subject:(developmental biology)

University of Helsinki

1. Matsuda, Shinya. The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing.

Degree: Department of Biosciences, Division of Genetics; Institute of Biotechnology, 2013, University of Helsinki

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Evolutionarily conserved signaling pathways mediate cell-cell communications during development. While the extracellular signal is precisely regulated to achieve dynamic morphogenetic events at the species level,… (more)

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Evolutionarily conserved signaling pathways mediate cell-cell communications during development. While the extracellular signal is precisely regulated to achieve dynamic morphogenetic events at the species level, it must be also flexible to generate the diversified morphologies through evolution. However, little is known about the mechanisms behind the precision and flexibility. The insect wing vein pattern can provide an excellent model to address this fundamental question, since species-specific wing vein patterns have been diversified through evolution. In this thesis, I study how evolutionarily conserved bone morphogenetic protein (BMP)-type ligand specifies diversified insect wing vein patterns using Dipteran Drosophila melanogaster and Hymenopteran sawfly Athalia rosae as models. In Drosophila, BMP-type ligand Decapentaplegic (Dpp) is expressed in the longitudinal veins (LVs) to maintain LVs and induce crossveins (CVs) fates during pupal stages. However, the distribution of Dpp remained largely unknown. Using GFP-tagged Dpp, I demonstrated that Dpp is directionally transported from LVs into the posterior crossvein (PCV) region by two extracellular BMP-binding proteins, Short gastrulation (Sog) and Crossveinless (Cv). In contrast, most of Dpp did not diffuse from LVs by Type I BMP receptor and a positive feedback mechanism. Thus the active transport and retention mechanisms allow diffusible Dpp to draw the complex wing vein patterns in Drosophila. To investigate how BMP signal instructs wing vein morphogenesis that involves apposition and cell shape changes between two wing epithelial layers, I then focused on the function of RhoGAP Crossveinless-C (Cv-C) during the PCV morphogenesis. I found that cv-c mediates PCV morphogenesis downstream of BMP signal by inactivating various Rho-type small GTPases. Interestingly, I found that cv-c is also required for Dpp transport, while Sog/Cv mediated BMP signal is guided at the ectopic wing veins caused by loss of Rho-type small GTPases. These observations identified a feed-forward mechanism coupling Dpp transport and PCV morphogenesis. To address how BMP signal specifies diversified insect wing vein patterns, I then introduced sawfly as a new model. I found that dpp is ubiquitously expressed but BMP signal reflects distinct fore- and hindwing vein patterns in sawfly. To address if Dpp transport mechanism is involved in wing vein formation, Cv/Tsg was identified from sawfly. Loss of dpp or cv/tsg by RNAi affected BMP signal and all of wing venations. These observations suggest that ubiquitously expressed Dpp is redistributed to specify distinct fore- and hindwing vein patterns in sawfly. Taken together, I found that the extracellular distribution of Dpp/BMP is tightly regulated and coordinated to achieve precise patterning and morphogenesis of the insect wing veins. Furthermore, this study raises an interesting possibility that changes in the directionality of Dpp/BMP diffusion may underlie distinct insect wing vein patterns.

Evoluution aikana konservoituneet…

Subjects/Keywords: developmental biology; developmental biology

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APA (6^th Edition):

Matsuda, S. (2013). The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/40121

Chicago Manual of Style (16^th Edition):

Matsuda, Shinya. “The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing.” 2013. Doctoral Dissertation, University of Helsinki. Accessed December 08, 2019. http://hdl.handle.net/10138/40121.

MLA Handbook (7^th Edition):

Matsuda, Shinya. “The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing.” 2013. Web. 08 Dec 2019.

Vancouver:

Matsuda S. The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing. [Internet] [Doctoral dissertation]. University of Helsinki; 2013. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/10138/40121.

Council of Science Editors:

Matsuda S. The Extracellular Regulation of Bone Morphogenetic Proteins in Drosophila and Sawfly Wing. [Doctoral Dissertation]. University of Helsinki; 2013. Available from: http://hdl.handle.net/10138/40121

University of Helsinki

2. Veistinen, Lotta. Role of Gli3 during intramembranous calvarial bone development.

Degree: Institute of Dentistry, Orthodontics, 2015, University of Helsinki

URL: http://hdl.handle.net/10138/154665

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The flat bones of the skull, the calvarial bones, develop by intramembranous ossification during which mesenchymal cells first condense and subsequently differentiate into osteoblasts. Sutures… (more)

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The flat bones of the skull, the calvarial bones, develop by intramembranous ossification during which mesenchymal cells first condense and subsequently differentiate into osteoblasts. Sutures separate the calvarial bones and facilitate the synchronized growth of the underlying brain and the calvaria. Hedgehog (Hh) signalling has an indisputable role in craniofacial development as well as during endochondral ossification. Yet, little is known about its function during intramembranous ossification of the calvarial bones. GLI-Kruppel family member 3 (Gli3) is a zinc-finger transcription factor that mediates Hh signalling. In the absence of Hh ligand Gli3 is proteolytically cleaved into a repressor that inhibits transcription of Hh target genes. Mutations in GLI3 cause Greig cephalopolysyndactyly syndrome in humans, in which an infrequent, but significant feature is premature fusion of the metopic suture (interfrontal suture in mice). We have used Gli3 loss-of- function mouse (Gli3Xt-J/Xt-J) as a model to investigate the effects of aberrant Hh signalling during calvarial development. In my thesis I describe how loss of Gli3 causes craniosynostosis of the lambdoid as well as interfrontal sutures in mice. Elevated proliferation and ectopic differentiation of osteoprogenitors underlies this phenomenon. We were able to rescue craniosynostosis in these mice by two mechanisms. Firstly, by elevating fibroblast growth factor (Fgf) signalling in the suture prior to its fusion by imbedding Fgf2 soaked beads in tissue culture. This induced Twist1 expression, which inhibits function of ectopically expressed Runx2. Secondly, craniosynostosis was prevented by genetically reducing Runx2 activity by generating Gli3Xt-J/Xt-J;Runx2+/- mice, which normalized elevated levels of Bmp signalling in the affected sutures. We also put forward a model of how Hh signalling helps to maintain the integrity of bone margins during calvarial development. The repressor isoform of Gli3 inhibits Runx2 activity in the early osteoprogenitor cells. Runx2, on the other hand, activates Ihh expression in the mature osteoblasts, which then induces osteogenesis by inhibiting the function of Gli3 repressor. Our findings indicate that Gli3 and Hh signalling have an important role in mediating the location of osteoblast differentiation and the speed of bone formation in the developing calvaria. Uncovering the cellular and molecular mechanisms that underlie normal calvarial development, as well as pathological processes, is a vital step in developing treatment strategies for patients with craniosynostosis.

Kallon litteät peitinluut kehittyvät ns. suoran luutumisen mekanismilla eli intramembranoottisesti: mesenkymaaliset solut muodostavat ensin tiivistymän, jonka jälkeen ne erilaistuvat asteittain luusoluiksi eli osteoblasteiksi. Kasvavia kallon peitinluita erottavat kallon saumat, joissa luiden aktiivinen kasvu jatkuu mahdollistaen alla olevien aivojen samanaikaisen kasvun. Kraniosynostoosi on patologinen tila, jossa yksi tai useampi kallon sauma luutuu…

Subjects/Keywords: developmental biology; developmental biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Veistinen, L. (2015). Role of Gli3 during intramembranous calvarial bone development. (Doctoral Dissertation). University of Helsinki. Retrieved from http://hdl.handle.net/10138/154665

Chicago Manual of Style (16^th Edition):

Veistinen, Lotta. “Role of Gli3 during intramembranous calvarial bone development.” 2015. Doctoral Dissertation, University of Helsinki. Accessed December 08, 2019. http://hdl.handle.net/10138/154665.

MLA Handbook (7^th Edition):

Veistinen, Lotta. “Role of Gli3 during intramembranous calvarial bone development.” 2015. Web. 08 Dec 2019.

Vancouver:

Veistinen L. Role of Gli3 during intramembranous calvarial bone development. [Internet] [Doctoral dissertation]. University of Helsinki; 2015. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/10138/154665.

Council of Science Editors:

Veistinen L. Role of Gli3 during intramembranous calvarial bone development. [Doctoral Dissertation]. University of Helsinki; 2015. Available from: http://hdl.handle.net/10138/154665

Wright State University

3. Barrios, Erick A. Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain.

Degree: MS, Physiology and Neuroscience, 2017, Wright State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

► We used the chicken embryo model to examine whether altered incubation temperature during chicken embryo development would alter the protein expression of heat shock protein… (more)

Subjects/Keywords: Developmental Biology; developmental biology

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APA (6^th Edition):

Barrios, E. A. (2017). Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain. (Masters Thesis). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

Chicago Manual of Style (16^th Edition):

Barrios, Erick A. “Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain.” 2017. Masters Thesis, Wright State University. Accessed December 08, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006.

MLA Handbook (7^th Edition):

Barrios, Erick A. “Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain.” 2017. Web. 08 Dec 2019.

Vancouver:

Barrios EA. Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain. [Internet] [Masters thesis]. Wright State University; 2017. [cited 2019 Dec 08]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006.

Council of Science Editors:

Barrios EA. Cx43 Expression Increases in Response to Increased Temperature Incubation in the Developing Chicken Embryonic Brain. [Masters Thesis]. Wright State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1502963524023006

4. Kilpatrick, Kaylon Ann. Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster.

Degree: 2016, Mississippi College

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10128977

► Polycystic Ovarian Syndrome (PCOS) is a metabolic and endocrine disorder that is the most common cause of infertility. PCOS can manifest itself as a… (more)

Subjects/Keywords: Biology; Developmental biology

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APA (6^th Edition):

Kilpatrick, K. A. (2016). Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster. (Thesis). Mississippi College. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10128977

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kilpatrick, Kaylon Ann. “Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster.” 2016. Thesis, Mississippi College. Accessed December 08, 2019. http://pqdtopen.proquest.com/#viewpdf?dispub=10128977.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kilpatrick, Kaylon Ann. “Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster.” 2016. Web. 08 Dec 2019.

Vancouver:

Kilpatrick KA. Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster. [Internet] [Thesis]. Mississippi College; 2016. [cited 2019 Dec 08]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10128977.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kilpatrick KA. Starvation induces Polycystic Ovarian Syndrome (PCOS) like symptoms in Drosophila melanogaster. [Thesis]. Mississippi College; 2016. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10128977

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Singh, Nirupama Janardansingh. Identification and characterization of novel homodimer targets of CeTwist.

Degree: PhD, Biology, 2012, The Catholic University of America

URL: http://hdl.handle.net/1961/10286

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Degree awarded: Ph.D. Biology. The Catholic University of America

Twist, a basic-helix-loop-helix (bHLH) transcription factor, functions in the mesoderm in many organisms. bHLH transcription factors… (more)

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Degree awarded: Ph.D. Biology. The Catholic University of America

Twist, a basic-helix-loop-helix (bHLH) transcription factor, functions in the mesoderm in many organisms. bHLH transcription factors dimerise with their helix-loop-helix region forming a heterodimer or a homodimer and the basic regions of the dimers bind to a consensus sequence known as an E box on their target genes. Previous evidence suggests a distinct role for CeTwist homodimers and heterodimers (with CeE/DA) in different mesoderm cells in the model organism C. elegans. We have already identified a number of CeTwist heterodimer targets. However, no CeTwist homodimer targets have been identified to date. The aim of this project is to discover and study such genes to understand bHLH dimer-specific transcriptional regulation. To find CeTwist homodimer targets, we overexpressed CeTwist by using an inducible heat shock promoter and found the potential target genes that are overexpressed using a global gene expression measurement technique called Affymetrix oligonucleotide microarrays. Nineteen genes from a list of overexpressed genes were prioritized based on higher microarray value. Using transcriptional GFP reporters, we examined the expression pattern of these genes. We were interested in a pattern that may partially overlap with the CeTwist expression pattern, and we found five genes in this category. Then GFP expression was studied in mutant animals of the hlh-2 (CeE/DA) and hlh-8 (CeTwist) genes for further validation. The results suggest that we found one CeTwist homodimer target, two targets of CeTwist and CeE/DA heterodimers and two more CeTwist targets that need further characterization. Next, we studied the promoter region of the new CeTwist targets by using 5'deletion reporter constructs. In one of the heterodimer targets, we found a previously identified E box playing a role in target gene expression whereas for the homodimer target, we identified a palindromic sequence CACGTG that plays an important role in expression. This interesting result is consistent with bHLH homodimers preferentially binding to palindromic sequences with identical half-sites for regulation. Moreover, we also found that two of the new CeTwist target genes identified in this study, have human homologues which are mutated in human diseases. Therefore, understanding the transcriptional regulation of these genes is important.

Made available in DSpace on 2012-06-01T16:44:28Z (GMT). No. of bitstreams: 1 Singh_cua_0043A_10331display.pdf: 2087461 bytes, checksum: dc2a8c207c9a1b35c0f3ccfc0942a714 (MD5)

Advisors/Committee Members: Corsi, Ann K (Advisor), Golin, John E (Other), Tuma, Pamela L (Other).

Subjects/Keywords: Biology; Developmental biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Singh, N. J. (2012). Identification and characterization of novel homodimer targets of CeTwist. (Doctoral Dissertation). The Catholic University of America. Retrieved from http://hdl.handle.net/1961/10286

Chicago Manual of Style (16^th Edition):

Singh, Nirupama Janardansingh. “Identification and characterization of novel homodimer targets of CeTwist.” 2012. Doctoral Dissertation, The Catholic University of America. Accessed December 08, 2019. http://hdl.handle.net/1961/10286.

MLA Handbook (7^th Edition):

Singh, Nirupama Janardansingh. “Identification and characterization of novel homodimer targets of CeTwist.” 2012. Web. 08 Dec 2019.

Vancouver:

Singh NJ. Identification and characterization of novel homodimer targets of CeTwist. [Internet] [Doctoral dissertation]. The Catholic University of America; 2012. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/1961/10286.

Council of Science Editors:

Singh NJ. Identification and characterization of novel homodimer targets of CeTwist. [Doctoral Dissertation]. The Catholic University of America; 2012. Available from: http://hdl.handle.net/1961/10286

University of California – San Francisco

6. Saleem, Arif Hussain. The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development.

Degree: Biomedical Sciences, 2013, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/9x9433d6

► The Adhesion family of G protein-coupled receptors (AdGCPRs) is a unique set of cell-surface receptors with relatively unknown signaling capabilities and physiological functions. Previous work… (more)

▼ The Adhesion family of G protein-coupled receptors (AdGCPRs) is a unique set of cell-surface receptors with relatively unknown signaling capabilities and physiological functions. Previous work has revealed physiological roles for some AdGPCRs, ranging from orchestrating planar cell polarity in Drosophila epithelium to mediating cell-extracellular matrix interactions in the developing central nervous system. Mutations in some AdGPCRs have been linked to human disease. However, most AdGPCRs remain orphans, with unidentified binding partners, unclear physiological roles, and unknown downstream signaling. Eltd1 (also referred to as ETL) is a member of the AdGPCR subfamily known to be expressed in cardiovascular tissues including heart and blood vessels. I performed morpholino-mediated knockdown of eltd1 in zebrafish to elucidate its physiological function and attempt to gain insight into binding partners and putative signaling pathways. I found that knockdown of eltd1 in zebrafish produced a defect in the establishment of left-right (LR) asymmetry as well as the processes of convergence and extension. Using targeted morpholino injections and fluorescent timelapse microscopy, I was able to show that loss of eltd1 disrupts nodal flow in Kuppfer's vesicle, the organ responsible for establishing LR asymmetry in zebrafish. Interestingly, the early developmental phenotypes associated with eltd1 knockdown are similar to those often observed in morphants and mutants of various planar cell polarity (PCP) genes. Eltd1 morphants also displayed defects in cardiovascular development, consistent with expression data from mouse and rat that localize eltd1 transcripts most strongly to heart and blood vessels. I sought to characterize this phenotype and implement cell-culture assays to visualize Eltd1 in primary and cultured cells. Finally, I developed a strategy to isolate N-terminal binding partners of Eltd1. This work provides a foundation for further investigation of candidate Eltd1 binding partners and putative downstream signaling pathways.

Subjects/Keywords: Biology; Developmental biology

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APA (6^th Edition):

Saleem, A. H. (2013). The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/9x9433d6

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Saleem, Arif Hussain. “The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development.” 2013. Thesis, University of California – San Francisco. Accessed December 08, 2019. http://www.escholarship.org/uc/item/9x9433d6.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Saleem, Arif Hussain. “The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development.” 2013. Web. 08 Dec 2019.

Vancouver:

Saleem AH. The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development. [Internet] [Thesis]. University of California – San Francisco; 2013. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/9x9433d6.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Saleem AH. The Role of Zebrafish Adhesion GPCR Eltd1 in Left-Right Asymmetry and Cardiovascular Development. [Thesis]. University of California – San Francisco; 2013. Available from: http://www.escholarship.org/uc/item/9x9433d6

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Bala, Neeta. AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila.

Degree: 2017, Icahn School of Medicine at Mount Sinai

URL: http://pqdtopen.proquest.com/#viewpdf?dispub=10637108

► Cell signaling determines cellular behavior through the regulation of complex biochemical networks, slight disruptions in which can lead to a plethora of pathologies. The… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Bala, N. (2017). AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila. (Thesis). Icahn School of Medicine at Mount Sinai. Retrieved from http://pqdtopen.proquest.com/#viewpdf?dispub=10637108

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bala, Neeta. “AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila.” 2017. Thesis, Icahn School of Medicine at Mount Sinai. Accessed December 08, 2019. http://pqdtopen.proquest.com/#viewpdf?dispub=10637108.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bala, Neeta. “AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila.” 2017. Web. 08 Dec 2019.

Vancouver:

Bala N. AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila. [Internet] [Thesis]. Icahn School of Medicine at Mount Sinai; 2017. [cited 2019 Dec 08]. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10637108.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bala N. AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila. [Thesis]. Icahn School of Medicine at Mount Sinai; 2017. Available from: http://pqdtopen.proquest.com/#viewpdf?dispub=10637108

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Temple University

8. Nama, Kaushik. Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development.

Degree: PhD, 2015, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,360057

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Biology

The Wnt signaling pathway is highly conserved in metazoan animals. Wnt signaling regulates an array of cellular processes that include motility, polarity, cell fate… (more)

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Biology

The Wnt signaling pathway is highly conserved in metazoan animals. Wnt signaling regulates an array of cellular processes that include motility, polarity, cell fate determination, primary axis formation and organogenesis and recently have been implicated in stem cell renewal. Deregulated Wnt signaling has tragic consequences for the developing embryo and is a causative factor for a number of pleiotropic human pathologies such as cancers of the breast, colon and skin, skeletal defects and human birth defect disorders including the most common human neural tube closure defect: spina bifida. Modulation of the actin cytoskeleton via the non-canonical Wnt signaling pathway mediate cell polarity and cell migration that are required for proper vertebrate gastrulation and subsequent neurulation. However, the mechanism(s) by how the non-canonical pathway mediates this actin cytoskeleton modulation is not fully understood. Identifying and characterizing novel signaling components of the non-canonical pathway remain essential to understand its role during embryogenesis. The Formin-homology protein Dishevelled associated activator of morphogenesis 1 (Daam1) was previously characterized as an essential component for non-canonical Wnt-dependent regulation of cytoskeletal reorganization and cell migration. Daam1 and Daam2 are members of the Daam family of proteins but the role of Daam2 in early embryonic development however remains conflicting as to whether it functions in the canonical or non-canonical Wnt signaling pathway. In this thesis, I cloned and functionally characterized the role of Xenopus Daam2 in the Wnt signaling pathway. Co-immunoprecipitation assays confirm the binding of Daam2 with Dishevelled (Dvl) as well as the domains within those proteins required for interaction. Interestingly the binding of Daam2 and Dvl was subject to Wnt regulation. Sub-cellular localization immunofluorescence studies using mammalian cell culture system reveal Daam2 is cytoplasmic and regulates the actin cytoskeleton by modulating the actin filament formation. In Xenopus embryos, Daam2 is temporally expressed at very low levels maternally and its expression increases during neurulation and remains subsequently elevated after neurulation. Daam2 expression is spatially refined to areas of highly dynamic actin reorganization such as mesoderm, notochord and neural tube. The knockdown of Daam2 in Xenopus embryos specifically produces neural tube closure defect suggesting a role in non-canonical signaling. Indeed studies examining the role of Daam2 in canonical Wnt signaling found no role for this protein in canonical Wnt signaling. These studies taken together identify Daam2 as an important Wnt signaling component that functions in the non-canonical Wnt signaling pathway and regulates neural tube morphogenesis.

Temple University – Theses

Advisors/Committee Members: Habas, Raymond;, Gruberg, Edward R., Balciunas, Darius, Runnels, Loren W.;.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Nama, K. (2015). Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,360057

Chicago Manual of Style (16^th Edition):

Nama, Kaushik. “Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development.” 2015. Doctoral Dissertation, Temple University. Accessed December 08, 2019. http://digital.library.temple.edu/u?/p245801coll10,360057.

MLA Handbook (7^th Edition):

Nama, Kaushik. “Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development.” 2015. Web. 08 Dec 2019.

Vancouver:

Nama K. Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development. [Internet] [Doctoral dissertation]. Temple University; 2015. [cited 2019 Dec 08]. Available from: http://digital.library.temple.edu/u?/p245801coll10,360057.

Council of Science Editors:

Nama K. Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development. [Doctoral Dissertation]. Temple University; 2015. Available from: http://digital.library.temple.edu/u?/p245801coll10,360057

Purdue University

9. Hilger, Allison Ilice. New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering.

Degree: MS, Speech, Language, and Hearing Sciences, 2015, Purdue University

URL: http://docs.lib.purdue.edu/open_access_theses/503

► Stuttering is a disorder that involves a breakdown in the speech-motor system, resulting in disfluencies such as part- and whole-word repetitions, prolongations, and blocks.… (more)

Subjects/Keywords: Developmental Biology

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APA (6^th Edition):

Hilger, A. I. (2015). New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering. (Thesis). Purdue University. Retrieved from http://docs.lib.purdue.edu/open_access_theses/503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hilger, Allison Ilice. “New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering.” 2015. Thesis, Purdue University. Accessed December 08, 2019. http://docs.lib.purdue.edu/open_access_theses/503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hilger, Allison Ilice. “New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering.” 2015. Web. 08 Dec 2019.

Vancouver:

Hilger AI. New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering. [Internet] [Thesis]. Purdue University; 2015. [cited 2019 Dec 08]. Available from: http://docs.lib.purdue.edu/open_access_theses/503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hilger AI. New evidence that bimanual motor timing performance is not a significant factor in developmental stuttering. [Thesis]. Purdue University; 2015. Available from: http://docs.lib.purdue.edu/open_access_theses/503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hawaii – Manoa

10. Tom, Sasa K. Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120.

Degree: 2015, University of Hawaii – Manoa

URL: http://hdl.handle.net/10125/100363

►

Ph.D. University of Hawaii at Manoa 2014.

A central paradigm in developmental biology concerns the differentiation of cells despite the fundamental sameness of the genetic… (more)

▼

Ph.D. University of Hawaii at Manoa 2014.

A central paradigm in developmental biology concerns the differentiation of cells despite the fundamental sameness of the genetic complement shared by all cells in the organism. The filamentous cyanobacterium Anabaena sp. strain PCC 7120 is an ideal model system for the study of development. In response to nitrogen deprivation, Anabaena differentiates nitrogen-fixing heterocyst cells in a periodic pattern. Anabaena research has practical applications in human health, sustainable agriculture, and biofuel production. This study aimed to characterize novel interactions to refine the current understanding of Anabaena development. Investigations were performed to elucidate the hetF-dependent activation of differentiation. A novel genetic regulatory network involving hetF, a CHF class protease, and the negative regulator patU3 and other developmental genes was identified. A component of the hetZ-patU5-patU3 gene cluster, PatU3 was shown to directly interact with HetZ, another activator of differentiation. Genetic epistasis analysis determined that PatU3 suppressed positive regulation by HetZ and HetR. These negative feedback loops explain the elevated HetR-GFP concentrations in hetF-dependent strains despite the paradoxical absence of heterocysts. The HetF-dependent pathway may act as a control point prior to commitment to the heterocyst cell fate. Lateral inhibition by PatS and HetN, which both contain the same RGSGR pentapeptide sequence, involves regulation by HetR. The unique domains present in the structure of HetR may relate to its activity as a transcriptional activator. In this study, genetic and cytological approaches were used to identify residues in HetR necessary for interaction with PatS, HetN and RGSGR. A related investigation demonstrated that the RGSGR-pentapeptide derived from HetN directs pattern formation by direct cytoplasmic exchange. Lastly, protein phosphorylation plays a prominent role in varied biological processes. The PP2C-type protein phosphatase All1758 was characterized in this study. The corresponding all1758 gene is controlled by the developmental genes ntcA and hetR. All1758 affects later stages of differentiation and may represent a critical link between cell growth, cell division and morphogenesis by controlling putative sigma factor regulators (antisigma factors and antisigma factor agonists) and cell division genes (including FtsZ and MinCE). Taken together, these findings support additional regulatory mechanisms necessary for proper Anabaena development.

Subjects/Keywords: developmental biology

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APA (6^th Edition):

Tom, S. K. (2015). Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tom, Sasa K. “Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120.” 2015. Thesis, University of Hawaii – Manoa. Accessed December 08, 2019. http://hdl.handle.net/10125/100363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tom, Sasa K. “Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120.” 2015. Web. 08 Dec 2019.

Vancouver:

Tom SK. Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/10125/100363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tom SK. Role of hetF and patU3 in the regulation of heterocyst development in Anabaena sp. Strain pcc 7120. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/100363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

11. Gu, Jinmo. An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.

Degree: PhD, Molecular, Cellular and Developmental Biology, 2016, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1458152802

► Skeletal muscle growth immediately following birth is a critical stage of development needed for proper body posture and locomotion. However, compared to embryogenesis and adulthood,… (more)

Subjects/Keywords: Developmental Biology

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APA (6^th Edition):

Gu, J. (2016). An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1458152802

Chicago Manual of Style (16^th Edition):

Gu, Jinmo. “An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.” 2016. Doctoral Dissertation, The Ohio State University. Accessed December 08, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1458152802.

MLA Handbook (7^th Edition):

Gu, Jinmo. “An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates.” 2016. Web. 08 Dec 2019.

Vancouver:

Gu J. An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates. [Internet] [Doctoral dissertation]. The Ohio State University; 2016. [cited 2019 Dec 08]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1458152802.

Council of Science Editors:

Gu J. An NF-kappaB - EphrinA5 - Dependent Communication between NG2+ Interstitial Cells and Myoblasts Promotes Muscle Growth in Neonates. [Doctoral Dissertation]. The Ohio State University; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1458152802

University of California – San Diego

12. Knight, Hannah. Patterning of the cardiac inflow tract in zebrafish.

Degree: Biology, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/2354t8g4

► The mature heart is comprised of multiple types of specialized cardiomyocytes, each with distinct functional attributes. However, the mechanisms that specify discrete populations of cardiac… (more)

▼ The mature heart is comprised of multiple types of specialized cardiomyocytes, each with distinct functional attributes. However, the mechanisms that specify discrete populations of cardiac progenitors are not well understood. For example, it is clear that cardiac pacemaking activity is confined to a specialized population of cells in the cardiac venous pole, but the signals that create the appropriate number of pacemaker cardiomyocytes remain unknown. We have therefore sought to understand how pacemaker cells develop in the zebrafish embryo. First, we have investigated pacemaker cells in the inflow tract (IFT) of wild-type zebrafish embryos. We have observed that IFT cardiomyocytes express a suite of molecular markers that are reminiscent of mammalian pacemaker cells and that confer attributes specific to this population. Furthermore, we have determined that IFT progenitors are localized to discrete areas at the edges of the heart fields, prior to their differentiation. Next, we have shown that the specification of this IFT progenitor population is influenced by opposing inputs from two signaling pathways: Hedgehog (Hh) signaling and Bmp signaling. Given our prior finding that Hh signaling promotes cardiomyocyte production, we were surprised to discover that Hh signaling also acts to delimit the number of IFT cardiomyocytes. Using both genetic and pharmacological manipulations of the Hh pathway, we have shown that loss of Hh signaling results in dramatically expanded expression of IFT markers. Conversely, Bmp signaling drives IFT formation, as embryos with reduced Bmp signaling have a diminished IFT. Timed manipulations of Hh and Bmp activity have demonstrated that both signals act during early steps of cardiac patterning to define IFT size. Intriguingly, reducing both Hh and Bmp signaling restores a nearly normal number of IFT cells. We therefore propose a model in which IFT specification relies on both limited amounts of Hh signaling and robust levels of Bmp signaling, which together set appropriate boundaries for the IFT progenitor population. Our findings reveal novel mechanisms of cardiac patterning; in the long term, these studies could contribute to our understanding of congenital heart disease and improve efforts to generate pacemaker cells in vitro for use in regenerative medicine.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Knight, H. (2017). Patterning of the cardiac inflow tract in zebrafish. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/2354t8g4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Knight, Hannah. “Patterning of the cardiac inflow tract in zebrafish.” 2017. Thesis, University of California – San Diego. Accessed December 08, 2019. http://www.escholarship.org/uc/item/2354t8g4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Knight, Hannah. “Patterning of the cardiac inflow tract in zebrafish.” 2017. Web. 08 Dec 2019.

Vancouver:

Knight H. Patterning of the cardiac inflow tract in zebrafish. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/2354t8g4.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Knight H. Patterning of the cardiac inflow tract in zebrafish. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/2354t8g4

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

13. Luong, Mui Nhuc. The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis.

Degree: Biological Sciences, 2014, University of California – Irvine

URL: http://www.escholarship.org/uc/item/7cj5n71d

► Understanding the molecular mechanisms that govern animal development is one of the major challenges in developmental biology. The TGF-β members such as the bone morophogenetic… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Luong, M. N. (2014). The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/7cj5n71d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Luong, Mui Nhuc. “The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis.” 2014. Thesis, University of California – Irvine. Accessed December 08, 2019. http://www.escholarship.org/uc/item/7cj5n71d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Luong, Mui Nhuc. “The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis.” 2014. Web. 08 Dec 2019.

Vancouver:

Luong MN. The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis. [Internet] [Thesis]. University of California – Irvine; 2014. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/7cj5n71d.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Luong MN. The Regulation of BMP Signaling in Mouse Embryonic Stem Cells and Early Mouse Embryogenesis. [Thesis]. University of California – Irvine; 2014. Available from: http://www.escholarship.org/uc/item/7cj5n71d

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

14. Lu, Fei. Transcriptional Regulation of Heart Development and Function.

Degree: Molec, Cell, & Dev Biology, 2016, UCLA

URL: http://www.escholarship.org/uc/item/4kt1t2dx

► Cardiac morphogenesis and the maintenance of cardiac physiology require complex and well-orchestrated cardiac transcription programs. Misregulation of cardiac transcription programs leads to severe developmental defects,… (more)

▼ Cardiac morphogenesis and the maintenance of cardiac physiology require complex and well-orchestrated cardiac transcription programs. Misregulation of cardiac transcription programs leads to severe developmental defects, and is associated with human congenital heart diseases (CHD). To better understand the transcription program governing normal heart development, my research focused specifically on two essential genes for vertebrate heart development: tbx20 that encodes a cardiac transcription factor and rtf1 that encodes a multifunctional transcription regulatory protein. First, I reported the identification of a zebrafish tbx20 null mutation that causes severe cardiac progenitor defects, highlighting a previously unappreciated role for Tbx20 in promoting vertebrate cardiogenesis. I also revealed a novel function of Tbx20 in enhancing cardiomyocyte proliferation as a transcriptional activator. Second, I found that loss of Rtf1, a RNA polymerase II associated factor 1 (PAF1C) complex component, suppresses the cardiac transcription program and prevents the formation of cardiac progenitors. I discovered that Rtf1 controls cardiac progenitor formation and heart tube morphogenesis via two independent mechanisms: Rtf1 pushes multi-potent mesodermal cells to a cardiac fate through activating the cardiac transcription program and Rtf1 supports cardiomyocyte differentiation and heart tube morphogenesis by modulating the epigenome. Lastly, I found that cardiomyocyte-specific ablation of rtf1 in adult mouse heart leads to dilated cardiomyopathy and heart failure. Expression of genes encoding lipid metabolism related proteins, myofibrillar proteins, mitochondrial proteins and ion channels were significantly downregulated in rtf1-deficent hearts, suggesting that Rtf1 is an important regulator of the cardiac gene program that maintains myofibril integrity and cardiac function. Taken together, my studies provide new insights into the regulation of cardiac transcription program in developing and mature myocardium, and presents new candidate genes for CHD and cardiac dysfunction in humans.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Lu, F. (2016). Transcriptional Regulation of Heart Development and Function. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/4kt1t2dx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lu, Fei. “Transcriptional Regulation of Heart Development and Function.” 2016. Thesis, UCLA. Accessed December 08, 2019. http://www.escholarship.org/uc/item/4kt1t2dx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lu, Fei. “Transcriptional Regulation of Heart Development and Function.” 2016. Web. 08 Dec 2019.

Vancouver:

Lu F. Transcriptional Regulation of Heart Development and Function. [Internet] [Thesis]. UCLA; 2016. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/4kt1t2dx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lu F. Transcriptional Regulation of Heart Development and Function. [Thesis]. UCLA; 2016. Available from: http://www.escholarship.org/uc/item/4kt1t2dx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

15. Pradhan, Arjana. Signaling pathways promoting chamber identity in the zebrafish heart.

Degree: Biology, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/52d3t9nz

► The atrial and ventricular chambers of the heart behave as distinctfunctional subunits with unique morphological, contractile, and electrophysiological properties. Therefore, the proper differentiation of atrial… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Pradhan, A. (2016). Signaling pathways promoting chamber identity in the zebrafish heart. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/52d3t9nz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pradhan, Arjana. “Signaling pathways promoting chamber identity in the zebrafish heart.” 2016. Thesis, University of California – San Diego. Accessed December 08, 2019. http://www.escholarship.org/uc/item/52d3t9nz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pradhan, Arjana. “Signaling pathways promoting chamber identity in the zebrafish heart.” 2016. Web. 08 Dec 2019.

Vancouver:

Pradhan A. Signaling pathways promoting chamber identity in the zebrafish heart. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/52d3t9nz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pradhan A. Signaling pathways promoting chamber identity in the zebrafish heart. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/52d3t9nz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

16. Flores, Michelle. Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot.

Degree: Biology, 2017, University of California – San Diego

URL: http://www.escholarship.org/uc/item/1332h5gd

► The limb is one of the most evolutionarily diverse structures in vertebrate morphology. Our lab focuses on the lesser Egyptian jerboa, Jaculus jaculus, as an… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Flores, M. (2017). Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/1332h5gd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Flores, Michelle. “Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot.” 2017. Thesis, University of California – San Diego. Accessed December 08, 2019. http://www.escholarship.org/uc/item/1332h5gd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Flores, Michelle. “Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot.” 2017. Web. 08 Dec 2019.

Vancouver:

Flores M. Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot. [Internet] [Thesis]. University of California – San Diego; 2017. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/1332h5gd.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Flores M. Investigating Neuro-Muscular Junction Formation During Naturally Occurring Muscle Loss in the Neonatal Jerboa Foot. [Thesis]. University of California – San Diego; 2017. Available from: http://www.escholarship.org/uc/item/1332h5gd

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

17. Alvarez, Maribel. The role of Myoglianin in Drosophila metamorphosis.

Degree: Biological Sciences, 2015, University of California – Irvine

URL: http://www.escholarship.org/uc/item/6jt9f5kf

► Signaling by the Transforming Growth Factor-Beta; (TGF-Beta) superfamily is essential for many cellular processes and is required throughout the life of many organisms. TGF-Beta; signaling… (more)

▼ Signaling by the Transforming Growth Factor-Beta; (TGF-Beta) superfamily is essential for many cellular processes and is required throughout the life of many organisms. TGF-Beta; signaling triggers distinct developmental programs and cellular processes, thus disruption of the pathway is associated with defects in development and growth of the organism. Signaling specificity is achieved through the use of distinct pathway components and combinatorial action with other cell signaling pathways. The main goal of my thesis project was to understand the roles of two ligands of the TGF-Beta; superfamily, Maverick (Mav) and Myoglianin (Myo), and their mechanism of action in Drosophila. We first characterized several mutant alleles for mav and myo that were previously generated in the lab. Next, we investigated the role of mav at the neuromuscular junction (NMJ). Loss-of-function analysis suggests that mav has no affect on growth of the NMJ. However, ectopic expression of mav in glial cells does have an affect on the NMJ, suggesting that while not essential, NMJ growth may be sensitive to changes in levels of Mav. Phenotypic analysis of mutations in myo that encodes an activin ligand, revealed a wide range of metamorphosis phenotypes reminiscent of loss of the steroid hormone ecdysone. We investigated the role of myo in several tissues that undergo specific changes during metamorphosis. We determined that myo is selectively required for expression of the ecdysone receptor EcR-B1 isoform in a diverse group of neurons in the central nervous system, but not in other EcR-B1 expressing tissues, for example gut, fat body and salivary glands. Therefore one of the roles of Myo is to regulate the ability of specific cells to respond to ecdysone. Importantly, we found that salivary gland destruction in response to ecdysone signaling is dependent on activin signaling, but this occurs through regulation of EcR-B1 in specific cells in the brain, rather than in the salivary gland itself. Our data argue for a model in which ecdysone signaling utilizes both direct and indirect inputs to coordinate salivary gland destruction during pupal metamorphosis. The work presented here provides insights into the requirement of Myo/activin signaling in regulation and coordination of steroid dependent biological processes in animals.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Alvarez, M. (2015). The role of Myoglianin in Drosophila metamorphosis. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/6jt9f5kf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alvarez, Maribel. “The role of Myoglianin in Drosophila metamorphosis.” 2015. Thesis, University of California – Irvine. Accessed December 08, 2019. http://www.escholarship.org/uc/item/6jt9f5kf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alvarez, Maribel. “The role of Myoglianin in Drosophila metamorphosis.” 2015. Web. 08 Dec 2019.

Vancouver:

Alvarez M. The role of Myoglianin in Drosophila metamorphosis. [Internet] [Thesis]. University of California – Irvine; 2015. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/6jt9f5kf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alvarez M. The role of Myoglianin in Drosophila metamorphosis. [Thesis]. University of California – Irvine; 2015. Available from: http://www.escholarship.org/uc/item/6jt9f5kf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

18. Aghajanian, Patrick. The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster.

Degree: Molec, Cell, & Dev Biology, 2014, UCLA

URL: http://www.escholarship.org/uc/item/9jk7n6xr

► Stem cell maintenance in the small intestine in vertebrates has been well studied, though many factors involved in that process are still unknown. This study… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Aghajanian, P. (2014). The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/9jk7n6xr

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aghajanian, Patrick. “The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster.” 2014. Thesis, UCLA. Accessed December 08, 2019. http://www.escholarship.org/uc/item/9jk7n6xr.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aghajanian, Patrick. “The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster.” 2014. Web. 08 Dec 2019.

Vancouver:

Aghajanian P. The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster. [Internet] [Thesis]. UCLA; 2014. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/9jk7n6xr.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aghajanian P. The Intestinal Visceral Musculature: A Dynamic Niche for Metamorphosing Midgut and Hindgut of Drosophila melanogaster. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/9jk7n6xr

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

19. Mazzoni, Jenna Therese. The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina.

Degree: Biological Sciences, 2016, University of California – Irvine

URL: http://www.escholarship.org/uc/item/64w403wz

► Coordinating angiogenesis with the acquisition of specialized endothelial cell properties is essential for proper vascular function. Within the retina, endothelial cells form a blood- retina… (more)

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Mazzoni, J. T. (2016). The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/64w403wz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mazzoni, Jenna Therese. “The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina.” 2016. Thesis, University of California – Irvine. Accessed December 08, 2019. http://www.escholarship.org/uc/item/64w403wz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mazzoni, Jenna Therese. “The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina.” 2016. Web. 08 Dec 2019.

Vancouver:

Mazzoni JT. The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina. [Internet] [Thesis]. University of California – Irvine; 2016. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/64w403wz.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mazzoni JT. The Wnt inhibitor Apcdd1 controls vascular remodeling and barrier properties of blood vessels in the developing retina. [Thesis]. University of California – Irvine; 2016. Available from: http://www.escholarship.org/uc/item/64w403wz

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Sachani, Saqib. Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development.

Degree: MS, Biological Sciences, 2011, National Library of Canada

URL: http://scholar.uwindsor.ca/etd/299

► Xenopus eye field transcription factors display a dynamic and overlapping expression pattern but their signaling hierarchy is unclear. Current signaling models are inconsistent with regard… (more)

Subjects/Keywords: Developmental Biology.

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APA (6^th Edition):

Sachani, S. (2011). Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development. (Masters Thesis). National Library of Canada. Retrieved from http://scholar.uwindsor.ca/etd/299

Chicago Manual of Style (16^th Edition):

Sachani, Saqib. “Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development.” 2011. Masters Thesis, National Library of Canada. Accessed December 08, 2019. http://scholar.uwindsor.ca/etd/299.

MLA Handbook (7^th Edition):

Sachani, Saqib. “Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development.” 2011. Web. 08 Dec 2019.

Vancouver:

Sachani S. Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development. [Internet] [Masters thesis]. National Library of Canada; 2011. [cited 2019 Dec 08]. Available from: http://scholar.uwindsor.ca/etd/299.

Council of Science Editors:

Sachani S. Role of Six3 and Pax6 in regulating the gene networks involved in vertebrate eye development. [Masters Thesis]. National Library of Canada; 2011. Available from: http://scholar.uwindsor.ca/etd/299

Harvard University

21. Hrvatin, Sinisa. Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells.

Degree: PhD, Biological and Biomedical Sciences, 2012, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433470

► Directed differentiation of human pluripotent stem cells (hPSCs) has the potential to produce human cell types that can be used for disease modeling and cell… (more)

▼ Directed differentiation of human pluripotent stem cells (hPSCs) has the potential to produce human cell types that can be used for disease modeling and cell transplantation. Two key challenges in the differentiation from hPSCs to (β) cells are the specification from pancreatic progenitors to insulin-expressing ((INS⁺ )) cells and the maturation of (INS⁺) cells into glucose responsive β cells. To address the first, two high-content chemical screens identified PKC inhibitors as inducers of (INS⁺) cells from pancreatic progenitors. PKC inhibition generated up to tenfold more (INS⁺) cells while PKC agonists blocked differentiation into (INS⁺) cells. Transplantation of (PKCβ) inhibitor-treated pancreatic progenitors, containing higher proportions of endocrine progenitors and endocrine cells, resulted in mature (β) cells showing higher levels of glucose-stimulated human c-peptide production in vivo. This indicates that in vitro derived (INS⁺) cells might be competent to mature into functional (β) cells. To address the second challenge, we first studied mouse and human (β) cell maturation in vivo. Postnatal mouse (β) cell maturation was marked by an increase in the glucose threshold for insulin secretion and by expression of the gene urocortin 3. To study human (β) cell maturation, a Method for Analyzing RNA following Intracellular Sorting (MARIS) was developed and used for transcriptional profiling of sorted human fetal and adult (β) cells. Surprisingly, transcriptional differences between human fetal and adult (β) cells did not resemble differences between mouse fetal and adult (β) cells, calling into question inter-species homology at the late stages of development. A direct comparison between hPSC-derived (INS⁺) cells, and (β) cells produced during human development is essential to validate directed differentiation and provide a roadmap for maturation of hPSC-derived (INS⁺) cells. Genome-wide transcriptional analysis of sorted (INS⁺) cells derived from three hPSC-lines suggest that different lines produce highly similar (INS⁺) cells, confirming robustness of directed differentiation protocols. Furthermore, nonfunctional hPSC-derived (INS⁺) cells resemble human fetal (β) cells, which are distinct from adult (β) cells. We therefore suggest that in vitro directed differentiation mimics normal human development and reveal differences in gene expression that may account for the functional differences between hPSC-derived (INS⁺) cells and true (β) cells. Advisors/Committee Members: Melton, Douglas A. (advisor), Weir, Gordon (committee member), Eggan, Kevin (committee member), Greiner, Dale (committee member), Zon, Leonard (committee member).

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Hrvatin, S. (2012). Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433470

Chicago Manual of Style (16^th Edition):

Hrvatin, Sinisa. “Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells.” 2012. Doctoral Dissertation, Harvard University. Accessed December 08, 2019. http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433470.

MLA Handbook (7^th Edition):

Hrvatin, Sinisa. “Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells.” 2012. Web. 08 Dec 2019.

Vancouver:

Hrvatin S. Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells. [Internet] [Doctoral dissertation]. Harvard University; 2012. [cited 2019 Dec 08]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433470.

Council of Science Editors:

Hrvatin S. Exploring the Use of Human Pluripotent Stem Cells to Create Functional Pancreatic \(\beta\) Cells. [Doctoral Dissertation]. Harvard University; 2012. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433470

Harvard University

22. Shyer, Amy Elizabeth. The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut.

Degree: PhD, Cell and Developmental Biology, 2013, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11129374

► The vertebrate small intestine is responsible for nutrient absorption during digestion. To this end, the surface area of the gut tube is maximally expanded, both… (more)

▼ The vertebrate small intestine is responsible for nutrient absorption during digestion. To this end, the surface area of the gut tube is maximally expanded, both through a series of loops extending its length and via the development of a complex luminal topography. Here, I first examine the mechanism behind the formation of intestinal loops. I demonstrate that looping morphogenesis is driven by mechanical forces that arise from differential growth between the gut tube and the anchoring dorsal mesenteric sheet. A computational model based on measured parameters not only quantitatively predicts the looping pattern in chick, verifying that these physical forces are sufficient to explain the process, but also accounts for the variation in the gut looping patterns seen in other species. Second, I explore the formation of intestinal villi in chick. I find that intestinal villi form in a stepwise process as a result of physical forces generated as proliferating endodermal and mesenchymal tissues are constrained by sequentially differentiating layers of smooth muscle. A computational model incorporating measured differential growth and the geometric and physical properties of the developing chick gut recapitulates the morphological patterns seen during chick villi formation. I also demonstrate that the same basic biophysical processes underlie the formation of intestinal folds in frog and villi in mice. Finally, I focus on the process by which intestinal stem cells are ultimately localized to the base of each villus. The endoderm expresses the morphogen, Sonic hedgehog (Shh). As the luminal surface of the gut is deformed during villus formation there are resulting local maxima of Shh signaling in the mesenchyme. This results, at high threshold, in the induction of a new signaling center under the villus tip termed the villus cluster. This, in turn, feeds back to restrict proliferating progenitors in the endoderm, the presumptive precursors of the stem cells, to the base of each villus. Together, these studies provide new insight into the formation of the small intestine as a functional organ and highlight the interplay between physical forces, tissue-level growth, and signaling during development. Advisors/Committee Members: Tabin, Clifford James (advisor), Goessling, Wolfram (committee member), Maas, Richard (committee member), Martin, Adam (committee member).

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Shyer, A. E. (2013). The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:11129374

Chicago Manual of Style (16^th Edition):

Shyer, Amy Elizabeth. “The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut.” 2013. Doctoral Dissertation, Harvard University. Accessed December 08, 2019. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11129374.

MLA Handbook (7^th Edition):

Shyer, Amy Elizabeth. “The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut.” 2013. Web. 08 Dec 2019.

Vancouver:

Shyer AE. The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut. [Internet] [Doctoral dissertation]. Harvard University; 2013. [cited 2019 Dec 08]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11129374.

Council of Science Editors:

Shyer AE. The Role of Mechanical Forces in Patterning and Morphogenesis of the Vertebrate Gut. [Doctoral Dissertation]. Harvard University; 2013. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:11129374

Clemson University

23. Ray, Poulomi. EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION.

Degree: PhD, Biological Sciences, 2013, Clemson University

URL: https://tigerprints.clemson.edu/all_dissertations/1121

► This study examines the fundamental principles governing the early stages of skeletal morphogenesis. I specifically investigate the specification of mesenchymal progenitor cells to a skeletal… (more)

▼ This study examines the fundamental principles governing the early stages of skeletal morphogenesis. I specifically investigate the specification of mesenchymal progenitor cells to a skeletal fate. Using explant culture system, I determine that the pharyngeal endoderm was sufficient, but not necessary for specifying pre-chondrogenic identity. FGF signaling is both sufficient and required for specification of Sox9 expression and specification of prechondrogenic identity, as demonstrated by the addition of recombinant FGF protein or the FGF receptor inhibitor (SU5402) to explanted tissue, respectively. However, FGF signaling cannot maintain Sox9 expression or initiate the chondrogenic program as indicated by the absence of Col2a1 transcripts. BMP4 signaling induces and maintains Sox9 expression in the isolated mesenchyme, but only in combination with FGF signaling is capable of inducing Col2a1 expression, and thus, chondrogenesis. I propose that this represents a general mechanism of local signals specifying pre-chondrogenic identity and initiation of the chondrogenic program. Additionally, I determine how specified mesenchymal cells integrate mechanical and molecular information from their environment forming a cartilage condensation. The classical model defines condensation based on increased cell density. Based on our results, we propose a revised model for skeletal condensation, which is based on differential cell shape changes and independent of increasing cell density. By disrupting cytoskeletal reorganization, I demonstrate that tension dependent dynamic cell shape changes drive condensation and modulate the response of the condensing cells to FGF, BMP and TGF-β pathways. Rho Kinase driven actomyosin contractions and myosin II generated differential cell cortex tension drive the cell shape changes and negatively TGF-β pathway. Dorsal and ventral condensations undergo distinct cell shape changes regulated by BMP. This study elucidates the fundamental principles of interplay between mechanical forces and molecular signaling, in a self-organizing system, generating shape and form. Advisors/Committee Members: Chapman, Susan C, Temesvari , Lesly A, Blob , Richard W, Rice , Charles D.

Subjects/Keywords: Developmental Biology

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APA (6^th Edition):

Ray, P. (2013). EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/1121

Chicago Manual of Style (16^th Edition):

Ray, Poulomi. “EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION.” 2013. Doctoral Dissertation, Clemson University. Accessed December 08, 2019. https://tigerprints.clemson.edu/all_dissertations/1121.

MLA Handbook (7^th Edition):

Ray, Poulomi. “EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION.” 2013. Web. 08 Dec 2019.

Vancouver:

Ray P. EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION. [Internet] [Doctoral dissertation]. Clemson University; 2013. [cited 2019 Dec 08]. Available from: https://tigerprints.clemson.edu/all_dissertations/1121.

Council of Science Editors:

Ray P. EARLY SKELETAL MORPHOGENESIS: THE ROLE OF PHARYNGEAL ENDODERM DERIVED SIGNALING DURING SPECIFICATION OF CRANIOFACIAL SKELETON AND INTERPLAY OF MECHANICAL FORCES AND MOLECULAR SIGNALING DURING SKELETAL CONDENSATION. [Doctoral Dissertation]. Clemson University; 2013. Available from: https://tigerprints.clemson.edu/all_dissertations/1121

Wayne State University

24. Luan, Qing. Pax6 is required for the development of the lateral procephalon in tribolium.

Degree: MS, Biological Sciences, 2012, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_theses/196

► In Tribolium, combinatorial knockdown of the Pax6 orthologs eyeless (ey) and twin of eyeless (toy) affects the peripheral visual system but also other areas… (more)

Subjects/Keywords: Developmental Biology

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APA (6^th Edition):

Luan, Q. (2012). Pax6 is required for the development of the lateral procephalon in tribolium. (Masters Thesis). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_theses/196

Chicago Manual of Style (16^th Edition):

Luan, Qing. “Pax6 is required for the development of the lateral procephalon in tribolium.” 2012. Masters Thesis, Wayne State University. Accessed December 08, 2019. https://digitalcommons.wayne.edu/oa_theses/196.

MLA Handbook (7^th Edition):

Luan, Qing. “Pax6 is required for the development of the lateral procephalon in tribolium.” 2012. Web. 08 Dec 2019.

Vancouver:

Luan Q. Pax6 is required for the development of the lateral procephalon in tribolium. [Internet] [Masters thesis]. Wayne State University; 2012. [cited 2019 Dec 08]. Available from: https://digitalcommons.wayne.edu/oa_theses/196.

Council of Science Editors:

Luan Q. Pax6 is required for the development of the lateral procephalon in tribolium. [Masters Thesis]. Wayne State University; 2012. Available from: https://digitalcommons.wayne.edu/oa_theses/196

Boston University

25. Schatzberg, Daphne. Bioelectrical dynamics are required for normal development of the sea urchin embryo.

Degree: PhD, Biology, 2017, Boston University

URL: http://hdl.handle.net/2144/27332

► Bioelectricity refers to differential membrane voltage and cytoplasmic ion concentrations in tissues or cells which persist over long periods of time. Differences in these steady-state… (more)

▼ Bioelectricity refers to differential membrane voltage and cytoplasmic ion concentrations in tissues or cells which persist over long periods of time. Differences in these steady-state ionic conditions are responsible for large-scale axial patterning and morphogenesis in developing embryos. The sea urchin embryo is an excellent model organism for studying embryonic development, yet a comprehensive study of bioelectricity in sea urchin development has not been reported. Differential ion channel activity is a primary means by which bioelectricity is controlled; thus, we hypothesized that disrupting ion channel activity would reveal the requirements for bioelectricity in the sea urchin embryo. We performed a screen of ion channel inhibitors and discovered that their activities are required for many processes in sea urchin development. We chose two interesting phenotypes to investigate further. First, we demonstrate that H+/K+ ATPase (HKA) activity is required for biomineralization of the sea urchin larval skeleton. We determined that embryos raised with HKA inhibitors initially exhibit voltage and pH changes, then revert to normal voltage and pH during biomineralization via compensatory changes in sodium and chloride ions; it is likely that these compensatory changes lead to defects in transport of carbonate ions, that in turn, inhibit biomineralization of the calcium carbonate skeleton. We hypothesize that similar mechanisms are at play in human patients on long-term HKA inhibitors to treat acid reflux, in whom biomineralization is also decreased. Next, we demonstrate that V-type H+ ATPase (VHA) activity is required for specification of the dorsal-ventral (DV) axis, for the normal inactivation of p38 MAPK in the presumptive dorsal region, and for the subsequent asymmetric onset of expression of the TGFβ family member Nodal, that locally specifies the ventral territory. Embryos treated with VHA inhibitors exhibit global p38 MAPK activity and Nodal expression, and are ventralized. We describe previously unknown gradients of voltage and pH across the DV axis, the sharpness of which requires VHA activity. We propose that the voltage and pH gradients encode spatial information which confers asymmetry on p38 MAPK activity. Overall, we demonstrate that bioelectrical changes are essential for development of the sea urchin embryo, specifically via roles in biomineralization and DV axis specification.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Schatzberg, D. (2017). Bioelectrical dynamics are required for normal development of the sea urchin embryo. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/27332

Chicago Manual of Style (16^th Edition):

Schatzberg, Daphne. “Bioelectrical dynamics are required for normal development of the sea urchin embryo.” 2017. Doctoral Dissertation, Boston University. Accessed December 08, 2019. http://hdl.handle.net/2144/27332.

MLA Handbook (7^th Edition):

Schatzberg, Daphne. “Bioelectrical dynamics are required for normal development of the sea urchin embryo.” 2017. Web. 08 Dec 2019.

Vancouver:

Schatzberg D. Bioelectrical dynamics are required for normal development of the sea urchin embryo. [Internet] [Doctoral dissertation]. Boston University; 2017. [cited 2019 Dec 08]. Available from: http://hdl.handle.net/2144/27332.

Council of Science Editors:

Schatzberg D. Bioelectrical dynamics are required for normal development of the sea urchin embryo. [Doctoral Dissertation]. Boston University; 2017. Available from: http://hdl.handle.net/2144/27332

Wayne State University

26. Li, Lisa. Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity.

Degree: MS, Biological Sciences, 2016, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_theses/495

► In C. elegans, an insulin-like peptide (ILP) network coordinates various physiological processes, including developmental plasticity, to enhance survival under different environments. During stressful conditions,… (more)

Subjects/Keywords: Developmental Biology

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APA (6^th Edition):

Li, L. (2016). Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity. (Masters Thesis). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_theses/495

Chicago Manual of Style (16^th Edition):

Li, Lisa. “Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity.” 2016. Masters Thesis, Wayne State University. Accessed December 08, 2019. https://digitalcommons.wayne.edu/oa_theses/495.

MLA Handbook (7^th Edition):

Li, Lisa. “Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity.” 2016. Web. 08 Dec 2019.

Vancouver:

Li L. Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity. [Internet] [Masters thesis]. Wayne State University; 2016. [cited 2019 Dec 08]. Available from: https://digitalcommons.wayne.edu/oa_theses/495.

Council of Science Editors:

Li L. Regulators Of Ins-6, A Major Node Of The Insulin-Like Peptide Network For Developmental Plasticity. [Masters Thesis]. Wayne State University; 2016. Available from: https://digitalcommons.wayne.edu/oa_theses/495

University of California – Irvine

27. Murad, Rabi. Functional genomic analyses of development in mouse and regeneration in Hydra.

Degree: Biological Sciences, 2018, University of California – Irvine

URL: http://www.escholarship.org/uc/item/7cp748b9

► Gene expression at the transcriptional level is controlled by DNA sequences called cis-regulatory modules (CRM) and at the post-transcriptional level by microRNAs (miRNAs). CRMs have… (more)

▼ Gene expression at the transcriptional level is controlled by DNA sequences called cis-regulatory modules (CRM) and at the post-transcriptional level by microRNAs (miRNAs). CRMs have been studied almost exclusively in bilaterian organisms and little is known about them in non-bilaterian metazoans. Understanding the architecture of CRMs in cnidarians, a sister phylum to bilaterians, can potentially shed light on the evolution of gene regulation. Head regeneration is one of the most widely studied developmental processes in cnidarians. Using a comparison of the transcriptomes of regenerating heads and developing buds, I have determined sets of genes that are specific and common between head regeneration and budding. To understand the genomic sequences controlling these developmental programs, I have mapped the open-chromatin landscape of Hydra in different body parts and during head regeneration to identify candidate promoters and enhancers. My results are the first atlas of CRMs in Hydra, including a substantial fraction that is dynamic during head regeneration. Mammalian embryonic development has been used as a model system to study the role of miRNAs in previous studies, but a complete atlas of miRNA expression during development is missing. To understand the role of microRNAs during mouse development, I analyzed a time course of development representing multiple tissues and organs in mouse embryo. We find distinct tissue and developmental stage-specific miRNA expression profiles dominated by a small number of miRNAs. Analysis of conserved miRNAs reveals clustering of expression patterns by tissue types rather than species. We used matching RNA-seq and histone modification ChIP-seq datasets to improve the annotation of miRNA primary transcripts. We show that the expression levels of majority of primary miRNA transcripts predict the expression of their corresponding mature miRNAs. Our data provide the most comprehensive miRNA resource for mouse as well as a comprehensive list of mouse miRNAs that can be reliably measured by RNA-seq of their primary transcripts.Taken together, the elucidation of cis-regulatory landscape in the cnidarian Hydra and miRNA expression during mouse embryonic development will help the scientific community to understand better the role of enhancers in metazoan evolution and miRNA regulation in mammalian embryonic development.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Murad, R. (2018). Functional genomic analyses of development in mouse and regeneration in Hydra. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/7cp748b9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Murad, Rabi. “Functional genomic analyses of development in mouse and regeneration in Hydra.” 2018. Thesis, University of California – Irvine. Accessed December 08, 2019. http://www.escholarship.org/uc/item/7cp748b9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Murad, Rabi. “Functional genomic analyses of development in mouse and regeneration in Hydra.” 2018. Web. 08 Dec 2019.

Vancouver:

Murad R. Functional genomic analyses of development in mouse and regeneration in Hydra. [Internet] [Thesis]. University of California – Irvine; 2018. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/7cp748b9.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Murad R. Functional genomic analyses of development in mouse and regeneration in Hydra. [Thesis]. University of California – Irvine; 2018. Available from: http://www.escholarship.org/uc/item/7cp748b9

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

28. Monica, Stefanie Dolores. Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio.

Degree: Molecular & Cell Biology, 2016, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/9kx8p8hx

► The neural crest (NC) is a vertebrate-specific population of multipotent cells, often referred to as the fourth germ layer because of its unique ability to… (more)

▼ The neural crest (NC) is a vertebrate-specific population of multipotent cells, often referred to as the fourth germ layer because of its unique ability to migrate to all parts of the body and contribute to a variety of tissues. Positional information allows neural crest cells to determine their location relative to each other and in complex 3D environments, and to respond accordingly. A central question in developmental biology is how these positional cues are imparted to cells, and how the cells interpret them for the appropriate response. The work in this thesis sets out to determine 1) how Wnt and BMP gradients define cells as neural crest, 2) how the position along the AP axis determines neural crest fate and 3) how abolishing one positional cue results in a craniofacial phenotype.Precise control of BMP and Wnt signaling in both space and time is necessary for proper neural crest induction. While the Wnt signaling pathway has been extensively studied, it is not clear how the cells interpret and respond to the Wnt signals. Knowing the importance of these pathways in setting up the embryo axes, I wanted to further our understanding of the precise way these pathways initiate the neural crest program. An important first step toward determining how the intersection of the Wnt and BMP signaling pathways set up the neural crest cell program in space and time was to develop a method to rapidly and uniformly manipulate the signaling pathways in Xenopu laevis embryos. I was able to validate that small molecules can replace the use of mRNA injections in order to manipulate signaling pathways. The next chapter of my thesis determines how neural crest cells are specified at different axial levels. The derivatives and migratory paths of neural crest cells in the cranial and non-cranial regions are very distinct, so the gene regulatory networks are likely to differ as well. Previous work has focused on the cranial neural crest cells, and therefore, regulation of the non-cranial neural crest program remains poorly understood. By taking advantage of transgenic zebrafish lines, I show that a neural crest cell’s position along the AP axis during early development determines its fate. In the final chapter of this thesis, I examine how abolishing one cue (Wnt signaling) affects patterning of neural crest-derived cells without affecting earlier aspects of neural crest formation, such as proliferation and differentiation. Analysis of the craniofacial structures in mutants for multiple components of non-canonical Wnt signaling provides evidence that each component is required for proper formation in different axes.

Subjects/Keywords: Developmental biology

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APA (6^th Edition):

Monica, S. D. (2016). Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/9kx8p8hx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Monica, Stefanie Dolores. “Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio.” 2016. Thesis, University of California – Berkeley. Accessed December 08, 2019. http://www.escholarship.org/uc/item/9kx8p8hx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Monica, Stefanie Dolores. “Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio.” 2016. Web. 08 Dec 2019.

Vancouver:

Monica SD. Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio. [Internet] [Thesis]. University of California – Berkeley; 2016. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/9kx8p8hx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Monica SD. Induction, Specification and Differentiation of Neural Crest Cells in Xenopus and Danio rerio. [Thesis]. University of California – Berkeley; 2016. Available from: http://www.escholarship.org/uc/item/9kx8p8hx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Irvine

29. Zhu, Yilun. Understanding how Pentagone contributes to shaping a Dpp gradient.

Degree: Biological Sciences, 2018, University of California – Irvine

URL: http://www.escholarship.org/uc/item/00n26510

► Although developing organisms of the same species may vary in size, the proportions of body sizes are kept remarkably constant. They do so even in… (more)

Subjects/Keywords: Developmental biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhu, Y. (2018). Understanding how Pentagone contributes to shaping a Dpp gradient. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/00n26510

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhu, Yilun. “Understanding how Pentagone contributes to shaping a Dpp gradient.” 2018. Thesis, University of California – Irvine. Accessed December 08, 2019. http://www.escholarship.org/uc/item/00n26510.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhu, Yilun. “Understanding how Pentagone contributes to shaping a Dpp gradient.” 2018. Web. 08 Dec 2019.

Vancouver:

Zhu Y. Understanding how Pentagone contributes to shaping a Dpp gradient. [Internet] [Thesis]. University of California – Irvine; 2018. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/00n26510.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhu Y. Understanding how Pentagone contributes to shaping a Dpp gradient. [Thesis]. University of California – Irvine; 2018. Available from: http://www.escholarship.org/uc/item/00n26510

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

30. Lee, Samuel. Development of Mouse Brown Adipose Tissue During Perinatal Period.

Degree: Biology, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/7fd860pf

► Although brown adipose tissue (BAT) has recently become a topic of interest due to its newfound presence in adult humans, the morphology brown adipocytes and… (more)

Subjects/Keywords: Developmental biology

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lee, S. (2018). Development of Mouse Brown Adipose Tissue During Perinatal Period. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/7fd860pf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lee, Samuel. “Development of Mouse Brown Adipose Tissue During Perinatal Period.” 2018. Thesis, University of California – San Diego. Accessed December 08, 2019. http://www.escholarship.org/uc/item/7fd860pf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lee, Samuel. “Development of Mouse Brown Adipose Tissue During Perinatal Period.” 2018. Web. 08 Dec 2019.

Vancouver:

Lee S. Development of Mouse Brown Adipose Tissue During Perinatal Period. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2019 Dec 08]. Available from: http://www.escholarship.org/uc/item/7fd860pf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lee S. Development of Mouse Brown Adipose Tissue During Perinatal Period. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/7fd860pf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations