Open Access Theses and Dissertations

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You searched for subject:(collagen fragments). Showing records 1 – 2 of 2 total matches.

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Penn State University

1. Chin-quee, Karis P. Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look .

Degree: 2013, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/17496 

ABSTRACT Successful metastasis requires some degree of hospitality from the host organ that is metastasized. In recent years, evidence has been mounting that the primary tumor contributes to adapting this remote organ to increase this hospitality. Interestingly, many in vitro experiments have shown that conditioned media from cancer cells produce physical changes which theoretically could facilitate invasion. These include perturbations in extracellular matrix and of cell to cell adhesions. The conditioned media in these in vitro experiments could be interpreted to be a proxy for either paracrine or endocrine effects, however, when these observations are coupled with evidence from in vivo experiments, it seems possible that primary tumors have remote effects on un-metastasized organs that can elicit changes in them which are pro-metastatic. We have performed a series of experiments designed to characterize and describe changes in osteoblasts (hFOB) which occur as a result of exposure to conditioned medium from breast cancer cells (MDA-MET). Functional experiments aimed at investigating whether these changes are pro-metastatic were included as a means of this characterization. Thus cell to cell adhesions, as well as the ability of the osteoblastic environment to attract MDA-MET cells were both assessed. Further, we investigated mechanistically the bases behind the observations from the functional experiments. Methods Confluent hFOB cells were treated with conditioned media from either MDA-MET, MDA-MB-231 HTERT-HME1 or hFOB cells 24 hrs. This treatment conditioned medium was removed, the cells washed and serum-free medium added to the hFOB cells. hFOB-conditioned medium was collected after 18hrs. This medium was then used in in vitro migration assays measuring number of MDA-MET migratory cells by labeling the cells with DNA-binding dye Cyquant; in establishing presence of collagen by western blot; in collagenase assays and in a cytokine array where antibodies to 74 cytokines were embedded on a membrane. The presence of Type 1 collagen receptors was shown by immunocytochemistry. The data were analyzed using one way ANOVA and the Student’s T test. Results We found that conditioned medium from hFOB cells that had been treated with MDA-MET-conditioned medium attracted more MDA-MET cells than hFOB cells pre-exposed to its own medium only. We hypothesized that Type 1 collagen fragments of specific length were the chemoattractants responsible for our observed effect. This was evidenced by an increase in collagenase in the conditioned medium of hFOB cells which had been exposed to MDA-MET-conditioned medium. The definitiveness of this evidence was aided by the inherent fidelity of the collagenase enzymes and its Type 1 collagen substrate and the distinctiveness of the product of this enzyme substrate interaction. We also showed that bacterial collagenase, which creates short collagen fragments of non-specific length removed the ability of hFOB -conditioned medium to attract MDA-MET cells. In addition, we showed that at… Advisors/Committee Members: Henry Joseph Donahue, Dissertation Advisor/Co-Advisor, Henry Joseph Donahue, Committee Chair/Co-Chair, Andrea Manni, Committee Member, Andrea Marie Mastro, Committee Member, David Feith, Committee Member, Lisa M Shantz, Committee Member, Ralph Lauren Keil, Committee Member.

Subjects/Keywords: breast cancer metastasis; pre-metastatic niche; bone; collagen fragments; collagenase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Chin-quee, K. P. (2013). Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look . (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/17496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Chin-quee, Karis P. “Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look .” 2013. Thesis, Penn State University. Accessed March 08, 2021. https://submit-etda.libraries.psu.edu/catalog/17496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Chin-quee, Karis P. “Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look .” 2013. Web. 08 Mar 2021.

Vancouver:

Chin-quee KP. Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look . [Internet] [Thesis]. Penn State University; 2013. [cited 2021 Mar 08]. Available from: https://submit-etda.libraries.psu.edu/catalog/17496.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chin-quee KP. Breast cancer cell secretions promote a pre-metastatic niche in bone; an in vitro look . [Thesis]. Penn State University; 2013. Available from: https://submit-etda.libraries.psu.edu/catalog/17496

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Bann, James Gerald. Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix.

Degree: PhD, 2000, Oregon Health Sciences University

URL: doi:10.6083/M4SJ1HX5  ; http://digitalcommons.ohsu.edu/etd/3323 

Subjects/Keywords: Peptide Fragments; Collagen; Glycosylation; Polychaeta; Molecular Sequence Data; Galactose; Threonine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bann, J. G. (2000). Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4SJ1HX5 ; http://digitalcommons.ohsu.edu/etd/3323

Chicago Manual of Style (16th Edition):

Bann, James Gerald. “Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix.” 2000. Doctoral Dissertation, Oregon Health Sciences University. Accessed March 08, 2021. doi:10.6083/M4SJ1HX5 ; http://digitalcommons.ohsu.edu/etd/3323.

MLA Handbook (7th Edition):

Bann, James Gerald. “Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix.” 2000. Web. 08 Mar 2021.

Vancouver:

Bann JG. Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 2000. [cited 2021 Mar 08]. Available from: doi:10.6083/M4SJ1HX5 ; http://digitalcommons.ohsu.edu/etd/3323.

Council of Science Editors:

Bann JG. Synthesis and biophysical characterization of collagen-like peptides : glycosylation-induced stabilization of the triple-helix. [Doctoral Dissertation]. Oregon Health Sciences University; 2000. Available from: doi:10.6083/M4SJ1HX5 ; http://digitalcommons.ohsu.edu/etd/3323

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