subject:(electroporation)

Rutgers University

1. Yu, Miao, 1984-. A model study of molecular transport in electroporation.

Degree: PhD, Mechanical and Aerospace Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

►

Reversible electroporation is a non-viral technique to introduce foreign molecules into biological cells or tissues, which has found applications in fields including gene transfer, cancer… (more)

▼

Reversible electroporation is a non-viral technique to introduce foreign molecules into biological cells or tissues, which has found applications in fields including gene transfer, cancer treatment, stem-cell research etc. Despite its promising potential, the improvement of electroporation technique is impeded by the lack of a comprehensive understanding of the underlying mechanisms involved in the process of molecular delivery. This work aims at implementing model studies of electroporation-mediated molecular delivery with the target varying from small molecules (propidium iodide, PI) to macromolecules (DNA). Three significant tasks have been accomplished. First, a model study is performed on the electroporation-mediated delivery of PI. In particular, the effects of extra-cellular conductivity on the amount of PI delivery are carefully investigated and discussed. The results are extensively compared with experiments by Sadik et al., and reveal important physical insights about the transport mechanisms involved. It is confirmed that the electrophoretic transport, not the diffusive transport, is the dominating mechanism in mediating PI delivery, and the inverse correlation observed between PI delivery and extra-cellular conductivity results from an electrokinetic phenomenon termed Field Amplified Sample Stacking (FASS). Second, a model investigation of Fluorescein-Dextran delivery is implemented for double-pulse electroporation. Simulated results find qualitative agreement with experiments in predicting the correlation between delivery and pulsing parameters. A bifurcation analysis of equilibrium pore size with respect to the transmembrane potential is presented to explain the observed “critical field strength” above which the second pulse abruptly becomes effective in mediating delivery. Third, a 1D Fokker-Planck simulation is used to characterize the process of DNA translocation through an electropore under finite DC pulses. It is found that the translocation may occur on two disparate time scales, the electrophoretic time (~ ms), and the diffusive time (~ s), depending on the pulse length. Furthermore, a power-law correlation is observed between the final probability of successful translocation and pulsing parameters. Simulated results are compared with previous data to interpret the trends, and further model predictions are made which can be verified by well-designed experiments. Together, these projects establish connections between available theoretical model and experimental observations in electroporation research. Such a connection on one hand benefits experimentalists in providing a powerful prediction tool for the design and optimization of electroporation; on the other hand it equally benefits theorists to improve the models and advance fundamental understandings in the subject.

Advisors/Committee Members: Lin, Hao (chair), Shan, Jerry (internal member), Bagchi, Prosenjit (internal member), Shreiber, David (outside member).

Subjects/Keywords: Electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yu, Miao, 1. (2014). A model study of molecular transport in electroporation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

Chicago Manual of Style (16^th Edition):

Yu, Miao, 1984-. “A model study of molecular transport in electroporation.” 2014. Doctoral Dissertation, Rutgers University. Accessed September 15, 2019. https://rucore.libraries.rutgers.edu/rutgers-lib/45583/.

MLA Handbook (7^th Edition):

Yu, Miao, 1984-. “A model study of molecular transport in electroporation.” 2014. Web. 15 Sep 2019.

Vancouver:

Yu, Miao 1. A model study of molecular transport in electroporation. [Internet] [Doctoral dissertation]. Rutgers University; 2014. [cited 2019 Sep 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/.

Council of Science Editors:

Yu, Miao 1. A model study of molecular transport in electroporation. [Doctoral Dissertation]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45583/

Rutgers University

2. Zheng, Mingde, 1987-. Development of a continuous-flow, automated microfluidic device for single cell level electroporation.

Degree: PhD, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

►

Electroporation is a powerful transfection technique that creates transient openings in the cell membrane by applying an electric field, allowing for the intracellular delivery of… (more)

▼

Electroporation is a powerful transfection technique that creates transient openings in the cell membrane by applying an electric field, allowing for the intracellular delivery of diagnostic and therapeutic substances. The ability to detect and control the degree of cell membrane permeability plays a key role in determining the size of the delivery payload, while safeguarding the overall cell viability. In order to create a universal electroporation system, this dissertation describes the development of a continuous flow electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. In contrast to devices that immobilize individual cells for impedance analysis, this work demonstrates the capability to manipulate single cells under flow and real-time analysis of membrane permeabilization before and after electroporation, which dramatically increasing the number of cells which can be electroporated and analyzed. Using an electric circuit model, and Multiphysics computational tools, the key parameters for successful cell membrane permeabilization detection in a flow environment were determined. By varying the electric field parameters, we demonstrate the direct control of cell membrane permeabilization by electrically measuring the electroporation-induced cell membrane impedance change and by optically measuring the delivery of a fluorescent probe. Viability of the electroporated cells following collection also demonstrates a correlation with the applied pulse strength. By extending the device capability to include dynamic pulse adjustment according to the real-time feedback information on cell viability, an intelligent electroporation system capable of potentially maximizing delivery efficiency and cell viability can be thus realized.

Advisors/Committee Members: Zahn, Jeffrey D. (chair), Shreiber, David I. (internal member), Tischfield, Jay A. (internal member), Lin, Hao (outside member), Shan, Jerry W. (outside member).

Subjects/Keywords: Electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zheng, Mingde, 1. (2016). Development of a continuous-flow, automated microfluidic device for single cell level electroporation. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

Chicago Manual of Style (16^th Edition):

Zheng, Mingde, 1987-. “Development of a continuous-flow, automated microfluidic device for single cell level electroporation.” 2016. Doctoral Dissertation, Rutgers University. Accessed September 15, 2019. https://rucore.libraries.rutgers.edu/rutgers-lib/51538/.

MLA Handbook (7^th Edition):

Zheng, Mingde, 1987-. “Development of a continuous-flow, automated microfluidic device for single cell level electroporation.” 2016. Web. 15 Sep 2019.

Vancouver:

Zheng, Mingde 1. Development of a continuous-flow, automated microfluidic device for single cell level electroporation. [Internet] [Doctoral dissertation]. Rutgers University; 2016. [cited 2019 Sep 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/.

Council of Science Editors:

Zheng, Mingde 1. Development of a continuous-flow, automated microfluidic device for single cell level electroporation. [Doctoral Dissertation]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51538/

Hong Kong University of Science and Technology

3. Shagoshtasbi, Hooman. Systematic design and fabrication of a smart microfluidic single-cell electroporator.

Degree: 2013, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

► A nonlinear dynamic electro-mechanical model for a single-cell electroporation device (EP) is proposed based on micro EP experimental results. This model considers the behavior of… (more)

▼ A nonlinear dynamic electro-mechanical model for a single-cell electroporation device (EP) is proposed based on micro EP experimental results. This model considers the behavior of electromechanical components of a biological cell, the electric components of the media together with the device’s electrical components. There are four state variables for the proposed EP model including the voltage across the micro electrodes for EP, the transmembrane voltage, the average radius of pores and the cell membrane pore density. The numerical simulation of the model demonstrates good agreement with the experimental data. The predicted current responses show a much better agreement (8% error) with the micro EP experiments in comparison with the previous models. The model predicts the critical transmembrane voltage which is in good agreement with previous derived values from experiments. In addition, the proposed model can explain various aspects of structural changes of the cell membrane during different stages of electroporation, such as the Current-Voltage (I-V) characteristics of the cell membrane, the number of electropores and average pore radius. The numerical I-V characteristics of a nanoscale electropore on the cell membrane during EP illustrates the formation and expansion of the electropore during different stages of EP is analogous to the electric breakdown of the PNP junction in a semiconductor DIAC (diode for alternating current). The proposed model illustrates that the cell size is important to determine the system specifications. Based on the proposed EP model, a single-cell EP system is designed and fabricated to determine the cell size using on-chip micro Coulter counter and to apply the optimal electric field to achieve high EP efficiency and cell viability. The fabricated single-cell EP microchip is tested using HeLa cells. An EP electric field optimizer microchip is designed and fabricated to derive the optimal applied electric field for different sizes of the suspended HeLa cells. Compare to commercialized Electroporators, the fabricated device reduces the required applied voltage from several kV to several volts. The viability and permeability of HeLa cell line for the typical macro EP system are 50% and 65% respectively. For the open loop micro EP systems, the typical viable and permeabilized HeLa cells for applied electric field of 1.1 kV/cm are 76% and 75% respectively. The smart closed loop micro EP system improves the viability and permeabilization of cells to more than 90%.

Subjects/Keywords: Electroporation; Microfluidics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shagoshtasbi, H. (2013). Systematic design and fabrication of a smart microfluidic single-cell electroporator. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shagoshtasbi, Hooman. “Systematic design and fabrication of a smart microfluidic single-cell electroporator.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed September 15, 2019. https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shagoshtasbi, Hooman. “Systematic design and fabrication of a smart microfluidic single-cell electroporator.” 2013. Web. 15 Sep 2019.

Vancouver:

Shagoshtasbi H. Systematic design and fabrication of a smart microfluidic single-cell electroporator. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2019 Sep 15]. Available from: https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shagoshtasbi H. Systematic design and fabrication of a smart microfluidic single-cell electroporator. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: https://doi.org/10.14711/thesis-b1266464 ; http://repository.ust.hk/ir/bitstream/1783.1-86314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cal Poly

4. Foltz, Garrett. Algae Lysis with Pulsed Electric Fields.

Degree: MS, Electrical Engineering, 2012, Cal Poly

URL: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

► With growing interest in alternative fuels, algae lipid harvesting is seen as a possible source of biofuel. Algae species under consideration include Chlorella vulgaris,… (more)

Subjects/Keywords: Biofuel; Electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Foltz, G. (2012). Algae Lysis with Pulsed Electric Fields. (Masters Thesis). Cal Poly. Retrieved from https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

Chicago Manual of Style (16^th Edition):

Foltz, Garrett. “Algae Lysis with Pulsed Electric Fields.” 2012. Masters Thesis, Cal Poly. Accessed September 15, 2019. https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45.

MLA Handbook (7^th Edition):

Foltz, Garrett. “Algae Lysis with Pulsed Electric Fields.” 2012. Web. 15 Sep 2019.

Vancouver:

Foltz G. Algae Lysis with Pulsed Electric Fields. [Internet] [Masters thesis]. Cal Poly; 2012. [cited 2019 Sep 15]. Available from: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45.

Council of Science Editors:

Foltz G. Algae Lysis with Pulsed Electric Fields. [Masters Thesis]. Cal Poly; 2012. Available from: https://digitalcommons.calpoly.edu/theses/732 ; 10.15368/theses.2012.45

Université de Neuchâtel

5. Generelli, Silvia. Ion-selective microelectrode arrays for cell culture monitoring.

Degree: 2008, Université de Neuchâtel

URL: http://doc.rero.ch/record/10685

► The design, microfabrication and characterization of a platform comprising an array of ion-selective microelectrodes (µISE) aimed at <i>in vitro</i> cellular physiology and toxicology is described.… (more)

▼ The design, microfabrication and characterization of a platform comprising an array of ion-selective microelectrodes (µISE) aimed at <i>in vitro</i> cellular physiology and toxicology is described. This study focusses on K⁺ and Ca²⁺ monitoring in cell culture environments. A potential promising application of such a platform is based on recent findings in molecular biology, revealing connections between certain diseases, as for example some types of cancer or parkinsonism, and a malfunction in cellular ion fluxes. The silicon microfabrication of the platform allowed the realization of ion-selective microelectrode arrays comprising 16 electrodes with diameters ranging from 1.5 µm to 6 µm, and an interelectrode gap of 150 µm or 300 µm. The µISE, inspired by the classical glass capillary microelectrodes, are based on a micropipette-like channel which, in two of the three realized geometries, protrude for 5 µm from the surface and are located in a 350 µm deep cavity. In the third geometry, the micropipettes are non-protruding, completely embedded in the silicon substrate. This planar platform allowed the implementation of an array of K⁺-selective electrodes combined with planar platinum electrodes. The metallic electrodes placed near the ion-selective electrodes are designed for the application of electrical pulses for localised electroporation of cells. Preliminary tests showed the feasibility of localised electroporation, and demonstrated that the application of several electroporation pulses does not affect the functionality of K⁺ selective microelectrodes, however further investigation is necessary to optimize the electroporation protocol. The characterization of the electrodes in physiological concentration ranges was performed using electrodes of 1.5 µm diameter in contact with simple buffer calibration solutions. The observed detection limits, 10^-5 M for the K⁺ -selective electrodes and 10^-9 M for the Ca<sup²⁺ -selective electrodes, demonstrated that the µISE are adapted for the use in cell culture concentration ranges. The functional lifetime of the sensors, when conditioned in 10^-3 M KCl, or 10^-4 M CaCl₂ respectively, varied from 30 to 45 days for K⁺ -selective electrodes, but is limited to 1 to 3 days for Ca²⁺-selective electrodes. This difference in the functional lifetimes of K⁺- and Ca²⁺ -selective electrodes is due to the use of calcium-selective sensors in their non-equilibrium state. After several days of use the calcium detection limit rises to the micromolar range, which is adapted for extracellular calcium concentrations, but not for intracellular monitoring. In this case, the lifetime of the sensors is comparable to those of K⁺-selective microelectrodes. Tests performed in presence of culture medium showed a drastic diminution of the electrodes lifetime down to several days. On the other hand, a short-time contact of the sensors… Advisors/Committee Members: Nicolas-F. (Dir.).

Subjects/Keywords: electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Generelli, S. (2008). Ion-selective microelectrode arrays for cell culture monitoring. (Thesis). Université de Neuchâtel. Retrieved from http://doc.rero.ch/record/10685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Generelli, Silvia. “Ion-selective microelectrode arrays for cell culture monitoring.” 2008. Thesis, Université de Neuchâtel. Accessed September 15, 2019. http://doc.rero.ch/record/10685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Generelli, Silvia. “Ion-selective microelectrode arrays for cell culture monitoring.” 2008. Web. 15 Sep 2019.

Vancouver:

Generelli S. Ion-selective microelectrode arrays for cell culture monitoring. [Internet] [Thesis]. Université de Neuchâtel; 2008. [cited 2019 Sep 15]. Available from: http://doc.rero.ch/record/10685.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Generelli S. Ion-selective microelectrode arrays for cell culture monitoring. [Thesis]. Université de Neuchâtel; 2008. Available from: http://doc.rero.ch/record/10685

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

6. Li, Jianbo, 1980-. Modeling of electroporation mediated molecular delivery.

Degree: Mechanical and Aerospace Engineering, 2011, Rutgers University

URL: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Subjects/Keywords: Electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Jianbo, 1. (2011). Modeling of electroporation mediated molecular delivery. (Thesis). Rutgers University. Retrieved from http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Jianbo, 1980-. “Modeling of electroporation mediated molecular delivery.” 2011. Thesis, Rutgers University. Accessed September 15, 2019. http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Jianbo, 1980-. “Modeling of electroporation mediated molecular delivery.” 2011. Web. 15 Sep 2019.

Vancouver:

Li, Jianbo 1. Modeling of electroporation mediated molecular delivery. [Internet] [Thesis]. Rutgers University; 2011. [cited 2019 Sep 15]. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li, Jianbo 1. Modeling of electroporation mediated molecular delivery. [Thesis]. Rutgers University; 2011. Available from: http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000063515

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

7. Howdyshell, Marci Lynn. Micro-magnetic Structures for Biological Applications.

Degree: PhD, Physics, 2014, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

► Developments in single-molecule and single-cell experiments over the past century have provided researchers with many tools to probe the responses of cells to stresses such… (more)

Subjects/Keywords: Physics; micro-magnetic traps; electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Howdyshell, M. L. (2014). Micro-magnetic Structures for Biological Applications. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

Chicago Manual of Style (16^th Edition):

Howdyshell, Marci Lynn. “Micro-magnetic Structures for Biological Applications.” 2014. Doctoral Dissertation, The Ohio State University. Accessed September 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613.

MLA Handbook (7^th Edition):

Howdyshell, Marci Lynn. “Micro-magnetic Structures for Biological Applications.” 2014. Web. 15 Sep 2019.

Vancouver:

Howdyshell ML. Micro-magnetic Structures for Biological Applications. [Internet] [Doctoral dissertation]. The Ohio State University; 2014. [cited 2019 Sep 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613.

Council of Science Editors:

Howdyshell ML. Micro-magnetic Structures for Biological Applications. [Doctoral Dissertation]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408718613

Hong Kong University of Science and Technology

8. Yin, Guangyao. Theoretical analysis and experiments of single cell electroporation using MEMS technology.

Degree: 2010, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1097861 ; http://repository.ust.hk/ir/bitstream/1783.1-6475/1/th_redirect.html

► Electroporation is one of popular techniques to increase the permeability of cells for DNA transfer and drug delivery, in which an applied electric field induces… (more)

Subjects/Keywords: Electroporation; Microelectromechanical systems; Genetic transformation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yin, G. (2010). Theoretical analysis and experiments of single cell electroporation using MEMS technology. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1097861 ; http://repository.ust.hk/ir/bitstream/1783.1-6475/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yin, Guangyao. “Theoretical analysis and experiments of single cell electroporation using MEMS technology.” 2010. Thesis, Hong Kong University of Science and Technology. Accessed September 15, 2019. https://doi.org/10.14711/thesis-b1097861 ; http://repository.ust.hk/ir/bitstream/1783.1-6475/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yin, Guangyao. “Theoretical analysis and experiments of single cell electroporation using MEMS technology.” 2010. Web. 15 Sep 2019.

Vancouver:

Yin G. Theoretical analysis and experiments of single cell electroporation using MEMS technology. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2010. [cited 2019 Sep 15]. Available from: https://doi.org/10.14711/thesis-b1097861 ; http://repository.ust.hk/ir/bitstream/1783.1-6475/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yin G. Theoretical analysis and experiments of single cell electroporation using MEMS technology. [Thesis]. Hong Kong University of Science and Technology; 2010. Available from: https://doi.org/10.14711/thesis-b1097861 ; http://repository.ust.hk/ir/bitstream/1783.1-6475/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Nova

9. Fernandes, Catarina Sofia Rodrigues. Linking the embryonic clock with temporal collinearity of Hox gene activation.

Degree: 2010, Universidade Nova

URL: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

►

Dissertação apresentada para a obtenção do Grau de Mestre em Genética Molecular e Biomedicina, pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Somitogenesis… (more)

Subjects/Keywords: Hairy1; Brachyury; Electroporation; Molecular clock

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernandes, C. S. R. (2010). Linking the embryonic clock with temporal collinearity of Hox gene activation. (Thesis). Universidade Nova. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernandes, Catarina Sofia Rodrigues. “Linking the embryonic clock with temporal collinearity of Hox gene activation.” 2010. Thesis, Universidade Nova. Accessed September 15, 2019. http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernandes, Catarina Sofia Rodrigues. “Linking the embryonic clock with temporal collinearity of Hox gene activation.” 2010. Web. 15 Sep 2019.

Vancouver:

Fernandes CSR. Linking the embryonic clock with temporal collinearity of Hox gene activation. [Internet] [Thesis]. Universidade Nova; 2010. [cited 2019 Sep 15]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernandes CSR. Linking the embryonic clock with temporal collinearity of Hox gene activation. [Thesis]. Universidade Nova; 2010. Available from: http://www.rcaap.pt/detail.jsp?id=oai:run.unl.pt:10362/5806

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

10. Sano, Michael B. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.

Degree: PhD, Biomedical Engineering, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/77122

► The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare… (more)

▼ The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare mammalian cells dispersed within heterogeneous populations. Relevant examples of these target cells are stem cells within a differentiated population, circulating tumor cells (CTCs) in the blood stream, and tumor initiating cells (TICs) in a population of benign cancer cells. The ability to isolate any of these rare cells types with high efficiency will directly lead to advances in tissue engineering, cancer detection, and individualized medicine. This work lead directly to the development of a new cell manipulation technique, termed contactless dielectrophoresis (cDEP). In this technique, cells are isolated from direct contact with metal electrodes by employing fluid electrode channels filled with a highly conductive media. Thin insulating barriers, approximately 20 μm, serve to isolate the fluid electrode channels from the low conductivity sample buffer. The insulating barriers in a fluid-electrical system create a number of unique and interesting challenges from an electrical engineering standpoint. Primarily, they block the flow of DC currents and create a non-constant frequency response which can confound experimental results attempting to characterize the electrical characteristics of cells. Due to these, and other, considerations, the use of high-voltage high-frequency signals are necessary to successfully manipulate cells. The effect of these high frequency fields on cells are studied and applied to microfluidic and tissue level applications. In later chapters, theoretical and experimental results show how high frequency and pulsed electric fields can ablate cells and tissue. Understanding the parameters necessary to electroporate cells aids in the development of cDEP devices where this phenomenon is undesirable and gives insight towards the development of new cancer ablation therapies where targeted cell death is sought after. This work shows that there exists a finite frequency spectrum over which cDEP devices can operate in which cells are minimally affected by the induced electric fields. Finally, lessons learned in the course of the development of cDEP were adapted and applied towards cancer ablation therapies where electroporation are favorable. It was found that bursts of high frequency pulsed electric fields can successfully kill cells and ablate tissue volumes through a process termed High Frequency Irreversible Electroporation (H-FIRE). This technique is advantageous as these waveforms mitigate or eliminate muscle contractions associated with traditional IRE technologies. Similarly, the use of fluid electrodes in cDEP inspired the use of an organs vascular system as the conductive pathway to deliver pulses. This novel approach allows for the ablation of large volumes of tissue without the use of puncturing electrodes. These techniques are currently undergoing evaluation in tissue engineering applications and pre-clinical… Advisors/Committee Members: Davalos, Rafael V. (committeechair), Lu, Chang-Tien (committee member), Robertson, John L. (committee member), Rossmeisl, John H. Jr. (committee member), Brown, Gary S. (committee member), Soker, Shay (committee member).

Subjects/Keywords: Irreversible Electroporation; Contactless Dielectrophoresis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sano, M. B. (2012). Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77122

Chicago Manual of Style (16^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Doctoral Dissertation, Virginia Tech. Accessed September 15, 2019. http://hdl.handle.net/10919/77122.

MLA Handbook (7^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Web. 15 Sep 2019.

Vancouver:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Internet] [Doctoral dissertation]. Virginia Tech; 2012. [cited 2019 Sep 15]. Available from: http://hdl.handle.net/10919/77122.

Council of Science Editors:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Doctoral Dissertation]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/77122

University of Waterloo

11. Movahed, Saeid. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/7149

► In this thesis, underlying concepts of transport phenomena through generated nanopores on a cell membrane during electroporation were studied. A comprehensive literature review was performed… (more)

▼ In this thesis, underlying concepts of transport phenomena through generated nanopores on a cell membrane during electroporation were studied. A comprehensive literature review was performed to find the pros and cons of the previous works and consequently extensive studies were accomplished to explain shortcomings of the former studies on this topic. The membrane permeabilization of the single cell located in the microchannel was studied, and the effects of microchannel’s wall and electrode size were investigated on cell electroporation. It was studied how the electrical (e.g., strength of the electric pulse) and geometrical parameters (e.g., microchannel height and electrode size) affect size, location, and number of created hydrophilic pores on the cell membrane. Because of a transmembrane potential, the electrokinetic effects have decisive influence on the transport process through the created nanopores. A comprehensive study was performed to explain the electrokinetic transport through the nanochannels. Effects of surface electric charge and radius of the nanochannel on the electric potential, liquid flow, and ionic transport were investigated. Unlike microchannels, the electric potential field, ionic concentration field, and velocity field are strongly size-dependent in the nanochannels. They are also affected by the surface electric charge of the nanochannel. More counter ions than co-ions are transported through the nanochannel. The ionic concentration enrichment at the entrance and the exit of the nanochannel is completely evident from the simulation results. The study also shows that the fluid velocity in the nanochannel is higher when the surface electric charge is stronger, or the radius of the nanochannel is larger. The obtained model of the electrokinetic effects in the nanochannels was utilized to examine the ionic mass transfer and the fluid flow through the generated hydrophilic nanopores of the cell membrane during electroporation. The results showed how the electric potential, velocity field, and ionic concentration vary with the size and angular position of the generated nanopores of the cell membrane. It was also shown that, in the presence of the electric pulse, the electrokinetic effects (the electroosmosis and the electrophoresis) had significant influences on the ionic mass transfer through the nanopores, while the effect of diffusion on the ionic mass flux was negligible in comparison with the electrokinetics. Increasing the radius of the nanopores intensified the effect of convection (electroosmosis) in comparison with the electrophoresis on the ionic flux. Furthermore, the electrokinetic motion of the nanoparticle through the nanochannel was investigated to mimic inserting the nanoscale biological samples, such as QDots and DNAs, through the created nanopores on the cell membrane. It was proved that, because of the large applied electric field over the nanochannel, the impact of the Brownian force was negligible in comparison with the electrophoretic and the hydrodynamic forces. It…

Subjects/Keywords: electroporation; electrokinetics; nanopore; nanochannel

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Movahed, S. (2012). Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Movahed, Saeid. “Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.” 2012. Thesis, University of Waterloo. Accessed September 15, 2019. http://hdl.handle.net/10012/7149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Movahed, Saeid. “Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation.” 2012. Web. 15 Sep 2019.

Vancouver:

Movahed S. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2019 Sep 15]. Available from: http://hdl.handle.net/10012/7149.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Movahed S. Electrokinetic Transport Process in Nanopores Generated on Cell Membrane during Electroporation. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/7149

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

12. Demiryurek, Yasir, 1985-. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.

Degree: MS, Mechanical and Aerospace Engineering, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

► Electroporation-mediated molecular delivery is of interest for many drug-delivery and gene-therapy applications. Recent studies have shown that a two-pulse protocol consisting of a short-duration high-voltage… (more)

▼ Electroporation-mediated molecular delivery is of interest for many drug-delivery and gene-therapy applications. Recent studies have shown that a two-pulse protocol consisting of a short-duration high-voltage first pulse followed by a longer, low-voltage second pulse can increase the efficiency of molecular delivery and preserve more cells alive with suitably chosen pulsing parameters. In this work we investigate the effects of the first and second pulses’ field strength and the inter-pulse delay time on the delivery of two different-sized Fluorescein-Dextran conjugates (10 kDa and 70 kDa). A series of two-pulse electroporation experiments were performed on 3T3 mouse fibroblast cells, with an alternating-current first pulse to permeabilize the cell, followed by a direct-current second pulse to electrophoretically deliver the target molecule into the cell. Our results showed that the delivery amounts of Fluorescein-Dextran varies strongly with the first pulse's field strength and target molecule size. By varying the delay times between two pulses, it is shown that the delivered intracellular concentration of Fluorescein-Dextran decreased linearly with the logarithm of delay time. The data also indicate that membrane resealing after electropermeabilization occurs very rapidly, but that a non-negligible fraction of the pores can be reopened by the second pulse for times on the order of 100 s. The role of the second pulses is seen to be more than just electrophoresis, with a minimum threshold field strength required to reopen nano-sized pores or defects remaining from the first pulse. These results suggest that membrane electroporation, sealing, and reporation is a complex process that has both short-term and long-term components, which may in part explain the wide variation in membrane-resealing times reported in the literature. Advisors/Committee Members: Shan, Jerry (chair), Lin, Hao (internal member), Zahn, Jeffrey (outside member), Shreiber, David (outside member).

Subjects/Keywords: Electroporation; Fluorescein; Gene therapy

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Demiryurek, Yasir, 1. (2014). Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

Chicago Manual of Style (16^th Edition):

Demiryurek, Yasir, 1985-. “Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.” 2014. Masters Thesis, Rutgers University. Accessed September 15, 2019. https://rucore.libraries.rutgers.edu/rutgers-lib/45233/.

MLA Handbook (7^th Edition):

Demiryurek, Yasir, 1985-. “Transport and resealing dynamics of two pulse electroporation mediated molecular delivery.” 2014. Web. 15 Sep 2019.

Vancouver:

Demiryurek, Yasir 1. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2019 Sep 15]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/.

