subject:(dinitrophenol)

1. Carlos Roberto Porto Dechandt. Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR).

Degree: 2018, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/17/17131/tde-13092018-104230/

►

Introdução: Wistar Audiogenic Rats (WAR) é modelo experimental desenvolvido na Faculdade de Medicina de Ribeirão Preto, para estudo da epilepsia, entretanto, a seleção genética em… (more)

▼

Introdução: Wistar Audiogenic Rats (WAR) é modelo experimental desenvolvido na Faculdade de Medicina de Ribeirão Preto, para estudo da epilepsia, entretanto, a seleção genética em resposta aos comportamentos de crises audiogênicas também trouxe à tona alterações no metabolismo energético nesses animais. Dois estudos são relevantes deste ponto de vista, no primeiro Botion e Doretto observaram que WAR após serem estimulados apresentam: (a) valores de glicemia maior em relação ao Wistar; (b) aumento no lactato circulante - o que pode indicar deficiência na fosforilação oxidativa (OXPHOS); (c) aumento da atividade adrenérgica, induzindo dessensibilização da via lipolítica ?-adrenérgica no tecido adiposo epididimal. Pereira e colaboradores investigando o metabolismo de carboidratos relataram: (a) aumento na via glicólica; (b) translocação de GLUT4 aumentada no músculo gastrocnêmico e (c) redução nos níveis de glicogênio muscular. Objetivo: Diante desses achados o presente estudo tem como objetivo elucidar o metabolismo energético mitocondrial e sua relação com as crises epiléticas induzidas por estímulos sonoros. Resultados: Através de analises comportamentais e calorimetria indireta, relatamos que WAR possui perfil ansiogênico e tem preferência em oxidar aminoácidos; utilizando biopsias de tecido hepático, musculo esquelético e cardíaco, observamos maior densidade mitocondrial, acompanhada de maior geração de H2O2 e como consequência maior estresse oxidativo; na busca para elucidar com maior destreza, realizamos isolamento da fração mitocondrial do tecido hepático, e concluímos que não há maior geração de H2O2 por mitocôndria, porém há um enriquecimento proteico (algumas proteínas funcionais - como as envolvidas na OXPHOS- e outras não-funcionais - como as UCPs), além de relatarmos maiores níveis de proteínas envolvidas na dinâmica mitocondrial, desse modo podemos concluir que WAR possuem maior densidade mitocondrial e mitocôndrias com maior eficiência; o mesmo perfil mitocondrial foi observado no tecido cerebral. Após tratamento com DNP e NAC, observamos redução do estresse oxidativo no tecido cerebral, porém apenas NAC reduziu a severidade da crise, assim concluímos que o suave desacoplamento induzido por DNP não é capaz de reduzir de forma significativa a severidade da crise, porém a inibição da glicólise pelo NAC, alterou a bioenergética cerebral é capaz a reduzir a severidade da crise, deixando evidenciado que o metabolismo energético tem papel essencial/relevante na crise epilética induzida por estímulos sonoros em WAR, enquanto o estresse oxidativo tem papel secundário.

Introduction: Wistar Audiogenic Rats (WAR) is an experimental model developed in the Ribeirão Preto Medical School, for the study of epilepsy, however, the genetic selection in response to the behaviors of audiogenic crisis also brought up changes in energy metabolism in these animals. Two studies are relevant from this point of view, in the first Botion and Doretto observed that WAR after being stimulated present: (a) higher blood…

Advisors/Committee Members: Luciane Carla Alberici, Amanda Martins Baviera, Tiago Rezende Figueira, Adriano Silva Sebollela, Sergio Akira Uyemura.

Subjects/Keywords: 2,4-Dinitrofenol - DNP; N-acetilcisteína - NAC; Mitocôndria; Wistar audiogenic rats - WAR; Espécies reativas de oxigênio - EROs; Epilepsia audiogênica; 2.4-Dinitrophenol - DNP; Audiogenic epilepsy; Mitochondria; N-acetylcysteine - NAC; Reactive oxygen species - EROs; Wistar audiogenic Rats-WAR

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dechandt, C. R. P. (2018). Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR). (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/17/17131/tde-13092018-104230/

Chicago Manual of Style (16^th Edition):

Dechandt, Carlos Roberto Porto. “Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR).” 2018. Doctoral Dissertation, University of São Paulo. Accessed April 13, 2021. http://www.teses.usp.br/teses/disponiveis/17/17131/tde-13092018-104230/.

MLA Handbook (7^th Edition):

Dechandt, Carlos Roberto Porto. “Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR).” 2018. Web. 13 Apr 2021.

Vancouver:

Dechandt CRP. Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR). [Internet] [Doctoral dissertation]. University of São Paulo; 2018. [cited 2021 Apr 13]. Available from: http://www.teses.usp.br/teses/disponiveis/17/17131/tde-13092018-104230/.

Council of Science Editors:

Dechandt CRP. Estudo do metabolismo energético mitocondrial e sua relação com as crises epiléticas em Wistar audiogenic rats (WAR). [Doctoral Dissertation]. University of São Paulo; 2018. Available from: http://www.teses.usp.br/teses/disponiveis/17/17131/tde-13092018-104230/

University of Central Florida

2. Cooley, Gianna. Removal Of 2, 4-dinitrophenol By Ferrate.

Degree: 2008, University of Central Florida

URL: https://stars.library.ucf.edu/etd/3619

► Ferrate (molecular formula, FeVIO42-) has been studied increasingly since the 1970s as a disinfectant and coagulant for domestic wastewater and also as an oxidant for… (more)

▼ Ferrate (molecular formula, FeVIO42-) has been studied increasingly since the 1970s as a disinfectant and coagulant for domestic wastewater and also as an oxidant for industrial wastewaters (Murmann and Roginson, 1974, Gilbert et al., 1978, Kazama, 1994, Jiang et al., 2002, and Sharmaet al., 2005). This research was performed to explore whether ferrate could possibly be used as chemical treatment for industrial wastewaters from plastic, chemical, dye, soap, and wood stain producing plants that contain 2, 4-Dinitrophenol (DNP). DNP is listed on the United States Environmental Protection Agency (EPA) Drinking Water Contaminant Candidate List (CCL). This list includes compounds which are not currently regulated at the national level, but there is a growing concern for the harm they may cause to the environment. Therefore, the EPA prioritizes these compounds and conducts extensive research to determine if these compounds should be regulated (USEPA, 2005). The effects of Ferrate on DNP were evaluated during these experiments. The effect of various dosages of Ferrate and different pH values was monitored over 17 minutes using UV 254 to determine the extent of oxidation of 300 mg L-1 DNP. Removal of DNP at all pHs and dosages was noted, however, a pH of 4 and a molar ratio of 14: 1 (Ferrate to DNP) removed the highest percentage of DNP at 87.3. The by-products of the 3.5 and 14: 1 molar ratio of Ferrate to DNP reactions at a pH of 4 and their toxicity were determined by measuring biochemical oxygen demand 5 day (BOD5), dissolved organic carbon (DOC), chlorine residual and chemical oxygen demand (COD), and gas chromatography/mass spectrometry (GC/MS) analysis. The BOD5 indicated toxicity, either from the residual chlorine or the organisms used for seeding not being acclimated to DNP and by-products. DOC of the 3.5 : 1 molar ratio was higher than calculated values indicating more ring breakage than was originally measured by UV 254. DOC of the 14: 1 molar ratio experiment was lower than calculated values, which indicated human error in measuring the DNP concentration. The chlorine residual was high for both experiments, 112 and 594 mg L-1, for the 3.5 and 14: 1 molar ratios, respectively. COD was unable to be measured due to chloride interference. The GC/MS data showed several chlorine-substituted benzene rings as well as carbon tetrachloride for the 3.5:1 molar ratio DNP experiments. The 14:1 GC/MS data indicated much more ring breakage with carbon tetrachloride, a substituted butane chain, many unknown straight chain chlorinated compounds and dichloro-pentane isomers as by-products. Advisors/Committee Members: Reinhart, Debra.

Subjects/Keywords: Ferrate; 2; 4-Dinitrophenol; Engineering; Environmental Engineering

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cooley, G. (2008). Removal Of 2, 4-dinitrophenol By Ferrate. (Masters Thesis). University of Central Florida. Retrieved from https://stars.library.ucf.edu/etd/3619

Chicago Manual of Style (16^th Edition):

Cooley, Gianna. “Removal Of 2, 4-dinitrophenol By Ferrate.” 2008. Masters Thesis, University of Central Florida. Accessed April 13, 2021. https://stars.library.ucf.edu/etd/3619.

MLA Handbook (7^th Edition):

Cooley, Gianna. “Removal Of 2, 4-dinitrophenol By Ferrate.” 2008. Web. 13 Apr 2021.

Vancouver:

Cooley G. Removal Of 2, 4-dinitrophenol By Ferrate. [Internet] [Masters thesis]. University of Central Florida; 2008. [cited 2021 Apr 13]. Available from: https://stars.library.ucf.edu/etd/3619.

Council of Science Editors:

Cooley G. Removal Of 2, 4-dinitrophenol By Ferrate. [Masters Thesis]. University of Central Florida; 2008. Available from: https://stars.library.ucf.edu/etd/3619

University of Arizona

3. Cozine, William Samuel, 1938-. Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds .

Degree: 1965, University of Arizona

URL: http://hdl.handle.net/10150/318403

Subjects/Keywords: Immune response.; Allergy.; Dinitrophenol.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cozine, William Samuel, 1. (1965). Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/318403

Chicago Manual of Style (16^th Edition):

Cozine, William Samuel, 1938-. “Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds .” 1965. Masters Thesis, University of Arizona. Accessed April 13, 2021. http://hdl.handle.net/10150/318403.

MLA Handbook (7^th Edition):

Cozine, William Samuel, 1938-. “Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds .” 1965. Web. 13 Apr 2021.

Vancouver:

Cozine, William Samuel 1. Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds . [Internet] [Masters thesis]. University of Arizona; 1965. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10150/318403.

Council of Science Editors:

Cozine, William Samuel 1. Hypersensitive and immune response in rabbits to 2,4-dinitrophenyl compounds . [Masters Thesis]. University of Arizona; 1965. Available from: http://hdl.handle.net/10150/318403

University of Saskatchewan

4. Marit, Jordan Scott. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).

Degree: 2010, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-11082010-181618

► Examination of the swimming capabilities of fish is increasingly being considered as an effective method for determining sublethal toxicity. Acute toxicant exposure is known to… (more)

▼ Examination of the swimming capabilities of fish is increasingly being considered as an effective method for determining sublethal toxicity. Acute toxicant exposure is known to cause decreases in swim performance in fish but less is known about how developmental exposure can cause persistent effects that hinder swimming. In addition, little is known about how triglyceride levels fluctuate during fish swimming upon both acute and developmental exposure to toxicant. In this thesis, two studies, one acute and one developmental, were carried out using two different toxicants in order to address these issues. In order to examine acute effects, adult zebrafish (Danio rerio) were exposed to ethanol vehicle or increasing concentrations of 2,4-dinitrophenol (DNP), a mitochondrial electron transport chain uncoupler, for a 24 h period. Following exposure, fish were placed in a swim tunnel for critical swimming speed (Ucrit) determination and swim motion analysis. Whole body triglyceride levels were then determined. Ucrit was decreased in a concentration dependent manner in both the 6 mg/L and 12 mg/L DNP exposure groups, with 6 mg/L DNP being considered sublethal and 12 mg/L approaching the LC50. A decrease in tail beat frequency was observed and is likely the main cause for the decrease in Ucrit in the DNP exposure groups. Triglyceride levels were elevated in a concentration dependent manner in the DNP exposure groups. This increase in triglyceride stores may be due to a behavioral adaption limiting swimming capabilities or due to a direct toxic action of DNP on lipid catabolism. The second study examined whether developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure would cause persistent toxic effects. Zebrafish embryos were exposed to dimethyl sulfoxide control or increasing concentrations of TCDD between 2-4 days post fertilization (dpf). At 5 dpf, cytochrome P450 1A (CYP1A) activity was determined. Fish were raised to 90 dpf with mortalities and deformities being recorded at 5 dpf, 10 dpf, and 90 dpf. At 90 dpf, fish were placed in swim tunnel and Ucrit , swimming motion, and aerobic scope (oxygen consumption rate during exercise minus oxygen consumption rate during rest) were determined. Following swimming, some fish were used for whole body triglyceride analysis while others were used for histological examination. Ucrit was shown to be decreased in the two highest sublethal TCDD exposure groups (0.1 and 1 ng/L) but not in the lowest TCDD exposure group (0.01 ng/L). The exact cause of the decrease in Ucrit is not known, but may be linked to the observed decrease in dorsal aorta diameter, an inability to mobilize triglyceride stores, behavioral adaptations limiting swimming, decreased body length, or a combination of these factors. This TCDD related defect in swimming ability is not due to any increases in gross deformity or mortality rates, nor does it appear that CYP1A induction is required to mediate the toxic effects. Thus, it appears that examination of swim performance may serve as an effective measure… Advisors/Committee Members: Weber, Lynn, Jones, Paul, Muir, Gillian, Niyogi, Som.

Subjects/Keywords: zebrafish; dioxin; triglycerides; dinitrophenol; sublethal toxicity; critical swimming speed

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Marit, J. S. (2010). Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-11082010-181618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Marit, Jordan Scott. “Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).” 2010. Thesis, University of Saskatchewan. Accessed April 13, 2021. http://hdl.handle.net/10388/etd-11082010-181618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Marit, Jordan Scott. “Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio).” 2010. Web. 13 Apr 2021.

Vancouver:

Marit JS. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). [Internet] [Thesis]. University of Saskatchewan; 2010. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10388/etd-11082010-181618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marit JS. Swim performance as an effective, environmentally relevant measure of sublethal toxicity in zebrafish (Danio rerio). [Thesis]. University of Saskatchewan; 2010. Available from: http://hdl.handle.net/10388/etd-11082010-181618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

5. Mossie, Godwin Mxolisi Kevin. Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain.

Degree: MS, Faculty of Science, Biochemistry, Microbiology and Biotechnology, 1980, Rhodes University

URL: http://hdl.handle.net/10962/d1013543

► Bacteroides fragilis strain Bf-1 produces an extracellular bacteriocin at the beginning of the stationary growth phase. Production is not inducible by either ultraviolet light or… (more)

Subjects/Keywords: Bacteroides; Anaerobic bacteria; Trypsin; Dinitrophenol; Proteins – Synthesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mossie, G. M. K. (1980). Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain. (Masters Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1013543

Chicago Manual of Style (16^th Edition):

Mossie, Godwin Mxolisi Kevin. “Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain.” 1980. Masters Thesis, Rhodes University. Accessed April 13, 2021. http://hdl.handle.net/10962/d1013543.

MLA Handbook (7^th Edition):

Mossie, Godwin Mxolisi Kevin. “Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain.” 1980. Web. 13 Apr 2021.

Vancouver:

Mossie GMK. Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain. [Internet] [Masters thesis]. Rhodes University; 1980. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10962/d1013543.

Council of Science Editors:

Mossie GMK. Characterization and mode of action of a bacteriocin produced by a Bacteroides Fragilis strain. [Masters Thesis]. Rhodes University; 1980. Available from: http://hdl.handle.net/10962/d1013543

6. Schlagowski, Anna - Isabel. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.

Degree: Docteur es, Physiologie et biologie des organismes, populations, interactions, 2014, Université de Strasbourg

URL: http://www.theses.fr/2014STRAJ105

►

Les mécanismes impliqués dans les adaptations du phénotype métabolique musculaire au cours de l’exercice physique restent imparfaitement connus. Nous nous sommes intéressés au concept d’hormèse… (more)

▼

Les mécanismes impliqués dans les adaptations du phénotype métabolique musculaire au cours de l’exercice physique restent imparfaitement connus. Nous nous sommes intéressés au concept d’hormèse mitochondriale qui se définit comme un stress métabolique activant les voies de signalisation menant à une activation mitochondriale.En première partie, nous avons validé l’utilisation d’un nouveau système de mesure des échanges gazeux chez le rat au cours de différents exercices sur tapis roulant, et démontré que pour une vitesse de course sous maximale, un exercice en descente sollicite le système cardiovasculaire de façon modérée sans altérer la fonction mitochondriale musculaire, ni augmenter la production de radicaux libres oxygénés.En deuxième partie, nous avons montré qu’un découplage mitochondrial provoqué par un traitement des rats au 2,3-dinitrophénol (DNP) pendant 3 semaines engendre des adaptations métaboliques menant à l’augmentation de la masse mitochondriale du muscle squelettique. Ces animaux ont une capacité à l’exercice diminuée, malgré une augmentation de leur VO2max.Pour finir, nous avons montré qu’un préconditionnement par l’exercice protège la mitochondrie musculaire squelettique des effets délétères de l’ischémie-reperfusion. L’exercice semble activer le métabolisme via un phénomène d’hormèse mitochondriale permettant la protection musculaire. En conclusion, cette thèse nous montre d’une part l’importance de la mitochondrie (aspect quantitatif mais surtout qualitatif) en terme de limitation à l’exercice, et d’autre part nous suggère que l'optimisation du fonctionnement mitochondrial pourrait être une bonne garantie pour pouvoir lutter efficacement contre les stress, notamment oxydatifs, auxquels l'organisme est soumis en (quasi)permanence.

The mechanisms regulating the metabolic phenotype adaptations in skeletal muscle during physical exercise is still unknown.We studied the mitochondrial hormesis phenomenon that could be defined as a metabolic stress activating the signaling pathways leading to a mitochondrial stimulation (mitochondrial biogenesis).In the first part, we validated the utilization of a new system determining the gas exchange in rat during a treadmill exhaustive exercise. We showed that a submaximal downhill exercise activate moderately the cardiovascular system, without mitochondrial functional impairments and without any augmentation of the systemic ROS production. In the second part, we showed that a mitochondrial uncoupling following a dinitrophenol treatment during 3 weeks in rats induced some metabolic adaptations leading to a higher mitochondrial mass in skeletal muscle. The exercise capacity of these animals is reduced whereas the maximal oxygen consumption is higher.In the last part, we showed that a preconditioning protocol with an acute exercise protected the skeletal muscle mitochondria from the deleterious effects of ischemia-reperfusion. This exercise seems to activate the muscular metabolism via a phenomenon of mitochondrial hormesis activation, allowing an efficient…

Advisors/Committee Members: Piquard, François (thesis director).

Subjects/Keywords: Mitochondrie; Stress oxydant; Exercice; Muscle; Consommation d'oxygène; Dinitrophénol; Découplage; Skeletal muscle; Physical exercise; Mitochondria; Oxygen consumption; Dinitrophenol treatment; Oxidative stress; 573.7; 572.8

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schlagowski, A. -. I. (2014). Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2014STRAJ105

Chicago Manual of Style (16^th Edition):

Schlagowski, Anna - Isabel. “Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.” 2014. Doctoral Dissertation, Université de Strasbourg. Accessed April 13, 2021. http://www.theses.fr/2014STRAJ105.

MLA Handbook (7^th Edition):

Schlagowski, Anna - Isabel. “Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis.” 2014. Web. 13 Apr 2021.

Vancouver:

Schlagowski A-I. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2014. [cited 2021 Apr 13]. Available from: http://www.theses.fr/2014STRAJ105.

Council of Science Editors:

Schlagowski A-I. Etude des adaptations mitochondriales dans le muscle squelettique : importance de l'hormèse mitochondriale : Study of mitochondrial adaptations in skeletal muscle : role of mitochondrial hormesis. [Doctoral Dissertation]. Université de Strasbourg; 2014. Available from: http://www.theses.fr/2014STRAJ105

Louisiana State University

7. Snow, Lynne A. Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog.

Degree: MS, Veterinary Medicine, 2010, Louisiana State University

URL: etd-04272010-221042 ; https://digitalcommons.lsu.edu/gradschool_theses/672

► Objectives 1) To measure conductance and permeability of canine colonic mucosa exposed to increasing concentrations of carprofen. 2) To compare conductance and permeability of… (more)

▼ Objectives 1) To measure conductance and permeability of canine colonic mucosa exposed to increasing concentrations of carprofen. 2) To compare conductance and permeability of canine colonic mucosa exposed to carprofen or 2,4-dinitrophenol (DNP) and tempol blockade. Design In vitro randomized block design Animal 20 mixed breed dogs Methods Conductance, mannitol flux, and histology were evaluated in colonic mucosa mounted in Ussing chambers. Mucosa was first exposed to increasing concentrations of carprofen. Mucosa was then exposed to either carprofen (200 μg/ml) or DNP (0.25mM) +/- tempol (1mM) pretreatment. Conductance over time, mannitol fluxes, and frequency of histologic categories were analyzed for treatment effects. Histopathology and electron microscopy were evaluated post experiment. Results Mean +/- SEM conductance*time for 400 μg/ml carprofen treated colon was significantly greater than control. Mean +/- SEM conductance*time for carprofen treated colon at 200, 100 and 40 μg/ml were not significantly different from control. Mean +/- SEM conductance*time for 400 μg/ml and 200 μg/ml carprofen treated colon were not significantly different. Period 3 mannitol flux was greater than period 1 for 400 μg/ml and 200 μg/ml carprofen treated colon but not significantly different for 100 μg/ml, 40 μg/ml, and control. Period 3 flux for 400 μg/ml and 200 μg/ml carprofen treated colon were not different but were greater than control. Mean +/- SEM conductance*time for carprofen or DNP treated colon were not significantly different from control regardless of blockade. Period 3 flux for carprofen and DNP treated colon were not different but were greater than control. Period 3 flux for carprofen treated colon with tempol pretreatment was not significantly different than control. Period 3 flux for DNP treated colon with tempol pretreatment was not different than without tempol but was greater than control. Cell sloughing and erosions were observed with high carprofen concentrations. Mitochondrial damage was seen with carprofen treatment compared to DNP treatment or control. Tempol pretreatment effect on mitochondrial morphology was inconsistent. Conclusion Carprofen exhibits concentration dependent toxicity to canine colonic mucosa. Carprofen and DNP induce similar mucosal damage evident by changes in electrical conductance, mannitol flux, and histopathology. Carprofen damages enterocyte mitochondria.

Subjects/Keywords: dinitrophenol; tempol; carprofen; oxidative stress; mitochondria; colon; DNP

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Snow, L. A. (2010). Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog. (Masters Thesis). Louisiana State University. Retrieved from etd-04272010-221042 ; https://digitalcommons.lsu.edu/gradschool_theses/672

Chicago Manual of Style (16^th Edition):

Snow, Lynne A. “Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog.” 2010. Masters Thesis, Louisiana State University. Accessed April 13, 2021. etd-04272010-221042 ; https://digitalcommons.lsu.edu/gradschool_theses/672.

MLA Handbook (7^th Edition):

Snow, Lynne A. “Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog.” 2010. Web. 13 Apr 2021.

Vancouver:

Snow LA. Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog. [Internet] [Masters thesis]. Louisiana State University; 2010. [cited 2021 Apr 13]. Available from: etd-04272010-221042 ; https://digitalcommons.lsu.edu/gradschool_theses/672.

Council of Science Editors:

Snow LA. Carprofen-induced oxidative stress in mitochondria of the colonic mucosa of the dog. [Masters Thesis]. Louisiana State University; 2010. Available from: etd-04272010-221042 ; https://digitalcommons.lsu.edu/gradschool_theses/672

University of New Mexico

8. Vaughan, Roger. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.

Degree: Individual, Family, and Community Education, 2011, University of New Mexico

URL: http://hdl.handle.net/1928/12800

► Obesity is one of the most prevalent maladies in the United States and is a major cause of preventable death. Weight loss supplements frequently claim… (more)

▼ Obesity is one of the most prevalent maladies in the United States and is a major cause of preventable death. Weight loss supplements frequently claim uncoupling as a mechanism of action. Uncoupling agents could be used for weight loss because they disrupt mitochondrial metabolism thereby reducing adenosine triphosphate (ATP) yield. Consequently, metabolic efficiency diminishes increasing basal metabolic rate. 2,4-Dinitrophenol (DNP) successfully uncouples but was banned in 1938 because of a narrow window between efficacy and toxicity. PURPOSE: To measure the ability of a blend that reportedly contains 17-dihydroxy-delta-5-etiocholane-7-one and p-methylcarbonylethylphenol and other substances (A7E), a purported uncoupling agent, to interfere with oxidative phosphorylation in Saccharomyces cerevisiae as evidenced by lower ATP production. METHODS: Timed culture studies of S. cerevisiae were performed using two separate agents, A7E (a purported uncoupling agent) and DNP (a known uncoupling agent) at three doses (DNP: Low, Moderate, High; A7E: Low, Moderate, High ), and an ethanol-treated control to detect interference with mitochondrial coupling. Microbial staining was used to ascertain cell viability and any changes in cell densities. ATP production was estimated by measuring luminosity generated in culture supernatants using the ATP Bioluminescence Kit (Sigma St. Louis, MO.). RESULTS: Luminosity measurements estimating ATP production revealed statistically lower ATP in agent-treated supernatants than in control supernatants (p < 0.001) except for low dose A7E versus control (p > .05), suggesting that both A7E and DNP acted by a mechanism of uncoupling. Luminosity values were measured in relative luminosity units (RLU). Treatments with A7E at Low, Moderate, and High doses generated group mean luminosity values of 24,596, 16,038, and 6,969, respectively. Treatments with DNP at Low, Moderate, and High doses generated group mean luminosity values of 17,191, 11,901, 767 RLU respectively. The control group mean was 31,645 RLU. Culture studies had no statistical difference (p > 0.0167 adjusted) in total and viable cell densities between the control and A7E and DNP treatments. CONCLUSION: A7E is effective at reducing ATP levels in this assay, as is known uncoupling agent DNP, supporting the hypothesis that A7E may also uncouple oxidative phosphorylation. Because A7E requires a higher dosage than DNP to equivalently disrupt mitochondrial metabolism, it may have a wider range of therapeutic doses. This suggests that A7E should be studied further for safety and efficacy with respect to metabolic efficiency and weight loss. Advisors/Committee Members: Conn, Carole, Lockner, Donna, Conn, Carole, Dichosa, Armand.

Subjects/Keywords: Phosphorylation.; Adenosine triphosphate.; Dinitrophenol.; Mitochondria – Physiology.; Saccharomyces cerevisiae – Metabolism.; Obesity – Treatment.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Vaughan, R. (2011). Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. (Masters Thesis). University of New Mexico. Retrieved from http://hdl.handle.net/1928/12800

Chicago Manual of Style (16^th Edition):

Vaughan, Roger. “Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.” 2011. Masters Thesis, University of New Mexico. Accessed April 13, 2021. http://hdl.handle.net/1928/12800.

MLA Handbook (7^th Edition):

Vaughan, Roger. “Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity.” 2011. Web. 13 Apr 2021.

Vancouver:

Vaughan R. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. [Internet] [Masters thesis]. University of New Mexico; 2011. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/1928/12800.

Council of Science Editors:

Vaughan R. Evaluating mitochondrial uncoupling potentials of A7E and DNP in Saccharomyces cerevisiae : implications for human obesity. [Masters Thesis]. University of New Mexico; 2011. Available from: http://hdl.handle.net/1928/12800

Iowa State University

9. Viado, Getulio Bersamira. Some internal effects of dinitrophenols on insects.

Degree: 1941, Iowa State University

URL: https://lib.dr.iastate.edu/rtd/13541

Subjects/Keywords: Dinitrophenol; Insecticides; Entomology; Entomology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Viado, G. B. (1941). Some internal effects of dinitrophenols on insects. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/rtd/13541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Viado, Getulio Bersamira. “Some internal effects of dinitrophenols on insects.” 1941. Thesis, Iowa State University. Accessed April 13, 2021. https://lib.dr.iastate.edu/rtd/13541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Viado, Getulio Bersamira. “Some internal effects of dinitrophenols on insects.” 1941. Web. 13 Apr 2021.

Vancouver:

Viado GB. Some internal effects of dinitrophenols on insects. [Internet] [Thesis]. Iowa State University; 1941. [cited 2021 Apr 13]. Available from: https://lib.dr.iastate.edu/rtd/13541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Viado GB. Some internal effects of dinitrophenols on insects. [Thesis]. Iowa State University; 1941. Available from: https://lib.dr.iastate.edu/rtd/13541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

10. Yang, Fei. Production of pyruvate by Escherichia coli using metabolic engineering.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22034

► Pyruvate is a three-carbon ketoacid produced at the end of glycolysis. This research involves the metabolic engineering of the bacterium Escherichia coli in order to… (more)

Subjects/Keywords: Pyruvate; Escherichia coli; Pyruvate dehydrogenase complex, Phosphoenolpyruvate carboxylase; Pyruvate oxidase; NADH oxidase; 2,4-dinitrophenol, Plackett-Burman design

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yang, F. (2014). Production of pyruvate by Escherichia coli using metabolic engineering. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Thesis, University of Georgia. Accessed April 13, 2021. http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yang, Fei. “Production of pyruvate by Escherichia coli using metabolic engineering.” 2014. Web. 13 Apr 2021.

Vancouver:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 13]. Available from: http://hdl.handle.net/10724/22034.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yang F. Production of pyruvate by Escherichia coli using metabolic engineering. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22034

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations