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KTH
1.
Zheng, Miaomiao.
Ultrasound Contrast Agents: Fabrication, size distribution and visualization.
Degree: Medical Imaging, 2011, KTH
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152586 
► Ultrasound contrast agents composed of micro-bubble filled with gas are introduced to increase the backscattered power from blood. Their intravenously injection results in the…
(more)
▼ Ultrasound contrast agents composed of micro-bubble filled with gas are introduced to increase the backscattered power from blood. Their intravenously injection results in the improved contrast in the images. The aim of this master thesis project is to manufacture MB suspension at varied temperature and shear forces and to inspect the size distribution and concentration of the PVA-shelled micro-bubble with standard methods according to the developed protocol. A pulser-receiver (Panametrics PR 5072) setup combined with two transducers (2.25 MHz and 5 MHz) was used to investigate the backscattered enhancement of the micro-bubble suspension. Images were collected with transmission optical microscope (OLYMPUS IX71) with the aid of counting chamber. The diameter and concentration of the micro-bubbles were analyzed by Image J. The pulser-receiver setup was used to test the acoustic response. The mean diameter of micro-bubbles was from 2.03 to 4.38 µm with a standard deviation between 0.40 and 1.12 µm and the micro-bubble concentration varied from 0.07× to 5.22× MBs/ml. The enhancement of the ultrasound backscattered power was greater than 20 dB or even reached 30 dB when the energy was increased.
3MiCRON
Subjects/Keywords: Ultrasound; contrast agent; microbubbles
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APA (6th Edition):
Zheng, M. (2011). Ultrasound Contrast Agents: Fabrication, size distribution and visualization. (Thesis). KTH. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152586
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Zheng, Miaomiao. “Ultrasound Contrast Agents: Fabrication, size distribution and visualization.” 2011. Thesis, KTH. Accessed January 16, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152586.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Zheng, Miaomiao. “Ultrasound Contrast Agents: Fabrication, size distribution and visualization.” 2011. Web. 16 Jan 2021.
Vancouver:
Zheng M. Ultrasound Contrast Agents: Fabrication, size distribution and visualization. [Internet] [Thesis]. KTH; 2011. [cited 2021 Jan 16].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152586.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Zheng M. Ultrasound Contrast Agents: Fabrication, size distribution and visualization. [Thesis]. KTH; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152586
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Hellebust, Anne E.
Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues.
Degree: PhD, Engineering, 2016, Rice University
URL: http://hdl.handle.net/1911/88351
► A minimally-invasive, optical strategy to detect and discriminate between inflammation and neoplasia could improve early cancer detection by reducing the number of false positive exams…
(more)
▼ A minimally-invasive, optical strategy to detect and discriminate between inflammation and neoplasia could improve early cancer detection by reducing the number of false positive exams due to benign inflammation. This thesis describes research to optimize optical molecular
contrast agents to observe architectural, metabolic, and biomolecular changes from inflammation and cancer in the gastrointestinal tract. My goal was to: 1) understand the limitations of autofluorescence imaging for cancer detection, 2) image exogenous fluorescent
contrast agents specific to inflammation and neoplasia in rodent models, and 3) topically deliver a
contrast agent cocktail in vivo in a mouse model.
Wide field autofluorescence imaging of oral tissue utilizes endogenous tissue
contrast to discriminate neoplastic from normal tissue; clinical studies of this technique show good sensitivity but poor specificity. I conducted a confocal microscopy study of 47 biopsies from 20 patients; results showed a similar decrease in autofluorescence in the stroma of inflamed and neoplastic tissue. This finding helps explain the low specificity of wide field autofluorescence imaging.
Topically applied exogenous
contrast agents could be used to improve discrimination between neoplasia and inflammation. I tested individual fluorescent
contrast agents and
contrast agent cocktails in chemically induced rodent models of inflammation and neoplasia. The first model used autofluorescence imaging with fluorescence imaging of proflavine to highlight cell nuclei and 2-NBDG to assess metabolic activity for oral cancer detection. A classification algorithm based on proflavine and 2-NBDG staining separated neoplastic from non-neoplastic areas on the tongue with 91% sensitivity and specificity. In the second model, a
contrast agent cocktail composed of proflavine, a fluorescently labelled CD45-targeted antibody to identify inflammatory cells, and permeation enhancers was evaluated for topical in vivo delivery to image ulcerative colitis. The antibody identified the presence of inflammation and established topical delivery of antibody sized agents in vivo.
These results provide evidence that topically applied
contrast agent cocktails could improve discrimination between inflammation and neoplasia when endogenous
contrast is insufficient. An optical-based strategy utilizing
contrast agent cocktails to observe architectural, metabolic, and biomolecular changes associated with inflammation and cancer could improve early cancer detection by reducing the number of false positives from inflammation.
Advisors/Committee Members: Richards-Kortum, Rebecca Rae (advisor), Farach-Carson, Mary (committee member), Gillenwater, Ann (committee member), Sikora, Andrew (committee member), Tkaczyk, Tomasz (committee member).
Subjects/Keywords: cancer; contrast agent; topical delivery
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APA (6th Edition):
Hellebust, A. E. (2016). Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/88351
Chicago Manual of Style (16th Edition):
Hellebust, Anne E. “Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues.” 2016. Doctoral Dissertation, Rice University. Accessed January 16, 2021.
http://hdl.handle.net/1911/88351.
MLA Handbook (7th Edition):
Hellebust, Anne E. “Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues.” 2016. Web. 16 Jan 2021.
Vancouver:
Hellebust AE. Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues. [Internet] [Doctoral dissertation]. Rice University; 2016. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1911/88351.
Council of Science Editors:
Hellebust AE. Optical Contrast Agents to Distinguish Benign Inflammation from Neoplasia in Epithelial Tissues. [Doctoral Dissertation]. Rice University; 2016. Available from: http://hdl.handle.net/1911/88351
University of Minnesota
3.
Weitz, Evan.
Fluorinated Metal Complexes as MRI Contrast Agents.
Degree: PhD, Chemistry, 2015, University of Minnesota
URL: http://hdl.handle.net/11299/191372
► Magnetic resonance imaging (MRI) is a vital tool in today’s modern healthcare system. MRI is preferred over positron emission tomography (PET) and X-ray computed tomography…
(more)
▼ Magnetic resonance imaging (MRI) is a vital tool in today’s modern healthcare system. MRI is preferred over positron emission tomography (PET) and X-ray computed tomography (CT) because it is non-invasive, non-radioactive, and provides 3-D imaging directly in vivo. Contrast agents are used in order to enhance the resolution of the images from MRI. All currently used contrast agents are based on gadolinium and image water protons in the human body. However, gadolinium-based contrast agents are principally unable to quantitatively image specific biomarkers of diseased states, lacking a ratiometric mechanism. Fluorine-based MRI does not suffer from these limitations, but its low sensitivity, with a limit of detection (LOD) in the micromolar range first requires a contrast agent designed specifically to address this issue of sensitivity, which can be accomplished using contrast agents with an incorporated lanthanide center. Fluorine MRI eliminates background signals and has a large chemical shift range which enables fluorines in different environments to each be imaged independently. This in turn allows for the development of ratiometric, responsive contrast agents whereby the total probe concentration and the concentration of the analyte can be independently determined. In this thesis, the theory, practicality, utility, and potential for fluorine-based MRI contrast agents is described. Sensitivity is addressed, synthesis is described, and demonstrations of the potential for fluorine MRI are examined in vitro and in vivo in order to design highly-sensitive, responsive, and biocompatible fluorine contrast agents.
Subjects/Keywords: Contrast Agent; Fluorine; MRI
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APA (6th Edition):
Weitz, E. (2015). Fluorinated Metal Complexes as MRI Contrast Agents. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/191372
Chicago Manual of Style (16th Edition):
Weitz, Evan. “Fluorinated Metal Complexes as MRI Contrast Agents.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 16, 2021.
http://hdl.handle.net/11299/191372.
MLA Handbook (7th Edition):
Weitz, Evan. “Fluorinated Metal Complexes as MRI Contrast Agents.” 2015. Web. 16 Jan 2021.
Vancouver:
Weitz E. Fluorinated Metal Complexes as MRI Contrast Agents. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/11299/191372.
Council of Science Editors:
Weitz E. Fluorinated Metal Complexes as MRI Contrast Agents. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/191372
Université de Grenoble
4.
Tallec, Gaylord.
Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents.
Degree: Docteur es, Chimie, 2011, Université de Grenoble
URL: http://www.theses.fr/2011GRENV051
► L'imagerie par résonance magnétique est une des méthodes de diagnostic les plus utilisées, aussi bien dans le domaine médical que dans les études précliniques. Cependant,…
(more)
▼ L'imagerie par résonance magnétique est une des méthodes de diagnostic les plus utilisées, aussi bien dans le domaine médical que dans les études précliniques. Cependant, la relaxivité des agents de contraste commerciaux ne représente qu'une fraction de la relaxivité prédite par la théorie et il est nécessaire d'optimiser les différents paramètres dont elle dépend pour atteindre des valeurs de relaxivité plus élevées : nombre de molécules d'eau en première sphère de coordination, vitesse d'échange de l'eau, dynamique de rotation du complexe, relaxation électronique, distance Gd(III)-proton. Dans ce travail, nous présentons la synthèse, la stabilité et la relaxivité des complexes de Gd(III) de deux séries de ligands tripodes dérivés de la 8-hydroxyquinoléine, basés l'une sur une plateforme 1,4,7 triazacyclononane, l'autre sur un pivot azote central. Ces complexes ont montré des stabilités comparables à celles des agents commerciaux, des valeurs de relaxivités élevées dans l'eau ainsi qu'en milieu biologique. L'utilisation de la 8-hydroxyquinoléine comme base des ligands a permis de sensibiliser le Nd(III) et l'Yb(III) pour la luminescence proche infrarouge, ouvrant la possibilité pour le développement de nouveaux systèmes bimodaux.
Magnetic resonance imaging is a commonly used diagnostic method in medicinal practice as well as in biological and preclinical research. However, the relaxivity of commercial contrast agents is only a few percent of the theoretically predicted relaxivity. An optimisation of the different parameters who have an impact on the relaxivity (number of gadolinium bound water molecules, water exchange rate, rotation dynamic of the complex, electronic relaxation, Gd(III)-proton distance) is needed to obtain higher relaxivities. In this work, we present the synthesis, the stability and the relaxivity of the Gd(III) complexes of two series of 8-hydroxyquinolinate-based ligands, one using a 1,4,7 triazacyclononane platform, the other one using a central nitrogen architecture. Theses complexes show stabilities comparable to commercial agents, and high relaxivities in both water and serum. The 8-hydroxyquinolinate moiety allows these ligands to sensitize Nd(III) and Yb(III) for Near Infra Red (NIR) luminescence, leading to a new class of potential bimodal systems.
Advisors/Committee Members: Mazzanti, Marinella (thesis director).
Subjects/Keywords: Gadolinium; Agent de contrast; IRM; Luminescence; Gadolinium; Contrast agent; MRI; Luminescence
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APA (6th Edition):
Tallec, G. (2011). Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2011GRENV051
Chicago Manual of Style (16th Edition):
Tallec, Gaylord. “Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents.” 2011. Doctoral Dissertation, Université de Grenoble. Accessed January 16, 2021.
http://www.theses.fr/2011GRENV051.
MLA Handbook (7th Edition):
Tallec, Gaylord. “Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents.” 2011. Web. 16 Jan 2021.
Vancouver:
Tallec G. Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents. [Internet] [Doctoral dissertation]. Université de Grenoble; 2011. [cited 2021 Jan 16].
Available from: http://www.theses.fr/2011GRENV051.
Council of Science Editors:
Tallec G. Nouveaux complexes de lanthanides pour le développement d'agents de contraste bimodaux IRM/luminescence : New lanthanides complexes for the development of bimodal MRI/NIR luminescence contrast agents. [Doctoral Dissertation]. Université de Grenoble; 2011. Available from: http://www.theses.fr/2011GRENV051
Georgia Tech
5.
Sun, In-Cheol.
The development of advanced contrast agents for ultrasound and photoacoustic imaging.
Degree: PhD, Biomedical Engineering (Joint GT/Emory Department), 2018, Georgia Tech
URL: http://hdl.handle.net/1853/61598
► Light and sound are the ones of major energy sources used in the biomedical field throughout history. Today, these energy sources provide foundation for optical,…
(more)
▼ Light and sound are the ones of major energy sources used in the biomedical field throughout history. Today, these energy sources provide foundation for optical, ultrasound, and hybrid imaging technologies. Even though ultrasound and optical imaging techniques alone have various advantages, their applications are often limited by their disadvantages. For example, optical imaging has a low penetration depth beyond which the spatial resolution is drastically reduced; ultrasound imaging has the extended penetration depth and spatial/temporal resolution, but also low
contrast. The combination of light and sound can overcome these limitations because optical and ultrasound imaging have high
contrast and a deep penetration depth, respectively. I developed photoacoustic and ultrasound
contrast agents to enable and assist combined light and ultrasound multimodal imaging. The developed photoacoustic
contrast agents consist of gold nanoparticles, whose surface was modified with a biocompatible polymer, glycol chitosan. Gold nanoparticles absorbed light and generated photoacoustic signals; the surface coating polymer played an important role in the stability and functionality of gold nanoparticles in target sites. These glycol-chitosan-coated gold nanoparticles were applied to cancer cell imaging and lymph node mapping. After the selective accumulation of gold nanoparticles in the cancer cells or lymph nodes, gold nanoparticles produced enhanced photoacoustic signals through the plasmon coupling effect. Both in vitro and in vivo photoacoustic imaging confirmed the feasibility of glycol-chitosan coated gold nanoparticles as a photoacoustic
contrast agent. The developed ultrasound
contrast agents were composed of gold nanoparticles as a photocatalyst and azide compounds as a gas-generating precursor. If the gold nanoparticles were irradiated by a laser, they produced photo-induced electrons for the photolysis of azide compounds, which released nitrogen gas molecules. By these generated N2 bubbles, the enhanced
contrast was achieved in ultrasound imaging. Based on these results, gas-generating gold nanorods were developed for ultrasound and photoacoustic multimodal imaging in in vivo applications. Because of the high absorption coefficient and the tunable surface plasmon resonance peak, gold nanorods have been a promising photoacoustic
contrast agent. Moreover, these unique optical properties also enable photocatalytic reduction of azide groups with near IR laser. The developed azide-conjugated gold nanorods successfully generated enhanced photoacoustic and ultrasound signals during in vivo imaging. The developed photoacoustic and ultrasound
contrast agents have synergistic effects by the combination of two energy sources into one imaging modality. As a result, both
contrast-
agent-aided photoacoustic imaging and laser-induced
contrast-enhanced ultrasound imaging techniques became possible. These new imaging techniques may overcome the limitations that conventional optical and ultrasound imaging have.
Advisors/Committee Members: Emelianov, Stanislav (advisor), Xia, Younan (committee member), Dixon, Brandon (committee member), Robles, Francisco (committee member), El-Sayed, Mostafa (committee member), Kane, Ravi (committee member).
Subjects/Keywords: Photoacoustic contrast agent; Ultrasound contrast agent; Gold nanoparticles; Gas-generating particle
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APA (6th Edition):
Sun, I. (2018). The development of advanced contrast agents for ultrasound and photoacoustic imaging. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/61598
Chicago Manual of Style (16th Edition):
Sun, In-Cheol. “The development of advanced contrast agents for ultrasound and photoacoustic imaging.” 2018. Doctoral Dissertation, Georgia Tech. Accessed January 16, 2021.
http://hdl.handle.net/1853/61598.
MLA Handbook (7th Edition):
Sun, In-Cheol. “The development of advanced contrast agents for ultrasound and photoacoustic imaging.” 2018. Web. 16 Jan 2021.
Vancouver:
Sun I. The development of advanced contrast agents for ultrasound and photoacoustic imaging. [Internet] [Doctoral dissertation]. Georgia Tech; 2018. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1853/61598.
Council of Science Editors:
Sun I. The development of advanced contrast agents for ultrasound and photoacoustic imaging. [Doctoral Dissertation]. Georgia Tech; 2018. Available from: http://hdl.handle.net/1853/61598
University of Louisville
6.
El-Baz, Nagwa.
A cancer-targeted gold nanoparticle-based MRI contrast agent.
Degree: MS, 2018, University of Louisville
URL: 10.18297/etd/3018
;
https://ir.library.louisville.edu/etd/3018
► In oncology, imaging plays a major role in terms of early detection and treatment of most types of cancer. Magnetic resonance imaging (MRI) is…
(more)
▼ In oncology, imaging plays a major role in terms of early detection and treatment of most types of cancer. Magnetic resonance imaging (MRI) is mostly used for cancer diagnosis due to its excellent
contrast resolution. However, MRI for cancer diagnosis is somewhat limited by its sensitivity. In this thesis, we assessed the ability of theranostic platform consisting of gold nanoparticles functionalized with a cancer targeting aptamer; AS1411 and gadolinium chelate (Dotarem thiol derivative; Gd (III)-DO3A) as a MRI
contrast agent to target malignant tumors by enhancing the MRI
contrast of the detected tumor. The proposed technology is a novel injectable
contrast agent for detection and monitoring of malignancies by MRI. The gold nanoparticle core (GNPs) enhances the pharmacokinetic properties of the proposed
contrast agent, increases its potency, and provides a good way to co-localize the aptamer with the
contrast agent. AS1411 is anti-nucleolin aptamer that binds to nucleolin protein, which is highly expressed in cancer cells, leading to selective accumulation in cancers cells. The ability of the proposed
contrast agent to target cancer cells and enhance MRI
contrast was assessed using MDA-MB-231 triple negative breast cells and MCF-10 human mammary epithelial cells. Moreover we assessed the biodistribution and toxicity of the proposed
contrast agent in vivo. The results presented in this thesis demonstrate the superiority of the proposed
contrast agent with respect to the current commercial
contrast agents (e.g., MultiHance).
Advisors/Committee Members: O’Toole, Martin, Malik, Tariq, Malik, Tariq, Clark, Geoffrey, Frieboes, Hermann, Steinbach-Rankins, Jill.
Subjects/Keywords: MRI; AS1411; cancer; contrast agent; Nanomedicine
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APA (6th Edition):
El-Baz, N. (2018). A cancer-targeted gold nanoparticle-based MRI contrast agent. (Masters Thesis). University of Louisville. Retrieved from 10.18297/etd/3018 ; https://ir.library.louisville.edu/etd/3018
Chicago Manual of Style (16th Edition):
El-Baz, Nagwa. “A cancer-targeted gold nanoparticle-based MRI contrast agent.” 2018. Masters Thesis, University of Louisville. Accessed January 16, 2021.
10.18297/etd/3018 ; https://ir.library.louisville.edu/etd/3018.
MLA Handbook (7th Edition):
El-Baz, Nagwa. “A cancer-targeted gold nanoparticle-based MRI contrast agent.” 2018. Web. 16 Jan 2021.
Vancouver:
El-Baz N. A cancer-targeted gold nanoparticle-based MRI contrast agent. [Internet] [Masters thesis]. University of Louisville; 2018. [cited 2021 Jan 16].
Available from: 10.18297/etd/3018 ; https://ir.library.louisville.edu/etd/3018.
Council of Science Editors:
El-Baz N. A cancer-targeted gold nanoparticle-based MRI contrast agent. [Masters Thesis]. University of Louisville; 2018. Available from: 10.18297/etd/3018 ; https://ir.library.louisville.edu/etd/3018
7.
Borsboom, Jerome.
Advanced detection strategies for ultrasound contrast agents.
Degree: 2005, Erasmus University Medical Center
URL: http://hdl.handle.net/1765/76066
► markdownabstract__Abstract__ Ultrasound contrast agent was discovered serendipitously by Gramiak and Shah in I968 when they injected indocyanine green dye into the heart and observed increased…
(more)
▼ markdownabstract__Abstract__
Ultrasound contrast agent was discovered serendipitously by Gramiak and Shah
in I968 when they injected indocyanine green dye into the heart and observed increased
echogenicity of the blood containing the dye. Small cavitation bubbles that
were formed upon injection of the dye were traced to be the source of the enhanced
echoes. Nowadays, ultrasound contrast agent still consists of small bubbles that are
free flowing in the blood stream. However, as the uncontrolled process of cavitation
and violent collapse is considered harmful for cells and tissue, contrast agent is usually
prepared under controlled conditions outside the body and injected through a vein
where they are taken up into the blood stream and transported to the region under
investigation.
Subjects/Keywords: Ultrasound contrast agent
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APA (6th Edition):
Borsboom, J. (2005). Advanced detection strategies for ultrasound contrast agents. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/76066
Chicago Manual of Style (16th Edition):
Borsboom, Jerome. “Advanced detection strategies for ultrasound contrast agents.” 2005. Doctoral Dissertation, Erasmus University Medical Center. Accessed January 16, 2021.
http://hdl.handle.net/1765/76066.
MLA Handbook (7th Edition):
Borsboom, Jerome. “Advanced detection strategies for ultrasound contrast agents.” 2005. Web. 16 Jan 2021.
Vancouver:
Borsboom J. Advanced detection strategies for ultrasound contrast agents. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 2005. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1765/76066.
Council of Science Editors:
Borsboom J. Advanced detection strategies for ultrasound contrast agents. [Doctoral Dissertation]. Erasmus University Medical Center; 2005. Available from: http://hdl.handle.net/1765/76066
Rice University
8.
Gizzatov, Ayrat.
Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications.
Degree: PhD, Natural Sciences, 2015, Rice University
URL: http://hdl.handle.net/1911/87823
► This dissertation focuses on the development of sp2-carbon- and silicon-based nanomaterials for medical diagnostics and in vivo magnetic field-guided delivery applications. To realize these applications,…
(more)
▼ This dissertation focuses on the development of sp2-carbon- and silicon-based nanomaterials for medical diagnostics and in vivo magnetic field-guided delivery applications. To realize these applications, especially for the development of new in vivo Magnetic Resonance Imaging (MRI)
contrast agents (CAs), high solubility in aqueous media is required. Therefore, this work first details development of a new non-covalent method for the preparation of stable aqueous colloidal solution of surfactant-free sp2-carbon nanostructures, as well as a second rapid covalent functionalization procedure to produce highly-water-dispersible honey-comb carbon nanostructures (ca. 50 mg/mL). Next, highly-water-dispersible graphene nanoribbons and Gd3+ ions were together used to produce a high-performance MRI CA for T1- and T2- weighted imaging. In terms of its relaxivity (r1,2) values, this new CA material outperforms currently-available clinical CAs by up to 16 times for r1 and 21 times for r2. Finally, sub-micrometer discoidal magnetic nanoconstructs have been produced and studied for applications for in vivo magnetic-field-guided delivery into cancerous tumors. The nanoconstructs were produced by confining ultra-small superparamagnetic iron oxide nanoparticles (USPIOs) within mesoporous silicon which produced T2-weighted MRI CA performance 2.5 times greater than for the free USPIOs themselves. Moreover, these nanoconstructs, under the influence of an external magnetic field, collectively cooperated via a new mechanism to amplify accumulation in melanoma tumors of mice. Overall, the results of this dissertation could aid in the rapid translation of these nanotechnologies into the clinic, while, hopefully, also serving as an inspiration for continued research into the field of Medical Nanotechnology.
Advisors/Committee Members: Wilson, Lon J. (advisor), Tour, James M (committee member), Vajtai, Robert (committee member), Decuzzi, Paolo (committee member).
Subjects/Keywords: Carbon; Silicon; Nanomaterials; Medical; MRI; Contrast agent
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APA (6th Edition):
Gizzatov, A. (2015). Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/87823
Chicago Manual of Style (16th Edition):
Gizzatov, Ayrat. “Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications.” 2015. Doctoral Dissertation, Rice University. Accessed January 16, 2021.
http://hdl.handle.net/1911/87823.
MLA Handbook (7th Edition):
Gizzatov, Ayrat. “Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications.” 2015. Web. 16 Jan 2021.
Vancouver:
Gizzatov A. Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications. [Internet] [Doctoral dissertation]. Rice University; 2015. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1911/87823.
Council of Science Editors:
Gizzatov A. Carbon and Silicon Nanomaterials for Medical Nanotechnology Applications. [Doctoral Dissertation]. Rice University; 2015. Available from: http://hdl.handle.net/1911/87823
Rice University
9.
Taheri, Nasim.
Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications.
Degree: PhD, Natural Sciences, 2016, Rice University
URL: http://hdl.handle.net/1911/96531
► Of the recent biomedical studies devoted to the development of medical imaging techniques, magnetic resonance imaging (MRI) has been the subject of increased interest. MRI…
(more)
▼ Of the recent biomedical studies devoted to the development of medical imaging techniques, magnetic resonance imaging (MRI) has been the
subject of increased interest. MRI is a powerful diagnostic tool that offers detailed, high-resolution anatomical information by monitoring the relaxation rate of water protons in the presence of a strong magnetic field. However, two objectives that have yet to be fully achieved with MRI include generating efficient
contrast and enhancing imaging sensitivity to a level that enables differentiating healthy tissue from malignant tissue. Developing an effective MRI
contrast to enhance image quality has therefore become crucial to improving this promising technique.
This thesis examines the performance of surface engineered gadolinium oxide nanocrystals as T1 MRI
contrast agents. Gadolinium oxide nanocrystals are formed at high temperatures in organic solvents and phase transferred into biological media using a novel sulfonic acid copolymer. The surface engineered gadolinium oxide nanocrystals were designed to exploit the plate-like geometry of nanocrystals to form surfaces that are both accessible to water and effective at preventing particle-particle aggregation. The crystals’ anisotropic shape suggests that the gadolinium surface atoms on the thin plate edges can remain uncoated and thus available to water. The relaxivities of these materials are one order of magnitude (15 times) larger than commercial T1
contrast agents and other gadolinium-containing nanoparticles. The magnetic field dependence of their relaxation rates and the relatively weak size dependence of their relaxivity suggest that inner-sphere water relaxation at the edges of the nanocrystal are responsible for the high relaxation rates. These surfaces have significant potential as T1 MRI
contrast agents, offering a non-invasive imaging alternative with numerous applications, including detection and characterization of non-alcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH), and tracking of stem cells in embryos.
As part of this thesis, we introduced surface functionalized gadolinium oxide nanocrystals with three different surface coatings—each with unique characteristics. These materials were chosen because they are stable in relevant biological conditions while posing distinct responses in different environments. We examined the effect of surface coating, salt, and protein on the performance of gadolinium oxide nanocrystals as MRI
contrast agents. We found that their behavior was altered in plasma, implying that surface coating has an important effect on their interactions with proteins. Moreover, we showed that, unlike other studies in deionized water in which relaxivity has a linear dependency, forming strong protein binding enables relaxivity measurement to be dependent on gadolinium concentration.
Finally, we studied the possible cytotoxic effects of gadolinium oxide nanocrystals in vitro and in vivo. Since gadolinium ion is a heavy metal, it is important to ensure that it is shielded with…
Advisors/Committee Members: Colvin, Vicki L. (advisor).
Subjects/Keywords: Gadolinium Oxide Nanocrystals; MRI; Contrast Agent; Biomedical
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APA (6th Edition):
Taheri, N. (2016). Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/96531
Chicago Manual of Style (16th Edition):
Taheri, Nasim. “Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications.” 2016. Doctoral Dissertation, Rice University. Accessed January 16, 2021.
http://hdl.handle.net/1911/96531.
MLA Handbook (7th Edition):
Taheri, Nasim. “Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications.” 2016. Web. 16 Jan 2021.
Vancouver:
Taheri N. Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications. [Internet] [Doctoral dissertation]. Rice University; 2016. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1911/96531.
Council of Science Editors:
Taheri N. Gadolinium Oxide Nanocrystals: Synthesis, Characterization, and Applications. [Doctoral Dissertation]. Rice University; 2016. Available from: http://hdl.handle.net/1911/96531
Clemson University
10.
Nguyen, Christian Cole.
Calcium Selective Polymeric Contrast Agents.
Degree: MS, School of Materials Science and Engineering, 2020, Clemson University
URL: https://tigerprints.clemson.edu/all_theses/3284
► This work employs organic and polymer synthesis to construct a novel polymeric contrast agent for the application of mild traumatic brain injury in which…
(more)
▼ This work employs organic and polymer synthesis to construct a novel polymeric
contrast agent for the application of mild traumatic brain injury in which affects so many lives not only in the united states by also internationally as well. Our approach to tackling this issue is to take advantage of diffuse axial injury and the phenomena of the fluctuation of free calcium ions after trauma occurs. We aim to take advantage of chelation chemistry. For this, we carefully construct a polymeric
contrast agent that contains free carboxylic acid groups that can alternate between coordinating with gadolinium species in our
contrast agent and the free calcium ions. In this way, what is hopefully obtained is an ion response polymeric
contrast agent that can be for medical imaging purposes.
The first proposed idea of our work involves simple free radical polymerization containing carboxylic acids on the backbone of the polymer and a conjugated chelating component, which is vastly like ProHance(gadoteridol) a commercially available
contrast agent. The polymer
contrast agents were then successfully characterized using nuclear magnetic resonance (NMR) and Fourier transform inferred spectroscopy (FTIR) for structural analysis. Furthermore, magnetic resonance imaging (MRI) imagining properties determined using a 3 T coil, and the result were recorded and analyzed. While in later works, a more know calcium chelator 1,2 bis(aminophenoxy)ethane-N, N, N′, N′-tetraacetic acid (BAPTA) is latter introduced as well as more advanced synthetic techniques in hopes better to improve the first design of the original concept.
Advisors/Committee Members: Olin T Mefford, Mark Bolding, Igor Luzinov, Stephen H Foulger.
Subjects/Keywords: Calcium; Contrast agent; MRI; mTBI; Polymeric; Selective
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APA (6th Edition):
Nguyen, C. C. (2020). Calcium Selective Polymeric Contrast Agents. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/3284
Chicago Manual of Style (16th Edition):
Nguyen, Christian Cole. “Calcium Selective Polymeric Contrast Agents.” 2020. Masters Thesis, Clemson University. Accessed January 16, 2021.
https://tigerprints.clemson.edu/all_theses/3284.
MLA Handbook (7th Edition):
Nguyen, Christian Cole. “Calcium Selective Polymeric Contrast Agents.” 2020. Web. 16 Jan 2021.
Vancouver:
Nguyen CC. Calcium Selective Polymeric Contrast Agents. [Internet] [Masters thesis]. Clemson University; 2020. [cited 2021 Jan 16].
Available from: https://tigerprints.clemson.edu/all_theses/3284.
Council of Science Editors:
Nguyen CC. Calcium Selective Polymeric Contrast Agents. [Masters Thesis]. Clemson University; 2020. Available from: https://tigerprints.clemson.edu/all_theses/3284
University of Toronto
11.
Haedicke, Inga Elisabeth.
The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications.
Degree: PhD, 2016, University of Toronto
URL: http://hdl.handle.net/1807/89259
► Among clinical imaging modalities, magnetic resonance imaging (MRI) has several advantages including high spatial resolution, excellent soft tissue contrast and lack of ionizing radiation. To…
(more)
▼ Among clinical imaging modalities, magnetic resonance imaging (MRI) has several advantages including high spatial resolution, excellent soft tissue contrast and lack of ionizing radiation. To improve sensitivity and specificity for disease diagnosis, contrast agents (CAs) are administered. Clinical CAs are primarily Gd-based and are limited by a decrease of relaxivity at high magnetic fields,1 and have been associated with nephrogenic systemic fibrosis (NSF), a severe and debilitating disease.2 Therefore, this thesis explores the development of manganese porphyrins (MnPs) as novel CAs designed for high field MRI applications.
MnPs were chosen because certain MnPs have been shown to exhibit anomalously high T1 relaxivity at high field,3 and are known to form highly stable metal chelates.4 The first section describes the design of a highly efficient Gd-free extracellular fluid CA based on a water-soluble MnP, manganese (III) meso-tetracarboxyl porphyrin (MnTCP), for clinical disease diagnosis (Chapter 2). To the best of our knowledge, MnTCP displays the highest r1 among all known small T1 CAs with molecular weight below 600 Dalton (Da) and was successfully used to detect tumors by dynamic contrast enhanced MRI. More recently, as the field of cell transplantation is rapidly expanding, CAs are needed to label and longitudinally track specific populations of cells in vivo. This led to the design and successful application of a cell-permeable, trappable and esterase-responsive MnP (Chapter 3). Highly efficient MRI labeling of two types of human cells was achieved with a relatively low concentration and short incubation time. The new CA is biocompatible and as far as we know, is the most efficient T1 cell labeling CA available to date. Finally, this thesis focuses on molecular imaging of enzyme activity. The progress towards the design and synthesis of an alkaline phosphatase responsive MnP for MRI and analogous ZnP for fluorescence is described in Chapter 4.
2018-07-08 00:00:00
Advisors/Committee Members: Zhang, Xiao-an, Chemistry.
Subjects/Keywords: Agent; Contrast; Imaging; MRI; Porphyrin; Synthesis; 0485
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APA (6th Edition):
Haedicke, I. E. (2016). The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/89259
Chicago Manual of Style (16th Edition):
Haedicke, Inga Elisabeth. “The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications.” 2016. Doctoral Dissertation, University of Toronto. Accessed January 16, 2021.
http://hdl.handle.net/1807/89259.
MLA Handbook (7th Edition):
Haedicke, Inga Elisabeth. “The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications.” 2016. Web. 16 Jan 2021.
Vancouver:
Haedicke IE. The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications. [Internet] [Doctoral dissertation]. University of Toronto; 2016. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1807/89259.
Council of Science Editors:
Haedicke IE. The Development of MnIII-Porphyrins as T1 Contrast Agents for Biomedical Applications. [Doctoral Dissertation]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/89259
Washington University in St. Louis
12.
Maji, Dolonchampa.
Development and applications of novel fluorescent molecular probe strategies.
Degree: PhD, Biomedical Engineering, 2017, Washington University in St. Louis
URL: https://openscholarship.wustl.edu/eng_etds/242
► Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and…
(more)
▼ Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse
contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable
contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent
contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging.
The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence.
Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo.
An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. …
Advisors/Committee Members: Samuel Achilefu, Mark Anastasio, Gregory M. Lanza, Srikanth Singamaneni, Lihong V. Wang.
Subjects/Keywords: contrast agent; fluorescence; optical imaging; Biomedical
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APA (6th Edition):
Maji, D. (2017). Development and applications of novel fluorescent molecular probe strategies. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/eng_etds/242
Chicago Manual of Style (16th Edition):
Maji, Dolonchampa. “Development and applications of novel fluorescent molecular probe strategies.” 2017. Doctoral Dissertation, Washington University in St. Louis. Accessed January 16, 2021.
https://openscholarship.wustl.edu/eng_etds/242.
MLA Handbook (7th Edition):
Maji, Dolonchampa. “Development and applications of novel fluorescent molecular probe strategies.” 2017. Web. 16 Jan 2021.
Vancouver:
Maji D. Development and applications of novel fluorescent molecular probe strategies. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2017. [cited 2021 Jan 16].
Available from: https://openscholarship.wustl.edu/eng_etds/242.
Council of Science Editors:
Maji D. Development and applications of novel fluorescent molecular probe strategies. [Doctoral Dissertation]. Washington University in St. Louis; 2017. Available from: https://openscholarship.wustl.edu/eng_etds/242
University of Arizona
13.
Cardenas Rodriguez, Julio César.
New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
.
Degree: 2012, University of Arizona
URL: http://hdl.handle.net/10150/222845
► Angiogenesis is a fundamental driver of tumor biology and many other important aspect of human health. Dynamic Contrast Enhanced Magnetic Resonance Imaging (DCE-MRI) has been…
(more)
▼ Angiogenesis is a fundamental driver of tumor biology and many other important aspect of human health. Dynamic
Contrast Enhanced Magnetic Resonance Imaging (DCE-MRI) has been shown to be a valuable biomarker for the indirect assessment of angiogenesis. However, DCE-MRI is very specialized technique that has limitations. In this dissertation new models and
contrast agents to address some of these limitations are presented. Chapter 1 presents an introduction to DCE-MRI, the rationale to asses tumor biology with this technique, the MRI pulses sequences and the standard pharmacokinetic modeling used for the analysis of DCE- MRI data. Chapter 2 describes the application of DCE-MRI to asses the response to the hypoxia-activated drug TH-302. It is shown that DCE-MRI can detect a response after only 24 hours of initiating therapy. In Chapter 3, a new model for the analysis of DCE-MRI is presented, the so-called Linear Reference Region Model (LRRM). This new model improves upon existing models and it was demonstrated that it is ~620 faster than current algorithms and 5 times less sensitive to noise, and more importantly less sensitive to temporal resolution which enables the analysis of DCE-MRI data obtained in the clinical setting, which opens a new area of study in clinical MRI. Chapter 4 describes the extension of the LRRM to estimate the absolute permeability of two fluorinated
contrast agents; we call this approach the Reference
Agent Model (RAM). In order to make this new model an experimental reality, a novel pulse sequence and
contrast agents (CA) for ¹⁹F MRI were developed. Two contributions to the field of DCE-MRI are presented in this chapter, the first simultaneous ¹⁹F-DCE-MRI detection of two fluorinated CA in a mouse model of breast cancer, and the estimation of their relative permeability. RAM eliminates some of the physiological variables that affect DCE-MRI, which may improve its sensitivity and specificity. Finally, new potential applications of LRRM and RAM are discussed in Chapter 5.
Advisors/Committee Members: Pagel, Mark D (advisor), Mash, Eugene (committeemember), Ghosh, Indraneel (committeemember), Trouard, Theodore (committeemember), Backer, Amanda F. (committeemember), Pagel, Mark D. (committeemember).
Subjects/Keywords: Pharmacokinetics;
Chemistry;
Contrast Agent;
DCE-MRI
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APA (6th Edition):
Cardenas Rodriguez, J. C. (2012). New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/222845
Chicago Manual of Style (16th Edition):
Cardenas Rodriguez, Julio César. “New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
.” 2012. Doctoral Dissertation, University of Arizona. Accessed January 16, 2021.
http://hdl.handle.net/10150/222845.
MLA Handbook (7th Edition):
Cardenas Rodriguez, Julio César. “New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
.” 2012. Web. 16 Jan 2021.
Vancouver:
Cardenas Rodriguez JC. New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
. [Internet] [Doctoral dissertation]. University of Arizona; 2012. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10150/222845.
Council of Science Editors:
Cardenas Rodriguez JC. New Models and Contrast Agents for Dynamic Contrast-Enhanced MRI
. [Doctoral Dissertation]. University of Arizona; 2012. Available from: http://hdl.handle.net/10150/222845
University of Arizona
14.
Hingorani, Dina Vinoo.
Developing Responsive MRI Contrast Agents to Study Tumor Biology
.
Degree: 2014, University of Arizona
URL: http://hdl.handle.net/10150/333481
► Enzymes are important biomarkers for determining tumor growth and progression. We have developed two molecules to image enzyme response by catalyCEST MRI. This technology allows…
(more)
▼ Enzymes are important biomarkers for determining tumor growth and progression. We have developed two molecules to image enzyme response by catalyCEST MRI. This technology allows for non-invasive detection of enzymes. A background of importance of measuring enzyme activity and MRI agents developed for this purpose have been covered in Chapter 1. We have synthesized a responsive paramagnetic Chemical Exchange Saturation Transfer (CEST)
agent, called Tm-DO3A-cadaverine. This
contrast agents has been successfully cross-linked to the protein albumin by the enzyme transglutaminase leading to the appearance of CEST at -9.2 ppm. The enzyme catalysis has been validated by measuring chemical exchange rates. We have shown that the position of the CEST peak is influenced by the conformation of the molecule depending on the neighboring amino acids to glutamine. This is the first example to show the appearance of CEST due to formation of a covalent bond. We have also synthesized a diamagnetic CEST
agent with a large chemical shift dispersion to detect cathespin B activity. Upon enzyme mediated cleavage of PheArgSal, the aryl amide CEST peak at 5.3 ppm disappears. Taking a ratio of the CEST effects from salicylic acid at 9.5 ppm and aryl amide at 5.3 ppm we can detect enzyme activity. The salicylic acid moiety also undergoes some slow response due to enzyme action, as evident by the disappearance of CEST at 9.5 ppm. However, this proof of concept study is the first example of a DIACEST
agent designed to measure enzyme activity using a ratio of two CEST effects from the same substrate. The last chapter highlights suggests improvements to the catalyCEST research. The appendix shows the use of bulk magnetic susceptibility measurements by NMR to determine bio-distribution of lanthanides in ex-vivo tissue.
Advisors/Committee Members: Pagel, Mark D (advisor), Pagel, Mark D. (committeemember), Glass, Richard S. (committeemember), Mash, Jr., Eugene A. (committeemember).
Subjects/Keywords: Contrast agent;
Enzymes;
Imaging;
Chemistry;
CEST MRI
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APA (6th Edition):
Hingorani, D. V. (2014). Developing Responsive MRI Contrast Agents to Study Tumor Biology
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/333481
Chicago Manual of Style (16th Edition):
Hingorani, Dina Vinoo. “Developing Responsive MRI Contrast Agents to Study Tumor Biology
.” 2014. Doctoral Dissertation, University of Arizona. Accessed January 16, 2021.
http://hdl.handle.net/10150/333481.
MLA Handbook (7th Edition):
Hingorani, Dina Vinoo. “Developing Responsive MRI Contrast Agents to Study Tumor Biology
.” 2014. Web. 16 Jan 2021.
Vancouver:
Hingorani DV. Developing Responsive MRI Contrast Agents to Study Tumor Biology
. [Internet] [Doctoral dissertation]. University of Arizona; 2014. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10150/333481.
Council of Science Editors:
Hingorani DV. Developing Responsive MRI Contrast Agents to Study Tumor Biology
. [Doctoral Dissertation]. University of Arizona; 2014. Available from: http://hdl.handle.net/10150/333481
Vanderbilt University
15.
Semmineh, Natenael Berhanu.
Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media.
Degree: PhD, Physics, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/12183
► The systematic investigation of susceptibility-induced contrast in MRI is important to improve our understanding of the influence of tissue microstructure on dynamic susceptibility contrast (DSC)-MRI…
(more)
▼ The systematic investigation of susceptibility-induced
contrast in MRI is important to improve our understanding of the influence of tissue microstructure on dynamic susceptibility
contrast (DSC)-MRI derived perfusion data. The Finite Perturber Method (FPM) has previously been used to investigate susceptibility
contrast in MRI arising from arbitrarily shaped structures. However, the FPM has low computational efficiency in simulating water diffusion, especially for complex three-dimensional structures that mimic tissue. In this work, an improved computational approach that combines the FPM with a matrix-based finite difference method (FDM), termed the Finite Perturber Finite Difference Method (FPFDM), was developed to more efficiently investigate the biophysical basis of DSC-MRI data and its sensitivity to vascular geometry and
contrast agent (CA) distribution within tissue. The application of the FPFDM to the physiological and physical conditions encountered in a typical DSC-MRI brain tumor study enabled the derivation of a new DSC-MRI metric, termed the Transverse Relaxivity at Tracer Equilibrium (TRATE), which we propose specifically reports on tumor cellular properties. Computational FPFDM studies revealed that TRATE depends on cellular density, size, shape and spatial distribution. To validate the in vivo sensitivity of TRATE it was evaluated in two animal brain tumor models, the 9L and C6, which have varying cellular characteristics. The TRATE values were also compared to measures of the apparent diffusion coefficient (ADC), the CA transfer constant (Ktrans), the extravascular extracellular volume fraction (ve) and histological data. The TRATE values in 9L tumors were significantly higher than those in C6 tumors, a finding that reflects the histologically confirmed higher cell density in 9L tumors and lower cellular density. A voxel-wise comparison of TRATE with ADC, ve, and Ktrans maps showed low spatial correlation, indicating it is providing unique and complementary information on tumor status. In summary, the studies described herein highlight the value of pairing computational and experimental advancements in order to enhance our characterization of DSC-MRI
contrast mechanisms and how such mechanisms can be leveraged to derive new non-invasive metrics for evaluating brain tumors.
Advisors/Committee Members: Kalman Varga (committee member), David J. Ernst (committee member), John C. Gore (committee member), Daniel F. Gochberg (committee member), C. Chad Quarles (Committee Chair).
Subjects/Keywords: dynamic susceptibility contrast; transverse relaxation; vascular structures; cellular structures; contrast agent leakage; diffusion.
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APA (6th Edition):
Semmineh, N. B. (2014). Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12183
Chicago Manual of Style (16th Edition):
Semmineh, Natenael Berhanu. “Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 16, 2021.
http://hdl.handle.net/1803/12183.
MLA Handbook (7th Edition):
Semmineh, Natenael Berhanu. “Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media.” 2014. Web. 16 Jan 2021.
Vancouver:
Semmineh NB. Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1803/12183.
Council of Science Editors:
Semmineh NB. Computational and Experimental Investigation of DSC-MRI Signal
Behavior in Magnetically Inhomogeneous Media. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/12183
University of Toronto
16.
Tieu, Henry.
Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents.
Degree: 2018, University of Toronto
URL: http://hdl.handle.net/1807/101584
► Magnetic resonance imaging (MRI) routinely uses contrast agents (CAs) to enhance the contrast between native tissue and the target. Currently, gadolinium based CAs (GBCAs) dominate…
(more)
▼ Magnetic resonance imaging (MRI) routinely uses contrast agents (CAs) to enhance the contrast between native tissue and the target. Currently, gadolinium based CAs (GBCAs) dominate the clinical MRI applications. However, GBCAs are limited by their low contrast enhancement efficiency and growing concerns about Gd associated toxicity. To address these limitations, we develop a new family of manganese(III) porphyrin (MnPs) CAs as non-Gd alternatives with improved sensitivity and biocompatibility. In this thesis, we further evaluate the potential of our MnPs as CAs. We expand the use of our blood pool agent, MnP2, to act as an oxygen sensor for MRI (Chapter 2) and for the first time, a potential ratiometric imaging sensor for MRI (Chapter 3). Lastly, we investigate and demonstrate the remarkable high stability of MnTCP against conditions that cause metal dissociation in GBCAs.
M.Sc.
2020-07-10 00:00:00
Advisors/Committee Members: Zhang, Xiao-an, Chemistry.
Subjects/Keywords: Contrast Agent Stability; Manganese Porphyrins; MRI Contrast Agents; Oxygen Sensor; Ratiometric MRI; 0574
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APA (6th Edition):
Tieu, H. (2018). Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/101584
Chicago Manual of Style (16th Edition):
Tieu, Henry. “Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents.” 2018. Masters Thesis, University of Toronto. Accessed January 16, 2021.
http://hdl.handle.net/1807/101584.
MLA Handbook (7th Edition):
Tieu, Henry. “Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents.” 2018. Web. 16 Jan 2021.
Vancouver:
Tieu H. Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1807/101584.
Council of Science Editors:
Tieu H. Redox Behaviour and Stability of Manganese Porphyrins Based MRI Contrast Agents. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/101584
University of Toronto
17.
Tabatabaeifar, Leila.
The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses.
Degree: 2013, University of Toronto
URL: http://hdl.handle.net/1807/35141
► Purpose: To compare hemodynamic of malignant and benign SRMs on CT and CEUS. Method: Seventy biopsy proven SRM underwent CEUS. Sixty-three had CT. After injection…
(more)
▼ Purpose: To compare hemodynamic of malignant and benign SRMs on CT and CEUS.
Method: Seventy biopsy proven SRM underwent CEUS. Sixty-three had CT. After injection of 0.2 ml of Definity, 3min and after 0.9 ml infusion, 30 sec of data were acquires. Lesion hemodynamics relative to the cortex was evaluated both qualitatively and quantitatively.
Results: Considering 15 and 20 HU as enhancement threshold, 10% to 13% of patients did not enhance on CT, while all lesions enhanced on CEUS. Papillary RCCs showed hypovascularity with 100% specificity. In other RCCs, PI, WI slope 5 to45%, 50 to100%, 10 to 90%, WO slope 100 to 50%, 100 to 10%, WO intensity at peak+30 seconds were statistically higher than benign SRMs.
Conclusion: All solid SRMs enhance on CEUS, while CT does not show vascularity in 10-13% of solid SRMs. CEUS can differentiate malignant from benign SRMs by evaluating their hemodynamics.
MAST
Advisors/Committee Members: Atri, Mostafa, Medical Science.
Subjects/Keywords: Contrast-enhanced ultrasound, microbubble contrast agent, solid small renal mass, kidney, cancer, Definity; 0574
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APA (6th Edition):
Tabatabaeifar, L. (2013). The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/35141
Chicago Manual of Style (16th Edition):
Tabatabaeifar, Leila. “The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses.” 2013. Masters Thesis, University of Toronto. Accessed January 16, 2021.
http://hdl.handle.net/1807/35141.
MLA Handbook (7th Edition):
Tabatabaeifar, Leila. “The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses.” 2013. Web. 16 Jan 2021.
Vancouver:
Tabatabaeifar L. The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1807/35141.
Council of Science Editors:
Tabatabaeifar L. The Utility of Contrast-enhanced Ultrasound in the Assessment of Solid Small Renal Masses. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/35141
Delft University of Technology
18.
Villamar Jorgge, J.A. (author).
Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage.
Degree: 2014, Delft University of Technology
URL: http://resolver.tudelft.nl/uuid:947d95ff-494f-482d-ad93-1cd1c63cd42c
► In the present study, the effect of concentration, osmolality and charge of x-ray contrast agents on their diffusion and equilibrium distribution across different zones of…
(more)
▼ In the present study, the effect of concentration, osmolality and charge of x-ray contrast agents on their diffusion and equilibrium distribution across different zones of cartilage was investigated. Full-thickness cartilage discs (Ø = 8.5 mm, n = 3) were extracted from healthy equine femoral condyle (n = 2). The diffusion of four different contrast agent baths (Condition A: Visipaque 320 mg/ml, 290 mOsmol/kg; Condition B: Visipaque 320 mg/ml, 600 mOsmol/kg; Condition C: Visipaque 160 mg/ml, 290 mOsmol/kg; Condition D: Hexabrix 320 mg/ml, 600 mOsmol/kg) was allowed only through the articular surface. Samples were imaged with a micro computed tomography scanner (micro-CT) before the contrast agent bath was applied, and after 5, 10, 20, 30 minutes and 1, 2, 3, 4, 5, 6, 7, 10, 12, 24, 30, 36, and 48 hours. Findings show that osmolality and concentration do not have a pronounced effect on diffusion. However, concentration influence on diffusion is seen on zonal curves. Moreover, the diffusion coefficient of Hexabrix was between 2.9 and 8.6 times lower than that of Visipaque that reflects the important effect of solute’s charge on the transport through charged hydrated tissue such as articular cartilage. Slightly different diffusion coefficient observed within dilute and concentrated Visipaque baths suggested deviation from ideal Fickean behavior within articular cartilage. However, close diffusion coefficients of cartilage exposed to low and high osmolality baths confirmed the minor effect of osmolality on the transport of neutral solutes.
BME
BioMechanical Engineering
Mechanical, Maritime and Materials Engineering
Advisors/Committee Members: Weinans, H.H. (mentor), Zadpoor, A.A. (mentor).
Subjects/Keywords: articular cartilage; diffusion; contrast agent; contrast enhanced computed tomography; Visipaque; Hexabrix; cartilage zones; diffusion coefficient
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APA (6th Edition):
Villamar Jorgge, J. A. (. (2014). Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage. (Masters Thesis). Delft University of Technology. Retrieved from http://resolver.tudelft.nl/uuid:947d95ff-494f-482d-ad93-1cd1c63cd42c
Chicago Manual of Style (16th Edition):
Villamar Jorgge, J A (author). “Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage.” 2014. Masters Thesis, Delft University of Technology. Accessed January 16, 2021.
http://resolver.tudelft.nl/uuid:947d95ff-494f-482d-ad93-1cd1c63cd42c.
MLA Handbook (7th Edition):
Villamar Jorgge, J A (author). “Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage.” 2014. Web. 16 Jan 2021.
Vancouver:
Villamar Jorgge JA(. Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage. [Internet] [Masters thesis]. Delft University of Technology; 2014. [cited 2021 Jan 16].
Available from: http://resolver.tudelft.nl/uuid:947d95ff-494f-482d-ad93-1cd1c63cd42c.
Council of Science Editors:
Villamar Jorgge JA(. Effect of Bath Condition on the Diffusion of Contrast Agents Across Articular Cartilage. [Masters Thesis]. Delft University of Technology; 2014. Available from: http://resolver.tudelft.nl/uuid:947d95ff-494f-482d-ad93-1cd1c63cd42c
NSYSU
19.
Liu, Jhih-Jhong.
Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning.
Degree: Master, Chemistry, 2007, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815107-212927
Subjects/Keywords: Contrast agent; Magic angle spinning
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APA (6th Edition):
Liu, J. (2007). Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815107-212927
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Liu, Jhih-Jhong. “Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning.” 2007. Thesis, NSYSU. Accessed January 16, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815107-212927.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Liu, Jhih-Jhong. “Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning.” 2007. Web. 16 Jan 2021.
Vancouver:
Liu J. Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning. [Internet] [Thesis]. NSYSU; 2007. [cited 2021 Jan 16].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815107-212927.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Liu J. Behavior of Magnetic Resonance Imaging Contrast Agents under Magic-Angle-Spinning. [Thesis]. NSYSU; 2007. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0815107-212927
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
20.
Hsin, Yu-Chun.
The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders.
Degree: Master, Chemistry, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526114-015528
► Owing to its many advantages such as noninvasiveness and its ability for providing multiple layers of information on structure and dynamics, magnetic resonance imaging (MRI)…
(more)
▼ Owing to its many advantages such as noninvasiveness and its ability for providing multiple layers of information on structure and dynamics, magnetic resonance imaging (MRI) has become an essential clinical equipment but also a powerful tool for basic research in various fields. By using MRI
contrast agents, more specificity and selectivity can be achieved with MRI, which has prompted the development of thousands of MRI
contrast agents over the past decades. The performance of the MRI
contrast agent depends on their chemical structure and the interaction with the other molecules around them such as water. The basic principle of MRI
contrast agents is to increase water relaxation with paramagnetic ions. The performance of an MRI
contrast agent depends not only on the structure and dynamics of the compound, but also on the environment it is located. In a typical living system, there are lots of macromolecules such as proteins, lipids, sugars, nucleic acids, ribosomes etc, making cell a highly crowded environment. To obtain insight into the influence of water motions by Dotarem under the crowded conditions, we use three types of crowding
agent (Ficoll 70ãNaPAãPEG6000). Ficoll 70 (70 kDa) is a synthetic crowding
agent and a cross-linked sucrose polymer. NaPA and PEG6000 are linear structural polymers.
We used nuclear magnetic resonance spectroscopy (NMR) to measure the longitudinal relaxation rate (R1), transverse relaxation rate (R2), translational diffusion, MRI and NMRD (Nuclear Magnetic Relaxation Dispersion) under different concentrations of crowders and
contrast agent. We have found that macromolecular crowding effect is an important contributing factor that affects the performance of MRI
contrast agents. The details of the crowding effect of different crowding agents are compared and discussed. The results demonstrate the significant effect of crowders on the relaxivity of MRI
contrast agent and indicate that a molecular level understanding of the relaxtion mechanism of MRI
contrast agent in a crowded environment helps establishment of a microscopic picture of MRI
contrast generation and prevents mistakes in diagnosis in medical MRI.
Advisors/Committee Members: Chun-hu Chen (chair), Shangwu (Sam) Ding (committee member), Jiin-Tsuey Cheng (chair), Chao-Ming Chiang (chair).
Subjects/Keywords: MRI; macromolecular; crowding effect; contrast agent; Ficoll 70
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APA (6th Edition):
Hsin, Y. (2014). The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526114-015528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hsin, Yu-Chun. “The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders.” 2014. Thesis, NSYSU. Accessed January 16, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526114-015528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hsin, Yu-Chun. “The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders.” 2014. Web. 16 Jan 2021.
Vancouver:
Hsin Y. The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 16].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526114-015528.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hsin Y. The Crowding Effect of Ficoll 70 on Magnetic Resonance Imaging Contrast Agent Dotarem and Comparison with Other Macromolecular Crowders. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526114-015528
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
21.
Oorschot, J.W.M. van.
Endogenous assessment of myocardial fibrosis with quantitative MRI.
Degree: 2016, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/328798
► In this thesis, we have studied the use of quantitative MR contrast mechanisms, that could potentially be used for endogenous detection of myocardial fibrosis in…
(more)
▼ In this thesis, we have studied the use of quantitative MR
contrast mechanisms, that could potentially be used for endogenous detection of myocardial fibrosis in the clinic. we have mainly focused on T2*, T1ρ MRI and Magnetization Transfer Imaging, and found that T1ρ -mapping, and Magnetization Transfer imaging are most promising. We found a significantly higher T1ρ-relaxation time in myocardial infarct tissue, compared to healthy remote tissue, both in a porcine animal model and chronic infarct patients. A comparison with LGE as gold standard for infarct detection, showed that native T1ρ –maps can be used to locate the myocardial scar area. For diffuse myocardial fibrosis, we have shown that T1ρ relaxation times correlate with the amount of fibrosis, determined by histology, and with extracellular volume fraction, measured in vivo. The results of native T1 –mapping in DCM patients did not indicate a significant relation with extracellular volume fraction in patients. Measurements of magnetization transfer in an animal model showed a decrease in magnetization transfer ratio in the infarct area, and the addition of an MT pulse in front of a T1ρ-mapping experiment resulted in a significantly higher T1ρ-relaxation time.
For infarct detection with T1ρ –mapping the main advantage compared to gold standard LGE imaging is that a
contrast agent is no longer required. As a first step towards clinical applications, this technique could be used as an alternative in patients that are unable to receive a gadolinium based
contrast agent, such as patients with severe renal failure. Also for diffuse fibrosis, T1ρ –mapping can be used as an alternative to the gadolinium based ECV-mapping. When the sensitivity of the technique is significantly increased, it could be considered as an alternative for LGE and ECV-mapping in clinical practice for all patients.
Advisors/Committee Members: Luijten, P.R., Leiner, T., Zwanenburg, J.J.M..
Subjects/Keywords: MRI; Cardiac; Heart; Endogenous; T1rho; Magnetization Transfer; Contrast agent; Native
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APA (6th Edition):
Oorschot, J. W. M. v. (2016). Endogenous assessment of myocardial fibrosis with quantitative MRI. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/328798
Chicago Manual of Style (16th Edition):
Oorschot, J W M van. “Endogenous assessment of myocardial fibrosis with quantitative MRI.” 2016. Doctoral Dissertation, Universiteit Utrecht. Accessed January 16, 2021.
http://dspace.library.uu.nl:8080/handle/1874/328798.
MLA Handbook (7th Edition):
Oorschot, J W M van. “Endogenous assessment of myocardial fibrosis with quantitative MRI.” 2016. Web. 16 Jan 2021.
Vancouver:
Oorschot JWMv. Endogenous assessment of myocardial fibrosis with quantitative MRI. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2016. [cited 2021 Jan 16].
Available from: http://dspace.library.uu.nl:8080/handle/1874/328798.
Council of Science Editors:
Oorschot JWMv. Endogenous assessment of myocardial fibrosis with quantitative MRI. [Doctoral Dissertation]. Universiteit Utrecht; 2016. Available from: http://dspace.library.uu.nl:8080/handle/1874/328798
University of California – Berkeley
22.
Ramirez, Richard Matthew.
Xenon Host-Guest Interactions for Applications in NMR and MRI.
Degree: Chemistry, 2013, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/5z2691dn
► The ability to detect the presence of specific analytes, whether in vivo or in heterogeneous solutions in vitro, is important for biomedical research as well…
(more)
▼ The ability to detect the presence of specific analytes, whether in vivo or in heterogeneous solutions in vitro, is important for biomedical research as well as chemical sensing devices. These applications demand sensitive detection in order to report on small quantities of the material of interest. For many diseases such as cancer, early detection of the important biomarkers when they exist at very low concentrations improves patient prognosis.NMR is noninvasive and does not require ionizing radiation, making it ideal for passive monitoring, and compatible with biological systems. It can be used to gain spectroscopic information, or alternatively to map the spatial distribution of a signal of interest, as is done with water protons in a 1H MRI experiment. The intrinsic sensitivity of NMR is low, however, due to low polarization which means that only ten protons out of a million are actually contributing to the observed signal. This is acceptable in an ordinary clinical MRI scan, where the in vivo concentration of protons is high ([H2O] $sim; 55 M), but severely limits its utility for low detection applications. Although contrast agents and methods have been successfully developed to address this problem they still lack the sensitivity to compete with nuclear medicine radioactive tracers which can be detected at sub-nanomolar concentrations—concentrations which better reflect the detection thresholds necessary for applications such as biomarker screening.This work considers the use of dissolved 129Xe gas as the imaging medium, rather than water protons. 129Xe can be “hyperpolarized” to increase its NMR signal by greater than 10,000-fold, resulting in signals comparable to that of water from considerably smaller concentrations (e.g. millimolar). Since xenon, a noble gas, cannot easily be functionalized for targeted applications, agents that interact with both xenon and a target are required. These hosts provide a unique environment for xenon such that a unique signature arises in the 129Xe NMR spectrum. This text describes the development, characterization, and application of xenon hosts with a sharp focus on creating new agents with improved sensitivity over state-of-the-art. Three different host platforms are presented. The first system builds upon a decade of previous work using the cage-like molecule cryptophane-A as the xenon host. Here, an agent composed of several hundred cryptophane-A molecules covalently attached to an M13 bacteriophage was synthesized and detected at sub-picomolar concentrations. The second system utilized emulsified, nanometer-sized perfluorocarbon-in-water droplets as the xenon host. This platform benefits from increased xenon payload and faster dynamics as opposed to cryptophane-based agents. Although a similar detection threshold was achieved for these nanoemulsion droplets, the time required for their detection was reduced by about 75%, and their…
Subjects/Keywords: Physical chemistry; contrast agent; hyperpolarized; molecular imaging; MRI; NMR; Xenon
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APA (6th Edition):
Ramirez, R. M. (2013). Xenon Host-Guest Interactions for Applications in NMR and MRI. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/5z2691dn
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ramirez, Richard Matthew. “Xenon Host-Guest Interactions for Applications in NMR and MRI.” 2013. Thesis, University of California – Berkeley. Accessed January 16, 2021.
http://www.escholarship.org/uc/item/5z2691dn.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ramirez, Richard Matthew. “Xenon Host-Guest Interactions for Applications in NMR and MRI.” 2013. Web. 16 Jan 2021.
Vancouver:
Ramirez RM. Xenon Host-Guest Interactions for Applications in NMR and MRI. [Internet] [Thesis]. University of California – Berkeley; 2013. [cited 2021 Jan 16].
Available from: http://www.escholarship.org/uc/item/5z2691dn.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ramirez RM. Xenon Host-Guest Interactions for Applications in NMR and MRI. [Thesis]. University of California – Berkeley; 2013. Available from: http://www.escholarship.org/uc/item/5z2691dn
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Neves, Herbert Rodrigo.
Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste.
Degree: Mestrado, Físico-Química, 2012, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-17042012-165853/
;
► Nanomateriais têm sido amplamente estudados, como resultado de suas propriedades físicas e químicas diferenciadas, que oferecem um grande número de possibilidades para aplicações em biomedicina,…
(more)
▼ Nanomateriais têm sido amplamente estudados, como resultado de suas propriedades físicas e químicas diferenciadas, que oferecem um grande número de possibilidades para aplicações em biomedicina, principalmente na terapia de câncer e no desenvolvimento de estratégias de diagnóstico não invasivo. O óxido de ferro superparamagnético (SPION) é o principal material estudado como agente de contraste para imagem por ressonância magnética, devido à sua capacidade de reduzir o tempo de relaxação transversal (T2) em diferentes tecidos e sua menor toxicidade que os complexos de Gd3+ e Mn2+ usados atualmente. Entretanto, o acumulo de SPIONs pode ser facilmente confundido com sinais referentes à calcificação, depósito de metais pesados e sangramentos, e a alta susceptibilidade magnética do material promove distorções na imagem. Assim, alguns aspectos são desejáveis em material para que este tenha potencial para substituir o SPION, tais como forma nanoparticulada, para fácil modificação de superfície e possibilidade de funcionalização com agentes biosseletivos, e contraste positivo em T1. As nanopartículas (NPs) antiferromagnéticas de MnO atendem a todos os requisitos necessários para substituir o óxido de ferro. As NPs de MnO foram sintetizadas a partir da decomposição térmica do acetilacetonato de manganês(II) em uma variação do método poliol modificado, resultando na formação de NPs com tamanho médio de 21 ± 3,9 nm. Foi realizada a substituição de ligantes de superfície para que se substituísse o ácido oleico adsorvido sobre o material por 3-aminopropiltrimetoxisilano (APTMS) e foi determinada a concentração de grupamentos amino sobre a superfície das NPs. Posteriormente, obteve-se uma estrutura do tipo \"core/shelld̈ispersível em meio aquoso e biocompatível pela reação dos grupos amino livres com o carboxilato da carboximetil dextrana (CMDex). O potencial de superfície e a estabilidade coloidal das NPs funcionalizadas foram caracterizados por mobilidade eletroforética e por espalhamento de luz dinâmico em água deionizada e em condições que mimetizavam o sangue. As NPs apresentaram toxicidade em células cancerosas de carcinoma cervical humano (HeLa). Entretanto, não foi observada toxicidade significativa na linhagem de células não cancerosas NCTC clone L929. Tanto as NPs como sintetizadas quanto as recobertas com CMDex apresentaram controle de tamanho e forma, apresentando distribuição de tamanho compatível com o esperado para as aplicações em biomedicina.
Nanomaterials have been widely studied as a result of their interesting physical and chemical properties, which offer a large number of possibilities for applications in biomedicine mainly in cancer therapy and the development of strategies for non-invasive diagnosis. The superparamagnetic iron oxide nanoparticles (SPION) is the main studied material as contrast agent for magnetic resonance imaging (MRI) due to its ability to reduce the transverse relaxation time (T2) in different tissues and lower toxicity than Gd3+ and Mn2+ complexes currently used. However, this…
Advisors/Committee Members: Varanda, Laudemir Carlos.
Subjects/Keywords: agente de contraste; antiferromagnetism; antiferromagnetismo; contrast agent; MnO; MnO
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APA (6th Edition):
Neves, H. R. (2012). Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/75/75134/tde-17042012-165853/ ;
Chicago Manual of Style (16th Edition):
Neves, Herbert Rodrigo. “Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste.” 2012. Masters Thesis, University of São Paulo. Accessed January 16, 2021.
http://www.teses.usp.br/teses/disponiveis/75/75134/tde-17042012-165853/ ;.
MLA Handbook (7th Edition):
Neves, Herbert Rodrigo. “Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste.” 2012. Web. 16 Jan 2021.
Vancouver:
Neves HR. Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste. [Internet] [Masters thesis]. University of São Paulo; 2012. [cited 2021 Jan 16].
Available from: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-17042012-165853/ ;.
Council of Science Editors:
Neves HR. Nanopartículas antiferromagnéticas de MnO para aplicações em biomedicina como agentes de contraste. [Masters Thesis]. University of São Paulo; 2012. Available from: http://www.teses.usp.br/teses/disponiveis/75/75134/tde-17042012-165853/ ;
Vanderbilt University
24.
Coffey, Aaron Michael.
Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields.
Degree: PhD, Biomedical Engineering, 2014, Vanderbilt University
URL: http://hdl.handle.net/1803/12515
► Nuclear spin polarization P is a key factor in overall Magnetic Resonance (MR) sensitivity, and conventionally is of order 10-6 owing to the tyranny of…
(more)
▼ Nuclear spin polarization
P is a key factor in overall Magnetic Resonance (MR) sensitivity, and conventionally is of order 10
-6 owing to the tyranny of its induction by the strength of the detection magnetic field. But various hyperpolarization mechanisms applied externally to the detection field can temporarily increase nuclear spin polarization to near unity (
P = 1). The resulting increased MR signal enables a variety of applications, including biomedical use of hyperpolarized (HP)
contrast agents to assay cellular metabolism via Magnetic Resonance Imaging (MRI), typically
13C-labeled metabolites reporting on abnormal metabolism. In this work optimization of radiofrequency (RF) coils and hyperpolarizer automation are used to increase the detection sensitivity of hyperpolarized
contrast agents (HCA) and improve their production. It is shown that low-field imaging can be more sensitive than corresponding high-field detection when using RF coils optimized to the resonant frequency. The feasibility of low-field molecular imaging of
1H and
13C HCA with high spatial resolution (as fine as 94×94 μm
2) is demonstrated with low-field 38 mm inner diameter RF coils at a static magnetic field strength
B0 = 0.0475 T, achieving signal-to-noise ratios suitable for
in vivo imaging studies.
Advisors/Committee Members: Kevin W. Waddell (committee member), John C. Gore (committee member), William A. Grissom (committee member), Wellington Pham (committee member), Eduard Y. Chekmenev (Committee Chair).
Subjects/Keywords: parahydrogen; xenon-129; low-field MRI; hyperpolarization; hyperpolarizer; contrast agent
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Manager
APA (6th Edition):
Coffey, A. M. (2014). Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12515
Chicago Manual of Style (16th Edition):
Coffey, Aaron Michael. “Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields.” 2014. Doctoral Dissertation, Vanderbilt University. Accessed January 16, 2021.
http://hdl.handle.net/1803/12515.
MLA Handbook (7th Edition):
Coffey, Aaron Michael. “Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields.” 2014. Web. 16 Jan 2021.
Vancouver:
Coffey AM. Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields. [Internet] [Doctoral dissertation]. Vanderbilt University; 2014. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1803/12515.
Council of Science Editors:
Coffey AM. Sensitive Molecular Magnetic Resonance Imaging Of Hyperpolarized Contrast Agents In Low Magnetic Fields. [Doctoral Dissertation]. Vanderbilt University; 2014. Available from: http://hdl.handle.net/1803/12515
University of Toronto
25.
Hindy, Salma.
Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer.
Degree: 2018, University of Toronto
URL: http://hdl.handle.net/1807/89629
► This thesis explores CF800, a multimodal nanoliposomal contrast agent that co-encapsulates a near infrared agent indocyanine green, and a computed tomography (CT) agent iohexol in…
(more)
▼ This thesis explores CF800, a multimodal nanoliposomal contrast agent that co-encapsulates a near infrared agent indocyanine green, and a computed tomography (CT) agent iohexol in pre-clinical human lung cancer models for intraoperative visualization. Pre-clinical models rely heavily on a basement membrane extract, Matrigel, for the localization and formation of orthotopic lung xenografts. Matrigel has been suspected of altering pre-clinical models with a negative affect on the accumulation of nanoparticles. Mice were injected with human lung adenocarcinoma cancer cells with 0%, 50% and 100% Matrigel concentrations. Mice were injected with CF800 after tumor formation and optical images were acquired. CF800 significantly increased the visual fluorescence of the tumors but not CT enhancement. Xenografts with Matrigel exhibited lower fluorescence and CF800 accumulation, although not significantly. The tumor rate of growth and size had a significant effect on nanoparticle accumulation, showing that factors other than Matrigel affect visualization and accumulation of CF800 intraoperatively.
M.H.Sc.
Advisors/Committee Members: Yasufuku, Kazuhiro, Biomedical Engineering.
Subjects/Keywords: ICG; lung cancer; nanoparticles; NIR contrast agent; pre-clinical model; 0541
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APA (6th Edition):
Hindy, S. (2018). Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/89629
Chicago Manual of Style (16th Edition):
Hindy, Salma. “Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer.” 2018. Masters Thesis, University of Toronto. Accessed January 16, 2021.
http://hdl.handle.net/1807/89629.
MLA Handbook (7th Edition):
Hindy, Salma. “Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer.” 2018. Web. 16 Jan 2021.
Vancouver:
Hindy S. Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1807/89629.
Council of Science Editors:
Hindy S. Evaluating the accumulation of dual-modal indocyanine green-containing liposomes in pre-clinical models for visualizing human lung cancer. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/89629
University of Arizona
26.
Kombala Nanayakkara Thambiliyagodage, Chathuri Jeewanthi.
Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
.
Degree: 2020, University of Arizona
URL: http://hdl.handle.net/10150/642025
► The extracellular tumor microenvironments of many solid tumors have high acidosis and high protease activity. The detection of tumor acidosis and protease activity can potentially…
(more)
▼ The extracellular tumor microenvironments of many solid tumors have high acidosis and high protease activity. The detection of tumor acidosis and protease activity can potentially impact the cancer diagnosis. Noninvasive imaging methods have previously been developed that measure extracellular pH or can detect enzyme activity using Chemical Exchange Saturation Transfer Magnetic Resonance Imaging (CEST MRI). CEST MRI is an inherently insensitive MRI modality which requires a high concentration of small molecule
agent to be delivered to the tumor. Therefore, nanosized molecules have received increased attention to be used in CEST imaging to improve the sensitivity.
Herein, we developed a nanoscale CEST
agent that can measure pH using acidoCEST MRI, which may decrease the requirement for high delivery concentrations of
agent. We also developed a monomer
agent for comparison to the polymer. After optimizing CEST experimental conditions, we determined that the polymer
agent could be used during acidoCEST MRI studies at 125-fold and 488-fold lower concentration than the monomer
agent and iopamidol, respectively. In vivo acidoCEST MRI studies using the three agents were performed to study a xenograft MDA-MB-231 model of mammary carcinoma. The tumor pHe measurements were 6.33 ± 0.12, 6.70 ± 0.15, and 6.85 ± 0.15 units with iopamidol, the monomer
agent and polymer
agent, respectively. The higher pHe measurements with the new agents was attributed to the concentration dependence of these agents. This study demonstrated that nanoscale agents have merit for CEST MRI studies, but consideration should be given to the dependence of CEST
contrast on the concentration of these agents.
We also investigated the development and application of a single hybrid CEST
agent that can simultaneously measure pH and evaluate protease activity using a combination of dual-power acidoCEST MRI and catalyCEST MRI. Simultaneously assessing both characteristics may improve diagnostic evaluations of aggressive tumors and the effects of anti-cancer treatments. Our
agent showed CEST signals at 9.2 ppm from a salicylic acid moiety and at 5.0 ppm from an aryl amide. The CEST signal at 9.2 ppm could be measured after selective saturation was applied at 1 and 4 T, and these measurements could be used with a ratiometric analysis to determine pH. The CEST signal at 5.0 ppm from the aryl amide disappeared after the
agent was treated with cathepsin-B, while the CEST signal at 9.2 ppm remained, indicating that the
agent could detect protease activity through amide bond clevage. Michaelis-Menton kinetics studies with catalyCEST MRI demonstrated that the binding affinity (as shown with the Michaelis constant KM), the catalytic turnover rate (kcat), and catalytic efficiency (kcat/kM), were each higher for cathepsin B at lower pH. The kcat rates measured with catalyCEST MRI were lower than the comparable rates measured with LC-MS, which reflected a limitation of inherently noisy and relatively insensitive CEST MRI analyses. Although this level of precision limited…
Advisors/Committee Members: Mash, Eugene A (advisor), Pagel, Mark D. (committeemember), Charest, Pascale G. (committeemember), Kuo, Philip H. (committeemember).
Subjects/Keywords: CEST;
Contrast agent;
Enzyme activity;
Magnetic resonance imaging;
pH;
Polymer
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Kombala Nanayakkara Thambiliyagodage, C. J. (2020). Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
. (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/642025
Chicago Manual of Style (16th Edition):
Kombala Nanayakkara Thambiliyagodage, Chathuri Jeewanthi. “Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
.” 2020. Doctoral Dissertation, University of Arizona. Accessed January 16, 2021.
http://hdl.handle.net/10150/642025.
MLA Handbook (7th Edition):
Kombala Nanayakkara Thambiliyagodage, Chathuri Jeewanthi. “Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
.” 2020. Web. 16 Jan 2021.
Vancouver:
Kombala Nanayakkara Thambiliyagodage CJ. Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
. [Internet] [Doctoral dissertation]. University of Arizona; 2020. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10150/642025.
Council of Science Editors:
Kombala Nanayakkara Thambiliyagodage CJ. Development and Application of CEST MRI Contrast Agents that Evaluate Tumor Acidosis and Enzyme Activity
. [Doctoral Dissertation]. University of Arizona; 2020. Available from: http://hdl.handle.net/10150/642025
Wayne State University
27.
Vithanarachchi, Sashiprabha Manjari.
Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging.
Degree: PhD, Chemistry, 2014, Wayne State University
URL: https://digitalcommons.wayne.edu/oa_dissertations/932
► The focus of the research described in this thesis is the study of chemistry relevant to target-specific contrast agents for magnetic resonance imaging (MRI).…
(more)
▼ The focus of the research described in this thesis is the study of chemistry relevant to target-specific
contrast agents for magnetic resonance imaging (MRI). MRI is a widely used technique in diagnostic medicine and biomedical research to obtain anatomical and physiological details of soft tissues.
Contrast agents are used to enhance the
contrast of MR images by causing changes to the chemical environment of water molecules. Clinically approved GdIII-containing
contrast agents for MRI are non-specific, and consequently, have limited utility. Target-specific
contrast agents represent one way to circumvent this limitation. In the research described in this thesis, myelin and Β-amyloid aggregates were selected as targets because they are important in diagnosing neurological diseases. The myelin-targeted complexes were designed to mimic the structural features of a known myelin-specific histology stain, and ex vivo mouse-brain staining method was developed to test these complexes. Ex vivo staining studies (optical, MRI, and mass spectrometry imaging) demonstrated the ability of these complexes to interact with the myelinated regions in mouse brain tissue. Additionally, a Β-amyloid-targeted
agent was synthesized by conjugating a GdIII-containing complex to curcumin. The binding ability of this complex with in vitro Β-amyloid peptide aggregates was studied using relaxation time and fluorescence measurements. This dissertation presents the synthesis, characterization, and in vitro and ex vivo imaging of these complexes. The studies using these paramagnetic metal complexes have the potential to enable a reliable method to observe structural changes in the brain.
Advisors/Committee Members: Matthew J. Allen.
Subjects/Keywords: beta-amyloid; contrast agent; gadolinium; Magnetic resonance imaging; multimodal; myelin; Chemistry
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APA (6th Edition):
Vithanarachchi, S. M. (2014). Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/932
Chicago Manual of Style (16th Edition):
Vithanarachchi, Sashiprabha Manjari. “Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging.” 2014. Doctoral Dissertation, Wayne State University. Accessed January 16, 2021.
https://digitalcommons.wayne.edu/oa_dissertations/932.
MLA Handbook (7th Edition):
Vithanarachchi, Sashiprabha Manjari. “Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging.” 2014. Web. 16 Jan 2021.
Vancouver:
Vithanarachchi SM. Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging. [Internet] [Doctoral dissertation]. Wayne State University; 2014. [cited 2021 Jan 16].
Available from: https://digitalcommons.wayne.edu/oa_dissertations/932.
Council of Science Editors:
Vithanarachchi SM. Synthesis And Characterization Of New Gd3+-Containing Complexes As Potential Targeted Contrast Agents For Magnetic Resonance Imaging. [Doctoral Dissertation]. Wayne State University; 2014. Available from: https://digitalcommons.wayne.edu/oa_dissertations/932
28.
松岡, 英太郎.
マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究.
Degree: 修士(環境学), 2017, The University of Tokyo / 東京大学
URL: http://hdl.handle.net/2261/37202
► A new fabrication method of hollow poly (allylamine hydrochloride)/poly (sodium 4-styrenesulfonate) (PAH/PSS) microcapsules was proposed using microbubble templates without surfactants. In a Na2CO3 aqueous solution,…
(more)
▼ A new fabrication method of hollow poly (allylamine hydrochloride)/poly (sodium 4-styrenesulfonate) (PAH/PSS) microcapsules was proposed using microbubble templates without surfactants. In a Na2CO3 aqueous solution, PAH becomes colloidal particles of R-NHCOO- and R-NH3 + within a certain pH range. Under such a condition, if microbubbles are present in the solution, the colloidal particles adsorb to the microbubble surface, stabilizing the microbubbles. When PSS is added to the solution sequentially, a bilayer of PAH/PSS can be formed around microbubbles. We successfully fabricated hollow PAH/PSS microcapsules by this method and elucidated the conditions required for the formation of colloidal particles of PAH and a bilayer of PAH/PSS. The size of microcapsules was also successfully controlled by changing the concentration of PAH.
Subjects/Keywords: Hollow microcapsule; Microbubble; Template technique; Biodegradable polymer; Ultrasound contrast agent
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APA (6th Edition):
松岡, . (2017). マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究. (Thesis). The University of Tokyo / 東京大学. Retrieved from http://hdl.handle.net/2261/37202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
松岡, 英太郎. “マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究.” 2017. Thesis, The University of Tokyo / 東京大学. Accessed January 16, 2021.
http://hdl.handle.net/2261/37202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
松岡, 英太郎. “マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究.” 2017. Web. 16 Jan 2021.
Vancouver:
松岡 . マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究. [Internet] [Thesis]. The University of Tokyo / 東京大学; 2017. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/2261/37202.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
松岡 . マイクロバブルを利用した中空高分子電解質マイクロカプセル製造に関する研究. [Thesis]. The University of Tokyo / 東京大学; 2017. Available from: http://hdl.handle.net/2261/37202
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
29.
Weber, Judith.
Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress.
Degree: PhD, 2019, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/294385
► An early and precise diagnosis of disease is a crucial requirement for fast and targeted therapy in the era of precision medicine. Most diseases possess…
(more)
▼ An early and precise diagnosis of disease is a crucial requirement for fast and targeted therapy in the era of precision medicine. Most diseases possess molecular alterations in their early stages, which precede noticeable morphological changes. One important molecular change that contributes to dysfunction in a wide range of pathologies, including cancer and neurological disorders, is an increase in levels of reactive oxygen species (ROS) and associated oxidative damage. Our understanding of how ROS influence disease biology is currently limited by our inability to perform sensitive and specific assessment of ROS levels with high spatial and temporal resolution in living systems.
The goal of the research described in this thesis was to overcome the challenge of assessing ROS during disease development in cancer and neurodegenerative disease through the design, synthesis and validation of two classes of novel bifunctional, ROS-sensitive contrast agents.
To shed light on the complex redox biology in cancer, the new method of photoacoustic imaging was exploited. A novel activatable, targeted near infrared cyanine dye is reported that enables specific detection of pathological levels of hydrogen peroxide, a major and abundant ROS in living organisms. This approach uses photoacoustic and fluorescence imaging in cancerous tissue to evaluate the performance of the new probe under in vitro and in vivo conditions.
In neurodegeneration, there exists a bidirectional interaction between oxidative stress and protein aggregates. To scrutinise this relationship, both bulk and single-molecule fluorescence imaging methods were used to assess the capability of novel bifunctional fluorescence dyes to localise the presence of the two putative disease-causing species, ROS and protein aggregates, simultaneously under in vitro conditions.
The data shown here provides a promising foundation for the systematic design of contrast agents based on small molecule dyes, that possess ideal optical and biological characteristics to study oxidative stress in a broad range of pathological applications with high temporal and spatial resolution.
Subjects/Keywords: contrast agent; oxidative stress; photoacoustic imaging; fluorescence imaging/microscopy; amyloid aggregates
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Weber, J. (2019). Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/294385
Chicago Manual of Style (16th Edition):
Weber, Judith. “Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress.” 2019. Doctoral Dissertation, University of Cambridge. Accessed January 16, 2021.
https://www.repository.cam.ac.uk/handle/1810/294385.
MLA Handbook (7th Edition):
Weber, Judith. “Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress.” 2019. Web. 16 Jan 2021.
Vancouver:
Weber J. Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress. [Internet] [Doctoral dissertation]. University of Cambridge; 2019. [cited 2021 Jan 16].
Available from: https://www.repository.cam.ac.uk/handle/1810/294385.
Council of Science Editors:
Weber J. Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress. [Doctoral Dissertation]. University of Cambridge; 2019. Available from: https://www.repository.cam.ac.uk/handle/1810/294385
University of Arizona
30.
Goldsher, Anetta Victoria.
Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
.
Degree: 2017, University of Arizona
URL: http://hdl.handle.net/10150/625887
► Detection of enzyme activity has gained popularity in molecular imaging because increased activity of enzymes such as urokinase plasminogen activator (uPA) can serve as biomarkers…
(more)
▼ Detection of enzyme activity has gained popularity in molecular imaging because increased activity of enzymes such as urokinase plasminogen activator (uPA) can serve as biomarkers and assist in cancer diagnosis. Chemical exchange saturation transfer (CEST) Magnetic Resonance Imaging (MRI) is a non-invasive technique that can be utilized to detect enzyme activity; however, CEST MRI is not the only technique that can assess enzyme activity. Chapter 1 provides an overview of various imaging modalities that have been used to detect enzyme activity in vivo. Advances made in probe-design are discussed, in addition to advantages and disadvantages of each technique. Chapter 2 focuses on detection of uPA activity in a pancreatic cancer tumor model using a catalyCEST MRI
contrast agent. Chapter 2 also discusses the importance of uPA in tumor biology, addresses the synthesis of the
contrast agent, and evaluates the results of in vivo detection and ex vivo validation of uPA activity in response to therapy of pancreatic tumor models of Capan-2. The in vivo and ex vivo results showed no significant difference in uPA activity between chemotherapy-treated and non-treated mice. Additionally, no significant difference was observed between before and after chemotherapy-treated groups. Chapter 3 addresses some of the limitations of the study detailed in Chapter 2 and proposes improvements.
Advisors/Committee Members: Pagel, Mark D (advisor), Jewett, John C (advisor), Pagel, Mark D. (committeemember), Jewett, John C. (committeemember), Baker, Amanda F. (committeemember).
Subjects/Keywords: CEST;
contrast agent;
molecular imaging;
MRI;
pancretic cancer;
uPA
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Goldsher, A. V. (2017). Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
. (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/625887
Chicago Manual of Style (16th Edition):
Goldsher, Anetta Victoria. “Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
.” 2017. Masters Thesis, University of Arizona. Accessed January 16, 2021.
http://hdl.handle.net/10150/625887.
MLA Handbook (7th Edition):
Goldsher, Anetta Victoria. “Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
.” 2017. Web. 16 Jan 2021.
Vancouver:
Goldsher AV. Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
. [Internet] [Masters thesis]. University of Arizona; 2017. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10150/625887.
Council of Science Editors:
Goldsher AV. Detection of Enzyme Activity in a Pancreatic Tumor Model Using CatalyCEST Contrast MRI
. [Masters Thesis]. University of Arizona; 2017. Available from: http://hdl.handle.net/10150/625887
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Open Access Theses and Dissertations