Council of Science Editors:

Demiryurek, Yasir 1. Transport and resealing dynamics of two pulse electroporation mediated molecular delivery. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45233/

The Ohio State University

13. Fei, Zhengzheng. Membrane Sandwich Electroporation for In Vitro Gene Delivery.

Degree: PhD, Chemical Engineering, 2009, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1251384862

► Gene therapy is the delivery of therapeutic genes into cells and tissues with the aim of treating and curing a disease. As an enhanced understanding… (more)

▼ Gene therapy is the delivery of therapeutic genes into cells and tissues with the aim of treating and curing a disease. As an enhanced understanding of the roles of genes in health and disease, gene therapy is showing promise against various diseases such as cancer, diabetes, Parkinson's disease, and several inherited physiological defects. Viral transduction is very efficient, but safety issues, such as immune and inflammatory responses, have hampered their clinical uses in humans. Non-viral methods, including either chemical transfection with cationic lipids/polymers or physical transfection using electroporation/microinjection, are becoming attractive approaches. Electroporation is one of the most popular non-viral gene transfer methods for in vitro cell transfection. Initial studies with electroporation experienced very low transfection efficiencies and cell viability, severely limiting the development of this technology. The emergence of nucleofection (a modified electroporation technology) provided an efficient means for transfecting cells in vitro. However, nucleofection still encounters many limitations such as the large number of cells required and high cost involved. Moreover, cell viability is still an issue due to the high electric voltage used and the non-uniform electric field strength distribution generated during the process. To address these problems, we propose to develop an electroporation system based on an innovative micro-/nanoengineering technology for in vitro gene delivery. In our approach, electroporation is carried out in a mild and uniform electric field, with potential for a wider process window that can be generated to cover a wide range of cell lines and even primary cells. A new membrane sandwich electroporation (MSE) approach was demonstrated using plasmids GFP and SEAP as model materials. NIH 3T3 fibroblasts were tested and a significant improvement in transgene expression was observed compared to current electroporation techniques. In the MSE method, the focused electric field enhances cell permeabilization at a low electric voltage, leading to high cell viability; more important, the sandwich membrane configuration is able to provide better gene confinement near the cell surface, facilitating gene delivery into the cells. Next, we demonstrated the use of femtosecond laser fabricated micro-nozzle arrays on a gelatin-coated PET membrane for MSE. Using micro-nozzle array enhanced MSE, we observed high and uniform gene transfection, and good cell viability of mouse embryonic stem (ES) cells compared to the bulk electroporation. Since typically cells or tissues from the patients are very limited and therapeutic materials such as plasmids and oligonucleotides are very expensive, the ability to treat a small number of cells (i.e. a hundred) offers great potential to work with hard-to-harvest patient cells for patient-specific ex vivo gene therapy and in vitro pharmaceutical kinetic studies. Numerical calculation of transmembrane potential qualitatively explains the observed differences… Advisors/Committee Members: Lee, Ly James (Advisor).

Subjects/Keywords: electroporation; gene delivery; cell culture; stem cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fei, Z. (2009). Membrane Sandwich Electroporation for In Vitro Gene Delivery. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1251384862

Chicago Manual of Style (16^th Edition):

Fei, Zhengzheng. “Membrane Sandwich Electroporation for In Vitro Gene Delivery.” 2009. Doctoral Dissertation, The Ohio State University. Accessed September 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1251384862.

MLA Handbook (7^th Edition):

Fei, Zhengzheng. “Membrane Sandwich Electroporation for In Vitro Gene Delivery.” 2009. Web. 15 Sep 2019.

Vancouver:

Fei Z. Membrane Sandwich Electroporation for In Vitro Gene Delivery. [Internet] [Doctoral dissertation]. The Ohio State University; 2009. [cited 2019 Sep 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1251384862.

Council of Science Editors:

Fei Z. Membrane Sandwich Electroporation for In Vitro Gene Delivery. [Doctoral Dissertation]. The Ohio State University; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1251384862

Hong Kong University of Science and Technology

14. Gao, Wenhui. Study of DNA-uptake mechanisms in electroporation.

Degree: 1998, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

► Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by… (more)

▼ Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by cells, however, have not yet been clearly understood. In this thesis, we have summarized a series of experiment designed to examine the DAN- uptake mechanisms. In addition, cell treatments that can enhance the efficiency of electroporation are also discussed. The results show that after the application of electric field the additional treatment by using a recovery medium containing proper amounts of divalent ions can significantly increase the transfection efficiency in 3T6, Pt K2 and SK-N-SH cells. Several factors, including the strength of electric field, recovery time and concentrations of Ca++ or Mg++ were examined. The reason for increasing the transfection efficiency is believed to be mainly caused by promoting the entry of DNA through the cell membrane, rather than by enhancing the expression of the transferred gene. It has been suggested that DNA-uptake is a multiple- step process. (1) DNA may be driven into the cell by an electrophoretic force when the membrane pores were opened during the application of the electric field. (2) After the electric field was turned off, DNA may continue to diffuse into the cell before the membrane pores were closed. (3) Finally, DNA may be carried in the cell by endocytosis after the membrane pores were resealed. In this research, we have designed specific experiments to test each of these steps. Our results support steps (1) and (3), but not step (2). We found that the kinetics of membrane pore resealing depends strongly on the size of the transport molecules. Thus, uptake of large molecules such as FITC labeled dextran (70 kD) or DNA stopped almost within seconds after the electric field was turned off. In summary, we propose that the DNA-uptake mechanisms in electroporation involve a fast step of DNA entrance driven by the electric field, and a relatively slower process of DNA-uptake by endocytosis following the turn-off of the electric pulses. The experimental evidence will be discussed in detail in this thesis.

Subjects/Keywords: Electroporation; DNA; Transfection

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gao, W. (1998). Study of DNA-uptake mechanisms in electroporation. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Thesis, Hong Kong University of Science and Technology. Accessed September 15, 2019. https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Web. 15 Sep 2019.

Vancouver:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Internet] [Thesis]. Hong Kong University of Science and Technology; 1998. [cited 2019 Sep 15]. Available from: https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Thesis]. Hong Kong University of Science and Technology; 1998. Available from: https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

15. Yang, Fan. Fabrication and characterization of a micro electroporation cell chip.

Degree: 2004, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

► Electroporation is one of the most important gene transfer methods in biotechnology, which employs electrical pulse applied across a cell for cell membrane permeabilization. Compared… (more)

Subjects/Keywords: Electroporation; Cytology – Technique

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, F. (2004). Fabrication and characterization of a micro electroporation cell chip. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Fan. “Fabrication and characterization of a micro electroporation cell chip.” 2004. Thesis, Hong Kong University of Science and Technology. Accessed September 15, 2019. https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Fan. “Fabrication and characterization of a micro electroporation cell chip.” 2004. Web. 15 Sep 2019.

Vancouver:

Yang F. Fabrication and characterization of a micro electroporation cell chip. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2004. [cited 2019 Sep 15]. Available from: https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang F. Fabrication and characterization of a micro electroporation cell chip. [Thesis]. Hong Kong University of Science and Technology; 2004. Available from: https://doi.org/10.14711/thesis-b818953 ; http://repository.ust.hk/ir/bitstream/1783.1-5264/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

16. Yuan, Hanwen. Fabrication and characterization of a mcro/nanofluidic platform for electroporation.

Degree: PhD, 2017, University of Louisville

URL: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

► For traditional electroporation devices, there are a number of problems associated with these devices such as insufficient understanding of its theoretical mechanism, low cell… (more)

▼ For traditional electroporation devices, there are a number of problems associated with these devices such as insufficient understanding of its theoretical mechanism, low cell viability, inadequate electroporation efficiency, excess voltage applied to generate required electric field due to the large size of these devices and sample contamination. Although newly developed microfluidic electroporation devices have solved most of the above existing problems in traditional bulk electroporation devices, they appear to lack the ability to control the precise dose of biomolecules or genes transfecting into cells and, from a manufacturing perspective, the fabrication methods do not enable repeatable production of such devices on the large scale. Here, we introduce a new, repeatable method for fabricating 3-D Micro/Nanofluidic electroporation platforms and characterize these platforms to demonstrate their ability to electroporate live cells. Some of the new methods developed in this work include a direct-write fiber technique via three-axis robotic dispensing system, dry film resist photolithography, film-to-film bonding and replica molding to create the desired electroporation platform. A robotic dispensing system was utilized to control the fiber diameter, which was determined vii by the: 1) prescribed dispense time; 2) pressure of the dispensing system valve; 3) rate at which the stage traversed; 4) diameter of the dispensing tip; 5) polymer solution viscosity and surface tension; and, 6) programmed drawing length. Thin dry film photoresist was utilized to replace liquid photoresist in order to achieve high-quality film-to-film bonding after drawing nanofibers onto one substrate containing the thin-film structure. Polydimethylsiloxane (PDMS) was employed as the bulk material to fabricate the target micro/nano electroporation substrate using replica molding and micro/nanofibers etching. Characterization of the direct-write fiber technique via robotic dispensing system to acquire suspended and complex fibers of the required dimension repeatedly under prescribed conditions were completed. Combining this fiber direct-write method and traditional clean room techniques, a total of 18 micro- to nano-scale electroporation devices (6 for each group of 1 ìm, 500 nm, and 300 nm diameter) were successfully developed and mass produced in two weeks with relatively high repeatability (within 20% of the design). Finally, metrology and characterization studies were performed on the electroporation platforms to validate the micro/nanochannel’s existence and its connectivity to two micro-chambers. Furthermore, biomolecules and other fluorescent particles were successfully transported through the micro/nanochannel and transferred (via electroporation) into the cells. Preliminary results of electroporation experiment performed on this micro/nano-electroporation platform illustrated that the duration of the entire electroporation process was significantly shorter than times reported previously by other investigator’s nano-electroporation… Advisors/Committee Members: Keynton, Robert, Williams, Stuart, Brehob, Ellen, Cohn, Robert, Sumanasekera, Gamini, El-Baz, Ayman.

Subjects/Keywords: electroporation; nanochannel; microfluidic; cell; Nanoscience and Nanotechnology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yuan, H. (2017). Fabrication and characterization of a mcro/nanofluidic platform for electroporation. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

Chicago Manual of Style (16^th Edition):

Yuan, Hanwen. “Fabrication and characterization of a mcro/nanofluidic platform for electroporation.” 2017. Doctoral Dissertation, University of Louisville. Accessed September 15, 2019. 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757.

MLA Handbook (7^th Edition):

Yuan, Hanwen. “Fabrication and characterization of a mcro/nanofluidic platform for electroporation.” 2017. Web. 15 Sep 2019.

Vancouver:

Yuan H. Fabrication and characterization of a mcro/nanofluidic platform for electroporation. [Internet] [Doctoral dissertation]. University of Louisville; 2017. [cited 2019 Sep 15]. Available from: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757.

Council of Science Editors:

Yuan H. Fabrication and characterization of a mcro/nanofluidic platform for electroporation. [Doctoral Dissertation]. University of Louisville; 2017. Available from: 10.18297/etd/2757 ; https://ir.library.louisville.edu/etd/2757

Case Western Reserve University

17. Kim, Seok Chan. ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS.

Degree: MSs (Engineering), Biomedical Engineering, 2008, Case Western Reserve University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1196122659

► A strong internal defibrillation shock may damage the heart via disruption of cell membranes (electroporation). The spatial distribution and extent of electroporation were characterized in… (more)

Subjects/Keywords: Engineering, Biomedical; Electroporation; Defibrillation; Myocardial Infarction

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, S. C. (2008). ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS. (Masters Thesis). Case Western Reserve University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1196122659

Chicago Manual of Style (16^th Edition):

Kim, Seok Chan. “ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS.” 2008. Masters Thesis, Case Western Reserve University. Accessed September 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1196122659.

MLA Handbook (7^th Edition):

Kim, Seok Chan. “ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS.” 2008. Web. 15 Sep 2019.

Vancouver:

Kim SC. ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS. [Internet] [Masters thesis]. Case Western Reserve University; 2008. [cited 2019 Sep 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1196122659.

Council of Science Editors:

Kim SC. ELECTROPORATION BY STRONG INTERNAL DEFIBRILLATION SHOCK IN INTACT STRUCTURALLY NORMAL AND CHRONICALLY INFARCTED RABBIT HEARTS. [Masters Thesis]. Case Western Reserve University; 2008. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1196122659

Penn State University

18. Bae, Chilman. ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE.

Degree: PhD, Bioengineering, 2009, Penn State University

URL: https://etda.libraries.psu.edu/catalog/9903

► An integrated and automated micropipette electrode (ME)-based single-cell electroporation (SCE) system was developed for rapid, efficient, cell selective and non-invasive delivery of fluorescent compounds into… (more)

▼ An integrated and automated micropipette electrode (ME)-based single-cell electroporation (SCE) system was developed for rapid, efficient, cell selective and non-invasive delivery of fluorescent compounds into adherent endothelial cells. Key features include software-controlled pulse waveform generation, continuous monitoring of electroporation events, feedback-controlled motorized micromanipulation, image capture, and data acquisition. The main innovations of automation are image-based cell selection, 40nm-precision feedback control of ME approach, and a method to prescribe the applied membrane potential. Comparisons of experiment measurements and finite element analysis of cleft resistance enabled identification of three distinct phases of pipette positioning: positioning phase, approach phase, and indenting phase. Computational simulations were also performed to determine the quantitative relationships of cleft size and resistance, transmembrane potential (TP) and cleft size, and TP and electrode axial distance. The simulation also shows that pores are focused on the membrane area corresponding to the inner pipette lumen. In addition, we quantitatively determined membrane tension, stress, and strain distributions in the vicinity of a nanoelectrode using finite element analysis of a multiscale electro-mechanical model which consisted of pipette, media, membrane, actin cortex, and cytoplasm. Results suggest that nanopipette electrodes (nE) provide a new non-contact method to deliver physiological stresses directly to membranes in a focused and controlled manner, thus providing the quantitative foundation for micreoelectrotension. Finally, we developed a new simple method for dynamic deformation spectroscopy with a functionalized nE. The electrical and mechanical characteristics of the system and the system resolution were tested. The results suggest that the adhesion of a nanopipette electrode tip onto the cell surface is dependent on the nanopipette electrode tip size, the fibronectin concentration for coating the nanopipette electrode tip, and the reaction time. This system has high versatility because of its simplicity, easy of interpretation, and non-contact analysis of surface properties.

Subjects/Keywords: electroporation; mechanotransduction; micropipette; Cell membrane; endothelial cell

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bae, C. (2009). ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE. (Doctoral Dissertation). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/9903

Chicago Manual of Style (16^th Edition):

Bae, Chilman. “ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE.” 2009. Doctoral Dissertation, Penn State University. Accessed September 15, 2019. https://etda.libraries.psu.edu/catalog/9903.

MLA Handbook (7^th Edition):

Bae, Chilman. “ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE.” 2009. Web. 15 Sep 2019.

Vancouver:

Bae C. ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE. [Internet] [Doctoral dissertation]. Penn State University; 2009. [cited 2019 Sep 15]. Available from: https://etda.libraries.psu.edu/catalog/9903.

Council of Science Editors:

Bae C. ELECTRICAL, CHEMICAL, AND MECHANICAL MANIPULATION OF THE ENDOTHELIAL CELL SURFACE WITH A MULTIFUNCTIONAL NANOPIPETTE. [Doctoral Dissertation]. Penn State University; 2009. Available from: https://etda.libraries.psu.edu/catalog/9903

The Ohio State University

19. Shi, Junfeng, Leng. Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering.

Degree: PhD, Mechanical Engineering, 2017, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1503059915552435

► Delivery of exogenous materials plays a key role a diversity of biomedical and pharmaceutical applications. Precise, rapid and benign introduction of biomolecules into a large… (more)

▼ Delivery of exogenous materials plays a key role a diversity of biomedical and pharmaceutical applications. Precise, rapid and benign introduction of biomolecules into a large population of cells has thus long fascinates the scientific community. To circumvent the safety concern raised by viral vectors, a variety of non-viral delivery approaches have been developed, including chemical carrier-mediated methods and physical membrane-penetrating methods. Although novel synthetic lipoplex and polyplex nanocarriers provide opportunities for targeted delivery both in vivo and in vitro, the delivery process is slow and inefficient – only a small fraction of encapsulated materials is eventually delivered to cell interior after endocytosis followed by endosomal escape. Electroporation has been the leading physical delivery method since its invention about four decades ago. Bulk electroporation (BEP) is the commercially available electroporation system. While BEP offers the advantage of simplicity to use without any package of delivery materials, it suffers from low cell viability and significant cell-to-cell variation owing to the non-uniform electric field imposed on the cells randomly suspended in the bulk solution.A rapid growth of microfluidics has been witnessed since 2000, as microfabrication techniques get mature. The spatial confinement offers an opportunity to accurately control over fluid transport as well as the electric field distribution.In this work, a miniaturized version of electroporation – nano-electroporation (NEP) was integrated in three biochip platforms, which could revitalize this classic physical delivery method. Large-scale single-clone-resolution living cell interrogation, including single- cell motility, drug resistance, and underlying molecular mechanisms, based on a 3D NEP- cell migration platform was demonstrated using a clinically relevant study of patient-derive glioma stem cells (GSCs) in the highly lethal brain tumor – glioblastoma. This platform was utilized to follow the dynamics of phenotypic shift of cancer stem cells and thus has potential to shed a new perspective for current solid tumor research and help develop novel therapeutic strategy specifically targeting a subpopulation with high plasticity within cancer stem cells.To enhance the transfection efficiency and the versatility of transfected cell types (i.e., both adherent and suspension cells) in the 3D NEP platform, a scalable microfluidic device enabling rapid, massive and accurate hydrodynamic-based cell trapping critical for large-scale 3D NEP cell transfection was introduced. Various cargoes from large plasmids to small oligos have been shown to be uniformly NEP-delivered into the trapped cell array in a controllable and uniform manner. Such innovative micro-/nano-technology-enabled cell transfection platform could be valuable for cell reprogramming and ex vivo cancer adoptive immunotherapies.For the first time, we reported that NEP can not only serve as a unique gene delivery tool, capable of deterministic and benign… Advisors/Committee Members: Lee, James (Advisor).

Subjects/Keywords: Mechanical Engineering; Nano-electroporation, Microfluidics, Microfabrication

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shi, Junfeng, L. (2017). Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1503059915552435

Chicago Manual of Style (16^th Edition):

Shi, Junfeng, Leng. “Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering.” 2017. Doctoral Dissertation, The Ohio State University. Accessed September 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503059915552435.

MLA Handbook (7^th Edition):

Shi, Junfeng, Leng. “Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering.” 2017. Web. 15 Sep 2019.

Vancouver:

Shi, Junfeng L. Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering. [Internet] [Doctoral dissertation]. The Ohio State University; 2017. [cited 2019 Sep 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1503059915552435.

Council of Science Editors:

Shi, Junfeng L. Development of Nanoelectroporation-based Biochips for Living Cell Interrogation and Extracellular Vesicle Engineering. [Doctoral Dissertation]. The Ohio State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1503059915552435

Virginia Tech

20. Hassanisaber, Hamid. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.

Degree: MS, Chemical Engineering, 2014, Virginia Tech

URL: http://hdl.handle.net.ezproxy.lib.vt.edu/10919/25112

► Electroporation is an efficient, low-toxic physical method which is used to deliver impermeant macromolecules such as genes and drugs into cells. Genetic modification of the… (more)

▼ Electroporation is an efficient, low-toxic physical method which is used to deliver impermeant macromolecules such as genes and drugs into cells. Genetic modification of the cell is critical for many cell and gene therapy techniques. Common electroporation protocols can only handle small volumes of cell samples. Also, most of the conventional electroporation methods require expensive and sophisticated electro-pulsation equipment. In our lab, we have developed new electroporation methods conducted in microfluidic devices. In microfluidic-base electroporation, exogenous macromolecules can be delivered into cells continuously. Flow-through electroporation systems can overcome the issue of low sample volume limitation. In addition, in our method, electro-pulsation can be done by using a simple dc power supply, without the need for any extra equipment. Furthermore, our microfluidic chips are completely disposable and cheap to produce. We show that electroporation and electroporation-based gene delivery can be conducted employing tapered asymmetric curving channels. The size variation in the channel's cross-sectional area makes it possible to produce electric pulses of various parameters by using a dc power supply. We successfully delivered Enhanced Green Fluorescent Protein, EGFP, plasmid DNA into Chinese Hamster Ovary, CHO-K1, cells in our microfluidic chips. We show that the particles/cells undergo Dean flow in our asymmetric curving channels. We demonstrate that there are three main regimes for particle motion in our channels. At low flow rates (from 0 to ~75μl/min) cells do not focus and they randomly follow stream lines. However, as flow rate increases (~75 to 500μl/min), cells begin to focus into one line and they follow a single path throughout the micro-channel. When flow rate exceeds ~500μl/min, cells do not follow a single line and demonstrate more complex pattern. We show that the electric parameters affect the transfection efficiency and cell viability. Higher electric field intensity results in higher transfection efficiency. This is also true in the cases with longer electroporation duration time. In our experimental work, we executed flow-through electroporation for various duration times (t = 2 ms, 5 ms, and 7 ms), and at various electric field intensities (from 300 to 2200 V/cm) while we utilized different flow rates as well, i. e. 150 μl/min (focused flow) and 600 μl/min (complex flow). To explore the impact of individual electric pulse length and electric pulse number on electroporation results, we designed control channels with straight narrow sections. Cells experience different hydrodynamic forces in straight channels compared to curving channels. Flow pattern and cell focusing were also studied in control channels as well. Also, electroporation on CHO-K1 cells was successfully conducted in control channels. The hydrodynamic forces under the conditions we used do not appear to show substantial impact on transfection efficiency. Advisors/Committee Members: Lu, Chang (committeechair), Ducker, William A (committee member), Baird, Donald G (committee member).

Subjects/Keywords: Microfluidics; Electroporation; Particle focusing; CHO-K1 cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hassanisaber, H. (2014). Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net.ezproxy.lib.vt.edu/10919/25112

Chicago Manual of Style (16^th Edition):

Hassanisaber, Hamid. “Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.” 2014. Masters Thesis, Virginia Tech. Accessed September 15, 2019. http://hdl.handle.net.ezproxy.lib.vt.edu/10919/25112.

MLA Handbook (7^th Edition):

Hassanisaber, Hamid. “Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.” 2014. Web. 15 Sep 2019.

Vancouver:

Hassanisaber H. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. [Internet] [Masters thesis]. Virginia Tech; 2014. [cited 2019 Sep 15]. Available from: http://hdl.handle.net.ezproxy.lib.vt.edu/10919/25112.

Council of Science Editors:

Hassanisaber H. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. [Masters Thesis]. Virginia Tech; 2014. Available from: http://hdl.handle.net.ezproxy.lib.vt.edu/10919/25112

21. Frazier, Hilaree Noele. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.

Degree: MS, Biological Sciences, 2016, Encompass Digital Archive, Eastern Kentucky University

URL: https://encompass.eku.edu/etd/365

► The process of using electroporation to introduce plasmid DNA into host cells is a valuable molecular technique that is increasingly employed in labs worldwide.… (more)

Subjects/Keywords: bacteria; electroporation; listeria; plasmid; Microbiology; Molecular Biology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frazier, H. N. (2016). Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. (Masters Thesis). Encompass Digital Archive, Eastern Kentucky University. Retrieved from https://encompass.eku.edu/etd/365

Chicago Manual of Style (16^th Edition):

Frazier, Hilaree Noele. “Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.” 2016. Masters Thesis, Encompass Digital Archive, Eastern Kentucky University. Accessed September 15, 2019. https://encompass.eku.edu/etd/365.

MLA Handbook (7^th Edition):

Frazier, Hilaree Noele. “Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes.” 2016. Web. 15 Sep 2019.

Vancouver:

Frazier HN. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. [Internet] [Masters thesis]. Encompass Digital Archive, Eastern Kentucky University; 2016. [cited 2019 Sep 15]. Available from: https://encompass.eku.edu/etd/365.

Council of Science Editors:

Frazier HN. Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes. [Masters Thesis]. Encompass Digital Archive, Eastern Kentucky University; 2016. Available from: https://encompass.eku.edu/etd/365

Virginia Tech

22. Dunlap-Brown, Marya. The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes.

Degree: MS, Dairy Science, 2010, Virginia Tech

URL: http://hdl.handle.net/10919/33743

► Pronuclear stage murine embryos received electrical stimulation in 5, 10, or 20 Âµs pulse lengths, and 0, 100, 200, 250, 300 or 400 voltages.… (more)

Subjects/Keywords: Transgenic; Electroporation; Embryo; EGFP; Mice; Microinjection

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dunlap-Brown, M. (2010). The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/33743

Chicago Manual of Style (16^th Edition):

Dunlap-Brown, Marya. “The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes.” 2010. Masters Thesis, Virginia Tech. Accessed September 15, 2019. http://hdl.handle.net/10919/33743.

MLA Handbook (7^th Edition):

Dunlap-Brown, Marya. “The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes.” 2010. Web. 15 Sep 2019.

Vancouver:

Dunlap-Brown M. The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes. [Internet] [Masters thesis]. Virginia Tech; 2010. [cited 2019 Sep 15]. Available from: http://hdl.handle.net/10919/33743.

Council of Science Editors:

Dunlap-Brown M. The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes. [Masters Thesis]. Virginia Tech; 2010. Available from: http://hdl.handle.net/10919/33743

Virginia Tech

23. Hassanisaber, Hamid. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.

Degree: MS, Chemical Engineering, 2014, Virginia Tech

URL: http://hdl.handle.net/10919/25112

► Electroporation is an efficient, low-toxic physical method which is used to deliver impermeant macromolecules such as genes and drugs into cells. Genetic modification of the… (more)

▼ Electroporation is an efficient, low-toxic physical method which is used to deliver impermeant macromolecules such as genes and drugs into cells. Genetic modification of the cell is critical for many cell and gene therapy techniques. Common electroporation protocols can only handle small volumes of cell samples. Also, most of the conventional electroporation methods require expensive and sophisticated electro-pulsation equipment. In our lab, we have developed new electroporation methods conducted in microfluidic devices. In microfluidic-base electroporation, exogenous macromolecules can be delivered into cells continuously. Flow-through electroporation systems can overcome the issue of low sample volume limitation. In addition, in our method, electro-pulsation can be done by using a simple dc power supply, without the need for any extra equipment. Furthermore, our microfluidic chips are completely disposable and cheap to produce. We show that electroporation and electroporation-based gene delivery can be conducted employing tapered asymmetric curving channels. The size variation in the channel's cross-sectional area makes it possible to produce electric pulses of various parameters by using a dc power supply. We successfully delivered Enhanced Green Fluorescent Protein, EGFP, plasmid DNA into Chinese Hamster Ovary, CHO-K1, cells in our microfluidic chips. We show that the particles/cells undergo Dean flow in our asymmetric curving channels. We demonstrate that there are three main regimes for particle motion in our channels. At low flow rates (from 0 to ~75μl/min) cells do not focus and they randomly follow stream lines. However, as flow rate increases (~75 to 500μl/min), cells begin to focus into one line and they follow a single path throughout the micro-channel. When flow rate exceeds ~500μl/min, cells do not follow a single line and demonstrate more complex pattern. We show that the electric parameters affect the transfection efficiency and cell viability. Higher electric field intensity results in higher transfection efficiency. This is also true in the cases with longer electroporation duration time. In our experimental work, we executed flow-through electroporation for various duration times (t = 2 ms, 5 ms, and 7 ms), and at various electric field intensities (from 300 to 2200 V/cm) while we utilized different flow rates as well, i. e. 150 μl/min (focused flow) and 600 μl/min (complex flow). To explore the impact of individual electric pulse length and electric pulse number on electroporation results, we designed control channels with straight narrow sections. Cells experience different hydrodynamic forces in straight channels compared to curving channels. Flow pattern and cell focusing were also studied in control channels as well. Also, electroporation on CHO-K1 cells was successfully conducted in control channels. The hydrodynamic forces under the conditions we used do not appear to show substantial impact on transfection efficiency. Advisors/Committee Members: Lu, Chang (committeechair), Ducker, William A. (committee member), Baird, Donald G. (committee member).

Subjects/Keywords: Microfluidics; Electroporation; Particle focusing; CHO-K1 cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hassanisaber, H. (2014). Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/25112

Chicago Manual of Style (16^th Edition):

Hassanisaber, Hamid. “Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.” 2014. Masters Thesis, Virginia Tech. Accessed September 15, 2019. http://hdl.handle.net/10919/25112.

MLA Handbook (7^th Edition):

Hassanisaber, Hamid. “Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels.” 2014. Web. 15 Sep 2019.

Vancouver:

Hassanisaber H. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. [Internet] [Masters thesis]. Virginia Tech; 2014. [cited 2019 Sep 15]. Available from: http://hdl.handle.net/10919/25112.

Council of Science Editors:

Hassanisaber H. Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels. [Masters Thesis]. Virginia Tech; 2014. Available from: http://hdl.handle.net/10919/25112

University of Minnesota

24. Liu, Feng. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.

Degree: M.S.M.E., Mechanical Engineering, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/201723

► Atrial fibrillation currently affects millions of people in the US alone. Focal therapy is an increasingly attractive treatment for atrial fibrillation that avoids the debilitating… (more)

Subjects/Keywords: Atrial fibrillation; Cryoablation; Irreversible Electroporation; Radiofrequency Ablation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, F. (2018). The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/201723

Chicago Manual of Style (16^th Edition):

Liu, Feng. “The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.” 2018. Masters Thesis, University of Minnesota. Accessed September 15, 2019. http://hdl.handle.net/11299/201723.

MLA Handbook (7^th Edition):

Liu, Feng. “The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.” 2018. Web. 15 Sep 2019.

Vancouver:

Liu F. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. [Internet] [Masters thesis]. University of Minnesota; 2018. [cited 2019 Sep 15]. Available from: http://hdl.handle.net/11299/201723.

Council of Science Editors:

Liu F. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. [Masters Thesis]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/201723

Vytautas Magnus University

25. Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.

Degree: Master, Biology, 2008, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

►

Šiame darbe buvo atliekami elektrinio DNR perkėlimo mechanizmų tyrinėjimai in vitro naudojant dviejų tipų impulsus –aukštos įtampos trumpus impulsus (HV) ir žemos įtampos ilgus impulsus… (more)

▼

Šiame darbe buvo atliekami elektrinio DNR perkėlimo mechanizmų tyrinėjimai in vitro naudojant dviejų tipų impulsus –aukštos įtampos trumpus impulsus (HV) ir žemos įtampos ilgus impulsus (LV). DNR elektropernašos eksperimentai buvo atlikti in vitro su Kinijos žiurkėno kiaušidžių CHO ląstelėmis. Eksperimentais buvo siekiama įvertinti HV ir LV impulsų svarbą DNR elektropernašos efektyvumui. Tyrimai parodė, kad DNR elektropernaša vyksta tik po ląstelių elektroporacijos. Naudojant tik LV impulsą (100 V/cm impulso stipris, 100 ms impulso trukmė), kuris ląstelių neelektroporavo, DNR elektropernaša nevyko. LV impulso svarbą DNR elektrotransfekcijos efektyvume, mes tyrėme taikydami LV impulsus 1s po HV impulsų. Kitų autorių in vivo tyrimai parodė, kad jei LV impulsas buvo taikomas po HV impulso, LV smarkiai padidino tranfekcijos efektyvumą. Priešingai nei in vivo eksperimentuose, mūsų in vitro eksperimentai tokios LV impulsų svarbos transfekcijos efektyvumui neparodė. Iš tikrųjų, nei LV impulso amplitudės padidėjimas, nei LV impulsų skaičiaus padidėjimas nepadidino DNR elektrotransfekcijos efektyvumo. Nors, naudojant įvairias HV ir LV impulsų kombinacijas elektrotransfekcijos efektyvumas nebuvo pastovus, jis nesiskyrė žymiai nuo efektyvumo, pasiekto tiktai su HV impulsu.

In this study we setup our experiments to evaluate the roles of high-voltage, short-duration (HV) and low-voltage, long duration (LV) pulses for efficient DNA electrotransfer into cells in vitro. Consistently with others and our previous in vitro and in vivo results, this study confirmed that DNA electrotransfer is possible only if cell permeabilization is achieved. Cell treatment with only one LV pulse (100 V/cm pulse strength, 100 ms pulse duration), that do not permeabilize cells had no effect for DNA electrotransfer. Contribution of LV pulse on overall DNA electrotransfer efficacy we tested by applying LV pulse 1 s following HV pulse. In previous in vivo study such type of experiments revealed that once cell is electropermeabilized, DNA electrotransfer efficacy is governed by the parameters of LV pulse. To further characterize how LV pulses facilitate DNA electrotransfer across the permeabilized membrane we setup this in vitro study. Contrary to in vivo studies, this work revealed that contribution of the LV pulses is negligible. Indeed, neither increase in LV pulse number, nor increase in LV pulse amplitude increased efficacy of DNA electrotransfer. Although, various combinations of HV and LV pulses resulted in slightly variable electrotransfer efficacy, it did not differ significantly from the efficacy achieved with only HV pulse.

Advisors/Committee Members: Šatkauskas, Saulius (Master’s thesis supervisor), Saulis, Gintautas (Master’s thesis reviewer).

Subjects/Keywords: Elektroporacija; Elektrogenoterapija; Elektropernaša; Electroporation; Electrogeneterapy; Electrotransfer

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kačkauskaitė, Dovilė. (2008). Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. (Masters Thesis). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Kačkauskaitė, Dovilė. “Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.” 2008. Masters Thesis, Vytautas Magnus University. Accessed September 15, 2019. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Kačkauskaitė, Dovilė. “Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro.” 2008. Web. 15 Sep 2019.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. [Internet] [Masters thesis]. Vytautas Magnus University; 2008. [cited 2019 Sep 15]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Kačkauskaitė, Dovilė. Ląstelių elektroporacija ir DNR elektropernaša į ląsteles in vitro. [Masters Thesis]. Vytautas Magnus University; 2008. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2008~D_20080627_112301-73489 ;

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vytautas Magnus University

26. Čepurnienė, Karolina. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.

Degree: PhD, Biochemistry, 2010, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

►

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency… (more)

▼

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency into the cells both in suspension and in monolayer. In order to deliver various combinations of HV and LV electric pulses an original electroporator has been developed. Contrary to many studies in vivo and several studies in vitro we have shown that LV pulses do not increase number of transfected cells both in suspension and in monolayer, when 100 µg/ml plasmid concentration was used. Analyzing DNA electrotransfer into CHO cells grown in monolayer we observed, that some of these cell fuse forming multinuclear cell structures. Examining this effect we have shown that cell fusion depends on parameters of electric pulses and more intriguingly on cells type. We have shown that the extent of electrofusion in B16F10 cells was significantly larger than other types of cells. Thus these results allowed us to hypothesize that cells from metastatic tumours have greater propensity for cell electrofusion. This effect has been investigated in more detail. Using GFP and luciferase coding plasmids at various concentrations (1, 10 and 100 µg/ml) we were the first to demonstrate that the increase in transfection efficiency by LV pulses is evident only at reduced plasmid concentration. This increase, in comparison to transfection induced using HV pulse alone, was more pronounced with more reduced concentrations of the plasmid.... [to full text]

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Šių tyrimų rezultatai parodė, kad mažų molekulių, tokių kaip propidžio jodidas ir didelių molekulių, tokių, kaip plazmidinė DNR, elektropernašos į ląsteles in vitro mechanizmai yra skirtingi. Skirtingai nuo mažų molekulių patekimo, plazmidinės DNR elektropernaša galima tik tuo atveju jei plazmidė ląstelių suspensijoje yra AA impulso taikymo metu. Naudodami AA ir ŽA impulsų kombinacijas, savo tyrimais pirmieji parodėme, kad suspensijoje ir ląstelėms augančioms monosluoksniu, ŽA impulsai po ląstelių elektroporacijos AA impulsu, kai naudota 100 µg/ml plazmidės koncentracija, transfekuotų ląstelių skaičiui įtakos neturi. Tiriant molekulių patekimą į ląsteles paveikus jas elektriniais laukais pastebėjome, kad kai kurios ląstelės augančios monosluoksniu gali susilieti. Tyrimais nustatėme, kad ląstelių susiliejimas priklauso nuo elektrinio lauko stiprio ir ląstelių linijos. Mūsų in vitro tyrimų rezultatai parodė kad daugiausia ląstelių po elektroporacijos susilieja B16F10 linijoje ląstelėse. Tai patvirtino ir sustiprino ankstesnes hipotezes, kad iš metastazuojančių navikų, ląstelių elektrosusiliejimo kiekis žymiai didesnis, nei normalių ląstelių. Vertinant ŽA impulso įtaką perneštos DNR kiekiui, nustatėme, kad ŽA impulso įtaka stebima kuomet ląstelių suspensijoje plazmidės koncentracija yra sumažinta (10 µg/ml, 1 µg/ml).... [toliau žr. visą tekstą]

Advisors/Committee Members: Šatkauskas, Saulius (Doctoral dissertation supervisor), Skeberdis, Vytenis Arvydas (Doctoral dissertation opponent), Kirvelienė, Vida (Doctoral dissertation opponent), Paulauskas, Algimantas (Doctoral dissertation committee chair), Mildažienė, Vida (Doctoral dissertation committee member), Rukšėnas, Osvaldas (Doctoral dissertation committee member), Meškys, Rolandas (Doctoral dissertation committee member), Jurevičius, Jonas (Doctoral dissertation committee member).

Subjects/Keywords: Electroporation; Electrotransfection; DNA; Electroporacija; Elektrotransfekcija; DNR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Čepurnienė, K. (2010). Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. (Doctoral Dissertation). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

Chicago Manual of Style (16^th Edition):

Čepurnienė, Karolina. “Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.” 2010. Doctoral Dissertation, Vytautas Magnus University. Accessed September 15, 2019. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;.

MLA Handbook (7^th Edition):

Čepurnienė, Karolina. “Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses.” 2010. Web. 15 Sep 2019.

Vancouver:

Čepurnienė K. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. [Internet] [Doctoral dissertation]. Vytautas Magnus University; 2010. [cited 2019 Sep 15]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;.

Council of Science Editors:

Čepurnienė K. Investigation of DNA electrotransfer into cells in vitro using high and low voltage electric pulses. [Doctoral Dissertation]. Vytautas Magnus University; 2010. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085343-41592 ;

Vytautas Magnus University

27. Čepurnienė, Karolina. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.

Degree: Dissertation, Biochemistry, 2010, Vytautas Magnus University

URL: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

►

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Tyrimų rezultatai parodė, kad… (more)

▼

Šiame darbe buvo tirta DNR elektropernašos efektyvumas į Kininio žiurkėno kiaušidžių ląsteles naudojant aukštos (AA) ir žemos (ŽA) amplitudės elektrinius impulsus. Tyrimų rezultatai parodė, kad mažų molekulių, tokių kaip propidžio jodidas ir didelių molekulių, tokių, kaip plazmidinė DNR, elektropernašos į ląsteles in vitro mechanizmai yra skirtingi. Skirtingai nuo mažų molekulių patekimo, plazmidinės DNR elektropernaša galima tik tuo atveju jei plazmidė ląstelių suspensijoje yra AA impulso taikymo metu. Naudodami AA ir ŽA impulsų kombinacijas, savo tyrimais pirmieji parodėme, kad suspensijoje ir ląstelėms augančioms monosluoksniu, ŽA impulsai po ląstelių elektroporacijos AA impulsu, kai naudota 100 µg/ml plazmidės koncentracija, transfekuotų ląstelių skaičiui įtakos neturi. Tiriant molekulių patekimą į ląsteles paveikus jas elektriniais laukais pastebėjome, kad kai kurios ląstelės augančios monosluoksniu gali susilieti. Tyrimais nustatėme, kad ląstelių susiliejimas priklauso nuo elektrinio lauko stiprio ir ląstelių linijos. Mūsų in vitro tyrimų rezultatai parodė kad daugiausia ląstelių po elektroporacijos susilieja B16F10 linijoje ląstelėse. Tai patvirtino ir sustiprino ankstesnes hipotezes, kad iš metastazuojančių navikų, ląstelių elektrosusiliejimo kiekis žymiai didesnis, nei normalių ląstelių. Vertinant ŽA impulso įtaką perneštos DNR kiekiui, nustatėme, kad ŽA impulso įtaka stebima kuomet ląstelių suspensijoje plazmidės koncentracija yra sumažinta (10 µg/ml, 1 µg/ml).... [toliau žr. visą tekstą]

In the present work we analyzed short duration, high voltage (HV) and long duration, low voltage (LV) and their combinations influence for DNA electrotransfer efficiency into the cells both in suspension and in monolayer. In order to deliver various combinations of HV and LV electric pulses an original electroporator has been developed. Contrary to many studies in vivo and several studies in vitro we have shown that LV pulses do not increase number of transfected cells both in suspension and in monolayer, when 100 µg/ml plasmid concentration was used. Analyzing DNA electrotransfer into CHO cells grown in monolayer we observed, that some of these cell fuse forming multinuclear cell structures. Examining this effect we have shown that cell fusion depends on parameters of electric pulses and more intriguingly on cells type. We have shown that the extent of electrofusion in B16F10 cells was significantly larger than other types of cells. Thus these results allowed us to hypothesize that cells from metastatic tumours have greater propensity for cell electrofusion. This effect has been investigated in more detail. Using GFP and luciferase coding plasmids at various concentrations (1, 10 and 100 µg/ml) we were the first to demonstrate that the increase in transfection efficiency by LV pulses is evident only at reduced plasmid concentration. This increase, in comparison to transfection induced using HV pulse alone, was more pronounced with more reduced concentrations of the plasmid.... [to full text]

Advisors/Committee Members: Šatkauskas, Saulius (Doctoral dissertation supervisor), Skeberdis, Vytenis Arvydas (Doctoral dissertation opponent), Kirvelienė, Vida (Doctoral dissertation opponent), Paulauskas, Algimantas (Doctoral dissertation committee chair), Mildažienė, Vida (Doctoral dissertation committee member), Rukšėnas, Osvaldas (Doctoral dissertation committee member), Meškys, Rolandas (Doctoral dissertation committee member), Jurevičius, Jonas (Doctoral dissertation committee member).

Subjects/Keywords: Elektroporacija; Elektrotransfekcija; DNR; Electroporation; Electrotransfection; DNA

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Čepurnienė, K. (2010). DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. (Doctoral Dissertation). Vytautas Magnus University. Retrieved from http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

Chicago Manual of Style (16^th Edition):

Čepurnienė, Karolina. “DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.” 2010. Doctoral Dissertation, Vytautas Magnus University. Accessed September 15, 2019. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;.

MLA Handbook (7^th Edition):

Čepurnienė, Karolina. “DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus.” 2010. Web. 15 Sep 2019.

Vancouver:

Čepurnienė K. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. [Internet] [Doctoral dissertation]. Vytautas Magnus University; 2010. [cited 2019 Sep 15]. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;.

Council of Science Editors:

Čepurnienė K. DNR elektropernašos tyrimai į ląsteles in vitro naudojant aukštos ir žemos amplitudės elektrinius impulsus. [Doctoral Dissertation]. Vytautas Magnus University; 2010. Available from: http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101021_085554-66760 ;

North Carolina State University

28. Becker, Sid. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.

Degree: PhD, Mechanical Engineering, 2007, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/4991

► Electroporation is an approach used to enhance the transport of large molecules to the cell cytosol in which a targeted tissue region is exposed to… (more)

Subjects/Keywords: skin; electroporation; numerical

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Becker, S. (2007). In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/4991

Chicago Manual of Style (16^th Edition):

Becker, Sid. “In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.” 2007. Doctoral Dissertation, North Carolina State University. Accessed September 15, 2019. http://www.lib.ncsu.edu/resolver/1840.16/4991.

MLA Handbook (7^th Edition):

Becker, Sid. “In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport.” 2007. Web. 15 Sep 2019.

Vancouver:

Becker S. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. [Internet] [Doctoral dissertation]. North Carolina State University; 2007. [cited 2019 Sep 15]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/4991.

Council of Science Editors:

Becker S. In Vivo Electroporation of Skin and Biological Tissue: Theoretical model development and Numerical Investigation of Associated Thermo-Electrical and Structural Responses and Enhanced Mass Transport. [Doctoral Dissertation]. North Carolina State University; 2007. Available from: http://www.lib.ncsu.edu/resolver/1840.16/4991

University of Notre Dame

29. Ashley Nicole Swanson. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.

Degree: MS, Biological Sciences, 2016, University of Notre Dame

URL: https://curate.nd.edu/show/ff365427t4d

► Mycobacterium avium is an environmental bacteria that can cause disease in people with pre-existing lung conditions or who are immunocompromised. Mycobacteria are well known… (more)

Subjects/Keywords: Glycopeptidolipids; RNA Sequencing; Mycobacterium avium; Transformation; Electroporation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Swanson, A. N. (2016). Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. (Masters Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/ff365427t4d

Chicago Manual of Style (16^th Edition):

Swanson, Ashley Nicole. “Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.” 2016. Masters Thesis, University of Notre Dame. Accessed September 15, 2019. https://curate.nd.edu/show/ff365427t4d.

MLA Handbook (7^th Edition):

Swanson, Ashley Nicole. “Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>.” 2016. Web. 15 Sep 2019.

Vancouver:

Swanson AN. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. [Internet] [Masters thesis]. University of Notre Dame; 2016. [cited 2019 Sep 15]. Available from: https://curate.nd.edu/show/ff365427t4d.

Council of Science Editors:

Swanson AN. Using Molecular Techniques to Define the Role of Glycopeptidolipids in Mycobacterium avium Pathogenesis</h1>. [Masters Thesis]. University of Notre Dame; 2016. Available from: https://curate.nd.edu/show/ff365427t4d

Brigham Young University

30. Wilson, Aubrey Marie Mueller. Transgene Delivery via Microelectromechanical Systems.

Degree: MS, 2012, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

► The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene… (more)

▼ The invention of pronuclear microinjection initiated the field of transgenic research. Over 30 years later microinjection remains the most straight-forward and most commonly used transgene delivery option. In this work we address the current progress of microelectromechanical systems (MEMS) used as transgenic delivery mechanisms. The nanoinjector is a specially designed MEMS device which uses electrostatic charge to manipulate transgene molecules. The process of nanoinjection was designed as an alternative to microinjection which causes less damage to developing embryos, improves embryo survival, birth rates, and overall efficiency of injections. In vivo testing of nanoinjection demonstrates it is both safe and effective. Additionally nanoinjection has the potential to make transgenesis via yeast artificial chromosomes more practical as the nanoinjector may prevent shearing of the YAC molecules. A second nanoinjection protocol termed intracellular electroporetic nanoinjcetion (IEN) was designed to allow for cytoplasmic injections. Cytoplasmic injections are faster and easier than pronuclear injection and do not require the pronuclei to be visible; yet previous attempts to develop cytoplasmic injection have met with limited success. In IEN injections the nanoinjector is used to place transgenic molecules in the cytoplasm. The transgenes are then propelled through the cytoplasm and electroporated into the pronucleus using electrical pulses. Electroporation of whole embryos has not resulted in transgenic animals, but the MEMS device allows localized electroporation to occur within the cytoplasm, giving transgene access to the pronucleus before degradation can occur. In this report we describe the principles which allow for localized electroporation of the pronuclei including: the location of the pronuclei between 21-28 hours post-hCG treatment, modeling data predicting the voltages needed for localized electroporation of pronuclei, and data on the movement of transgenic DNA based on the voltages delivered by IEN. We further report results of an IEN versus microinjection comparative study in which IEN produced transgenic pups with viability, transgene integration, and expression rates statistically comparable to microinjection. The ability to perform injections without visualizing or puncturing the pronuclei will widely benefit transgenic research, and will be particularly advantageous for the production of transgenic animals with embryos exhibiting reduced pronuclear visibility.

Subjects/Keywords: nanoinjection; microinjection; transgenic; electroporation; DNA transfer; Microbiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wilson, A. M. M. (2012). Transgene Delivery via Microelectromechanical Systems. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

Chicago Manual of Style (16^th Edition):

Wilson, Aubrey Marie Mueller. “Transgene Delivery via Microelectromechanical Systems.” 2012. Masters Thesis, Brigham Young University. Accessed September 15, 2019. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd.

MLA Handbook (7^th Edition):

Wilson, Aubrey Marie Mueller. “Transgene Delivery via Microelectromechanical Systems.” 2012. Web. 15 Sep 2019.

Vancouver:

Wilson AMM. Transgene Delivery via Microelectromechanical Systems. [Internet] [Masters thesis]. Brigham Young University; 2012. [cited 2019 Sep 15]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd.

Council of Science Editors:

Wilson AMM. Transgene Delivery via Microelectromechanical Systems. [Masters Thesis]. Brigham Young University; 2012. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4935&context=etd

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations