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You searched for subject:(cop operon). Showing records 1 – 2 of 2 total matches.

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1. Fath, Puria Motamed. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.

Degree: Engineering, 2010, University of Borås

In the nature, lots of organism apply different kinds of lights such as flourscence or luminoscence for some purposes such as defence or hunting. Firefly luciferase and Bacterial luciferase are the most famous ones which have been used to design Biosensors or Bioreporters in recent decades. Their applications are so extensive from detecting pollutions in the environment to medical and treatment usages. To design Copper Bioreporter, copper resistance promoter from COP operon which plays an important role in Pseudomonas syringae and pGL3 plasmid which has luciferase gene were utilized. To achieve that target, sequences of promoter were synthesized and inserted to pCR2.1 vector, then suitable primers with considering restriction sites were designed to get high concentration of DNA. After digestion of pGL3 and interested gene by Nhe I and Sac I enzymes, ligation was performed, and then recombinat plasmids were transferred to E. coli BL-21 as a host cell. Finallay, luciferase assay of designed bioreporter was performed by Luminometer in presence of different concentration of CuSO4. The result was maginificant that confirmed design of Copper Bioreporter.

Subjects/Keywords: copper bioreporter; luciferase assay; cop operon; pgl3; e. coli bl-21; Engineering and Technology; Teknik och teknologier

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Fath, P. M. (2010). Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. (Thesis). University of Borås. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Thesis, University of Borås. Accessed November 20, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Web. 20 Nov 2019.

Vancouver:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Internet] [Thesis]. University of Borås; 2010. [cited 2019 Nov 20]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Thesis]. University of Borås; 2010. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of Adelaide

2. Hii, Mei Mei. Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8.

Degree: 2009, University of Adelaide

Sequence analysis of part of a large plasmid carried by Australian environmental isolate of Listeria monocytogenes strain DRDC8 has lead to identification of an islet of genes that encode proteins similar to copper binding and transport genes found in other Gram positive bacteria. Comparative sequence analysis showed that there are at least four genes (pCT0017, pCT0018, pCT0019 and ctpA) on this islet predicted to be involved in copper homeostasis. One of these, ctpA, is predicted to encode a P-type ATPase with a function analogous to CopA, a copper transporting gene in Enterococcus hirae. ORF pCT0017 is likely to be a CopY-like regulatory protein which could control the expression of ctpA. ORF pCT0019 is predicted to be a Cu²⁺ binding protein. In addition, two genes located downstream of the ctpA are predicted to encode a two component regulatory system region. The predicted function of ORF pCT0018 is not clear. A related chromosomal gene (cutR) is predicted to also encode a copper transporting P-type ATPase. To investigate the role of the protein encoded by pCT0018, the growth behavior of L. monocytogenes strain DRDC8, other strains carrying mutations within pCT0018, pCT0019, cutR and ctpA, as well as strains cured of the large plasmid, were grown under conditions of copper stress and starvation. The growth data showed that with the exception of strain DRDC8 and other strains carrying ctpA, most were unable to grow at higher copper concentration (>15 mM CuSO₄) and suggested that the copper homeostasis genes located on the large plasmid are associated with tolerance to high levels of copper. Strain DSE955PL, which carries a cutR mutation and is cured of the large plasmid, was the most sensitive (<5 mM CuSO₄). This indicated that proteins encoded by plasmid genes work synergistically to confer tolerance to copper. Of most interest was the fact that a pCT0018 mutant was more sensitive (<15 mM CuSO₄) to high levels of copper than the wild type parent DRDC8 (<20 mM CuSO₄). This suggested that ORF pCT0018 was necessary for copper tolerance. To investigate the effects of insertion mutations in pCT0017, pCT0018 and ctpA on copper uptake and export, the levels of copper accumulated by these strains was assessed using atomic absorption spectroscopy. A significant difference in copper accumulation among the bacteria strains was observed when either LEB or BHI media were used to culture the bacteria. This data suggested that the growth medium chemicals influence the levels of copper accumulated by cells. However, the effect of these media on bacteria growth rates during copper stress was not significant. Atomic absorption analysis of intracellular copper accumulation suggested that DSE955PL and DSE955 (a chromosome mutant) were able to accumulate copper (80 - 110 mg.gˉ¹ dry weight of cells), whereas DRDC8 and strains carrying mutations in pCT0018, ctpA, and strains cured of the large plasmid, were less able to accumulate copper (30 - 70 mg.gˉ¹ dry weight of cells). This data suggested that… Advisors/Committee Members: Thomas, Connor (advisor), School of Molecular and Biomedical Science (school).

Subjects/Keywords: Listeria monocytogenes; Copper transport; Homeostasis; Cop operon; CopY; CopZ, CtpA; Copper tolerance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hii, M. M. (2009). Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/57906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Hii, Mei Mei. “Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8.” 2009. Thesis, University of Adelaide. Accessed November 20, 2019. http://hdl.handle.net/2440/57906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Hii, Mei Mei. “Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8.” 2009. Web. 20 Nov 2019.

Vancouver:

Hii MM. Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8. [Internet] [Thesis]. University of Adelaide; 2009. [cited 2019 Nov 20]. Available from: http://hdl.handle.net/2440/57906.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hii MM. Role of ORF pCT0018 for copper homeostasis in Listeria monocytogenes strain DRDC8. [Thesis]. University of Adelaide; 2009. Available from: http://hdl.handle.net/2440/57906

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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