subject:(electroporation)

Rutgers University

1. Zheng, Mingde, 1987-. Development of a continuous-flow, automated microfluidic device for single cell level electroporation.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

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Electroporation is a powerful transfection technique that creates transient openings in the cell membrane by applying an electric field, allowing for the intracellular delivery of… (more)

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Electroporation is a powerful transfection technique that creates transient openings in the cell membrane by applying an electric field, allowing for the intracellular delivery of diagnostic and therapeutic substances. The ability to detect and control the degree of cell membrane permeability plays a key role in determining the size of the delivery payload, while safeguarding the overall cell viability. In order to create a universal electroporation system, this dissertation describes the development of a continuous flow electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. In contrast to devices that immobilize individual cells for impedance analysis, this work demonstrates the capability to manipulate single cells under flow and real-time analysis of membrane permeabilization before and after electroporation, which dramatically increasing the number of cells which can be electroporated and analyzed. Using an electric circuit model, and Multiphysics computational tools, the key parameters for successful cell membrane permeabilization detection in a flow environment were determined. By varying the electric field parameters, we demonstrate the direct control of cell membrane permeabilization by electrically measuring the electroporation-induced cell membrane impedance change and by optically measuring the delivery of a fluorescent probe. Viability of the electroporated cells following collection also demonstrates a correlation with the applied pulse strength. By extending the device capability to include dynamic pulse adjustment according to the real-time feedback information on cell viability, an intelligent electroporation system capable of potentially maximizing delivery efficiency and cell viability can be thus realized.

Advisors/Committee Members: Zahn, Jeffrey D. (chair), Shreiber, David I. (internal member), Tischfield, Jay A. (internal member), Lin, Hao (outside member), Shan, Jerry W. (outside member).

Subjects/Keywords: Electroporation

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APA (6^th Edition):

Zheng, Mingde, 1. (2016). Development of a continuous-flow, automated microfluidic device for single cell level electroporation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

Chicago Manual of Style (16^th Edition):

Zheng, Mingde, 1987-. “Development of a continuous-flow, automated microfluidic device for single cell level electroporation.” 2016. Doctoral Dissertation, Rutgers University. Accessed February 27, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51538/.

MLA Handbook (7^th Edition):

Zheng, Mingde, 1987-. “Development of a continuous-flow, automated microfluidic device for single cell level electroporation.” 2016. Web. 27 Feb 2021.

Vancouver:

Zheng, Mingde 1. Development of a continuous-flow, automated microfluidic device for single cell level electroporation. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2021 Feb 27]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/.

Council of Science Editors:

Zheng, Mingde 1. Development of a continuous-flow, automated microfluidic device for single cell level electroporation. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

Rutgers University

2. Hogquist, Stephen Joseph, 1995-. Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates.

Degree: MS, Biomedical Engineering, 2020, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/62544/

► Electroporation is the technique of applying an electric field to a cell to achieve temporary membrane permeabilization and allow the passage of drugs, chemicals, or… (more)

▼ Electroporation is the technique of applying an electric field to a cell to achieve temporary membrane permeabilization and allow the passage of drugs, chemicals, or DNA through the compromised cell membrane. Electroporation technology is presently an area of research interest due to its versatility and potential benefit to various investigational therapies including vaccines and cell and gene therapies. The transfection efficiency and cell viability following electroporation experiments depend strongly on various parameters such as the applied electric field, cell type used, and the contents and electrical characteristics of the buffer solution through which a field is applied. This study aims to demonstrate a technique by which a lower voltage can be used to electroporate cells with the goal of increasing cell viability and permeabilization efficiency with plasmid DNA and propidium iodide when compared to existing techniques. This is achieved utilizing 3T3 cells attached to a biocompatible, 60µm thick nanoporous alumina membrane substrate with 100nm pores. Computational modeling predicts that applying an electric field through this nanoporous substrate in a conductive buffer solution results in electric field amplification near nanopores to achieve electroporation of cells adherent to the substrate, while maintaining a lower field strength elsewhere in the remaining area surrounding a cell. As many existing electroporation experiments work with cell suspensions in cuvettes to achieve electroporation, this technique presents a method to achieve electroporation of adherent cells. Results suggest that delivery cargo of interest can be electrophoretically driven through these nanopores and enter a cell with an electroporation pulse under select electric field conditions. Here, a technique is described to further investigate the application of nanoporous substrates in electroporation experiments. Furthermore, the impact of electric field strength amplification through alumina nanopores is demonstrated by the successful permeabilization of cell membranes with delivery markers. Advisors/Committee Members: Zahn, Jeffrey D (chair), Shreiber, David (internal member), Labazzo, Kristen (internal member), School of Graduate Studies.

Subjects/Keywords: Electroporation

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APA (6^th Edition):

Hogquist, Stephen Joseph, 1. (2020). Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/62544/

Chicago Manual of Style (16^th Edition):

Hogquist, Stephen Joseph, 1995-. “Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates.” 2020. Masters Thesis, Rutgers University. Accessed February 27, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/62544/.

MLA Handbook (7^th Edition):

Hogquist, Stephen Joseph, 1995-. “Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates.” 2020. Web. 27 Feb 2021.

Vancouver:

Hogquist, Stephen Joseph 1. Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates. [Internet] [Masters thesis]. Rutgers University; 2020. [cited 2021 Feb 27]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62544/.

Council of Science Editors:

Hogquist, Stephen Joseph 1. Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates. [Masters Thesis]. Rutgers University; 2020. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/62544/

Rutgers University

3. Yu, Miao, 1984-. A model study of molecular transport in electroporation.

Degree: PhD, Mechanical and Aerospace Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

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Reversible electroporation is a non-viral technique to introduce foreign molecules into biological cells or tissues, which has found applications in fields including gene transfer, cancer… (more)

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Reversible electroporation is a non-viral technique to introduce foreign molecules into biological cells or tissues, which has found applications in fields including gene transfer, cancer treatment, stem-cell research etc. Despite its promising potential, the improvement of electroporation technique is impeded by the lack of a comprehensive understanding of the underlying mechanisms involved in the process of molecular delivery. This work aims at implementing model studies of electroporation-mediated molecular delivery with the target varying from small molecules (propidium iodide, PI) to macromolecules (DNA). Three significant tasks have been accomplished. First, a model study is performed on the electroporation-mediated delivery of PI. In particular, the effects of extra-cellular conductivity on the amount of PI delivery are carefully investigated and discussed. The results are extensively compared with experiments by Sadik et al., and reveal important physical insights about the transport mechanisms involved. It is confirmed that the electrophoretic transport, not the diffusive transport, is the dominating mechanism in mediating PI delivery, and the inverse correlation observed between PI delivery and extra-cellular conductivity results from an electrokinetic phenomenon termed Field Amplified Sample Stacking (FASS). Second, a model investigation of Fluorescein-Dextran delivery is implemented for double-pulse electroporation. Simulated results find qualitative agreement with experiments in predicting the correlation between delivery and pulsing parameters. A bifurcation analysis of equilibrium pore size with respect to the transmembrane potential is presented to explain the observed “critical field strength” above which the second pulse abruptly becomes effective in mediating delivery. Third, a 1D Fokker-Planck simulation is used to characterize the process of DNA translocation through an electropore under finite DC pulses. It is found that the translocation may occur on two disparate time scales, the electrophoretic time (~ ms), and the diffusive time (~ s), depending on the pulse length. Furthermore, a power-law correlation is observed between the final probability of successful translocation and pulsing parameters. Simulated results are compared with previous data to interpret the trends, and further model predictions are made which can be verified by well-designed experiments. Together, these projects establish connections between available theoretical model and experimental observations in electroporation research. Such a connection on one hand benefits experimentalists in providing a powerful prediction tool for the design and optimization of electroporation; on the other hand it equally benefits theorists to improve the models and advance fundamental understandings in the subject.

Advisors/Committee Members: Lin, Hao (chair), Shan, Jerry (internal member), Bagchi, Prosenjit (internal member), Shreiber, David (outside member).

Subjects/Keywords: Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, Miao, 1. (2014). A model study of molecular transport in electroporation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

Chicago Manual of Style (16^th Edition):

Yu, Miao, 1984-. “A model study of molecular transport in electroporation.” 2014. Doctoral Dissertation, Rutgers University. Accessed February 27, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/45583/.

MLA Handbook (7^th Edition):

Yu, Miao, 1984-. “A model study of molecular transport in electroporation.” 2014. Web. 27 Feb 2021.

Vancouver:

Yu, Miao 1. A model study of molecular transport in electroporation. [Internet] [Doctoral dissertation]. Rutgers University; 2014. [cited 2021 Feb 27]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/.

Council of Science Editors:

Yu, Miao 1. A model study of molecular transport in electroporation. [Doctoral Dissertation]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

Université de Neuchâtel

4. Generelli, Silvia. Ion-selective microelectrode arrays for cell culture monitoring.

Degree: 2008, Université de Neuchâtel

URL: http://doc.rero.ch/record/10685

► The design, microfabrication and characterization of a platform comprising an array of ion-selective microelectrodes (µISE) aimed at <i>in vitro</i> cellular physiology and toxicology is described.… (more)

▼ The design, microfabrication and characterization of a platform comprising an array of ion-selective microelectrodes (µISE) aimed at <i>in vitro</i> cellular physiology and toxicology is described. This study focusses on K⁺ and Ca²⁺ monitoring in cell culture environments. A potential promising application of such a platform is based on recent findings in molecular biology, revealing connections between certain diseases, as for example some types of cancer or parkinsonism, and a malfunction in cellular ion fluxes. The silicon microfabrication of the platform allowed the realization of ion-selective microelectrode arrays comprising 16 electrodes with diameters ranging from 1.5 µm to 6 µm, and an interelectrode gap of 150 µm or 300 µm. The µISE, inspired by the classical glass capillary microelectrodes, are based on a micropipette-like channel which, in two of the three realized geometries, protrude for 5 µm from the surface and are located in a 350 µm deep cavity. In the third geometry, the micropipettes are non-protruding, completely embedded in the silicon substrate. This planar platform allowed the implementation of an array of K⁺-selective electrodes combined with planar platinum electrodes. The metallic electrodes placed near the ion-selective electrodes are designed for the application of electrical pulses for localised electroporation of cells. Preliminary tests showed the feasibility of localised electroporation, and demonstrated that the application of several electroporation pulses does not affect the functionality of K⁺ selective microelectrodes, however further investigation is necessary to optimize the electroporation protocol. The characterization of the electrodes in physiological concentration ranges was performed using electrodes of 1.5 µm diameter in contact with simple buffer calibration solutions. The observed detection limits, 10^-5 M for the K⁺ -selective electrodes and 10^-9 M for the Ca<sup²⁺ -selective electrodes, demonstrated that the µISE are adapted for the use in cell culture concentration ranges. The functional lifetime of the sensors, when conditioned in 10^-3 M KCl, or 10^-4 M CaCl₂ respectively, varied from 30 to 45 days for K⁺ -selective electrodes, but is limited to 1 to 3 days for Ca²⁺-selective electrodes. This difference in the functional lifetimes of K⁺- and Ca²⁺ -selective electrodes is due to the use of calcium-selective sensors in their non-equilibrium state. After several days of use the calcium detection limit rises to the micromolar range, which is adapted for extracellular calcium concentrations, but not for intracellular monitoring. In this case, the lifetime of the sensors is comparable to those of K⁺-selective microelectrodes. Tests performed in presence of culture medium showed a drastic diminution of the electrodes lifetime down to several days. On the other hand, a short-time contact of the sensors… Advisors/Committee Members: Nicolas-F. (Dir.).

Subjects/Keywords: electroporation

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APA (6^th Edition):

Generelli, S. (2008). Ion-selective microelectrode arrays for cell culture monitoring. (Thesis). Université de Neuchâtel. Retrieved from http://doc.rero.ch/record/10685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Generelli, Silvia. “Ion-selective microelectrode arrays for cell culture monitoring.” 2008. Thesis, Université de Neuchâtel. Accessed February 27, 2021. http://doc.rero.ch/record/10685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Generelli, Silvia. “Ion-selective microelectrode arrays for cell culture monitoring.” 2008. Web. 27 Feb 2021.

Vancouver:

Generelli S. Ion-selective microelectrode arrays for cell culture monitoring. [Internet] [Thesis]. Université de Neuchâtel; 2008. [cited 2021 Feb 27]. Available from: http://doc.rero.ch/record/10685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Generelli S. Ion-selective microelectrode arrays for cell culture monitoring. [Thesis]. Université de Neuchâtel; 2008. Available from: http://doc.rero.ch/record/10685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

5. Shagoshtasbi, Hooman. Systematic design and fabrication of a smart microfluidic single-cell electroporator.

Degree: 2013, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-86314 ; https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

► A nonlinear dynamic electro-mechanical model for a single-cell electroporation device (EP) is proposed based on micro EP experimental results. This model considers the behavior of… (more)

▼ A nonlinear dynamic electro-mechanical model for a single-cell electroporation device (EP) is proposed based on micro EP experimental results. This model considers the behavior of electromechanical components of a biological cell, the electric components of the media together with the device’s electrical components. There are four state variables for the proposed EP model including the voltage across the micro electrodes for EP, the transmembrane voltage, the average radius of pores and the cell membrane pore density. The numerical simulation of the model demonstrates good agreement with the experimental data. The predicted current responses show a much better agreement (8% error) with the micro EP experiments in comparison with the previous models. The model predicts the critical transmembrane voltage which is in good agreement with previous derived values from experiments. In addition, the proposed model can explain various aspects of structural changes of the cell membrane during different stages of electroporation, such as the Current-Voltage (I-V) characteristics of the cell membrane, the number of electropores and average pore radius. The numerical I-V characteristics of a nanoscale electropore on the cell membrane during EP illustrates the formation and expansion of the electropore during different stages of EP is analogous to the electric breakdown of the PNP junction in a semiconductor DIAC (diode for alternating current). The proposed model illustrates that the cell size is important to determine the system specifications. Based on the proposed EP model, a single-cell EP system is designed and fabricated to determine the cell size using on-chip micro Coulter counter and to apply the optimal electric field to achieve high EP efficiency and cell viability. The fabricated single-cell EP microchip is tested using HeLa cells. An EP electric field optimizer microchip is designed and fabricated to derive the optimal applied electric field for different sizes of the suspended HeLa cells. Compare to commercialized Electroporators, the fabricated device reduces the required applied voltage from several kV to several volts. The viability and permeability of HeLa cell line for the typical macro EP system are 50% and 65% respectively. For the open loop micro EP systems, the typical viable and permeabilized HeLa cells for applied electric field of 1.1 kV/cm are 76% and 75% respectively. The smart closed loop micro EP system improves the viability and permeabilization of cells to more than 90%.

Subjects/Keywords: Electroporation ; Microfluidics

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APA (6^th Edition):

Shagoshtasbi, H. (2013). Systematic design and fabrication of a smart microfluidic single-cell electroporator. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-86314 ; https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shagoshtasbi, Hooman. “Systematic design and fabrication of a smart microfluidic single-cell electroporator.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed February 27, 2021. http://repository.ust.hk/ir/Record/1783.1-86314 ; https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shagoshtasbi, Hooman. “Systematic design and fabrication of a smart microfluidic single-cell electroporator.” 2013. Web. 27 Feb 2021.

Vancouver:

Shagoshtasbi H. Systematic design and fabrication of a smart microfluidic single-cell electroporator. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2021 Feb 27]. Available from: http://repository.ust.hk/ir/Record/1783.1-86314 ; https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shagoshtasbi H. Systematic design and fabrication of a smart microfluidic single-cell electroporator. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: http://repository.ust.hk/ir/Record/1783.1-86314 ; https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cal Poly

6. Foltz, Garrett. Algae Lysis with Pulsed Electric Fields.

Degree: MS, Electrical Engineering, 2012, Cal Poly

URL: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

► With growing interest in alternative fuels, algae lipid harvesting is seen as a possible source of biofuel. Algae species under consideration include Chlorella vulgaris,… (more)

Subjects/Keywords: Biofuel; Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Foltz, G. (2012). Algae Lysis with Pulsed Electric Fields. (Masters Thesis). Cal Poly. Retrieved from https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

Chicago Manual of Style (16^th Edition):

Foltz, Garrett. “Algae Lysis with Pulsed Electric Fields.” 2012. Masters Thesis, Cal Poly. Accessed February 27, 2021. https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45.

MLA Handbook (7^th Edition):

Foltz, Garrett. “Algae Lysis with Pulsed Electric Fields.” 2012. Web. 27 Feb 2021.

Vancouver:

Foltz G. Algae Lysis with Pulsed Electric Fields. [Internet] [Masters thesis]. Cal Poly; 2012. [cited 2021 Feb 27]. Available from: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45.

Council of Science Editors:

Foltz G. Algae Lysis with Pulsed Electric Fields. [Masters Thesis]. Cal Poly; 2012. Available from: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

Rutgers University

7. Li, Jianbo, 1980-. Modeling of electroporation mediated molecular delivery.

Degree: Mechanical and Aerospace Engineering, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Subjects/Keywords: Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Jianbo, 1. (2011). Modeling of electroporation mediated molecular delivery. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Jianbo, 1980-. “Modeling of electroporation mediated molecular delivery.” 2011. Thesis, Rutgers University. Accessed February 27, 2021. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Jianbo, 1980-. “Modeling of electroporation mediated molecular delivery.” 2011. Web. 27 Feb 2021.

Vancouver:

Li, Jianbo 1. Modeling of electroporation mediated molecular delivery. [Internet] [Thesis]. Rutgers University; 2011. [cited 2021 Feb 27]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li, Jianbo 1. Modeling of electroporation mediated molecular delivery. [Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

8. Kim, Seunghyun. Investigation of Mechanisms of Electroporation of Lipid Bilayers.

Degree: MS, Chemical Engineering, 2018, University of Florida

URL: https://ufdc.ufl.edu/UFE0052296

► Electroporation, i.e. formation of pores in cellular membrane by application of an external electric field, is a common technique used for introducing various substances into… (more)

Subjects/Keywords: electroporation – pore-formation

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APA (6^th Edition):

Kim, S. (2018). Investigation of Mechanisms of Electroporation of Lipid Bilayers. (Masters Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0052296

Chicago Manual of Style (16^th Edition):

Kim, Seunghyun. “Investigation of Mechanisms of Electroporation of Lipid Bilayers.” 2018. Masters Thesis, University of Florida. Accessed February 27, 2021. https://ufdc.ufl.edu/UFE0052296.

MLA Handbook (7^th Edition):

Kim, Seunghyun. “Investigation of Mechanisms of Electroporation of Lipid Bilayers.” 2018. Web. 27 Feb 2021.

Vancouver:

Kim S. Investigation of Mechanisms of Electroporation of Lipid Bilayers. [Internet] [Masters thesis]. University of Florida; 2018. [cited 2021 Feb 27]. Available from: https://ufdc.ufl.edu/UFE0052296.

Council of Science Editors:

Kim S. Investigation of Mechanisms of Electroporation of Lipid Bilayers. [Masters Thesis]. University of Florida; 2018. Available from: https://ufdc.ufl.edu/UFE0052296

Universidade Nova

9. Fernandes, Catarina Sofia Rodrigues. Linking the embryonic clock with temporal collinearity of Hox gene activation.

Degree: 2010, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

►

Dissertação apresentada para a obtenção do Grau de Mestre em Genética Molecular e Biomedicina, pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Somitogenesis… (more)

Subjects/Keywords: Hairy1; Brachyury; Electroporation; Molecular clock

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APA (6^th Edition):

Fernandes, C. S. R. (2010). Linking the embryonic clock with temporal collinearity of Hox gene activation. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernandes, Catarina Sofia Rodrigues. “Linking the embryonic clock with temporal collinearity of Hox gene activation.” 2010. Thesis, Universidade Nova. Accessed February 27, 2021. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernandes, Catarina Sofia Rodrigues. “Linking the embryonic clock with temporal collinearity of Hox gene activation.” 2010. Web. 27 Feb 2021.

Vancouver:

Fernandes CSR. Linking the embryonic clock with temporal collinearity of Hox gene activation. [Internet] [Thesis]. Universidade Nova; 2010. [cited 2021 Feb 27]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernandes CSR. Linking the embryonic clock with temporal collinearity of Hox gene activation. [Thesis]. Universidade Nova; 2010. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

10. Riaz, Kashif MAE. 3D nano-spikes based system for biological manipulation and sensing at low voltages.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-100478 ; https://doi.org/10.14711/thesis-b1627881 ; http://repository.ust.hk/ir/bitstream/1783.1-100478/1/th_redirect.html

► I developed 3D nano-spikes based system for biological manipulations and sensing, such as electroporation (EP), electric cell lysis (ECL), electroporation based protein extraction and impedance… (more)

▼ I developed 3D nano-spikes based system for biological manipulations and sensing, such as electroporation (EP), electric cell lysis (ECL), electroporation based protein extraction and impedance based cancer detection. Electrical methods are used for biological manipulations and sensing with low energy consumption due to electric field enhancement at 3D high-aspect-ratio nano-spikes (NSPs). Periodic 3D high-aspect-ratio nano-spikes were fabricated on low cost commercial Al foils using scalable electrochemical anodization and etching processes with controllable dimensions ranging from 600 nm to 1100 nm. Due to scalability of fabrication process, 3D NSPs were fabricated on microchips as well as on a 4 inch wafer. High EP efficiencies and cell viabilities (>93±6%) for HeLa cells were achieved at 4 V for single pulse of 2ms and at 2V, 12 ms for 10 pulses on nano-spikes based electroporation (NSP-EP) chips by optimizing electric pulse protocol and NSPs dimensions. This applied voltage is more than ten times lower in comparison with planar electroporation (PEP) devices without NSPs. Low voltage operation avoided bubble generation on chips and increase device reliability and cell viabilities. I have developed an energy efficient 3D high-aspect-ratio nano-spikes based electric cell lysis (NSP-ECL) chips for efficient cell lysis due to electric field enhancement at NSPs. NSP-ECL chips have achieved high cell lysis efficiencies ƞ_lysis (99.9±0.1%) at more than ten times reduced pulse amplitudes (2 V) in comparison to the planar ECL chips without NSPs. These applied pulse amplitudes are 2-3 times reduced in comparison with traditional electroporation systems. The specific energy input required to achieve 99.9±0.1% ƞ_lysis was only in the range of 0.5-2 mJ/mL which is 3-9 orders of magnitude lower in comparison with other cell disintegration methods (5J/mL-540kJ/mL). I have developed an energy efficient method for intracellular protein extraction using electroporation on 3D nano-spikes with minimum cell invasiveness. The specific energy input required for protein extraction was in the range of 0.5-3 mJ/mL which is 4-8 orders of magnitude less than other classical methods. Cell membrane disintegration, cell debris micronization, and non-selective contaminant release was avoided durin protein extraction through reversible electroporation. I have developed 3D nano-spikes based Bio-impedance senor (nBIS) for label-free impedance detection and phenotyping of cancer and non-cancer cells. Electric signals were extracted directly from cell membrane by nano-spikes penetration into the cells. Charge transfer resistance R_ct was good electric indicator with 20-30% difference for detection and phenotyping of different cells.

Subjects/Keywords: Electroporation ; Biosensors ; Bioelectrochemistry ; Nanotechnology

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APA (6^th Edition):

Riaz, K. M. (2016). 3D nano-spikes based system for biological manipulation and sensing at low voltages. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-100478 ; https://doi.org/10.14711/thesis-b1627881 ; http://repository.ust.hk/ir/bitstream/1783.1-100478/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Riaz, Kashif MAE. “3D nano-spikes based system for biological manipulation and sensing at low voltages.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed February 27, 2021. http://repository.ust.hk/ir/Record/1783.1-100478 ; https://doi.org/10.14711/thesis-b1627881 ; http://repository.ust.hk/ir/bitstream/1783.1-100478/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Riaz, Kashif MAE. “3D nano-spikes based system for biological manipulation and sensing at low voltages.” 2016. Web. 27 Feb 2021.

Vancouver:

Riaz KM. 3D nano-spikes based system for biological manipulation and sensing at low voltages. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Feb 27]. Available from: http://repository.ust.hk/ir/Record/1783.1-100478 ; https://doi.org/10.14711/thesis-b1627881 ; http://repository.ust.hk/ir/bitstream/1783.1-100478/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Riaz KM. 3D nano-spikes based system for biological manipulation and sensing at low voltages. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-100478 ; https://doi.org/10.14711/thesis-b1627881 ; http://repository.ust.hk/ir/bitstream/1783.1-100478/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

11. Movahed, Saeid. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/7149

► In this thesis, underlying concepts of transport phenomena through generated nanopores on a cell membrane during electroporation were studied. A comprehensive literature review was performed… (more)

▼ In this thesis, underlying concepts of transport phenomena through generated nanopores on a cell membrane during electroporation were studied. A comprehensive literature review was performed to find the pros and cons of the previous works and consequently extensive studies were accomplished to explain shortcomings of the former studies on this topic. The membrane permeabilization of the single cell located in the microchannel was studied, and the effects of microchannel’s wall and electrode size were investigated on cell electroporation. It was studied how the electrical (e.g., strength of the electric pulse) and geometrical parameters (e.g., microchannel height and electrode size) affect size, location, and number of created hydrophilic pores on the cell membrane. Because of a transmembrane potential, the electrokinetic effects have decisive influence on the transport process through the created nanopores. A comprehensive study was performed to explain the electrokinetic transport through the nanochannels. Effects of surface electric charge and radius of the nanochannel on the electric potential, liquid flow, and ionic transport were investigated. Unlike microchannels, the electric potential field, ionic concentration field, and velocity field are strongly size-dependent in the nanochannels. They are also affected by the surface electric charge of the nanochannel. More counter ions than co-ions are transported through the nanochannel. The ionic concentration enrichment at the entrance and the exit of the nanochannel is completely evident from the simulation results. The study also shows that the fluid velocity in the nanochannel is higher when the surface electric charge is stronger, or the radius of the nanochannel is larger. The obtained model of the electrokinetic effects in the nanochannels was utilized to examine the ionic mass transfer and the fluid flow through the generated hydrophilic nanopores of the cell membrane during electroporation. The results showed how the electric potential, velocity field, and ionic concentration vary with the size and angular position of the generated nanopores of the cell membrane. It was also shown that, in the presence of the electric pulse, the electrokinetic effects (the electroosmosis and the electrophoresis) had significant influences on the ionic mass transfer through the nanopores, while the effect of diffusion on the ionic mass flux was negligible in comparison with the electrokinetics. Increasing the radius of the nanopores intensified the effect of convection (electroosmosis) in comparison with the electrophoresis on the ionic flux. Furthermore, the electrokinetic motion of the nanoparticle through the nanochannel was investigated to mimic inserting the nanoscale biological samples, such as QDots and DNAs, through the created nanopores on the cell membrane. It was proved that, because of the large applied electric field over the nanochannel, the impact of the Brownian force was negligible in comparison with the electrophoretic and the hydrodynamic forces. It…

Subjects/Keywords: electroporation; electrokinetics; nanopore; nanochannel

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APA (6^th Edition):

Movahed, S. (2012). Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Movahed, Saeid. “Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.” 2012. Thesis, University of Waterloo. Accessed February 27, 2021. http://hdl.handle.net/10012/7149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Movahed, Saeid. “Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.” 2012. Web. 27 Feb 2021.

Vancouver:

Movahed S. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10012/7149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Movahed S. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

12. Sano, Michael B. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.

Degree: PhD, Biomedical Engineering, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/77122

► The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare… (more)

▼ The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare mammalian cells dispersed within heterogeneous populations. Relevant examples of these target cells are stem cells within a differentiated population, circulating tumor cells (CTCs) in the blood stream, and tumor initiating cells (TICs) in a population of benign cancer cells. The ability to isolate any of these rare cells types with high efficiency will directly lead to advances in tissue engineering, cancer detection, and individualized medicine. This work lead directly to the development of a new cell manipulation technique, termed contactless dielectrophoresis (cDEP). In this technique, cells are isolated from direct contact with metal electrodes by employing fluid electrode channels filled with a highly conductive media. Thin insulating barriers, approximately 20 μm, serve to isolate the fluid electrode channels from the low conductivity sample buffer. The insulating barriers in a fluid-electrical system create a number of unique and interesting challenges from an electrical engineering standpoint. Primarily, they block the flow of DC currents and create a non-constant frequency response which can confound experimental results attempting to characterize the electrical characteristics of cells. Due to these, and other, considerations, the use of high-voltage high-frequency signals are necessary to successfully manipulate cells. The effect of these high frequency fields on cells are studied and applied to microfluidic and tissue level applications. In later chapters, theoretical and experimental results show how high frequency and pulsed electric fields can ablate cells and tissue. Understanding the parameters necessary to electroporate cells aids in the development of cDEP devices where this phenomenon is undesirable and gives insight towards the development of new cancer ablation therapies where targeted cell death is sought after. This work shows that there exists a finite frequency spectrum over which cDEP devices can operate in which cells are minimally affected by the induced electric fields. Finally, lessons learned in the course of the development of cDEP were adapted and applied towards cancer ablation therapies where electroporation are favorable. It was found that bursts of high frequency pulsed electric fields can successfully kill cells and ablate tissue volumes through a process termed High Frequency Irreversible Electroporation (H-FIRE). This technique is advantageous as these waveforms mitigate or eliminate muscle contractions associated with traditional IRE technologies. Similarly, the use of fluid electrodes in cDEP inspired the use of an organs vascular system as the conductive pathway to deliver pulses. This novel approach allows for the ablation of large volumes of tissue without the use of puncturing electrodes. These techniques are currently undergoing evaluation in tissue engineering applications and pre-clinical… Advisors/Committee Members: Davalos, Rafael V. (committeechair), Lu, Chang-Tien (committee member), Robertson, John L. (committee member), Rossmeisl, John H. Jr. (committee member), Brown, Gary S. (committee member), Soker, Shay (committee member).

Subjects/Keywords: Irreversible Electroporation; Contactless Dielectrophoresis

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APA (6^th Edition):

Sano, M. B. (2012). Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77122

Chicago Manual of Style (16^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Doctoral Dissertation, Virginia Tech. Accessed February 27, 2021. http://hdl.handle.net/10919/77122.

MLA Handbook (7^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Web. 27 Feb 2021.

Vancouver:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Internet] [Doctoral dissertation]. Virginia Tech; 2012. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10919/77122.

Council of Science Editors:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Doctoral Dissertation]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/77122

The Ohio State University

13. Howdyshell, Marci Lynn. Micro-magnetic Structures for Biological Applications.

Degree: PhD, Physics, 2014, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

► Developments in single-molecule and single-cell experiments over the past century have provided researchers with many tools to probe the responses of cells to stresses such… (more)

Subjects/Keywords: Physics; micro-magnetic traps; electroporation

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APA (6^th Edition):

Howdyshell, M. L. (2014). Micro-magnetic Structures for Biological Applications. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

Chicago Manual of Style (16^th Edition):

Howdyshell, Marci Lynn. “Micro-magnetic Structures for Biological Applications.” 2014. Doctoral Dissertation, The Ohio State University. Accessed February 27, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613.

MLA Handbook (7^th Edition):

Howdyshell, Marci Lynn. “Micro-magnetic Structures for Biological Applications.” 2014. Web. 27 Feb 2021.

Vancouver:

Howdyshell ML. Micro-magnetic Structures for Biological Applications. [Internet] [Doctoral dissertation]. The Ohio State University; 2014. [cited 2021 Feb 27]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613.

Council of Science Editors:

Howdyshell ML. Micro-magnetic Structures for Biological Applications. [Doctoral Dissertation]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

Rutgers University

14. Demiryurek, Yasir, 1985-. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.

Degree: MS, Mechanical and Aerospace Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

► Electroporation-mediated molecular delivery is of interest for many drug-delivery and gene-therapy applications. Recent studies have shown that a two-pulse protocol consisting of a short-duration high-voltage… (more)

▼ Electroporation-mediated molecular delivery is of interest for many drug-delivery and gene-therapy applications. Recent studies have shown that a two-pulse protocol consisting of a short-duration high-voltage first pulse followed by a longer, low-voltage second pulse can increase the efficiency of molecular delivery and preserve more cells alive with suitably chosen pulsing parameters. In this work we investigate the effects of the first and second pulses’ field strength and the inter-pulse delay time on the delivery of two different-sized Fluorescein-Dextran conjugates (10 kDa and 70 kDa). A series of two-pulse electroporation experiments were performed on 3T3 mouse fibroblast cells, with an alternating-current first pulse to permeabilize the cell, followed by a direct-current second pulse to electrophoretically deliver the target molecule into the cell. Our results showed that the delivery amounts of Fluorescein-Dextran varies strongly with the first pulse's field strength and target molecule size. By varying the delay times between two pulses, it is shown that the delivered intracellular concentration of Fluorescein-Dextran decreased linearly with the logarithm of delay time. The data also indicate that membrane resealing after electropermeabilization occurs very rapidly, but that a non-negligible fraction of the pores can be reopened by the second pulse for times on the order of 100 s. The role of the second pulses is seen to be more than just electrophoresis, with a minimum threshold field strength required to reopen nano-sized pores or defects remaining from the first pulse. These results suggest that membrane electroporation, sealing, and reporation is a complex process that has both short-term and long-term components, which may in part explain the wide variation in membrane-resealing times reported in the literature. Advisors/Committee Members: Shan, Jerry (chair), Lin, Hao (internal member), Zahn, Jeffrey (outside member), Shreiber, David (outside member).

Subjects/Keywords: Electroporation; Fluorescein; Gene therapy

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APA (6^th Edition):

Demiryurek, Yasir, 1. (2014). Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

Chicago Manual of Style (16^th Edition):

Demiryurek, Yasir, 1985-. “Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.” 2014. Masters Thesis, Rutgers University. Accessed February 27, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/45233/.

MLA Handbook (7^th Edition):

Demiryurek, Yasir, 1985-. “Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.” 2014. Web. 27 Feb 2021.

Vancouver:

Demiryurek, Yasir 1. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2021 Feb 27]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/.

Council of Science Editors:

Demiryurek, Yasir 1. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

Virginia Tech

15. O'Brien, Timothy J. An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies.

Degree: PhD, Biomedical Engineering, 2019, Virginia Tech

URL: http://hdl.handle.net/10919/101762

► Irreversible electroporation (IRE) is an energy directed focal ablation technique. This procedure typically involves the placement of two or more electrodes into, or around, a… (more)

▼ Irreversible electroporation (IRE) is an energy directed focal ablation technique. This procedure typically involves the placement of two or more electrodes into, or around, a region of interest within the tissue and administering a sequence of short, intense, pulsed electric fields (PEFs). The application of these PEFs results in an increase in the transmembrane potential of all cells within the electric field above a critical value, destabilizing the lipid bilayer of the cellular membrane and increasing the cell-tissue permeability. For years, many have used this phenomenon to assist the transport of macromolecules typically unable to penetrate the cell membrane with the intent of avoiding cell necrosis or irreversible electroporation. More recently, however, irreversible electroporation has proven to be a successful alternative for the treatment of cancer. Proper tuning of the pulse parameters has allowed for a targeted treatment of localized tumors, and has shown immense value in the treatment of surgically inoperable tumors located near major blood vessels and nerves. While it is critical to ensure sufficient treatment of the target tissue, it can be equally vital to the treatment and patients overall outcome that the pulsing conditions are set to moderate the associated thermal effects with the electroporation of biological tissue. The development of thermal mitigation strategies for IRE treatment is the focus of this dissertation. Herein, the underlying theory and thermal considerations of tissue electroporation in various scenarios are described. Additionally, new thermal mitigation approaches with the intention of maintaining tissue temperature below a thermally damaging threshold, while also preserving or improving IRE lesion volume are detailed. Further, numerical models were developed and ex vivo tissue experiments performed using a perfused organ model to examine three thermal mitigation strategies in their ability to moderate temperature. Tests conducted using thermally mitigating treatment delivery on live tissue confirm the capacity to deliver more energy to the tissue at a thermally acceptable temperature, and provide the potential for a replete IRE lesion. Advisors/Committee Members: Davalos, Rafael V. (committeechair), Robertson, John L. (committeechair), Diller, Thomas E. (committee member), McKillop, Iain H. (committee member), Arena, Christopher Brian (committee member).

Subjects/Keywords: Irreversible Electroporation (IRE); High Frequency Irreversible Electroporation (HFIRE); Thermal Mitigation

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APA (6^th Edition):

O'Brien, T. J. (2019). An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/101762

Chicago Manual of Style (16^th Edition):

O'Brien, Timothy J. “An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies.” 2019. Doctoral Dissertation, Virginia Tech. Accessed February 27, 2021. http://hdl.handle.net/10919/101762.

MLA Handbook (7^th Edition):

O'Brien, Timothy J. “An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies.” 2019. Web. 27 Feb 2021.

Vancouver:

O'Brien TJ. An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies. [Internet] [Doctoral dissertation]. Virginia Tech; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10919/101762.

Council of Science Editors:

O'Brien TJ. An Investigation of Thermal Mitigation Strategies for Electroporation-Based Therapies. [Doctoral Dissertation]. Virginia Tech; 2019. Available from: http://hdl.handle.net/10919/101762

Universitat Pompeu Fabra

16. Mercadal Cavaller, Borja. Electroporation and peripheral nerve stimulation.

Degree: Departament de Tecnologies de la Informació i les Comunicacions, 2019, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/667854

► Aquesta tesi té com a objectiu resoldre qüestions en els camps de l’electroporació i l’estimulació dels nervis perifèrics, i sobretot, aquelles que es deriven de… (more)

Subjects/Keywords: Electroporation; Electrical stimulation; Neuromuscular; Irreversible electroporation; Bipolar pulses; High-Frequency irreversible electroporation; Pulsed radiofrequency; Intramuscular stimulation; 62

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APA (6^th Edition):

Mercadal Cavaller, B. (2019). Electroporation and peripheral nerve stimulation. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/667854

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mercadal Cavaller, Borja. “Electroporation and peripheral nerve stimulation.” 2019. Thesis, Universitat Pompeu Fabra. Accessed February 27, 2021. http://hdl.handle.net/10803/667854.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mercadal Cavaller, Borja. “Electroporation and peripheral nerve stimulation.” 2019. Web. 27 Feb 2021.

Vancouver:

Mercadal Cavaller B. Electroporation and peripheral nerve stimulation. [Internet] [Thesis]. Universitat Pompeu Fabra; 2019. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/10803/667854.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mercadal Cavaller B. Electroporation and peripheral nerve stimulation. [Thesis]. Universitat Pompeu Fabra; 2019. Available from: http://hdl.handle.net/10803/667854

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

17. Yuan, Hanwen. Fabrication and characterization of a mcro/nanofluidic platform for electroporation.

Degree: PhD, 2017, University of Louisville

URL: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

► For traditional electroporation devices, there are a number of problems associated with these devices such as insufficient understanding of its theoretical mechanism, low cell… (more)

▼ For traditional electroporation devices, there are a number of problems associated with these devices such as insufficient understanding of its theoretical mechanism, low cell viability, inadequate electroporation efficiency, excess voltage applied to generate required electric field due to the large size of these devices and sample contamination. Although newly developed microfluidic electroporation devices have solved most of the above existing problems in traditional bulk electroporation devices, they appear to lack the ability to control the precise dose of biomolecules or genes transfecting into cells and, from a manufacturing perspective, the fabrication methods do not enable repeatable production of such devices on the large scale. Here, we introduce a new, repeatable method for fabricating 3-D Micro/Nanofluidic electroporation platforms and characterize these platforms to demonstrate their ability to electroporate live cells. Some of the new methods developed in this work include a direct-write fiber technique via three-axis robotic dispensing system, dry film resist photolithography, film-to-film bonding and replica molding to create the desired electroporation platform. A robotic dispensing system was utilized to control the fiber diameter, which was determined vii by the: 1) prescribed dispense time; 2) pressure of the dispensing system valve; 3) rate at which the stage traversed; 4) diameter of the dispensing tip; 5) polymer solution viscosity and surface tension; and, 6) programmed drawing length. Thin dry film photoresist was utilized to replace liquid photoresist in order to achieve high-quality film-to-film bonding after drawing nanofibers onto one substrate containing the thin-film structure. Polydimethylsiloxane (PDMS) was employed as the bulk material to fabricate the target micro/nano electroporation substrate using replica molding and micro/nanofibers etching. Characterization of the direct-write fiber technique via robotic dispensing system to acquire suspended and complex fibers of the required dimension repeatedly under prescribed conditions were completed. Combining this fiber direct-write method and traditional clean room techniques, a total of 18 micro- to nano-scale electroporation devices (6 for each group of 1 ìm, 500 nm, and 300 nm diameter) were successfully developed and mass produced in two weeks with relatively high repeatability (within 20% of the design). Finally, metrology and characterization studies were performed on the electroporation platforms to validate the micro/nanochannel’s existence and its connectivity to two micro-chambers. Furthermore, biomolecules and other fluorescent particles were successfully transported through the micro/nanochannel and transferred (via electroporation) into the cells. Preliminary results of electroporation experiment performed on this micro/nano-electroporation platform illustrated that the duration of the entire electroporation process was significantly shorter than times reported previously by other investigator’s nano-electroporation… Advisors/Committee Members: Keynton, Robert, Williams, Stuart, Brehob, Ellen, Cohn, Robert, Sumanasekera, Gamini, El-Baz, Ayman.

Subjects/Keywords: electroporation; nanochannel; microfluidic; cell; Nanoscience and Nanotechnology

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APA (6^th Edition):

Yuan, H. (2017). Fabrication and characterization of a mcro/nanofluidic platform for electroporation. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

Chicago Manual of Style (16^th Edition):

Yuan, Hanwen. “Fabrication and characterization of a mcro/nanofluidic platform for electroporation.” 2017. Doctoral Dissertation, University of Louisville. Accessed February 27, 2021. 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757.

MLA Handbook (7^th Edition):

Yuan, Hanwen. “Fabrication and characterization of a mcro/nanofluidic platform for electroporation.” 2017. Web. 27 Feb 2021.

Vancouver:

Yuan H. Fabrication and characterization of a mcro/nanofluidic platform for electroporation. [Internet] [Doctoral dissertation]. University of Louisville; 2017. [cited 2021 Feb 27]. Available from: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757.

Council of Science Editors:

Yuan H. Fabrication and characterization of a mcro/nanofluidic platform for electroporation. [Doctoral Dissertation]. University of Louisville; 2017. Available from: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

Penn State University

18. Labarbera, Nicholas Andrew. Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations.

Degree: 2018, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/15917nal5047

► In the past few years, interest has drastically increased in using surgically inserted electrodes to ablate cancer cells. The treatment is referred to as irreversible… (more)

▼ In the past few years, interest has drastically increased in using surgically inserted electrodes to ablate cancer cells. The treatment is referred to as irreversible electroporation (IRE) and has the advantage of being a minimally invasive procedure that can be used to treat tumors while inflicting minimal damage to surrounding tissue and preserving blood vessels. However, treatment planning is required to ensure the electrodes are placed in the correct location and at the proper voltages such that all cancer cells are killed while damaging as few healthy cells as possible. This treatment planning is accomplished through the use of computer simulations. The accuracy of models to predict tissue ablation from IRE is an important component to IRE treatments. It has been well established that the conductivity of tissue increases as the electrical field increases, and a conductivity dependent on electrical field strength is often included in treatment planning models. However, previous work increases conductivity equally in all directions. This dissertation presents a novel formulation that increases the conductivity more in the direction of the electrical field. There is both theoretical and experimental evidence previously published to support this formulation. Results using this novel formulation are compared to previously published models. The second part of the dissertation focuses on performing uncertainty quantification to determine how uncertainty in physical parameters affects the extent of ablation. There is a degree of uncertainty in the material properties of each specific person, as no two humans are identical. There is further uncertainty due to the incomplete knowledge of how the tissue's properties vary during exposure to strong electrical fields. The goal of this research is to provide more knowledge that can be used for the continued development of treatment planning protocols for irreversible electroporation. The third part of the dissertation presents a novel work-flow that will allow medical doctors to perform treatment planning in such a way that they can easily get feedback on how adjustments in electrode number and placement affects possible ablation shapes. The work-flow utilizes linear models to determine possible ablation zones before using nonlinear models to determine voltages necessary to ablate the target zone. Enabling medical doctors to have a more active role in the treatment planning phase when compared to optimization algorithms, should improve the treatment planning process Advisors/Committee Members: Huanyu Cheng, Dissertation Advisor/Co-Advisor, Huanyu Cheng, Committee Chair/Co-Chair, Joseph Paul Cusumano, Committee Member, Corina Stefania Drapaca, Committee Member, Michael Kinzel, Outside Member, Sean McInytre, Special Member.

Subjects/Keywords: Simulation; High Performance Computing; Electroporation; Mathematical Modelling

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APA (6^th Edition):

Labarbera, N. A. (2018). Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/15917nal5047

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Labarbera, Nicholas Andrew. “Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations.” 2018. Thesis, Penn State University. Accessed February 27, 2021. https://submit-etda.libraries.psu.edu/catalog/15917nal5047.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Labarbera, Nicholas Andrew. “Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations.” 2018. Web. 27 Feb 2021.

Vancouver:

Labarbera NA. Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations. [Internet] [Thesis]. Penn State University; 2018. [cited 2021 Feb 27]. Available from: https://submit-etda.libraries.psu.edu/catalog/15917nal5047.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Labarbera NA. Anisotropic Conductivity and Uncertainty Quantification in Irreversible Electroporation Simulations. [Thesis]. Penn State University; 2018. Available from: https://submit-etda.libraries.psu.edu/catalog/15917nal5047

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

19. Zhou, Mingda. Microdevices for Implantation and Neuroscience.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/27475

► Micro-electro-mechanical system (MEMS) and the microfabrication techniques, whose feature size covers a range of several microns to hundreds of microns, provides us the potential to… (more)

▼ Micro-electro-mechanical system (MEMS) and the microfabrication techniques, whose feature size covers a range of several microns to hundreds of microns, provides us the potential to develop miniaturized sensor and actuator for implantation applications and an unique way to address the biological and biomedical issues in microscopic and mesoscopic level. Three different devices were developed with MEMS techniques. One device is an implantable MEMS pressure sensor prototype that can be seamlessly integrated with continuous flow left ventricular assist device (LVAD) to enable real-time control, optimize its performance and reduce its risks. The pressure sensing mechanism is based on Fabry-Pérot interferometer principle. The sensitivity, range and response time of the pressure sensor are measured and validated to meet the requirements of LVAD pressure sensing. A second device is a novel MEMS chopper modulator (MCM) that enables the chopper modulation in gas chromatography electroantennography (GC-EAG) by modulating the effluent from the GC separation column before it reaches the insect antenna. The key component of MCM is a MEMS flow control chip, fabricated with fully GC compatible materials using microfabrication techniques. It can reside inside the conventional GC oven, switch GC flow with on/off ratio as high as 1000:1, and operate reliably for more than 1 million switching cycles at working temperature of 300 °C. Using the new MEMS chopper modulated GC-EAG (MCM-GC-EAG), we demonstrated as little as 1 pg pheromone cis-11-hexadecenal could be successfully detected using the antenna harvested from male moth Helicoverpa Virescens. A third device is a needle electroporator, which is an integrated microdevice that can perform high spatial resolution electroporation, targeting several neurons to tens of neurons, in deep brain. In vitro electroporation on MDA-MB-231 cells was performed with the device and cell membrane non-permeable dyes to investigate the effect of electric impulse (pulse amplitude, pulse duration, pulse interval and the number of pulses) on the success of electroporation and the viability of cells, and to determine the optimal condition. In vitro electroporation with was performed on HEK-293T cells using the device and plasmid that encodes green fluorescent protein (GFP) gene to confirm that the optimal condition determined with fluorescent dyes also works for plasmid. In vivo electroporation with a plasmid that encodes GFP as a reporter gene was successfully demonstrated at hippocampus of mouse brain. Advisors/Committee Members: Siyang Zheng, Dissertation Advisor/Co-Advisor, Siyang Zheng, Committee Chair/Co-Chair, William O Hancock, Committee Member, Yingwei Mao, Committee Member, Nanyin Zhang, Committee Member.

Subjects/Keywords: MEMS; Implantation; gas chromatography electroantennography; Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhou, M. (2015). Microdevices for Implantation and Neuroscience. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhou, Mingda. “Microdevices for Implantation and Neuroscience.” 2015. Thesis, Penn State University. Accessed February 27, 2021. https://submit-etda.libraries.psu.edu/catalog/27475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhou, Mingda. “Microdevices for Implantation and Neuroscience.” 2015. Web. 27 Feb 2021.

Vancouver:

Zhou M. Microdevices for Implantation and Neuroscience. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Feb 27]. Available from: https://submit-etda.libraries.psu.edu/catalog/27475.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhou M. Microdevices for Implantation and Neuroscience. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27475

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

North Carolina State University

20. Becker, Sid. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.

Degree: PhD, Mechanical Engineering, 2007, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/4991

► Electroporation is an approach used to enhance the transport of large molecules to the cell cytosol in which a targeted tissue region is exposed to… (more)

Subjects/Keywords: skin; electroporation; numerical

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Becker, S. (2007). In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/4991

Chicago Manual of Style (16^th Edition):

Becker, Sid. “In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.” 2007. Doctoral Dissertation, North Carolina State University. Accessed February 27, 2021. http://www.lib.ncsu.edu/resolver/1840.16/4991.

MLA Handbook (7^th Edition):

Becker, Sid. “In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.” 2007. Web. 27 Feb 2021.

Vancouver:

Becker S. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. [Internet] [Doctoral dissertation]. North Carolina State University; 2007. [cited 2021 Feb 27]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/4991.

Council of Science Editors:

Becker S. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. [Doctoral Dissertation]. North Carolina State University; 2007. Available from: http://www.lib.ncsu.edu/resolver/1840.16/4991

Brigham Young University

21. Wilson, Aubrey Marie Mueller. Transgene Delivery via Microelectromechanical Systems.

Degree: MS, 2012, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

► The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene… (more)

▼ The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene delivery option. In this work we address the current progress of microelectromechanical systems (MEMS) used as transgenic delivery mechanisms. The nanoinjector is a specially designed MEMS device which uses electrostatic charge to manipulate transgene molecules. The process of nanoinjection was designed as an alternative to microinjection which causes less damage to developing embryos, improves embryo survival, birth rates, and overall efficiency of injections. In vivo testing of nanoinjection demonstrates it is both safe and effective. Additionally nanoinjection has the potential to make transgenesis via yeast artificial chromosomes more practical as the nanoinjector may prevent shearing of the YAC molecules. A second nanoinjection protocol termed intracellular electroporetic nanoinjcetion (IEN) was designed to allow for cytoplasmic injections. Cytoplasmic injections are faster and easier than pronuclear injection and do not require the pronuclei to be visible; yet previous attempts to develop cytoplasmic injection have met with limited success. In IEN injections the nanoinjector is used to place transgenic molecules in the cytoplasm. The transgenes are then propelled through the cytoplasm and electroporated into the pronucleus using electrical pulses. Electroporation of whole embryos has not resulted in transgenic animals, but the MEMS device allows localized electroporation to occur within the cytoplasm, giving transgene access to the pronucleus before degradation can occur. In this report we describe the principles which allow for localized electroporation of the pronuclei including: the location of the pronuclei between 21-28 hours post-hCG treatment, modeling data predicting the voltages needed for localized electroporation of pronuclei, and data on the movement of transgenic DNA based on the voltages delivered by IEN. We further report results of an IEN versus microinjection comparative study in which IEN produced transgenic pups with viability, transgene integration, and expression rates statistically comparable to microinjection. The ability to perform injections without visualizing or puncturing the pronuclei will widely benefit transgenic research, and will be particularly advantageous for the production of transgenic animals with embryos exhibiting reduced pronuclear visibility.

Subjects/Keywords: nanoinjection; microinjection; transgenic; electroporation; DNA transfer; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wilson, A. M. M. (2012). Transgene Delivery via Microelectromechanical Systems. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

Chicago Manual of Style (16^th Edition):

Wilson, Aubrey Marie Mueller. “Transgene Delivery via Microelectromechanical Systems.” 2012. Masters Thesis, Brigham Young University. Accessed February 27, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd.

MLA Handbook (7^th Edition):

Wilson, Aubrey Marie Mueller. “Transgene Delivery via Microelectromechanical Systems.” 2012. Web. 27 Feb 2021.

Vancouver:

Wilson AMM. Transgene Delivery via Microelectromechanical Systems. [Internet] [Masters thesis]. Brigham Young University; 2012. [cited 2021 Feb 27]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd.

Council of Science Editors:

Wilson AMM. Transgene Delivery via Microelectromechanical Systems. [Masters Thesis]. Brigham Young University; 2012. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

22. Sghari, Soufien. Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2015, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2015SACLS053

►

La crête neurale céphalique (CNC) est une structure pluripotente à l'origine de la totalité du squelette de la face et de la voûte crânienne. L'absence de… (more)

▼

La crête neurale céphalique (CNC) est une structure pluripotente à l'origine de la totalité du squelette de la face et de la voûte crânienne. L'absence de la CNC est associée à des malformations du cerveau antérieur et des défauts oculaires qui reproduisent les malformations congénitales humaines: microcéphalie, holoprosencéphalie, aniridie, chambre antérieure manquante, colobome congénital, désorganisation du cristallin, agénésie des paupières, strabisme précoce (Alward 2000, Creuzet 2009, Williams and Bohnsack 2015). Dans notre étude, l'augmentation hétérochronique de la signalisation Bmp7 au niveau de la plaque préchordale (PPC) a engendré une perturbation de la migration de la CNC associée à une fusion des deux champs oculaires en un seul œil cyclope. Cette cyclopie, contrairement aux études antérieures (Chiang et al. 1996, Golden et al. 1999) est précédée par une perturbation de la constriction apicale au niveau des vésicules optiques (VO) au stade Hamburger-Hamilton (HH) 12 et qui est responsable de l'invagination dorsale des VO et leur séparation du reste du tube neural. Au niveau moléculaire, nous avons enregistré une augmentation de la signalisation Wnt1 très tot après le traitement (2h) dans la CNC. Cette augmentation s'étend rostralement ce qui suggère que Wnt1 pourrait etre impliqué dans la perturbation du développement oculaire. Pour tester cette hypothèse, nous avons tenté des expériences de sauvetage par l'inactivation de la signalisation Wnt1 par ARN interférence. Nous avons observé une amélioration du phénotype mais les yeux sont restés morphologiquement en hypotélorisme, c'est-à-dire trop proches. Des coupes coronales au stade E8 (32-33HH) ont montré que les yeux sont synophtalmiques, deux yeux dans un seul orbite, et sont restés fusionnés au télencéphale. Au niveau moléculaire, nous avons observé aussi une diminution de l'expression de Wnt2b au niveau des VO chez l'embryon cyclope et que cette diminution persiste malgré le sauvetage par Wnt1. Pour comprendre cette signalisation, nous avons tenté une deuxième expérience de sauvetage avec ARN interférence contre Wnt1 et un vecteur plasmidique exprimant Wnt2b dans le tube neural et les VO respectivement. Vers E8 l'embryon ressemble au contrôle mais avec persistance de fusion entre les VO et le télencéphale. L'hybridation in situ a montré une diminution du niveau d'expression de Foxg1 à 12HH dans la partie dorsale des VO au niveau de la frontière entre la partie rostrale du diencéphale et le télencéphale (RD/T) dans les deux tentatives de sauvetage ce qui suggère que ce gène pourrait être impliqué dans la séparation rostrale des VO du télencéphale.

Cephalic neural crest (CNC) is a pluripotent structure giving rise to entire skeleton of the face and skull. Absence of CNC is associated with forebrain and eye defects that mimic human congénital malformations: microcephaly, holoprosencephaly, aniridia, missing anterior chamber, coloboma, congenital dislocation of the lens, agenesis of eyelid and early strabismus. (Alward 2000 Creuzet 2009, Williams and…

Advisors/Committee Members: Creuzet, Sophie (thesis director).

Subjects/Keywords: Cnc; Cyclopie; Poulet; Cnc; Cyclopia; Chicken; Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sghari, S. (2015). Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2015SACLS053

Chicago Manual of Style (16^th Edition):

Sghari, Soufien. “Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye.” 2015. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed February 27, 2021. http://www.theses.fr/2015SACLS053.

MLA Handbook (7^th Edition):

Sghari, Soufien. “Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye.” 2015. Web. 27 Feb 2021.

Vancouver:

Sghari S. Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2015. [cited 2021 Feb 27]. Available from: http://www.theses.fr/2015SACLS053.

Council of Science Editors:

Sghari S. Rôle morphogénétique de la crête neurale céphalique au cours du développement précoce de l'oeil : Morphogenetic role of cephalic neural crest during early development of the eye. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2015. Available from: http://www.theses.fr/2015SACLS053

Vytautas Magnus University

23. Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.

Degree: Master, Biology, 2008, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

►

Šiame darbe buvo atliekami elektrinio DNR perkėlimo mechanizmų tyrinėjimai in vitro naudojant dviejų tipų impulsus –aukštos įtampos trumpus impulsus (HV) ir žemos įtampos ilgus impulsus… (more)

▼

Šiame darbe buvo atliekami elektrinio DNR perkėlimo mechanizmų tyrinėjimai in vitro naudojant dviejų tipų impulsus –aukštos įtampos trumpus impulsus (HV) ir žemos įtampos ilgus impulsus (LV). DNR elektropernašos eksperimentai buvo atlikti in vitro su Kinijos žiurkėno kiaušidžių CHO ląstelėmis. Eksperimentais buvo siekiama įvertinti HV ir LV impulsų svarbą DNR elektropernašos efektyvumui. Tyrimai parodė, kad DNR elektropernaša vyksta tik po ląstelių elektroporacijos. Naudojant tik LV impulsą (100 V/cm impulso stipris, 100 ms impulso trukmė), kuris ląstelių neelektroporavo, DNR elektropernaša nevyko. LV impulso svarbą DNR elektrotransfekcijos efektyvume, mes tyrėme taikydami LV impulsus 1s po HV impulsų. Kitų autorių in vivo tyrimai parodė, kad jei LV impulsas buvo taikomas po HV impulso, LV smarkiai padidino tranfekcijos efektyvumą. Priešingai nei in vivo eksperimentuose, mūsų in vitro eksperimentai tokios LV impulsų svarbos transfekcijos efektyvumui neparodė. Iš tikrųjų, nei LV impulso amplitudės padidėjimas, nei LV impulsų skaičiaus padidėjimas nepadidino DNR elektrotransfekcijos efektyvumo. Nors, naudojant įvairias HV ir LV impulsų kombinacijas elektrotransfekcijos efektyvumas nebuvo pastovus, jis nesiskyrė žymiai nuo efektyvumo, pasiekto tiktai su HV impulsu.

In this study we setup our experiments to evaluate the roles of high-voltage, short-duration (HV) and low-voltage, long duration (LV) pulses for efficient DNA electrotransfer into cells in vitro. Consistently with others and our previous in vitro and in vivo results, this study confirmed that DNA electrotransfer is possible only if cell permeabilization is achieved. Cell treatment with only one LV pulse (100 V/cm pulse strength, 100 ms pulse duration), that do not permeabilize cells had no effect for DNA electrotransfer. Contribution of LV pulse on overall DNA electrotransfer efficacy we tested by applying LV pulse 1 s following HV pulse. In previous in vivo study such type of experiments revealed that once cell is electropermeabilized, DNA electrotransfer efficacy is governed by the parameters of LV pulse. To further characterize how LV pulses facilitate DNA electrotransfer across the permeabilized membrane we setup this in vitro study. Contrary to in vivo studies, this work revealed that contribution of the LV pulses is negligible. Indeed, neither increase in LV pulse number, nor increase in LV pulse amplitude increased efficacy of DNA electrotransfer. Although, various combinations of HV and LV pulses resulted in slightly variable electrotransfer efficacy, it did not differ significantly from the efficacy achieved with only HV pulse.

Advisors/Committee Members: Šatkauskas, Saulius (Master’s thesis supervisor), Saulis, Gintautas (Master’s thesis reviewer).

Subjects/Keywords: Elektroporacija; Elektrogenoterapija; Elektropernaša; Electroporation; Electrogeneterapy; Electrotransfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kačkauskaitė, Dovilė. (2008). Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. (Masters Thesis). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Kačkauskaitė, Dovilė. “Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.” 2008. Masters Thesis, Vytautas Magnus University. Accessed February 27, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Kačkauskaitė, Dovilė. “Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.” 2008. Web. 27 Feb 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. [Internet] [Masters thesis]. Vytautas Magnus University; 2008. [cited 2021 Feb 27]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. [Masters Thesis]. Vytautas Magnus University; 2008. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vytautas Magnus University

24. Čepurnienė, Karolina. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.

Degree: PhD, Biochemistry, 2010, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

►

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency… (more)

▼

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency into the cells both in suspension and in monolayer. In order to deliver various combinations of HV and LV electric pulses an original electroporator has been developed. Contrary to many studies in vivo and several studies in vitro we have shown that LV pulses do not increase number of transfected cells both in suspension and in monolayer, when 100 µg/ml plasmid concentration was used. Analyzing DNA electrotransfer into CHO cells grown in monolayer we observed, that some of these cell fuse forming multinuclear cell structures. Examining this effect we have shown that cell fusion depends on parameters of electric pulses and more intriguingly on cells type. We have shown that the extent of electrofusion in B16F10 cells was significantly larger than other types of cells. Thus these results allowed us to hypothesize that cells from metastatic tumours have greater propensity for cell electrofusion. This effect has been investigated in more detail. Using GFP and luciferase coding plasmids at various concentrations (1, 10 and 100 µg/ml) we were the first to demonstrate that the increase in transfection efficiency by LV pulses is evident only at reduced plasmid concentration. This increase, in comparison to transfection induced using HV pulse alone, was more pronounced with more reduced concentrations of the plasmid.... [to full text]

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Šių tyrimų rezultatai parodė, kad mažų molekulių, tokių kaip propidžio jodidas ir didelių molekulių, tokių, kaip plazmidinė DNR, elektropernašos į ląsteles in vitro mechanizmai yra skirtingi. Skirtingai nuo mažų molekulių patekimo, plazmidinės DNR elektropernaša galima tik tuo atveju jei plazmidė ląstelių suspensijoje yra AA impulso taikymo metu. Naudodami AA ir ŽA impulsų kombinacijas, savo tyrimais pirmieji parodėme, kad suspensijoje ir ląstelėms augančioms monosluoksniu, ŽA impulsai po ląstelių elektroporacijos AA impulsu, kai naudota 100 µg/ml plazmidės koncentracija, transfekuotų ląstelių skaičiui įtakos neturi. Tiriant molekulių patekimą į ląsteles paveikus jas elektriniais laukais pastebėjome, kad kai kurios ląstelės augančios monosluoksniu gali susilieti. Tyrimais nustatėme, kad ląstelių susiliejimas priklauso nuo elektrinio lauko stiprio ir ląstelių linijos. Mūsų in vitro tyrimų rezultatai parodė kad daugiausia ląstelių po elektroporacijos susilieja B16F10 linijoje ląstelėse. Tai patvirtino ir sustiprino ankstesnes hipotezes, kad iš metastazuojančių navikų, ląstelių elektrosusiliejimo kiekis žymiai didesnis, nei normalių ląstelių. Vertinant ŽA impulso įtaką perneštos DNR kiekiui, nustatėme, kad ŽA impulso įtaka stebima kuomet ląstelių suspensijoje plazmidės koncentracija yra sumažinta (10 µg/ml, 1 µg/ml).... [toliau žr. visą tekstą]

Advisors/Committee Members: Šatkauskas, Saulius (Doctoral dissertation supervisor), Skeberdis, Vytenis Arvydas (Doctoral dissertation opponent), Kirvelienė, Vida (Doctoral dissertation opponent), Paulauskas, Algimantas (Doctoral dissertation committee chair), Mildažienė, Vida (Doctoral dissertation committee member), Rukšėnas, Osvaldas (Doctoral dissertation committee member), Meškys, Rolandas (Doctoral dissertation committee member), Jurevičius, Jonas (Doctoral dissertation committee member).

Subjects/Keywords: Electroporation; Electrotransfection; DNA; Electroporacija; Elektrotransfekcija; DNR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Čepurnienė, K. (2010). Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. (Doctoral Dissertation). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

Chicago Manual of Style (16^th Edition):

Čepurnienė, Karolina. “Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.” 2010. Doctoral Dissertation, Vytautas Magnus University. Accessed February 27, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;.

MLA Handbook (7^th Edition):

Čepurnienė, Karolina. “Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.” 2010. Web. 27 Feb 2021.

Vancouver:

Čepurnienė K. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. [Internet] [Doctoral dissertation]. Vytautas Magnus University; 2010. [cited 2021 Feb 27]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;.

Council of Science Editors:

Čepurnienė K. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. [Doctoral Dissertation]. Vytautas Magnus University; 2010. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

Vytautas Magnus University

25. Čepurnienė, Karolina. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.

Degree: Dissertation, Biochemistry, 2010, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

►

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Tyrimų rezultatai parodė, kad… (more)

▼

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Tyrimų rezultatai parodė, kad mažų molekulių, tokių kaip propidžio jodidas ir didelių molekulių, tokių, kaip plazmidinė DNR, elektropernašos į ląsteles in vitro mechanizmai yra skirtingi. Skirtingai nuo mažų molekulių patekimo, plazmidinės DNR elektropernaša galima tik tuo atveju jei plazmidė ląstelių suspensijoje yra AA impulso taikymo metu. Naudodami AA ir ŽA impulsų kombinacijas, savo tyrimais pirmieji parodėme, kad suspensijoje ir ląstelėms augančioms monosluoksniu, ŽA impulsai po ląstelių elektroporacijos AA impulsu, kai naudota 100 µg/ml plazmidės koncentracija, transfekuotų ląstelių skaičiui įtakos neturi. Tiriant molekulių patekimą į ląsteles paveikus jas elektriniais laukais pastebėjome, kad kai kurios ląstelės augančios monosluoksniu gali susilieti. Tyrimais nustatėme, kad ląstelių susiliejimas priklauso nuo elektrinio lauko stiprio ir ląstelių linijos. Mūsų in vitro tyrimų rezultatai parodė kad daugiausia ląstelių po elektroporacijos susilieja B16F10 linijoje ląstelėse. Tai patvirtino ir sustiprino ankstesnes hipotezes, kad iš metastazuojančių navikų, ląstelių elektrosusiliejimo kiekis žymiai didesnis, nei normalių ląstelių. Vertinant ŽA impulso įtaką perneštos DNR kiekiui, nustatėme, kad ŽA impulso įtaka stebima kuomet ląstelių suspensijoje plazmidės koncentracija yra sumažinta (10 µg/ml, 1 µg/ml).... [toliau žr. visą tekstą]

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency into the cells both in suspension and in monolayer. In order to deliver various combinations of HV and LV electric pulses an original electroporator has been developed. Contrary to many studies in vivo and several studies in vitro we have shown that LV pulses do not increase number of transfected cells both in suspension and in monolayer, when 100 µg/ml plasmid concentration was used. Analyzing DNA electrotransfer into CHO cells grown in monolayer we observed, that some of these cell fuse forming multinuclear cell structures. Examining this effect we have shown that cell fusion depends on parameters of electric pulses and more intriguingly on cells type. We have shown that the extent of electrofusion in B16F10 cells was significantly larger than other types of cells. Thus these results allowed us to hypothesize that cells from metastatic tumours have greater propensity for cell electrofusion. This effect has been investigated in more detail. Using GFP and luciferase coding plasmids at various concentrations (1, 10 and 100 µg/ml) we were the first to demonstrate that the increase in transfection efficiency by LV pulses is evident only at reduced plasmid concentration. This increase, in comparison to transfection induced using HV pulse alone, was more pronounced with more reduced concentrations of the plasmid.... [to full text]

Advisors/Committee Members: Šatkauskas, Saulius (Doctoral dissertation supervisor), Skeberdis, Vytenis Arvydas (Doctoral dissertation opponent), Kirvelienė, Vida (Doctoral dissertation opponent), Paulauskas, Algimantas (Doctoral dissertation committee chair), Mildažienė, Vida (Doctoral dissertation committee member), Rukšėnas, Osvaldas (Doctoral dissertation committee member), Meškys, Rolandas (Doctoral dissertation committee member), Jurevičius, Jonas (Doctoral dissertation committee member).

Subjects/Keywords: Elektroporacija; Elektrotransfekcija; DNR; Electroporation; Electrotransfection; DNA

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Čepurnienė, K. (2010). DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. (Doctoral Dissertation). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

Chicago Manual of Style (16^th Edition):

Čepurnienė, Karolina. “DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.” 2010. Doctoral Dissertation, Vytautas Magnus University. Accessed February 27, 2021. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;.

MLA Handbook (7^th Edition):

Čepurnienė, Karolina. “DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.” 2010. Web. 27 Feb 2021.

Vancouver:

Čepurnienė K. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. [Internet] [Doctoral dissertation]. Vytautas Magnus University; 2010. [cited 2021 Feb 27]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;.

Council of Science Editors:

Čepurnienė K. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. [Doctoral Dissertation]. Vytautas Magnus University; 2010. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

26. Frazier, Hilaree Noele. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.

Degree: MS, Biological Sciences, 2016, Encompass Digital Archive, Eastern Kentucky University

URL: https://encompass.eku.edu/etd/365

► The process of using electroporation to introduce plasmid DNA into host cells is a valuable molecular technique that is increasingly employed in labs worldwide.… (more)

Subjects/Keywords: bacteria; electroporation; listeria; plasmid; Microbiology; Molecular Biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frazier, H. N. (2016). Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. (Masters Thesis). Encompass Digital Archive, Eastern Kentucky University. Retrieved from https://encompass.eku.edu/etd/365

Chicago Manual of Style (16^th Edition):

Frazier, Hilaree Noele. “Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.” 2016. Masters Thesis, Encompass Digital Archive, Eastern Kentucky University. Accessed February 27, 2021. https://encompass.eku.edu/etd/365.

MLA Handbook (7^th Edition):

Frazier, Hilaree Noele. “Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.” 2016. Web. 27 Feb 2021.

Vancouver:

Frazier HN. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. [Internet] [Masters thesis]. Encompass Digital Archive, Eastern Kentucky University; 2016. [cited 2021 Feb 27]. Available from: https://encompass.eku.edu/etd/365.

Council of Science Editors:

Frazier HN. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. [Masters Thesis]. Encompass Digital Archive, Eastern Kentucky University; 2016. Available from: https://encompass.eku.edu/etd/365

University of Manitoba

27. Salimi, Elham. Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation.

Degree: Electrical and Computer Engineering, 2011, University of Manitoba

URL: http://hdl.handle.net/1993/4460

► Nanosecond pulse electroporation of biological cells is gaining significant interest due to its ability to influence intracellular structures. In nanosecond pulse electroporation of biological cells… (more)

Subjects/Keywords: Electroporation; Dielectric dispersion; Debye relaxation model

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Salimi, E. (2011). Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/4460

Chicago Manual of Style (16^th Edition):

Salimi, Elham. “Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation.” 2011. Masters Thesis, University of Manitoba. Accessed February 27, 2021. http://hdl.handle.net/1993/4460.

MLA Handbook (7^th Edition):

Salimi, Elham. “Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation.” 2011. Web. 27 Feb 2021.

Vancouver:

Salimi E. Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation. [Internet] [Masters thesis]. University of Manitoba; 2011. [cited 2021 Feb 27]. Available from: http://hdl.handle.net/1993/4460.

Council of Science Editors:

Salimi E. Nanosecond pulse electroporation of biological cells: The effect of membrane dielectric relaxation. [Masters Thesis]. University of Manitoba; 2011. Available from: http://hdl.handle.net/1993/4460

Hong Kong University of Science and Technology

28. Gao, Wenhui. Study of DNA-uptake mechanisms in electroporation.

Degree: 1998, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

► Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by… (more)

▼ Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by cells, however, have not yet been clearly understood. In this thesis, we have summarized a series of experiment designed to examine the DAN- uptake mechanisms. In addition, cell treatments that can enhance the efficiency of electroporation are also discussed. The results show that after the application of electric field the additional treatment by using a recovery medium containing proper amounts of divalent ions can significantly increase the transfection efficiency in 3T6, Pt K2 and SK-N-SH cells. Several factors, including the strength of electric field, recovery time and concentrations of Ca++ or Mg++ were examined. The reason for increasing the transfection efficiency is believed to be mainly caused by promoting the entry of DNA through the cell membrane, rather than by enhancing the expression of the transferred gene. It has been suggested that DNA-uptake is a multiple- step process. (1) DNA may be driven into the cell by an electrophoretic force when the membrane pores were opened during the application of the electric field. (2) After the electric field was turned off, DNA may continue to diffuse into the cell before the membrane pores were closed. (3) Finally, DNA may be carried in the cell by endocytosis after the membrane pores were resealed. In this research, we have designed specific experiments to test each of these steps. Our results support steps (1) and (3), but not step (2). We found that the kinetics of membrane pore resealing depends strongly on the size of the transport molecules. Thus, uptake of large molecules such as FITC labeled dextran (70 kD) or DNA stopped almost within seconds after the electric field was turned off. In summary, we propose that the DNA-uptake mechanisms in electroporation involve a fast step of DNA entrance driven by the electric field, and a relatively slower process of DNA-uptake by endocytosis following the turn-off of the electric pulses. The experimental evidence will be discussed in detail in this thesis.

Subjects/Keywords: Electroporation ; DNA ; Transfection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, W. (1998). Study of DNA-uptake mechanisms in electroporation. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Thesis, Hong Kong University of Science and Technology. Accessed February 27, 2021. http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Web. 27 Feb 2021.

Vancouver:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Internet] [Thesis]. Hong Kong University of Science and Technology; 1998. [cited 2021 Feb 27]. Available from: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Thesis]. Hong Kong University of Science and Technology; 1998. Available from: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

29. Yang, Fan. Fabrication and characterization of a micro electroporation cell chip.

Degree: 2004, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-5264 ; https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

► Electroporation is one of the most important gene transfer methods in biotechnology, which employs electrical pulse applied across a cell for cell membrane permeabilization. Compared… (more)

Subjects/Keywords: Electroporation ; Cytology – Technique

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, F. (2004). Fabrication and characterization of a micro electroporation cell chip. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-5264 ; https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Fan. “Fabrication and characterization of a micro electroporation cell chip.” 2004. Thesis, Hong Kong University of Science and Technology. Accessed February 27, 2021. http://repository.ust.hk/ir/Record/1783.1-5264 ; https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Fan. “Fabrication and characterization of a micro electroporation cell chip.” 2004. Web. 27 Feb 2021.

Vancouver:

Yang F. Fabrication and characterization of a micro electroporation cell chip. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2004. [cited 2021 Feb 27]. Available from: http://repository.ust.hk/ir/Record/1783.1-5264 ; https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang F. Fabrication and characterization of a micro electroporation cell chip. [Thesis]. Hong Kong University of Science and Technology; 2004. Available from: http://repository.ust.hk/ir/Record/1783.1-5264 ; https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Notre Dame

30. Ashley Nicole Swanson. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.

Degree: Biological Sciences, 2016, University of Notre Dame

URL: https://curate.nd.edu/show/ff365427t4d

► Mycobacterium avium is an environmental bacteria that can cause disease in people with pre-existing lung conditions or who are immunocompromised. Mycobacteria are well known… (more)

Subjects/Keywords: Glycopeptidolipids; RNA Sequencing; Mycobacterium avium; Transformation; Electroporation

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Swanson, A. N. (2016). Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/ff365427t4d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Swanson, Ashley Nicole. “Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.” 2016. Thesis, University of Notre Dame. Accessed February 27, 2021. https://curate.nd.edu/show/ff365427t4d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Swanson, Ashley Nicole. “Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.” 2016. Web. 27 Feb 2021.

Vancouver:

Swanson AN. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. [Internet] [Thesis]. University of Notre Dame; 2016. [cited 2021 Feb 27]. Available from: https://curate.nd.edu/show/ff365427t4d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Swanson AN. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. [Thesis]. University of Notre Dame; 2016. Available from: https://curate.nd.edu/show/ff365427t4d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations