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You searched for subject:(dipeptidyl peptidase III). Showing records 1 – 2 of 2 total matches.

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Linköping University

1. Jansson, Lennie. Purification and refolding of a novel dipeptidyl peptidase III.

Degree: Chemistry, 2019, Linköping University

There is a continuous search for novel enzymes to complement the abilities of today’s commercially available enzyme and find tailor-fit alternatives to suit the diverse array of bio-based industries. One application could be to increase biogas yield by finding substrate degrading proteases that can be added to the anaerobic digestion process and survive degradation themselves. A novel enzyme identified as a hypothetical dipeptidyl peptidase III, a zinc dependent metallo-protease, was found by a metaproteogenomics approach to be produced by the microorganisms of a thermophilic biogas process. The aim of this study was to express and refold a recombinant variant of the novel DPP III to its active form after production in inclusion bodies in Escherichia Coli. Assaying of refolding conditions was performed by stepwise dialysis and drip dilution. Nine attempts were performed based on findings in literature, although no other variant of DPP III has earlier been successfully refolded from inclusion bodies. The study resulted in a limited set of conditions of temperature, volumes, metal ions, salts and other additives being tested in the refolding buffers. Enzyme refolding and activation was monitored by the hydrolysis of the DPP III fluorescent substrate Arg-Arg β-naphthylamide trihydrochloride, alongside with measurements of protein concentration and SDS-PAGE. The novel DPP III was successfully purified but no definite strategy of producing correctly folded protein was found.

Subjects/Keywords: dipeptidyl peptidase III; DPP III; purification; refolding; protein; Arg-Arg-2NA; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jansson, L. (2019). Purification and refolding of a novel dipeptidyl peptidase III. (Thesis). Linköping University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Jansson, Lennie. “Purification and refolding of a novel dipeptidyl peptidase III.” 2019. Thesis, Linköping University. Accessed June 26, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Jansson, Lennie. “Purification and refolding of a novel dipeptidyl peptidase III.” 2019. Web. 26 Jun 2019.

Vancouver:

Jansson L. Purification and refolding of a novel dipeptidyl peptidase III. [Internet] [Thesis]. Linköping University; 2019. [cited 2019 Jun 26]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jansson L. Purification and refolding of a novel dipeptidyl peptidase III. [Thesis]. Linköping University; 2019. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Queensland University of Technology

2. Jamriska, Lubomira. Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum.

Degree: 2000, Queensland University of Technology

Subjects/Keywords: Schistosoma japonicum; Peptidase; dipeptidyl peptidase I; DPP I; DPP III; cathespin C; protease; schistosome; Schistosoma japonicum; proregion peptide; baculovirus; insect cells; Trichoplusia ni; thesis; doctoral

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jamriska, L. (2000). Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/37079/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Jamriska, Lubomira. “Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum.” 2000. Thesis, Queensland University of Technology. Accessed June 26, 2019. http://eprints.qut.edu.au/37079/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Jamriska, Lubomira. “Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum.” 2000. Web. 26 Jun 2019.

Vancouver:

Jamriska L. Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum. [Internet] [Thesis]. Queensland University of Technology; 2000. [cited 2019 Jun 26]. Available from: http://eprints.qut.edu.au/37079/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jamriska L. Characterisation of dipeptidyl peptidase I of the Asian blood fluke, Schistosoma japonicum. [Thesis]. Queensland University of Technology; 2000. Available from: http://eprints.qut.edu.au/37079/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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