subject:(choroid plexus)

Tulane University

1. Delery, Elizabeth. Choroid Plexus in AIDS Pathogenesis.

Degree: 2019, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:94533

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The prevalence of HIV-associated neurocognitive disorders (HAND) has increased in the era of combination anti-retroviral therapy (cART). Despite this and documented neurocognitive impairment, there… (more)

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arch[email protected]

The prevalence of HIV-associated neurocognitive disorders (HAND) has increased in the era of combination anti-retroviral therapy (cART). Despite this and documented neurocognitive impairment, there is a lack of pathology of HIV-encephalitis (HIVE), specifically multi-nucleated giant cells (MNGCs), in children and SIV-encephalitis (SIVE) in rhesus macaques infected pre-, peri-, and post-parturition. In this dissertation, we show that the lack of MNGCs seen is most likely due to innate differences in the blood-brain and blood-CSF barriers, and a robust pro- and anti-inflammatory response in neonatal rhesus macaques. Using a rhesus macaque model of HIV, we examined the plasma viral load, brain tissue viral load, and monocyte turnover, using PCR and flow cytometry, respectively. We also performed immunohistochemistry for monocyte, macrophage, tight junction, and aging markers of the choroid plexus. We sought to create a choroid plexus epithelial cell model to monitor the effects of inflammatory markers and virus on the tight junctions of the blood-CSF barrier in real-time. We demonstrated that neonates do not develop encephalitis, despite comparable viral load and monocyte turnover, previously established correlates of SIV-encephalitis (SIVE). However, we noted that uninfected adult rhesus macaques have an increase in virus susceptible cells in the brain, SIV-infected adults have a leakier blood-brain barrier than infected neonates, and adults with encephalitis have a greater viral burden in brain tissue compared to adults without encephalitis. In the choroid plexus, we discovered that despite the lack of encephalitis, neonates have an increase in monocytes and macrophages of the choroid plexus, indicating a strong immune response. While our choroid plexus epithelial cell model is still in preliminary stages, initial results are promising. Our work indicates a possible viral threshold needed for the development of encephalitis, and that the blood-brain barrier may play a role in this threshold due to lower levels of virus susceptible cells and a tighter blood brain barrier in neonates. In the choroid plexus, the strong pro- and anti-inflammatory macrophage response seen in neonates may offer an extra layer of protection development of SIVE. Our data also indicates that SIV causes a marked decrease in the expression of klotho, the anti-aging hormone that is produced in high levels in the choroid plexus in the brain. This could potentially explain the premature inflammaging phenotype seen in chronic infections.

1

Elizabeth Delery

Advisors/Committee Members: MacLean, Andrew (Thesis advisor), School of Medicine Biomedical Sciences Graduate Program (Degree granting institution).

Subjects/Keywords: Choroid Plexus; HIV; Macrophages

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APA (6^th Edition):

Delery, E. (2019). Choroid Plexus in AIDS Pathogenesis. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:94533

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Delery, Elizabeth. “Choroid Plexus in AIDS Pathogenesis.” 2019. Thesis, Tulane University. Accessed March 07, 2021. https://digitallibrary.tulane.edu/islandora/object/tulane:94533.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Delery, Elizabeth. “Choroid Plexus in AIDS Pathogenesis.” 2019. Web. 07 Mar 2021.

Vancouver:

Delery E. Choroid Plexus in AIDS Pathogenesis. [Internet] [Thesis]. Tulane University; 2019. [cited 2021 Mar 07]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:94533.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Delery E. Choroid Plexus in AIDS Pathogenesis. [Thesis]. Tulane University; 2019. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:94533

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

2. Dropp, John Jerome. The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus).

Degree: PhD, Zoology, 1969, Oregon State University

URL: http://hdl.handle.net/1957/46637

► The patterns of cell proliferation in the choroid plexuses of embryonic and post-natal hamsters were studied by means of auto-radiography (H³-thymidine). In addition, the PAS… (more)

Subjects/Keywords: Choroid plexus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dropp, J. J. (1969). The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46637

Chicago Manual of Style (16^th Edition):

Dropp, John Jerome. “The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus).” 1969. Doctoral Dissertation, Oregon State University. Accessed March 07, 2021. http://hdl.handle.net/1957/46637.

MLA Handbook (7^th Edition):

Dropp, John Jerome. “The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus).” 1969. Web. 07 Mar 2021.

Vancouver:

Dropp JJ. The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus). [Internet] [Doctoral dissertation]. Oregon State University; 1969. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1957/46637.

Council of Science Editors:

Dropp JJ. The histogenesis and mode of growth of the choroid plexuses of the hamster (Mesocricetus auratus). [Doctoral Dissertation]. Oregon State University; 1969. Available from: http://hdl.handle.net/1957/46637

University of Illinois – Chicago

3. Muniz-Talavera, Hilmarie. Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus.

Degree: 2016, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/20870

► The cerebrospinal fluid (CSF) is produced by the choroid plexus and is contained within the brain ventricular system (Damkier et al., 2013). CSF flows from… (more)

▼ The cerebrospinal fluid (CSF) is produced by the choroid plexus and is contained within the brain ventricular system (Damkier et al., 2013). CSF flows from the lateral ventricles to the third ventricle, where it then enters the fourth ventricle through the cerebral aqueduct (Brinker et al., 2014). The composition of the CSF is derived from passive filtration of plasma and membrane secretion and it has three mains functions: 1) protect the brain by serving as a cushion against mechanical shock, 2) serve as a route for nutrient delivery and signaling factors, and 3) carry waste products and toxins away from the brain (Lun et al., 2015; Spector et al., 2015). CSF production and circulation should be carefully regulated to allow proper brain development (Gato et al., 2014; Mohammad Nabiuni, 2015). Increased CSF volume causes ventricular dilation and it can lead to the development of a neurological condition known as hydrocephalus. Congenital hydrocephalus is the most common form of the disease, which is present at birth and it affects 1-2/1000 children in the United States (Kahle et al., 2015). Hydrocephalus can result from abnormalities in the production, flow or absorption of the CSF, and if untreated it can lead to death. The ventricles of the brain are lined by a monolayer of ciliated squamous epithelia known as the ependymal cells. The ependyma carry motile cilia (9+2), which coordinately beat to produce a laminar flow and promote CSF circulation throughout the ventricles (Spassky, 2013). The choroid plexus is composed of a monolayer of modified ependymal cells though these cells have developed a unique polarization of membrane associated proteins, along with other secretory properties that allow choroid plexus (CP) cells to function in CSF secretion (Damkier et al., 2013; Dziegielewska et al., 2001). Hydrocephalus can result from the disruption of ependymal cells and/or the choroid plexus by the loss of CSF flow and CSF overproduction, respectively (Baas et al., 2006a; Banizs et al., 2005). Despite the undeniable importance of the ependyma and the CP in CSF homeostasis, the mechanisms and factors involved in their differentiation are largely unknown. The work presented here aimed to further characterize the structure and function of the ependymal cells and the specialized ependymal cells of the CP. The JhylacZ mouse line carries an insertional mutation in the Jhy gene (formerly 4931429I11Rik), and homozygous JhylacZ/lacZ mice develop a rapidly progressive juvenile hydrocephalus (Appelbe et al., 2013). Molecular analysis of the ependymal cells in JhylacZ/lacZ mice was performed using a cell-type specific marker approach to assess the expression of markers involved in vital cellular processes of these cells. JhylacZ/lacZ mice display abnormal ependymal cell differentiation with ventricular ependyma retaining an unorganized and multi-layered morphology, representative of immature ependymal cells. Morphological and molecular analysis of the ependyma demonstrated a delay rather than a block in… Advisors/Committee Members: Orenic, Teresa V. (advisor), Schmidt, Jennifer V. (committee member), Okkema, Peter (committee member), Tyner, Angela (committee member), Leonard, John (committee member).

Subjects/Keywords: Hydrocephalus; choroid plexus; ependymal cells; JHY

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Muniz-Talavera, H. (2016). Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/20870

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Muniz-Talavera, Hilmarie. “Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus.” 2016. Thesis, University of Illinois – Chicago. Accessed March 07, 2021. http://hdl.handle.net/10027/20870.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Muniz-Talavera, Hilmarie. “Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus.” 2016. Web. 07 Mar 2021.

Vancouver:

Muniz-Talavera H. Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10027/20870.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Muniz-Talavera H. Defining the Role of the Mouse Jhy Gene in the Ependyma and Choroid Plexus. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/20870

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

IUPUI

4. Preston, Daniel. TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production.

Degree: 2019, IUPUI

URL: http://hdl.handle.net/1805/21335

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Indiana University-Purdue University Indianapolis (IUPUI)

Hydrocephalus is a disease characterized by an increase in cerebrospinal fluid (CSF) in the ventricles of the brain. This manifests… (more)

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Indiana University-Purdue University Indianapolis (IUPUI)

Hydrocephalus is a disease characterized by an increase in cerebrospinal fluid (CSF) in the ventricles of the brain. This manifests as a result of either overproduction or underabsorption of CSF leading to increases in pressure, swelling and loss of brain matter. Current treatments for this disease include surgical interventions via the introduction of shunts or endoscopic third ventriculostomy, both of which aim to redirect flow of CSF in to another cavity for absorption. Limited pharmacotherapies are available in the treatment of hydrocephalus, and there exists a clinical need for drug therapies, which can ameliorate the pathophysiology associated with hydrocephalus and ventriculomegaly. CSF is produced primarily by the choroid plexus (CP), found in the ventricles of the brain. Composed of a high resistance epithelium surrounding a capillary network, the CP epithelium acts as a barrier, regulating ion transport between the CSF and blood. Transient Receptor Potential Vanilloid-4 (TRPV4) is a nonselective Ca2+-permeable cation channel expressed in the CP which is being investigated for its role in CSF production. To study hydrocephalus, we utilize two model systems; the TMEM67-/- Wpk rat, and the PCP-R cell line. The Wpk rat model is used to study the effects of drug intervention on the development and progression of hydrocephalus. The PCP-R cell line is utilized for studies which aim to understand the mechanisms by which CSF is produced. Using Ussing chamber electrophysiology, we are able to study the role of specific channels, transporters and modulators in driving epithelial ion flux across the CP. This research aims to establish a role for TRPV4 in production and regulation of CSF, and to interrogate a mechanism by which this ion transport occurs. The chapters that follow describe components of the pathway by which TRPV4 is activated and ion flux is stimulated.

Advisors/Committee Members: Blazer-Yost, Bonnie, Belecky-Adams, Teri, Clack, James, Berbari, Nick.

Subjects/Keywords: Hydrocephalus; Choroid Plexus; CSF; Cerebrospinal Fluid; TRPV4

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Preston, D. (2019). TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/21335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Preston, Daniel. “TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production.” 2019. Thesis, IUPUI. Accessed March 07, 2021. http://hdl.handle.net/1805/21335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Preston, Daniel. “TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production.” 2019. Web. 07 Mar 2021.

Vancouver:

Preston D. TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production. [Internet] [Thesis]. IUPUI; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1805/21335.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Preston D. TRPV4 in the Choroid Plexus Epithelium: Pathway Analysis and Implications for Cerebrospinal Fluid Production. [Thesis]. IUPUI; 2019. Available from: http://hdl.handle.net/1805/21335

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Oliveira Moreira, Vanessa. OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes.

Degree: Docteur es, Neurosciences, 2019, Sorbonne université

URL: http://www.theses.fr/2019SORUS286

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Le plexus choroïde est une structure fortement vascularisée. Situé dans les ventricules cérébraux, il est responsable de la production du liquide céphalorachidien (LCR) contenant un… (more)

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Le plexus choroïde est une structure fortement vascularisée. Situé dans les ventricules cérébraux, il est responsable de la production du liquide céphalorachidien (LCR) contenant un large panel de molécules essentielles aux diverses fonctions cérébrales. Ma thèse a porté sur la découverte de deux protéines exprimées par le plexus choroïde qui modulent la neurogenèse adulte chez la souris : homéoprotéine OTX2 et la protéine précurseur de l'amyloide (PPA). OTX2 est fortement exprimé dans le plexus choroïde et est sécrété dans le LCR. Nous avons découvert qu'il peut réguler la neurogenèse chez la souris adulte en transférant depuis les cellules du plexus choroïde dans les astrocytes de soutien de la zone sous-ventriculaire (ZSV) et de la chaine migratoire rostral (CMR). Nous avons mis en évidence un mécanisme potentiel par lequel OTX2 modifie l'expression des protéines de la matrice extracellulaire spécifiques des astrocytes, ce qui ralentit la migration des neuroblastes dans la CMR et diminue le nombre de nouveaux neurones dans le bulbe olfactif. De plus, grâce à une analyse transcriptomique du plexus choroïde à partir de modèles de souris knockout conditionnels et constitutifs pour Otx2 chez l'adulte, nous avons montré qu'OTX2 régule l'expression des gènes impliqués dans de nombreuses fonctions homéostatiques. Associé à une approche d’élimination virale d'OTX2, nous avons confirmé son implication dans la réponse immunitaire, la réponse au stress oxydatif, l’épissage et homéostasie des métaux. Ces résultats démontrent qu'OTX2 est un régulateur majeur des fonctions essentielles du plexus choroïde pour le maintien de homéostasie cérébrale. Enfin, la PPA est également fortement exprimée dans le plexus choroïde et son domaine soluble est libéré dans le LCR. Lorsque nous avons réduit les concentrations de PPA dans le plexus choroïde chez la souris adulte, nous avons observé une diminution de la prolifération dans les deux niches neurogéniques : la ZSV et le gyrus denté de l'hippocampe (GD). L'augmentation des concentrations de PPA a entraîné une prolifération accrue dans ces deux niches. Pour mesurer l'impact sur les fonctions cérébrales de la PPA extracellulaire provenant du plexus choroïde, nous avons exprimé spécifiquement dans le plexus choroïde de souris sauvage adultes, la PPA humaine porteuse de mutations SwInd de la maladie d'Alzheimer familiale. Après 6 mois, la prolifération dans la ZSV et le GD a été considérablement réduite. Après 12 mois, des souris ont montré des déficits d'apprentissage inverse dans des expériences de comportement en champ ouvert et ont montré une plasticité altérée dans la potentialisation à long terme (PLT) mesurée par stimulation à haute fréquence de tranches d'hippocampe.

The choroid plexus is a richly vascularized structure located in brain ventricles and is responsible for the production of cerebrospinal fluid (CSF) containing a large panel of molecules essential for various brain functions. My thesis focused on the discovery of two proteins expressed by the choroid plexus that…

Advisors/Committee Members: Di Nardo, Ariel (thesis director).

Subjects/Keywords: Neurogenèse; Plexus choroïde; OTX2; LCR; Homéostasie; PPA; Neurogenesis; Choroid plexus; OTX2; CSF; Homeostasis; APP; 573.848

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oliveira Moreira, V. (2019). OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes. (Doctoral Dissertation). Sorbonne université. Retrieved from http://www.theses.fr/2019SORUS286

Chicago Manual of Style (16^th Edition):

Oliveira Moreira, Vanessa. “OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes.” 2019. Doctoral Dissertation, Sorbonne université. Accessed March 07, 2021. http://www.theses.fr/2019SORUS286.

MLA Handbook (7^th Edition):

Oliveira Moreira, Vanessa. “OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes.” 2019. Web. 07 Mar 2021.

Vancouver:

Oliveira Moreira V. OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes. [Internet] [Doctoral dissertation]. Sorbonne université; 2019. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2019SORUS286.

Council of Science Editors:

Oliveira Moreira V. OTX2 homeoprotein and amyloid protein in adult neurogenesis and choroid plexus functions : Homéoprotéine OTX2 et protéine précurseur de l'amyloïde dans la neurogenèse adulte et les fonctions des plexus choroïdes. [Doctoral Dissertation]. Sorbonne université; 2019. Available from: http://www.theses.fr/2019SORUS286

Boston University

6. Lun, Melody. Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain.

Degree: PhD, Pathology, 2016, Boston University

URL: http://hdl.handle.net/2144/19054

► Fundamental to mammalian brain development is the integration of cell intrinsic and extrinsic signals that direct the proliferation and differentiation of neural stem cells. Precise… (more)

▼ Fundamental to mammalian brain development is the integration of cell intrinsic and extrinsic signals that direct the proliferation and differentiation of neural stem cells. Precise expression of transcription factors together with other intracellular components instruct progenitor cell fate, whereas interaction with extracellular signaling factors refines this process. We have elucidated the composition of the cerebrospinal fluid that is the source of multiple extrinsic cues during brain development. The choroid plexus, a highly vascularized tissue located in each ventricle of the brain, actively secretes cerebrospinal fluid. By RNA sequencing, we obtained transcriptome data on the choroid plexi from lateral and fourth ventricles of the mouse brain and discovered that they include transcripts unique to each tissue. Transcription factor expression in the macaque and human choroid plexi suggests that positional identities of these tissues are conserved in the primate brain. Based on transcriptional results, we defined the choroid plexus secretome, a prediction of secreted factors from the choroid plexus. By quantitative mass spectrometry, we detected proteins secreted by each choroid plexus, and comparison of these proteomic results with transcriptional profiling suggests that choroid plexus transcriptomes contribute to availability of regionalized cerebrospinal fluid factors during development. In the second part of my dissertation research, I studied the role of DNA repair mechanisms in regulating neural stem cells. These studies focused on DNA LigaseIV, an essential component of DNA double-stranded break repair, during cerebral cortical development. Deficiency of LigaseIV activity caused by a missense mutation leads to LigaseIV syndrome, in which a key clinical feature is microcephaly. Using the Lig4 R278H mouse mutant, we found increased cell death in the developing cortex, contributing to reduced cortical thickness and cellularity in the anterior cerebral cortex. These results indicate that DNA LigaseIV is essential for proper cortical development. Together, these findings illustrate the complexity of regulatory mechanisms that guide brain development, requiring the integration of mechanisms from within and outside the cell. We have investigated two such mechanisms, extrinsic cues from regionalized cerebrospinal fluid and DNA LigaseIV. These results should provide greater insight into mechanisms of normal brain development and neuropathological states.

Subjects/Keywords: Neurosciences; Cerebrospinal fluid; Choroid plexus; Cortical development; Ligase IV; Proteome; Transcriptome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lun, M. (2016). Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/19054

Chicago Manual of Style (16^th Edition):

Lun, Melody. “Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain.” 2016. Doctoral Dissertation, Boston University. Accessed March 07, 2021. http://hdl.handle.net/2144/19054.

MLA Handbook (7^th Edition):

Lun, Melody. “Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain.” 2016. Web. 07 Mar 2021.

Vancouver:

Lun M. Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain. [Internet] [Doctoral dissertation]. Boston University; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2144/19054.

Council of Science Editors:

Lun M. Regionalized choroid plexus-cerebrospinal fluid factors and effect of DNA Ligase IV deficiency in the developing mammalian brain. [Doctoral Dissertation]. Boston University; 2016. Available from: http://hdl.handle.net/2144/19054

IUPUI

7. Ahmed, Shehab. TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus.

Degree: 2016, IUPUI

URL: http://hdl.handle.net/1805/11821

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Indiana University-Purdue University Indianapolis (IUPUI)

Hydrocephalus is a medical condition characterized by a buildup of cerebrospinal fluid which causes hydrostatic pressure to increase resulting neuronal… (more)

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Indiana University-Purdue University Indianapolis (IUPUI)

Hydrocephalus is a medical condition characterized by a buildup of cerebrospinal fluid which causes hydrostatic pressure to increase resulting neuronal destruction and can ultimately cause death. Hydrocephalus is seen in both the pediatric population and adults. Treatment of hydrocephalus usually involves surgical placement of a relocation system to drain the fluid into the abdominal cavity. Hydrocephalus may be caused by mechanical obstruction of the outflow of CSF from the ventricles or by faulty reabsorption. It can be also caused by CSF overproduction by the choroid plexus found in the lateral, third, and fourth ventricles of the brain. The choroid plexus is composed of a high resistance monolayer epithelium which surrounds a network of capillaries. Its primary function is to regulate transport of ions and water that control the production and movement of CSF. Therefore it is important to understand the mechanism of CSF production by the choroid plexus. Recently, a stable porcine choroid plexus (PCP-R) epithelial cell line with a high transepithelial resistance (TER) was developed that provides an important model to study regulation of CSF production. Ussing style electrophysiology was used to measure short circuit current (SCC) to characterize stimulated transepithelial ion transport in confluent PCP-R cells. GSK1016790, a TRPV4 agonist, was used to understand the role of TRPV4 in CSF production by the choroid plexus using PCP-R cell model. TRPV4 activation produces a sustained ion transport response that is consistent with an increase in cation secretion and/or anion absorption which is accompanied by a reversible decrease in TER. The effect of the agonist on both SCC and TER was blocked by HC067047, a TRPV4 antagonist, showing that the sustained ion transport and TER change is TRPV4 specific. TRPV4 mediated ion flux was inhibited by CFTR inhibitor II GlyH-101, a cell permeable inhibitor of the cAMP activated chloride channel CFTR, when added on either side of the membrane and was not accompanied by a TER reversal which showed that CFTR is activated by TRPV4 mediated ion flux. TMEM16A, a calcium activated chloride channel, was speculated to be located in that basal membrane as T16Ainh-AO1, a membrane permeable TMEM16A inhibitor, reversed the TRPV4 mediated ion flux when added on either side of the membrane. Slight reversal in TER was observed when T16Ainh-AO1 was added on the apical side. Apamin, a differential inhibitor of calcium activated small conductance potassium channel 1, 2 and 3 (SK1, SK2 and SK3) had no effect on the TRPV4 mediated ion flux. Whereas, fluoxetine, a membrane permeable inhibitor of SK1, SK2 and SK3 channel, inhibited the TRPV4 mediated ion flux and TER change. Bumetanide, an inhibitor of the sodium-potassium-chloride cotransporter reversed TRPV4 mediated ion flux when added on the apical membrane but not on the basal membrane indicating a possible K+ secretion via SK1 and/or SK4/IK channels and Cl- absorption through CFTR…

Advisors/Committee Members: Blazer-Yost, Bonnie.

Subjects/Keywords: TRPV4; Choroid Plexus; Cerebrospinal fluid; CSF; HC067047; GSK1016790

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahmed, S. (2016). TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/11821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ahmed, Shehab. “TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus.” 2016. Thesis, IUPUI. Accessed March 07, 2021. http://hdl.handle.net/1805/11821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ahmed, Shehab. “TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus.” 2016. Web. 07 Mar 2021.

Vancouver:

Ahmed S. TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus. [Internet] [Thesis]. IUPUI; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1805/11821.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ahmed S. TRPV4 and cAMP Mediated Ion Transport in the Porcine Choroid Plexus. [Thesis]. IUPUI; 2016. Available from: http://hdl.handle.net/1805/11821

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Zuba, Vincent. Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA).

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2019, Normandie

URL: http://www.theses.fr/2019NORMC410

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L’activateur tissulaire du plasminogène (tPA) est une protéase initialement découverte dans le sang pour son rôle fibrinolytique. C’est pour cette fonction que le tPA recombinant… (more)

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L’activateur tissulaire du plasminogène (tPA) est une protéase initialement découverte dans le sang pour son rôle fibrinolytique. C’est pour cette fonction que le tPA recombinant est utilisé pour traiter la phase aigüe de l’accident vasculaire cérébral (AVC) ischémique, même s’il présente quelques limites. Le tPA exogène peut passer du compartiment vasculaire au parenchyme cérébral où il peut influencer des processus physiologiques, et participer au devenir neuronal, notamment aggraver la mort neuronale lors d’un AVC ischémique. Le laboratoire a montré que le tPA peut traverser la barrière-hémato-encéphalique (BHE), par transcytose au travers des cellules endothéliales de la BHE et cela sous le contrôle des récepteurs LRP1 (Low density lipoprotein receptor-related protein 1). D’autres barrières existent au sein du système nerveux central notamment la barrière sang-liquide cérébrospinal (BSLCS), formée par les plexus choroïdes (PCs). Les PCs sont une route de migration pour les cellules inflammatoires et le LCS peut véhiculer des solutés, via les espaces péri-artériels, vers le parenchyme cérébral. Ainsi, dans notre première étude, nous avons testé l’hypothèse d’un passage du tPA vasculaire par les PCs. Pour cela, nous avons produit un tPA traçable in vivo et in vitro. Nous avons commencé par étudier la distribution du tPA suite à une injection intraveineuse (IV) avec comme focus les PCs et le LCS. Nos résultats montrent que le tPA exogène, suite à une injection IV, est retrouvé de manière séquentielle dans les PCs puis dans le LCS. Le tPA est donc capable de traverser les PCs. Nous avons alors développé un modèle de culture primaire de cellules épithéliales de PCs (CPECs) de souris pour disséquer le(s) mécanisme(s) sous-jacents à l’internalisation du tPA par les CPECs. Ce modèle nous a permis de montrer que l’internalisation du tPA par les CPECs est un phénomène actif, médié par un membre de la famille des récepteurs LRP, mais qui n’est ni LRP1, ni LRP2. Nous avons également mis en évidence la nécessité du domaine Finger du tPA pour son internalisation par les CPECs. Une étude préliminaire dans un modèle d’AVC suggère que l’ischémie modifie la cinétique de passage du tPA, puisqu’il y a plus de tPA dans les PCs des souris ischémiées que les souris non ischémiées.Dans une deuxième étude nous nous sommes intéressés à l’effet du tPA endogène sur les PCs. Nous montrons que l’absence de tPA endogène n’influence ni la morphologie des PCs, ni la diffusion du LCS. De plus, nous montrons que cette absence de tPA n’influence pas le nombre de macrophages et de lymphocytes T dans les PCs en conditions basales.

Tissue-type plasminogen activator (tPA) is a protease initially discovered in the blood for its fibrinolytic role. Accordingly, recombinant tPA has become the gold standard to treat the acute phase of ischemic stroke, despite some limitations. Exogenous tPA can switch from the vascular compartment to the brain parenchyma, where it can influence physiological processes, and participate in neuronal fate, including a…

Advisors/Committee Members: Ali, Carine (thesis director).

Subjects/Keywords: Accident vasculaire cérébral; Choroid plexus; Stroke; Tissue-type plasminogen activator

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APA (6^th Edition):

Zuba, V. (2019). Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA). (Doctoral Dissertation). Normandie. Retrieved from http://www.theses.fr/2019NORMC410

Chicago Manual of Style (16^th Edition):

Zuba, Vincent. “Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA).” 2019. Doctoral Dissertation, Normandie. Accessed March 07, 2021. http://www.theses.fr/2019NORMC410.

MLA Handbook (7^th Edition):

Zuba, Vincent. “Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA).” 2019. Web. 07 Mar 2021.

Vancouver:

Zuba V. Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA). [Internet] [Doctoral dissertation]. Normandie; 2019. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2019NORMC410.

Council of Science Editors:

Zuba V. Les plexus choroïdes : une entrée au niveau cérébral pour l'activateur tissulaire du plasminogène (tPA) : Choroid plexus : an entry to the brain for tissue-type plasminogen activator (tPA). [Doctoral Dissertation]. Normandie; 2019. Available from: http://www.theses.fr/2019NORMC410

9. Thompson, Derick. Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes.

Degree: PhD, Biomedical Sciences, 2020, University of North Dakota

URL: https://commons.und.edu/theses/3305

► Lyme disease, caused by the spirochete Borrelia burgdorferi (Bb), is the most commonly reported vector-borne disease in the United States – with 30,000 cases… (more)

▼ Lyme disease, caused by the spirochete Borrelia burgdorferi (Bb), is the most commonly reported vector-borne disease in the United States – with 30,000 cases being reported to the CDC annually, though it is estimated that 300,000 individuals are infected each year in the U.S [1–4]. Due to the medical treatment of the disease, this equates to an estimated 712 million - 1.3 billion in medical costs each year [5]. Conclusively, due to the continued geographical spread and increasing incidence rate, Lyme disease is becoming a greater public health threat throughout the world. The symptoms of Lyme disease can range from erythema migrans to more systematic disorders such as arthritis and neurological complications, termed neuroborreliosis [6,7]. Manifestations of neuroborreliosis include radiculoneuritis, meningitis, and facial palsy [8–10]. Interestingly, B. burgdorferi does not produce any known toxins, and it is thought that the resulting host immune response leads to cellular and tissue damage associated with clinical symptoms. Although many individuals will be effectively treated through the administration of antibiotics, up to 20% of patients will experience on-going symptoms termed Post-treatment Lyme Disease Syndrome (PTLDS). PTLDS is marked by persistent musculoskeletal pain and neurological complications. Inflammatory states have been associated to these symptoms with the invasion of peripheral immune cells and an increase of inflammatory cytokines. Furthermore, the neurological complications of PTLDS has been associated with an increase in glial inflammation. It is well-documented that B. burgdorferi is capable of penetrating into the central nervous system (CNS); however, it is unknown how and where the bacterium does so. Additionally, the exact pathogenetic mechanisms of neuroborreliosis and PTLDS are poorly understood. The work within this dissertation aims to provide novel insight into these gaps in knowledge. This dissertation is laid out into three sections relating to understanding the pathogenesis of the neurological effects of Lyme disease. In the first study, we aimed to provide an explanation for the dissemination of B. burgdorferi and peripheral immune cells into the central nervous system. Clinical presentations of neuroborreliosis is associated with an increase of peripheral immune cells, inflammatory chemokines, and live B. burgdorferi in the cerebrospinal fluid (CSF). To this end, we sought to investigate a direct route from hematogenous dissemination into the CSF. The choroid plexus (CP) is a structure within the ventricles of the brain that is responsible for the production of CSF, the formation of the blood-CSF barrier, and regulation of the immune response. This barrier allows for the exchange of specific nutrients, waste, and peripheral immune cells between the blood stream and CSF. We hypothesize that during infection of the choroid plexus, Borrelia burgdorferi will induce an immune response conducive to the chemotaxis of immune… Advisors/Committee Members: Catherine A. Brissette, John A. Watt.

Subjects/Keywords: Astrocytes; Bioinformatics; Choroid Plexus; Epigenetics; Lyme Disease; Neuroscience

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APA (6^th Edition):

Thompson, D. (2020). Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes. (Doctoral Dissertation). University of North Dakota. Retrieved from https://commons.und.edu/theses/3305

Chicago Manual of Style (16^th Edition):

Thompson, Derick. “Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes.” 2020. Doctoral Dissertation, University of North Dakota. Accessed March 07, 2021. https://commons.und.edu/theses/3305.

MLA Handbook (7^th Edition):

Thompson, Derick. “Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes.” 2020. Web. 07 Mar 2021.

Vancouver:

Thompson D. Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes. [Internet] [Doctoral dissertation]. University of North Dakota; 2020. [cited 2021 Mar 07]. Available from: https://commons.und.edu/theses/3305.

Council of Science Editors:

Thompson D. Investigation Of The Neurological Manifestations Of Lyme Disease And The Impact Of Borrelia Burgdorferi On The Epigenetic Landscape Of Astrocytes. [Doctoral Dissertation]. University of North Dakota; 2020. Available from: https://commons.und.edu/theses/3305

University of Toronto

10. Merino, Diana M. Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours.

Degree: PhD, 2015, University of Toronto

URL: http://hdl.handle.net/1807/71585

► Choroid plexus tumours (CPTs) are rare intraventricular neoplasms, most commonly found in children, with variable clinical presentation and patient outcome. CPT biology is poorly understood… (more)

Subjects/Keywords: brain tumours; cancer genomics; choroid plexus tumours; pediatric cancer; 0369

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Merino, D. M. (2015). Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/71585

Chicago Manual of Style (16^th Edition):

Merino, Diana M. “Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours.” 2015. Doctoral Dissertation, University of Toronto. Accessed March 07, 2021. http://hdl.handle.net/1807/71585.

MLA Handbook (7^th Edition):

Merino, Diana M. “Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours.” 2015. Web. 07 Mar 2021.

Vancouver:

Merino DM. Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours. [Internet] [Doctoral dissertation]. University of Toronto; 2015. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1807/71585.

Council of Science Editors:

Merino DM. Choroid Plexus Tumours: Elucidating the Genomic Complexity of Rare Pediatric Brain Tumours. [Doctoral Dissertation]. University of Toronto; 2015. Available from: http://hdl.handle.net/1807/71585

University of Melbourne

11. Liddelow, Shane Antony. Molecular transfer across blood-cerebrospinal fluid interface in early brain development.

Degree: 2011, University of Melbourne

URL: http://hdl.handle.net/11343/35808

► The choroid plexus is the site of the blood-cerebrospinal fluid barrier and restricts the exchange of molecules between the two compartments. The normal development of… (more)

▼ The choroid plexus is the site of the blood-cerebrospinal fluid barrier and restricts the exchange of molecules between the two compartments. The normal development of the lateral ventricular plexus was studied in neonatal opossum (Monodelphis domestica) using BrdU labelling. Results show that choroid plexus cells arise from the neuroependymal wall of the ventricles, with new epithelial cells only added to the structure on the dorsal (upper) side. Plexus cells are long-lived and no apoptosis was seen, even in senescent animals. Early in development cerebrospinal fluid (CSF) contains very high concentrations of protein, most likely generated by transfer from plasma across choroid plexus epithelial cells. Results showed that about 5–10% of plexus epithelial cells are involved in unidirectional transfer of plasma proteins from blood to CSF; about 0.5% of cells transfer passive markers bidirectionally, and a small percentage of cells transfer both. The specificity of the protein transfer was greatest in younger animals and decreased with age. Physiological control of protein transfer across plexus epithelial cells was investigated by injecting adult plasma into Monodelphis pups at different postnatal ages. Concentrations of total and individual plasma proteins were estimated and volumes of the lateral ventricular system measured. Injections of adult plasma resulted in increased total protein concentration in the CSF within 6–24 hours in older, but not younger animals due to ventricular expansion at early ages only. Protein content in CSF increased at all ages. The concentration of individual proteins was differentially altered and numbers of choroid plexus cells immunopositive for them mirrored these changes. Injection of a foreign protein, bovine fetuin, resulted in an increase in CSF protein content and the exogenous protein was detected both in the CSF and within protein-transferring plexus cells. Further investigation of the molecular basis of this transfer was conducted using a microarray screen of E15 and adult mouse lateral ventricular choroid plexus. Comparison of datasets revealed many genes that were differentially expressed, including numerous receptor and protein transporters. One putative plasma protein receptor, osteonectin, was localized to the basolateral (blood) side of the choroid plexus epithelial cells. These combined results suggest that specific transporters responsible for protein transfer at the blood/CSF barrier may be present, raising the possibility of designing specific macromolecular delivery systems into the brain via the choroid plexus epithelial cells and the CSF, especially during development.

Subjects/Keywords: choroid plexus; blood-CSF barrier; protein transport; Monodelphis domestica; development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liddelow, S. A. (2011). Molecular transfer across blood-cerebrospinal fluid interface in early brain development. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/35808

Chicago Manual of Style (16^th Edition):

Liddelow, Shane Antony. “Molecular transfer across blood-cerebrospinal fluid interface in early brain development.” 2011. Doctoral Dissertation, University of Melbourne. Accessed March 07, 2021. http://hdl.handle.net/11343/35808.

MLA Handbook (7^th Edition):

Liddelow, Shane Antony. “Molecular transfer across blood-cerebrospinal fluid interface in early brain development.” 2011. Web. 07 Mar 2021.

Vancouver:

Liddelow SA. Molecular transfer across blood-cerebrospinal fluid interface in early brain development. [Internet] [Doctoral dissertation]. University of Melbourne; 2011. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11343/35808.

Council of Science Editors:

Liddelow SA. Molecular transfer across blood-cerebrospinal fluid interface in early brain development. [Doctoral Dissertation]. University of Melbourne; 2011. Available from: http://hdl.handle.net/11343/35808

12. Aquino, Mayra. Regulation of Zinc Transport in the Choroid Plexus.

Degree: MS, Food Science and Technology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/153201

► The choroid plexus epithelium forms the blood-cerebrospinal fluid barrier, but also accumulates and transports nutritive minerals, such as zinc, into and out of the cerebrospinal… (more)

▼ The choroid plexus epithelium forms the blood-cerebrospinal fluid barrier, but also accumulates and transports nutritive minerals, such as zinc, into and out of the cerebrospinal fluid. The goal of this thesis was to analyze interdependent regulation of zinc transporters with metallothionein as the choroid plexus epithelium adapts to increases or decreases in extracellular zinc. My first objective was to characterize time-dependent changes in zinc transporter and MT-1 expression as extracellular zinc was pharmacologically depleted or supplemented. My second objective was to characterize changes in zinc transporter and MT-1 expression in response to exposure to prolactin. My experimental approach was to analyze gene expression of ZnT1, Zip1, Zip6, MT-1 and carbonic anhydrase (CA-2) in primary cell cultures of neonatal rat choroid plexus and isolated tissues in which extracellular zinc was depleted with 10 μM diethylene triamine pentaacetic acid or supplemented with 25 μM ZnCl_(2) for 48 h. Gene expression was analyzed by fluorescence quantitative real-time polymerase chain reaction. Zinc accumulation studies indicate choroid plexus cells maintain capacity to accumulate zinc, even when zinc is chelated. In cells, zinc depletion decreased expression of MT-1 and ZnT1 at 3 h and increased Zip1 expression; Zip6 expression fluctuated. In isolated tissues, zinc depletion down-regulated MT-1 and ZnT1 expression, while up-regulating Zip1 and Zip6 expression. In cells, zinc supplementation induced MT-1, ZnT1 and Zip6 expression at 3 h. Zip1 expression decreased at 3 h. In isolated tissues zinc supplementation up-regulated MT-1 and ZnT1 expression, but did not alter Zip1 and Zip6 expression. These data indicate there is coordinated regulation of MT-1 and zinc transporters as extracellular zinc altered. Prolactin up-regulated gene expression of CA-2, MT-1, ZnT-1 and Zip6 in choroid plexus cells. The JAK/STAT inhibitor AG-490 increased CA-2 and MT-1 expression, but decreased ZnT1 and Zip6 expression. AG-490 further increased expression of CA-2 and MT-1 in prolactin treated cells. This suggests the JAK/STAT signaling pathway might tonically suppress basal expression of MT-1 and CA-2. AG-490 partially reversed up-regulation of ZnT-1 and Zip6 expression by prolactin. These data indicate there is a coordinated regulation of MT-1 and zinc transporters during extracellular zinc depletion or supplementation. Advisors/Committee Members: Harris, Kerri B. (advisor), Villalobos, Alice R.A. (advisor), Gomes, Carmen L. (committee member).

Subjects/Keywords: Zinc; Choroid Plexus

…choroid plexus, which forms the blood-cerebrospinal fluid barrier. There are two primary… …capillaries. The choroid plexus epithelium forms the blood-CSF barrier, which is separate and… …distinct from the blood-brain-barrier. There are four choroid plexus tissues, one in each lateral… …third and fourth ventricle of the brain. The capillaries of choroid plexus tissue are… …x28;22, 29). The choroid plexus epithelium produces CSF, which is functionally continuous…

11 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aquino, M. (2014). Regulation of Zinc Transport in the Choroid Plexus. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153201

Chicago Manual of Style (16^th Edition):

Aquino, Mayra. “Regulation of Zinc Transport in the Choroid Plexus.” 2014. Masters Thesis, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/153201.

MLA Handbook (7^th Edition):

Aquino, Mayra. “Regulation of Zinc Transport in the Choroid Plexus.” 2014. Web. 07 Mar 2021.

Vancouver:

Aquino M. Regulation of Zinc Transport in the Choroid Plexus. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/153201.

Council of Science Editors:

Aquino M. Regulation of Zinc Transport in the Choroid Plexus. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153201

13. Planques, Anabelle. Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis.

Degree: Docteur es, Neurosciences, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066501

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La neurogenèse adulte permet la formation de nouveaux neurones dans les bulbes olfactifs de la souris. Les propriétés des cellules souches neurales situées dans la… (more)

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La neurogenèse adulte permet la formation de nouveaux neurones dans les bulbes olfactifs de la souris. Les propriétés des cellules souches neurales situées dans la zone sous-ventriculaire (ZSV) et des précurseurs sont régulées par la niche contenant des cellules de support et une matrice extracellulaire (MEC). Des facteurs contenus dans le liquide cérébrospinal (LCS), produits par les plexus choroïdes (PC), contrôlent aussi la niche. L'homéoprotéine Otx2 est secrétée dans le LCS par les PC, et internalisée spécifiquement par certaines cellules du parenchyme cérébral. Otx2 est impliquée dans différentes étapes du développement du cerveau, dont celui des PC, et peut agir de manière non-cellulaire-autonome. Ma thèse vise à comprendre comment Otx2 régule les fonctions des PC et participe à la neurogenèse adulte. Grâce à des études génomiques d'un modèle murin knockdown (KD) d'Otx2 dans les PC adultes, nous avons montré que (i) les PC de différents ventricules présentent des profils d'expression différents (ii) le KD d'Otx2 modifie l'expression de gènes impliqués dans des fonctions importantes des PC (iii) la dérégulation de certains gènes après KD est spécifique d'un type de PC. Une étude protéomique suggère (iv) qu'Otx2 pourrait être impliquée à d'autres niveaux que la régulation transcriptionnelle. L'étude de la neurogenèse adulte dans des modèles murins KD d'Otx2 nous a permis de montrer que (i) l'expression d'Otx2 dans les PC régule la neurogenèse adulte (ii) Otx2 transfère dans les astrocytes de la ZSV (iii) le transfert d'Otx2 est suffisant pour réguler la neurogenèse (iv) le KD d'Otx2 dans les PC modifie l'expression de protéines de la MEC secrétées par les astrocytes.

Adult neurogenesis in mice involves neural stem cells in the subventricular zone (SVZ) whose progenitors integrate into the olfactory bulbs. The neurogenic niche, which contains supporting cells and extracellular matrix (ECM), regulates the properties (proliferation, migration and differentiation) of progenitor cells. This niche is influenced by factors from cerebrospinal fluid (CSF), which is produced by the choroid plexus (CP) in the brain ventricles. The Otx2 homeoprotein transcription factor is secreted into CSF by CP, and taken up by a specific subset of cells within the brain parenchyma. Otx2 is involved in various stages of brain development, including CP development, and has non-cell autonomous functions. The aim of my thesis is to understand how Otx2 regulates adult CP function and participates in adult SVZ neurogenesis. Through genomic studies, we investigated the consequence of Otx2 knockdown (KD) in adult CP and found: (i) adult CP from different ventricles exhibit different expression profiles; (ii) Otx2 KD alters the expression of genes with important CP functions; and (iii) deregulation of certain genes after Otx2 KD can be CP specific. Through proteomics studies, we found that (iv) adult Otx2 could be involved in functions beyond transcriptional regulation, such as RNA processing.To evaluate the role of Otx2 in SVZ neurogenesis,…

Advisors/Committee Members: Di Nardo, Ariel (thesis director).

Subjects/Keywords: Otx2; Plexus choroïdes; Neurogenèse adulte; Astrocytes; Matrice extracellulaire; Fonction non-cellulaire autonome; Otx2; Choroid plexus; Adult neurogenesis; 573.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Planques, A. (2016). Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066501

Chicago Manual of Style (16^th Edition):

Planques, Anabelle. “Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 07, 2021. http://www.theses.fr/2016PA066501.

MLA Handbook (7^th Edition):

Planques, Anabelle. “Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis.” 2016. Web. 07 Mar 2021.

Vancouver:

Planques A. Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2016PA066501.

Council of Science Editors:

Planques A. Expression et sécrétion d'Otx2 par les plexus choroïdes, nouvelle évidence d'un contrôle non-cellulaire- autonome de la neurogenèse adulte. Rôles physiologiques d’Otx2 : Expression and secretion of Otx2 by choroid plexus, new evidence for non-cell autonomous regulation of adult neurogenesis. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066501

14. Arnaud, Karen. Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease.

Degree: Docteur es, Neurosciences, 2016, Université Pierre et Marie Curie – Paris VI

URL: http://www.theses.fr/2016PA066214

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Le vieillissement et la dégénérescence du cerveau, associés à des déficits cognitifs, comportementaux et neurologiques, représentent aujourd'hui un problème majeur de santé publique. L'une des… (more)

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Le vieillissement et la dégénérescence du cerveau, associés à des déficits cognitifs, comportementaux et neurologiques, représentent aujourd'hui un problème majeur de santé publique. L'une des principales maladies liées à l'âge est la maladie d'Alzheimer (MA). L'une des caractéristiques de la MA est l'apparition de plaques amyloïdes, résultant de l'agrégation du peptide ßA4. Physiologiquement, le précurseur de la protéine amyloïde (APP) est clivé par une alpha-sécrétase qui génère un fragment soluble de l'APP (sAPP), important pour la formation de nouvelles cellules nerveuses (neurogenèse). Ce clivage en prévient deux autres, par les béta- et gamma-sécrétases, impliqués dans la MA, et conduisant à la formation du ßA4 toxique. Une analyse du plexus choroïde (PCh) a mis en évidence la forte expression de l’APP par cette structure cérébrale. Le PCh est une structure facilement accessible et produisant le liquide cérébro-spinal : son impact peut donc être répercuté à l’ensemble du cerveau. Il pourrait être une source cérébrale importante d’APP, et contribuer fortement à la pathologie. Mon projet de thèse s'inscrivait dans la possibilité de réguler génétiquement l'expression des formes sauvages et mutées de l'APP au niveau de cette source, et suivre les conséquences sur la neurogenèse adulte et la formation des plaques amyloïdes, marqueur histopathologique de la MA. Par l’utilisation de la thérapie génique pour moduler l’expression de l’APP dans les PCh, nous avons confirmé l’importance de l’APP soluble provenant des PCh dans la neurogenèse adulte. Les PCh semble être une source importante d’APP dans le cerveau, et pourraient avoir un rôle clé dans la maladie d’Alzheimer.

Aging and degeneration of the brain with cognitive decline and neurologic symptoms are major individual and societal problems. The major age-related brain degeneration disease is Alzheimer’s disease (AD) with about 40 million people affected in 2015.Physiologically, the Amyloid Precursor Protein (APP) is cleaved by an alpha-secretase, releasing soluble APP (sAPP) an important regulator of adult neurogenesis. This cleavage prevents two others in positions beta and gamma that generate the ßA4 toxic peptide, a hallmark of Alzheimer Disease.Next generation RNA-sequencing has revealed that APP is the 16th most expressed genes in the choroid plexus (CP), suggesting that it may be a major source of sAPP and ßA4 in the cerebrospinal fluid (CSF). If so, adult neurogenesis in the SVZ and hippocampus may be regulated by the choroid plexus and impeded in mutations favoring ßA4 production. My thesis project fell under the possibility to regulate App expression in the CP, and follow consequences on adult neurogenesis and plaques formation in AD. Using viral vectors to modulate App expression in the CP, we confirmed the importance of sAPP coming from CP in adult neurogenesis. With so, CP seems to be an important source of APPin the brain, and could have a key role in AD.

Advisors/Committee Members: Prochiantz, Alain (thesis director).

Subjects/Keywords: Alzheimer; Plexus choroïdes; Neurogenèse adulte; Modèles animaux; Physiopathologie; APP; Alzheimer’s Disease; Adult neurogenesis; Choroid plexus; 573.86

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Arnaud, K. (2016). Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease. (Doctoral Dissertation). Université Pierre et Marie Curie – Paris VI. Retrieved from http://www.theses.fr/2016PA066214

Chicago Manual of Style (16^th Edition):

Arnaud, Karen. “Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease.” 2016. Doctoral Dissertation, Université Pierre et Marie Curie – Paris VI. Accessed March 07, 2021. http://www.theses.fr/2016PA066214.

MLA Handbook (7^th Edition):

Arnaud, Karen. “Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease.” 2016. Web. 07 Mar 2021.

Vancouver:

Arnaud K. Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease. [Internet] [Doctoral dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2016PA066214.

Council of Science Editors:

Arnaud K. Sécrétion du précurseur de la protéine amyloïde par les plexus choroïdes : implications dans la neurogenèse adulte et la maladie d'Alzheimer : Secretion of the amyloid precursor protein by the choroid plexus : implications on adult neurogenesis and Alzheimer's disease. [Doctoral Dissertation]. Université Pierre et Marie Curie – Paris VI; 2016. Available from: http://www.theses.fr/2016PA066214

University of Manchester

15. Kamaly-Asl, Ian. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.

Degree: 2011, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:117289

► Prognostic factors come in a variety of forms and may be patient, tumour or environmental related.This thesis examines the interaction of prognostic factors for a… (more)

Subjects/Keywords: Brain Tumours; Prognosis; Vascular Endothelial Growth Factor; Astrocytoma; Choroid Plexus Carcinoma; Medulloblastoma; Ependymoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kamaly-Asl, I. (2011). Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:117289

Chicago Manual of Style (16^th Edition):

Kamaly-Asl, Ian. “Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.” 2011. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:117289.

MLA Handbook (7^th Edition):

Kamaly-Asl, Ian. “Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.” 2011. Web. 07 Mar 2021.

Vancouver:

Kamaly-Asl I. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Mar 07]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:117289.

Council of Science Editors:

Kamaly-Asl I. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:117289

Texas A&M University

16. Francis Stuart, Samantha D. The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus.

Degree: MS, Toxicology, 2014, Texas A&M University

URL: http://hdl.handle.net/1969.1/152822

► Cadmium (Cd) is a toxic heavy metal with no known physiological function in higher order animals. Previous studies in primary cultures of neonatal rat choroid… (more)

▼ Cadmium (Cd) is a toxic heavy metal with no known physiological function in higher order animals. Previous studies in primary cultures of neonatal rat choroid plexus (CP) epithelial cells indicated Cd induced oxidative stress and stimulated apical choline transport, and suggested zinc (Zn) supplementation might abate both oxidative stress and modulation of transport. The objective of this thesis was to elucidate how Zn, a nutritive mineral normally accumulated by CP, attenuated oxidative stress. I hypothesize that Zn, which can function as a pro-antioxidant, abates Cd-induced oxidative stress either by induction of metallothionein-1 (MT-1) or enhancement of glutathione (GSH) biochemistry. Thus, in primary cultures of neonatal rat CP epithelial cells, I characterized the effects of sub-micromolar Cd and efficacy of Zn supplementation to attenuate Cd-induced cellular and oxidative stress without or with manipulation of GSH synthesis. To characterize the Cd-induced stress response, CP epithelial cells were treated with 0 or 500 nM CdCl_(2) in serum-free medium (SFM) for 12 h; samples were collected at 3, 6, 9, and 12 h. Induction of heme oxygenase-1 (HO-1), heat-shock protein 70 (HSP70), and metallothionein-1 (MT-1) in Cd-treated cells was compared to time-matched controls by immunoblot and qRT-PCR analyses. Cd induced the catalytic and modifier subunits of glutamate-cysteine ligase (GCL), the rate-limiting enzyme in GSH synthesis. To elucidate the effects Zn supplementation in Cd-treated cells depleted of GSH, cells were supplemented for 48 h with 0 or 25 μM ZnCl_(2) alone or with 100 μM buthionine sulfoximine (BSO), an inhibitor of GCL, before treatment with 0 or 500 nM CdCl_(2) ± 100 μM BSO ± 10μM ZnCl_(2) in SFM for 12 h. By luminescence assay, intracellular GSH and oxidized glutathione (GSSG) concentrations were measured. Cd increased intracellular GSH and GSSG, but markedly decreased GSH:GSSG ratio. Inhibition of GSH synthesis exacerbated Cd-induced stress. However, Zn supplementation attenuated the stress response irrespective of BSO treatment, as per decreased induction of HSP70. These data indicate that CP adapts to low-dose Cd by up-regulation of stress proteins and GSH synthesis. Zinc supplementation also may attenuate Cd-induced cellular and oxidative stress, but cytoprotection is independent of GSH status. Advisors/Committee Members: Villalobos, Alice R.A. (advisor), Abbott, Louise (committee member), Tian, Yanan (committee member).

Subjects/Keywords: choroid plexus; cadmium; zinc; oxidative stress; glutathione; metallothionein; heat-shock protein 70; hemeoxygenase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Francis Stuart, S. D. (2014). The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/152822

Chicago Manual of Style (16^th Edition):

Francis Stuart, Samantha D. “The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus.” 2014. Masters Thesis, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/152822.

MLA Handbook (7^th Edition):

Francis Stuart, Samantha D. “The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus.” 2014. Web. 07 Mar 2021.

Vancouver:

Francis Stuart SD. The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/152822.

Council of Science Editors:

Francis Stuart SD. The Pro-Antioxidant Role of Zinc Supplementation in Cadmium-Treated Choroid Plexus. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/152822

17. Morete, Nádia Patricia Amaral. Neuroinflammation and neurogenesis in the choroid plexus.

Degree: 2014, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5594

► Sendo parte integrante do sistema nervoso central, os plexos coroides (CP) são estruturas ramificadas, altamente vascularizadas, com uma camada de células epiteliais secretoras que projetam… (more)

▼ Sendo parte integrante do sistema nervoso central, os plexos coroides (CP) são estruturas ramificadas, altamente vascularizadas, com uma camada de células epiteliais secretoras que projetam numerosas vilosidades nos quatro ventrículos do cérebro. Os CP são constituídos por uma monocamada externa de células epiteliais coroidais (CPEC), cuja membrana apical está em contacto direto com o líquido cefalorraquidiano, enquanto que a membrana basal das CPEC se localiza sobre o estroma. O estroma é rodeado por células dendríticas, fibras de colagénio, fibroblastos e células B e T. Neste conjunto, encontram-se células imunitárias inatas cuja função é apresentar antigénios, moléculas que são capazes de iniciar resposta imune, o que sugere que o CP pode desempenhar um papel ativo na proteção imune do cérebro. Durante muito tempo, pensava-se que o cérebro era uma estrutura “imunologicamente privilegiada”, isto é, que o cérebro se encontrasse separado fisicamente do sistema imunitário periférico através da barreira hematoencefálica, não havendo trocas entre o cérebro e o restante organismo. A barreira hematoencefálica em conjunto com a barreira formada pelas CPEC protege o sistema nervoso central contra agentes patogénicos através das junções intercelulares que são compostas por proteínas de integridade de membrana. Estas proteínas permitem ao CP ter capacidade imunitária, uma vez que controlam a permeabilidade celular, impedindo os solutos de água de passar livremente, formando uma fronteira entre os domínios de membrana plasmática apicais e basolaterais, impedindo a passagem de proteínas entre os compartimentos da membrana. Não obstante, a permeabilidade da barreira pode ser influenciada por uma neuroinflamação permanente. A neuroinflamação é caracterizada pela ativação da microglia, stress oxidativo e expressão dos principais mediadores inflamatórios sendo um processo ativo de defesa contra vários insultos, lesões traumáticas e metabólicas, infeções e doenças neurodegenerativas tais como a doença de Alzheimer e de Parkinson. Outro “dogma” das neurociências durante muitos anos foi a não existência de neurogénese, isto é, as células do sistema nervoso não se regeneravam. Contudo, estudos recentes afirmam que o CP, quando sujeito a uma agressão, tem capacidade de formar novas células e estas por sua vez são capazes de se diferenciarem. Assim, neste trabalho propusemo-nos a avaliar o efeito de um insulto neuroinflamatório agudo provocado pelo lipopolissacarídeo (LPS), na neurogénese e na neuroinflamação do CP. Mais especificamente, a resposta inflamatória induzida pelo LPS no CP foi avaliada in vivo, ex vivo e in vitro através da análise de marcadores inflamatórios tais como CD11b, iNOS, expressão de TTR e produção de espécies reativas de oxigénio (ROS) e de óxido nítrico (NO). Avaliámos também o efeito do LPS na neurogénese in vivo e ex vivo através do estudo da presença de diferentes marcadores: NeuN, CD11b e o GFAP. Por fim, verificámos se o LPS teria algum efeito na integridade da membrana. A resposta inflamatória foi… Advisors/Committee Members: Santos, Cecília Reis Alves dos, Tomás, Joana.

Subjects/Keywords: Choroid Plexus; Neurogenesis; Neuroinflammation; Tight Junctions.; Domínio/Área Científica::Ciências Médicas::Ciências da Saúde

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morete, N. P. A. (2014). Neuroinflammation and neurogenesis in the choroid plexus. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5594

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Morete, Nádia Patricia Amaral. “Neuroinflammation and neurogenesis in the choroid plexus.” 2014. Thesis, RCAAP. Accessed March 07, 2021. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5594.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Morete, Nádia Patricia Amaral. “Neuroinflammation and neurogenesis in the choroid plexus.” 2014. Web. 07 Mar 2021.

Vancouver:

Morete NPA. Neuroinflammation and neurogenesis in the choroid plexus. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Mar 07]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5594.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Morete NPA. Neuroinflammation and neurogenesis in the choroid plexus. [Thesis]. RCAAP; 2014. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/5594

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

18. Kamaly-Asl, Ian. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.

Degree: Thesis (M.D.), 2011, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/molecular-genetic-patient-and-surgical-factors-involved-in-the-development-and-outcome-of-central-nervous-system-tumours(3e42d00e-b543-452e-bd71-ff53e194d944).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532199

► Prognostic factors come in a variety of forms and may be patient, tumour or environmental related. This thesis examines the interaction of prognostic factors for… (more)

Subjects/Keywords: 616.994; Brain Tumours; Prognosis; Vascular Endothelial Growth Factor; Astrocytoma; Choroid Plexus Carcinoma; Medulloblastoma; Ependymoma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kamaly-Asl, I. (2011). Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/molecular-genetic-patient-and-surgical-factors-involved-in-the-development-and-outcome-of-central-nervous-system-tumours(3e42d00e-b543-452e-bd71-ff53e194d944).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532199

Chicago Manual of Style (16^th Edition):

Kamaly-Asl, Ian. “Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.” 2011. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. https://www.research.manchester.ac.uk/portal/en/theses/molecular-genetic-patient-and-surgical-factors-involved-in-the-development-and-outcome-of-central-nervous-system-tumours(3e42d00e-b543-452e-bd71-ff53e194d944).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532199.

MLA Handbook (7^th Edition):

Kamaly-Asl, Ian. “Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours.” 2011. Web. 07 Mar 2021.

Vancouver:

Kamaly-Asl I. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Mar 07]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/molecular-genetic-patient-and-surgical-factors-involved-in-the-development-and-outcome-of-central-nervous-system-tumours(3e42d00e-b543-452e-bd71-ff53e194d944).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532199.

Council of Science Editors:

Kamaly-Asl I. Molecular, genetic, patient and surgical factors involved in the development and outcome of central nervous system tumours. [Doctoral Dissertation]. University of Manchester; 2011. Available from: https://www.research.manchester.ac.uk/portal/en/theses/molecular-genetic-patient-and-surgical-factors-involved-in-the-development-and-outcome-of-central-nervous-system-tumours(3e42d00e-b543-452e-bd71-ff53e194d944).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532199

University of Minnesota

19. Churchill, Caroline Catharine. The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance.

Degree: PhD, Experimental & Clinical Pharmacology, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/171058

► Opioid-based pharmacotherapy remains the most commonly prescribed treatment for patients suffering from neuropathic pain conditions. Although opioids are effective for treating neuropathic pain, when used… (more)

Subjects/Keywords: Adeno associated virus; Agmatine; Analgesic tolerance; Arginine decarboxylase; Choroid plexus; Experimental & clinical pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Churchill, C. C. (2014). The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/171058

Chicago Manual of Style (16^th Edition):

Churchill, Caroline Catharine. “The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance.” 2014. Doctoral Dissertation, University of Minnesota. Accessed March 07, 2021. http://hdl.handle.net/11299/171058.

MLA Handbook (7^th Edition):

Churchill, Caroline Catharine. “The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance.” 2014. Web. 07 Mar 2021.

Vancouver:

Churchill CC. The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11299/171058.

Council of Science Editors:

Churchill CC. The impact of gene transfer of arginine decarboxylase to the central nervous system on opioid analgesic tolerance. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/171058

University of Michigan

20. Teuscher, Nathan S. Peptide transporters in the brain: Role of PEPT2 in choroid plexus.

Degree: PhD, Pure Sciences, 2002, University of Michigan

URL: http://hdl.handle.net/2027.42/123077

► Peptide transporters have become increasingly important in drug design and phamacokinetic analysis over the past 10 years. These transporters are involved in the handling of… (more)

Subjects/Keywords: Brain; Choroid Plexus; Neuropeptides; Pept2; Peptide Transporters; Role

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teuscher, N. S. (2002). Peptide transporters in the brain: Role of PEPT2 in choroid plexus. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/123077

Chicago Manual of Style (16^th Edition):

Teuscher, Nathan S. “Peptide transporters in the brain: Role of PEPT2 in choroid plexus.” 2002. Doctoral Dissertation, University of Michigan. Accessed March 07, 2021. http://hdl.handle.net/2027.42/123077.

MLA Handbook (7^th Edition):

Teuscher, Nathan S. “Peptide transporters in the brain: Role of PEPT2 in choroid plexus.” 2002. Web. 07 Mar 2021.

Vancouver:

Teuscher NS. Peptide transporters in the brain: Role of PEPT2 in choroid plexus. [Internet] [Doctoral dissertation]. University of Michigan; 2002. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2027.42/123077.

Council of Science Editors:

Teuscher NS. Peptide transporters in the brain: Role of PEPT2 in choroid plexus. [Doctoral Dissertation]. University of Michigan; 2002. Available from: http://hdl.handle.net/2027.42/123077

University of Manchester

21. Hughes, Alexandra. Mechanisms of volume regulation in murine choroid plexus epithelial cells.

Degree: 2010, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:96675

►

The choroid plexuses are largely responsible for cerebrospinal fluid (CSF) secretion and therefore play a fundamental role in brain homeostasis. The membrane proteins involved in… (more)

▼

The choroid plexuses are largely responsible for cerebrospinal fluid (CSF) secretion and therefore play a fundamental role in brain homeostasis. The membrane proteins involved in CSF secretion are not fully known. Several electroneutral transporters have been identified by molecular methods in choroid plexus epithelial cells but there is a lack of functional data to support their expression making it impossible to elucidate their role in CSF secretion fully. The activity of many of these transporters can be observed in cell volume regulation. Thus, the main aim of the present study was to determine the ability of mammalian choroid plexus epithelial cells to regulate their volume in response to anisosmotic challenge and to investigate the transporters involved.Experiments were performed on cells isolated from the mouse fourth ventricle choroid plexus. Cells were isolated using a combination of manual perturbation, the enzyme dispase and a Ca2+ free incubation to disrupt tight junctions. Cell volume was measured using a video-imaging method. Cells used in this study were all of a similar morphology and had a mean volume of 0.71 pL.Cells exhibited a HCO3- dependent regulatory volume increase (RVI) in response to hypertonic challenge. Strong evidence is presented that the Na+/H+ exchanger (NHE1) and the Cl-/HCO3- exchanger (AE2) contribute to the RVI but the Na+K+2Cl- cotransporter (NKCC1) and the epithelial Na+ channel (ENaC) do not. Choroid plexus cells exhibit a HCO3- dependent regulatory volume decrease (RVD) in response to hypotonic challenge. The RVD was unaffected by DIOA (an inhibitor of KCC activity), the K+ channel inhibitors TEA+, Ba2+ or 4AP or the Cl- channel inhibitors DIDS or NPPB. However removal of extracellular Ca2+ completely abolished cell swelling in response to hypotonic challenge. This sensitivity of volume change to Ca2+ was specific to cell swelling as cell shrinkage in hypertonic artificial CSF was unaffected by removal of extracellular Ca2+.Thus functional evidence is presented to further elucidate the role of several proteins in the choroid plexus cell volume regulatory response to anisosmotic challenge.

The brain is surrounded by salty liquid which provides physical and chemical support and protection to the brain. This liquid, called cerebrospinal fluid, also fills ventricles within the brain where it is mainly produced by the choroid plexus tissues. The choroid plexus cells form a tight barrier which prevents cerebrospinal fluid and blood from mixing and thereby disrupting brain function. The components of cerebrospinal fluid are moved across this barrier by several proteins located in the cell membranes of the choroid plexus cells. To investigate the interactions between mouse choroid plexus cells and their surrounding environment, visible changes in cell volume were measured when cells were exposed to different bathing solutions. Choroid plexus cells are able to regulate their volume. When induced to shrink they activate mechanisms to re-swell to their approximate normal volume, this…

Advisors/Committee Members: Brown, Peter.

Subjects/Keywords: choroid plexus; volume regulation; murine epithelial cell isolation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hughes, A. (2010). Mechanisms of volume regulation in murine choroid plexus epithelial cells. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:96675

Chicago Manual of Style (16^th Edition):

Hughes, Alexandra. “Mechanisms of volume regulation in murine choroid plexus epithelial cells.” 2010. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:96675.

MLA Handbook (7^th Edition):

Hughes, Alexandra. “Mechanisms of volume regulation in murine choroid plexus epithelial cells.” 2010. Web. 07 Mar 2021.

Vancouver:

Hughes A. Mechanisms of volume regulation in murine choroid plexus epithelial cells. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2021 Mar 07]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:96675.

Council of Science Editors:

Hughes A. Mechanisms of volume regulation in murine choroid plexus epithelial cells. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:96675

Penn State University

22. Cozza, Eugene Michael. Host-conditioning strategies for adoptive T cell immunotherapy of cancer.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/26216

► Cancer immunotherapy is poised at the cutting edge of cancer research due to higher response rates and the potential for long-lasting cancer regressions. T cells… (more)

▼ Cancer immunotherapy is poised at the cutting edge of cancer research due to higher response rates and the potential for long-lasting cancer regressions. T cells are critical immune effectors that can identify and eliminate cancerous host cells. Adoptive T cell transfer (ACT) is an emerging immunotherapy in which the patient’s own T cells are isolated and optimized to target the tumor, expanded in culture, and re-infused into the cancer patient. To improve the survival and function of the re-infused T cells, patients are pre-conditioned with chemotherapy or whole-body irradiation (WBI) to deplete host immune cells prior to ACT. Use of host-conditioning regimens significantly improves response and cure rates, but the reasons for failure are unknown. Investigation of the underlying mechanisms of ACT therapy and host-conditioning regimens is necessary to improve the success and applicability of this promising immunotherapeutic approach. Here, we focus on the influence of WBI and agonist anti-CD40 antibody (which stimulates antigen-presenting cells to activate T cells) host-conditioning regimens on ACT therapy of established murine brain tumors. SV11 mice develop autochthonous tumors of the choroid plexus of the brain due to transgenic expression of the SV40 large Tumor antigen (T Ag) oncoprotein. Using T Ag-specific transgenic T cells, we investigate the underlying mechanisms of WBI and anti-CD40 that promote the in vivo accumulation and persistence of donor T cells during ACT-mediated tumor regression and protection from tumor recurrence. First, we show that WBI conditioning induces a prolonged window of opportunity during which ACT can be administered to mediate regression of established tumors. However, ACT early after WBI is required to achieve maximum survival benefit. Thus, the mechanisms promoting tumor regression and long-term survival are distinct. We also demonstrate a time-dependent reduction in early donor T cell accumulation when ACT is delayed after WBI that correlates with recovery from host lymphodepletion. Next, we dissect the local and systemic influences of WBI and show that irradiation conditioning can promote successful ACT-mediated tumor regression independently of local irradiation to the brain or tumor. Therefore, the systemic effects of WBI are critical determinants of ACT therapeutic success. Irradiation localized to the body was sufficient to promote long-term survival and donor T cell persistence in the brain. Local irradiation to the tumor site enhanced donor T cell accumulation in the tumor-draining lymph nodes and resulted in modest survival benefit but did not promote tumor regression. Finally, we show that anti-CD40 conditioning can promote ACT-mediated tumor regression in the absence of irradiation, but that only WBI conditioning promotes durable protection from tumor recurrence, demonstrating the differential efficacy of two clinically relevant immunotherapies. Protection from tumor recurrence was associated with the establishment of persistent donor T cells in the brain that were… Advisors/Committee Members: Todd Schell, Dissertation Advisor/Co-Advisor, Todd Schell, Committee Chair/Co-Chair, Aron Eliot Lukacher, Committee Member, Neil David Christensen, Committee Member, Jianxun Song, Committee Member, Edward Joseph Gunther, Special Member.

Subjects/Keywords: CD8+ T cell; Whole-body irradiation; CD40 agonist; choroid plexus tumor; SV40 T antigen transgenic mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cozza, E. M. (2015). Host-conditioning strategies for adoptive T cell immunotherapy of cancer. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/26216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cozza, Eugene Michael. “Host-conditioning strategies for adoptive T cell immunotherapy of cancer.” 2015. Thesis, Penn State University. Accessed March 07, 2021. https://submit-etda.libraries.psu.edu/catalog/26216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cozza, Eugene Michael. “Host-conditioning strategies for adoptive T cell immunotherapy of cancer.” 2015. Web. 07 Mar 2021.

Vancouver:

Cozza EM. Host-conditioning strategies for adoptive T cell immunotherapy of cancer. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Mar 07]. Available from: https://submit-etda.libraries.psu.edu/catalog/26216.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cozza EM. Host-conditioning strategies for adoptive T cell immunotherapy of cancer. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/26216

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Duarte, Ana Catarina Abreu. Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus.

Degree: 2014, RCAAP

URL: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6055

► The choroid plexus is a multifunctional tissue responsible for a wide range of homeostatic functions crucial to the central nervous system, including secretion of cerebrospinal… (more)

Subjects/Keywords: Amyloid-Beta; Bisphenol A; Blood-Cerebrospinal Fluid Barrier; Choroid Plexus; Tight Junctions; Transthyretin; Domínio/Área Científica::Ciências Médicas::Ciências da Saúde

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Duarte, A. C. A. (2014). Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus. (Thesis). RCAAP. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Duarte, Ana Catarina Abreu. “Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus.” 2014. Thesis, RCAAP. Accessed March 07, 2021. https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Duarte, Ana Catarina Abreu. “Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus.” 2014. Web. 07 Mar 2021.

Vancouver:

Duarte ACA. Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus. [Internet] [Thesis]. RCAAP; 2014. [cited 2021 Mar 07]. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6055.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Duarte ACA. Bisphenol A effects in transthyretin and barrier integrity markers in the choroid plexus. [Thesis]. RCAAP; 2014. Available from: https://www.rcaap.pt/detail.jsp?id=oai:ubibliorum.ubi.pt:10400.6/6055

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

24. Hanke Vela, Laura. Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-27247

► Aktuelle Forschungsergebnisse zur Multiplen Sklerose (MS) und dem dazugehörigen Tiermodell, Experimentelle autoimmune Enzephalomyelitis (EAE), unterstreichen die Rolle von Hirnschranken für den Beginn und die Aufrechterhaltung… (more)

▼ Aktuelle Forschungsergebnisse zur Multiplen Sklerose (MS) und dem dazugehörigen Tiermodell, Experimentelle autoimmune Enzephalomyelitis (EAE), unterstreichen die Rolle von Hirnschranken für den Beginn und die Aufrechterhaltung der Neuroinflammation. In den letzten Jahren rückte die Suche nach Visualisierungsmethoden beeinträchtigter Schranken als Methode zur frühen Diagnostik und zum Verständnis grundlegender pathophysiologischer Prozesse der Neuroinflammation in den Fokus. Gängige Bildgebungsmethoden, die Gadolinium-basierte Kontrastmittel benutzen, haben verschiedene Nachteile, da sie weder alle Aspekte dysfunktionaler Blut-Hirn-Schranken zeigen, noch eindeutige Klarheit über potentielle toxische Effekte wiederholter Kontrastmittelgaben besteht. Unsere Arbeitsgruppe hat sich in den letzten Jahren der Erforschung neuartiger Magnetresonanz (MR) - Bildgebungsmethoden gewidmet, die es erlauben, in vivo Untersuchungen mit histopathologischen und molekularbiologischen Einsichten zu vereinen. In der vorliegenden Arbeit nutzten wir eine neue Art von elektromagnetischen Nanopartikeln, die Europium-dotierten sehr kleinen Eisenoxid Partikel (engl. Europium-doped very small iron oxide particles = Eu-VSOP), um Störungen der Blut-Liquor-Schranke am Plexus choroideus (CP) zu visualisieren. Dafür führten wir, nach intravenöser Gabe von Eu-VSOP, zu verschiedenen Zeitpunkten der EAE MR-Aufnahmen durch und analysierten fluoreszenzmikroskopisch die dazugehörigen histopathologischen Schnitte. Unsere Ergebnisse zeigen, dass Eu-VSOP die in vivo Visualisierung von Hirnschrankenstörungen und neuroinflammatorischen Prozessen ermöglichen und erlauben, diese post mortem mit Veränderungen des CP zu verknüpfen. Unsere Forschungsgruppe hat in vorhergehenden Studien mit MR-Elastographie (MRE) in MS und EAE gezeigt, dass chronisch-entzündliche Erkrankungen des zentralen Nervensystems (ZNS) mit einem Verlust der Steifheit des Hirngewebes einhergehen. Im zweiten Teil der vorliegenden Studie führten wir nun MRE-Messungen in EAE-Mäusen durch, um mittels Veränderungen mechanischer Eigenschaften des Hirngewebes Störungen der Blut-Hirn-Schranke (BHS) zu demaskieren. Wir konnten zeigen, dass jene Hirnregionen, die stärker von der Neuroinflammation betroffen sind, besonders starke Verluste der Steifigkeit präsentieren und dass diese Veränderungen, anders als bei Verwendung gadoliniumhaltiger Kontrastmittel, mit dem klinischen Verlauf korrelieren. Weiterhin konnten wir beweisen, dass perivaskuläre Läsionen, in denen die BHS gestört ist, ein Remodeling mit Aggregaten des Extrazellulärmatrix-Proteins Fibronektin (Fn) durchlaufen, und dass die Genexpression dieses Proteoglykans mit MRE-Veränderungen korreliert. Damit stellt diese Studie eine Grundlage zur Visualisierung von Hirnschrankenstörungen als zentraler pathophysiologischer Prozess zur Entstehung neuroinflammatorischer Läsionen sowie zur Implementierung neuer MR-basierter Bildgebungsmethoden als Diagnosemittel der MS dar. Advisors/Committee Members: female (gender), N.N. (firstReferee), N.N. (furtherReferee).

Subjects/Keywords: Neuroinflammation; Brain-barrier; Magnetic Resonance Elastography; Experimental Autoimmune Encephalomyelitis; Europium-VSOP; Blood-brain-barrier; Choroid Plexus; ddc:610

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hanke Vela, L. (2020). Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-27247

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hanke Vela, Laura. “Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose.” 2020. Thesis, Freie Universität Berlin. Accessed March 07, 2021. http://dx.doi.org/10.17169/refubium-27247.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hanke Vela, Laura. “Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose.” 2020. Web. 07 Mar 2021.

Vancouver:

Hanke Vela L. Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Mar 07]. Available from: http://dx.doi.org/10.17169/refubium-27247.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hanke Vela L. Neue MR-Bildgebungsmethoden visualisieren Hirnschrankenstörungen im Mausmodell der Multiplen Sklerose. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-27247

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Schmitt, Charlotte. Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation.

Degree: Docteur es, Neurosciences, 2012, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2012LYO10003

►

Le système nerveux central est un site particulier vis-à-vis du système immunitaire, en raison de la présence de la barrière hémato-encéphalique et de la barrière… (more)

▼

Le système nerveux central est un site particulier vis-à-vis du système immunitaire, en raison de la présence de la barrière hémato-encéphalique et de la barrière sang-liquide céphalorachidien. Les plexus choroïdes ont été considérés comme une voie d’entrée de certains lymphocytes dans le système nerveux central. Et le liquide céphalo-rachidien a été considéré comme une voie préférentielle de circulation des cellules immune au cours de la surveillance neuro-immunitaire de l’ensemble des compartiments cérébraux, puisque le LCR circule des ventricules, aux espaces sous-arachnoïdiens ainsi qu’aux velum et citernes internes. L’implication du système plexus choroïdes-liquide céphalorachidien dans l’infiltration cellulaire et la distribution des différents effecteurs immuns a été évaluée. Premièrement, nous avons analysé la relation entre le LCR et la répartition des différentes cellules immune au sein du système nerveux central, dans deux modèles d’encéphalite autoimmune expérimentale, utilisé comme modèle de la sclérose en plaque. Deuxièmement, nous avons recherché les partenaires moléculaires pouvant être impliqués dans la mise en place d’une inflammation, tels que les molécules d’adhésion exprimés par l’épithélium choroïdien, et les chimiokines pouvant être sécrétées dans le liquide céphalorachidien. Nos résultats identifient les plexus choroïdes comme une source de chimiokines sécrétées dans le liquide céphalorachidien, ce dernier orchestrant la distribution des différents effecteurs immunitaire au cours de l’inflammation

The central nervous system is an immunologically specialized site, because of the blood-brain barrier and the blood-cerebrospinal fluid barrier. The choroid plexuses had been considered as a preferential site for the entry of lymphocytes into the CNS. And the cerebrospinal fluid has been considered as a preferential pathway of circulation for immune cells during physiological neuroimmune surveillance, in all cerebral compartments, as the cerebrospinal fluid circulates from the ventricles to the subarachnoid spaces as well as the velum and internal cisterns. We evaluate the involvement of the choroid plexus-cerebrospinal fluid system in the cerebral infiltration and distribution of immune cells in CNS inflammation. First we realized a time course analysis of the different type of immune cell association with the CSF-containing compartments in two experimental autoimmune encephalomyelitis, models of multiple sclerosis. Secondly, we analyzed the molecular partners that could be involved in CNS inflammation development, such as adhesion molecules expressed on the choroid plexus, and chemokines secreted into the cerebrospinal fluid. Results identified the choroid plexuses as a source of chemokines, released into the cerebrospinal fluid that orchestrates the immune cell invasion during CNS inflammation

Advisors/Committee Members: Ghersi-Egea, Jean-François (thesis director).

Subjects/Keywords: Encéphalomyélite auto-immune expérimentale; Plexus choroïdes; Liquide céphalorachidien; Chimiokines; Inflammation; Experimental autoimmune encephalomyelitis; Choroid plexuses; Cerebrospinal fluid; Chemokines; Inflammation; 616.83

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schmitt, C. (2012). Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2012LYO10003

Chicago Manual of Style (16^th Edition):

Schmitt, Charlotte. “Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation.” 2012. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed March 07, 2021. http://www.theses.fr/2012LYO10003.

MLA Handbook (7^th Edition):

Schmitt, Charlotte. “Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation.” 2012. Web. 07 Mar 2021.

Vancouver:

Schmitt C. Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2012. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2012LYO10003.

Council of Science Editors:

Schmitt C. Rôle du système plexus choroïde-liquide céphalorachidien dans la distribution des cellules immunes au sein du système nerveux central, exemple de l’encéphalomyélite auto-immune expérimentale : The choroid plexus-cerebrospinal fluid system are involved in the early infiltration of immune cells in central nervous system inflammation. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2012. Available from: http://www.theses.fr/2012LYO10003

26. Saudrais, Élodie. Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal.

Degree: Docteur es, Neurotoxicologie, 2019, Lyon

URL: http://www.theses.fr/2019LYSE1026

►

Plus de 50 % des handicaps neurodéveloppementaux sont dus à une exposition périnatale à des stress toxiques ou oxydants. Comprendre comment le cerveau est protégé… (more)

▼

Plus de 50 % des handicaps neurodéveloppementaux sont dus à une exposition périnatale à des stress toxiques ou oxydants. Comprendre comment le cerveau est protégé au cours du développement périnatal et pourquoi ses mécanismes de défense sont dépassés lorsque l’enfant est soumis à un stress important est donc crucial. La barrière sang – liquide céphalorachidien (LCR), localisée au niveau des plexus choroïdes, présente une capacité de détoxification élevée et pourrait donc avoir un rôle prépondérant dans la protection du cerveau au stade périnatal. Nous avons étudié la capacité de plusieurs enzymes choroïdiennes à protéger l'environnement liquidien cérébral pendant la période postnatale chez le rat, et évalué si leurs activités pouvaient être induites par la voie du nuclear factor erythroid-2-related factor 2 (Nrf2). Le facteur Nrf2 peut en effet moduler l’expression de différents gènes codant pour des enzymes de détoxification. Nous avons montré que les glutathion transférases (Gst) et les glutathion peroxydases (Gpx), intervenant respectivement dans l’inactivation des molécules toxiques et dans la régulation du stress oxydant, présentaient des activités choroïdiennes élevées pendant la période postnatale, et avons caractérisé fonctionnellement leur capacités de neuroprotection. Le traitement des ratons avec du diméthylfumarate (DMF), inducteur de la voie Nrf2, induit la migration nucléaire de Nrf2, augmente l’activité choroïdienne Gst, et réduit de 40 % le passage cérébral de toxiques substrats des Gst. Ces données montrent la capacité neuroprotectrice précoce des plexus choroïdes, et indique qu’elle peut être induite pharmacologiquement

More than 50 % of intellectual or sensory-motor deficits in children are due to perinatal exposure to oxidative stress or toxicants. Understanding brain protection mechanisms during development is crucial to design therapeutic strategies to address these disabilitating disorders. The choroid plexuses, forming an interface between the blood and the cerebrospinal fluid (CSF), have a high detoxifying capacity, suggesting their involvement in neuroprotection. The nuclear factor erythroid-2-related factor 2 (Nrf2) pathway can modulate the expression of several genes encoding for antioxidant proteins and detoxifying enzymes. We studied the ability of several choroidal enzyme families to protect the brain fluid environment during the postnatal period in rat and explored whether this protection can be enhanced by Nrf2 pathway. We focused on glutathione transferases (Gsts), which conjugate toxic compounds to glutathione, and glutathione peroxidases (Gpxs), which detoxify reactive oxygen species. Gst and Gpx specific activities were high during the postnatal period in choroid plexuses compared to the cerebral cortex, and their neuroprotective functions were efficient. The Nrf2 factor is expressed in choroid plexuses during the perinatal period. Treatment of rat pups with Nrf2 activator dimethylfumarate induced Nrf2 nuclear translocation and increased Gst activities in choroid plexus…

Advisors/Committee Members: Ghersi-Egea, Jean-François (thesis director), Strazielle, Nathalie (thesis director).

Subjects/Keywords: Plexus choroïdes; Glutathion transférases; Glutathion peroxydase; Liquide céphalorachidien; Nrf2; Diméthylfumarate; Choroid plexuses; Glutathione transferases; Glutathione peroxidases; Cerebrospinal fluid; Nrf2; Dimethylfumarate; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saudrais, E. (2019). Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal. (Doctoral Dissertation). Lyon. Retrieved from http://www.theses.fr/2019LYSE1026

Chicago Manual of Style (16^th Edition):

Saudrais, Élodie. “Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal.” 2019. Doctoral Dissertation, Lyon. Accessed March 07, 2021. http://www.theses.fr/2019LYSE1026.

MLA Handbook (7^th Edition):

Saudrais, Élodie. “Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal.” 2019. Web. 07 Mar 2021.

Vancouver:

Saudrais E. Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal. [Internet] [Doctoral dissertation]. Lyon; 2019. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2019LYSE1026.

Council of Science Editors:

Saudrais E. Mécanismes de neuroprotection liés au glutathion dans la barrière sang - liquide céphalorachidien choroïdienne au cours du développement périnatal : Mécanismes de neuroprotection liés au glutathion dans la barrière sang-liquide céphalorachidien choroïdienne au cours du développement périnatal. [Doctoral Dissertation]. Lyon; 2019. Available from: http://www.theses.fr/2019LYSE1026

University of Manchester

27. Hughes, Alexandra. Mechanisms of volume regulation in murine choroid plexus epithelial cells.

Degree: PhD, 2010, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/mechanisms-of-volume-regulation-in-murine-choroid-plexus-epithelial-cells(66cb068e-0e38-4773-83ca-a7867aaff66c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529212

► The choroid plexuses are largely responsible for cerebrospinal fluid (CSF) secretion and therefore play a fundamental role in brain homeostasis. The membrane proteins involved in… (more)

▼ The choroid plexuses are largely responsible for cerebrospinal fluid (CSF) secretion and therefore play a fundamental role in brain homeostasis. The membrane proteins involved in CSF secretion are not fully known. Several electroneutral transporters have been identified by molecular methods in choroid plexus epithelial cells but there is a lack of functional data to support their expression making it impossible to elucidate their role in CSF secretion fully. The activity of many of these transporters can be observed in cell volume regulation. Thus, the main aim of the present study was to determine the ability of mammalian choroid plexus epithelial cells to regulate their volume in response to anisosmotic challenge and to investigate the transporters involved.Experiments were performed on cells isolated from the mouse fourth ventricle choroid plexus. Cells were isolated using a combination of manual perturbation, the enzyme dispase and a Ca2+ free incubation to disrupt tight junctions. Cell volume was measured using a video-imaging method. Cells used in this study were all of a similar morphology and had a mean volume of 0.71 pL.Cells exhibited a HCO3- dependent regulatory volume increase (RVI) in response to hypertonic challenge. Strong evidence is presented that the Na+/H+ exchanger (NHE1) and the Cl-/HCO3- exchanger (AE2) contribute to the RVI but the Na+K+2Cl- cotransporter (NKCC1) and the epithelial Na+ channel (ENaC) do not. Choroid plexus cells exhibit a HCO3- dependent regulatory volume decrease (RVD) in response to hypotonic challenge. The RVD was unaffected by DIOA (an inhibitor of KCC activity), the K+ channel inhibitors TEA+, Ba2+ or 4AP or the Cl- channel inhibitors DIDS or NPPB. However removal of extracellular Ca2+ completely abolished cell swelling in response to hypotonic challenge. This sensitivity of volume change to Ca2+ was specific to cell swelling as cell shrinkage in hypertonic artificial CSF was unaffected by removal of extracellular Ca2+.Thus functional evidence is presented to further elucidate the role of several proteins in the choroid plexus cell volume regulatory response to anisosmotic challenge.

Subjects/Keywords: 573.8; choroid plexus; volume regulation; murine epithelial cell isolation

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APA (6^th Edition):

Hughes, A. (2010). Mechanisms of volume regulation in murine choroid plexus epithelial cells. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/mechanisms-of-volume-regulation-in-murine-choroid-plexus-epithelial-cells(66cb068e-0e38-4773-83ca-a7867aaff66c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529212

Chicago Manual of Style (16^th Edition):

Hughes, Alexandra. “Mechanisms of volume regulation in murine choroid plexus epithelial cells.” 2010. Doctoral Dissertation, University of Manchester. Accessed March 07, 2021. https://www.research.manchester.ac.uk/portal/en/theses/mechanisms-of-volume-regulation-in-murine-choroid-plexus-epithelial-cells(66cb068e-0e38-4773-83ca-a7867aaff66c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529212.

MLA Handbook (7^th Edition):

Hughes, Alexandra. “Mechanisms of volume regulation in murine choroid plexus epithelial cells.” 2010. Web. 07 Mar 2021.

Vancouver:

Hughes A. Mechanisms of volume regulation in murine choroid plexus epithelial cells. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2021 Mar 07]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/mechanisms-of-volume-regulation-in-murine-choroid-plexus-epithelial-cells(66cb068e-0e38-4773-83ca-a7867aaff66c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529212.

Council of Science Editors:

Hughes A. Mechanisms of volume regulation in murine choroid plexus epithelial cells. [Doctoral Dissertation]. University of Manchester; 2010. Available from: https://www.research.manchester.ac.uk/portal/en/theses/mechanisms-of-volume-regulation-in-murine-choroid-plexus-epithelial-cells(66cb068e-0e38-4773-83ca-a7867aaff66c).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529212

28. Mottahedin, Amin. Developing brain and systemic inflammation: a "Toll-like" link with consequences.

Degree: 2017, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/51891

► The developing brain is vulnerable to external insults, and perinatal brain injury (PBI) is a major cause of life-long neurological syndromes such as cerebral palsy.… (more)

▼ The developing brain is vulnerable to external insults, and perinatal brain injury (PBI) is a major cause of life-long neurological syndromes such as cerebral palsy. Currently, no pharmaceutical intervention is available. Hypoxia/ischemia (HI), infections and inflammation are implicated in the pathogenesis of PBI. However, the crosstalk between these etiologies is not fully understood. Toll-like receptors (TLR) 3 and TLR2 are responsible for sensing viral and bacterial infections and initiating the inflammatory response. The aim of this thesis was to investigate the effect of systemic inflammation induced by activation of these TLRs on neonatal HI brain injury. We demonstrate that intraperitoneal administration of TLR3 and TLR2 ligands (PolyI:C and P3C, respectively) prior to HI increases the brain injury in neonatal mice. PolyI:C and P3C induced neuroinflammation and altered microglial phenotype as assessed by RT-qPCR, multiplex cytokine assay or flow cytometry. PolyI:C also upregulated the pro-apoptotic gene, Fasl, expression and reduced activation of pro-survival signaling molecule Akt. On the other hand, P3C suppressed mitochondrial respiration, a major mechanism of cellular energy production. P3C, unlike other TLR agonists, induced marked infiltration of leukocytes to the cerebral spinal fluid and brain of neonatal mice and rats. Confocal microscopy, Cre recombinase-mediated gene targeting and in vitro cell transmigra-tion assay revealed the choroid plexus as a site of leukocyte entry. RNA sequencing of the choroid plexus followed by transcriptome cluster analysis and Ingenuity Pathway Analysis revealed potential mechanisms of leukocyte infiltration, including a specific chemotaxis signature and cytoskeleton-related pathways. Finally, we show that N-acetylcysteine treatment inhibits TLR2-mediated leukocyte trafficking in vivo and in vitro. To conclude, this thesis describe a TLR-mediated link between systemic inflammation and developing brain with detrimental consequences on HI brain injury, suggesting potential novel therapeutic strategies.

Subjects/Keywords: developing brain; neonatal brain injury; hypoxia-ischemia; inflammation; infection; Toll-like receptor; choroid plexus; neuroimmunology; Mitochondria

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mottahedin, A. (2017). Developing brain and systemic inflammation: a "Toll-like" link with consequences. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/51891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mottahedin, Amin. “Developing brain and systemic inflammation: a "Toll-like" link with consequences.” 2017. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed March 07, 2021. http://hdl.handle.net/2077/51891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mottahedin, Amin. “Developing brain and systemic inflammation: a "Toll-like" link with consequences.” 2017. Web. 07 Mar 2021.

Vancouver:

Mottahedin A. Developing brain and systemic inflammation: a "Toll-like" link with consequences. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2077/51891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mottahedin A. Developing brain and systemic inflammation: a "Toll-like" link with consequences. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2017. Available from: http://hdl.handle.net/2077/51891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Helton, Danielle Lee. Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice.

Degree: PhD, Biomedical Sciences, 2013, University of Tennessee Health Science Center

URL: https://dc.uthsc.edu/dissertations/342

► The lysosomal sialidase Neuraminidase-1 (Neu1) initiates the hydrolysis of sialoglycoconjugates by cleaving their terminal sialic acid residues. Neu1 creates a complex with the carboxypeptidase… (more)

Subjects/Keywords: choroid plexus; lysosomal storage disorders; lysosomes; sialidosis; tight junctions; Medical Sciences; Medicine and Health Sciences; Neurosciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Helton, D. L. (2013). Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice. (Doctoral Dissertation). University of Tennessee Health Science Center. Retrieved from https://dc.uthsc.edu/dissertations/342

Chicago Manual of Style (16^th Edition):

Helton, Danielle Lee. “Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice.” 2013. Doctoral Dissertation, University of Tennessee Health Science Center. Accessed March 07, 2021. https://dc.uthsc.edu/dissertations/342.

MLA Handbook (7^th Edition):

Helton, Danielle Lee. “Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice.” 2013. Web. 07 Mar 2021.

Vancouver:

Helton DL. Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice. [Internet] [Doctoral dissertation]. University of Tennessee Health Science Center; 2013. [cited 2021 Mar 07]. Available from: https://dc.uthsc.edu/dissertations/342.

Council of Science Editors:

Helton DL. Developmental Characterization of the Choroid Plexus in Sialidosis (Neu1 Deficient) Mice. [Doctoral Dissertation]. University of Tennessee Health Science Center; 2013. Available from: https://dc.uthsc.edu/dissertations/342

30. Henson, Hannah Elizabeth. Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish.

Degree: PhD, Biomedical Sciences, 2014, University of Tennessee Health Science Center

URL: https://dc.uthsc.edu/dissertations/340

► The choroid plexus (CP) is an epithelial based structure localized within the brain ventricles and functions as the blood-cerebrospinal fluid barrier (BCSFB). Under normal… (more)

▼ The choroid plexus (CP) is an epithelial based structure localized within the brain ventricles and functions as the blood-cerebrospinal fluid barrier (BCSFB). Under normal conditions, the CP is responsible for generating the cerebrospinal fluid (CSF) and regulating its components. Abnormal CP function has been associated with neurodegenerative diseases, tumor formation in CP epithelia (CPe), and hydrocephalus. Despite the significant role of the CP in these disorders, little research has been done to characterize its functional properties and genetically dissect the pathways involved in normal CP development and disease. For this study, we have utilized zebrafish, (Danio rerio), as a model system to better understand the genetic components of CP development. Their transparent nature and rapid ex utero development provide mechanisms to visualize CP formation that is not possible in other model systems. By utilizing an enhancer trap line, Et(cp:EGFP) sj2 that expresses the enhanced green fluorescent protein (EGFP) in CPe, we have demonstrated that the zebrafish CP possesses barrier properties such as tight junctions, transporter activity, and regulating central nervous system (CNS) homeostasis. After validating that the zebrafish CP is similar to higher vertebrates, we have initiated a genetic screen to answer questions such as: 1) what genes are needed for CP development, function, and maintenance, 2) what are the signaling pathways involved, and 3) how do these pathways interact with one another to form the BCSFB? This study is the first to employ an unbiased approach using a forward genetic screen to genetically dissect the CP and identify genes essential for its formation and function. As a result of this genetic analysis, we have confirmed 10 mutant lines with CP abnormalities. The mutants generated in this study will be used in future investigations to elucidate specific genes and signaling pathways essential for CP development, function, and maintenance and will provide a better understanding of how genetic mutations contribute to CP- related diseases. We have also mapped the gene for one mutant line, cp27.5, to Chromosome 21. Using a combination of classical positional cloning and whole-exome sequencing (WES), we identified the mutated gene as squamous cell carcinoma antigen recognized by T cells (sart1). sart1 plays a role in assembling the spliceosome, a multi-ribonucleoprotein complex essential for processing pre-mRNA. By characterizing cp27.5 mutants, we identified a number of proteins with altered expression levels and patterning primarily within the brain and eye. Using RNA-Seq analysis, we also identified multiple genes up-regulated or down-regulated due to the sart1 mutation. While sart1 itself was up-regulated, we also identified increased expression of genes involved in apoptosis such as tp53 and mdm2. A loss of photoreceptors and lack of retinal lamination in mutants is related to a down-regulation of vision-related genes such as phosphodiesterase 6H, (pde6h) and opsin-1, short-wave-sensitive 1… Advisors/Committee Members: Michael R. Taylor, Ph.D..

Subjects/Keywords: choroid plexus; forward genetic screen; Sart1; zebrafish; Medical Cell Biology; Medical Sciences; Medicine and Health Sciences

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Henson, H. E. (2014). Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish. (Doctoral Dissertation). University of Tennessee Health Science Center. Retrieved from https://dc.uthsc.edu/dissertations/340

Chicago Manual of Style (16^th Edition):

Henson, Hannah Elizabeth. “Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish.” 2014. Doctoral Dissertation, University of Tennessee Health Science Center. Accessed March 07, 2021. https://dc.uthsc.edu/dissertations/340.

MLA Handbook (7^th Edition):

Henson, Hannah Elizabeth. “Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish.” 2014. Web. 07 Mar 2021.

Vancouver:

Henson HE. Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish. [Internet] [Doctoral dissertation]. University of Tennessee Health Science Center; 2014. [cited 2021 Mar 07]. Available from: https://dc.uthsc.edu/dissertations/340.

Council of Science Editors:

Henson HE. Functional and Genetic Analysis of Choroid Plexus Development in Zebrafish. [Doctoral Dissertation]. University of Tennessee Health Science Center; 2014. Available from: https://dc.uthsc.edu/dissertations/340

Open Access Theses and Dissertations