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You searched for subject:(delta tocotrienol). Showing records 1 – 3 of 3 total matches.

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1. Sodhani, Shradha. The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet.

Degree: PhD, Nutrition and Food Sciences, 2012, Texas Woman's University

Nonalcoholic Fatty Liver Disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH), which are histologically similar to alcoholic hepatitis are the most common liver conditions in USA. Diets high in fat have been associated with the development of obesity and NASH. Currently there are no therapeutic drugs approved by FDA available as treatment for these diseases; therefore attention has been directed towards the role of bioactive compounds like polyphenols and fat-soluble vitamin E in the treatment of NAFLD/NASH. The objective of this study was to investigate the effects of dietary grape seed polyphenols (GSP, 1% and 2%) and δ-tocotrienol (δ3T, 0.025% and 0.05%) supplementation alone and in combination, incorporated into a high fat diet (HF) on NASH-like histopathologic features and liver fibrosis in C57BL/6J mice. Mice were placed on HF diets which were supplemented with two doses of GSP, δ-tocotrienol or their combination. Histopathological changes of the liver were assessed using Hematoxylin and Eosin stain, periodic acid Schiff stain (P.A.S) and Masson's trichrome stain and hepatic fibrosis by immunostaining. The morphometric analysis showed that the NASH-like histologic features increased in HF diet fed group, and reduced both by (i) GSP supplementation alone and (ii) in combination with δ3T. Steatosis was reduced by GSP with reduction of the lipid droplet area (p ≤ .01) (cells/mm2). Overall, the HF diet was found to induce steatosis in the mice. The photomicrographs obtained from the three different histology stains showed the beneficial effects of GSP supplementation with reduction in mice mean liver weight, steatosis and absence of collagen accumulation and reduction in the activation of hepatic stellate cells (HSC) and liver fibrosis. Thus dietary GSP supplementation alone and in combination with δ3T in HF diet may ameliorate the NASH-like histopathological features in C57BL/6J mice. Advisors/Committee Members: Imrhan, Victorine (Committee Chair), Mills, Nathaniel (committee member), Vijayagopal, Parakat (committee member), Juma, Shanil (committee member).

Subjects/Keywords: Biological sciences; Health and environmental sciences; Delta tocotrienol; Grape seed polyphenols; High fat diet; NASH histopathology; Nonalcoholic steatohepatitis

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APA (6th Edition):

Sodhani, S. (2012). The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet. (Doctoral Dissertation). Texas Woman's University. Retrieved from https://twu-ir.tdl.org/handle/11274/10573

Chicago Manual of Style (16th Edition):

Sodhani, Shradha. “The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet.” 2012. Doctoral Dissertation, Texas Woman's University. Accessed October 26, 2020. https://twu-ir.tdl.org/handle/11274/10573.

MLA Handbook (7th Edition):

Sodhani, Shradha. “The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet.” 2012. Web. 26 Oct 2020.

Vancouver:

Sodhani S. The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet. [Internet] [Doctoral dissertation]. Texas Woman's University; 2012. [cited 2020 Oct 26]. Available from: https://twu-ir.tdl.org/handle/11274/10573.

Council of Science Editors:

Sodhani S. The effect of grape seed polyphenols and delta tocotrienol as supplements in improvement of NASH histopathology in C57BL/J6 mice fed high fat diet. [Doctoral Dissertation]. Texas Woman's University; 2012. Available from: https://twu-ir.tdl.org/handle/11274/10573

2. Fernandes, Nicolle Valerie. Impact of delta-tocotrienol on human melanoma cell proliferation.

Degree: PhD, Nutrition, 2010, Texas Woman's University

The rate-limiting enzyme of the mevalonate pathway, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, provides essential intermediates for the prenylation or dolichylation of growth-related proteins including nuclear Ras, nuclear lamins, and growth factor receptors. d-δ-tocotrienol, a post-transcriptional down-regulator of HMG CoA reductase, suppresses the proliferation of marine B16 melanoma cells and human blood, breast, cervix, colon, liver, lung, lymph gland, nerve, pancreas, and prostate tumor cells. Dietary d-δ-tocotrienol suppresses the growth of implanted B16 melanomas without toxicity to host mice. We evaluated the impact of d-δ-tocotrienol on the proliferation of human A2058 and A375 melanoma cells. d-δ-tocotrienol induced dose-dependent suppression of the cell proliferation following 72 h incubation in 96-well plates with 50% inhibitory concentrations (IC50) of 37.5 ± 1.4 (A2058) and 22.3 ± 1.8 (A375) μmol/L, respectively. d-δ-tocotrienol-mediated cell cycle arrest at the G1 phase was accompanied by decreased expression of cyclin-dependent kinase 4. Concomitantly, procaspase-3 cleavage and morphological changes detected by fluorescence microscopy following acridine orange and ethidium bromide dual staining showed d-δ-tocotrienol-induced apoptosis in melanoma cells. Consequent to mevalonate deprivation and the putatively reduced prenylation and biological half-life of Ras protein, d-δ-tocotrienol induced concentration- and time dependent decrease in the expression of Ras. The impact of d-8-tocotrienol on A2058 cell proliferation was potentiated by lovastatin (IC50 = 3.1± 0.5 μmol/L), a competitive inhibitor of HMG CoA reductase. d-δ-tocotrienol may have potential application in melanoma chemoprevention and/or therapy. Advisors/Committee Members: Mo, Huanbiao (Committee Chair), DiMarco, Nancy (committee member), Hynds, Diana (committee member), Grossie, Bruce (committee member), Vijayagopal, Parakat (committee member).

Subjects/Keywords: Health and environmental sciences; Biological sciences; Isoprenoids; Melanoma; d-delta-tocotrienol; Molecular biology; Nutrition

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APA (6th Edition):

Fernandes, N. V. (2010). Impact of delta-tocotrienol on human melanoma cell proliferation. (Doctoral Dissertation). Texas Woman's University. Retrieved from http://hdl.handle.net/11274/10679

Chicago Manual of Style (16th Edition):

Fernandes, Nicolle Valerie. “Impact of delta-tocotrienol on human melanoma cell proliferation.” 2010. Doctoral Dissertation, Texas Woman's University. Accessed October 26, 2020. http://hdl.handle.net/11274/10679.

MLA Handbook (7th Edition):

Fernandes, Nicolle Valerie. “Impact of delta-tocotrienol on human melanoma cell proliferation.” 2010. Web. 26 Oct 2020.

Vancouver:

Fernandes NV. Impact of delta-tocotrienol on human melanoma cell proliferation. [Internet] [Doctoral dissertation]. Texas Woman's University; 2010. [cited 2020 Oct 26]. Available from: http://hdl.handle.net/11274/10679.

Council of Science Editors:

Fernandes NV. Impact of delta-tocotrienol on human melanoma cell proliferation. [Doctoral Dissertation]. Texas Woman's University; 2010. Available from: http://hdl.handle.net/11274/10679

3. Hussein, Deema. d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells.

Degree: PhD, Nutrition, 2008, Texas Woman's University

The rate-limiting enzyme of the mevalonate pathway, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, provides essential intermediates for the prenylation or dolichylation of numerous growth-related proteins including nuclear lamins Ras and growth factor receptors. Competitive inhibitors of HMG CoA reductase, the statins, and down-regulators of HMG CoA reductase, the monoterpene geraniol and the sesquitepene farnesol, have been shown to suppress pancreatic tumor growth both in vitro and in vivo by inducing cell cycle arrest and initiating apoptosis. d-δ-Tocotrienol, a vitamin E isomer with a farnesol moiety, has been shown to be growth-suppressive in a number of in vitro and in vivo tumor models. These considerations led to an in vitro evaluation of the tumor-suppressive impact of d-δ-tocotrienol in human MIA PaCa-2 and PANC-1 pancreas carcinoma cells and BxPC-3 pancreas ductal adenocarcinoma cells. d-δ-Tocotrienol induced concentration-dependent suppression of the proliferation of all three cell lines with 50% inhibitory concentrations of 28 ± 6, 35 ± 8, and 35 ± 7 µmol/L for MIA PaCa-2, PANC-1, and BxPC-3, respectively. Cell cycle analyses revealed d-δ-tocotrienol induced Gl arrest in MIA PaCa-2, PANC-1 and BxPC-3 cells. d-δ-Tocotrienol-mediated morphological changes resemble those occurring in apoptotic cells. d-δ-Tocotrienol also induced apoptosis in all three types of pancreatic tumor cells by flow cytometric analysis and fluorescence microscopy. A blend of lovastatin (2 µmol/L) and d-δ-tocotrienol (5 and 10 µmol/L) synergistically suppressed the proliferation of MIA PaCa-2 cells. Supplemental mevalonate attenuated the growth inhibition induced by d-δ-tocotrienol in all three types of pancreatic tumor cells tested. The results of these studies suggested that d-δ-tocotrienol as a down-regulator of the mevalonate pathway may have potential as an adjuvant therapy in pancreatic cancer. Advisors/Committee Members: Mo, Huanbiao (Committee Chair), Grossie, Bruce (committee member), Imrhan, Victorine (committee member), Hsueh, Andie (committee member), Harris, Diane (committee member).

Subjects/Keywords: Health and environmental sciences; Biological sciences; BxPC-3; K-Ras; MIA PaCa-2; PANC-1; Pancreatic cancer; d-delta-tocotrienol

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APA (6th Edition):

Hussein, D. (2008). d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells. (Doctoral Dissertation). Texas Woman's University. Retrieved from http://hdl.handle.net/11274/10845

Chicago Manual of Style (16th Edition):

Hussein, Deema. “d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells.” 2008. Doctoral Dissertation, Texas Woman's University. Accessed October 26, 2020. http://hdl.handle.net/11274/10845.

MLA Handbook (7th Edition):

Hussein, Deema. “d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells.” 2008. Web. 26 Oct 2020.

Vancouver:

Hussein D. d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells. [Internet] [Doctoral dissertation]. Texas Woman's University; 2008. [cited 2020 Oct 26]. Available from: http://hdl.handle.net/11274/10845.

Council of Science Editors:

Hussein D. d-δ-tocotrienol-mediated suppression of the proliferation of human PANC-1, MIA PaCa-2, and BxPC-3 pancreatic tumor cells. [Doctoral Dissertation]. Texas Woman's University; 2008. Available from: http://hdl.handle.net/11274/10845

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