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Coventry University
1.
Janik, I. A.
The detection and prediction of mastitis in dairy cows by particle analysis.
Degree: PhD, 2013, Coventry University
URL: http://curve.coventry.ac.uk/open/items/80b0b311-6986-4dc8-9a62-f6fee636dcdf/1
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629987
► This study investigated the hypothesis that the particulate content of milk, as monitored with particle counters, is correlated to the health status of lactating dairy…
(more)
▼ This study investigated the hypothesis that the particulate content of milk, as monitored with particle counters, is correlated to the health status of lactating dairy cows, in particular the condition mastitis. Twenty Holstein cows were monitored from the very first day of clinical mastitis outbreak until complete recovery from the disease. During the experiment, the changes in particle behaviour in all four quarters and mixture of milk from all of them were measured. For each sample the following parameters were measured: somatic cell count, fat content, lactose and protein concentration, number and size distribution of milk particles, electric conductivity and diameter of milk fat globules. In total over thirty mastitis outbreaks were observed and monitored throughout, including the first phase of this study when over three thousand samples of foremilk were collected and examined. An operational protocol and particle monitoring device were designed with the help of a commercial company Facility Monitoring Systems Ltd (FMS), Malvern. A particle counter and Peak Height Analyser (PHA) were used to monitor particulate content of milk and a compound phase contrast microscope was used to identify milk particles by photographic visualisation and to establish their diameter. It was observed that the number of particles, milk fat globule diameter and somatic cell counts were stable during periods without udder inflammation. Mastitis caused great changes in these parameters. Both milk particulate size and number were significantly affected by clinical and subclinical form of inflammation (change to the particulate behaviour). It was observed that the changes to the volume median diameter (VMD) of fat globules became evident a few days before clinical signs were present. Results obtained from a particle counter and the PHA were in agreement with data obtained by microscopy. Major changes were recorded in the number of total particles in milk before and during the outbreak of mastitis. Further research showed that changes took place in the pattern of particulate behaviour without visible signs of disease; additional data established that subclinical mastitis can be also identified through the monitoring of particles in milk. In summary monitoring of the behaviour (changes to size and number) of milk fag globules (MFG) can be used as an early indicator of the onset of mastitis. In addition data collected during study produced strong evidence supporting the theory of the interdependence of the quarters within the udder. It was found that the coefficient of correlation for size and number of particles for all four quarters within the udder was statistically significant. Particle counts and the VMD values behaviour were similar for the four quarters. This relationship was observed for all monitored animals. Moreover, the same relationship was also observed during both clinical and subclinical outbreaks of mastitis. Somatic cell count was affected only in an infected quarter while particulate content of milk ―responded‖ to disease…
Subjects/Keywords: 636.2; dairy cows, mastitis, monitoring; Dairy farming; Cattle  – Diseases; Lactation; Mastitis
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APA (6th Edition):
Janik, I. A. (2013). The detection and prediction of mastitis in dairy cows by particle analysis. (Doctoral Dissertation). Coventry University. Retrieved from http://curve.coventry.ac.uk/open/items/80b0b311-6986-4dc8-9a62-f6fee636dcdf/1 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629987
Chicago Manual of Style (16th Edition):
Janik, I A. “The detection and prediction of mastitis in dairy cows by particle analysis.” 2013. Doctoral Dissertation, Coventry University. Accessed April 26, 2018.
http://curve.coventry.ac.uk/open/items/80b0b311-6986-4dc8-9a62-f6fee636dcdf/1 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629987.
MLA Handbook (7th Edition):
Janik, I A. “The detection and prediction of mastitis in dairy cows by particle analysis.” 2013. Web. 26 Apr 2018.
Vancouver:
Janik IA. The detection and prediction of mastitis in dairy cows by particle analysis. [Internet] [Doctoral dissertation]. Coventry University; 2013. [cited 2018 Apr 26].
Available from: http://curve.coventry.ac.uk/open/items/80b0b311-6986-4dc8-9a62-f6fee636dcdf/1 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629987.
Council of Science Editors:
Janik IA. The detection and prediction of mastitis in dairy cows by particle analysis. [Doctoral Dissertation]. Coventry University; 2013. Available from: http://curve.coventry.ac.uk/open/items/80b0b311-6986-4dc8-9a62-f6fee636dcdf/1 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.629987

Washington State University
2.
[No author].
Diagnosis of Mycoplasma Mastitis: Validation and Development
.
Degree: 2010, Washington State University
URL: http://hdl.handle.net/2376/2805
► Microbiological culture of milk samples has been used as a standard diagnosis for mycoplasma mastitis. It is suggested to perform with fresh samples for optimum…
(more)
▼ Microbiological culture of milk samples has been used as a standard diagnosis for mycoplasma
mastitis. It is suggested to perform with fresh samples for optimum diagnosis. Submission of fresh samples is often difficult given the logistics of collection and shipping of milk samples from farm to laboratory. Therefore, milk samples are usually chilled or frozen before culture. A study of the effects of storage methods on the recovery of
Advisors/Committee Members: Fox, Lawrence K (advisor).
Subjects/Keywords: dairy cows;
diagnosis;
mastitis;
mycoplasma
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APA (6th Edition):
author], [. (2010). Diagnosis of Mycoplasma Mastitis: Validation and Development
. (Thesis). Washington State University. Retrieved from http://hdl.handle.net/2376/2805
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
author], [No. “Diagnosis of Mycoplasma Mastitis: Validation and Development
.” 2010. Thesis, Washington State University. Accessed April 26, 2018.
http://hdl.handle.net/2376/2805.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
author], [No. “Diagnosis of Mycoplasma Mastitis: Validation and Development
.” 2010. Web. 26 Apr 2018.
Vancouver:
author] [. Diagnosis of Mycoplasma Mastitis: Validation and Development
. [Internet] [Thesis]. Washington State University; 2010. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/2376/2805.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
author] [. Diagnosis of Mycoplasma Mastitis: Validation and Development
. [Thesis]. Washington State University; 2010. Available from: http://hdl.handle.net/2376/2805
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht
3.
Swinkels, J.M.
Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences.
Degree: 2014, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/308798
► Mastitis is an inflammation of the udder caused by bacteria that invade the udder through the teat canal, causing either persistent intramammary infections, or short,…
(more)
▼ Mastitis is an inflammation of the udder caused by bacteria that invade the udder through the teat canal, causing either persistent intramammary infections, or short, transient infections.
Mastitis is the most costly disease on a
dairy farm because it directly affects the production of milk, the primary source of income for the
dairy farmer.
Mastitis can be visible (clinical
mastitis) or invisible (subclinical
mastitis). A large proportion of farmers repeats antibiotic treatment after initial standard treatment according the label claim, thereby extending the duration of treatment. The aim of the thesis is to evaluate the bacteriological, clinical and economic efficacy of extended antibiotic treatment of persistent
mastitis during lactation compared to standard treatment. An additional aim was to explore the social influences on farmers with respect to the duration of antibiotic treatment of clinical
mastitis. The economic outcome of lactational treatment of persistent subclinical Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus
mastitis is highly dependent on the farm, cow and pathogen involved. Extended 8-day lactational treatment is profitable only for very valuable animals, on farms where the risk of pathogen transmission is high and/or on farms where the farmer is likely to cull a high percentage of
cows with subclinical
mastitis. Although clinical efficacy of extended 5-day treatment of clinical Staph. aureus
mastitis was better than a standard 1.5-day cefquinome treatment, bacteriological efficacy was not different. Clinical efficacy of extended 5-day cefquinome treatment, compared to a standard 1.5-day intramammary cefquinome treatment of recurrent clinical
mastitis cases from an environmental origin (E. coli and Strep. uberis), showed an improved clinical outcome compared to standard treatment. Overall bacteriological cure of an extended, 5-day intramammary cefquinome treatment of clinical
mastitis cases of
cows with a persistent cow SCC above 200,000 cells/mL, was not different from a standard, 1.5-day treatment. Extended antibiotic treatment seems to be only indicated when
mastitis cases are caused by streptococci, specifically Strep. uberis. These results indicate that extending treatment of clinical
mastitis with cefquinome should not be routinely recommended. Qualitative interviews to determine social influences on farmers related to the duration of treatment of clinical
mastitis, showed that farmers’ want to treat
mastitis ‘thoroughly’, until clinical symptoms have disappeared. They believe that a ‘good farmer’ treats
mastitis ‘thoroughly’, otherwise it may persist or recur. Extended treatment seems to be part of the social norm of ‘being a good farmer’. Social approval reduces insecurity of potentially being perceived as a poor farmer and thus, extended treatment is emotionally rewarded. Prudent antibiotic use of antibiotics is potentially hindered by perceived subjective norms on duration of treatment. The thesis showed that extending antibiotic treatment of persistent…
Advisors/Committee Members: Lam, Theo.
Subjects/Keywords: Mastitis; dairy; cows; antibiotic; treatment; economics; social
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Swinkels, J. M. (2014). Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/308798
Chicago Manual of Style (16th Edition):
Swinkels, J M. “Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences.” 2014. Doctoral Dissertation, Universiteit Utrecht. Accessed April 26, 2018.
http://dspace.library.uu.nl:8080/handle/1874/308798.
MLA Handbook (7th Edition):
Swinkels, J M. “Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences.” 2014. Web. 26 Apr 2018.
Vancouver:
Swinkels JM. Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2014. [cited 2018 Apr 26].
Available from: http://dspace.library.uu.nl:8080/handle/1874/308798.
Council of Science Editors:
Swinkels JM. Extended antibiotic treatment of persistent bovine mastitis during lactation : Efficacy, economics and social influences. [Doctoral Dissertation]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/308798

Swedish University of Agricultural Sciences
4.
Welderufael, Berihu.
Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows.
Degree: 2017, Swedish University of Agricultural Sciences
URL: https://pub.epsilon.slu.se/14648/
► Mastitis has been the focus of many dairy cattle research projects over the last decades. However, in the genetic evaluation of udder health, only susceptibility…
(more)
▼ Mastitis has been the focus of many dairy cattle research projects over the last decades. However, in the genetic evaluation of udder health, only susceptibility to mastitis has been considered, leaving aside the other aspect of the disease - the recoverability. The aim of this thesis was to improve the genetic evaluation of udder health by introducing a new approach and models that can make use of the information contained in both directions: susceptibility to- and recoverability from mastitis.
In paper I, extensive simulation analyses were performed to develop a bivariate model for joint genetic evaluations of susceptibility to- and recoverability from mastitis. In paper II, the bivariate model with an added time function as well as several systematic effects was applied to real data to estimate genetic parameters in Danish Holstein cows. In paper III, genome-wide association studies were conducted to identify associated single-nucleotide polymorphisms and thereof candidate genes. In paper IV, a dynamic health classification, which takes severity of possible infection into account was introduced to further improve the genetic evaluation of mastitis.
Findings in paper I demonstrated that both traits can be modelled jointly and genetic parameters could be correctly reproduced. In paper II, we detected presence of genetic variation that resulted to heritability (ranging from 0.06 to 0.08) of similar size for both traits. The between trait genetic correlation was -0.83. Despite the strong negative genetic correlation, association signals in paper III did not overlap, suggesting that the traits are at least partially regulated by different genes. Complexity of the traits was manifested with the absence of strong association signals. In paper IV, considerable genetic variation was detected for cows’ presence in health classes defined for longer periods, whereas the variations in health classes defined for short-term and sudden changes (e.g., acute) were mostly attributed to environmental factors. Although susceptibility to- and recoverability from mastitis are strongly negatively correlated, recoverability which is as heritable as susceptibility could be considered a new trait for selection. Evaluating and modelling the ability of animals to overcome infection could be of specific benefit in situations of high disease incidence.
Subjects/Keywords: dairy cows; mastitis; udder health; bivariate model; dairy cow; genetic evaluation; mastitis; recoverability; susceptibility
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Welderufael, B. (2017). Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from https://pub.epsilon.slu.se/14648/
Chicago Manual of Style (16th Edition):
Welderufael, Berihu. “Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows.” 2017. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
https://pub.epsilon.slu.se/14648/.
MLA Handbook (7th Edition):
Welderufael, Berihu. “Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows.” 2017. Web. 26 Apr 2018.
Vancouver:
Welderufael B. Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2017. [cited 2018 Apr 26].
Available from: https://pub.epsilon.slu.se/14648/.
Council of Science Editors:
Welderufael B. Genetic evaluation of susceptibility to- and recoverability from mastitis in dairy cows. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2017. Available from: https://pub.epsilon.slu.se/14648/

University of Nairobi
5.
Ngatia, TA.
Aetiology and pathology of subclinical mastistis in dairy cows
.
Degree: 1988, University of Nairobi
URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/26650
► Three investigations were carried out in this study. Two of them dealt with epidemiological aspects of subclinical mastitis in dairy cows. One of them was…
(more)
▼ Three investigations were carried out in this study. Two of them dealt with epidemiological aspects of subclinical mastitis in dairy cows. One of them was carried out in Danish herds while the other was carried out in Kenyan herds. The third investigation looked into the pathogenesis of bovine mastitis.
Mastitis prevalence rate was measured by examining single quarter milk samples (QMS) for the presence of micro-organisms using cultural methods, and the cellular content utilizing the California Mastitis Test reactions. Other epidemiological aspects of mastitis were investigated by examination of the mammary quarters of each cow and by collecting information concerning mastitis from the owners of these herds.
Mastitis pathogenesis was investigated using ' both gross and microscopic appearances of the teats and the mammary gland parenchyma. The health status of these quarters had previously been assessed by examining six daily milk samples for the presence of micro-organisms and the somatic cell content (SCC), the latter by using automatic electronic cell counting method. In addition, post mortem teat cistern puncture milk samples (TCPS) were examined for the presence of bacteria.
In Denmark, the epidemiological investigation of mastitis involved 5 herds with a total of 122 cows. These cows were of various breeds, and were in different lactation numbers and stages. In all herds, the cows were zero-grazed (confinement-fed), milked by machines, and the owners applied mastitis control and preventive measures.
Microbiological assessment of single quarter milk samples showed that 152 of 478 (31.8%) quarters were infected with one or more types of micro-organisms, and that the 74 of 122 (60.7%) cows were infected in one or more quarters. On the basis of the CMT reaction and taking a score of 3 as mastitis positive, the quarter prevalence rate was 40.5% (184 of 454 quarters). Using the quarter infection status together with CMT score as the diagnostic criteria, 94 of 454 (20.9%) quarters were positive on both tests while 226 of 454 (49.8%)
quarters were mastitis negative. On the other hand,
44 of 454 (9.7%) quarters were bacteriologically positive but CMT negative, while 89 of 454 (19.6%)
quarters were CMT positive but bacteriologically negative.
Six types of micro-organisms were associated with mammary infections in these cows. Staphylococcus aureus was the most commonly encountered organism accounting for 66.7% (106 of 159) isolations. The
others were isolated as follows: Streptococcus spp.,
21.4% (34 of 159); micrococci, 6.9% (11 of 159); Coliforms, 2.5% (4 of 159); Corynebacterium pyogenes, 1.9% (3 of 159), and yeasts, 0.6% (1 of 159) isolations.
The 68 Danish dairy cows (in one herd) involved in the study of mastitis pathogenesis were found to be of different breeds and ages. They were also in different
phases of the lactation period. The owner of this herd used a machine for milking and practised mastitis control and preventive measures.
Of the 272 quarters, 9 (3.3%) of them were dry while only 263 yielded…
Subjects/Keywords: Mastitis;
Dairy Cows;
Pathology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ngatia, T. (1988). Aetiology and pathology of subclinical mastistis in dairy cows
. (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/26650
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ngatia, TA. “Aetiology and pathology of subclinical mastistis in dairy cows
.” 1988. Thesis, University of Nairobi. Accessed April 26, 2018.
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/26650.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ngatia, TA. “Aetiology and pathology of subclinical mastistis in dairy cows
.” 1988. Web. 26 Apr 2018.
Vancouver:
Ngatia T. Aetiology and pathology of subclinical mastistis in dairy cows
. [Internet] [Thesis]. University of Nairobi; 1988. [cited 2018 Apr 26].
Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/26650.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ngatia T. Aetiology and pathology of subclinical mastistis in dairy cows
. [Thesis]. University of Nairobi; 1988. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/26650
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University
6.
Zimov, Jennifer Laura.
Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis.
Degree: MS, Animal Sciences, 2009, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1253132975
► The behavioral and physiological effects of lipopolysaccharide induced mastitis were examined in lactating Holstein cows. Twenty cows were assigned to five blocks of four cows…
(more)
▼ The behavioral and physiological effects of
lipopolysaccharide induced
mastitis were examined in lactating
Holstein
cows. Twenty
cows were assigned to five blocks of four
cows grouped by parity and stage of lactation. The experimental
design for this study was a 2 X 2 factorial arrangement.
Cows
within the blocks were randomly assigned to one of four treatments:
1) intramammary infusion with 25 µg lipopolysaccharide and
intravenous flunixin meglumine; 2) intramammary infusion with 25 µg
lipopolysaccharide and intravenous PBS; 3) intramammary infusion
with PBS and intravenous flunixin meglumine; and 4) intramammary
infusion PBS and intravenous with PBS. Uninfected mammary quarters
were infused 3 h post milking. Intravenous treatments were given 7
h post milking. Experimental
cows were under continuous video
monitoring during the study.
Cows receiving the lipopolysaccharide
treatment had higher mean peak quarter milk somatic cell counts,
rectal temperatures, concentrations of milk amyloid and serum
cortisol during the first 12 h after infusion compared with
intramammary saline treated
cows. Lipopolysaccharide treated
cows
spent a reduced percentage of the first 24 h after challenge eating
and cud chewing compared with saline infused
cows. Rumen
contractions were reduced in lipopolysaccharide infused
cows
compared with saline infused
cows at sample times corresponding
with peak rectal temperatures. Flunixin treatment 4 h after
intramammary challenge mitigated the clinical systemic responses of
increased rectal temperature and decreased rumen activity.
Cows
receiving LPS infusion and treatment with flunixin spent more time
eating the first 12 h after challenge than animals receiving
infusion of LPS and treatment of PBS. Flunixin treatment increased
time spent cud chewing in all
cows. Acute clinical
mastitis changed
physiological and behavioral parameters in lactating
dairy cows.
The administration of flunixin meglumine mitigated the adverse
systemic affects associated with LPS induced
mastitis.
Advisors/Committee Members: Hogan, Joseph (Advisor).
Subjects/Keywords: Animal Diseases; Animals; Welfare; mastitis; dairy cows, bovine, pain, welfare, behavior
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zimov, J. L. (2009). Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1253132975
Chicago Manual of Style (16th Edition):
Zimov, Jennifer Laura. “Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis.” 2009. Masters Thesis, The Ohio State University. Accessed April 26, 2018.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1253132975.
MLA Handbook (7th Edition):
Zimov, Jennifer Laura. “Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis.” 2009. Web. 26 Apr 2018.
Vancouver:
Zimov JL. Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis. [Internet] [Masters thesis]. The Ohio State University; 2009. [cited 2018 Apr 26].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1253132975.
Council of Science Editors:
Zimov JL. Behavioral and Physiological Responses To Lipopolysaccharide
Induced Clinical Mastitis. [Masters Thesis]. The Ohio State University; 2009. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1253132975

Swedish University of Agricultural Sciences
7.
Capurro, Aldo.
Diagnostic and epidemiological studies of staphylococci in bovine mastitis.
Degree: 2009, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1942/
► Diagnostic and epidemiological studies of staphylococci in bovine mastitis.Mastitis is the most common disease in dairy cows and is often caused by staphylococcal infections. The…
(more)
▼ Diagnostic and epidemiological studies of staphylococci in bovine mastitis.Mastitis is the most common disease in dairy cows and is often caused by staphylococcal infections. The genus Staphylococcus is divided in coagulase-negative (CNS) and coagulase-positive (CPS) staphylococci. The CPS S. aureus is the most prevalent udder pathogen in Swedish dairy cows. For successful mastitis control accurate diagnostics and good understanding of the bacterial epidemiology is essential. This thesis describes methods for species differentiation within CNS and CPS, examines genotypic diversity of S. aureus isolates within Sweden, and identifies potential sources of S. aureus in herds with mastitis problems. First, three phenotypic tests (P-agar with acriflavin, β-galactosidase, and haemolytic reaction in chocolate agar) of eight biochemical tests evaluated were found useful for differentiation between the CPS species, S. aureus, S. hyicus and S. intermedius. The proportions of each species among bovine milk isolates were 97%, 1%, and 2%, respectively. Then, species identification of CNS using the Staph-Zymâ„¢ test was compared with sequencing of part of the tuf gene. Staph-Zymâ„¢ correctly identified 61%, but gave an incorrect species name in 28% of the milk isolates. Supplementary tests were frequently needed when using Staph-Zymâ„¢. In the next study, S. aureus isolates from a national survey on acute clinical mastitis were genotyped using pulsed-field gel electrophoresis (PFGE), and 25 pulsotypes (PTs) were identified. Three of the PTss accounted for over 50% of the isolates and were found all over the country. The distribution of PTs was different in the southern region than in the northern and middle regions of the country. Finally, S. aureus PTs in quarter milk samples, body samples (BS), and environment samples (ES) from various animal groups were studied in five herds with S. aureus mastitis problems. Herd differences were found, but all herds had one predominant unique milk PTs. In three farms this PTs was often found in BS and ES of lactating cows, and occasionally in samples from the other groups.
Subjects/Keywords: dairy cows; bovine mastitis; staphylococcus aureus; phenotypes; genotypes; diagnosis; epidemiology; Bovine mastitis; dairy cows; clinical mastitis; Staphylococcus aureus; CNS; CPS; phenotypic; genotypic; Staph-ZymTM; tuf gene sequencing; PFGE
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Capurro, A. (2009). Diagnostic and epidemiological studies of staphylococci in bovine mastitis. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1942/
Chicago Manual of Style (16th Edition):
Capurro, Aldo. “Diagnostic and epidemiological studies of staphylococci in bovine mastitis.” 2009. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/1942/.
MLA Handbook (7th Edition):
Capurro, Aldo. “Diagnostic and epidemiological studies of staphylococci in bovine mastitis.” 2009. Web. 26 Apr 2018.
Vancouver:
Capurro A. Diagnostic and epidemiological studies of staphylococci in bovine mastitis. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2009. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/1942/.
Council of Science Editors:
Capurro A. Diagnostic and epidemiological studies of staphylococci in bovine mastitis. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2009. Available from: http://pub.epsilon.slu.se/1942/
8.
Barbosa Lima, Maria Gabriela.
Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia.
Degree: Mestrado, ClÃnica Veterinária, 2014, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-19092014-090029/
;
► O presente estudo objetivou-se avaliar o perfil das citocinas como indicador prognóstico em diferentes protocolos de tratamento da mamite bovina. De um total de 130…
(more)
▼ O presente estudo objetivou-se avaliar o perfil das citocinas como indicador prognóstico em diferentes protocolos de tratamento da mamite bovina. De um total de 130 vacas em lactação da raça holandesa foram selecionadas cinco vacas sem alteração ao exame do leite (C-), cinco vacas sem grumos no leite, mas com CMT positivo e 30 vacas com mamite clÃnica. Os animais com mamite clÃnica foram alocados em três grupos. O grupo G1 (n=10) recebeu tratamento com antibiótico sistêmico (enrofloxacina), enquanto o grupo G2 (n=10) recebeu somente antibiótico intramamário (sulfato de cefquinoma) e o grupo G3 (n=10) foi tratado com os dois princÃpios (enrofloxacina sistêmico e sulfato de cefquinoma intramamário). Os grupos controle foram formados respectivamente por animais negativos para as duas provas (C1; n=5), e negativos para a prova de fundo escuro e positivos para CMT (C2; n=5). Amostras de leite e sangue foram colhidas antes do tratamento (M0) e no segundo (M1), quinto (M2) e décimo segundo dia (M3) após o término do tratamento para serem submetidas a exame bacteriológico do leite, antibiograma do leite, dosagem de citocinas no leite e no sangue (IL-1, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α INF-γ) e dosagem de imunoglobulinas no leite e no sangue (IgG1, IgG2, IgM e IgA). Foi verificada a normalidade da distribuição dos resultados, utilizando-se teste de Anderson-Darling. Para a avaliação das diferenças entre as médias dos resultados obtidos, foram realizados os testes de análise de variância ANOVA One-way (Unstacked) (para dados com distribuição normal) e Mann-Whitney (para dados que não apresentaram distribuição normal), sendo as análises consideradas significativas as que apresentaram P≤0,05. Todos os animais do G3 apresentaram cura clÃnica e 80% cura bacteriológica nos momentos subsequentes ao tratamento (M1a M3). Ao se avaliar a resposta imunológica desse grupo, notou-se primeiramente o aumento de IL-6 sérico no M1 acompanhado de IgG1 e IgG2, os quais juntamente com o aumento de IL-4 e TNF-α perpetuaram a inflamação até o M2, enquanto que na glândula mamária já se iniciava a resolução da inflamação com o aumento de IL-10. Por outro lado, os animais do grupo G2 apresentaram cura clÃnica e bacteriológica no M1, porém esta não se manteve. Imunologicamente, após o tratamento, o perfil predominante desse grupo foi o Th2 com aumento de IgG1 e IL-4. Ao final do experimento, alguns animais do grupo G2 voltaram a manifestar a doença clinicamente, e tal fato foi acompanhado da diminuição das concentrações de IL-4, IgG1 e de IL-6 no leite. Nos animais do grupo G1 houve cura clÃnica não absoluta e 40% de cura bacteriológica no M3. Imunologicamente, observou-se altos nÃveis de IL-6 e IL-8 no leite durante todo o perÃodo de tratamento e diminuição de IgG2 sérico no M3 correlacionado negativamente com a IgG2 láctea, caracterizando uma resposta local tardia. Os resultados permitem inferir que o acompanhamento por mais de duas semanas da dinâmica da resposta imunológica depende da eficiência do protocolo antimicrobiana…
Advisors/Committee Members: Libera, Alice Maria Melville Paiva Della.
Subjects/Keywords: Dairy cows; Immune Response; Mamite; Mastitis; Prognosis; Prognóstico; Resposta imune; Tratamento; Treatment; Vacas leiteiras
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APA (6th Edition):
Barbosa Lima, M. G. (2014). Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10136/tde-19092014-090029/ ;
Chicago Manual of Style (16th Edition):
Barbosa Lima, Maria Gabriela. “Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia.” 2014. Masters Thesis, University of São Paulo. Accessed April 26, 2018.
http://www.teses.usp.br/teses/disponiveis/10/10136/tde-19092014-090029/ ;.
MLA Handbook (7th Edition):
Barbosa Lima, Maria Gabriela. “Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia.” 2014. Web. 26 Apr 2018.
Vancouver:
Barbosa Lima MG. Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2018 Apr 26].
Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-19092014-090029/ ;.
Council of Science Editors:
Barbosa Lima MG. Perfil das citocinas no prognóstico da mamite bovina após antibioticoterapia. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/10/10136/tde-19092014-090029/ ;

INP Toulouse
9.
Albaaj, Ahmad.
Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows.
Degree: Docteur es, Pathologie, Toxicologie, Génétique et Nutrition, 2017, INP Toulouse
URL: http://www.theses.fr/2017INPT0054
► La fertilité des vaches laitières s’est beaucoup dégradée au cours des dernières décennies, tant à l’échelle nationale qu’internationale. L’objectif poursuivi est d’apporter un éclairage épidémiologique…
(more)
▼ La fertilité des vaches laitières s’est beaucoup dégradée au cours des dernières décennies, tant à l’échelle nationale qu’internationale. L’objectif poursuivi est d’apporter un éclairage épidémiologique sur les relations dynamiques entre la conception (réussite à l’insémination artificielle ; IA), les mammites subcliniques et les déséquilibres métabolico-nutritionnels que sont la cétose subclinique et les déséquilibres azotés de la ration. Un premier chapitre bibliographique dresse l’état de l’art des liens entre performances de reproduction et (i) mammites, (ii) cétose subclinique et (iii) excès de protéines dégradables, en identifiant clairement les interactions et associations croisées entre ces différents composants, et en quantifiant autant que possible ces liens. Les trois parties suivantes mobilisent des données exhaustives du contrôle laitier français sur la période 2008-2012, qui ont été confrontées aux données d’IA. La variable d’intérêt retenue est la conception après IA, soit pour la première IA (IA1) ou pour toutes les IA. Les variations des concentrations de cellules somatiques (CCS) autour de l’IA ont été utilisées pour décrire la dynamique des mammites subcliniques autour de l’IA, selon 4 classes (Bas-Bas, Bas-Haut, Haut-Bas et Haut-Haut) et pour différents seuils de CCS. Le statut de cétose subclinique a été évalué grâce aux taux butyreux et protéique du lait. Les concentrations d’urée du lait sont utilisées pour caractériser les déséquilibres azotés. Le second chapitre propose une description succincte des résultats de production et de reproduction des troupeaux bovins laitiers français sur la période d’étude. Le troisième chapitre focalise sur l’interaction entre les CCS et la cétose subclinique dans un modèle de régression de Poisson expliquant la conception. Les chances de conception à l’IA1 sont réduites de 14% (Risque relatif = 0,86 [IC 95%=0,85–0,87]) pour les groupes Bas-Haut et Haut-Haut, comparé au groupe Bas-Bas, et de 3 à 17 %, selon les définitions retenues, lors de cétose subclinique comparé à son absence. Les résultats identifient et quantifient clairement l’interaction entre la cétose subclinique et les mammites subcliniques dans leur association avec la conception : la baisse de la conception est jusqu’à 2 fois supérieure lors de la présence simultanée d’une augmentation des CCS et d’une cétose subclinique par rapport à la situation où il y a seulement augmentation des CCS. Le quatrième chapitre, mobilisant des méthodes proches de celles du chapitre précédent, montre que la baisse de la concentration d’urée dans le lait autour de l’IA (en dessous de 150 mg/kg, 2,6 mmol/L) est associée à une baisse de la conception de 5 à 9% (Risque relatif = 0.91 (IC95%=0,87-0,96)]) par rapport à des concentrations d’urée du lait qui restent stables (250 - 450 mg/kg ou 4,3-7,7 mmol/L). Ceci révèle l’importance de la stabilité du métabolisme azoté autour de la conception, y compris pour des variations d’urée du lait ou du sang à la baisse, alors que seule la hausse de la concentration d’urée était…
Advisors/Committee Members: Foucras, Gilles (thesis director), Raboisson, Didier (thesis director).
Subjects/Keywords: Bovins laitiers; Fertilité; Mammite; Cétose subclinique; Urée; Dairy cows; Reproduction; Mastitis; Ketosis; Urea
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Albaaj, A. (2017). Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows. (Doctoral Dissertation). INP Toulouse. Retrieved from http://www.theses.fr/2017INPT0054
Chicago Manual of Style (16th Edition):
Albaaj, Ahmad. “Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows.” 2017. Doctoral Dissertation, INP Toulouse. Accessed April 26, 2018.
http://www.theses.fr/2017INPT0054.
MLA Handbook (7th Edition):
Albaaj, Ahmad. “Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows.” 2017. Web. 26 Apr 2018.
Vancouver:
Albaaj A. Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows. [Internet] [Doctoral dissertation]. INP Toulouse; 2017. [cited 2018 Apr 26].
Available from: http://www.theses.fr/2017INPT0054.
Council of Science Editors:
Albaaj A. Dynamiques individuelles et interactions entre santé mammaire, déséquilibres nutritionnels et l'établissement de la gestation chez la vache laitière : Individual dynamics and interactions of udder health, nutritional disorders and conception establishment in dairy cows. [Doctoral Dissertation]. INP Toulouse; 2017. Available from: http://www.theses.fr/2017INPT0054

Swedish University of Agricultural Sciences
10.
Nielsen, Christel.
Economic impact of mastitis in dairy cows.
Degree: 2009, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1968/
► This thesis aims to assess the economic loss associated with clinical (CM) and subclinical (SCM) mastitis under current Swedish farming conditions. Stochastic simulation was used…
(more)
▼ This thesis aims to assess the economic loss associated with clinical (CM) and subclinical (SCM) mastitis under current Swedish farming conditions. Stochastic simulation was used to investigate the impact of mastitis on technical and economic results of a 150-cow dairy herd. The yearly avoidable cost of mastitis, assuming that the initial incidence (32 and 33 cases of CM and SCM per 100 cow-years, respectively) could be reduced by 50%, was estimated at €8 095. This figure corresponded to 5% of the economic net return for the herd given the initial incidence of mastitis. Expressed as an average per cow/year, the avoidable cost of mastitis was estimated at €54. The economic loss associated with mastitis could not be reduced by discarding milk with high somatic cell count, because this resulted in a substantial decrease of the volume of sold milk which was not offset by the increase in milk price. Cases of CM and SCM were on average associated with an average economic loss of €275 and €60, respectively. Reduced milk production constituted the major cost component of the economic loss caused by mastitis. The magnitude of yield loss associated with mastitis occurring in different stages of lactation was assessed using mixed linear models. The dataset was collected in a research herd between 1987 and 2004, and consisted of weekly test-day records sampled in 1200 lactations. The most extensive yield loss was estimated when CM developed in early lactation and when SCM (modelled by means of increased somatic cell count) occurred in late lactation. The 305-day yield loss associated with CM varied between 0 and 705 kg milk in primiparous cows and between 0 and 902 kg milk in multiparous cows, depending on lactation week at onset. Most cases of CM developed in the first week of lactation and resulted in a yield loss of 578 and 782 kg milk in primiparous and multiparous cows, respectively. Daily yield loss at an SCC of 500 000 cells/ml ranged from 0.7 to 2.0 kg milk in primiparous cows and from 1.1 to 3.7 kg milk in multiparous cows. The yield loss in an average 305-day lactation affected by SCM was 150 and 450 kg milk in primiparous and multiparous cows, respectively.
Subjects/Keywords: dairy cows; mastitis; somatic cell count; disease control; milk yield; losses; costs; sweden; dairy cow; mastitis; somatic cell count; yield loss; dairy herd; economic performance; discarding milk
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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APA (6th Edition):
Nielsen, C. (2009). Economic impact of mastitis in dairy cows. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1968/
Chicago Manual of Style (16th Edition):
Nielsen, Christel. “Economic impact of mastitis in dairy cows.” 2009. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/1968/.
MLA Handbook (7th Edition):
Nielsen, Christel. “Economic impact of mastitis in dairy cows.” 2009. Web. 26 Apr 2018.
Vancouver:
Nielsen C. Economic impact of mastitis in dairy cows. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2009. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/1968/.
Council of Science Editors:
Nielsen C. Economic impact of mastitis in dairy cows. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2009. Available from: http://pub.epsilon.slu.se/1968/

University of Edinburgh
11.
Chiumia, Daniel.
Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus.
Degree: 2011, University of Edinburgh
URL: http://hdl.handle.net/1842/5961
► The general aims of the work were to determine the factors associated with vulnerability in Holstein- Friesian dairy cows in two scenarios: A) culling and…
(more)
▼ The general aims of the work were to determine the factors associated with vulnerability in Holstein-
Friesian
dairy cows in two scenarios: A) culling and B) the effect of temperament on days to first
recorded oestrus in
dairy cows. Vulnerability was defined as either an increased risk of being culled
or having long interval from calving to first recorded oestrus in
cows. The work was carried out in
two distinct studies. The objective of the first study was to identify the predisposing factors for an
increased risk of culling in adult Holstein-Friesian
dairy cows. This study was conducted using data
sourced from Scottish Agricultural College Langhill database. Between September 2003 and August
2010, 519
cows calved for the first time and 175 of these were culled. The major reasons for culling
were fertility (9.2%), udder problems (9.1%) and accident (6.2%) on which further analysis was
performed. The culled
cows were matched with their cohorts that survived to a later lactation.
Cows
assessed for the risk of being culled due to major reasons had a mean age at first calving of 26.2
months (Standard Deviation (SD) =2.4).
Cows with high body condition score (BCS) at service and
low 60-day (60d) milk protein had a significantly (P<0.05) increased likelihood of being culled due to
infertility. The regression estimate (RE) was 1.67 for Service BCS and -2.43 for 60d milk protein with
predicted probability (PP) of 0.91. However in first lactation heifers, only BCS at service was
significant (P<0.05, RE=2.65 and PP=0.86).
Cows with a reduced interval to reach peak milk yield
had a significant (P<0.01) likelihood of being culled due to udder problems (RE=-0.05 and PP=
0.89). Locomotion score and parity were not significant on increasing the risk of culling
cows due to
accident. Hence higher BCS at service, low 60d milk protein and short duration to peak lactation are
factors that place
dairy cows at an increased risk of being culled. The second study aimed at
determining the association between temperament and days to first recorded oestrus in adult
dairy
cows. Temperament traits that were studied were flight response score, nervous, interest, shy, bold,
fear and docile. Number of days to first recorded oestrus after calving was the dependent variable in
the analysis. Seventy Holstein-Friesian
dairy cows, mean age at first calving 25 months (SD=1.9)
were used in the study. Temperament traits for individual
cows were recorded on day 30 before
calving, and on days 30 and 60 after calving. Temperament traits were quantified using an Approach
Passageway test. On average the first oestrus occurred 55.5 days (SD=17.9) after calving. The study
showed that temperament traits did not significantly affect the number of days to first recorded
oestrus. Within the temperament traits, nervousness, shyness, boldness, fearfulness and docility were
significantly (P<0.001) related to flight response score while interest was not. In conclusion, higher
than average BCS at service, low milk protein content at day 60 in lactation and short duration…
Advisors/Committee Members: Macrae, Alastair.
Subjects/Keywords: dairy cows; cows; culling; fertility
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chiumia, D. (2011). Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus. (Thesis). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5961
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chiumia, Daniel. “Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus.” 2011. Thesis, University of Edinburgh. Accessed April 26, 2018.
http://hdl.handle.net/1842/5961.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chiumia, Daniel. “Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus.” 2011. Web. 26 Apr 2018.
Vancouver:
Chiumia D. Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus. [Internet] [Thesis]. University of Edinburgh; 2011. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/1842/5961.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chiumia D. Vulnerability in Holstein-Friesian dairy cows : risk factors for culling and effect of temperament on oestrus. [Thesis]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5961
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University
12.
Newman, Kari A.
Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off.
Degree: MS, Veterinary Preventive Medicine, 2008, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1221510039
► During the dry period of the dairy cow there is a rapid increase in natural protective factors. This occurs once the volume of milk…
(more)
▼ During the dry period of the
dairy cow there
is a rapid increase in natural protective factors. This occurs once
the volume of milk accumulated in the udder begins to decrease.
Cows are most susceptible to new intramammary infections (IMI)
during the early dry period and in the weeks immediately prior to
parturition. An intermittent milking schedule at
the end of lactation reduces milk yield prior to drying off. A
reduced milk volume quickens time to teat closure. Longer teat
closure times are associated with a greater prevalence of IMI at
calving. Our objective was to determine whether an intermittent
milking schedule increased lactoferrin concentrations prior to
drying off, and whether reducing the milk yield was associated with
the prevalence of IMI at calving. Results indicated that an
intermittent milking schedule successfully increased lactoferrin
concentrations. A reduced milk yield at drying off was not
associated with infection status at
calving.
Advisors/Committee Members: Rajala-Schultz, Paivi (Advisor).
Subjects/Keywords: Agriculture; Animal Diseases; Animals; Veterinary Services; dairy cows; intermittent milking; lactoferrin; mastitis; infection status; drying off
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Newman, K. A. (2008). Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1221510039
Chicago Manual of Style (16th Edition):
Newman, Kari A. “Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off.” 2008. Masters Thesis, The Ohio State University. Accessed April 26, 2018.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1221510039.
MLA Handbook (7th Edition):
Newman, Kari A. “Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off.” 2008. Web. 26 Apr 2018.
Vancouver:
Newman KA. Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off. [Internet] [Masters thesis]. The Ohio State University; 2008. [cited 2018 Apr 26].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1221510039.
Council of Science Editors:
Newman KA. Factors Affecting Lactoferrin Concentration at Drying Off
and Infection Status at Calving in Dairy Cows on an Intermittent
Milking Schedule Prior to Drying Off. [Masters Thesis]. The Ohio State University; 2008. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1221510039

Swedish University of Agricultural Sciences
13.
Lam, Vo.
Milk production on smallholder dairy cattle farms in Southern Vietnam.
Degree: 2011, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/8052/
► Dairy production is a rather new and not a traditional system in Vietnam. It is mainly based on smallholder dairy farms. The general aim of…
(more)
▼ Dairy production is a rather new and not a traditional system in Vietnam. It is mainly based on smallholder dairy farms. The general aim of the studies in this thesis was to improve milk production on smallholder dairy farms in Southern Vietnam and also to create a foundation that could be used in the advisory service or/and in further research for better milking management routines. Studies were done to cover the specific objectives of this thesis. The studies were designed to identify the problems for dairy production on smallholder dairy farms, to investigate which are the management factors that influenced milk somatic cell count (SCC) in lactating cows, identify the prevalence of subclinical mastitis based on SCC and to study the protein degradation caused by Streptococcus (Str.) agalactiae.
The survey study indicated that the majority of the farmers kept between 2 to 17 cows (mean = 12). The main breed of dairy cow was Holstein Friesian (HF) crosses. This HF cows produced about 16 kg/day/cow. Around 35% of the farms provided fresh water ad libitum for the cows, while 51 % provided less than 30 L of water per cow per day. Moreover, milk SCC was high (1,300,000 cells/mL milk) in many of the studied farms. The second study found that limited to drinking water significantly increased herd SCC. Str. agalactiae was found to be a predominant species in infected udders. Further investigation showed that the prevalence of subclinical mastitis (SCC > 200,000 cells/mL milk) at quarter basis was 63.2% (285 out of 451) and at cow basis 88.6% (101 out 114). Str. agalactiae was found on 65% farms, 35.6% cows (41 out of 115) and 21% quarters (96 out of 458). Among 96 isolates of Str. agalactiae, 11 different strains were identified. The proteolysis of casein was higher (12-70%) compared with whey proteins (4-12%). The strains of Str. agalactiae in the same phylogenic group did not show the same degradation of casein and whey protein. Str. aglactiae caused proteolytic activity where the proteolysis of αS2-casein was highest, up to 70%, compared with control milk. Proteolytic activity caused by different strains showed a large variation. The lowest breakdown of casein was found to be 30% compared with control milk.
Overall, the high milk SCC in this present study showed poor udder health of lactating cows on smallholder farms. The high milk SCC was mainly caused by the infection of udders with Str. agalactiae.
Subjects/Keywords: dairy cows; mastitis; somatic cell count; udder health; agalactia; streptococcus agalactiae; proteolysis; dairy farms; smallholders; viet nam; smallholder dairy farm; somatic cell count; management factors; udder health; Streptococcus agalactiae
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lam, V. (2011). Milk production on smallholder dairy cattle farms in Southern Vietnam. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/8052/
Chicago Manual of Style (16th Edition):
Lam, Vo. “Milk production on smallholder dairy cattle farms in Southern Vietnam.” 2011. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/8052/.
MLA Handbook (7th Edition):
Lam, Vo. “Milk production on smallholder dairy cattle farms in Southern Vietnam.” 2011. Web. 26 Apr 2018.
Vancouver:
Lam V. Milk production on smallholder dairy cattle farms in Southern Vietnam. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2011. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/8052/.
Council of Science Editors:
Lam V. Milk production on smallholder dairy cattle farms in Southern Vietnam. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2011. Available from: http://pub.epsilon.slu.se/8052/

Swedish University of Agricultural Sciences
14.
Lundberg, Ã…sa.
Mastitis in dairy cows.
Degree: 2015, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/11975/
► Mastitis, inflammation of the udder, is a common disease among dairy cows worldwide. This thesis investigated the genotype variation and spread of three major udder…
(more)
▼ Mastitis, inflammation of the udder, is a common disease among dairy cows worldwide. This thesis investigated the genotype variation and spread of three major udder pathogens: Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis. Isolates collected in a previous study of veterinary-treated clinical mastitis (VTCM) were used to study between-herd genotype variation in epidemiologically independent isolates and differences in outcome. Intramammary infections (IMI) were scrutinized for their occurrence on the day of calving and four days later by quarter milk sampling in selected herds with mastitis problems. The importance for long-term udder health and production of these IMI was also investigated.
The two most common Staph. aureus genotypes among the VTCM isolates were detected in 64% of the herds. In contrast, none of almost 100 Strep. uberis isolates from different herds was of the same genotype. The Strep. dysgalactiae isolates varied moderately compared to the ones of Staph. aureus and Strep. uberis. The common genotypes of Staph. aureus were associated with a lower somatic cell count (SCC) during the follow-up period, compared to the less common genotypes. No differences were detected between genotypes of streptococci, but cows with Strep. dysgalactiae VTCM had a lower SCC during the follow-up period compared to those with Strep. uberis. In herds with mastitis problems, Staph. aureus was the most common pathogen found at and just after calving, followed by Strep. dysgalactiae, and Strep. uberis. Isolates of Staph. aureus showed the lowest within-herd genotype diversity, followed by an intermediate diversity of Strep. dysgalactiae and a high diversity of Strep. uberis. There was a marked variation in occurrence of IMI at or close to calving in herds with mastitis problems, indicating that the predisposing factors for udder infections at calving differed between herds. Most early lactation IMI were associated with an increase in lactation SCC, whereas associations with other outcome variables were more variable. Altogether, this thesis contributes knowledge about Staph. aureus, Strep. dysgalactiae, and Strep. uberis that can be used in preventive work against these IMI.
Subjects/Keywords: dairy cows; bovine mastitis; staphylococcus aureus; streptococcus; pathogens; genotypes; genetic variation; mammary glands; lactation; Staphylococcus aureus; Streptococcus dysgalactiae; Streptococcus uberis; clinical mastitis; intramammary infection; outcome; bacterial genotype; early lactation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Lundberg, Ã. (2015). Mastitis in dairy cows. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/11975/
Chicago Manual of Style (16th Edition):
Lundberg, Ã…sa. “Mastitis in dairy cows.” 2015. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/11975/.
MLA Handbook (7th Edition):
Lundberg, Ã…sa. “Mastitis in dairy cows.” 2015. Web. 26 Apr 2018.
Vancouver:
Lundberg Ã. Mastitis in dairy cows. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2015. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/11975/.
Council of Science Editors:
Lundberg Ã. Mastitis in dairy cows. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2015. Available from: http://pub.epsilon.slu.se/11975/

Addis Ababa University
15.
Yien, Deng.
ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
.
Degree: 2014, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/5547
► A cross sectional study was conducted in Jikawo Woreda of Nuer Zone, Gambella Regional State in Southwest Ethiopia, with the objective of assessing hygienic milk…
(more)
▼ A cross sectional study was conducted in Jikawo Woreda of Nuer Zone, Gambella Regional State in Southwest Ethiopia, with the objective of assessing hygienic milk production practices, prevalence of
mastitis and associated risk factors from December 2013 to April 2014. Data were collected through administering semi-structured questionnaire, group discussion and conducting California
Mastitis screening test across the randomly selected households of the three purposively selected kebeles in the Woreda. The questionnaire was administered to 145 households. The results showed that 75.2% (109) of interviewed households were females while the remaining were males who were involved in hygienic milk production responses. Washing udder was not practiced by 95.17% (138) of the households in the area. Only 4.83% (7) practiced washing udder before milking only after the death of the calf. About 48.3% (70) of the households use cold water to clean milk handling equipments. The California
Mastitis Screening Test was performed on 121 lactating
dairy cows of these; about 60.33% (73) were showed
mastitis cases. The prevalence of clinical and subclinical
mastitis was found to be 11.57% (14) and 48.76% (59), respectively. Test of quarter level prevalence of
mastitis was done on 484 quarters and it was found that 9.32% (45) and 37.89% (183) of the quarters had clinical and subclinical cases, respectively. The quarter level prevalence of clinical and sub clinical
mastitis from the highest to lowest rate of infection was left front teats (LF) 53.72% (65), right rear teats (RR) 50.00% (60), left rear teats (LR) 45.56% (55) and right front teats (RF) 39.65% (48). Those animals which were affected with tick infestation (χ2 =11.14, p< 0.003),with teat injury (χ2 =7.14, P<0.008) and with history of
mastitis (P<0.003, χ2=8.62) were significantly affected with
mastitis compared to their counter parts. The occurrence of
mastitis based on age, stage of lactation and parity was significantly higher in adult cow (6-9yrs) (χ2 =7.87, P<0.020), in mid (5-7month) lactation (χ2 =6.41, P<0.041), and in cow with moderate (4-7) parity(χ2=11.79, P<0.003) respectively. In conclusion, the hygienic milk production activity in the area as a whole requires an intervention.
Mastitis has been found to be an important disease in the area. This indicates proper
mastitis control should be practiced by maintaining an appropriate cow's environment and udder health management program by the farmers including further investigation on the etiological agents associated with prevalence of
mastitis to undertake measurable control options of
mastitis in the area.
Advisors/Committee Members: Prof. Berhan Tamir (advisor).
Subjects/Keywords: Dairy cow;
hygienic;
mastitis
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yien, D. (2014). ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5547
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yien, Deng. “ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
.” 2014. Thesis, Addis Ababa University. Accessed April 26, 2018.
http://etd.aau.edu.et/dspace/handle/123456789/5547.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yien, Deng. “ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
.” 2014. Web. 26 Apr 2018.
Vancouver:
Yien D. ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
. [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2018 Apr 26].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/5547.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yien D. ASSESSMENT OF HYGIENIC MILK PRODUCTION AND PREVALENCE OF MASTITIS IN DAIRY COW IN JIKAWO WOREDA OF NUER ZONE, GAMBELLA REGION, ETHIOPIA
. [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5547
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht
16.
Embden, G.J. van.
Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds.
Degree: 2008, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/33523
► ABSTRACT Organic food production is a growing sector worldwide and anticipates to the consumer growing demand for food produced on a sustainable way, without the…
(more)
▼ ABSTRACT
Organic food production is a growing sector worldwide and anticipates to the consumer growing demand for food produced on a sustainable way, without the use of antibiotics, chemicals and pesticides, and better animal health and welfare. This report compares health and welfare between organic and conventional
dairy farms by a literature review and a field study done in Alberta, Canada, with emphasis on clinical
mastitis incidence, etiology and antibiotic usage. Animal welfare in organic farming is on average better than in conventional
dairy farming, but health is not necessarily better. Organic regulations and management standards cannot guarantee better health because they do not influence management style. In most studies on health and welfare in organic
dairy farming,
mastitis is used as a parameter to measure animal health. In these studies, incidence of clinical
mastitis varied from a lower incidence to a same or even higher incidence rate on organic farms compared to conventional. In several studies, distribution of
mastitis pathogens cultured from cases of clinical
mastitis showed a slight tendency towards more contagious pathogens in organic farms compared to conventional farms. Antibiotic resistance is expected to be lower on organic farms, but no general higher susceptibility of
mastitis pathogens to antimicrobial drugs was proven in any of the studies, except in the USA, where difference in antibiotic usage between organic and conventional
dairy farms is very high.
In the field study, which was performed within the Alberta Organic
Dairy Research Project, all clinical
mastitis cases in organic farms were sampled and cultured over a period of 2 months. Results were then compared with data of conventional farms obtained from a national cohort study about udder health, performed by the Canadian Bovine
Mastitis Research Network (CBMRN). Unfortunately, no associations with production method (organic vs. conventional) could be made for incidence and etiology of clinical
mastitis and antibiotic resistance at this time, because of the low number of samples collected so far.
Abbreviation key: CNS = coagulase-negative staphylococci, CON = conventional, IRCM = incidence rate of clinical
mastitis, ORG = organic
Advisors/Committee Members: Jorritsma, R..
Subjects/Keywords: Diergeneeskunde; mastitis, organic dairy
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Embden, G. J. v. (2008). Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/33523
Chicago Manual of Style (16th Edition):
Embden, G J van. “Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds.” 2008. Masters Thesis, Universiteit Utrecht. Accessed April 26, 2018.
http://dspace.library.uu.nl:8080/handle/1874/33523.
MLA Handbook (7th Edition):
Embden, G J van. “Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds.” 2008. Web. 26 Apr 2018.
Vancouver:
Embden GJv. Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds. [Internet] [Masters thesis]. Universiteit Utrecht; 2008. [cited 2018 Apr 26].
Available from: http://dspace.library.uu.nl:8080/handle/1874/33523.
Council of Science Editors:
Embden GJv. Clinical mastitis: Incidence, etiology and treatment in organic and conventional dairy herds. [Masters Thesis]. Universiteit Utrecht; 2008. Available from: http://dspace.library.uu.nl:8080/handle/1874/33523

Swedish University of Agricultural Sciences
17.
Ã…kerstedt, Maria.
Bovine acute phase proteins in milk.
Degree: 2008, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1735/
► The composition and quality of the raw milk is essential for the dairy industry and since only healthy cows produce milk of high quality, it…
(more)
▼ The composition and quality of the raw milk is essential for the dairy industry and since only healthy cows produce milk of high quality, it is important with prospering cows. The most important and common disease among dairy cows is mastitis (inflammation of the udder). Mastitis is not only an animal welfare problem, but also results in impaired milk quality, reduced product yield, higher production costs and consequently a higher price for the consumer. The most common way to detect subclinical mastitis is by measuring the somatic cell count (SCC). The SCC is also an important parameter in milk payment systems, highly affecting the price to the producer. Since SCC is influenced by other factors than mastitis, e.g. lactation number, stage of lactation, stress etc., there is a need for new biomarkers for detection of subclinical mastitis as well as for raw milk quality. The aim of this thesis was to obtain further knowledge about the occurrence of the two major acute phase proteins (APP) in bovine milk, haptoglobin (Hp) and serum amyloid A (SAA), and to evaluate their potential as biomarkers for raw milk quality. For the first time, a method for analysis of Hp using an optical biosensor based on surface plasmon resonance (SPR) technology was developed. The method is fully automated with no need for sample preparation before analysis and each sample requires approximately 8 minutes. The occurrence of Hp and SAA in quarter, cow composite and bulk tank milk samples were investigated and the results showed that APP could be detected in all types of samples. In general, detectable levels of APP in milk were related to high SCC, probably originating from cows with subclinical mastitis. In general, samples containing APP had lower casein content, casein number (casein in relation to total protein) and lactose but also increased whey protein content or increased proteolysis. Hp and SAA were suggested to be useful biomarkers for milk quality, especially the protein quality. To our knowledge, this thesis is the first describing Hp and SAA as potential biomarkers for raw milk quality.
Subjects/Keywords: dairy cows; cow milk; quality; proximate composition; proteins; casein; somatic cell count; mastitis; biosensors; proteolysis; bovine; dairy cow; milk quality; acute phase proteins; haptoglobin; serum amyloid A; somatic cell count; mastitis; protein composition; casein content; proteolysis
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ã…kerstedt, M. (2008). Bovine acute phase proteins in milk. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1735/
Chicago Manual of Style (16th Edition):
Ã…kerstedt, Maria. “Bovine acute phase proteins in milk.” 2008. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/1735/.
MLA Handbook (7th Edition):
Ã…kerstedt, Maria. “Bovine acute phase proteins in milk.” 2008. Web. 26 Apr 2018.
Vancouver:
Ã…kerstedt M. Bovine acute phase proteins in milk. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2008. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/1735/.
Council of Science Editors:
Ã…kerstedt M. Bovine acute phase proteins in milk. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2008. Available from: http://pub.epsilon.slu.se/1735/
18.
Fagundes, Helena.
Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo.
Degree: PhD, Qualidade e Produtividade Animal, 2007, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-24042007-103157/
;
► O objetivo deste estudo foi verificar a ocorrência de S. aureus e E. coli O157: H7 no leite de vacas com mastite subclÃnica e no…
(more)
▼ O objetivo deste estudo foi verificar a ocorrência de S. aureus e E. coli O157: H7 no leite de vacas com mastite subclÃnica e no leite de mistura de 42 propriedades leiteiras localizadas em duas regiões do Estado de São Paulo: São Carlos e Ribeirão Preto. Paralelamente, entre os isolados de S. aureus foi objetivo identificar os produtores de toxinas e determinar sua origem epidemiológica. O isolamento de S. aureus foi realizado em agar Baird-Parker (35ºC, 48h) e a confirmação bioquÃmica através da catalase, coagulase, termonuclease, produção de acetoÃna e fermentação aeróbia da maltose. O isolamento de E. coli O157: H7 foi realizado em agar Sorbitol MacConkey MUG (35ºC, 24h). Para confirmação utilizaram-se as provas do IMVC e sorologia através do kit Soro Anti E. coli O157. Para a detecção da TSST-1 e das enterotoxinas A, B, C e D utilizou-se aglutinação reversa passiva em látex (RPLA). A identificação epidemiológica dos isolados de S. aureus foi realizada por eletroforese em gel de campo pulsado (PFGE). A ocorrência de S. aureus no leite individual nas regiões 1 (São Carlos) e 2 (Ribeirão Preto) foram 3,9% e 6,7%, respectivamente. Animais pertencentes à s propriedades leiteiras com produção entre 400 L e 1.000 L/dia apresentaram maior risco de veiculação de S. aureus através do leite quando comparadas com propriedades com produção 1.000 L/dia. Quanto ao leite de mistura, verificou-se que a ocorrência de S. aureus foi a mesma em ambas as regiões (19%). A produção simultânea das enterotoxinas B e C foi observada em 4,7% dos isolados de leite individual, enquanto que 4,7% produziram enterotoxina A e toxina TSST-1. A produção de TSST-1, isoladamente, foi constatada em 14,3% dos isolados de leite individual e em 25% do leite de mistura. Houve similaridade genética entre os isolados de S. aureus, evidenciando sua dispersão epidemiológica entre as propriedades avaliadas. A ocorrência de E. coli O157: H7 no leite individual foi 1% na região 1 e 1,6% na região 2. No leite de mistura não foi detectada E. coli O157: H7. Ressalte-se a importância de medidas preventivas para assegurar a qualidade do leite durante a ordenha, a fim de evitar a ocorrência de microrganismos patogênicos, principalmente S. aureus, e conseqüentemente prevenir riscos de veiculação de toxinfecções através deste alimento.
The aim of this study was to verify the occurrence of S. aureus and E. coli O157: H7 in the milk from dairy cows with subclinical mastitis and in the bulk milk from 42 dairy farms located in two regions of São Paulo State (Region 1: São Carlos, Region 2: Ribeirão Preto). Among the S. aureus strains isolated, the aim was to identify the toxin producers and their epidemiological origin. The isolation of S. aureus was conducted using Baird-Parker agar, and the strains were confirmed by catalase, coagulase, thermonuclease, maltose aerobic fermentation and acetoin production. The isolation of E. coli O157: H7 was conducted using Sorbitol MacConkey MUG agar. The strains were confirmed by IMVC and serology using anti E. coli O157 sera.…
Advisors/Committee Members: Oliveira, Carlos Augusto Fernandes de.
Subjects/Keywords: E. coli O157: H7; Escherichia coli O157: H7; S. aureus; Staphylococcus aureus; Dairy cows; Mastite; Mastitis; Milk quality; Public Health; Qualidade do leite; Saúde Pública; Vacas leiteiras
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Fagundes, H. (2007). Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/74/74131/tde-24042007-103157/ ;
Chicago Manual of Style (16th Edition):
Fagundes, Helena. “Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo.” 2007. Doctoral Dissertation, University of São Paulo. Accessed April 26, 2018.
http://www.teses.usp.br/teses/disponiveis/74/74131/tde-24042007-103157/ ;.
MLA Handbook (7th Edition):
Fagundes, Helena. “Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo.” 2007. Web. 26 Apr 2018.
Vancouver:
Fagundes H. Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2018 Apr 26].
Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-24042007-103157/ ;.
Council of Science Editors:
Fagundes H. Ocorrência de Staphylococcus aureus e Escherichia coli O157:H7 em rebanhos leiteiros do Estado de São Paulo. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/74/74131/tde-24042007-103157/ ;

The Ohio State University
19.
da Costa, Luciana B.
Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms.
Degree: PhD, Comparative and Veterinary Medicine, 2014, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408657108
► Staphylococcus aureus (Staph.aureus) is the most common contagious pathogen causing intramammary infections (IMI) in cows worldwide. Although practices to control this organism have been advocated…
(more)
▼ Staphylococcus aureus (Staph.aureus) is the most
common contagious pathogen causing intramammary infections (IMI) in
cows worldwide. Although practices to control this organism have
been advocated for decades, identification of risk factors is
crucial in prevention and control of Staph.aureus. The objectives
for Chapter 2 and Chapter 3 were to estimate prevalence of
Staph.aureus in Ohio dairies and to determine the association of
management practices with isolation of Staph.aureus in bulk tank
milk (BTM). Questionnaire about herd characteristics, milking
procedures, udder health/
mastitis control, biosecurity and
calf/heifer raising practices were mailed to 780
dairy producers,
with a response rate of 49.2%. Staph.aureus prevalence was 48%, 64%
and 69% when considering one, two or three BTM samples. Herds with
somatic cell count lower than 150,000 cells/mL and milking routine
practices such as pre-stripping, pre- and post-dipping and use of
single towels per cow were associated with reduced detection of
Staph.aureus. Dry-off practices such as abrupt cessation of
milking, use of internal teat sealant or blanket dry treatment were
associated with herd size. In addition to the presence of
Staph.aureus in the infected udder and milk, the cow can also be
colonized with the organism on body sites. The objective of the
Chapter 4 was to assess the role of teat skin colonization by
Staph. aureus in Staph.aureus IMI by evaluating genetic relatedness
of Staph.aureus isolates from milk and teat skin of
dairy cows
using pulsed-field gel electrophoresis (PFGE) and characterizing
the isolates based on the carriage of virulence genes.
Cows in four
known Staph.aureus positive herds were sampled and was found that
quarters with teat skin colonized with Staph.aureus were almost
five times more likely to be diagnosed with Staph.aureus IMI than
quarters not colonized on teat skin. Three main clusters (A, B, C)
were identified with PFGE using a cutoff at 80% similarity. All
clusters contained both milk and teat skin isolates with 72% of
isolates belonging to cluster B. Forty-two virulence factors were
screened using PCR and presence of clfA, clfB genes may have
contributed to the ability of certain isolates to become the
predominant strain. The aim of the study in Chapter 5 was to
estimate antimicrobial resistance in Staph.aureus and to determine
presence of methicillin-resistant Staph.aureus (MRSA) in Ohio
dairy
BTM. Staph.aureus isolates for this study were those previously
described in Chapters 2. Isolates were tested for antimicrobial
resistance by Kirby-Bauer disc diffusion and MRSA confirmation was
done by a duplex PCR using femB and mecA genes. MecA was detected
in two Staph.aureus isolates from a single farm (herd prevalence =
0.95%), collected at different time. Both isolates appeared highly
clonal and belonged to SCC type IV, spa type t021, USA200. These
results confirm the presence in low prevalence of BTM MRSA in US
dairies. Findings from this study show that Staph.aureus IMI is a
multifactorial disease. Characteristics related…
Advisors/Committee Members: Rajala-Schultz, Paivi (Advisor).
Subjects/Keywords: Veterinary Services; Staphylococcus aureus, mastitis, intra-mammary infection,
dairy cows, herd characteristics, management practices, dry-off,
bulk tank milk, virulence factors, PFGE, teat skin colonization,
MRSA, questionnaire, epidemiology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
da Costa, L. B. (2014). Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1408657108
Chicago Manual of Style (16th Edition):
da Costa, Luciana B. “Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms.” 2014. Doctoral Dissertation, The Ohio State University. Accessed April 26, 2018.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1408657108.
MLA Handbook (7th Edition):
da Costa, Luciana B. “Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms.” 2014. Web. 26 Apr 2018.
Vancouver:
da Costa LB. Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms. [Internet] [Doctoral dissertation]. The Ohio State University; 2014. [cited 2018 Apr 26].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408657108.
Council of Science Editors:
da Costa LB. Epidemiology, Genetic and Molecular Characterization of
Staphylococcus aureus in Ohio Dairy Farms. [Doctoral Dissertation]. The Ohio State University; 2014. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1408657108

Swedish University of Agricultural Sciences
20.
Jansson Mörk, Marie.
Validation of disease recordings in Swedish dairy cattle.
Degree: 2009, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/2130/
► This thesis examines the completeness of the Swedish dairy disease recording system: it attempts to quantify how much disease the system’s database captures relative to…
(more)
▼ This thesis examines the completeness of the Swedish dairy disease recording system: it attempts to quantify how much disease the system’s database captures relative to what farmers find and veterinarians treat. Two field studies were conducted. In the first, 177 farmers recorded information about disease events, regardless of whether the disease event had resulted in a veterinary visit. In the second, farm copies of veterinary records (851 records from 112 herds) were collected. The proportion of disease events receiving veterinary treatment was estimated, and measures of disease incidence based on the farmers’ data were compared with incidences estimated from the Dairy Disease Database (ddd). Further, the completeness of the ddd was estimated based on agreement between information in the ddd and farmer-reporting and herd-copies, respectively. Differential completeness was also evaluated. Finally, the probability of a successfully registered disease event for the whole disease recording process was estimated for five different disease complexes, based on the results of both field studies. The overall completeness of veterinary treated disease events in the ddd was estimated to be 71% and 75%, based on the farmers’ recordings and on the farm copies, respectively. Differential completeness linked to regions, veterinary employment type and between different groups of animals was found. The probability of a successfully registered disease event (regardless of veterinary treatment) in the ddd varied between 30% for diarrhoea and 72% for puerperal paresis. Whether or not the farmer contacted a veterinarian was found to be the most influential step in the recording process, followed by whether or not the disease record was registered in the raw data file at the Swedish Dairy Association. Lack of completeness in the ddd will result in conservative disease incidence measures. Underreporting of veterinary treated disease events, as well as undercoverage of farmer-observed events, was found to vary depending on several factors which could introduce bias in estimates based on the ddd, which primarily is a problem if the data are used for epidemiologic research and less so for other areas.
Subjects/Keywords: dairy cows; disease control; epidemiology; monitoring; databases; data collection; data analysis; sweden; dairy cow; epidemiology; disease monitoring system; validity; completeness; sensitivity; differential misclassification; detectable
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APA ·
Chicago ·
MLA ·
Vancouver ·
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APA (6th Edition):
Jansson Mörk, M. (2009). Validation of disease recordings in Swedish dairy cattle. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/2130/
Chicago Manual of Style (16th Edition):
Jansson Mörk, Marie. “Validation of disease recordings in Swedish dairy cattle.” 2009. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/2130/.
MLA Handbook (7th Edition):
Jansson Mörk, Marie. “Validation of disease recordings in Swedish dairy cattle.” 2009. Web. 26 Apr 2018.
Vancouver:
Jansson Mörk M. Validation of disease recordings in Swedish dairy cattle. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2009. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/2130/.
Council of Science Editors:
Jansson Mörk M. Validation of disease recordings in Swedish dairy cattle. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2009. Available from: http://pub.epsilon.slu.se/2130/

University of British Columbia
21.
Proudfoot, Kathryn Louise.
Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
.
Degree: 2008, University of British Columbia
URL: http://hdl.handle.net/2429/2426
► Transition dairy cows are vulnerable to negative consequences of depressed feed intake due to a transient state of negative energy balance that predisposes them to…
(more)
▼ Transition dairy cows are vulnerable to negative consequences of depressed feed intake due to a transient state of negative energy balance that predisposes them to disease after calving. Competition has been identified as one factor that can decrease feeding activity in mid-lactation cows, but the effects of competition on the transition cow are less well understood. The objective of this study was to test the effect of a competition on the behaviour and feed intake of transition cows. Standing behaviour, feeding behaviour and dry matter intake (DMI) was monitored from 1 wk before to 2 wk after calving for 110 Holstein dairy cows. Social behaviour was recorded in the week before calving. Cows were assigned to a competitive (2:1 cows:bin) or non-competitive (1:1 cow:bin) treatment at the start of the study. Treatment groups were balanced for parity and baseline feeding data, resulting in 8 primiparous and 10 multiparous cows per treatment. Competition dramatically increased the number of agonistic behaviours between cows at the feeder. Primiparous cows showed no change in either feeding or standing behaviour when fed in a competitive environment; however, they increased their total meal duration and within-meal intervals in the wk -1 before and wk +1 after calving. In wk -1 before calving, competitively fed multiparous cows increased the frequency of visits to the feeder but consumed less feed at each visit, resulting in decreased daily DMI. Throughout the experiment, multiparous cows fed competitively spent less time eating at each visit and ate at a faster rate, particularly during the 2 wk after calving. Multiparous cows on the competitive treatment also increased the time they spent standing (without eating) compared to cows on the non-competitive treatment. Feeding rate was negatively correlated with social status in multiparous cows. In summary, the results of this study indicate that restricting access to the feeder increases agonistic behaviours regardless of parity, and cows of different parity and social status respond differently in terms of feeding and standing behaviour.
Subjects/Keywords: Competition;
Transition dairy cows;
Behaviour
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Proudfoot, K. L. (2008). Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
. (Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/2426
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Proudfoot, Kathryn Louise. “Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
.” 2008. Thesis, University of British Columbia. Accessed April 26, 2018.
http://hdl.handle.net/2429/2426.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Proudfoot, Kathryn Louise. “Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
.” 2008. Web. 26 Apr 2018.
Vancouver:
Proudfoot KL. Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
. [Internet] [Thesis]. University of British Columbia; 2008. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/2429/2426.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Proudfoot KL. Competition at the feed bunk during transition changes the feeding, standing and social behaviour of Holstein dairy cows
. [Thesis]. University of British Columbia; 2008. Available from: http://hdl.handle.net/2429/2426
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta
22.
Espinoza, Oswaldo S.
Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows.
Degree: MS, Department of Agricultural, Food, and Nutritional
Science, 2016, University of Alberta
URL: https://era.library.ualberta.ca/files/cn583xv04p
► The goals of this research were to evaluate the interaction effects between photoperiod management and dietary grain allocation in lactating dairy cows and to determine…
(more)
▼ The goals of this research were to evaluate the
interaction effects between photoperiod management and dietary
grain allocation in lactating dairy cows and to determine if any
relationships exist between photoperiod management and the
persistency of lactation in selected dairy herds in Alberta. In
study 1, there were no significant interaction effects between
photoperiod management and dietary grain allocation on milk
production, dry matter intake or body weight gain. Cows that were
exposed to long day photoperiod (LP; 16 h of light, 8 h of
darkness) increased milk yield by 2. 2 kg/d relative to the animals
exposed to short day photoperiod (SP; 8 h of light, 16 h of
darkness). However, galactopoietic responses to LP were only
detected four weeks after initial light exposure; when cows were
fed different diets, after adaptation to light treatment, the
effect of LP on milk yield was not detected. Contrarily, cows fed
high grain diets increased milk yield and dry matter intake
compared with those fed low grain diets. In study 2, we found that
animal exposure to light did not differ between summer and winter.
Thus, farms that practiced photoperiod management were able to
provide long day photoperiod throughout the year whereas cows in
farms without photoperiod management were exposed to short
photoperiod even in the summer months. The current study showed
that persistency of lactation was not different for farms with
photoperiod management compared with farms without it. Long day
photoperiod can increase milk production in lactating dairy cows
but animal responses may be affected to a greater extent by other
management practices.
Subjects/Keywords: dairy cows; persistency; photoperiod
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Espinoza, O. S. (2016). Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cn583xv04p
Chicago Manual of Style (16th Edition):
Espinoza, Oswaldo S. “Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows.” 2016. Masters Thesis, University of Alberta. Accessed April 26, 2018.
https://era.library.ualberta.ca/files/cn583xv04p.
MLA Handbook (7th Edition):
Espinoza, Oswaldo S. “Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows.” 2016. Web. 26 Apr 2018.
Vancouver:
Espinoza OS. Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows. [Internet] [Masters thesis]. University of Alberta; 2016. [cited 2018 Apr 26].
Available from: https://era.library.ualberta.ca/files/cn583xv04p.
Council of Science Editors:
Espinoza OS. Effects of Photoperiod Management on Milk Production in
Lactating Dairy Cows. [Masters Thesis]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/cn583xv04p

University of Johannesburg
23.
Staats, Jurgens.
A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
.
Degree: 2009, University of Johannesburg
URL: http://hdl.handle.net/10210/2620
Subjects/Keywords: Dairy cows
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Staats, J. (2009). A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
. (Thesis). University of Johannesburg. Retrieved from http://hdl.handle.net/10210/2620
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Staats, Jurgens. “A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
.” 2009. Thesis, University of Johannesburg. Accessed April 26, 2018.
http://hdl.handle.net/10210/2620.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Staats, Jurgens. “A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
.” 2009. Web. 26 Apr 2018.
Vancouver:
Staats J. A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
. [Internet] [Thesis]. University of Johannesburg; 2009. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/10210/2620.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Staats J. A comparative study on the effect of China officinalis 12CH and China officinalis 12CH-parathyroid 4CH complex on blood calcium levels of multiparous dairy cows
. [Thesis]. University of Johannesburg; 2009. Available from: http://hdl.handle.net/10210/2620
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University
24.
Tekippe, Jessica.
NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS.
Degree: MS, Animal Science, 2010, Penn State University
URL: https://etda.libraries.psu.edu/catalog/10656
► In experiment 1 medicinal herbs and essential oils were evaluated in a batch culture in vitro screening experiment as potential anti-methanogenic additives for ruminant diets.…
(more)
▼ In experiment 1 medicinal herbs and essential oils
were evaluated in a batch culture in vitro screening experiment as
potential anti-methanogenic additives for ruminant diets. A total
of 88 essential oils and 14 herbs were tested. Rumen inoculum
enriched with particle-associated microorganisms was collected from
a lactating dairy cow, 2 h before feeding. Incubation was conducted
in serum bottles containing 1 g of a feed mixture (0.7 g alfalfa
hay, 0.2 g corn starch, and 0.1 g solvent-extracted soybean meal),
1 ml of essential oils (10, 50, and 100 mg/L, final media
concentration), 19 ml of McDougall¡¦s buffer with 5 g/L glucose and
2.5 g/L acid-hydrolyzed casein, and 20 ml of ruminal inoculum.
Bottles were then flushed with CO2 and incubated at 39„aC for 6 h.
Corresponding 50 ml tubes were incubated for 24 h for NDF
degradability analysis. Herbs were tested using the same procedure
except a portion of the alfalfa hay was replaced with 12.5, 50,
100, and 200 mg of herbs (air-dry basis), and NDF was tested using
the DAISY apparatus. Blanks and monensin (5 mg/L, final media
concentration) were also incubated. At the end of the incubations,
total gas and methane production, VFA and ammonia concentration,
and NDF degradability were measured. Treatment by application level
interactions were not significant for any of the essential oil
treatments. Two of the essential oils increased acetate production,
12 increased propionate production, 10 increased butyrate
production, 3 reduced methane production (by 20 to 30%), and 2
decreased ammonia production. With the herbs, 1 increased acetate
production, 2 increased propionate production, 1 decreased methane
production (by 30%), and 1 decreased ammonia production. Eight of
the herbs increased NDF degradability at various inclusion levels.
Overall, these results indicated that some essential oils and
medicinal herbs may have potential to impact ruminal fermentation
in vivo. In experiment 2 there were two cow trials conducted to
study the effects of dietary addition of an essential oil product,
Xtract 6965 (0 vs. 525 mg/d; EO Pancosama) on ruminal fermentation,
total tract digestibility, methane production, manure gas
emissions, N losses, and dairy cow performance. In both experiments
the EO product was added in the vitamin mineral premix. In trial 1,
20 multiparious Holstein cows (4 of which were ruminally
cannulated) were used in a switch over design with 2, 28-d periods.
Cows averaged 47 kg/day milk yield (¡Ó7.1 SD), 63 DIM (¡Ó21.4 SD),
and 656 kg BW (¡Ó75 SD). Average DMI was 27 kg/d (¡Ó 0.59 SD) and
did not differ between treatments (P=0.31). Apparent total tract
digestibly of DM, OM, NDF, ADF, crude protein, and total
nonstructural carbohydrates were similar between treatments (P =
0.16 to 0.90). Rumen pH and concentration of total VFA, acetate,
propionate, butyrate, and valerate, A:P ratio,
polysaccharide-degrading activities, and total free amino acid
concentration were not affected by treatment (P= 0.15 to 0.97).
Isobutyrate (1.12 vs. 0.94 mmol/L; P < 0.0001), isovalerate…
Subjects/Keywords: methane; dairy cows; rumen
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tekippe, J. (2010). NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS. (Masters Thesis). Penn State University. Retrieved from https://etda.libraries.psu.edu/catalog/10656
Chicago Manual of Style (16th Edition):
Tekippe, Jessica. “NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS.” 2010. Masters Thesis, Penn State University. Accessed April 26, 2018.
https://etda.libraries.psu.edu/catalog/10656.
MLA Handbook (7th Edition):
Tekippe, Jessica. “NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS.” 2010. Web. 26 Apr 2018.
Vancouver:
Tekippe J. NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS. [Internet] [Masters thesis]. Penn State University; 2010. [cited 2018 Apr 26].
Available from: https://etda.libraries.psu.edu/catalog/10656.
Council of Science Editors:
Tekippe J. NATURAL BIOACTIVE PRODUCTS AS RUMEN FERMENTATION
MODIFIERS. [Masters Thesis]. Penn State University; 2010. Available from: https://etda.libraries.psu.edu/catalog/10656

University of Illinois – Urbana-Champaign
25.
Khan, Muhammad.
Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period.
Degree: PhD, 0002, 2013, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/45552
► The transition period in dairy cows is the most crucial time for both cow health and dairy management. Three experiments were conducted on transition dairy…
(more)
▼ The transition period in
dairy cows is the most crucial time for both cow health and
dairy management. Three experiments were conducted on transition
dairy cows to evaluate the effects of prepartum overfeeding and lipid supplementation around parturition on hepatic mRNA expression using qPCR and microarray techniques.
In the first experiment, we examined the expression of 44 genes associated with PPARα target genes including fatty acid oxidation, TAG metabolism and storage, potential related nuclear receptors (NRs) and FGF21 modulating GH/IGF signaling in
cows (n = 6/diet) assigned to a control (CON; NEL = 1.34 Mcal/kg DM) or moderate-energy (OVE; NEL = 1.62 Mcal/kg DM) diet during the entire dry period. All
cows were fed a common lactation diet (NEL = 1.69 Mcal/kg DM) postcalving. Percutaneous liver biopsies were collected at -14, 7, 14, and 30 days relative to parturition (DIM) for transcript profiling via quantitative PCR. Estimated prepartal energy balance (EBAL) was greater (~159% vs. 102%, P<0.05) in OVE vs. CON, but during the first wk postpartum
cows fed OVE prepartum were in more negative EBAL. Prior to calving, CON
cows had greater (P < 0.05) serum FGF21, which corresponded with greater (P < 0.05) liver FGF21 expression. Concentration of FGF21 decreased (P < 0.05) gradually postpartum regardless of diet. Along with more severe negative EBAL,
cows fed OVE vs. CON prepartum had greater (P < 0.05) postpartal concentrations of NEFA, BHBA, and GH in serum and liver triglyceride. Those data agreed with greater expression of ACOX1, CPT1A, ACADVL, HMGCS2, FGF21, and ANGPTL4 in OVE vs. CON at 7-14 DIM. Despite the gradual increase in serum GH after calving, at 7-14 DIM liver from
cows fed OVE prepartum had greater (P < 0.05) IGFALS potentially to counteract the temporal decrease in hepatic GHR, STAT5ab, and IGF-1. Our results revealed transcriptional adaptations in liver resulting from the link between prepartal energy overfeeding and postpartal negative EBAL leading to greater serum NEFA. . Transcriptional changes encompassed not only LCFA oxidation and GH signaling but also hepatokine production.
In the second experiment, we examined blood metabolites and expression of 58 genes related to inflammation and ER stress in the same
cows assigned (n = 6/diet) to CON or OVE diets during the entire dry period. All
cows were fed a common lactation diet (NEL = 1.69 Mcal/kg) postpartum. Blood was collected on d (± 3) -14, -5, -2, -1, 0, 1, 2, 5, 7, 10, 14 and 21 relative to parturition. Percutaneous liver tissue biopsies were harvested at -14, 7, 14, and 30 d relative to parturition for transcript profiling via quantitative PCR. Estimated prepartal energy balance (EBAL) was greater (P < 0.05) for OVE and averaged 159% of requirements compared with 102% in CON. However, EBAL during the first week postpartum was lower in OVE (83% vs. 89% of requirements). After parturition the concentrations of ceruloplasmin, creatinine, bilirubin and reactive oxygen metabolites (ROM) were greater (Diet × Time; P < 0.05) in OVE. Around…
Advisors/Committee Members: Loor, Juan J. (advisor), Loor, Juan J. (Committee Chair), Drackley, James K. (committee member), Hurley, Walter L. (committee member), Pan, Yuan-Xiang (committee member).
Subjects/Keywords: transition period; transcriptome; Dairy cows
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Khan, M. (2013). Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/45552
Chicago Manual of Style (16th Edition):
Khan, Muhammad. “Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 26, 2018.
http://hdl.handle.net/2142/45552.
MLA Handbook (7th Edition):
Khan, Muhammad. “Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period.” 2013. Web. 26 Apr 2018.
Vancouver:
Khan M. Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/2142/45552.
Council of Science Editors:
Khan M. Effects of prepartum dietary energy and lipid supplementation on hepatic transcriptome profiles in dairy cows during the transition period. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/45552

Cornell University
26.
Yasui, Takashi.
Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
.
Degree: 2013, Cornell University
URL: http://hdl.handle.net/1813/34369
► Factors associated with a failure to adapt metabolic and physiological regulation during the transition period include oxidative stress, metabolic disorders, and imbalanced energy status. Those…
(more)
▼ Factors associated with a failure to adapt metabolic and physiological regulation during the transition period include oxidative stress, metabolic disorders, and imbalanced energy status. Those factors are not only correlated to each other, but also related to low performance and immune suppression, which leads to development of periparturient-related diseases such as cytological endometritis (CE). The objectives of this work were to investigate the: 1) effect of source of trace minerals on production, oxidative metabolism, and the incidence of CE; 2) effect of chromium propionate (Cr-Pro) supplementation on production, metabolism, and the incidence of CE; 3) effect of supplemental monensin and varying postpartum fermentable starch level on the incidence of CE and related immune functions; and 4) association of CE with energy metabolism and inflammation. The first experiment found that, compared to inorganic or organic sources, supplemental hydroxy trace minerals (HTM), a recently available source of trace minerals, improved aspects of milk production, modulated plasma indices of oxidative balance, and reduced plasma haptoglobin levels immediately after parturition, suggesting that HTM are more bioavailable and can affect the body system related to production, oxidative metabolism, and inflammatory responses. In experiment two, feeding Cr-Pro resulted in improved prepartum dry matter intake in addition to reduced prepartum plasma non-esterified fatty acid (NEFA), and decreased incidence of CE along with increased uterine neutrophil influx immediately after parturition. This implies that Cr-Pro can improve periparturient energy metabolism and uterine immunity. The third experiment revealed that innate immune responses were improved during the early postpartum period in
cows fed high starch diets for 3 weeks after parturition, and during early lactation in
cows fed monensin. Finally, analyses conducted indicated that negative energy balance during the first 3 weeks after parturition was a critical factor to develop CE because
cows with CE had higher area under the curve of plasma NEFA and beta-hydroxybutyric acid, and lower calculated energy balance during that period compared to
cows without CE. The findings obtained from this work give practical approaches to optimize overall performance in transition
dairy cows and provide partial mechanisms for how oxidative status, metabolism, and energy balance interrelate.
Advisors/Committee Members: Nydam, Daryl Van (committeeMember), Gavalchin, Jerrie (committeeMember), Gilbert, Robert Owen (committeeMember), Flaminio, Maria Julia Bevilaqua Felippe (committeeMember).
Subjects/Keywords: Transition dairy cows;
Endometritis;
Nutrition
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yasui, T. (2013). Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/34369
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yasui, Takashi. “Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
.” 2013. Thesis, Cornell University. Accessed April 26, 2018.
http://hdl.handle.net/1813/34369.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yasui, Takashi. “Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
.” 2013. Web. 26 Apr 2018.
Vancouver:
Yasui T. Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
. [Internet] [Thesis]. Cornell University; 2013. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/1813/34369.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yasui T. Nutritional Implications For Oxidative Status, Immune Function, And Energy Metabolism In Transition Dairy Cows And Relationships With Postpartum Performance And Endometritis
. [Thesis]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34369
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University
27.
Machado, Vinicius.
New Insights Into Postpartum Uterine Diseases Of Dairy Cows
.
Degree: 2015, Cornell University
URL: http://hdl.handle.net/1813/40734
► Uterine diseases of dairy cows have a negative impact in the dairy industry because they are prevalent, contribute to economic losses and are an animal…
(more)
▼ Uterine diseases of
dairy cows have a negative impact in the
dairy industry because they are prevalent, contribute to economic losses and are an animal welfare issue. This dissertation was conducted to advance our knowledge of uterine diseases of
dairy cows. A series of studies was conducted to: i) Develop new strategies to better diagnose endometritis, ii) Advance the understanding of the intrauterine microbiota associated with the presence of uterine diseases, iii) Evaluate the association between the cow's immune system and uterine diseases, and iv) Evaluate different strategies to prevent and treat uterine diseases, and improve reproductive performance. Chapter 2 described that uterine lavage sample optical density measured at 620 nm can be used as a diagnostic tool for clinical endometritis. In Chapter 3 and 4, it was reported that intrauterine treatments of mannose or a bacteriophage cocktail against E. coli, and intrauterine infusion of dextrose, were ineffective to prevent or treat uterine diseases. Chapter 9 describes that subcutaneous immunization with components of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevented puerperal metritis. Chapters 5, 6, and 7 describe three studies evaluating the benefits of injectable trace mineral supplementation (ITMS) during the transition period. ITMS improved mammary gland health, decreased clinical endometritis incidence, improved the antioxidant status of
cows, and decreased the intrauterine presence of known intrauterine pathogens. Findings from Chapter 3, 7, and 9 reinforces the importance of E. coli, T. pyogenes, and F. necrophorum as etiological agents of uterine diseases. The first complete genome sequence of Trueperella pyogenes is reported in Chapter 8. Findings from chapter 9 imply that adaptive immunity is playing a role in the uterine defense, and that immunoglobulins are protective against uterine bacterial infection. This is also supported by findings from Chapter 10, which describes that higher blood levels of natural antibodies are positively associated with postpartum uterine health. In summary, this dissertation contributed to the current knowledge on several aspects of postpartum uterine diseases of
dairy cows, but further research is needed to advance the knowledge on the pathogenesis of uterine diseases, and to develop better strategies to prevent, and treat those diseases.
Advisors/Committee Members: Gilbert,Robert Owen (committeeMember), Butler,Walter Ronald (committeeMember), Nydam,Daryl Van (committeeMember).
Subjects/Keywords: Uterine diseases;
Dairy cows;
reproduction
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Machado, V. (2015). New Insights Into Postpartum Uterine Diseases Of Dairy Cows
. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/40734
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Machado, Vinicius. “New Insights Into Postpartum Uterine Diseases Of Dairy Cows
.” 2015. Thesis, Cornell University. Accessed April 26, 2018.
http://hdl.handle.net/1813/40734.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Machado, Vinicius. “New Insights Into Postpartum Uterine Diseases Of Dairy Cows
.” 2015. Web. 26 Apr 2018.
Vancouver:
Machado V. New Insights Into Postpartum Uterine Diseases Of Dairy Cows
. [Internet] [Thesis]. Cornell University; 2015. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/1813/40734.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Machado V. New Insights Into Postpartum Uterine Diseases Of Dairy Cows
. [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40734
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria
28.
Gibbens, Nadine.
Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle.
Degree: Animal and Wildlife
Sciences, 2012, University of Pretoria
URL: http://hdl.handle.net/2263/29303
► Milk fever (parturient paresis / hypocalcaemia) is a metabolic disorder that usually occurs near parturition and at the onset of lactation in high producing multiparous…
(more)
▼ Milk fever (parturient paresis / hypocalcaemia) is a
metabolic disorder that usually occurs near parturition and at the
onset of lactation in high producing multiparous
dairy cows. Milk
fever can indirectly contribute to an increased incidence of
several diseases in early lactation. This study was conducted to
compare two different feeding strategies to prevent milk fever,
namely (i) the established concept of feeding a diet with a
negative DCAD and (ii) a feeding strategy combining a negative DCAD
supplement with 25-hydroxyvitamin D3 (25-OH-D3). Thirty
dairy cows
were used in a randomized block design and were selected and
blocked by parity (second parity and later), 305 day mature
equivalent milk production in the previous lactation and expected
calving date. Within each of the 15 blocks, the
cows were allocated
to two experimental groups named DCAD and DCAD + HyD. Fifteen
animals in the DCAD + HyD group received a daily oral dosage of 3
mg of 25-OH-D3. Plasma samples were collected from day 21 prepartum
to 10 days postpartum and were analysed for 25-OH-D3,
1,25-dihydroxyvitamin D3, total and ionized calcium, phosphorus and
magnesium. Samples were collected on day 21, 14, 10 prepartum and
every second day to calving, 4 and 6 h postpartum and every second
day up to day 10 after calving. Urinary samples for determination
of macro minerals (calcium and phosphorus) were collected via
manual stimulation on day 21, 14, 8 and 4 prepartum and day 4
postpartum. These samples were used to ensure that mild metabolic
acidosis was achieved in both treatment groups. The metabolic
acidosis was demonstrated by decreased urinary pH. Milk samples
were collected on day 1, 4 and 10 postpartum and used for macro
mineral (calcium and phosphorus) determination. This study did not
achieve all of the expected results observed in similar
experiments. No treatment differences could be detected for plasma
Ca2+ concentrations (P>0.05) and the mean plasma Ca2+
concentrations were [1.086a mmol/L ± 0.010 (DCAD treatment) and
1.083a mmol/L ± 0.010 (DCAD + HyD treatment)] respectively.  
Furthermore 1,25-(OH)2D3 plasma concentrations did not indicate any
treatment differences (P>0.05). These results could be due to
the fact that the experimental animals were not sufficiently
challenged and therefore the combination of a low DCAD diet and
Rovimix HyD did not influence the calcium homeostatic mechanisms as
expected. A clear correlation between plasma 25-OH-D3 concentration
and treatment duration was however demonstrated (P<0.001),
indicating effective absorption of orally supplemented 25-OH-D3.
Several authors demonstrated that feeding massive doses of vitamin
D2 (30 million units) for extended periods led to clinical evidence
of vitamin D toxicity. When 10 million IU of vitamin D3 were
however administered intramuscularly within 10 days of parturition,
a reasonable measure of protection against toxicity could be
provided. It can be concluded from this study that longer feeding
periods (± 21 days) than the proposed 10 days prior to calving can
safely…
Advisors/Committee Members: Prof L J Erasmus (advisor).
Subjects/Keywords: Milk
fever; Dairy
cows; Dairy
cattle;
UCTD
Record Details
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Share »
Record Details
Similar Records
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gibbens, N. (2012). Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/29303
Chicago Manual of Style (16th Edition):
Gibbens, Nadine. “Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle.” 2012. Masters Thesis, University of Pretoria. Accessed April 26, 2018.
http://hdl.handle.net/2263/29303.
MLA Handbook (7th Edition):
Gibbens, Nadine. “Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle.” 2012. Web. 26 Apr 2018.
Vancouver:
Gibbens N. Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle. [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2018 Apr 26].
Available from: http://hdl.handle.net/2263/29303.
Council of Science Editors:
Gibbens N. Influence of
25-hydroxyvitamin D and anionic salts on the calcium status of
dairy cattle. [Masters Thesis]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/29303

University of Pretoria
29.
[No author].
Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
.
Degree: 2012, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-11072012-181140/
► Milk fever (parturient paresis / hypocalcaemia) is a metabolic disorder that usually occurs near parturition and at the onset of lactation in high producing multiparous…
(more)
▼ Milk fever (parturient paresis / hypocalcaemia) is a
metabolic disorder that usually occurs near parturition and at the
onset of lactation in high producing multiparous
dairy cows. Milk
fever can indirectly contribute to an increased incidence of
several diseases in early lactation. This study was conducted to
compare two different feeding strategies to prevent milk fever,
namely (i) the established concept of feeding a diet with a
negative DCAD and (ii) a feeding strategy combining a negative DCAD
supplement with 25-hydroxyvitamin D3 (25-OH-D3). Thirty
dairy cows
were used in a randomized block design and were selected and
blocked by parity (second parity and later), 305 day mature
equivalent milk production in the previous lactation and expected
calving date. Within each of the 15 blocks, the
cows were allocated
to two experimental groups named DCAD and DCAD + HyD. Fifteen
animals in the DCAD + HyD group received a daily oral dosage of 3
mg of 25-OH-D3. Plasma samples were collected from day 21 prepartum
to 10 days postpartum and were analysed for 25-OH-D3,
1,25-dihydroxyvitamin D3, total and ionized calcium, phosphorus and
magnesium. Samples were collected on day 21, 14, 10 prepartum and
every second day to calving, 4 and 6 h postpartum and every second
day up to day 10 after calving. Urinary samples for determination
of macro minerals (calcium and phosphorus) were collected via
manual stimulation on day 21, 14, 8 and 4 prepartum and day 4
postpartum. These samples were used to ensure that mild metabolic
acidosis was achieved in both treatment groups. The metabolic
acidosis was demonstrated by decreased urinary pH. Milk samples
were collected on day 1, 4 and 10 postpartum and used for macro
mineral (calcium and phosphorus) determination. This study did not
achieve all of the expected results observed in similar
experiments. No treatment differences could be detected for plasma
Ca2+ concentrations (P>0.05) and the mean plasma Ca2+
concentrations were [1.086a mmol/L ± 0.010 (DCAD treatment) and
1.083a mmol/L ± 0.010 (DCAD + HyD treatment)] respectively.  
Furthermore 1,25-(OH)2D3 plasma concentrations did not indicate any
treatment differences (P>0.05). These results could be due to
the fact that the experimental animals were not sufficiently
challenged and therefore the combination of a low DCAD diet and
Rovimix HyD did not influence the calcium homeostatic mechanisms as
expected. A clear correlation between plasma 25-OH-D3 concentration
and treatment duration was however demonstrated (P<0.001),
indicating effective absorption of orally supplemented 25-OH-D3.
Several authors demonstrated that feeding massive doses of vitamin
D2 (30 million units) for extended periods led to clinical evidence
of vitamin D toxicity. When 10 million IU of vitamin D3 were
however administered intramuscularly within 10 days of parturition,
a reasonable measure of protection against toxicity could be
provided. It can be concluded from this study that longer feeding
periods (± 21 days) than the proposed 10 days prior to calving can
safely…
Advisors/Committee Members: Prof L J Erasmus (advisor).
Subjects/Keywords: Milk fever;
Dairy cows;
Dairy cattle;
UCTD
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share





❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2012). Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-11072012-181140/
Chicago Manual of Style (16th Edition):
author], [No. “Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
.” 2012. Masters Thesis, University of Pretoria. Accessed April 26, 2018.
http://upetd.up.ac.za/thesis/available/etd-11072012-181140/.
MLA Handbook (7th Edition):
author], [No. “Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
.” 2012. Web. 26 Apr 2018.
Vancouver:
author] [. Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
. [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2018 Apr 26].
Available from: http://upetd.up.ac.za/thesis/available/etd-11072012-181140/.
Council of Science Editors:
author] [. Influence of 25-hydroxyvitamin D and anionic salts on
the calcium status of dairy cattle
. [Masters Thesis]. University of Pretoria; 2012. Available from: http://upetd.up.ac.za/thesis/available/etd-11072012-181140/

Swedish University of Agricultural Sciences
30.
Dernfalk, Johanna.
Multiplex flow cytometric assays for markers of inflammation.
Degree: 2008, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1696/
► The aim of this thesis was to develop new techniques for quantification of bovine pro-inflammatory markers, with emphasis on cytokines and acute phase proteins, and…
(more)
▼ The aim of this thesis was to develop new techniques for quantification of bovine pro-inflammatory markers, with emphasis on cytokines and acute phase proteins, and to apply the techniques, using mastitis as disease model. Singleplex, duplex and triplex xMAP assays were developed for the bovine cytokines tumour necrosis factor-alpha (TNF-alpha), interleukin-1β.(IL-1beta), interleukin-6 (IL-6), and the bovine acute phase proteins serum amyloid A (SAA) and lipopolysaccharide binding protein (LBP). Detection limits, linear ranges and intra- and inter-assay variations differed between assays, but generally, lower detection limits and wider linear ranges were observed in singleplex assays than in duplex and triplex assays. The detection limits for TNF-alpha, IL-1beta and IL-6 were satisfactory in the singleplex assays. Further development work is required before the multiplex formats can be used in assays where very low cytokine concentrations are of interest. In the assays for acute phase proteins the detection limits and linear ranges were satisfactory, but could probably be further improved. All xMAP assays could be used for quantification of the analytes in milk and plasma samples from cows with experimentally induced Escherichia coli or Staphylococcus aureus mastitis. Simultaneous detection of IL-1β, SAA and LBP was performed in plasma. LBP is secreted solely during bacterial infections, and cytokines and acute phase proteins are secreted at different phases of the inflammation. Thus a time perspective and information whether the infection is of bacterial origin or not is provided by measuring IL-1β, SAA and LBP simultaneously. In one of the studies, ten cows were grouped as high or low responders for TNF-α, IL-1β and IL-6, based on their cytokine response in an ex vivo whole blood stimulation assay (WBA) with lipopolysaccharide (LPS) and E. coli. After the WBA, the cows were intramammary inoculated with E. coli, and the WBA was evaluated for its usefulness as a predictive tool of the severity of E. coli mastitis. The pre-inoculation WBA with used stimulation doses and incubation times could not predict the severity of an E. coli induced mastitis.
Subjects/Keywords: dairy cows; bovine mastitis; cow milk; blood composition; cytokines; inflammation; immunological techniques; immunity; antibodies; animal diseases; laboratory diagnosis; analytical methods; biochemistry; bovine; multiplex particle based flow cytometry; immunoassay; suspension array; biomarkers; pro-inflammatory cytokines; acute phase proteins; mastitis; whole blood stimulation assay; milk; blood
Record Details
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Share »
Record Details
Similar Records
Cite
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dernfalk, J. (2008). Multiplex flow cytometric assays for markers of inflammation. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1696/
Chicago Manual of Style (16th Edition):
Dernfalk, Johanna. “Multiplex flow cytometric assays for markers of inflammation.” 2008. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed April 26, 2018.
http://pub.epsilon.slu.se/1696/.
MLA Handbook (7th Edition):
Dernfalk, Johanna. “Multiplex flow cytometric assays for markers of inflammation.” 2008. Web. 26 Apr 2018.
Vancouver:
Dernfalk J. Multiplex flow cytometric assays for markers of inflammation. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2008. [cited 2018 Apr 26].
Available from: http://pub.epsilon.slu.se/1696/.
Council of Science Editors:
Dernfalk J. Multiplex flow cytometric assays for markers of inflammation. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2008. Available from: http://pub.epsilon.slu.se/1696/
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