subject:(dynamic speckle)

Brno University of Technology

1. Pavloň, Martin. Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/65063

► This diploma thesis deals with the theoretical description of interference methods used to measure vibrations, examines main parasitic effects and analyses its contribution to achievable… (more)

Subjects/Keywords: Laserový dopplerovský vibrometr; bezkontaktní měření vibrací; parazitní vlivy; vlastní šum; koherenční zrnitost; dynamické parametry; PDV 100; OFV 505; OFV-5000; CLV 2534.; Laser Doppler vibrometer; non-contact vibration measurement; parasitic effects; self noise; speckle; dynamic parameters; PDV 100; OFV 505; OFV 5000; CLV 2534.

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APA (6^th Edition):

Pavloň, M. (2019). Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/65063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pavloň, Martin. “Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers.” 2019. Thesis, Brno University of Technology. Accessed January 19, 2021. http://hdl.handle.net/11012/65063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pavloň, Martin. “Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers.” 2019. Web. 19 Jan 2021.

Vancouver:

Pavloň M. Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11012/65063.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pavloň M. Analýza dynamických parametrů laserových vibrometrů: Analysis of dynamic parameters of laser vibrometers. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/65063

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Zahnd, Guillaume. Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking.

Degree: Docteur es, Acoustique et traitement de signal/image, 2012, INSA Lyon

URL: http://www.theses.fr/2012ISAL0136

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Ce travail de thèse est axé sur le domaine du traitement d'images biomédicales. L'objectif de notre étude est l'estimation des paramètres traduisant les propriétés mécaniques… (more)

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Ce travail de thèse est axé sur le domaine du traitement d'images biomédicales. L'objectif de notre étude est l'estimation des paramètres traduisant les propriétés mécaniques de l'artère carotide in vivo en imagerie échographique, dans une optique de détection précoce de la pathologie cardiovasculaire. L'analyse du mouvement longitudinal des tissus de la paroi artérielle, i.e. dans la même direction que le flux sanguin, représente la motivation majeure de ce travail. Les trois contributions principales proposées dans ce travail sont i) le développement d'un cadre méthodologique original et semi-automatique, dédié à la segmentation et à l'estimation du mouvement de la paroi artérielle dans des séquences in vivo d'images ultrasonores mode-B, ii) la description d'un protocole de génération d'une référence, faisant intervenir les opérations manuelles de plusieurs experts, dans le but de quantifier la précision des résultats de notre méthode malgré l'absence de vérité terrain inhérente à la modalité échographique, et iii) l'évaluation clinique de l'association entre les paramètres mécaniques et dynamiques de la paroi carotidienne et les facteurs de risque cardiovasculaire dans le cadre de la détection précoce de l'athérosclérose. Nous proposons une méthode semi-automatique, basée sur une approche conjointe de segmentation des contours de la paroi et d'estimation du mouvement des tissus. L'extraction de la position des interfaces est réalisée via une approche spécifique à la structure morphologique de la carotide, basée sur une stratégie de programmation dynamique exploitant un filtrage adapté. L'estimation du mouvement est réalisée via une méthode robuste de mise en correspondance de blocs (block matching), basée sur la connaissance du déplacement a priori ainsi que sur la mise à jour temporelle du bloc de référence par un filtre de Kalman spécifique. La précision de notre méthode, évaluée in vivo, correspond au même ordre de grandeur que celle résultant des opérations manuelles réalisées par des experts, et reste sensiblement meilleure que celle obtenue avec deux autres méthodes traditionnelles (i.e. une implémentation classique de la technique de block matching et le logiciel commercial Velocity Vector Imaging). Nous présentons également quatre études cliniques réalisées en milieu hospitalier, où nous évaluons l'association entre le mouvement longitudinal et les facteurs de risque cardiovasculaire. Nous suggérons que le mouvement longitudinal, qui représente un marqueur de risque émergent et encore peu étudié, constitue un indice pertinent et complémentaire aux marqueurs traditionnels dans la caractérisation de la physiopathologie artérielle, reflète le niveau de risque cardiovasculaire global, et pourrait être bien adapté à la détection précoce de l'athérosclérose.

This thesis is focused on the domain of bio-medical image processing. The aim of our study is to assess in vivo the parameters traducing the mechanical properties of the carotid artery in ultrasound imaging, for early detection of cardiovascular diseases.…

Advisors/Committee Members: Vray, Didier (thesis director).

Subjects/Keywords: Imagerie médicale; Imagerie ultrasonore; Traitement d'image - Digital techniques; Echographie; Echographie endovasculaire; Echographie mode B; Estimation du mouvement; Filtre de Kalman; Segmentation d'images; Segmentation de contour; Déctection de gradient; Filtre adapté; Programmation dynamique; Propagation de front; Artère; Athérosclérose; Pathologie cardiovasculaire; Medical Imaging; Ultrasound Imaging; Image analysis; Echography; Movement Estimation; Block matching; Speckle tracking; Kalman Filter; Image segmentation; Filtering algorithm; Dynamic programming; Artery; Atheroscerosis; 616.075 430 72

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zahnd, G. (2012). Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking. (Doctoral Dissertation). INSA Lyon. Retrieved from http://www.theses.fr/2012ISAL0136

Chicago Manual of Style (16^th Edition):

Zahnd, Guillaume. “Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking.” 2012. Doctoral Dissertation, INSA Lyon. Accessed January 19, 2021. http://www.theses.fr/2012ISAL0136.

MLA Handbook (7^th Edition):

Zahnd, Guillaume. “Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking.” 2012. Web. 19 Jan 2021.

Vancouver:

Zahnd G. Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking. [Internet] [Doctoral dissertation]. INSA Lyon; 2012. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2012ISAL0136.

Council of Science Editors:

Zahnd G. Estimation du mouvement bi-dimensionnel de la paroi artérielle en imagerie ultrasonore par une approche conjointe de segmentation et de speckle tracking : Estimation of the bi-dimensional motion of the arterial wall in ultrasound imaging with a combined approach of segmentation and speckle tracking. [Doctoral Dissertation]. INSA Lyon; 2012. Available from: http://www.theses.fr/2012ISAL0136

3. Young, Anthony M. Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow.

Degree: MS, Physics, 2018, Miami University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362

► This thesis presents data from a series of experiments that investigate the ability of laser speckle contrast imaging (LSCI) to sense changes in flow in… (more)

Subjects/Keywords: Biomedical Research; Biophysics; Optics; Medical Imaging; Physics; laser speckle; laser speckle contrast imaging; flow imaging; dynamic light scattering; coherence imaging; biomedical imaging; blood flow measurement; flow phantom

…of speckle patterns and that which relates dynamic speckle to the velocity of scatterers. A… …21 Figure 10. Schematic of the imaging system used to collect speckle images. Flip mirrors… …22 Figure 11. The raw speckle image (Fig. 11a) and the LSCI flow map of 𝜏c-1… …16b used to collect speckle images. D: Ground diffuser; BE: Beam expander; P1: Illumination… …29 Figure 17. An image of the flow phantom (Fig. 17a) and captured raw speckle…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Young, A. M. (2018). Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow. (Masters Thesis). Miami University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362

Chicago Manual of Style (16^th Edition):

Young, Anthony M. “Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow.” 2018. Masters Thesis, Miami University. Accessed January 19, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362.

MLA Handbook (7^th Edition):

Young, Anthony M. “Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow.” 2018. Web. 19 Jan 2021.

Vancouver:

Young AM. Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow. [Internet] [Masters thesis]. Miami University; 2018. [cited 2021 Jan 19]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362.

Council of Science Editors:

Young AM. Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow. [Masters Thesis]. Miami University; 2018. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362

4. Wells-Gray, Elaine Marie. Development and application of dynamic light scattering methods for charcaterizing reaction kinetics.

Degree: PhD, 2011, Oregon Health Sciences University

URL: doi:10.6083/M4K64G2C ; http://digitalcommons.ohsu.edu/etd/545

Subjects/Keywords: Kinetics; Laser speckle; Photopolymerization; Dental materials; Dynamic Light Scattering; Kinetics; Dental Materials; Lasers; Polymerization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wells-Gray, E. M. (2011). Development and application of dynamic light scattering methods for charcaterizing reaction kinetics. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4K64G2C ; http://digitalcommons.ohsu.edu/etd/545

Chicago Manual of Style (16^th Edition):

Wells-Gray, Elaine Marie. “Development and application of dynamic light scattering methods for charcaterizing reaction kinetics.” 2011. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021. doi:10.6083/M4K64G2C ; http://digitalcommons.ohsu.edu/etd/545.

MLA Handbook (7^th Edition):

Wells-Gray, Elaine Marie. “Development and application of dynamic light scattering methods for charcaterizing reaction kinetics.” 2011. Web. 19 Jan 2021.

Vancouver:

Wells-Gray EM. Development and application of dynamic light scattering methods for charcaterizing reaction kinetics. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 2011. [cited 2021 Jan 19]. Available from: doi:10.6083/M4K64G2C ; http://digitalcommons.ohsu.edu/etd/545.

Council of Science Editors:

Wells-Gray EM. Development and application of dynamic light scattering methods for charcaterizing reaction kinetics. [Doctoral Dissertation]. Oregon Health Sciences University; 2011. Available from: doi:10.6083/M4K64G2C ; http://digitalcommons.ohsu.edu/etd/545

5. Dupont, Jan. Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation.

Degree: Docteur es, Electromagnétisme et systèmes de haute fréquence, 2017, Toulouse, ISAE

URL: http://www.theses.fr/2017ESAE0002

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Lors de la diffusion d’une onde électromagnétique sur une surface de rugosité aléatoire ou dans un volume, un champ de speckle, dont les caractéristiques dépendent… (more)

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Lors de la diffusion d’une onde électromagnétique sur une surface de rugosité aléatoire ou dans un volume, un champ de speckle, dont les caractéristiques dépendent du diffuseur considéré, se forme. Les diffusions au sein de matériaux impactent l’état de polarisation d’une lumière incidente. Ainsi, la polarisation est un paramètre sensible pour la caractérisation et l’étude de matériaux. Une technique de polarimétrie résolue spatialement, permettant une mesure de précision en champs de speckle, est proposée. Cette technique est utilisée pour étudier l’impact de différents paramètres d’imagerie sur la mesure polarimétrique, notamment les phénomènes de dépolarisation des champs dus au mode d’observation. Un modèle de simulation de champs de speckle polarisés, validé par comparaison avec l'expérimentation pour différents régimes de diffusions, est développé. Par ailleurs, les propriétés dynamiques de l’échantillon peuvent être mesurées par une analyse de contraste du speckle qu’il diffuse. Un dispositif d’imagerie de micro-vascularisation par mesure de contraste de speckle dynamique polarisé est optimisé, puis appliqué à l’étude in-vivo de l’angiogenèse tumorale du mélanome murin, ainsi que l’évolution de la vascularisation après traitement des tumeurs par électrochimiothérapie. Le potentiel de la technique pour la détection et l'étude du mélanome murin, sans contact ni marqueur, est démontré, avec comme perspective la détection et l'étude du mélanome humain, dont l'efficacité reste à être caractérisée pour une utilisation en imagerie biomédicale.

When an electromagnetic wave is scattered by a rough surface or in a volume, a speckle field is observed, with characterlstlcs depending on the consldered scatterer. Multiple scattering in samples immpact the State of polarizatlon of an incident light. Thus, polarization Is a sensitive parameter for material characterization and study. A spatially resolved polarlmetry method, allowing accurate measurements in speckle fields is proposed. That method is used to study the Impact of various parameters on polarimétrie measurements, especially the depolarization phenomenon due to the observation setup. A polarlzed speskle simulation model is proposed, validated by comparison with expérimentation for various scattering régime. Besides, dynamlc properties of samples can be measured by an analysis of the scattered speckle contrast. A method allowing microvascularization imaging based on dynamic polarized light scattering imaging is optlmlzed, then applied to in-vivo study of the tumor angiogenesis occuring on murine melanoma, as well as the vascularization évolution after a treatment called electrochemotherapy. Potentlal of the method for non invasive détection and study of the murine melanoma is demonstrated, its efflciency on human melanoma for biomédical applications remaning to be characterized.

Advisors/Committee Members: Golzio, Muriel (thesis director), Orlik, Xavier (thesis director).

Subjects/Keywords: Imagerie polarimétrique; Speckle; Polarimétrie de speckle; Dépolarlsation spatiale; Imagerie biomédicale; Imagerie par contraste de speckle dynamique; Mélanome; Polarimétrie imaging; Speckle; Speckle polarlmetry; Spatial depolarization; Biomédical Imaging; Dynamic speckle contrast imaging; Melanoma; 621

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dupont, J. (2017). Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation. (Doctoral Dissertation). Toulouse, ISAE. Retrieved from http://www.theses.fr/2017ESAE0002

Chicago Manual of Style (16^th Edition):

Dupont, Jan. “Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation.” 2017. Doctoral Dissertation, Toulouse, ISAE. Accessed January 19, 2021. http://www.theses.fr/2017ESAE0002.

MLA Handbook (7^th Edition):

Dupont, Jan. “Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation.” 2017. Web. 19 Jan 2021.

Vancouver:

Dupont J. Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation. [Internet] [Doctoral dissertation]. Toulouse, ISAE; 2017. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2017ESAE0002.

Council of Science Editors:

Dupont J. Imagerie polarimétrique de speckle statique pour l’étude de matériaux et dynamique pour la détection de micro-vascularisation tumorale : Static speckle polarimetric imaging for material study, and dynamic for the detection of tumoral micro-vascularlzation. [Doctoral Dissertation]. Toulouse, ISAE; 2017. Available from: http://www.theses.fr/2017ESAE0002

6. Rafael Rodrigues Cardoso. Análise do speckle laser dinâmico: uma releitura.

Degree: 2011, UNIVERSIDADE FEDERAL DE LAVRAS

URL: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=3667

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A análise de imagem por si mesma se apresenta como um poderoso instrumento aplicado ao monitoramento a toda espécie de fenômenos biológicos. O desenvolvimento de… (more)

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A análise de imagem por si mesma se apresenta como um poderoso instrumento aplicado ao monitoramento a toda espécie de fenômenos biológicos. O desenvolvimento de diversos tipos de abordagens ópticas com o objetivo de se obter viabilidade na aquisição e análise de imagens tem sido um dos principais esforços nessa área de aplicação. Uma consequência desse esforço é a adoção da técnica do biospeckle laser como uma alternativa em potencial para se alcançar uma metrologia óptica. O biospeckle ou speckle laser dinâmico é um fenômeno formado quando um processo dinâmico ocorre em um material que é iluminado pelo laser. Esse fenômeno contém informações a respeito da atividade presente nesse material, seja biológico ou não. Por ser uma técnica não invasiva, não destrutiva e de custo baixo, o biospeckle laser (BSL) tem sido uma boa ferramenta no monitoramento de propriedades biológicas. Nesse sentido, a aplicação em instrumentação óptica tem crescido ao longo dos anos, especialmente nas áreas da biologia, medicina e agricultura. A análise do speckle dinâmico é feita usando técnicas de processamento digital de imagem e análises estatísticas. Contudo, essa análise tem sido um desafio para os especialistas da área devido à complexidade envolvida na interação da luz laser e o material em estudo. Assim, é necessário desenvolver e aperfeiçoar métodos e protocolos que assegurem maior eficiência em medir e monitorar o processo dinâmico no material em estudo. A alta sensibilidade do BSL e a variabilidade de materiais biológicos associados com o grande número de variáveis envolvidas na formação do padrão de speckle têm trazido grandes desafios na busca por técnicas de análise mais seguras e robustas. Esse estudo tem como objetivo desenvolver e refinar metodologias de análise do BSL e criar protocolos para diferentes tipos de análises. Em específico, são apresentados: um protocolo para separar diferentes tecidos em um mesmo material através da assinatura espectral de cada tecido e por meio da associação de resultados gráficos e numéricos do BSL; um procedimento para avaliar a qualidade de imagem durante a fase de iluminação, baseando-se na saturação, áreas escuras, formação do padrão de speckle e na homogeneidade da imagem; melhorias e novas abordagens na técnica do Momento de Inércia, através de uma nova normalização da matriz de ocorrência e usando quantificação contínua por meio da redução da informação temporal; e, uma nova técnica gráfica baseado no desvio padrão de cada pixel em relação ao tempo. Os resultados mostram a viabilidade das metodologias descritas que alcançaram maior confiabilidade e coerência na análise do BSL.

The image analysis presents itself as a powerful instrument applied to all sort of biological phenomena monitoring. The development of many optical approaches to carry out a feasible image assembling and analysis to different demands has been the main effort in this application area. A consequence of that effort is the adoption of the biospeckle laser technique as a potential alternative to pursue the optical…

Advisors/Committee Members: Thelma Sáfadi, Roberto Alves Braga Júnior, Giovanni Francisco Rabelo.

Subjects/Keywords: Speckle dinâmico; novas técnicas de análise; processamento de sinais; ENGENHARIA AGRICOLA; dynamic speckle; new analysis technique; signal processing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cardoso, R. R. (2011). Análise do speckle laser dinâmico: uma releitura. (Thesis). UNIVERSIDADE FEDERAL DE LAVRAS. Retrieved from http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=3667

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cardoso, Rafael Rodrigues. “Análise do speckle laser dinâmico: uma releitura.” 2011. Thesis, UNIVERSIDADE FEDERAL DE LAVRAS. Accessed January 19, 2021. http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=3667.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cardoso, Rafael Rodrigues. “Análise do speckle laser dinâmico: uma releitura.” 2011. Web. 19 Jan 2021.

Vancouver:

Cardoso RR. Análise do speckle laser dinâmico: uma releitura. [Internet] [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2011. [cited 2021 Jan 19]. Available from: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=3667.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cardoso RR. Análise do speckle laser dinâmico: uma releitura. [Thesis]. UNIVERSIDADE FEDERAL DE LAVRAS; 2011. Available from: http://bdtd.ufla.br//tde_busca/arquivo.php?codArquivo=3667

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Brunel, Benjamin. Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering.

Degree: Docteur es, Physique pour les sciences du vivant, 2018, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2018GREAY047

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Lorsqu'une tumeur grossit, elle exerce une pression sur les tissus environnants et est comprimée en retour. Des expériences sur un modèle de tumeur in vitro,… (more)

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Lorsqu'une tumeur grossit, elle exerce une pression sur les tissus environnants et est comprimée en retour. Des expériences sur un modèle de tumeur in vitro, appelé sphéroïde, ont montré que cette pression influence largement le devenir du tissu cancéreux, notamment en freinant sa croissance, mais aussi en le rendant plus invasif. Pour mieux comprendre ce dernier effet, nous avons cherché à étudier le comportement migratoire des cellules à l'intérieur d'un sphéroïde sous pression. Observer l'intérieur d'un sphéroïde pose cependant un problème technique car les méthodes usuelles d'imagerie ne sont pas utilisables dans des tissus épais (> 100 μm). L'imagerie classique étant limitée en profondeur à cause de la diffusion de la lumière, nous nous sommes tournés vers une méthode qui utilise justement celle-ci : la diffusion dynamique de lumière ou DLS (Dynamic Light Scattering). Nous avons développé son application à la migration cellulaire, afin d'obtenir la distribution des déplacements relatifs des cellules au cours du temps. Cette mesure est faite sans utiliser de marqueurs spécifiques et est applicable à des sphéroïdes allant jusqu'à 400 μm de diamètre. Nous avons ainsi mis en évidence une organisation radiale du sphéroïde en termes de mobilité, avec des cellules rapides en surface et plus lentes au centre. Nous avons aussi montré qu'en appliquant une contrainte au sphéroïde, la vitesse moyenne diminue jusqu'à être réduite de moitié pour des pressions supérieures à 15kPa. Une autre équipe a mesuré par ailleurs une augmentation de la vitesse des cellules en surface suite à une compression, ce qui indique que l'organisation radiale se retrouve dans la réponse à la pression. Nous avons montré que cette sensibilité à la pression est une propriété qui émerge de l'organisation 3D du tissu, dans laquelle la matrice extracellulaire joue un rôle primordial. Enfin, pour explorer les possibilités qu'offre notre technique, nous l'avons appliquée à une autre question : comment la migration des macrophages est-elle affectée par les signaux provenant de cellules apoptotiques ? Les résultats ont montré que les cellules apoptotiques précoces augmentent la vitesse des macrophages tandis que les cellules apoptotiques tardives la réduisent. D'un cas à l'autre, la longueur de persistance du mouvement est conservée.

As a tumor grows, it exerts a mechanical pressure on its surrounding tissue and is compressed back as a reaction. Recent experiments on an in vitro tumor model, called spheroid, have shown that this pressure is crucial for the fate of the cancerous tissue. In particular, the pressure slows down its growth, but makes it more invasive. To further understand the latter effect, we decided to study the migration of cells inside spheroids under pressure. However, imaging the inside of a spheroid is technically challenging as usual microscopy methods do not work on thick tissues (> 100 μm). Standard imaging methods are limited in terms of depth penetration because of light scattering. For this reason, we decided to take advantage…

Advisors/Committee Members: Cappello, Giovanni (thesis director).

Subjects/Keywords: Diffusion dynamique de lumière; Motilité cellulaire; Agrégats multicellulaires; Cancer; Speckle; Mécanique des tissus; Dynamic Light Scattering; Cell motility; Multicellular aggregates; Cancer; Speckle; Mechanical properties of tissues; 530

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brunel, B. (2018). Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2018GREAY047

Chicago Manual of Style (16^th Edition):

Brunel, Benjamin. “Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering.” 2018. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed January 19, 2021. http://www.theses.fr/2018GREAY047.

MLA Handbook (7^th Edition):

Brunel, Benjamin. “Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering.” 2018. Web. 19 Jan 2021.

Vancouver:

Brunel B. Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2018. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2018GREAY047.

Council of Science Editors:

Brunel B. Mesure des déplacements cellulaires dans les tissus non transparents : une application de la diffusion dynamique de la lumière : Measuring cell displacements inside non-transparent tissues : an application of dynamic light scattering. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2018. Available from: http://www.theses.fr/2018GREAY047

8. Ali, Nabil. RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales.

Degree: Docteur es, Mécanique des fluides, procédés, énergétique, 2016, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2016GREAI013

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Ce travail de thèse cherche à mieux caractériser le comportement des matériaux hétérogènes sous cisaillement avec une approche multi-échelles (macro-méso-microscopique).Cela est rendu possible en développant… (more)

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Ce travail de thèse cherche à mieux caractériser le comportement des matériaux hétérogènes sous cisaillement avec une approche multi-échelles (macro-méso-microscopique).Cela est rendu possible en développant un montage innovant qui couple un rhéomètre à un système d’imagerie de speckle résolue spatialement et temporellement (RheoSpeckle). Nous montrons la validation de notre expérience en l’appliquant sur deux matériaux parfaits : un solide et un liquide. Sur le solide, on mesure le champ de déplacement sur les images de speckle avec une résolution meilleure que 1 µm. Puis on prouve l’élasticité du matériau à l’échelle microscopique. Sur le liquide, la taille des nanoparticules est déterminée avec un excellent accord avec la spécification du fabriquant. Le champ de vitesse dans l’entrefer du Couette est calculé avec une bonne précision sur un temps inférieur à 1 s et avec une résolution spatiale de 100 µm sur 5mm. La dynamique microscopique d’une solution brownienne est étudiée et l’influence du cisaillement sur la décorrélation est déterminée. Nous montrons les capacités de notre expérience à étudier des matériaux hétérogènes en l’appliquant sur une solution concentrée de micelles géantes. La rhéologie linéaire est étudiée en rhéometrie classique mais aussi en utilisant l’imagerie du speckle. La rhéologie non linéaire de ce matériau est déterminé en rhéometrie (macro) mais aussi en calculant le champ de vitesse et l’intensité des images de speckle (méso) ou on caractérise les bandes de cisaillement qui se forment à partir d’un cisaillement critique. En fin la relaxation spatio-temporelle des bandes de cisaillement (micro) est caractérisée. On observe pour la première fois l’existence de deux temps de relaxation après l’arrêt du cisaillement et que la relaxation des bandes est relativement lente.

This work tries to better characterize the behavior of homogeneous and heterogeneous materials under shear with a multi-scale approach (macro-meso-micro-scopic). To do that, we have developed an innovative setup by coupling a rheometer to a speckle imaging geometry witch is spatially and temporally resolved (RheoSpeckle). We validate our experience using two perfect materials: a solid and a pure viscous fluid. On a solid sample, we calculate the displacement field on the speckle images with a resolution better than 1 µm. we demonstrate than, the microscopic elasticity of this material. On a pure viscous fluid, we measured the nanoparticle’s size with excellent accuracy. When a constant shear rate is applied, the velocity profile is measured with a time less than 1 s with a spatial resolution of 100 µm over 5 mm. The microscopic dynamic of a Brownian solution under shear is probed and the shear induced on the decorrelation of the intensity correlation function is studied. We show the capabilities of our experience using a concentrated solution of wormlike micelles. The linear rheology is studied using rheometric measurements and our speckle imaging system. Nonlinear rheology is studied using rheometric measurements…

Advisors/Committee Members: Roux, Denis (thesis director), Caton, François (thesis director).

Subjects/Keywords: Rhéologie; Matériaux hétérogènes; Speckle; Bandes de cisaillement; Dynamique microscopique; Micelles géantes; Rheology; Heterogeneous materials; Speckle; Shear banding; Microscopic dynamic; Wormlike micelles; 620

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ali, N. (2016). RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2016GREAI013

Chicago Manual of Style (16^th Edition):

Ali, Nabil. “RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales.” 2016. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed January 19, 2021. http://www.theses.fr/2016GREAI013.

MLA Handbook (7^th Edition):

Ali, Nabil. “RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales.” 2016. Web. 19 Jan 2021.

Vancouver:

Ali N. RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2016. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2016GREAI013.

Council of Science Editors:

Ali N. RhéoSpeckle : un nouvel outil d’étude du comportement multi-échelle des matériaux hétérogènes : RheoSpeckle : a new tool for studying the behavior of heterogeneous materials at multi-scales. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2016. Available from: http://www.theses.fr/2016GREAI013

University of Texas – Austin

9. Parthasarathy, Ashwin Bharadwaj. Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging.

Degree: PhD, Biomedical Engineering, 2010, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2010-05-996

► Cerebral blood flow (CBF) measures are central to the investigation of ischemic strokes, spreading depressions, functional and neuronal activation. Laser Speckle Contrast Imaging (LSCI) is… (more)

▼ Cerebral blood flow (CBF) measures are central to the investigation of ischemic strokes, spreading depressions, functional and neuronal activation. Laser Speckle Contrast Imaging (LSCI) is an optical imaging technique that has been used to obtain CBF measures in vivo at high spatial and temporal resolutions, by quantifying the localized spatial blurring of backscattered coherent light induced by blood flow. Despite being widely used for biomedical applications, LSCI's critical limitations such as its tendency to underestimate large flow changes and its inability to accurately estimate CBF through a thinned skull have not been overcome. This dissertation presents a new Multi Exposure Speckle Imaging (MESI) technique that combines a new instrument and mathematical model to overcome these limitations. Additionally, in a pilot clinical study, an adapted neurosurgical microscope was used to obtain intra-operative LSCI images of CBF in humans. The MESI instrument accurately estimates experimental constants by imaging backscattered speckles over a wide range of the camera's exposure durations. The MESI mathematical model helps account for light that has scattered from both static and moving particles. In controlled flow experiments using tissue simulating phantoms, the MESI technique was found to estimate large changes in flow accurately and the estimates of flow changes were found to be unaffected by the presence of static particles in these phantoms. In an in vivo experiment in which the middle cerebral artery in mice was occluded to induce ~100% reduction in CBF, not only was the reduction in CBF accurately estimated by the MESI technique but these estimates of CBF changes were found to be unaffected by the presence of a thinned skull. The validity of statistical models used to derive the MESI mathematical model was confirmed using in vivo dynamic light scattering (DLS) measurements of CBF in mice. The MESI technique's potential to estimate absolute values of CBF in vivo was demonstrated by comparing CBF estimates obtained using the MESI technique to DLS measurements. The MESI technique's ability to measure CBF changes quantitatively through a thinned skull makes it particularly useful in chronic and long term studies leading to the development of better, more accurate stroke models. Advisors/Committee Members: Dunn, Andrew Kenneth, 1970- (advisor), Milner, Thomas (committee member), Tunnell, James (committee member), Emelianov, Stanislav (committee member), Fox, Douglas (committee member).

Subjects/Keywords: Laser Speckle Contrast Imaging; Multi Exposure Speckle Imaging; Optical blood flow measurements; LSCI; MESI; Cerebral blood flow; Ischemic stroke; Speckle spectroscopy; Dynamic Light Scattering; Intra-operative imaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parthasarathy, A. B. (2010). Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2010-05-996

Chicago Manual of Style (16^th Edition):

Parthasarathy, Ashwin Bharadwaj. “Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging.” 2010. Doctoral Dissertation, University of Texas – Austin. Accessed January 19, 2021. http://hdl.handle.net/2152/ETD-UT-2010-05-996.

MLA Handbook (7^th Edition):

Parthasarathy, Ashwin Bharadwaj. “Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging.” 2010. Web. 19 Jan 2021.

Vancouver:

Parthasarathy AB. Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152/ETD-UT-2010-05-996.

Council of Science Editors:

Parthasarathy AB. Quantitative cerebral blood flow measurement with Multi Exposure Speckle Imaging. [Doctoral Dissertation]. University of Texas – Austin; 2010. Available from: http://hdl.handle.net/2152/ETD-UT-2010-05-996

University of Texas – Austin

10. Davis, Mitchell Alan. Three dimensional simulation of functional neuro-vascular imaging.

Degree: PhD, Electrical and Computer Engineering, 2014, University of Texas – Austin

URL: http://hdl.handle.net/2152/38225

► Functional optical imaging has become a powerful tool for measuring physiological parameters in the brain without disrupting normal physiology. Fluorescence lifetime imaging (FLIM) has been… (more)

▼ Functional optical imaging has become a powerful tool for measuring physiological parameters in the brain without disrupting normal physiology. Fluorescence lifetime imaging (FLIM) has been shown to allow near real time mapping of oxygen tension in plasma (pO2), and Laser Speckle Contrast Imaging (LSCI) has been demonstrated to provide qualitative assessments of blood flow in the cortex. However, as both of these methods provide physiological parameters based on the spatial sampling of photons arriving at a detector, it is crucially important to understand either where the photons originated, in the case of FLIM, or which moving particles the photons have sampled, in the case of LSCI. Traditionally, these questions have been difficult to solve because of the heterogeneity of the distribution of particles which contribute to the measured signal. In both FLIM and LSCI, for example, only the light which samples the intravascular space will contribute to the signal. While analytical methods have proven to be successful at predicting the imaging depth of homogeneous materials, they are not able to predict imaging depth when measuring a fluorophore or a moving particle that is only present inside blood vessels. Unlike analytical methods, numerical methods can be used to approximate light propagation in an arbitrary geometry. While both deterministic and stochastic models of light propagation can, and have been, successfully employed to determine light fluence in an arbitrary geometry, deterministic methods are not well suited to the task of simulating light propagation in large volumes of turbid media. For this reason, three dimensional Monte Carlo simulations of light propagation combined with high resolution vascular anatomy were used to directly simulate FLIM and LSCI in the brain. Using these simulations, the imaging depth, degree of multiple scattering, and sensitivity of LSCI and FLIM to physiological changes were determined. Advisors/Committee Members: Dunn, Andrew Kenneth, 1970- (advisor), Pearce, John (committee member), Thomas, Robert (committee member), Tunnell, James (committee member), Ling, Hao (committee member).

Subjects/Keywords: Laser-tissue interactions; Vascular fluorescence imaging; Speckle contrast imaging; Dynamic light scattering; Blood flow imaging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Davis, M. A. (2014). Three dimensional simulation of functional neuro-vascular imaging. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/38225

Chicago Manual of Style (16^th Edition):

Davis, Mitchell Alan. “Three dimensional simulation of functional neuro-vascular imaging.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed January 19, 2021. http://hdl.handle.net/2152/38225.

MLA Handbook (7^th Edition):

Davis, Mitchell Alan. “Three dimensional simulation of functional neuro-vascular imaging.” 2014. Web. 19 Jan 2021.

Vancouver:

Davis MA. Three dimensional simulation of functional neuro-vascular imaging. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152/38225.

Council of Science Editors:

Davis MA. Three dimensional simulation of functional neuro-vascular imaging. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/38225

University of Toronto

11. Weatherbee, Andrew Stephen. A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function.

Degree: 2017, University of Toronto

URL: http://hdl.handle.net/1807/79326

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This thesis explores theory and data processing techniques that have the potential to extract much more information about a sample than can be gathered from… (more)

Subjects/Keywords: Brownian motion; Dynamic light scattering; Optical coherence tomography; Scattering; Speckle; Viscosity; 0786

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weatherbee, A. S. (2017). A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/79326

Chicago Manual of Style (16^th Edition):

Weatherbee, Andrew Stephen. “A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function.” 2017. Masters Thesis, University of Toronto. Accessed January 19, 2021. http://hdl.handle.net/1807/79326.

MLA Handbook (7^th Edition):

Weatherbee, Andrew Stephen. “A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function.” 2017. Web. 19 Jan 2021.

Vancouver:

Weatherbee AS. A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1807/79326.

Council of Science Editors:

Weatherbee AS. A study of the statistical properties of scattered radiation under optical coherence tomography conditions: scattering medium characterization via the power spectrum and the probability density function. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/79326

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