subject:(cholinergic)

University of Gothenburg / Göteborgs Universitet

1. Strömbom, Ulf, 1945-. On the functional role of pre- and postsynaptic atecholamine receptors in brain.

Degree: 1975, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/14347

Subjects/Keywords: Receptors; cholinergic

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Strömbom, Ulf, 1. (1975). On the functional role of pre- and postsynaptic atecholamine receptors in brain. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/14347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Strömbom, Ulf, 1945-. “On the functional role of pre- and postsynaptic atecholamine receptors in brain.” 1975. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed January 19, 2021. http://hdl.handle.net/2077/14347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Strömbom, Ulf, 1945-. “On the functional role of pre- and postsynaptic atecholamine receptors in brain.” 1975. Web. 19 Jan 2021.

Vancouver:

Strömbom, Ulf 1. On the functional role of pre- and postsynaptic atecholamine receptors in brain. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 1975. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2077/14347.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Strömbom, Ulf 1. On the functional role of pre- and postsynaptic atecholamine receptors in brain. [Thesis]. University of Gothenburg / Göteborgs Universitet; 1975. Available from: http://hdl.handle.net/2077/14347

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

2. James, Nicholas. Cholinergic modulation of auditory and prefrontal cortical interactions.

Degree: PhD, Neuroscience, 2016, Boston University

URL: http://hdl.handle.net/2144/14605

► Much of the previous work investigating the influence of cholinergic tone on cortical circuits has emphasized global states of arousal and local circuit dynamics; however… (more)

▼ Much of the previous work investigating the influence of cholinergic tone on cortical circuits has emphasized global states of arousal and local circuit dynamics; however the cholinergic system is well-suited to coordinate large-scale cortical interactions due to its diffuse cortically projecting arborization and diverse influence on the various cell types within the cortical microcircuit. In this thesis I examined the function of cortical cholinergic tone in supporting long-range cortical interactions, feed-forward sensory signaling, and active processing of behaviorally relevant stimuli. I utilized optogenetic stimulation and silencing of the cholinergic nucleus basalis while recording from the auditory-prefrontal cortical circuit as well as performing local drug infusions in awake mice. I demonstrate that prefrontal cortex actively responds to cortico-cortical sensory input in animals passively presented with acoustic stimuli and that muscarinic receptor binding within auditory cortex is essential for feedforward pathways from auditory cortex to transmit sensory related neural signals. Specifically, muscarinic antagonists applied to the auditory cortex disrupt sensory signaling within auditory cortex as well as bottom up signaling to prefrontal cortex. Furthermore, muscarinic antagonists attenuated the influence of cortical cholinergic release on recording channels closest to drug infusion, confirming the efficacy of muscarinic antagonism, and demonstrating that aspects of cholinergic modulation are locally generated within cortical circuits, while others are globally generated in large networks. In task performing animals, I observed that optogenetic silencing of cholinergic nucleus basalis neurons attenuates the magnitude of prefrontal cortex alpha power following correct behavioral choice and that alpha in prefrontal and auditory cortical local field potentials are actively involved in behavioral learning during extinction, suggesting that cholinergic tone is involved in maintaining and updating the value of stimuli across behavioral trials. In summary, my thesis supports a model where endogenous cholinergic signaling is an essential component of normal auditory processing during low attentive states, contributes to circuit activation through local and large network mechanisms, and supports essential cortical dynamics that contribute to active behavioral processing of stimuli.

Subjects/Keywords: Neurosciences; Cholinergic; Attention; Corticocortical; Learning

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

James, N. (2016). Cholinergic modulation of auditory and prefrontal cortical interactions. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/14605

Chicago Manual of Style (16^th Edition):

James, Nicholas. “Cholinergic modulation of auditory and prefrontal cortical interactions.” 2016. Doctoral Dissertation, Boston University. Accessed January 19, 2021. http://hdl.handle.net/2144/14605.

MLA Handbook (7^th Edition):

James, Nicholas. “Cholinergic modulation of auditory and prefrontal cortical interactions.” 2016. Web. 19 Jan 2021.

Vancouver:

James N. Cholinergic modulation of auditory and prefrontal cortical interactions. [Internet] [Doctoral dissertation]. Boston University; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2144/14605.

Council of Science Editors:

James N. Cholinergic modulation of auditory and prefrontal cortical interactions. [Doctoral Dissertation]. Boston University; 2016. Available from: http://hdl.handle.net/2144/14605

University of Sydney

3. Morse, Ashleigh. The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell .

Degree: 2016, University of Sydney

URL: http://hdl.handle.net/2123/16289

► In the service of their basic needs and desires, animals and humans can use information from their environment to guide their choice between actions. In… (more)

▼ In the service of their basic needs and desires, animals and humans can use information from their environment to guide their choice between actions. In the laboratory, the ability for reward-predictive cues to control action selection is studied through outcome-specific Pavlovian-instrumental transfer (PIT-S), in which a stimulus associated with a particular outcomes biases choice between actions towards the response that earned that same outcome. This thesis investigates the determinants of PIT-S within the nucleus accumbens shell (NAc-S), which is selectively recruited to mediate PIT-S, and is not involved in encoding Pavlovian or instrumental associations. Previous work indicated that delta-opioid receptor (DOR) accumulation at the membrane of cholinergic interneurons (CIN-m) in the NAc-S is triggered during Pavlovian learning, and is positively correlated with PIT-S performance. We took three approaches to investigating the role of DOR accumulation: behavioural and pharmacological manipulation of established receptor accumulation, and chemogenetic manipulation of CINs and the afferents that likely utilize DOR accumulation within the NAc-S to mediate PIT-S. Manipulations of the predictive status of Pavlovian cues that abolished PIT-S failed to reverse established DOR accumulation, suggesting that a region that encodes this information controls the use of DOR accumulation to drive PIT-S. Specific pharmacological internalisation of DOR transiently reduced receptor expression on CIN-m in the NAc-S, indicating that CINs have `memory' for DOR accumulation. PIT-S performance was impaired during the period of DOR reduction, indicating that DOR accumulation on NAc-S CIN-m is necessary, but not sufficient, for PIT-S expression. Inactivation of CINs and BLA terminals within the NAc-S impaired and attenuated PIT-S, respectively. Both contralateral and ipsilateral BLA terminal disconnection from CINs in the NAc-S impaired PIT-S performance, despite ipsilateral BLA-NAc-S disconnection failing to affect PIT-S in previous studies, which complicates our ability to interpret this finding. These findings, considered together, suggest that DOR accumulation on CIN-m is necessary, but not sufficient, for PIT-S, and that a functional circuit between BLA terminals and CINs mediates PIT-S, via DOR accumulation on CIN-m.

Subjects/Keywords: Striatum; Accumbens; Pavlovian; Cholinergic

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morse, A. (2016). The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/16289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Morse, Ashleigh. “The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell .” 2016. Thesis, University of Sydney. Accessed January 19, 2021. http://hdl.handle.net/2123/16289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Morse, Ashleigh. “The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell .” 2016. Web. 19 Jan 2021.

Vancouver:

Morse A. The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell . [Internet] [Thesis]. University of Sydney; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2123/16289.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Morse A. The Determinants of Specific Pavlovian-Instrumental Transfer in the Nucleus Accumbens Shell . [Thesis]. University of Sydney; 2016. Available from: http://hdl.handle.net/2123/16289

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Univerzitet u Beogradu

4. Petrović, Jelena M., 1981-. Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova.

Degree: Biološki fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:11144/bdef:Content/get

Neuronauke - Neurofiziologija sa biofizikom / Neuroscience - Neurophysiology and Biophysics

Alchajmerova bolest (AB) i Parkinsonova bolest (PB) su najčešće neurodegenerativne bolesti starenja...

Advisors/Committee Members: Šaponjić, Jasna.

Subjects/Keywords: sllep; elektroencepfalography (EEG; electromyography(EMG); cholinergic neurons

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Petrović, Jelena M., 1. (2016). Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:11144/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Petrović, Jelena M., 1981-. “Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova.” 2016. Thesis, Univerzitet u Beogradu. Accessed January 19, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:11144/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Petrović, Jelena M., 1981-. “Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova.” 2016. Web. 19 Jan 2021.

Vancouver:

Petrović, Jelena M. 1. Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Jan 19]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11144/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Petrović, Jelena M. 1. Spavanje i elektroencefalografski ritmovi kao indikatori poremećaja funkcionalno različitih holinergičkih inervacija velikog mozga pacova. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:11144/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Rodway, Paul. The effects of smoking on aspects of visual attention.

Degree: PhD, 1996, University of Sussex

URL: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360538

Subjects/Keywords: 150; Nicotine; Cholinergic

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rodway, P. (1996). The effects of smoking on aspects of visual attention. (Doctoral Dissertation). University of Sussex. Retrieved from https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360538

Chicago Manual of Style (16^th Edition):

Rodway, Paul. “The effects of smoking on aspects of visual attention.” 1996. Doctoral Dissertation, University of Sussex. Accessed January 19, 2021. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360538.

MLA Handbook (7^th Edition):

Rodway, Paul. “The effects of smoking on aspects of visual attention.” 1996. Web. 19 Jan 2021.

Vancouver:

Rodway P. The effects of smoking on aspects of visual attention. [Internet] [Doctoral dissertation]. University of Sussex; 1996. [cited 2021 Jan 19]. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360538.

Council of Science Editors:

Rodway P. The effects of smoking on aspects of visual attention. [Doctoral Dissertation]. University of Sussex; 1996. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360538

Freie Universität Berlin

6. Altrichter, Sabine. Diagnosis and therapy of cholinergic urticaria.

Degree: 2020, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-28417

► Die cholinergische Urtikaria (CholU) ist eine der häufigsten Formen der chronischen induzierbaren Urtikaria. Bei dieser Erkrankung, die am häufigsten bei jungen Erwachsenen auftritt, kommt es… (more)

▼ Die cholinergische Urtikaria (CholU) ist eine der häufigsten Formen der chronischen induzierbaren Urtikaria. Bei dieser Erkrankung, die am häufigsten bei jungen Erwachsenen auftritt, kommt es nach körperlicher Anstrengung oder passiver Erwärmung zum Auftreten von multiplen kleinsten Quaddeln und z.T. starkem Juckreiz. Obwohl diese Erkrankung schon lange bekannt und nicht selten ist, gab es nur wenige Bemühungen bisher dieses Krankheitsbild zu entschlüsseln und wirksame Therapien für diese Patienten zu entwickeln. Ein Grund dafür könnte sein, dass die Erkrankung aufgrund der Flüchtigkeit und z.T. subjektiven Komponente der Symptome nur schwierig zu fassen ist. Etablierte Provokationsmethoden erlauben zwar eine Diagnosesicherung aber keine Schweregradeinteilung. Ein Großteil der hier vorgestellten Arbeiten fokussierte sich daher darauf diese Erkrankung „untersuchbar“ zu machen. Die standardisierte Puls-kontrollierte Ergometrie (sPCE) erlaubt nun erstmalig nicht nur eine Diagnosesicherung, sondern auch eine vergleichbare Anstrengung unabhängig vom Trainingszustand der Patienten. Der entwickelte cholinergische Urtikaria-Aktivitätsscore (CholUAS) wiederum erlaubt das Beschwerdemonitoring der Patienten in deren täglichen Setting und bezieht die Triggersituationen in die Berechnung mit ein. Zur Vervollständigung des Bildes der Patienten wurde ein krankheitsspezifisches Lebensqualitätsinstrument (CholU-QoL) entwickelt und validiert, um alle Aspekte der Patienten in Studien nachvollziehen zu können. Die weiteren hier vorgestellten Studien fokussieren sich auf die klinische Charakterisierung der CholU Patienten. Dies ist deswegen so wichtig, weil mehrere z.T. noch inkomplett verstandene pathophysiologische Ursachen für die CholU propagiert werden, für die unterschiedliche Behandlungsansätze vorstellbar sind. In den vorgestellten Studien wurden auch neue Patientenkohorten mit spezifischen Aspekten entdeckt, die möglicherweise auch unterschiedliches Therapieansprechen erklären können. Die bisherigen limitierten therapeutischen Studien, auch die hier vorgestellte Studie zur Hochdosis-Antihistaminikatherapie, zeigten jeweils insgesamt unzufriedenstellendes Ansprechen, bei zumindest einem Teil der Patienten. Auch eine „off-label“ Therapie mit Omalizumab (anti-IgE) zeigte nicht bei allen Patienten ein ansprechen. Weitere Studien, die all diese Aspekte mit einbeziehen und unter Verwendung der entwickelten diagnostischen Instrumente, sollten helfen die Therapie der Patienten in Zukunft zu verbessern. Advisors/Committee Members: female (gender), Buhl, Timo (firstReferee), Pfützner, Wolfgang (furtherReferee).

Subjects/Keywords: therapy; diagnosis; cholinergic urticaria; ddc:610

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Altrichter, S. (2020). Diagnosis and therapy of cholinergic urticaria. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-28417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Altrichter, Sabine. “Diagnosis and therapy of cholinergic urticaria.” 2020. Thesis, Freie Universität Berlin. Accessed January 19, 2021. http://dx.doi.org/10.17169/refubium-28417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Altrichter, Sabine. “Diagnosis and therapy of cholinergic urticaria.” 2020. Web. 19 Jan 2021.

Vancouver:

Altrichter S. Diagnosis and therapy of cholinergic urticaria. [Internet] [Thesis]. Freie Universität Berlin; 2020. [cited 2021 Jan 19]. Available from: http://dx.doi.org/10.17169/refubium-28417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Altrichter S. Diagnosis and therapy of cholinergic urticaria. [Thesis]. Freie Universität Berlin; 2020. Available from: http://dx.doi.org/10.17169/refubium-28417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne

7. Thomas, Natalie Paige. Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism.

Degree: 2016, University of Melbourne

URL: http://hdl.handle.net/11343/58776

► Background. A crucial step towards understanding the aetiology of schizophrenia (SCZ), and developing more effective diagnostic methods and treatments is to identify and characterise potential… (more)

▼ Background. A crucial step towards understanding the aetiology of schizophrenia (SCZ), and developing more effective diagnostic methods and treatments is to identify and characterise potential subgroups within the diagnosis. There is growing evidence to support the existence of biologically discrete subgroups; one such demonstration is a subgroup denoted muscarinic receptor deficient schizophrenia (MRDS). Making up 25% of the SCZ population, these subjects are separated due to their marked loss (75%) in [3H] pirenzepine binding (cholinergic muscarinic M1 receptor preferring ligand), measured in the dorsolateral prefrontal cortex (DLPFC) in post-mortem brain tissue. Having established the SCZMRDS subgroup in our laboratory, we have the ability to investigate the pathophysiology that presents within this discrete subgroup. Study 1. Previous reports suggest lower [3H] pirenzepine and [3H] AF-DX 384 (muscarinic M2/ M4 receptor preferring ligand) binding in the caudate putamen (C.Pu) in SCZ subjects. However, it is not known if SCZMRDS subjects drive this decreased binding in the C.Pu, as they do in cortical regions. Therefore, to better understand the changes in muscarinic receptors in the C.Pu from SCZ subjects, and to delineate whether the SCZMRDS subgroup was identifiable in subcortical regions, we measured [3H] pirenzepine and [3H] AF-DX 384, and [3H] 4’DAMP binding in C.Pu from 40 SCZ subjects, 20 of which were SCZMRDS, 20 that were MRDSNON-MRDS, and 20 non-psychiatric controls (CTRL). These measures gave good estimation of muscarinic M1 receptor (CHRM1), CHRM2/4, and CHRM3 receptor levels, respectively. The level of [3H] pirenzepine binding was significantly lower in the C.Pu from subjects with SCZ when compared to CTRL (p < 0.0007). This was driven by the SCZMRDS group (p < 0.0001 relative to CTRL). The levels of [3H] AF-DX 384 binding was significantly lower in the C.Pu from SCZ subjects compared to CTRL (p < 0.0001); this was demonstrated more strongly in SCZMRDS subjects (p < 0.0001) than SCZNON-MRDS subjects (p < 0.001) when compared to CTRL subjects. No significant differences in [3H] 4’DAMP measures were shown. Collectively, the results demonstrated that the SCZMRDS subgroup had significantly different CHRM density profiles within the striatum, when compared to SCZNON-MRDS and CTRLS. These results reinforce the idea that SCZMRDS and SCZNON-MRDS cohorts are biochemically distinct with regards to CHRM biology. Within the cortex, reductions in CHRM density were observed predominately within the SCZMRDS cohort for all binding densities measured. Within the striatum however, reductions of [3H] AF-DX 384 binding were observed in both SCZMRDS and SCZNONMRDS subjects compared to CTRLS. This suggests that in terms of CHRM density, the striatum is more complicated compared to the cortex. As CHRM1/4 agonist therapy represents a promising approach for treatment of both the positive and cognitive symptoms in people with schizophrenia, these results characterising discrete subgroups with regards to CHRM density…

Subjects/Keywords: schizophrenia; molecular psychiatry; cholinergic; energy metabolism; subgroups

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thomas, N. P. (2016). Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/58776

Chicago Manual of Style (16^th Edition):

Thomas, Natalie Paige. “Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism.” 2016. Doctoral Dissertation, University of Melbourne. Accessed January 19, 2021. http://hdl.handle.net/11343/58776.

MLA Handbook (7^th Edition):

Thomas, Natalie Paige. “Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism.” 2016. Web. 19 Jan 2021.

Vancouver:

Thomas NP. Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11343/58776.

Council of Science Editors:

Thomas NP. Investigating potential subgroups within the schizophrenias: a study of cholinergic muscarinic receptor density and energy metabolism. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/58776

University of Melbourne

8. KHIEW, HSU WEI. Cytokines, pain and regulation of inflammation.

Degree: 2015, University of Melbourne

URL: http://hdl.handle.net/11343/55543

► A mechanism termed the “inflammatory reflex” has been proposed as the basis for autonomic regulation of immune function. The efferent arm of this reflex –… (more)

▼ A mechanism termed the “inflammatory reflex” has been proposed as the basis for autonomic regulation of immune function. The efferent arm of this reflex – the neural-to-immune link – is thought to be the “cholinergic anti-inflammatory pathway”. According to this concept, an immune challenge is relayed by afferent nerves and/or by humoral signals to the brain, whereupon the brain drives efferent nerve fibres in the vagus that act ultimately to prevent the damaging consequences of excessive inflammation. The cholinergic anti-inflammatory pathway has been extensively studied in vivo and in vitro in terms of its protective effects against a wide range of inflammation-related diseases and immunomodulating functions. However, whether the in vivo anti-inflammatory effect of the cholinergic system can be mirrored and modelled by in vitro culture systems remains controversial and needs to be readdressed. The reported inhibitory effect of nicotine and the selective cholinergic agonist, AR-R17779, on the disease severity in murine collagen-induced arthritis could not be confirmed. Moreover, in a systematic in vitro study with LPS-stimulated murine macrophage populations and human monocyte/macrophage populations, it was found that nicotine did not reduce tumour necrosis factor (TNF) release even though in vivo it inhibited such expression in splenic macrophages. This suggests caution should be taken in the use of these particular in vitro systems as surrogate assays to study how the cholinergic pathway suppresses systemic inflammation. Inflammatory pain is multifaceted. Granulocyte macrophage-colony stimulating factor (GM-CSF) and TNF are potential drug targets for inflammatory and pain-related disorders based on the successful translation of animal data into clinical outcomes. In this thesis, TNF and GM-CSF were found to induce pain in an inflammatory footpad model in mice. It was found that neutrophil infiltration was required for TNF- and GM-CSF-induced pain. Colony stimulating factor-1 receptor signaling in macrophages was also required for inflammatory pain. In addition, TNF- and GM-CSF-induced pain was found to be abrogated in B6-KitW-sh/W-sh mice suggesting mast cell involvement. Ion channels, such as TRPV1, TRPA1, NaV1.7, and the neuropeptides, substance P and calcitonin gene-related peptide, were also found to be required for TNF- and GM-CSF-induced inflammatory pain development. The requirement for similar cell types and ion channels suggests that the pathways leading to TNF- and GM-CSF-driven pain might be similar and that TNF and GM-CSF may, in fact, be linked in this system. Utilizing GM-CSF-/- mice and an anti-GM-CSF mAb-based strategy, it was found that GM-CSF is required for TNF-induced pain. Moreover, treatment with anti-TNF mAb abolished GM-CSF-induced pain, indicating that TNF and GM-CSF are interdependent likely causing an amplification of the inflammatory response. Mice that lacked CCL17 were protected from inflammatory pain evoked by TNF and GM-CSF; CCL17 itself was able to induce inflammatory…

Subjects/Keywords: cytokines; pain; inflammation; cholinergic anti-inflammatory pathway

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

KHIEW, H. W. (2015). Cytokines, pain and regulation of inflammation. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/55543

Chicago Manual of Style (16^th Edition):

KHIEW, HSU WEI. “Cytokines, pain and regulation of inflammation.” 2015. Doctoral Dissertation, University of Melbourne. Accessed January 19, 2021. http://hdl.handle.net/11343/55543.

MLA Handbook (7^th Edition):

KHIEW, HSU WEI. “Cytokines, pain and regulation of inflammation.” 2015. Web. 19 Jan 2021.

Vancouver:

KHIEW HW. Cytokines, pain and regulation of inflammation. [Internet] [Doctoral dissertation]. University of Melbourne; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/11343/55543.

Council of Science Editors:

KHIEW HW. Cytokines, pain and regulation of inflammation. [Doctoral Dissertation]. University of Melbourne; 2015. Available from: http://hdl.handle.net/11343/55543

McGill University

9. Shore, Stephanie A. Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro.

Degree: PhD, Department of Physiology, 1984, McGill University

URL: https://escholarship.mcgill.ca/downloads/nc580q05r.pdf ; https://escholarship.mcgill.ca/concern/theses/70795b11n

►

L'influence modulatrice du système cholinergique local et des prostaglandines d'origine endogène sur l'activité contractile induite par l'histamine furent étudier in-vivo et in-vitro chez le muscle… (more)

Subjects/Keywords: Histamine; Cholinergic influences

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shore, S. A. (1984). Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro. (Doctoral Dissertation). McGill University. Retrieved from https://escholarship.mcgill.ca/downloads/nc580q05r.pdf ; https://escholarship.mcgill.ca/concern/theses/70795b11n

Chicago Manual of Style (16^th Edition):

Shore, Stephanie A. “Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro.” 1984. Doctoral Dissertation, McGill University. Accessed January 19, 2021. https://escholarship.mcgill.ca/downloads/nc580q05r.pdf ; https://escholarship.mcgill.ca/concern/theses/70795b11n.

MLA Handbook (7^th Edition):

Shore, Stephanie A. “Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro.” 1984. Web. 19 Jan 2021.

Vancouver:

Shore SA. Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro. [Internet] [Doctoral dissertation]. McGill University; 1984. [cited 2021 Jan 19]. Available from: https://escholarship.mcgill.ca/downloads/nc580q05r.pdf ; https://escholarship.mcgill.ca/concern/theses/70795b11n.

Council of Science Editors:

Shore SA. Factors Modulating Histamine Induced Contraction of Canine Airway Smooth Muscle In-Vivo and In-Vitro. [Doctoral Dissertation]. McGill University; 1984. Available from: https://escholarship.mcgill.ca/downloads/nc580q05r.pdf ; https://escholarship.mcgill.ca/concern/theses/70795b11n

University of British Columbia

10. Jourdain, Anne. Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat.

Degree: MS- MSc, Neuroscience, 1988, University of British Columbia

URL: http://hdl.handle.net/2429/27969

► Many basal forebrain and mesopontine tegmental cholinergic projection systems tend to overlap in their origins. This raises the possibility that these projection systems are collateralized… (more)

▼ Many basal forebrain and mesopontine tegmental cholinergic projection systems tend to overlap in their origins. This raises the possibility that these projection systems are collateralized to innervate divergent areas. In experiment one, the degree to which basal forebrain and mesopontine tegmental neurons that innervate the reticular thalamic nucleus have axons that collateralize to innervate the cortex as well was examined with a retrograde fluorescence labeling method combined with immunohistochemistry. A significant portion of the labeled neurons in the region of the nucleus basalis magnocellularis and pedunculopontine tegmental nucleus projecting to the reticular thalamic nucleus were observed to be also labeled (double-labeled) following intracortical tracer injections. Many of these double-labeled neurons displayed choline acetyltransferase choline acetyltransferase immunoreactivity. It was also shown that numerous basal forebrain neurons that innervated the reticular thalamic nucleus contained the calcium-binding protein, parvalbumin. These neurons tended to be located more rostrally than the ChAT immunoreactive neurons; primarily in the region of the ventral pallidum. There was some indication that parvalbumin-containing neurons in the basal forebrain that innervate the reticular thalamic nucleus also have axons that branch to innervate the cortex. Finally, none of the basal forebrain neurons innervating the reticular thalamic nucleus was found to contain somatostatin. In experiment two, the degree to which basal forebrain neurons have axons that collateralize to innervate the interpeduncular nucleus and hippocampus was examined with retrograde fluorescence labeling methods. Labeled neurons projecting to both of these limbic structures were observed only occasionally. Comparison of the distribution of single labeled neurons innervating each of these structures revealed that within the region of origin, in the horizontal limb of the diagonal band, neurons innervating the interpeduncular nucleus tended to be located dorsally to those innervating the hippocampus. The results of these experiments are discussed in relation to their anatomical and functional implications toward a greater understanding of the basal forebrain and mesopontine cholinergic and non-cholinergic projection systems.

Subjects/Keywords: Neurons; Cholinergic mechanisms; Cholinergic Fibers; Prosencephalon

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jourdain, A. (1988). Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/27969

Chicago Manual of Style (16^th Edition):

Jourdain, Anne. “Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat.” 1988. Masters Thesis, University of British Columbia. Accessed January 19, 2021. http://hdl.handle.net/2429/27969.

MLA Handbook (7^th Edition):

Jourdain, Anne. “Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat.” 1988. Web. 19 Jan 2021.

Vancouver:

Jourdain A. Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat. [Internet] [Masters thesis]. University of British Columbia; 1988. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2429/27969.

Council of Science Editors:

Jourdain A. Studies on the collateralization of some basal forebrain and mesopontine tegmental projection systems in the rat. [Masters Thesis]. University of British Columbia; 1988. Available from: http://hdl.handle.net/2429/27969

University of Otago

11. Kőszegi, Zsombor. Restorative effect of estrogen on basal forebrain cholinergic neurons .

Degree: 2011, University of Otago

URL: http://hdl.handle.net/10523/1887

► The basal forebrain cholinergic (BFC) system is one of the most important neurotransmitter systems in the brain. It has received much attention in the past… (more)

▼ The basal forebrain cholinergic (BFC) system is one of the most important neurotransmitter systems in the brain. It has received much attention in the past two decades, primarily for its role in learning, memory, attention and behavior. The BFC system has also been reported to be particularly vulnerable in neurodegenerative diseases, such as in Alzheimer’s disease (AD). The gonadal steroid, estrogen, is an essential contributor in controlling the vulnerability of the BFC system. Besides its classical or genomic mechanism, estrogen is known to have non-classical actions on intracellular signaling pathways. In this study, we investigated the ameliorative effects of estrogen treatment and the role of non-classical estrogen actions on BFC neurons in a neurodegenerative mouse model, in vivo. N-methyl-D-aspartate (NMDA) was injected unilaterally into the substantia innominata - nucleus basalis magnocellularis (SI-NBM) complex of the basal forebrain to elicit cholinergic cell death in the injected area and thus fiber loss in the ipsilateral cortex. An acute treatment of 17β-estradiol (E2) after the NMDA-induced lesion restored the ipsilateral cholinergic fiber density in the cortex in a time- and dose-dependent manner. Conversely, it did not have any effect on the cholinergic cell loss in the SI-NBM. The ameliorative action of E2 on cholinergic fiber loss was detected in both intact and gonadectomized young male and female mice, but not in aged animals. The E2-induced cholinergic fiber density restoration was also absent in neuron-specific estrogen receptor α (ERα) knockout mice. Selective blockade of the mitogen activated protein kinase (MAPK) and protein kinase A (PKA) pathways prevented E2’s ability to restore the cholinergic fiber density. Furthermore, activation of non-classical estrogen signaling by a non-classical pathway activator (estren) induced E2-like fiber restoration. Our findings demonstrate that estrogen restores the cholinergic fiber density in the cortex through a non-classical signaling mechanism after the loss of subcortical cholinergic input. Similar restorative effects were observed in young animals, irrespective of sex or endogenous estrogen levels. These observations reveal a critical role for non-classical estrogen signaling via ERα and MAPK-PKA pathways in BFC neurons, in vivo. Taken together, our study discloses important aspects relating to the vulnerability of the BFC system in neurodegenerative processes, such as AD or traumatic brain injury and might shed light on future medical treatments through the use of non-classical estrogen pathway activators. Advisors/Committee Members: Ábrahám, István (advisor).

Subjects/Keywords: estrogen; cholinergic; basal forebrain; neurodegeneration; non-classical; signaling pathway

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kőszegi, Z. (2011). Restorative effect of estrogen on basal forebrain cholinergic neurons . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1887

Chicago Manual of Style (16^th Edition):

Kőszegi, Zsombor. “Restorative effect of estrogen on basal forebrain cholinergic neurons .” 2011. Doctoral Dissertation, University of Otago. Accessed January 19, 2021. http://hdl.handle.net/10523/1887.

MLA Handbook (7^th Edition):

Kőszegi, Zsombor. “Restorative effect of estrogen on basal forebrain cholinergic neurons .” 2011. Web. 19 Jan 2021.

Vancouver:

Kőszegi Z. Restorative effect of estrogen on basal forebrain cholinergic neurons . [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10523/1887.

Council of Science Editors:

Kőszegi Z. Restorative effect of estrogen on basal forebrain cholinergic neurons . [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1887

12. Schöwe, Natália Mendes. Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação.

Degree: Mestrado, Farmacologia, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-11062014-145521/ ;

►

É conhecido que o sistema colinérgico, via receptor nicotínico a7, atua como atenuador do processo inflamatório. O lítio é utilizado no tratamento do transtorno afetivo… (more)

▼

É conhecido que o sistema colinérgico, via receptor nicotínico a7, atua como atenuador do processo inflamatório. O lítio é utilizado no tratamento do transtorno afetivo bipolar e, em microdoses, foi benéfico para o tratamento da doença de Alzheimer. O objetivo desse estudo foi avaliar os efeitos do tratamento crônico com microdoses de lítio para a formação de mediadores inflamatórios em cérebros de camundongos jovens, após injeção intraperitoneal de LPS, e se os receptores a7 estavam envolvidos nesse processo. Após sete meses de tratamento, foi observado que o grupo lítio-LPS apresentou formação da memória espacial, diferentemente do grupo água-LPS. Oito dias após a injeção de LPS, os níveis de IL-6, TNF-a, IL-10 e pGSK-3b/GSK-3b estavam iguais em todos os grupos, evidenciando que o processo inflamatório já estava terminado. Além disso, não houve diferença com relação à densidade de CAT e a7. Não foi possível comprovar ou excluir o efeito anti-inflamatório das microdoses de lítio. Portanto, o desenho experimental deverá ser ajustado.

It is known that the cholinergic system, via a7 nicotinic receptors, acts as attenuator of the inflammatory process. Lithium is used in the treatment of bipolar disorder and microdosing was beneficial for the treatment of Alzheimer\'s disease. The aim of this study was to evaluate the effects of chronic treatment with lithium microdoses for the formation of inflammatory mediators in the brain of young mice after intraperitoneal injection of LPS, and if a7 receptors were involved in this process. After seven months of treatment, animals treated with lithium-LPS presented spatial memory, while water-LPS animals did not. Eight days after the induction of acute inflammation levels of IL-6, TNF-a, IL-10 and pGSK-3b/GSK-3b were the same in all groups, indicating that the inflammatory process had been completed. Still, there was no difference with respect to the density of CAT and a7. It was not possible to confirm or exclude the anti-inflammatory effect of microdoses of lithium. So, the experimental design will be adjusted.

Advisors/Committee Members: Viel, Tânia Araújo.

Subjects/Keywords: Camundongos; Cholinergic receptors; Inflamação; Inflammation; Lithium; Lítio; Mice; Receptores colinérgicos

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Schöwe, N. M. (2013). Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42136/tde-11062014-145521/ ;

Chicago Manual of Style (16^th Edition):

Schöwe, Natália Mendes. “Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação.” 2013. Masters Thesis, University of São Paulo. Accessed January 19, 2021. http://www.teses.usp.br/teses/disponiveis/42/42136/tde-11062014-145521/ ;.

MLA Handbook (7^th Edition):

Schöwe, Natália Mendes. “Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação.” 2013. Web. 19 Jan 2021.

Vancouver:

Schöwe NM. Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação. [Internet] [Masters thesis]. University of São Paulo; 2013. [cited 2021 Jan 19]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-11062014-145521/ ;.

Council of Science Editors:

Schöwe NM. Relação entre o tratamento crônico com lítio e papel do sistema colinérgico na neuroinflamação. [Masters Thesis]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42136/tde-11062014-145521/ ;

13. Kim, Kamin. Cholinergic Modulation of Attention.

Degree: PhD, Psychology, 2015, University of Michigan

URL: http://hdl.handle.net/2027.42/111387

► Rodent studies indicate that cholinergic inputs to frontoparietal cortex play an important role in signal detection, especially in challenging conditions. fMRI studies have likewise shown… (more)

▼ Rodent studies indicate that cholinergic inputs to frontoparietal cortex play an important role in signal detection, especially in challenging conditions. fMRI studies have likewise shown frontoparietal activity in humans under task conditions parallel to those used in the rodent studies. While these parallels are suggestive, the degree to which the fMRI activation patterns seen in humans reflect cholinergic activity remains unknown. The studies in this dissertation provide stronger evidence for cholinergic influences on the brain systems supporting attention in humans, and begin to delineate how those influences may differ by brain region and interact with other (e.g. dopaminergic) influences to shape cognition and behavior. First, an electroencephalography study showed that gamma synchronization, which previous studies have linked to cholinergic activity and attentional control, increases in response to a distractor challenge. Furthermore, across participants, greater increases in gamma synchronization in parietal cortex were associated with better distractor resistance, whereas greater increases in gamma dispersion in right prefrontal cortex were associated with greater response time variations thought to reflect difficulty in maintaining consistent control. Another series of experiments leveraged variability in cholinergic integrity (measured using PET) in Parkinson’s patients as a natural experiment to determine cholinergic contributions to different aspects of attention and cognitive control. Thalamic cholinergic integrity made the strongest independent contribution to variation in the ability to detect signals under perceptual challenge, whereas cortical cholinergic integrity was the best independent predictor of the ability to resist content-rich distractors likely to draw attention away from the target signal. Exploratory analyses suggested that parietal cholinergic integrity might play an especially important role in resisting these distractors, consistent with the electroencephalography study results. Finally, a secondary data analysis of a larger sample suggested that in conditions making strong demands on executive control, there may be mutual compensation between cholinergic and dopaminergic systems. To summarize, the present findings provide further evidence for cholinergic contributions to frontoparietal brain systems supporting signal detection, attention, and cognitive control, more precisely define the contributions of thalamic, prefrontal, and parietal inputs, and suggest the possibility of mutual compensation with the dopaminergic system in situations of high executive demand. Advisors/Committee Members: Gehring, William J. (committee member), Lustig, Cindy Ann (committee member), Bohnen, Nicolaas I. (committee member), Sarter, Martin Friedrich (committee member).

Subjects/Keywords: cholinergic; gamma oscillations; attentional control; Psychology; Social Sciences

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, K. (2015). Cholinergic Modulation of Attention. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/111387

Chicago Manual of Style (16^th Edition):

Kim, Kamin. “Cholinergic Modulation of Attention.” 2015. Doctoral Dissertation, University of Michigan. Accessed January 19, 2021. http://hdl.handle.net/2027.42/111387.

MLA Handbook (7^th Edition):

Kim, Kamin. “Cholinergic Modulation of Attention.” 2015. Web. 19 Jan 2021.

Vancouver:

Kim K. Cholinergic Modulation of Attention. [Internet] [Doctoral dissertation]. University of Michigan; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2027.42/111387.

Council of Science Editors:

Kim K. Cholinergic Modulation of Attention. [Doctoral Dissertation]. University of Michigan; 2015. Available from: http://hdl.handle.net/2027.42/111387

University of Georgia

14. Kellock, Kristen. Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/23886

► Botulinum neurotoxin (BoNT), a potent neurotoxin produced under anaerobic conditions by the bacteria Clostridium botulinum, is the most lethal toxin known to man. The primary… (more)

▼ Botulinum neurotoxin (BoNT), a potent neurotoxin produced under anaerobic conditions by the bacteria Clostridium botulinum, is the most lethal toxin known to man. The primary target site for BoNT action is the cholinergic nerve terminal that innervates the neuromuscular junction. Botulinum neurotoxin inhibits the formation of the SNARE complex which is critical to the release of vesicular acetylcholine (ACh). Without the release of ACh, flaccid paralysis (botulism) ensues. Because of the potential usefulness of toxin in clinical conditions involving cholinergic terminals in the autonomic nervous system, we are testing the suitability of embryonic chick sympathetic ganglia for BoNT study. To achieve this, it was first necessary to develop a protocol for processing ganglia. We tested two different ganglia preparations. The first, a synaptosome-enriched fraction and the second, sympathetic explants plated in poly-lysine coated plates. These protocols were then tested to characterize the neurochemistry, as well as the capacity for vesicular release in sympathetic ganglia isolated from 9-10 day old chick embryos. Using protein immunochemistry we have identified SNAP-25, VAMP and syntaxin - all components of the SNARE complex; synaptotagmin - the putative calcium sensor in both the synaptosome-enriched fraction and in the explant preparations. Choline acetyltransferase - an enzyme involved in the synthesis of ACh in this embryonic preparation and the vesicular acetylcholine transporter protein were only identified in the explant preparation. To determine the sensitivity of the sympathetic ganglia preparations to BoNT/A and BoNT/C substrate cleavage assays were utilized. The synaptosome-enriched fraction did not result in BoNT/A induced cleavage of SNAP-25. The isolated explants were grown for a minimum of 4 days, and then exposed to toxin for 2 hours. The toxin was then removed and media was returned for variable incubation periods. Based on our findings we can conclude that the day 10 embryonic chick sympathetic ganglia explants are susceptible to BoNT/A and C binding, internalization and protein targeting.

Subjects/Keywords: Botulinum neurotoxin; SNARE; cholinergic; embryonic chick sympathetic ganglia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kellock, K. (2014). Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/23886

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kellock, Kristen. “Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study.” 2014. Thesis, University of Georgia. Accessed January 19, 2021. http://hdl.handle.net/10724/23886.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kellock, Kristen. “Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study.” 2014. Web. 19 Jan 2021.

Vancouver:

Kellock K. Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10724/23886.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kellock K. Assessing the suitability of embryonic chick sympathetic ganglia for botulinum toxin study. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/23886

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

15. Liu, Yishi. A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans.

Degree: PhD, Biology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9187

► The nervous system coordinates a sequence of muscle movements to give rise to animal behaviors. In complex invertebrates or lab-studied vertebrates, due to the large… (more)

▼ The nervous system coordinates a sequence of muscle movements to give rise to animal behaviors. In complex invertebrates or lab-studied vertebrates, due to the large number of cells in their nervous systems and the complexities of their behaviors, it is difficult to address how circuits process information to direct each motor output of the behavior. In this dissertation, I used the Caenorhabditis elegans male copulation behavior as a model to address how a compact circuit coordinates different behavioral programs. Insertion of a male copulatory organ into a suitable mate is a conserved and necessary behavioral step for most terrestrial mating. However, the detailed molecular and cellular mechanisms for this distinct social interaction have not been elucidated in any animal. During mating, the C. elegans male cloaca is positioned over the hermaphrodite’s vulva as he attempts to insert his copulatory spicules repetitively. Rhythmic spicule thrusts cease when insertion is sensed. Circuit components consisting of sensory/motor neurons and sex muscles for these steps have been previously identified, but it was unclear how their outputs are integrated to generate a coordinated behavior pattern. Here, I show that contraction of the male oblique muscles is required to sustain genital contact between the sexes. These muscles are innervated by the postcloacal sensilla (p.c.s.) sensory/motor neurons, which secret ACh to activate the levamisole-sensitive AChR and the ACR-16-containing ionotropic AChR on the oblique muscles. For spicules to rhythmically thrust during genital contact, activity of the oblique muscles and the gubernacular muscles is transmitted to the spicule protractor muscles instantaneously via gap junctions between these muscles and causes shallow protractor contractions. The rhythmic protractor contractions eventually switch to sustained contraction, as the SPC sensory-motor neurons integrate information of spicule position at the vulva with inputs from the hook and cloacal sensilla. The ERG-like K+ channel, UNC-103, which decreases the spicule circuit excitability, is likely to set a threshold requirement for integration of these inputs, so that sustained spicule muscle contraction is not stimulated by fewer inputs. In addition, I demonstrate that a cholinergic signaling pathway mediated by a muscarinic acetylcholine receptor, GAR-3, is used to enhance the ionotropic AChRs-mediated fast synaptic transmission in the copulation circuit. GAR-3 is expressed in multiple cells of the copulation circuit, but mainly in the cholinergic p.c.s. neurons and SPC neurons. Activation of GAR-3 is coupled to Gαq to trigger downstream signal transduction events that modulate neurotransmitter release from these neurons. Males with a loss-of-function allele of the gar-3 gene are defective in inserting their spicules into the hermaphrodite’s vulva efficiently. Since the p.c.s. neurons regulate the male’s contact with the hermaphrodite’s vulva, and the SPC neurons are required for spicule insertion during mating, GAR-3 probably… Advisors/Committee Members: Garcia, Luis Rene (advisor), Grau, James (committee member), Perkins, Brian (committee member), Smotherman, Michael (committee member).

Subjects/Keywords: C. elegans; behavior; male mating; cholinergic; gap junction

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liu, Y. (2012). A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9187

Chicago Manual of Style (16^th Edition):

Liu, Yishi. “A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans.” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9187.

MLA Handbook (7^th Edition):

Liu, Yishi. “A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans.” 2012. Web. 19 Jan 2021.

Vancouver:

Liu Y. A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9187.

Council of Science Editors:

Liu Y. A Cholinergic Sensory-Motor Circuit Controls the Male Copulation Behavior in C. elegans. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9187

Texas A&M University

16. Midkiff, James. Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans.

Degree: MS, Biology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/148382

► All complex organisms possess a nervous system which they use to monitor environmental and internal stimuli. In higher vertebrates, the nervous system is comprised of… (more)

▼ All complex organisms possess a nervous system which they use to monitor environmental and internal stimuli. In higher vertebrates, the nervous system is comprised of billions of cells which form highly plastic neural networks from their synapses. These large neural circuits modulate complex behaviors. The nematode roundworm Caenorhabditis elegans uses a small but highly-interconnected nervous system to carry out complex behaviors. The nervous system of C. elegans is a tractable model to determine the effects of changes on a nervous system at the systemic, cellular, genetic, and molecular levels. The C. elegans male’s nervous system detects environmental conditions, mating cues, attractants, repellents, and the location and composition of possible food sources and integrates these inputs to compute the decision of whether or not to mate. Mating behavior in the C. elegans male is regulated at a number of steps by cholinergic signaling from various sensory and sensory-motor neurons, but a comprehensive model of how cholinergic signaling controls this circuit has not yet been elucidated. Previous studies have thoroughly dissected the cellular structure, neural connectivity, and signaling pathways of the male’s peripheral circuits located in the genital regions of the animal’s tail. However, no studies have been conducted to determine what role the cephalic cholinergic neurons have in regulating mating behavior. I hypothesized that cephalic cholinergic neurons exert regulatory control over the male-specific mating circuit. I inserted the transmembrane light-activated ion pore Channelrhodopsin-2 fused to YFP and expressed from the Punc-17small promoter into these neurons and selectively stimulated them using high-intensity blue light. Stimulation induced mating-like behaviors in the male tail consistent with behaviors seen during copulation with a hermaphrodite. Using behavioral assays, I demonstrated that these behaviors were male-specific and only occurred after direct stimulation in the absence of a hermaphrodite. Incidence of mating-like behaviors increased significantly as the worm aged, and the mating circuit retained a memory of the stimulus, indicated by the latency between stimulation and onset of mating-like behaviors. Brief food deprivation, which normally downregulates excitability of the mating circuit via UNC-103 ERG-like K+ channels, caused an unexpected increase in the number of blue light-stimulated behaviors displayed. Pharmacological assays using acetylcholine (ACh) agonists showed that stimulation of the cephalic cholinergic neurons increased propensity for spicule protraction in the presence of an ACh agonist, and partially restored the decline in spicule protraction associated with temporary food deprivation. I sought to identify the cephalic cholinergic neuron or neurons responsible for regulating mating-like behavior in the tail circuits. I looked for a reduction in mating-like behaviors after stimulation after removal of a cephalic cholinergic neuron pair via laser micro-ablation. Two… Advisors/Committee Members: Garcia, Luis R (advisor), Lekven, Arne C (committee member), Park, William D (committee member).

Subjects/Keywords: cholinergic; cephalic; behavior; motivation; mating; male; C. elegans

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Midkiff, J. (2012). Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/148382

Chicago Manual of Style (16^th Edition):

Midkiff, James. “Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans.” 2012. Masters Thesis, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/148382.

MLA Handbook (7^th Edition):

Midkiff, James. “Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans.” 2012. Web. 19 Jan 2021.

Vancouver:

Midkiff J. Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/148382.

Council of Science Editors:

Midkiff J. Artificial Stimulation of Cephalic Cholinergic Sensory Neurons Induces Mating-Like Motor Responses in Male Caenorhabditis elegans. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/148382

University of Texas Southwestern Medical Center

17. Pollok, Robert Harding. Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/2727

► During starvation, C. elegans adjust their behavior in order to survive. Using the starvation-sensitive gpb-2(ad541) loss-of-function mutant, components in a starvation-signaling pathway were identified. The… (more)

Subjects/Keywords: Caenorhabditis elegans Proteins; Cholinergic Agents; Signal Transduction; Starvation

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pollok, R. H. (2013). Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/2727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pollok, Robert Harding. “Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 19, 2021. http://hdl.handle.net/2152.5/2727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pollok, Robert Harding. “Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2.” 2013. Web. 19 Jan 2021.

Vancouver:

Pollok RH. Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152.5/2727.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pollok RH. Starvation-Signaling in the Nematode Caenorhabditis Elegans Using Regulator of G-Proteins GPB-2. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/2727

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. LaSarge, Candi Lynn. GABAergic systems in a model of age-related cognitive impairment.

Degree: PhD, Psychology, 2012, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9217

► With medical advancements extending the life span, age-related cognitive decline is a growing problem for the United States. A rat model of cognitive aging was… (more)

▼ With medical advancements extending the life span, age-related cognitive decline is a growing problem for the United States. A rat model of cognitive aging was used to investigate the GABAergic neurotransmitter system in relation to changes in learning and memory functions. Confocal stereology was used to determine the number of GABAergic and cholinergic projection neurons in the rostral basal forebrain of spatially characterized young and aged male F344 rats. The GABAergic system was then assessed as a potential target for improving age-related cognitive decline using an odor discrimination task sensitive to decline in aging. Performance of aged rats was impaired compared to young rats on the spatial version of the Morris water maze. Notably, a high degree of variability in individual abilities was observed among aged rats such that some aged rats performed on par with young (aged-unimpaired) and others performed outside the range of young, demonstrating impairment (aged-impaired). The number of basal forebrain neurons expressing multiple immunomarkers for GABAergic septohippocampal projection cells was selectively increased in aged-impaired rats in comparison to both young and aged-unimpaired rats. Indeed, among aged rats, worse performance in the water maze was reliably associated with higher GABAergic cell number. The number of cholinergic neurons, quantified in adjacent sections did not differ as a function of chronological age or cognitive status. These data suggest that aging can dysregulate GABAergic systems in circuitry important for learning and memory and such alterations may contribute to age-related cognitive decline. To test whether the GABAergic system may be a viable target for treating age-related cognitive decline, a second cohort of young and aged rats was characterized in an odor discrimination task. Similar to aged rat water maze performance, some aged rats performed odor learning discrimination problems on par with the young cohort (i.e. aged-unimpaired) and some aged rats were impaired compared to young (i.e. aged-impaired). Using a within-subjects design, the GABA(B) antagonist, CGP 55845 completely ameliorated odor discrimination learning deficits in aged-impaired rats in a dose-dependent manner. These data support the hypothesis that the GABAergic system should be a novel target for therapies aimed at treating age-related cognitive decline. Advisors/Committee Members: Bizon, Jennifer L. (advisor), Setlow, Barry (committee member), Griffith, William H. (committee member), Packard, Mark G. (committee member).

Subjects/Keywords: Aging; GABA; cholinergic; cognition

…Cholinergic and GABAergic systems and olfactory learning and memory… …II 2 3 4 5 8 8 11 INTEGRITY OF CHOLINERGIC AND GABAERGIC BASAL FORBRAIN NEURONS IN AGING… …Interactions of basal forebrain cholinergic-GABAergic systems: Implications for cognition… …Role of cholinergic system in age-related cognitive impairment… …116 xii LIST OF FIGURES FIGURE 1 2 3 4 5 6 7 8 Page Cholinergic and GABAergic…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

LaSarge, C. L. (2012). GABAergic systems in a model of age-related cognitive impairment. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9217

Chicago Manual of Style (16^th Edition):

LaSarge, Candi Lynn. “GABAergic systems in a model of age-related cognitive impairment.” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9217.

MLA Handbook (7^th Edition):

LaSarge, Candi Lynn. “GABAergic systems in a model of age-related cognitive impairment.” 2012. Web. 19 Jan 2021.

Vancouver:

LaSarge CL. GABAergic systems in a model of age-related cognitive impairment. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9217.

Council of Science Editors:

LaSarge CL. GABAergic systems in a model of age-related cognitive impairment. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9217

University of Toronto

19. Takkala, Petri. Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity.

Degree: 2012, University of Toronto

URL: http://hdl.handle.net/1807/33553

►

Activation of muscarinic acetylcholine receptors (mAChRs) has pronounced effects on GABAergic interneurons, including depolarization of their resting membrane potential, and increasing their action potential and… (more)

Subjects/Keywords: dmLTP; cholinergic; mAChR; neuromodulation; plasticity; GABA; 0317; 0379

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Takkala, P. (2012). Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/33553

Chicago Manual of Style (16^th Edition):

Takkala, Petri. “Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity.” 2012. Masters Thesis, University of Toronto. Accessed January 19, 2021. http://hdl.handle.net/1807/33553.

MLA Handbook (7^th Edition):

Takkala, Petri. “Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity.” 2012. Web. 19 Jan 2021.

Vancouver:

Takkala P. Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity. [Internet] [Masters thesis]. University of Toronto; 2012. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1807/33553.

Council of Science Editors:

Takkala P. Cholinergic Neuromodulation of Activity-dependent Disinhibition-mediated Plasticity. [Masters Thesis]. University of Toronto; 2012. Available from: http://hdl.handle.net/1807/33553

University of Toronto

20. Rapps, Joshua A. Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis.

Degree: 2018, University of Toronto

URL: http://hdl.handle.net/1807/91465

►

Insulin resistance and insulin deficiency are key determinants of type 2 diabetes. Insulin resistance is manifested by attenuation of insulin signaling. A major insulin signaling… (more)

Subjects/Keywords: ChAT; Cholinergic; Dorsal Vagal Complex; Insulin; PI3K; PTEN; 0409

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rapps, J. A. (2018). Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/91465

Chicago Manual of Style (16^th Edition):

Rapps, Joshua A. “Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis.” 2018. Masters Thesis, University of Toronto. Accessed January 19, 2021. http://hdl.handle.net/1807/91465.

MLA Handbook (7^th Edition):

Rapps, Joshua A. “Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis.” 2018. Web. 19 Jan 2021.

Vancouver:

Rapps JA. Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis. [Internet] [Masters thesis]. University of Toronto; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1807/91465.

Council of Science Editors:

Rapps JA. Assessing the role of PTEN in cholinergic neurons in glucose and energy homeostasis. [Masters Thesis]. University of Toronto; 2018. Available from: http://hdl.handle.net/1807/91465

21. V. Alari. CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.

Degree: 2013, Università degli Studi di Milano

URL: http://hdl.handle.net/2434/215883

► The α7 nicotinic acetylcholine receptor (α7 nAChR) has a key role in the innate immune system’s inflammatory response, as part of “cholinergic anti-inflammatory pathway”: a… (more)

▼ The α7 nicotinic acetylcholine receptor (α7 nAChR) has a key role in the innate immune system’s inflammatory response, as part of “cholinergic anti-inflammatory pathway”: a process by which acetylcholine from the vagus nerve reduces the release of the pro-inflammatory cytokine TNFα, thus allowing for a controlled response to infection. The CHRNA7 gene, in humans, is partially duplicated from exon 5-10 and forms an hybrid with four exons (D-A) of a novel gene, FAM7A. This new gene, CHRFAM7A, which is located in the opposite orientation, at 1.6 Mb, from CHRNA7, is not present on every chromosome 15 and a polymorphic variant, in linkage disequilibrium with a 2bp deletion in exon 6, in the same orientation to the CHRNA7 gene, has been described in a cohort of patients with bipolar disorders and schizophrenia. THP-1 monocytic-like cell line expresses only CHRFAM7A, which was down-regulated on treatment with LPS, by a direct transcriptional mechanism reliant on NF-kB. This effect has been confirmed in primary monocytes and macrophages cell cultures, where CHRFAM7A is expressed 200-1000 times more than CHRNA7. Here, the conventional α7 subunit was up-regulated by LPS treatment, thus suggesting the involvement of CHRFAM7A in the regulation of cell surface α7 receptors’ level (a mechanism unique to humans) and the ability of immune cells to respond to acetylcholine, released from the vagus nerve, during infection. This hypothesis seems to be supported by recent works showing that the duplicated form may have a dominant negative effect on the activity of α7 nAChR. Infact, co-expression of CHRFAM7A with α7 results in a significant reduction of the Ach-evoked currents, suggesting the presence of heteromeric non functional receptors at the plasma membrane. The promoter region that regulates the expression of CHRFAM7A is still unknown. To try to identify and characterize this region, 5'-RACE experiments were carried out to map the CHRFAM7A mRNA 5’UTR. RNA was extracted from three different cell lines: THP-1 cells, primary human macrophages and SHSY5Y neuroblastoma cell line. Multiple transcription start sites were identified, depending on the cell line used, suggesting the existence of alternative promoters. A series of constructs that recapitulate the mapping of the CHRFAM7A regulatory region, according to the transcription start sites identified, was also generated. They were cloned into a reporter vector and their functionality was tested by transient transfection both in THP-1 and SHSY5Y cell models. Through these experiments, an intronic region (-702/-208 bp from ATG codon, in exon B) and an Alu sequence (-1155/-821 bp) were identified as negative regulators of reporter gene transcription. Future experiments will allow us to identify other regulatory sites, important for proper CHRFAM7A gene expression in different tissues. Furthermore, two variants exist for CHRFAM7A gene, due to alternative splicing, that gives rise to two protein products of predicted 36 and 47 KDa, whose function is currently unknown. The N-terminally… Advisors/Committee Members: tutor:D. Fornasari, coordinatore: A. Gianni, FORNASARI, DIEGO MARIA MICHELE, GIANNI, ALESSANDRO.

Subjects/Keywords: CHRFAM7A; cholinergic anti-inflammatory pathway; CHRNA7; alpha7; Settore BIO/14 - Farmacologia

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alari, V. (2013). CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/215883

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alari, V.. “CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.” 2013. Thesis, Università degli Studi di Milano. Accessed January 19, 2021. http://hdl.handle.net/2434/215883.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alari, V.. “CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.” 2013. Web. 19 Jan 2021.

Vancouver:

Alari V. CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2434/215883.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alari V. CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/215883

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago

22. Kim, SooHyun. Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies .

Degree: 2013, University of Otago

URL: http://hdl.handle.net/10523/4180

► Basal forebrain cholinergic (BFC) neurons play a critical role in learning and memory and are highly affected in the age-associated neurodegenerative disorders such as Alzheimer’s… (more)

▼ Basal forebrain cholinergic (BFC) neurons play a critical role in learning and memory and are highly affected in the age-associated neurodegenerative disorders such as Alzheimer’s disease (AD). Previous neurodegenerative lesion studies have demonstrated a neuroprotective effect of gonadal steroid,17β-estradiol (E2), treatment on cholinergic neurons. However, these lesion models lacked selective damage to cholinergic neurons, limiting the interpretation of behavioural deficits observed after such a lesion and the correlation with the neuroprotective potential of E2. In this study, we have examined the effect of E2 treatment on cholinergic neurons and associated learning behaviours using an in vivo mouse model with a selective cholinergic lesion in the nucleus basalis magnocellularis (NBM). Such a model was achieved by applying a novel, highly selective mouse cholinotoxin, mu p75-Saporin (mp75SAP), by means of a microinjection into the NBM of ovariectomised adult female mice. Unilateral injection of mp75-SAP resulted in cholinergic cell loss in the NBM and ipsilateral cholinergic fibre loss in the somatosensory cortex. The 0.9μg/μl mp75-SAP exhibited ~70% of cholinergic cell loss in the NBM and ~75% of cholinergic fibre loss in the cortex. A single injection of E2 1h after mp75SAP-induced lesion increased the ipsilateral cholinergic fibre density in the somatosensory cortex by approximately 10% but did not have an effect on cholinergic cell loss in the NBM. The functional consequences of an E2 treatment on selective cholinergic lesion in the NBM was tested by performing a single-pellet skilled-reaching task and a novel object recognition test which tested the animals’ learning behaviours. Mice given a bilateral injection of mp75SAP into the NBM demonstrated profound learning deficits in both tests. The acute E2 treatment did not have an effect on the mp75SAP lesion-induced learning deficits. However, the cholinergic fibres in the somatosensory cortex were restored by the E2 treatment following bilateral mp75SAP-induced cholinergic lesion in the NBM. These findings demonstrate that a single E2 treatment is able to restore the basal forebrain fibre loss in the somatosensory cortex following the cholinergic lesion in the NBM, but does not have any functional effect regarding the motor skill and discrimination learning behaviours. Overall, this study aids in understanding of E2-induced neuroprotective actions that may have therapeutic relevance in AD-associated BFC degeneration and progressive cognitive decline. Advisors/Committee Members: Ábrahám, István (advisor).

Subjects/Keywords: cholinergic; estradiol; nucleus; basalis; magnocellularis; basalforebrain; lesions; neuroprotective

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, S. (2013). Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies . (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/4180

Chicago Manual of Style (16^th Edition):

Kim, SooHyun. “Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies .” 2013. Masters Thesis, University of Otago. Accessed January 19, 2021. http://hdl.handle.net/10523/4180.

MLA Handbook (7^th Edition):

Kim, SooHyun. “Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies .” 2013. Web. 19 Jan 2021.

Vancouver:

Kim S. Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies . [Internet] [Masters thesis]. University of Otago; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10523/4180.

Council of Science Editors:

Kim S. Neuroprotective effect of estradiol in mice with selective cholinergic lesions in nucleus basalis magnocellularis: morphological and behavioural studies . [Masters Thesis]. University of Otago; 2013. Available from: http://hdl.handle.net/10523/4180

University of California – San Diego

23. Wright, Morgan. Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory.

Degree: Biology, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/65x6b030

► The medial septum (MS) projects to the hippocampus (HC) and is involved in generating hippocampal theta. Two distinct populations of neurons in the MS have… (more)

Subjects/Keywords: Biology; Cholinergic; Hippocampus; Medial septum; Memory; Optogenetics; Theta

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wright, M. (2018). Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/65x6b030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wright, Morgan. “Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory.” 2018. Thesis, University of California – San Diego. Accessed January 19, 2021. http://www.escholarship.org/uc/item/65x6b030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wright, Morgan. “Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory.” 2018. Web. 19 Jan 2021.

Vancouver:

Wright M. Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Jan 19]. Available from: http://www.escholarship.org/uc/item/65x6b030.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wright M. Exploring the Role of Septal Parvalbumin and Cholinergic Neurons in Hippocampal Theta Oscillations and Hippocampal-dependent Memory. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/65x6b030

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

24. Stiver, Mikaela. An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems.

Degree: MS, Department of Psychology, 2016, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9823

► Consolidated memories may become destabilized during reactivation, resulting in a transient state of instability, presumably for updating or maintenance. Resilient engrams, such as remote memories,… (more)

Subjects/Keywords: object memory; destabilization; cholinergic; ubiquitin proteasome system; spontaneous object recognition

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stiver, M. (2016). An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9823

Chicago Manual of Style (16^th Edition):

Stiver, Mikaela. “An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems.” 2016. Masters Thesis, University of Guelph. Accessed January 19, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9823.

MLA Handbook (7^th Edition):

Stiver, Mikaela. “An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems.” 2016. Web. 19 Jan 2021.

Vancouver:

Stiver M. An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems. [Internet] [Masters thesis]. University of Guelph; 2016. [cited 2021 Jan 19]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9823.

Council of Science Editors:

Stiver M. An Exploration of the Mechanisms of Object Memory Destabilization: Involvement of the Cholinergic and Ubiquitin Proteasome Systems. [Masters Thesis]. University of Guelph; 2016. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/9823

Univerzitet u Beogradu

25. Ivanović, Saša, 1969-. Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika.

Degree: Fakultet veterinarske medicine, 2015, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:7283/bdef:Content/get

►

Veterinarska medicina - Farmakologija i toksikologija / Veterinary medicine - Pharmacology and Toxicology

Gabaergički i holinergički sistem u neuro-mišićnom sistemu parazitskih nematoda su glavna ciljna… (more)

▼

Veterinarska medicina - Farmakologija i toksikologija / Veterinary medicine - Pharmacology and Toxicology

Gabaergički i holinergički sistem u neuro-mišićnom sistemu parazitskih nematoda su glavna ciljna mesta delovanja antihelmintika. U okviru holinergičkog sistema nematoda, farmakološki značaj ima pre svega nikotinski-acetilholinski receptor (nAChR). S druge strane, GABA-receptor parazitskih nematoda je prvobitno označen kao glavno mesto dejstva avermektina i milbemicina. Međutim, vremenom se pokazalo da ovi lekovi deluju i na jedan potpuno nov, do tada neopisani glutamat-zavisni hloridni jonski kanal u farinksu nematoda. Pored toga, značajno je da neki aktivni principi etarskih ulja imaju dokazana antiparazitska svojstva, i da postoje indicije da deluju upravo preko ova dva receptorska sistema. Osnovni problemi koji danas ugrožavaju antiparazitsku terapiju su razvoj rezistencije i često ispoljavanje toksičnih efekata antiparazitskih lekova. Da bi se bolje razumeli mehanizmi dejstva gabaergičkih i holinergičkih antihelmintika ispitali smo farmakološke karakteristike predstavnika ove dve grupe lekova na neuro-mišićnom preparatu velike nematode svinja Ascaris suum. Takođe, značajno je bilo komparativno ispitati razlike u dejstvu gabaergičkih i holinergičkih antihelmintika na odgovarajuće receptore sisara (ispitivanja su izvršena na izolovanoj dijafragmi i ileumu pacova) i na taj način analizirati mehanizme njihovih neželjenih efekata. U našim istražvanjima mereni su efekti kontrakcije ili relaksacije izolovanih preparata i odgovarajućim statističkim metodama obrađivani dobijeni rezultati (nelinearna regresija, ANOVA, t-tets). Na osnovu rezultata dobijenih u ispitivanjima doneti su sledeći zaključci: (1) Agonisti L, N i B tipa nikotinskog-acetilholinskog receptora (nAChR) nematoda, ispitivani na modelu neuro-mišićnog preparata A. suum, ispoljili su različitu efikasnost. Najvišu efikasnost u prvoj grupi ispitivanih agonista, ispoljio je pirantel (agonista L-tipa nikotinskog receptora EC50=0.010μM, Emax=2.5g), zatim befinijum (agonista B-tipa nikotinskog receptora EC50=0.37μM, Emax=2.7g) i na kraju acetilholin (endogeni neurotransmiter), agonista sva tri tipa L, N i B nikotinskog receptora (EC50=6.12–6.45μM, Emax=1.71–2.07g). Najvišu efikasnost u drugoj grupi ispitivanih agonista nikotinskog receptora ispoljio je tribendimidin (najverovatnije agonista L-tipa nikotinskog receptora, EC50=0.064μM, Emax=1.29g), zatim levamizol (agonista L-tipa nikotinskog receptora, EC50=0.34μM, Emax=0.68g) i na kraju nikotin (agonista N-tipa nikotinskog receptora, EC50=4.99μM, Emax=1.07g). (2) Nikotinski-acetilholinski receptor A. suum, na koji deluju tribendimidin i nikotin ispoljava osobine oba tipa nAChR sisara: a) karakteristike mišićnog tipa nAChR sisara, jer je osetljiv pre svega na pankuronijum ali i na tubokurarin; b) karakteristike neuronskog tipa nAChR sisara, jer je osetljiv pre svega na mekamilamin ali i na heksametonijum. (3) GABA izaziva dozno-zavisnu relaksaciju neuro-mišićnog preparata A.suum, sa vrednošću srednje EC50 od…

Advisors/Committee Members: Trailović, Saša, 1963-.

Subjects/Keywords: antiparasitics; mechanism of action; toxicity; GABA agonists; cholinergic agonists; carvacrol; tribendimidine

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ivanović, Saša, 1. (2015). Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:7283/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ivanović, Saša, 1969-. “Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika.” 2015. Thesis, Univerzitet u Beogradu. Accessed January 19, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:7283/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ivanović, Saša, 1969-. “Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika.” 2015. Web. 19 Jan 2021.

Vancouver:

Ivanović, Saša 1. Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika. [Internet] [Thesis]. Univerzitet u Beogradu; 2015. [cited 2021 Jan 19]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:7283/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ivanović, Saša 1. Komparativno ispitivanje mehanizama antiparazitskog i toksičnog dejstva gabaergičkih i holinergičkih antihelmintika. [Thesis]. Univerzitet u Beogradu; 2015. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:7283/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Fisher, Beth Mary. Role of prefrontal cortex and cholinergic modulation in attentional performance in rats.

Degree: PhD, 2018, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/274607

► The present thesis investigates the role of the prefrontal cortex and cholinergic modulation in attentional performance, and to a lesser extent, inhibitory response control, in… (more)

▼ The present thesis investigates the role of the prefrontal cortex and cholinergic modulation in attentional performance, and to a lesser extent, inhibitory response control, in rats. A greater understanding of these functions is important for the effective treatment of attentional and impulsive control deficits, present in a range of neuropsychiatric disorders. For this field to progress, the assessment of attentional performance in a similar manner across humans and animals is crucial. In the present thesis, attentional performance was assessed on the novel, touchscreen-based rodent continuous performance task (rCPT), which assesses sustained, focused attention in essentially an identical manner to CPTs commonly used in the clinic. Findings were compared to performance on the well-characterised 5-choice serial reaction time task (5-CSRTT), which assesses sustained, spatial divided attention and shares some, but not all characteristics of CPTs. The series of experiments described in this thesis contributes to the understanding of the role of the prefrontal cortex and cholinergic modulation in attentional performance; they also highlight differences between the two tasks in behaviour, brain functions and networks. Excitotoxic lesions of the medial prefrontal cortex (mPFC) and a range of cholinergic systemic pharmacology validated the role of the prefrontal cortex and cholinergic modulation in rCPT performance. A chemogenetic study also validated the role of the ascending cholinergic basal forebrain system in 5-CSRTT performance. These findings support 1. the idea of the relationship between cholinergic system activation and attentional performance to resemble an ‘inverted-U’ shaped function; 2. a double dissociation of mPFC sub-regions on attentional performance, in which the prelimbic cortex (PL) appears to play a role in rCPT performance, compared with a role of the anterior cingulate cortex (ACC) in 5-CSRTT performance; and 3. a role of ascending cholinergic projections from the basal forebrain to the ACC in 5-CSRTT performance. These findings also establish the development of a successful flanker distractor probe in rodents on the rCPT. This thesis concludes with an important comparison of the attentional and impulsivity measures in the rCPT compared to the 5-CSRTT, to help provide guidelines as to which task is most appropriate to use for particular research questions.

Subjects/Keywords: attention; cholinergic system; prefrontal cortex; rodent continuous perfromance task

12 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fisher, B. M. (2018). Role of prefrontal cortex and cholinergic modulation in attentional performance in rats. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/274607

Chicago Manual of Style (16^th Edition):

Fisher, Beth Mary. “Role of prefrontal cortex and cholinergic modulation in attentional performance in rats.” 2018. Doctoral Dissertation, University of Cambridge. Accessed January 19, 2021. https://www.repository.cam.ac.uk/handle/1810/274607.

MLA Handbook (7^th Edition):

Fisher, Beth Mary. “Role of prefrontal cortex and cholinergic modulation in attentional performance in rats.” 2018. Web. 19 Jan 2021.

Vancouver:

Fisher BM. Role of prefrontal cortex and cholinergic modulation in attentional performance in rats. [Internet] [Doctoral dissertation]. University of Cambridge; 2018. [cited 2021 Jan 19]. Available from: https://www.repository.cam.ac.uk/handle/1810/274607.

Council of Science Editors:

Fisher BM. Role of prefrontal cortex and cholinergic modulation in attentional performance in rats. [Doctoral Dissertation]. University of Cambridge; 2018. Available from: https://www.repository.cam.ac.uk/handle/1810/274607

North-West University

27. Van Zyl, Petrus Jurgens. Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.

Degree: 2008, North-West University

URL: http://hdl.handle.net/10394/4190

► Despite their acknowledged efficacy, currently available antidepressants still demonstrate undesirable side effects, shortfalls in effectiveness and a delayed onset of action. All these agents act… (more)

▼ Despite their acknowledged efficacy, currently available antidepressants still demonstrate undesirable side effects, shortfalls in effectiveness and a delayed onset of action. All these agents act via monoaminergic mechanisms, although recent studies have begun to note the potential role of the cholinergic system as well as the amino acid pathways in affective isorders. It has been suggested that glutamate NMDA receptor activation may be involved in hippocampal degeneration seen in patients with depression, as well as contributing as a molecular target for the antidepressant action of known antidepressant drugs. Glutamate either separately or via the release of nitric oxide, regulates the release of various transmitters in the brain critical for affective state, e.g. monoamines (noradrenaline, dopamine), indoleamines (5HT), y-aminobutyric acid (GABA) and acetylcholine. The aim of this study was to investigate N-methyl-D-aspartate (I\IMDA) and muscarinic M1 receptor characteristics and also GABA and acetylcholine levels in a genetic animal model of depression, the Flinders Sensitive Line (FSL) rat, with respect to its control, viz. Flinders Resistant Line (FRL) rat, thereby establishing a possible role for the amino acid and cholinergic pathways in the hippocampus and frontal cortex, two brain areas implicated in depression. In addition, anxietylike behaviours were assessed using the open field and social interaction tests. A sensitive liquid chromatography tandem mass spectrometer (LC/MS/MS) method was used in the quantification of acetylcholine as well as high performance liquid chromatography with electrochemical detection (HPLG-EGD) for the quantification of GABA in the above-mentioned brain areas of FSL and FRL rats. NMDA and muscarinic M1 receptor characteristics were expressed in terms of receptor denSity (Bmax) and affinity (Kd) values and were performed using [3H]-MK801 (27.5 Gi/mmol) and quinuclidinyl benzilate (52.0 Gilmmol) for NMDA and M1 receptors, respectively. In addition, to provide evidence for face validity, behavioural assessments were routinely performed using the open field test and social interaction test. Significantly elevated levels of acetylcholine were found in the frontal cortex but with significantly reduced levels in the hippocampus of FSL rats. Cortical and hippocampal muscarinic receptor binding characteristics remained unchanged, while no differences with regard to GABA levels and NMDA receptor binding characteristics were noted in these brain areas. In concordance with studies from the literature, aversive and locomotor behaviour as measured in the open field test, provided evidence of anxiogenic behaviour in the FSL rat, evinced by significantly less social interaction than their FRL counterparts. In addition, evidence for a lack in general activity of the FSL rat in the open field was also noted. Our data therefore suggest the presence of a cholinergic dysfunction in both the frontal cortex and hippocampus of the FSL rat, although this is not accompanied…

Subjects/Keywords: Depression; Flinders sensitive line; Glutamate; GABA; Cholinergic pathway; Frontal cortex; Hippocampus

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van Zyl, P. J. (2008). Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl. (Thesis). North-West University. Retrieved from http://hdl.handle.net/10394/4190

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van Zyl, Petrus Jurgens. “Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl. ” 2008. Thesis, North-West University. Accessed January 19, 2021. http://hdl.handle.net/10394/4190.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van Zyl, Petrus Jurgens. “Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl. ” 2008. Web. 19 Jan 2021.

Vancouver:

Van Zyl PJ. Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl. [Internet] [Thesis]. North-West University; 2008. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10394/4190.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van Zyl PJ. Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl. [Thesis]. North-West University; 2008. Available from: http://hdl.handle.net/10394/4190

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Columbia University

28. Kerk, Sze Yen. Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression.

Degree: 2016, Columbia University

URL: https://doi.org/10.7916/D83B60N6

► A common organizational feature of any nervous system is the existence of groups of neurons that share a set of common traits but that can… (more)

▼ A common organizational feature of any nervous system is the existence of groups of neurons that share a set of common traits but that can be further divided into individual neuron types and subtypes. Understanding the mechanistic basis of neuron type and subtype diversification processes will constitute a major step toward understanding brain development and evolution. In this dissertation, I have explored the mechanistic basis for the specification of motor neuron classes in the nematode C. elegans which serves as a paradigm for neuron diversification processes. Cholinergic motor neurons in the C. elegans ventral nerve cord share common traits, but are also comprised of many distinct classes, each characterized by unique patterns of effector gene expression (e.g. motor neuron class-specific ion channels, signaling molecules, and neurotransmitter receptors). Both the common as well as class-specific traits are directly activated by the terminal selector of cholinergic motor neuron identity, the EBF/COE-like transcription factor UNC-3. Via forward genetic screens to identify mutants that are defective in class specification, I have discovered that the diversification of UNC-3/EBF-dependent cholinergic motor neurons is controlled by distinct sets of phylogenetically conserved, motor neuron class-specific transcriptional repressors. One such repressor is in fact a novel gene previously uncharacterized in C. elegans or any nervous systems and is now named bnc-1. By molecularly dissecting the cis-regulatory region of effector genes, I found that the repressor proteins prevent UNC-3/EBF from activating class-specific effector genes in specific motor neuron subsets via discrete binding sites that are adjacent to those of UNC-3/EBF. And by using CRISPR/Cas9-mediated genome engineering to tag repressor proteins with inducible degrons, I demonstrate that these repressors share the important feature of being continuously required throughout the life of the animal to counteract, in a class-specific manner, the function of the UNC-3/EBF terminal selector that is active in all motor neuron classes. I propose that the strategy of antagonizing the activity of broadly acting terminal selectors of neuron identity in a neuron subtype-specific manner may constitute a general principle of neuron subtype diversification.

Subjects/Keywords: Cholinergic mechanisms; Caenorhabditis elegans; Repressors, Genetic; Neurons; Neurosciences; Genetics

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kerk, S. Y. (2016). Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D83B60N6

Chicago Manual of Style (16^th Edition):

Kerk, Sze Yen. “Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression.” 2016. Doctoral Dissertation, Columbia University. Accessed January 19, 2021. https://doi.org/10.7916/D83B60N6.

MLA Handbook (7^th Edition):

Kerk, Sze Yen. “Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression.” 2016. Web. 19 Jan 2021.

Vancouver:

Kerk SY. Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression. [Internet] [Doctoral dissertation]. Columbia University; 2016. [cited 2021 Jan 19]. Available from: https://doi.org/10.7916/D83B60N6.

Council of Science Editors:

Kerk SY. Diversification of Caenorhabditis elegans motor neuron identity via selective effector gene repression. [Doctoral Dissertation]. Columbia University; 2016. Available from: https://doi.org/10.7916/D83B60N6

29. Kopp, Charles. Cholinergic modulation of excitatory synapses of the ACC and LPFC.

Degree: MS, Medical Sciences, 2017, Boston University

URL: http://hdl.handle.net/2144/23826

► Acetylcholine modulates neuronal activity in the brain with different responses in activity depending on the region of the brain. Our study was focused on the… (more)

Subjects/Keywords: Neurosciences; Cholinergic; Synapse

…Shirke, & Malinow, 1993; Rosenmund, Clements, & Westbrook, 1993) . Cholinergic Signaling… …cortical activation, oscillatory states, and synaptic transmission. Cholinergic fibers in the… …2011). The cholinergic system is essential for learning, memory, and cognition (… …cholinergic receptors in the CNS: nicotinic and muscarinic (Levey, Kitt, Simonds, Price… …1991). The downstream effects of this process have not been described. Cholinergic…

11 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kopp, C. (2017). Cholinergic modulation of excitatory synapses of the ACC and LPFC. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/23826

Chicago Manual of Style (16^th Edition):

Kopp, Charles. “Cholinergic modulation of excitatory synapses of the ACC and LPFC.” 2017. Masters Thesis, Boston University. Accessed January 19, 2021. http://hdl.handle.net/2144/23826.

MLA Handbook (7^th Edition):

Kopp, Charles. “Cholinergic modulation of excitatory synapses of the ACC and LPFC.” 2017. Web. 19 Jan 2021.

Vancouver:

Kopp C. Cholinergic modulation of excitatory synapses of the ACC and LPFC. [Internet] [Masters thesis]. Boston University; 2017. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2144/23826.

Council of Science Editors:

Kopp C. Cholinergic modulation of excitatory synapses of the ACC and LPFC. [Masters Thesis]. Boston University; 2017. Available from: http://hdl.handle.net/2144/23826

University of Western Ontario

30. Major, Alex J. Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task.

Degree: 2019, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/6231

► The ability to flexibly react to our dynamic environment is a cardinal component of cognition and our human identity. Millions across the globe are affected… (more)

▼ The ability to flexibly react to our dynamic environment is a cardinal component of cognition and our human identity. Millions across the globe are affected by disorders of cognition, affecting their ability to live independently. Prefrontal cortex is required for optimal cognitive functioning, but its circuitry is often disrupted in conditions of impaired cognition. In addition, the cholinergic system is vital to optimal executive function, but this is disrupted in a number of conditions, including Alzheimer’s disease and schizophrenia. The actions of cholinergic receptors were explored in this project with local application of cholinergic compounds onto prefrontal neurons as rhesus monkeys performed a rule-based saccadic task that requires working memory maintenance. The antisaccade task is a useful probe of prefrontal cortex function that elicits errors in neuropsychiatric conditions. Some prefrontal neurons respond to different task aspects of the antisaccade task, e.g., discharging preferentially for one task rule over the other (pro- or antisaccades), and are thought to be involved in the circuitry for correct behavioural responses. Chapter 2 explored the effect of general stimulation of cholinergic receptors on rhesus PFC neuronal activity during antisaccade performance. In Chapter 3, newly developed cholinergic receptor subtype-specific compounds were utilized to examine the actions of muscarinic M1 receptor stimulation on prefrontal activity. Cortical oscillations are emerging as an important aspect of cognitive circuitry, such as during working memory maintenance. Chapter 4 examined the influence of local cholinergic receptor stimulation and blockade on the power of local field potential in different frequency bands. This project characterized the role of cholinergic receptors in prefrontal cortical neurons that were actively involved in cognitive circuitry. This and future work on the cholinergic influence on prefrontal cortex will provide insights into the altered cognitive functioning in Alzheimer’s disease and schizophrenia, which are also affected by disrupted cholinergic systems.

Subjects/Keywords: cholinergic; muscarinic; prefrontal cortex; iontophoresis; macaque; working memory; Cognitive Neuroscience

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Major, A. J. (2019). Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Major, Alex J. “Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task.” 2019. Thesis, University of Western Ontario. Accessed January 19, 2021. https://ir.lib.uwo.ca/etd/6231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Major, Alex J. “Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task.” 2019. Web. 19 Jan 2021.

Vancouver:

Major AJ. Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2021 Jan 19]. Available from: https://ir.lib.uwo.ca/etd/6231.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Major AJ. Role of cholinergic receptors in prefrontal activity of nonhuman primates during an oculomotor rule-based working memory task. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6231

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations