis, Universidade Federal de Juiz de Fora. Accessed April 18, 2021. http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=65.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ramos, Alessanda Fernandes Louzada Hoegemann. “Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar.” 2007. Web. 18 Apr 2021.

Vancouver:

Ramos AFLH. Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar. [Internet] [Thesis]. Universidade Federal de Juiz de Fora; 2007. [cited 2021 Apr 18]. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=65.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ramos AFLH. Avaliação do potencial embriotóxico do Tacrolimus (Fk506) administrado a ratas Wistar. [Thesis]. Universidade Federal de Juiz de Fora; 2007. Available from: http://www.bdtd.ufjf.br/tde_busca/arquivo.php?codArquivo=65

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

19. Blois, Sandra M. Regulation of the immunotolerance during the early stages of pregnancy.

Degree: 2010, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/11677

► Successful mammalian pregnacy relies on the development of maternal immune tolerance towards the fetal semiallograft, which has been referred to as immunity's pregnant pause. Fetal… (more)

▼ Successful mammalian pregnacy relies on the development of maternal immune tolerance towards the fetal semiallograft, which has been referred to as immunity's pregnant pause. Fetal tolerance results from a complex interplay involving steroid hormones, cytokines and other soluble factors that modulate leukocyte functions at the maternal fetal interface. For instance, low levels of progesterone and predominance of Th1 type cytokines have often been associated with increased abortion rates, which can also be boosted by psychoemotional stress. In mice, the abortogenic effects of stress can be counteracted by treatment with dydrogesterone (a progesterone derivative highly selective for the progesterone receptor), decreasing the frequency of abortogenic cytokines such as TNF-α, IL-12 and IFN-γ. Experiments in vivo have also shown that stress-triggered fetal rejection can be prevented by interfering with the molecular cross talk between ICAM-1, expressed on antigen presenting cells and its ligand, LFA-1, restoring immune acceptance mechanisms in challenged pregnancies. Intercellular adhesion events involving the ICAM-1/LFA-1 pathway mediate the recruitment of proinflammatory cells to the implantation site, promote dendritic cell (DC) maturation in the decidua, and subsequently induce additional local Th1 polarization via mature DCs. More recently, progesterone has been shown to act in synergy with Galectin (Gal)-1 (an immunoregulatory glycan-binding protein) to promote fetal tolerance. Consistently with a marked decrease in Gal-1 expression during failing pregnancies, treatment with recombinant Gal-1 restored fetal immune acceptance through multiple mechanisms, including the induction of tolerogenic DC, which in turn promoted the expansion of interleukin-10 (IL-10)-secreting regulatory T cells in vivo. Antigen presenting cells, mediating the first encounter with non-self antigens, are likely to be the core of immunoregulatory mechanisms acting on mucosal surfaces. Particularly in the decidua, DC have emerged as an important regulatory population given their ability to interact with other cellular components of the uterine milieu (i.e., natural killer cells) and modulate the nature of immune responses in stimulatory or tolerogenic fashion. Studies in vitro have shown that such DC-NK cell cross talk can result in the promotion of a tolerogenic microenvironment characterized by downregulation of the expression of activation markers on uterine NK cells and DC and dominance of pregnancy-protective Th2 cytokines. NK and DC interactions were also shown to influence uterine cell proliferation, providing the first clues into a role played by these cells during the process of endometrial decidualization. The important role played by DC during early pregnancy was further highlighted by studies in vivo showing that transient depletion of CD11c+ cells (i.e., through administration of diphtheria toxin) impairs the implantation process in mice, resulting in a reduced breeding efficiency. The analysis of DC- depleted implantation sites… Advisors/Committee Members: [email protected] (contact), w (gender), Herr Prof. Dr. J. Dietl , Würzburg (firstReferee), Herr Prof. Dr. C. J. Thaler, München (furtherReferee).

Subjects/Keywords: Immunology; pregnancy; dendritic cells; galektin-1; embryo implantation; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Blois, S. M. (2010). Regulation of the immunotolerance during the early stages of pregnancy. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/11677

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Blois, Sandra M. “Regulation of the immunotolerance during the early stages of pregnancy.” 2010. Thesis, Freie Universität Berlin. Accessed April 18, 2021. https://refubium.fu-berlin.de/handle/fub188/11677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Blois, Sandra M. “Regulation of the immunotolerance during the early stages of pregnancy.” 2010. Web. 18 Apr 2021.

Vancouver:

Blois SM. Regulation of the immunotolerance during the early stages of pregnancy. [Internet] [Thesis]. Freie Universität Berlin; 2010. [cited 2021 Apr 18]. Available from: https://refubium.fu-berlin.de/handle/fub188/11677.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Blois SM. Regulation of the immunotolerance during the early stages of pregnancy. [Thesis]. Freie Universität Berlin; 2010. Available from: https://refubium.fu-berlin.de/handle/fub188/11677

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Filis, Panayiotis. Roles of PLCβ1 in female reproduction.

Degree: PhD, 2011, University of Edinburgh

URL: http://hdl.handle.net/1842/5701

► In mammals, development of a new organism requires fertilisation of the female egg by sperm. The resulting zygote develops into the blastocyst stage as it… (more)

▼ In mammals, development of a new organism requires fertilisation of the female egg by sperm. The resulting zygote develops into the blastocyst stage as it travels towards the uterus. Within the uterus, the blastocyst invades the maternal tissues and establishes access to the maternal blood supply. This process is called implantation and is absolutely essential for the further development of the conceptus and establishment of pregnancy. Successful implantation requires a proper preparation of the uterus and the embryo as well as a molecular dialogue between the embryo and the uterine tissues. Female mice that have a disruption in the Plcβ1 gene are infertile. In the course of this Thesis it became apparent that the main cause of their infertility is their inability to implant their embryos. PLCβ1 protein is a mediator of G-protein coupled receptor (GPCR) signalling and it is involved in the production of second messengers essential for downstream transmission of signals. A host of reproductive functions are under the control of GPCR signalling. In this PhD Thesis the infertile phenotype of Plcβ1 knockout (KO) female mice was investigated to identify the reproductive processes affected by the lack of a functional PLCβ1 protein. A combination of histological, molecular biology and in vivo techniques were utilised to show that at the time of implantation, embryos fail to attach to the uterine epithelium of KO uteri. In addition, it was demonstrated that estrogen signalling and components of the endocannabinoid metabolism, both key processes for successful implantation are severely altered in KO uteri. These observations show that KO uteri fail to prepare for implantation. In addition, the KO reproductive tract exerts a detrimental effect on pre- and peri- implantation embryo development. Currently, failure of implantation is thought to be one of the major causes of infertility in women and up to this date there are no successful treatments. The results of this project expand our current knowledge on the physiology of implantation and provide cues for the development of diagnostic markers and treatments for the women who are unable to conceive.

Subjects/Keywords: 612.6; uterus; implantation; reproduction; females; embryo

…effect on pre- and peri- implantation embryo development. Currently, failure of implantation is… …implantation at all times, while the embryo is required to reach at a certain development stage to… …embryo implantation: clues from mouse models. Nat Rev Genet. 7:185-99) the embryo… …layer that interacts with the embryo, is involved in its implantation and further development… …embryo implantation: clues from mouse models. Nat Rev Genet. 7:185-99 and endometrial dynamics…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Filis, P. (2011). Roles of PLCβ1 in female reproduction. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5701

Chicago Manual of Style (16^th Edition):

Filis, Panayiotis. “Roles of PLCβ1 in female reproduction.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed April 18, 2021. http://hdl.handle.net/1842/5701.

MLA Handbook (7^th Edition):

Filis, Panayiotis. “Roles of PLCβ1 in female reproduction.” 2011. Web. 18 Apr 2021.

Vancouver:

Filis P. Roles of PLCβ1 in female reproduction. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1842/5701.

Council of Science Editors:

Filis P. Roles of PLCβ1 in female reproduction. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5701

21. Dani Ejzenberg. Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis.

Degree: 2012, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5139/tde-27112012-101043/

►

OBJETIVO: avaliar a histomorfometria do endométrio na fase lútea de mulheres férteis e inférteis. MÉTODOS: foram triadas 40 pacientes, 30 inférteis e 10 férteis, em… (more)

▼

OBJETIVO: avaliar a histomorfometria do endométrio na fase lútea de mulheres férteis e inférteis. MÉTODOS: foram triadas 40 pacientes, 30 inférteis e 10 férteis, em seguimento na Clínica Ginecológica do HC-FMUSP, que concordaram em participar deste estudo. Foi realizada avaliação ultrassonográfica seriada a partir da menstruação, para determinação da ovulação. Na fase lútea as pacientes eram submetidas à histeroscopia. Foram excluídas 14 pacientes sendo 12 por falta durante a avaliação ultrassonográfica e 2 pela presença de pólipos. A casuística foi composta por 6 controles férteis, que foram comparadas a 20 casos inférteis (endometriose-8, causa tubo-peritoneal-5, causa masculina-5, sem causa aparente-2). Na histeroscopia foram coletadas duas biópsias dirigidas (sistema de Bettocchi) da parede posterior (terço distal), e da parede anterior (terço médio), e uma biópsia aspirativa com Pipelle. Foram avaliados parâmetros histomorfométricos endometriais. RESULTADOS: as duas formas de biópsia foram apropriadas para análise endometrial; a dirigida coletou menor área tecidual, porém sem sangue. Nenhum paciente fértil apresentou heterogeneidade endometrial (atraso de fase em algum sítio); isto ocorreu em 7 (35%) das inférteis (p=0,11). Foi diagnosticada endometrite em 2 (10%) casos. CONCLUSÃO: não foram observadas diferenças histomorfométricas entre o endométrio de mulheres férteis e inférteis na fase lútea. Parte das pacientes inférteis mostrou heterogeneidade endometrial e endometrite. A biópsia dirigida, assim como a aspirativa, foi adequada ao estudo endometrial na fase lútea

Objective: to evaluate the endometrial histomorphometry of fertile and infertile women during their luteal period. Methods: 40 female patients- 30 infertile and 10 fertile- who were being followed-up at the Gynecological Clinic at the Hospital das Clinicas (HC),-Faculdade de Medicina da Universidade de Sao Paulo (FMUSP), agreed to participate in the study. Serial ultra sonograms, starting from their menstrual period, were performed to identify their ovulation. In the luteal phase the patients underwent hysteroscopy. From the initial sample, 14 patients were excluded from the study, 12 of whom for being absent during scheduled ultra sonograms and 2 for presenting polyps. The final sample thus consisted of 6 fertile females (control subjects) who were compared to 20 patients with infertility, categorized as follows: 8 due to endometriosis, 5 due to peritoneal tube conditions, 5 due to male infertility, and 2 with no apparent cause. During the hysteroscopy 2 directed biopsies (Bettocchis System) of the posterior wall (distal third section) and of the anterior wall (medial third) were performed, as well as Pipelle sampling. Endometrial histomorpholometric parameters were evaluated. Results: the two forms of endometrial sampling performed were appropriate for the endometrial analysis. The directed biopsy collected tissue from a smaller area, but it had no blood. None of the fertile patients presented endometrial heterogeneity, i.e., phase…

Advisors/Committee Members: Paulo Cesar Serafini, Gustavo Arantes Rosa Maciel, Paula Andrea de Albuquerque Salles Navarro, Sérgio Edgar Camões Conti Ribeiro, Manuel de Jesus Simoes.

Subjects/Keywords: Biópsia/métodos; Endométrio/anatomia & histologia; Histeroscopia; Implantação do embrião; Infertilidade; Biopsy/methods; Embryo implantation; Endometrium/anatomy & histology; Hysteroscopy; Infertility

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ejzenberg, D. (2012). Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5139/tde-27112012-101043/

Chicago Manual of Style (16^th Edition):

Ejzenberg, Dani. “Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis.” 2012. Doctoral Dissertation, University of São Paulo. Accessed April 18, 2021. http://www.teses.usp.br/teses/disponiveis/5/5139/tde-27112012-101043/.

MLA Handbook (7^th Edition):

Ejzenberg, Dani. “Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis.” 2012. Web. 18 Apr 2021.

Vancouver:

Ejzenberg D. Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis. [Internet] [Doctoral dissertation]. University of São Paulo; 2012. [cited 2021 Apr 18]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5139/tde-27112012-101043/.

Council of Science Editors:

Ejzenberg D. Avaliação histomorfométrica do endométrio na fase lútea de mulheres férteis e inférteis. [Doctoral Dissertation]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/5/5139/tde-27112012-101043/

22. Rodrigo Barbano Weingrill. Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos.

Degree: 2015, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-07122015-195142/

►

Neste estudo foi analisada a expressão gênica e protéica, uterina e embrionária da quimiocina Ccl25 e de seu receptor Ccr9 nas fases iniciais da implantação… (more)

Subjects/Keywords: Blastocisto; Ccl25; Ccr9; Cone ectoplacentário; Implantação embrionária; Quimiocinas; Blastocyst; Ccl25; Ccr9; Chemokines; Ectoplacental cone; Embryo implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weingrill, R. B. (2015). Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42134/tde-07122015-195142/

Chicago Manual of Style (16^th Edition):

Weingrill, Rodrigo Barbano. “Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos.” 2015. Doctoral Dissertation, University of São Paulo. Accessed April 18, 2021. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-07122015-195142/.

MLA Handbook (7^th Edition):

Weingrill, Rodrigo Barbano. “Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos.” 2015. Web. 18 Apr 2021.

Vancouver:

Weingrill RB. Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos. [Internet] [Doctoral dissertation]. University of São Paulo; 2015. [cited 2021 Apr 18]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-07122015-195142/.

Council of Science Editors:

Weingrill RB. Expressão da quimiocina Ccl25 e seu receptor Ccr9 no processo de implantação embrionária em camundongos. [Doctoral Dissertation]. University of São Paulo; 2015. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-07122015-195142/

23. Camozzato, Giovani Casanova. Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas.

Degree: 2018, Brazil

URL: http://hdl.handle.net/10183/183449

►

A gestação inicial da égua é um período fascinante que abrange numerosas e intensas mudanças em seu desenvolvimento, muitas das quais são únicas para a… (more)

▼

A gestação inicial da égua é um período fascinante que abrange numerosas e intensas mudanças em seu desenvolvimento, muitas das quais são únicas para a espécie equina. Esse desenvolvimento depende da manutenção da função lútea, do estabelecimento de um ambiente uterino e de uma interação precisa e orquestrada entre o concepto e o ambiente uterino. O objetivo deste estudo foi verificar as alterações histológicas do endométrio e a produção histotrófica em éguas cíclicas e prenhes nos dias 7, 10 e 13 pós-ovulação. No primeiro ciclo, biópsias endometriais de 30 éguas foram coletadas no dia 7 (n = 10), 10 (n = 10) e 13 (n = 10) constituindo o grupo éguas cíclicas. No segundo ciclo, as mesmas éguas foram cobertas por um garanhão fértil, acompanhadas diariamente até detectar a ovulação, considerada o dia 0. Foram coletadas biópsias endometriais nos dias7 (n 10), 10 (n 10) e 13 (n 10). Imediatamente após a coleta, o útero foi lavado e as éguas em que foi obtido embrião, foram inseridas no grupo de éguas prenhes. Um maior calibre dos vasos sanguíneos foi observado em prenhez comparados às éguas cíclicas do dia 7 aos 13. No sétimo dia pós-ovulação, uma grande perda de células ciliadas foi evidente no grupo de éguas prenhes, comparadas ao grupo de éguas cíclicas, as células do epitélio endometrial estavam mais protusas e uma pequena quantidade de secreção histotrófica entre as dobras endometriais foi observada. No décimo dia de prenhez, secreção histotrófica glandular e do epitélio luminal estavam mais presentes comparadas às éguas do grupo cíclico. No dia 13 de prenhes, foi observado um grande conteúdo de histotrofo nas aberturas glandulares que estavam cercadas por células ciliares. Ocorreram alterações no ambiente uterino logo após a entrada do embrião no útero. No estroma e no lúmen, essas modificações parecem visar fornecer a nutrição necessária para o desenvolvimento inicial do embrião e estas mudanças nas estruturas celulares irão interagir na sinalização embrionária, futura fixação, implantação e placentação.

The early pregnancy of mare is a fascinating period that encompasses numerous and intense changes in its development, many of which are unique to the equine species. This development depends on the maintenance of the luteal function, the establishment of a favorable uterine environment and a precise and orchestrated interaction between the concept and the uterine environment. The aim of this study was to evaluate histological changes in the endometrium in days 7, 10 and 13 post-ovulation in pregnant and cyclic mares. In the first cycle, endometrial biopsies from 30 cyclic mares (Cyclic group) were collected on days 7, 10 and 13 post-ovulation. In the second cycle, the same mares were bred by a fertile stallion. At days 7, 10 and 13 post-ovulation intrauterine biopsies were collected. Immediately after sample collection, the mare‟s uteri were flushed, and those mares with embryo recovery were assigned to the Pregnant group. A larger blood vessel caliber was observed in pregnant mares than in cyclic from day 7 to…

Advisors/Committee Members: Gregory, Ricardo Macedo, Mattos, Rodrigo Costa.

Subjects/Keywords: Reprodução animal; Equinos; Desenvolvimento embrionário; Alterações fisiológicas; Endométrio; Equine embryo; Endometrium ultrastructure; Histotroph; Pre-implantation period; Ciliated cells; Blood vessel

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Camozzato, G. C. (2018). Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas. (Doctoral Dissertation). Brazil. Retrieved from http://hdl.handle.net/10183/183449

Chicago Manual of Style (16^th Edition):

Camozzato, Giovani Casanova. “Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas.” 2018. Doctoral Dissertation, Brazil. Accessed April 18, 2021. http://hdl.handle.net/10183/183449.

MLA Handbook (7^th Edition):

Camozzato, Giovani Casanova. “Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas.” 2018. Web. 18 Apr 2021.

Vancouver:

Camozzato GC. Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas. [Internet] [Doctoral dissertation]. Brazil; 2018. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10183/183449.

Council of Science Editors:

Camozzato GC. Características histológicas do endométrio durante o início do desenvolvimento embrionário em éguas. [Doctoral Dissertation]. Brazil; 2018. Available from: http://hdl.handle.net/10183/183449

University of Georgia

24. Xiao, Shuo. Molecular mechanism of embryo transport and embryo implantation in mice.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/29690

► Embryo transport and embryo implantation are essential events in mammalian reproduction during pregnancy. This dissertation was conducted to investigate the effect of bisphenol A (BPA)… (more)

Subjects/Keywords: Embryo transport; embryo implantation; preimplantation embryo development; fertility; bisphenol A; oviduct; uterus; uterine luminal epithelium; uterine receptivity; microarray analysis; vacuolar-type H+-ATPase; LE acidification; fat pad injection; N-acetylneuraminate pyruvate lyase; progesterone receptor.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Xiao, S. (2014). Molecular mechanism of embryo transport and embryo implantation in mice. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xiao, Shuo. “Molecular mechanism of embryo transport and embryo implantation in mice.” 2014. Thesis, University of Georgia. Accessed April 18, 2021. http://hdl.handle.net/10724/29690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xiao, Shuo. “Molecular mechanism of embryo transport and embryo implantation in mice.” 2014. Web. 18 Apr 2021.

Vancouver:

Xiao S. Molecular mechanism of embryo transport and embryo implantation in mice. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/10724/29690.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xiao S. Molecular mechanism of embryo transport and embryo implantation in mice. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29690

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Hoversland, Roger Carl. Implantation-associated protease in mouse uterine fluid.

Degree: PhD, 1980, Oregon Health Sciences University

URL: doi:10.6083/M4CF9N8K ; http://digitalcommons.ohsu.edu/etd/2319

Subjects/Keywords: Estrogens; Mice; Embryo Implantation; Ovum; Peptide Hydrolases; Progesterone; Uterus – secretion

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hoversland, R. C. (1980). Implantation-associated protease in mouse uterine fluid. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4CF9N8K ; http://digitalcommons.ohsu.edu/etd/2319

Chicago Manual of Style (16^th Edition):

Hoversland, Roger Carl. “Implantation-associated protease in mouse uterine fluid.” 1980. Doctoral Dissertation, Oregon Health Sciences University. Accessed April 18, 2021. doi:10.6083/M4CF9N8K ; http://digitalcommons.ohsu.edu/etd/2319.

MLA Handbook (7^th Edition):

Hoversland, Roger Carl. “Implantation-associated protease in mouse uterine fluid.” 1980. Web. 18 Apr 2021.

Vancouver:

Hoversland RC. Implantation-associated protease in mouse uterine fluid. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 1980. [cited 2021 Apr 18]. Available from: doi:10.6083/M4CF9N8K ; http://digitalcommons.ohsu.edu/etd/2319.

Council of Science Editors:

Hoversland RC. Implantation-associated protease in mouse uterine fluid. [Doctoral Dissertation]. Oregon Health Sciences University; 1980. Available from: doi:10.6083/M4CF9N8K ; http://digitalcommons.ohsu.edu/etd/2319

26. Carollo, James Robert. Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation.

Degree: MS, 1980, Oregon Health Sciences University

URL: doi:10.6083/M4J964KF ; http://digitalcommons.ohsu.edu/etd/2279

Subjects/Keywords: Cell Adhesion; Concanavalin A; Membrane Proteins; Mice – embryology; Embryo Implantation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carollo, J. R. (1980). Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation. (Thesis). Oregon Health Sciences University. Retrieved from doi:10.6083/M4J964KF ; http://digitalcommons.ohsu.edu/etd/2279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carollo, James Robert. “Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation.” 1980. Thesis, Oregon Health Sciences University. Accessed April 18, 2021. doi:10.6083/M4J964KF ; http://digitalcommons.ohsu.edu/etd/2279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carollo, James Robert. “Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation.” 1980. Web. 18 Apr 2021.

Vancouver:

Carollo JR. Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation. [Internet] [Thesis]. Oregon Health Sciences University; 1980. [cited 2021 Apr 18]. Available from: doi:10.6083/M4J964KF ; http://digitalcommons.ohsu.edu/etd/2279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carollo JR. Changes in binding of [Â³H] Concanavalin A to mouse blastocysts at implantation. [Thesis]. Oregon Health Sciences University; 1980. Available from: doi:10.6083/M4J964KF ; http://digitalcommons.ohsu.edu/etd/2279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Werken, Christine. Epigenetics and chromosome segregation in human pre-implantation embryos.

Degree: 2015, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/78695

► markdownabstractAbstract Chapter 1 Currently, the average pregnancy rate per embryo transfer after in vitro fertilization (IVF) is around 32%. In order to achieve better results… (more)

▼ markdownabstractAbstract Chapter 1 Currently, the average pregnancy rate per embryo transfer after in vitro fertilization (IVF) is around 32%. In order to achieve better results in the future, we need to gain knowledge on all aspects of the treatment, including pre-implantation embryo development. In this thesis, we describe the research we performed into epigenetics and chromosome segregation in human pre-implantation embryos derived from IVF. The term ‘epigenetics’ refers to heritable marks on the genome, such as DNA methylation and histone modifications. These marks are essential for chromosome structure, chromosome segregation and gene expression. Chromosome segregation is the process in which duplicated chromosomes are equally separated over two cells during cell division. Chromosomal abnormalities are detected at high frequencies in human pre-implantation embryos. This suggests that mechanisms regulating chromosome segregation are less functional during the first cell divisions of an embryo. Next to that, epigenetic marks, which are also important for correct chromosome segregation, are different in oocytes and spermatozoa and need to be re-established in early embryos. The research described in this thesis aimed to investigate both the mechanisms regulation chromosome segregation and the re-establishment of epigenetics marks in human pre-implantation embryos, in order to shed light on the causes of chromosomal abnormalities. Chapter 2 DNA is wrapped around histones, together forming chromatin. Epigenetic marks like histone modifications determine the structure of chromatin and define certain chromatin domains. Oocytes and spermatozoa have a very different chromatin structure, both from each other and from somatic cells, and after fertilization, canonical chromatin domains need to be re-established. In this chapter we investigated the re-establishment of a chromatin domain important for chromosome segregation, constitutive heterochromatin (cHC), in human pre-implantation embryos derived from IVF. We describe that human spermatozoa carry histones with canonical cHC modifications (H3K9me3, H4K20me3, H3K64me3). After fertilization, these modified histones contribute to the formation of paternal embryonic cHC. The histone modifications are recognized by maternal chromatin regulators (e.g. HP1, SUV39H) and propagated over the embryonic cell divisions. These results indicate transgenerational epigenetic inheritance of cHC structure in human embryos and show that there is an important contribution of the spermatozoon to embryo development. Until now, this process was studied only in mouse embryos, in which paternal cHC in spermatozoa and embryos lacks canonical modifications and is transiently established by other mechanisms, present in the oocyte. Often these results are assumed to be applicable for all mammalian species. However, we now show that the mechanism in human embryos differs significantly from what has been described for mice. This points out that mouse embryos are not a representative…

Subjects/Keywords: Human pre-implantation embryo; Chromosome segregation; Epigenetics; Chromatin; Immunofluorescence microscopy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Werken, C. (2015). Epigenetics and chromosome segregation in human pre-implantation embryos. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/78695

Chicago Manual of Style (16^th Edition):

Werken, Christine. “Epigenetics and chromosome segregation in human pre-implantation embryos.” 2015. Doctoral Dissertation, Erasmus University Medical Center. Accessed April 18, 2021. http://hdl.handle.net/1765/78695.

MLA Handbook (7^th Edition):

Werken, Christine. “Epigenetics and chromosome segregation in human pre-implantation embryos.” 2015. Web. 18 Apr 2021.

Vancouver:

Werken C. Epigenetics and chromosome segregation in human pre-implantation embryos. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 2015. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/1765/78695.

Council of Science Editors:

Werken C. Epigenetics and chromosome segregation in human pre-implantation embryos. [Doctoral Dissertation]. Erasmus University Medical Center; 2015. Available from: http://hdl.handle.net/1765/78695

28. MEHRAN RAHMANI. PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE.

Degree: 2014, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/118131

Subjects/Keywords: Embryo implantation; syncytiotrophoblast; Placenta evolution; Primate-specific; Genomic technologies; Human Embryonic Stem Cell

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

RAHMANI, M. (2014). PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/118131

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

RAHMANI, MEHRAN. “PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE.” 2014. Thesis, National University of Singapore. Accessed April 18, 2021. http://scholarbank.nus.edu.sg/handle/10635/118131.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

RAHMANI, MEHRAN. “PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE.” 2014. Web. 18 Apr 2021.

Vancouver:

RAHMANI M. PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE. [Internet] [Thesis]. National University of Singapore; 2014. [cited 2021 Apr 18]. Available from: http://scholarbank.nus.edu.sg/handle/10635/118131.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

RAHMANI M. PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE. [Thesis]. National University of Singapore; 2014. Available from: http://scholarbank.nus.edu.sg/handle/10635/118131

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

29. Ramathal, Cyril Y. Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy.

Degree: PhD, 4094, 2010, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/15569

► Embryo implantation into the endometrium is a complex biological process involving the integration of steroid hormone signaling, endometrial tissue remodeling and maternal- fetal communications. A… (more)

▼ Embryo implantation into the endometrium is a complex biological process involving the integration of steroid hormone signaling, endometrial tissue remodeling and maternal- fetal communications. A successful pregnancy is the outcome of the timely integration of these events during the early stages of implantation. The involvement of ovarian steroid hormones, estrogen (E) and progesterone (P), acting through their cognate receptors, is essential for uterine functions during pregnancy. The molecular mechanisms that control the process of implantation are undergoing active exploration. Through our recent efforts, we identified the transcription factor, CCAAT Enhancer Binding Protein Beta (C/EBPb) as a prominent target of estrogen and progesterone signaling in the uterus. The development of a C/EBPb-null mouse model, which is infertile, presented us with an opportunity to analyze the role of this molecule in uterine function. We discovered that C/EBPb functions in two distinct manners: (i) by acting as a mediator of E-induced proliferation of the uterine epithelium and (ii) by controlling uterine stromal cell differentiation, a process known as decidualization, during pregnancy. My studies have delineated important mechanisms by which E regulates C/EBPb expression to induce DNA replication and prevent apoptosis of uterine epithelial cells during E-induced epithelial growth. In subsequent studies, I analyzed the role of C/EBPb in decidualization and uncovered a unique mechanism by which C/EBPb regulates the synthesis of a unique laminin-containing extracellular matrix (ECM) that supports stromal cell differentiation and embryo invasion. In order to better define the role of laminin in implantation, we developed a laminin gamma 1-conditional knockout mouse model. This is currently an area of ongoing investigation. The information gained from our analysis of C/EBPb function in the uterus provides new insights into the mechanisms of steroid hormone action during early pregnancy. Ultimately, our findings may aid in the understanding of dysregulation of hormone-controlled pathways that underlie early pregnancy loss and infertility in women. Advisors/Committee Members: Bagchi, Milan K. (advisor), Katzenellenbogen, Benita S. (Committee Chair), Bagchi, Milan K. (committee member), Kemper, Byron W. (committee member), Freeman, Brian C. (committee member), Raetzman, Lori T. (committee member).

Subjects/Keywords: Implantation; Infertility; decidualization; embryo; uterus; endometrium; estrogen; progesterone; Extracellular Matrix (ECM)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramathal, C. Y. (2010). Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/15569

Chicago Manual of Style (16^th Edition):

Ramathal, Cyril Y. “Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy.” 2010. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 18, 2021. http://hdl.handle.net/2142/15569.

MLA Handbook (7^th Edition):

Ramathal, Cyril Y. “Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy.” 2010. Web. 18 Apr 2021.

Vancouver:

Ramathal CY. Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2010. [cited 2021 Apr 18]. Available from: http://hdl.handle.net/2142/15569.

Council of Science Editors:

Ramathal CY. Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2010. Available from: http://hdl.handle.net/2142/15569

30. Porchet, Nicolas. Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris.

Degree: Docteur es, Sciences de la vie et de la santé, 2019, Université de Paris (2019-....)

URL: http://www.theses.fr/2019UNIP7096

►

Lors du développement précoce de l’embryon de souris, divers évènements de spécification des destins cellulaires induisent la formation du blastocyste pré-implantatoire. Ces évènements sont majoritairement… (more)

Subjects/Keywords: Souris; Embryon; Pré-implantatoire; Blastocyste; Destins; Spécification; ACTIVIN/NODAL; ΒCATENIN; Signalisation; Voies; Mouse; Embryo; Pre-implantation; Blastocyst; Cell-fate; Specification; ACTIVIN/NODAL; ΒCATENIN; Signaling; Pathways

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Porchet, N. (2019). Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris. (Doctoral Dissertation). Université de Paris (2019-....). Retrieved from http://www.theses.fr/2019UNIP7096

Chicago Manual of Style (16^th Edition):

Porchet, Nicolas. “Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris.” 2019. Doctoral Dissertation, Université de Paris (2019-....). Accessed April 18, 2021. http://www.theses.fr/2019UNIP7096.

MLA Handbook (7^th Edition):

Porchet, Nicolas. “Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris.” 2019. Web. 18 Apr 2021.

Vancouver:

Porchet N. Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris. [Internet] [Doctoral dissertation]. Université de Paris (2019-....); 2019. [cited 2021 Apr 18]. Available from: http://www.theses.fr/2019UNIP7096.

Council of Science Editors:

Porchet N. Role of signaling pathays in cell-fate specification in the early mouse embryo : Rôle des voies de signalisation dans la spécification des destins cellulaires chez l’embryon précoce de souris. [Doctoral Dissertation]. Université de Paris (2019-....); 2019. Available from: http://www.theses.fr/2019UNIP7096

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations