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University of Melbourne
1.
KHIEW, HSU WEI.
Cytokines, pain and regulation of inflammation.
Degree: 2015, University of Melbourne
URL: http://hdl.handle.net/11343/55543 
► A mechanism termed the “inflammatory reflex” has been proposed as the basis for autonomic regulation of immune function. The efferent arm of this reflex –…
(more)
▼ A mechanism termed the “inflammatory reflex” has been proposed as the basis for autonomic regulation of immune function. The efferent arm of this reflex – the neural-to-immune link – is thought to be the “cholinergic anti-inflammatory pathway”. According to this concept, an immune challenge is relayed by afferent nerves and/or by humoral signals to the brain, whereupon the brain drives efferent nerve fibres in the vagus that act ultimately to prevent the damaging consequences of excessive inflammation. The cholinergic anti-inflammatory pathway has been extensively studied in vivo and in vitro in terms of its protective effects against a wide range of inflammation-related diseases and immunomodulating functions. However, whether the in vivo anti-inflammatory effect of the cholinergic system can be mirrored and modelled by in vitro culture systems remains controversial and needs to be readdressed.
The reported inhibitory effect of nicotine and the selective cholinergic agonist, AR-R17779, on the disease severity in murine collagen-induced arthritis could not be confirmed. Moreover, in a systematic in vitro study with LPS-stimulated murine macrophage populations and human monocyte/macrophage populations, it was found that nicotine did not reduce tumour necrosis factor (TNF) release even though in vivo it inhibited such expression in splenic macrophages. This suggests caution should be taken in the use of these particular in vitro systems as surrogate assays to study how the cholinergic pathway suppresses systemic inflammation.
Inflammatory pain is multifaceted. Granulocyte macrophage-colony stimulating factor (GM-CSF) and TNF are potential drug targets for inflammatory and pain-related disorders based on the successful translation of animal data into clinical outcomes. In this thesis, TNF and GM-CSF were found to induce pain in an inflammatory footpad model in mice. It was found that neutrophil infiltration was required for TNF- and GM-CSF-induced pain. Colony stimulating factor-1 receptor signaling in macrophages was also required for inflammatory pain. In addition, TNF- and GM-CSF-induced pain was found to be abrogated in B6-KitW-sh/W-sh mice suggesting mast cell involvement. Ion channels, such as TRPV1, TRPA1, NaV1.7, and the neuropeptides, substance P and calcitonin gene-related peptide, were also found to be required for TNF- and GM-CSF-induced inflammatory pain development. The requirement for similar cell types and ion channels suggests that the pathways leading to TNF- and GM-CSF-driven pain might be similar and that TNF and GM-CSF may, in fact, be linked in this system.
Utilizing GM-CSF-/- mice and an anti-GM-CSF mAb-based strategy, it was found that GM-CSF is required for TNF-induced pain. Moreover, treatment with anti-TNF mAb abolished GM-CSF-induced pain, indicating that TNF and GM-CSF are interdependent likely causing an amplification of the inflammatory response. Mice that lacked CCL17 were protected from inflammatory pain evoked by TNF and GM-CSF; CCL17 itself was able to induce inflammatory…
Subjects/Keywords: cytokines; pain; inflammation; cholinergic anti-inflammatory pathway
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APA (6th Edition):
KHIEW, H. W. (2015). Cytokines, pain and regulation of inflammation. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/55543
Chicago Manual of Style (16th Edition):
KHIEW, HSU WEI. “Cytokines, pain and regulation of inflammation.” 2015. Doctoral Dissertation, University of Melbourne. Accessed January 19, 2021.
http://hdl.handle.net/11343/55543.
MLA Handbook (7th Edition):
KHIEW, HSU WEI. “Cytokines, pain and regulation of inflammation.” 2015. Web. 19 Jan 2021.
Vancouver:
KHIEW HW. Cytokines, pain and regulation of inflammation. [Internet] [Doctoral dissertation]. University of Melbourne; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/11343/55543.
Council of Science Editors:
KHIEW HW. Cytokines, pain and regulation of inflammation. [Doctoral Dissertation]. University of Melbourne; 2015. Available from: http://hdl.handle.net/11343/55543
2.
V. Alari.
CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.
Degree: 2013, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/215883
► The α7 nicotinic acetylcholine receptor (α7 nAChR) has a key role in the innate immune system’s inflammatory response, as part of “cholinergic anti-inflammatory pathway”: a…
(more)
▼ The α7 nicotinic acetylcholine receptor (α7 nAChR) has a key role in the innate immune system’s
inflammatory response, as part of “
cholinergic anti-
inflammatory pathway”: a process by which acetylcholine from the vagus nerve reduces the release of the pro-
inflammatory cytokine TNFα, thus allowing for a controlled response to infection. The CHRNA7 gene, in humans, is partially duplicated from exon 5-10 and forms an hybrid with four exons (D-A) of a novel gene, FAM7A. This new gene, CHRFAM7A, which is located in the opposite orientation, at 1.6 Mb, from CHRNA7, is not present on every chromosome 15 and a polymorphic variant, in linkage disequilibrium with a 2bp deletion in exon 6, in the same orientation to the CHRNA7 gene, has been described in a cohort of patients with bipolar disorders and schizophrenia. THP-1 monocytic-like cell line expresses only CHRFAM7A, which was down-regulated on treatment with LPS, by a direct transcriptional mechanism reliant on NF-kB. This effect has been confirmed in primary monocytes and macrophages cell cultures, where CHRFAM7A is expressed 200-1000 times more than CHRNA7. Here, the conventional α7 subunit was up-regulated by LPS treatment, thus suggesting the involvement of CHRFAM7A in the regulation of cell surface α7 receptors’ level (a mechanism unique to humans) and the ability of immune cells to respond to acetylcholine, released from the vagus nerve, during infection. This hypothesis seems to be supported by recent works showing that the duplicated form may have a dominant negative effect on the activity of α7 nAChR. Infact, co-expression of CHRFAM7A with α7 results in a significant reduction of the Ach-evoked currents, suggesting the presence of heteromeric non functional receptors at the plasma membrane.
The promoter region that regulates the expression of CHRFAM7A is still unknown. To try to identify and characterize this region, 5'-RACE experiments were carried out to map the CHRFAM7A mRNA 5’UTR. RNA was extracted from three different cell lines: THP-1 cells, primary human macrophages and SHSY5Y neuroblastoma cell line. Multiple transcription start sites were identified, depending on the cell line used, suggesting the existence of alternative promoters. A series of constructs that recapitulate the mapping of the CHRFAM7A regulatory region, according to the transcription start sites identified, was also generated. They were cloned into a reporter vector and their functionality was tested by transient transfection both in THP-1 and SHSY5Y cell models. Through these experiments, an intronic region (-702/-208 bp from ATG codon, in exon B) and an Alu sequence (-1155/-821 bp) were identified as negative regulators of reporter gene transcription. Future experiments will allow us to identify other regulatory sites, important for proper CHRFAM7A gene expression in different tissues.
Furthermore, two variants exist for CHRFAM7A gene, due to alternative splicing, that gives rise to two protein products of predicted 36 and 47 KDa, whose function is currently unknown. The N-terminally…
Advisors/Committee Members: tutor:D. Fornasari, coordinatore: A. Gianni, FORNASARI, DIEGO MARIA MICHELE, GIANNI, ALESSANDRO.
Subjects/Keywords: CHRFAM7A; cholinergic anti-inflammatory pathway; CHRNA7; alpha7; Settore BIO/14 - Farmacologia
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APA (6th Edition):
Alari, V. (2013). CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/215883
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Alari, V.. “CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.” 2013. Thesis, Università degli Studi di Milano. Accessed January 19, 2021.
http://hdl.handle.net/2434/215883.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Alari, V.. “CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO.” 2013. Web. 19 Jan 2021.
Vancouver:
Alari V. CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. [Internet] [Thesis]. Università degli Studi di Milano; 2013. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/2434/215883.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Alari V. CARATTERIZZAZIONE MOLECOLARE E FUNZIONALE DEL GENE CHRFAM7A, FORMA DUPLICATA DELLA SUBUNITÀ ALPHA7 DEL RECETTORE NICOTINICO. [Thesis]. Università degli Studi di Milano; 2013. Available from: http://hdl.handle.net/2434/215883
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université de Grenoble
3.
Picq, Chloé.
Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system.
Degree: Docteur es, Neurosciences Neurobiologie, 2012, Université de Grenoble
URL: http://www.theses.fr/2012GRENV037
► IntroductionLa neurostimulation vagale (NSV) à haute fréquence (30 Hz) est utilisée commethérapeutique de certaines formes d’épilepsie et de dépression réfractaires aux traitements chezl’Homme. De plus,…
(more)
▼ IntroductionLa neurostimulation vagale (NSV) à haute fréquence (30 Hz) est utilisée commethérapeutique de certaines formes d’épilepsie et de dépression réfractaires aux traitements chezl’Homme. De plus, la NSV à basse fréquence (5 Hz) a été expérimentée avec succès chez l’animalpour traiter différentes inflammations périphériques, notamment digestives. Des travaux récents ontmis en évidence que cet effet
anti-inflammatoire est induit par l’activation des fibres efférentesvagales, libérant en périphérie de l’acétylcholine, inhibant la sécrétion des cytokines proinflammatoires.Cette voie est connue sous le nom de voie
anti-inflammatoire cholinergique.Toutefois, le mécanisme d’action de la NSV 5 Hz reste mal connu et d’autres voies pourraient êtremises en jeu impliquant le système nerveux central (SNC) et le système immunitaire périphérique.ButLes travaux réalisés ont eu pour objectif d’étudier l’implication du SNC et du systèmeimmunitaire dans la modulation de l’inflammation induite par la NSV basse fréquence chez unmodèle de rat anesthésié. Tout d’abord, afin d’étudier l’implication du SNC lors de la NSV 5 Hz, uneétude d’imagerie par résonance magnétique fonctionnelle (IRMf) a été réalisée sur le rat «sain»anesthésié. Ensuite, une étude a été effectuée sur l’effet de la NSV sur les cellules immunitairesspléniques ainsi que sur le tube digestif chez un modèle de rat «sain» puis chez un modèle de ratatteint d’une colite expérimentale induite par une injection intra-colique d’acide trinitrobenzènesulfonique (TNBS).RésultatsLes données obtenues lors de l’étude d’IRMf ont mis en évidence un rôle important desfibres afférentes vagales; elles modulent certaines structures du SNC qui pourraient participer à larégulation de l’inflammation digestive induite par la NSV 5 Hz. Les études réalisées sur les souspopulationslymphocytaires spléniques ont révélé que d’autres cellules immunitaires que lesmacrophages étaient impliquées lors de la NSV. Chez le modèle de rat «sain», les résultats decytométrie en flux ont montré que la NSV 3h 5 Hz induisait une diminution de l’activation deslymphocytes T CD4 ainsi que du pourcentage de NKT par rapport aux lymphocytes T. Ces résultatssont en faveur d’un rôle de la NSV 3h inhibant l’activation lymphocytaire et jouant un rôle sur les NKTpossédant des propriétés immunorégulatrices. La NSV 3h n’a pas le même effet chez le modèle de ratprésentant une colite. En effet, le dosage de cytokines sécrétées par les splénocytes en culturemontre que la NSV augmente le potentiel de sécrétion d’IL-10 (cytokine
anti-inflammatoire) dessplénocytes et plus particulièrement des lymphocytes T CD4 spléniques. Parallèlement, l’effet antiinflammatoirede la NSV a été mis en évidence au niveau du côlon transverse (au-dessus de la zonelésée) par une diminution des ARNm de SOCS3 et du TNF-α et de la myéloperoxidase. Ces donnéesont démontré un rôle de la NSV sur la fonctionnalité des lymphocytes T CD4 spléniques. La NSV 3h 5Hz orienterait la réponse immunitaire vers une réponse
anti-inflammatoire en phase…
Advisors/Committee Members: Bonaz, Bruno (thesis director), Clarençon, Didier (thesis director).
Subjects/Keywords: Nerf vague; Neurostimulation vagale; Voie anti-inflammatoire cholinergique; Voie anti-inflammatoire cholinergique; Inflammation; Maladies inflammatoires chroniques de l'intestin; Neuro-immunologie; Vagus nerve; Vagus nerve stimulation; Cholinergic anti-inflammatory pathway; Inflammation; Chronic inflammatory bowel diseases; Neuro-immunology
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APA ·
Chicago ·
MLA ·
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CSE |
Export
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Manager
APA (6th Edition):
Picq, C. (2012). Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system. (Doctoral Dissertation). Université de Grenoble. Retrieved from http://www.theses.fr/2012GRENV037
Chicago Manual of Style (16th Edition):
Picq, Chloé. “Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system.” 2012. Doctoral Dissertation, Université de Grenoble. Accessed January 19, 2021.
http://www.theses.fr/2012GRENV037.
MLA Handbook (7th Edition):
Picq, Chloé. “Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system.” 2012. Web. 19 Jan 2021.
Vancouver:
Picq C. Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system. [Internet] [Doctoral dissertation]. Université de Grenoble; 2012. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2012GRENV037.
Council of Science Editors:
Picq C. Relations neurodigestives et stimulation vagale basse-fréquence chez le rat anesthésié : implications du système nerveux central et du système immunitaire : Brain-gut interactions and low-frequency vagus nerve stimulation in an anaesthetized rat model : involvement of the central nervous system and the immune system. [Doctoral Dissertation]. Université de Grenoble; 2012. Available from: http://www.theses.fr/2012GRENV037
4.
Rocha, Juraci Aparecida.
Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental.
Degree: PhD, Cardiologia, 2013, University of São Paulo
URL: http://www.teses.usp.br/teses/disponiveis/5/5131/tde-22012014-122449/
;
► INTRODUÇÃO: A atuação do sistema nervoso parassimpático em células imunes é conhecida como \"Via Anti-inflamatória Colinérgica\". Trabalhos prévios demonstraram que a estimulação vagal reduz a…
(more)
▼ INTRODUÇÃO: A atuação do sistema nervoso parassimpático em células imunes é conhecida como \"Via
Anti-inflamatória Colinérgica\". Trabalhos prévios demonstraram que a estimulação vagal reduz a inflamação e melhora a sobrevida em modelos experimentais com sepse. Neste estudo avaliamos se o uso do anticolinesterásico piridostigmina: altera o número de linfócitos T (CD4+ e CD8+) convencionais (CD25+Foxp3-) e reguladores (CD25+Foxp3+) no sangue periférico, no baço e no miocárdio; modifica a concentração de citocinas (interleucina 1, interleucina 6, TNFalfa) no miocárdio; e influencia a função ventricular após infarto agudo do miocárdio experimental (IAM) em ratos. MÉTODOS: Utilizamos ratos machos adultos da linhagem Wistar, com peso variando entre 200 e 250 g, divididos em 3 grupos de 20 animais cada: grupo controle (GC), grupo infartado sem tratamento (IC) e grupo infartado tratado com piridostigmina (IP). O infarto agudo do miocárdio (IAM) foi obtido com a técnica da ligadura da artéria coronária esquerda, e o grupo IP recebeu piridostigmina na dose de 40mg/kg/dia na água de beber, iniciada 4 dias antes do IAM. Todos os animais foram submetidos à canulação da artéria femoral no dia seguinte ao IAM para registro das curvas de pressão arterial, e posterior análise dos componentes da variabilidade da freqüência cardíaca (VFC), domínio do tempo (SDNN e RMSSD) e da freqüência (componentes LF e HF); o estudo ecocardiográfico foi realizado no segundo dia pós IAM. No terceiro dia pós IAM, os ratos foram divididos em subgrupos de 10 animais, e sacrificados de forma específica para coleta de materiais: 500 ul de sangue periférico e baço fresco para realização da técnica de citometria de fluxo; ventrículo esquerdo para dosagem de citocinas pela técnica de ELISA; e ventrículo esquerdo para realização de imunohistoquímica. Foram usadas as técnicas padronizadas e de uso corrente nos laboratórios. Os resultados foram avaliados por análise de variância (ANOVA) multifatorial, usando o programa GraphPad Prism com teste post hoc de Tukey. RESULTADOS: O grupo IC comparado ao grupo controle apresentou queda significativa da pressão arterial e aumento da freqüência cardíaca. O grupo IP, comparado ao grupo IC, apresentou maior atividade vagal, caracterizada pela significante redução da FC e aumento da VFC (SDNN, 9,2±1,5 vs 5,2±0,5 p < 0,05). Os parâmetros ecocardiográficos avaliados evidenciaram presença de área hipo/acinética e redução da fração de ejeção do ventrículo esquerdo nos grupos infartados, de igual magnitude. Com relação ao número de linfócitos T, verificamos que o grupo IC, comparado ao grupo controle, apresentou número significativamente menor de linfócitos reguladores (CD25+Foxp3+) no sangue periférico (CD4+: 63,5 ±1,4 vs 70,6 ±3,2%, e CD8+: 68,3 ±1,9 vs 76,1 ± 2,8%). O grupo IP, comparado ao grupo IC, apresentou significativa redução do número de linfócitos T convencionais no sangue periférico (respectivamente, CD4+: 1,5 ±0,2 vs 2,2 ± 0,2 %; CD8+: 1,1 ± 0,1 vs 1,8 ± 0,9%), e no baço houve redução somente do tipo CD4+…
Advisors/Committee Members: Colombo, Fernanda Marciano Consolim.
Subjects/Keywords: Brometo de piridostigmina; Cholinergic anti-inflammatory pathway; Estimulação nervo vago; Infarto do miocárdio; Inflamação/imunologia; Linfócitos T reguladores; Linfócitos-T; Myocardial infarction; Neuroimmunomodulation; Neuroimunomodulação; Piridostigmina; Pyridostigmine; Ratos Wistar; Receptor alpha 7 nicotinico; T-lymphocytes; Vagal nerve stimulation; Via anti-inflamatória colinérgica
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APA ·
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MLA ·
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APA (6th Edition):
Rocha, J. A. (2013). Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5131/tde-22012014-122449/ ;
Chicago Manual of Style (16th Edition):
Rocha, Juraci Aparecida. “Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental.” 2013. Doctoral Dissertation, University of São Paulo. Accessed January 19, 2021.
http://www.teses.usp.br/teses/disponiveis/5/5131/tde-22012014-122449/ ;.
MLA Handbook (7th Edition):
Rocha, Juraci Aparecida. “Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental.” 2013. Web. 19 Jan 2021.
Vancouver:
Rocha JA. Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Jan 19].
Available from: http://www.teses.usp.br/teses/disponiveis/5/5131/tde-22012014-122449/ ;.
Council of Science Editors:
Rocha JA. Interação da atividade autonômica e resposta imunomoduladora na fase aguda do infarto do miocárdio experimental. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/5/5131/tde-22012014-122449/ ;
5.
Mencel, Malwina.
Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response.
Degree: 2014, University of Waterloo
URL: http://hdl.handle.net/10012/8490
► Agrin and neuregulin are neurotrophic molecules well known for their roles at synapses in the peripheral and central nervous systems. The expression of these two…
(more)
▼ Agrin and neuregulin are neurotrophic molecules well known for their roles at synapses in the peripheral and central nervous systems. The expression of these two molecules is not restricted to these sites however, as they are broadly expressed across multiple organ systems. What roles do agrin and neuregulin play within these alternate systems; what is the function of these molecules outside the nervous system? Here I investigate potential roles for agrin and neuregulin in inflammation. Inflammation is an immediate response by innate immune cells, primarily macrophages, to infection and is characterized by the synthesis of pro-inflammatory mediators. The innate immune system possesses multiple redundant mechanisms to locally control inflammation. The neuro-immune axis is one means of control. Often called the cholinergic anti-inflammatory pathway, it acts to regulate local inflammation via nerve-secreted acetylcholine signaling through the homopentameric α7 nicotinic acetylcholine receptors (α7nAChR) present on macrophages.
Both agrin and neuregulin have been independently described to share an intricate relationship with acetylcholine receptors (AChR) in the nervous system. Agrin is best known for its role in AChR aggregation at the neuromuscular junction while neuregulin has related roles in AChR transcription, cell survival, communication and differentiation. Based on the common characteristics of synapses in the nervous and immune systems we were curious to see if agrin and neuregulin played analogous roles on macrophages.
Here we show that agrin and its receptor dystroglycan are expressed on RAW264.7 macrophages. In addition, agrin treated macrophages demonstrate increased endogenous agrin and α7nAChR expression. By blocking α-dystroglycan (α-DG), a receptor for agrin, with an anti-α-DG antibody we further saw a reduction in agrin expression. We also show that agrin is able to aggregate surface α7nAChRs and transmembrane agrin co-localizes with α7nAChRs therein. Agrin appears to induce approximately a 15-fold increase in anti-inflammatory cytokine IL-10 in macrophages but does not increase pro-inflammatory cytokine TNF-α or IL-6 synthesis. Agrin-treated macrophages challenged with LPS, a potent activator of inflammation, exhibit a 57% decrease in IL-6. Macrophages treated with agrin also exhibit a 4-fold increase in STAT3, a regulator of anti-inflammatory action.
The potential anti-inflammatory effects of agrin in the periphery parallel previous work describing the effects of neuregulin in the brain. Previous work completed by our lab suggests a role for neuregulin in augmenting the expression of α7nAChRs on microglia, the macrophages of the brain, but not in peripheral macrophages. Here we show that treatment of LPS challenged microglia with neuregulin produces an 88% decrease in IL-6 and a 33% decrease in TNF-α. These results indicate both agrin and neuregulin are able to induce an increase in α7nAChRs and augment the synthesis of pro- and anti-inflammatory cytokines in their respective systems.…
Subjects/Keywords: Agrin; Neuregulin; acetylcholine receptors; inflammation; cholinergic anti-inflammatory pathway; TNF; IL-6; IL-10
…76
Figure 6. Inflammation is regulated via the cholinergic anti-inflammatory pathway in… …cholinergic anti-inflammatory pathway, crucial for regulating inflammation.
2
From an… …47
3.1.8 Agrin addition induces an increase in anti-inflammatory IL-10 synthesis in… …49
3.1.9 Agrin addition induces an increase in the anti-inflammatory regulator STAT3… …60
4.3 Agrin may have a primarily anti-inflammatory role in macrophages…
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mencel, M. (2014). Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/8490
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mencel, Malwina. “Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response.” 2014. Thesis, University of Waterloo. Accessed January 19, 2021.
http://hdl.handle.net/10012/8490.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mencel, Malwina. “Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response.” 2014. Web. 19 Jan 2021.
Vancouver:
Mencel M. Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response. [Internet] [Thesis]. University of Waterloo; 2014. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10012/8490.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mencel M. Potential Roles for the Neurotrophic Molecules Agrin and Neuregulin in Regulating Aspects of the Inflammatory Response. [Thesis]. University of Waterloo; 2014. Available from: http://hdl.handle.net/10012/8490
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
6.
Grant, Wilmon Francis.
The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate.
Degree: PhD, 2011, Oregon Health Sciences University
URL: doi:10.6083/M4KW5D1C
;
http://digitalcommons.ohsu.edu/etd/599
Subjects/Keywords: Japanese macaque; Fatty acids; Apoptosis; Digestive organs – Innervation; Macaca fuscata; Cholinergic Anti-Inflammatory Pathway; Development; Macaca; Fatty Acids; Apoptosis; Liver – innervation; Overnutrition
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APA ·
Chicago ·
MLA ·
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CSE |
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APA (6th Edition):
Grant, W. F. (2011). The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate. (Doctoral Dissertation). Oregon Health Sciences University. Retrieved from doi:10.6083/M4KW5D1C ; http://digitalcommons.ohsu.edu/etd/599
Chicago Manual of Style (16th Edition):
Grant, Wilmon Francis. “The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate.” 2011. Doctoral Dissertation, Oregon Health Sciences University. Accessed January 19, 2021.
doi:10.6083/M4KW5D1C ; http://digitalcommons.ohsu.edu/etd/599.
MLA Handbook (7th Edition):
Grant, Wilmon Francis. “The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate.” 2011. Web. 19 Jan 2021.
Vancouver:
Grant WF. The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate. [Internet] [Doctoral dissertation]. Oregon Health Sciences University; 2011. [cited 2021 Jan 19].
Available from: doi:10.6083/M4KW5D1C ; http://digitalcommons.ohsu.edu/etd/599.
Council of Science Editors:
Grant WF. The effects of a maternal high fat diet on hepatic innervation in the nonhuman primate. [Doctoral Dissertation]. Oregon Health Sciences University; 2011. Available from: doi:10.6083/M4KW5D1C ; http://digitalcommons.ohsu.edu/etd/599
7.
Nash, Michelle.
Neuregulin’s role in regulating the anti-inflammatory pathway.
Degree: 2009, University of Waterloo
URL: http://hdl.handle.net/10012/4543
► Inflammation can be up-regulated by microglia and macrophages through the release of pro-inflammatory cytokines such as tumour necrosis factor-alpha (TNF-α). Excess production of TNF-α can…
(more)
▼ Inflammation can be up-regulated by microglia and macrophages through the release of pro-inflammatory cytokines such as tumour necrosis factor-alpha (TNF-α). Excess production of TNF-α can lead to a variety of diseases and even tissue necrosis. Recently, the expression of alpha seven acetylcholine receptors (α7AChR) by microglia have been shown to decrease the amount of TNF-α released. This anti-inflammatory pathway has been studied extensively where researchers are able to reduce TNF-α concentration through α7AChR expression and increases in the concentration of its ligand. I have shown that Neuregulin is able to increase the expression of α7AChR in microglia and macrophages.
Using three immortalized cell lines, BV-2, EOC-20 and RAW 264.7, and primary microglial cells harvest from mice I investigated the role that neuregulin plays in the anti-inflammatory process. Neuregulin signals through the ErbB receptors, a family of tyrosine kinase receptors, to facilitate the effects on ACh expression. My results show that ErbB4 is expressed in BV-2, EOC-20 and RAW 264.7 cell lines while ErbB2-4 receptors are expressed in primary microglia. As well, I was able to show that ErbB4 became phosphorylated upon binding to NRG in immortalized cell lines.
Using an Enzyme Linked Immunsorbent Assay to analyze TNF- α concentration in microglia and macrophages, I was able to demonstrate that increased levels of α7AChRs did not result in a reduction in TNF-α concentration. These results showed that NRG is able to increase α7AChRs in microglia and macrophages after the phosphorylation of the ErbB4 receptors. As well, this increase in α7AChR does not relate to a reduction in TNF-α, thus under these experimental conditions does not have an effect on the anti-inflammatory pathway.
Subjects/Keywords: Neuregulin; Inflammation; Anti-inflammatory pathway; microglia
…x29;.
6
1.4 Microglia and the anti-inflammatory pathway
Research has shown that in the… …1.3: Microglia and the anti-inflammatory pathway. Acetylcholine is an
important… …nicotinic anti-inflammatory pathway.” In this pathway, inflammation is
regulated through the… …inflammatory pathway ..8
Figure 1.4 Neuregulins and the ErbB receptors 11… …Microglia have also been shown to be able to participate in this anti-inflammatory
control through…
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Nash, M. (2009). Neuregulin’s role in regulating the anti-inflammatory pathway. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/4543
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nash, Michelle. “Neuregulin’s role in regulating the anti-inflammatory pathway.” 2009. Thesis, University of Waterloo. Accessed January 19, 2021.
http://hdl.handle.net/10012/4543.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nash, Michelle. “Neuregulin’s role in regulating the anti-inflammatory pathway.” 2009. Web. 19 Jan 2021.
Vancouver:
Nash M. Neuregulin’s role in regulating the anti-inflammatory pathway. [Internet] [Thesis]. University of Waterloo; 2009. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10012/4543.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nash M. Neuregulin’s role in regulating the anti-inflammatory pathway. [Thesis]. University of Waterloo; 2009. Available from: http://hdl.handle.net/10012/4543
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
8.
Foucault, Laura.
Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model.
Degree: Docteur es, Sciences de la Vie et de la Santé, 2018, Université François-Rabelais de Tours
URL: http://www.theses.fr/2018TOUR3314
► La neuroinflammation occupe une place essentielle dans la physiopathologie des maladies neurodégénératives. A ce jour, la prise en charge des patients atteints de maladies neurodégénératives…
(more)
▼ La neuroinflammation occupe une place essentielle dans la physiopathologie des maladies neurodégénératives. A ce jour, la prise en charge des patients atteints de maladies neurodégénératives repose sur des traitements symptomatiques. Depuis quelques années, la communauté scientifique a orienté ses travaux de recherche sur la régulation de l’inflammation cérébrale. Dans ce travail de thèse, nous avons mis en évidence les effets potentiellement anti-inflammatoires et neuroprotecteurs induits par un agoniste des récepteurs nicotiniques de l’acétylcholine alpha 7 (α7 nAChR), le PHA 543613, dans un modèle in vivo de neuroinflammation excitotoxique chez le rat. Ces observations nous ont conduits à réaliser une approche mécanistique visant à quantifier l’expression d’une enzyme anti-oxydante de phase II. Par analogie avec la voie cholinergique anti-inflammatoire observée en périphérie, l’activation des α7 nAChR situés sur les cellules gliales a conduit à la surexpression de l’hème oxygénase 1.
Neuroinflammation is a key component of the pathophysiology of neurodegenerative diseases. To date, the management of patients with neurodegenerative diseases is based on symptomatic treatments. In recent years, the scientific community has focused its research on the regulation of the neuroinflammation. In this thesis, we observed the potential anti-inflammatory and neuroprotective effects induced by an alpha 7 nicotinic acetylcholine receptor agonist (α7 nAChR), PHA 543613, in an in vivo neuroinflammatory model. These observations led us to continue our investigations through a mechanistic approach, and more precisely, to quantify the expression of a phase II antioxidant enzyme. By analogy with the peripheral anti-inflammatory cholinergic pathway, the activation of α7 nAChR localised on glial cells leads to the overexpression of heme oxygenase 1.
Advisors/Committee Members: Antier, Daniel (thesis director), Chalon, Sylvie (thesis director).
Subjects/Keywords: Maladies neurodégénératives; Neuroinflammation; Neurodégénérescence; Récepteurs nicotiniques alpha 7; Voie cholinergique anti-inflammatoire; Neurodegenerative diseases; Neuroinflammation; Neurodegeneration; Nicotinic acetylcholine receptors alpha 7; Anti-inflammatory pathway
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Foucault, L. (2018). Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2018TOUR3314
Chicago Manual of Style (16th Edition):
Foucault, Laura. “Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model.” 2018. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 19, 2021.
http://www.theses.fr/2018TOUR3314.
MLA Handbook (7th Edition):
Foucault, Laura. “Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model.” 2018. Web. 19 Jan 2021.
Vancouver:
Foucault L. Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2018. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2018TOUR3314.
Council of Science Editors:
Foucault L. Evaluation des effets potentiels anti-inflammatoires et neuroprotecteurs d'un agoniste des récepteurs nicotiniques de l'acétylcholine alpha 7 dans un modèle in vivo de neuroinflammation chez le rat : Evaluation of the anti-inflammatory and neuroprotective effects of an alpha 7 nicotinic acteylcholine receptor agonistin an in vivo neuroinflammatory rat model. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2018. Available from: http://www.theses.fr/2018TOUR3314
Université Paris-Sud – Paris XI
9.
Kaci, Ghalia.
Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus.
Degree: Docteur es, Biologie, 2012, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2012PA112427
► Les bactéries commensales digestives jouent un rôle primordial dans l’homéostasie épithéliale et la santé de l’hôte, avec notamment un rôle modulateur du système immunitaire. Des…
(more)
▼ Les bactéries commensales digestives jouent un rôle primordial dans l’homéostasie épithéliale et la santé de l’hôte, avec notamment un rôle modulateur du système immunitaire. Des effets bénéfiques dans le traitement des pathologies inflammatoires intestinales ont été caractérisés chez certaines souches de bactéries commensales. La compréhension de ces effets dans le maintien de l’homéostasie intestinale repose sur la connaissance des interactions entre les bactéries, l’épithélium intestinal et le système immunitaire muqueux. Streptococcus salivarius est l’un des premiers colonisateurs de la cavité buccale et du tractus digestif de l’homme. Cette bactérie a été utilisée comme modèle pour rechercher des mécanismes impliquée dans l’homéostasie.La recherche d’interactions entre des souches de l’espèce S. salivarius et les cellules humaines a été réalisée pour caractériser leurs éventuelles propriétés immunomodulatrices. Nous avons montré que les bactéries vivantes et les surnageants de cultures des souches de cette espèce modulent la réponse inflammatoire in vitro via un effet inhibiteur sur l’activation de la voie NF-B dans les cellules épithéliales intestinales (HT-29 et Caco-2) et les monocytes (THP-1). Cette modulation de l’inflammation a été confirmée par la capacité des surnageants bactériens à inhiber la sécrétion d’IL-8 par les cellules épithéliales. Ces surnageants agissent via une étape impliquant IB-, un inhibiteur du facteur NF-B. Ils inhibent la dégradation de la protéine IB- phosphorylée et diminuent ainsi la translocation nucléaire des composants NF-B. Nous avons également identifié et caractérisé un métabolite bactérien présent dans ces surnageants exerçant cette activité anti-inflammatoire. L’utilisation de ce métabolite et son isomère miment in vitro l’effet inhibiteur des surnageants sur l’activation de la voie NF-B dans les cellules épithéliales et les monocytes. Nous avons ainsi caractérisé un métabolite secrété par la bactérie commensale S. salivarius qui est capable d’inhiber une des voies centrales de signalisation impliquée dans la réponse inflammatoire intestinale. Enfin, une capacité anti-inflammatoire de S. salivarius a également été montrée dans un modèle murin d’inflammation digestive dans lequel les bactéries métaboliquement actives ont protégé les animaux de colites induites avec du TNBS. Ces travaux ouvrent la voie pour le développement d’applications thérapeutiques dans le traitement de pathologies inflammatoires de l’intestin basées sur ce composé actif ou l’utilisation de S. salivarius comme probiotique.
Commensal bacteria play a vital role in epithelial homeostasis and host health, including a modulatory role of the immune system. Their beneficial effects in the treatment of inflammatory bowel disease have been characterized in some strains of commensal bacteria. Understanding these effects in maintaining intestinal homeostasis is based on the knowledge of interactions among bacteria, the intestinal epithelium and the mucosal immune system. Streptococcus salivarius is one…
Advisors/Committee Members: Lafforgue, Christine (thesis director).
Subjects/Keywords: Streptococcus salivarius; Voie de signalisation NF-kB; Effet anti- inflammatoire; Cellules épithéliales intestinales; Monocytes; :Streptococcus salivarius; NF-kB signaling pathway; Anti-inflammatory effect; Intestinal epithelial cells; Monocytes
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kaci, G. (2012). Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA112427
Chicago Manual of Style (16th Edition):
Kaci, Ghalia. “Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 19, 2021.
http://www.theses.fr/2012PA112427.
MLA Handbook (7th Edition):
Kaci, Ghalia. “Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus.” 2012. Web. 19 Jan 2021.
Vancouver:
Kaci G. Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Jan 19].
Available from: http://www.theses.fr/2012PA112427.
Council of Science Editors:
Kaci G. Caractérisation des propriétés anti-inflammatoires de souches commensales de Streptococcus salivarius : Characterization of the anti-inflammatory properties of commensal strains of Streptococcus. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA112427
Deakin University
10.
DOBSON, POLLY.
Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation.
Degree: School of Life and Environmental Sciences, 2016, Deakin University
URL: http://hdl.handle.net/10536/DRO/DU:30089039
► This thesis describes the application of a plant enzyme, soybean 15-lipoxygenase-1, in the production of lipid-based mediators of inflammation from omega-3 fatty acids. Several methods…
(more)
▼ This thesis describes the application of a plant enzyme, soybean 15-lipoxygenase-1, in the production of lipid-based mediators of inflammation from omega-3 fatty acids. Several methods for the biosynthesis and characterisation of such compounds have been developed, which have also provided further insight into the catalytic mechanism of the enzyme.
Advisors/Committee Members: Barrow, Colin, Adcock Jacqui.
Subjects/Keywords: inflammatory diseases; Plant enzymes; anti-inflammatory compounds
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
DOBSON, P. (2016). Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30089039
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
DOBSON, POLLY. “Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation.” 2016. Thesis, Deakin University. Accessed January 19, 2021.
http://hdl.handle.net/10536/DRO/DU:30089039.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
DOBSON, POLLY. “Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation.” 2016. Web. 19 Jan 2021.
Vancouver:
DOBSON P. Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation. [Internet] [Thesis]. Deakin University; 2016. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10536/DRO/DU:30089039.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
DOBSON P. Biocatalysis of Soybean 15-Lipoxygenase for the production of lipid mediators of Inflammation. [Thesis]. Deakin University; 2016. Available from: http://hdl.handle.net/10536/DRO/DU:30089039
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
11.
[No author].
An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-02242009-160331/
► Gold compounds have been used for the treatment of rheumatoid arthritis since the mid 20th century as a disease modifying anti-rheumatic drug. Auranofin, an oral…
(more)
▼ Gold compounds have been used for the treatment of
rheumatoid arthritis since the mid 20th century as a disease
modifying
anti-rheumatic drug. Auranofin, an oral
anti-rheumatic
drug, has been used for many years in the treatment of rheumatoid
arthritis (RA). Although the drug has been successful in treating
the symptoms of RA, many patients discontinue its use due to severe
toxicity over long periods of continued treatment. Since the
introduction of auranofin in 1985 there has been no new clinically
approved gold drug. Drug discovery research is directing focus on
overcoming these toxicity problems. Much of the problems related to
the toxicity related to auranofin are due to its lipophilicity. As
a result, three compounds (Asa-fin, Mpta-fin and Pta-fin) with
varying substituents were synthesised and hence the lipophilic-
hydrophilic balance was modulated. All compounds including
auranofin were tested against normal cells to determine its
toxicity as well as its
anti-
inflammatory activity. Three novel
auranofin derivatives were compared to auranofin with regards to
lipophilicity, toxicity and
anti-
inflammatory properties The
lipophilicity of the three compounds were compared to auranofin
using the octanol-water partition coefficient method. All the novel
compounds showed variable lipophilicity compared to auranofin, with
Pta-fin and Mpta-fin being more hydrophilic than auranofin. The
cytotoxicity of these novel gold compounds Asa-fin, Mpta-fin and
Pta-fin were compared to auranofin using primary porcine
hepatocytes and chicken embryo fibroblasts cultures. A metabolic
assay based on the reactivity of
3-[4,5-dimethylyhiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)
with viable cells was done to measure the effect of the drugs on
the growth of cultures. All three novel compounds proved less
toxicity at comparable concentrations in primary porcine
hepatocytes and in fibroblast proliferation, Asa-fin and Mpta-fin
proved less toxic than Auranofin. The
Anti-
inflammatory activity of
the experimental compounds was determined by testing the effects of
the experimental compounds on human lymphocyte proliferation. The
MTT assay was used to measure the effect of the drugs on the growth
of the cell cultures. All three compounds inhibited the
proliferation of human lymphocytes with Pta-fin having the least
effect. The effect of these drugs was also evaluated on the
reactive oxidant production by chemiluminescence and flow cytometry
on resting, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP)
and Phorbol Myristate Acetate (PMA) stimulated human neutrophils.
Oxidant production by neutrophils was measured after a 45-minute
incubation period with luminol enhanced chemiluminescence.
Treatment of neutrophils with auranofin and the three compounds
showed that auranofin, Asa-fin and Mpta-fin had a biphasic activity
on hydrogen peroxide production with higher concentrations
decreasing hydrogen peroxide production, possibly leading to the
anti-
inflammatory action of these drugs. With Pta-fin no decrease
in hydrogen peroxide was…
Advisors/Committee Members: Prof C E Medlen (advisor).
Subjects/Keywords: Anti-inflammatory;
Cytotoxicity;
Auranofin;
UCTD
Record Details
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Share »
Record Details
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Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2009). An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-02242009-160331/
Chicago Manual of Style (16th Edition):
author], [No. “An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
.” 2009. Masters Thesis, University of Pretoria. Accessed January 19, 2021.
http://upetd.up.ac.za/thesis/available/etd-02242009-160331/.
MLA Handbook (7th Edition):
author], [No. “An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
.” 2009. Web. 19 Jan 2021.
Vancouver:
author] [. An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Jan 19].
Available from: http://upetd.up.ac.za/thesis/available/etd-02242009-160331/.
Council of Science Editors:
author] [. An evaluation of the anti-inflammatory activity and
mechanism of action of three novel auranofin
derivatives
. [Masters Thesis]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-02242009-160331/
University of Pretoria
12.
Rasool, Yusuf.
An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives.
Degree: Pharmacology, 2009, University of Pretoria
URL: http://hdl.handle.net/2263/22792
► Gold compounds have been used for the treatment of rheumatoid arthritis since the mid 20th century as a disease modifying anti-rheumatic drug. Auranofin, an oral…
(more)
▼ Gold compounds have been used for the treatment of
rheumatoid arthritis since the mid 20th century as a disease
modifying
anti-rheumatic drug. Auranofin, an oral
anti-rheumatic
drug, has been used for many years in the treatment of rheumatoid
arthritis (RA). Although the drug has been successful in treating
the symptoms of RA, many patients discontinue its use due to severe
toxicity over long periods of continued treatment. Since the
introduction of auranofin in 1985 there has been no new clinically
approved gold drug. Drug discovery research is directing focus on
overcoming these toxicity problems. Much of the problems related to
the toxicity related to auranofin are due to its lipophilicity. As
a result, three compounds (Asa-fin, Mpta-fin and Pta-fin) with
varying substituents were synthesised and hence the lipophilic-
hydrophilic balance was modulated. All compounds including
auranofin were tested against normal cells to determine its
toxicity as well as its
anti-
inflammatory activity. Three novel
auranofin derivatives were compared to auranofin with regards to
lipophilicity, toxicity and
anti-
inflammatory properties The
lipophilicity of the three compounds were compared to auranofin
using the octanol-water partition coefficient method. All the novel
compounds showed variable lipophilicity compared to auranofin, with
Pta-fin and Mpta-fin being more hydrophilic than auranofin. The
cytotoxicity of these novel gold compounds Asa-fin, Mpta-fin and
Pta-fin were compared to auranofin using primary porcine
hepatocytes and chicken embryo fibroblasts cultures. A metabolic
assay based on the reactivity of
3-[4,5-dimethylyhiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)
with viable cells was done to measure the effect of the drugs on
the growth of cultures. All three novel compounds proved less
toxicity at comparable concentrations in primary porcine
hepatocytes and in fibroblast proliferation, Asa-fin and Mpta-fin
proved less toxic than Auranofin. The
Anti-
inflammatory activity of
the experimental compounds was determined by testing the effects of
the experimental compounds on human lymphocyte proliferation. The
MTT assay was used to measure the effect of the drugs on the growth
of the cell cultures. All three compounds inhibited the
proliferation of human lymphocytes with Pta-fin having the least
effect. The effect of these drugs was also evaluated on the
reactive oxidant production by chemiluminescence and flow cytometry
on resting, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP)
and Phorbol Myristate Acetate (PMA) stimulated human neutrophils.
Oxidant production by neutrophils was measured after a 45-minute
incubation period with luminol enhanced chemiluminescence.
Treatment of neutrophils with auranofin and the three compounds
showed that auranofin, Asa-fin and Mpta-fin had a biphasic activity
on hydrogen peroxide production with higher concentrations
decreasing hydrogen peroxide production, possibly leading to the
anti-
inflammatory action of these drugs. With Pta-fin no decrease
in hydrogen peroxide was…
Advisors/Committee Members: Prof C E Medlen (advisor).
Subjects/Keywords: Anti-inflammatory;
Cytotoxicity;
Auranofin;
UCTD
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APA (6th Edition):
Rasool, Y. (2009). An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/22792
Chicago Manual of Style (16th Edition):
Rasool, Yusuf. “An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives.” 2009. Masters Thesis, University of Pretoria. Accessed January 19, 2021.
http://hdl.handle.net/2263/22792.
MLA Handbook (7th Edition):
Rasool, Yusuf. “An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives.” 2009. Web. 19 Jan 2021.
Vancouver:
Rasool Y. An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives. [Internet] [Masters thesis]. University of Pretoria; 2009. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/2263/22792.
Council of Science Editors:
Rasool Y. An evaluation of
the anti-inflammatory activity and mechanism of action of three
novel auranofin derivatives. [Masters Thesis]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/22792
13.
Nakka, Mamatha.
Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -.
Degree: Chemistry, 2011, Jawaharlal Nehru Technological University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/4496
► CHAPTER 1 A Brief Overview of Synthesis and Pharmacological newlineActivities of Substituted Hydrazones, Sulphonates and Sulphonamides This chapter deals with general survey of the literature…
(more)
▼ CHAPTER 1 A Brief Overview of Synthesis and
Pharmacological newlineActivities of Substituted Hydrazones,
Sulphonates and Sulphonamides This chapter deals with general
survey of the literature related to the present topic which
describes pharmacological importance, synthesis and the synthetic
utility of substituted hydrazones, sulphonates and sulphonamides.
N-acyl hydrazones which are pharmacologically important has been
combined with Non-steroidal anti-inflammatory drugs such as
Naproxen and Ibuprofen drugs used for the treatment of inflammation
and also used as an antipyretic agents. There are conditions when
an inflammation occurs in response to a microbial infection, and a
combination of the anti-inflammatory drug with antimicrobial agent
is prescribed in such conditions. These combinations often cause
side effects because of high doses of drugs. Searching for new
compounds, which would combine two activities seem to be promising
way to overcome that problem. Sulfonates and sulfonamides as we
know that these are the main drugs which are used as antibacterial
agents. In light of theseobservations, we became interested in the
synthesis, characterization and biological evaluation of hybrid
molecules related to N-acyl hydrazones, sulfonates and
sulfonamides. In order to assess more accurately and to provide a
background frame work for the work described in this thesis, a
survey was undertaken of the relevant literature. The details of
synthesis and phamacological activity were presented in this
chapter.CHAPTER 2 Synthesis, characterization, cytotoxic and
antibacterial study of Naproxen derivatives This chapter deals with
a detailed study of synthesis, characterization and cytotoxicity
activity propionic acid based hybrid molecules related to Naproxen
(1) and N acylhydrazones (Scheme 1). Pharmacological importance of
N-acylhydrazones and the gastro intestinal side effects of Naproxen
prompted us to plan the synthesis of new derivatives containing
both the skeletons.
References given chapters wise, Summary
p.287-301
Advisors/Committee Members: Mukkanti, K.
Subjects/Keywords: anti inflammatory drugs; Chemistry
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APA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nakka, M. (2011). Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -. (Thesis). Jawaharlal Nehru Technological University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/4496
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nakka, Mamatha. “Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -.” 2011. Thesis, Jawaharlal Nehru Technological University. Accessed January 19, 2021.
http://shodhganga.inflibnet.ac.in/handle/10603/4496.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nakka, Mamatha. “Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -.” 2011. Web. 19 Jan 2021.
Vancouver:
Nakka M. Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -. [Internet] [Thesis]. Jawaharlal Nehru Technological University; 2011. [cited 2021 Jan 19].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4496.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nakka M. Synthesis, characterization and biological activities of
novel hybrid molecules related to substituted hydrazones,
sulphonamides and sulphonates of non-steroidal anti inflammatory
drugs; -. [Thesis]. Jawaharlal Nehru Technological University; 2011. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/4496
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
Wang, Chenxuan 1991-.
Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress.
Degree: 2018, University of Saskatchewan
URL: http://hdl.handle.net/10388/9654
► Natural polyphenols have been considered as promising anti-aging compounds not only for their antioxidant activity, many of them also function as signaling mediators that modulate…
(more)
▼ Natural polyphenols have been considered as promising
anti-aging compounds not only for their antioxidant activity, many of them also function as signaling mediators that modulate cellular pathways involved in cytoprotection. Avenanthramides (Avns), a group of polyphenols found exclusively in oats, are natural antioxidants associated with human health promotion. In this study, avenanthramide A, B and C (Avn A, B and C), the three most abundant Avns in oats, were identified and quantified from oat phenolic-rich extracts of ten oat varieties. In addition, in vitro antioxidant activities of oat extracts and Avn A, B and C were evaluated. It was found that Avn C had the highest in vitro antioxidant activity among the three Avns. To investigate the cytoprotective activity of Avn C, normal human skin fibroblasts (2DD) were treated with Avn C followed by exposure to extracellular stress and its ability to reduce cellular damage was determined. Pre-treatment of cells with Avn C reduced hydrogen peroxide (H2O2)-induced oxidative stress significantly as demonstrated by decreased intracellular free radical levels and antioxidant gene transcripts. Avn C pre-treatment also resulted in decreased levels of gene transcripts encoding pro-
inflammatory cytokines in response to H2O2 or tumor necrosis factor α (TNF-α) stimulation. This reduction in cytokine gene transcription occurred concomitantly with reduced phosphorylated nuclear factor-κB (NF-κB) p65, indicating reduced pro-
inflammatory response. To better understand the mechanisms of actions, the impact of Avn C on cellular signaling pathways was investigated on Avn C-treated 2DD cells without exposure to stress. We found that Avn C induced heme oxygenase-1 (HO-1) expression through increased DNA-Nrf2 binding activity. Also, it reduced basal levels of pro-
inflammatory cytokines through decreased DNA-NF-κB binding activity. Those mechanism pathways are independent of free radical scavenging and strongly associated with oxidation, inflammation and the aging process. Moreover,
anti-proliferative effect of Avn C on 2DD cells was observed via mechanisms independent of autophagy activation. Collectively, our findings suggest that Avn C protects normal human skin fibroblasts against oxidative stress and
inflammatory response through Nrf2/HO-1 activation and NF-κB inhibition and further imply that Avn C may be a potential nutraceutical for health promotion and disease prevention.
Advisors/Committee Members: Eskiw, Christopher, Qiu, Xiao, Tanaka, Takuji, Low, Nicholas, Yang, Jian.
Subjects/Keywords: Avenathramide C; Antioxidant; Anti-inflammatory
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, C. 1. (2018). Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/9654
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wang, Chenxuan 1991-. “Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress.” 2018. Thesis, University of Saskatchewan. Accessed January 19, 2021.
http://hdl.handle.net/10388/9654.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wang, Chenxuan 1991-. “Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress.” 2018. Web. 19 Jan 2021.
Vancouver:
Wang C1. Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress. [Internet] [Thesis]. University of Saskatchewan; 2018. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10388/9654.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wang C1. Cytoprotective Roles of Oat Avenathramides Against Inflammation and Cellular Stress. [Thesis]. University of Saskatchewan; 2018. Available from: http://hdl.handle.net/10388/9654
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Wake Forest University
15.
West-Livingston, Lauren Nicole.
Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration.
Degree: 2020, Wake Forest University
URL: http://hdl.handle.net/10339/96825
► Cardiovascular disease (CVD) is the leading cause of death globally, and is responsible for innumerable complications related to the peripheral vascular system. Peripheral vascular disease…
(more)
▼ Cardiovascular disease (CVD) is the leading cause of death globally, and is responsible for innumerable complications related to the peripheral vascular system. Peripheral vascular disease and vascular trauma are major contributors toward the need of feasible, effective surgical interventions for vascular repair. While autologous vessels are the gold standard for treatment, synthetic alternatives are necessary for some populations of patients. These synthetic vascular conduits are severely limited and suffer from intimal hyperplasia and loss of patency, requiring intervention relatively soon after the initial surgery.
Subjects/Keywords: Anti-Inflammatory Drug Loading
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
West-Livingston, L. N. (2020). Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/96825
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
West-Livingston, Lauren Nicole. “Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration.” 2020. Thesis, Wake Forest University. Accessed January 19, 2021.
http://hdl.handle.net/10339/96825.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
West-Livingston, Lauren Nicole. “Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration.” 2020. Web. 19 Jan 2021.
Vancouver:
West-Livingston LN. Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration. [Internet] [Thesis]. Wake Forest University; 2020. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10339/96825.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
West-Livingston LN. Development of a Multifunctionalized Vascular Scaffolding System to Induce In Situ Endothelial Cell Capture, Smooth Muscle Cell Migration, and Prevention of Inflammatory Cell Infiltration. [Thesis]. Wake Forest University; 2020. Available from: http://hdl.handle.net/10339/96825
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of KwaZulu-Natal
16.
Nkosi, Makhipha Johannes.
Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital.
Degree: 2015, University of KwaZulu-Natal
URL: http://hdl.handle.net/10413/14951
► Background: Ibuprofen, diclofenac and aspirin belong to the same class of drugs called NSAIDs, but are used interchangeably at Tonga Hospital. The problem with this…
(more)
▼ Background: Ibuprofen, diclofenac and aspirin belong to the same class of drugs called NSAIDs, but
are used interchangeably at Tonga Hospital. The problem with this approach is that it may lead to
preventable misguided and increased spending on pharmaceuticals.
Aim: To investigate the pharmacoeconomic implications of interchangeable use of oral NSAIDs for
pain management at a district hospital.
Setting: This study was conducted at a district hospital in the Tonga village in the Nkomazi
municipality, Mpumalanga Province, South Africa.
Methods: A quantitative retrospective descriptive study, using existing patient records as well as
medicine stock control records, was conducted to investigate the cost-effectiveness of oral NSAIDs
when used interchangeably in the management of pain in adult patients at a district hospital.
Results: The total number of patients included in this study was 211 in a split of 104 in 2013 and 107
in 2014. The mean ages of all the patients who participated in our study in 2013 and 2014 were 36 and
35 years respectively and there were more females than males. Most patients who presented at Tonga
Hospital for pain management were suffering from minor bodily/joint pains (36.0%, n=76), whereas
the least number of patients were suffering from bone fractures (10.9%, n=23). Our study found that
most patients (31.3%; n=66) treated with ibuprofen were suffering from minor bodily/joint pains,
whereas the least number of patients (1.4%; n=3) treated with diclofenac were suffering from
inflammatory conditions. Females were the largest users of NSAIDs (both ibuprofen and diclofenac)
in Tonga Hospital when compared with males. Patients between the ages of 19-35 years were the
most prevalent (28.4%, n=60) who were treated with ibuprofen when compared with patients 18 years
and below (9.5%, n=20). Also, patients who were 18 years and younger and treated with diclofenac
were the least number of patients (1.4%, n=3). The highest total NSAID stock volumes issued from
July to December of 2013 and 2014 combined was ibuprofen (36978 packs) when compared with
diclofenac (11127 packs). The stock volumes for both ibuprofen and diclofenac were higher in July,
with 8170 for Ibuprofen and 2099 for diclofenac. Diclofenac stock volumes fell to their lowest (1583)
in September, whereas Ibuprofen stock volumes fell to their lowest (4478) in December. The fall in stock volumes issued might be attributable to many factors including but not limited to non-delivery
by the supplier or non-ordering by the pharmacy staff. In all instances ibuprofen stock volumes issued
were higher than that of diclofenac. The acquisition cost of ibuprofen when calculated as mean price
per tablet during 2013 to 2014 was consistently lower, with an average price of (0.285 ZAR) when
compared with diclofenac (0.995 ZAR). 0.3% of the population of Nkomazi east, on average, gets
treatment of ibuprofen daily whereas 0.01% of the population gets treatment of diclofenac daily. The
mean cost per defined daily dose was…
Advisors/Committee Members: Mathibe, Lehlohonolo J. (advisor).
Subjects/Keywords: Anti-inflammatory agent.; NSAIDS pharmacology.
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nkosi, M. J. (2015). Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/14951
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nkosi, Makhipha Johannes. “Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital.” 2015. Thesis, University of KwaZulu-Natal. Accessed January 19, 2021.
http://hdl.handle.net/10413/14951.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nkosi, Makhipha Johannes. “Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital.” 2015. Web. 19 Jan 2021.
Vancouver:
Nkosi MJ. Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital. [Internet] [Thesis]. University of KwaZulu-Natal; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10413/14951.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nkosi MJ. Pharmacoeconomic implications of interchangeable use of oral NSAIDS for pain management at a district hospital. [Thesis]. University of KwaZulu-Natal; 2015. Available from: http://hdl.handle.net/10413/14951
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
17.
Bebal, Brenda.
The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
.
Degree: 2015, California State University – San Marcos
URL: http://hdl.handle.net/10211.3/140891
► Statement of Problem Persons who suffer from arthritis have chronic and extensive inflammation, pain and joint damage. Current conventional pharmacological treatment halts the progression of…
(more)
▼ Statement of Problem
Persons who suffer from arthritis have chronic and extensive inflammation, pain and joint damage. Current conventional pharmacological treatment halts the progression of joint
damage, decreases inflammation and pain but may have devastating side effects. This is the impetus to consider alternative intervention that has lesser side effects but can yield similar caliber of reducing inflammation and subsequent pain.
Sources of Data
Three databases were searched to evaluate research studies fitting the inclusion criteria of curcumin therapy to reduce inflammation in arthritis. Eight articles were selected for systematic review. Studies reviewed included meta-analyses, previous systematic reviews, randomized controlled trials, cross- sectional survey studies and controlled trials.
Conclusions Reached
Curcumin has multiple molecular targets and good potential as therapeutic agent for various
inflammatory conditions. The
inflammatory process has a major role in most chronic diseases such as arthritis. Studies support Curcumin as an intervention to decrease inflammation and may offer an alternative for patients with arthritis.
Advisors/Committee Members: Hinchberger, Patricia (advisor).
Subjects/Keywords: Curcumin;
Curcuma longa;
Anti- inflammatory
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bebal, B. (2015). The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
. (Thesis). California State University – San Marcos. Retrieved from http://hdl.handle.net/10211.3/140891
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bebal, Brenda. “The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
.” 2015. Thesis, California State University – San Marcos. Accessed January 19, 2021.
http://hdl.handle.net/10211.3/140891.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bebal, Brenda. “The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
.” 2015. Web. 19 Jan 2021.
Vancouver:
Bebal B. The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
. [Internet] [Thesis]. California State University – San Marcos; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10211.3/140891.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bebal B. The Effects of Curcumin Treatment for Arthritis in Decreasing Inflammation:
A Systematic Review
. [Thesis]. California State University – San Marcos; 2015. Available from: http://hdl.handle.net/10211.3/140891
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rutgers University
18.
Wang, Siyu, 1991-.
Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms.
Degree: PhD, Food Science, 2018, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/59270/
► Over the past few decades, much research has identified chronic inflammation as a critical component in many human diseases. Due to the intimate relationship between…
(more)
▼ Over the past few decades, much research has identified chronic inflammation as a critical component in many human diseases. Due to the intimate relationship between chronic inflammation and human diseases and conditions, an accumulating number of studies aimed to identify and discover
anti-
inflammatory molecules from natural resources because these molecules are able to interact with
inflammatory mediators as well as modulate
inflammatory pathways. Hydroxycinnamic acid amides (HCAA) are the secondary metabolites ubiquitously exist in flowering plants, formed by condensation between hydroxycinnamates and mono or polyamines. HCAA species not only serve multiple functions in plant growth and development, but also exert significant positive effects on human health. HCAA family has been recognized as the most characteristic and abundant chemical species of Lycium barbarum. The plant has been recognized as traditional remedies for hyperglycemia and other health conditions. Wolfberry or Goji berry, the fruits of Lycium barbarum, are widely consumed in Asian cuisine due to their health-promoting properties.
In the first part of my work, we synthesized a set of HCAA compounds, including trans-caffeic acid, trans-ferulic acid, and 3,4-dihydroxyhydrocinnamic acid, with extended phenolic amine components as standards to identify and quantify the corresponding compounds from different parts of Lycium barbarum. With optimized LC–MS/MS and NMR analysis, nine amide compounds were identified from the fruits, and 10 new HCAA species were further identified in root barks in addition to the ones reported in the literature. HCAA species were reported in leaves for the first time. The quantification showed the amide compounds with a tyramine moiety were the most abundant.
Moreover, the method was fully validated with respect to specificity, linearity, intra- and inter-day precision and accuracy, limit of detection (LOD), limit of quantification (LOQ), recovery, and reproducibility.
The
anti-
inflammatory properties of identified HCAAs were also examined by nitric oxide (NO) inhibition assay. Seven HCAA compounds had a potent NO inhibitory effect with IC50 as low as 2.381 μM (trans-N-caffeoyl phenethylamine). Two HCAA compounds (trans-N-caffeoyl tryptamine and trans-N-caffeoyl tyramine) were chosen to investigate their
anti-
inflammatory molecular mechanisms by both in vitro and in vivo assays. These two HCAAs inhibited NF-κB signaling
pathway in murine macrophage RAW264.7, accompanied by inhibition of PI3K/Akt/IKK
pathway. In vivo mouse ear edema model indicated that with treatment of the two HCAAs, TPA-induced ear edema was significant reduced by showing as reduction of ear weight and thickness, pro-
inflammatory enzyme expression as well as immune cells infiltration.
The developed analytical method largely improved analytical sensitivity of HCAAs species that potentially contributes to plant metabolomics and drug discovery studies. HCAAs demonstrated promising
anti-
inflammatory properties that could be used as preventive agent for…
Advisors/Committee Members: HO, CHI-TANG (chair), Wu, Qing-Li (internal member), HUANG, QINGRONG (internal member), Wang, Yu (outside member), School of Graduate Studies.
Subjects/Keywords: Plant metabolites; Anti-inflammatory agents
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, Siyu, 1. (2018). Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/59270/
Chicago Manual of Style (16th Edition):
Wang, Siyu, 1991-. “Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms.” 2018. Doctoral Dissertation, Rutgers University. Accessed January 19, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/59270/.
MLA Handbook (7th Edition):
Wang, Siyu, 1991-. “Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms.” 2018. Web. 19 Jan 2021.
Vancouver:
Wang, Siyu 1. Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Jan 19].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59270/.
Council of Science Editors:
Wang, Siyu 1. Hydroxycinnamic acid amides from Lycium barbarum and their anti-inflammatory mechanisms. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59270/
Rutgers University
19.
Hsieh, Pei-Hsuan, 1988-.
Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage.
Degree: MS, Food Science, 2016, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49239/
► Inflammation is a complicated physiological and pathological process in response to stimuli. In general, a controlled inflammatory response is a beneficial defense system, which provides…
(more)
▼ Inflammation is a complicated physiological and pathological process in response to stimuli. In general, a controlled
inflammatory response is a beneficial defense system, which provides protection against infection. However, it can become detrimental when the response is prolonged and dysregulated called chronic inflammation. Over the past several years, chronic inflammation has been recognized to be the root of various human diseases, including neurological disorders, metabolic disorders, obesity, cardiovascular diseases and cancers. Resveratrol is a well-known natural antioxidative and
anti-
inflammatory compound from grapes. In the previous study, the antioxidative properties of resveratrol metabolites have been suggested to be more active than resveratrol. δ-Viniferin is a main resveratrol dehydrodimer metabolite and has been identified in grape cell cultures and wines. It possesses two resorcinol skeletons, which are determinants of radical scavenging and antioxidative potential. Hence, we researched in the
anti-
inflammatory effect of δ-viniferin. We prepared this compound in vitro by the oxidative dimerization of resveratrol with horseradish peroxidase in the presence of H2O2. In this study, the cytotoxic and
anti-
inflammatory effects on LPS-stimulated murine macrophage were examined. According to the data, we found that δ-viniferin suppressed the levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor κBα (p-IκBα) protein expressions, as well as the down-stream product, nitric oxide, in murine RAW 264.7 cells induced with lipopolysaccharide (LPS). Moreover, the inhibitory effects of δ-viniferin on inflammation associated signaling pathways, such as the blockade of LPS-induced IκBα phosphorylation and degradation, the decrease of PI3K and Akt phosphorylation, and the reduction of LPS-induced transcriptional activity of NF-κB. According to the results, δ-viniferin might have potential to be developed into an effective
anti-
inflammatory agent.
Advisors/Committee Members: HO, CHI-TANG (chair), Hartman, Thomas G (internal member), HUANG, QINGRONG (internal member).
Subjects/Keywords: Anti-inflammatory agents; Resveratrol; Inflammation
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APA (6th Edition):
Hsieh, Pei-Hsuan, 1. (2016). Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49239/
Chicago Manual of Style (16th Edition):
Hsieh, Pei-Hsuan, 1988-. “Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage.” 2016. Masters Thesis, Rutgers University. Accessed January 19, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/49239/.
MLA Handbook (7th Edition):
Hsieh, Pei-Hsuan, 1988-. “Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage.” 2016. Web. 19 Jan 2021.
Vancouver:
Hsieh, Pei-Hsuan 1. Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Jan 19].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49239/.
Council of Science Editors:
Hsieh, Pei-Hsuan 1. Anti-inflammatory effect of resveratrol metabolite, δ-viniferin, on lipopolysaccharide-stimulated murine macrophage. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49239/
University of Otago
20.
Kőszegi, Zsombor.
Restorative effect of estrogen on basal forebrain cholinergic neurons
.
Degree: 2011, University of Otago
URL: http://hdl.handle.net/10523/1887
► The basal forebrain cholinergic (BFC) system is one of the most important neurotransmitter systems in the brain. It has received much attention in the past…
(more)
▼ The basal forebrain
cholinergic (BFC) system is one of the most important neurotransmitter systems in the brain. It has received much attention in the past two decades, primarily for its role in learning, memory, attention and behavior. The BFC system has also been reported to be particularly vulnerable in neurodegenerative diseases, such as in Alzheimer’s disease (AD). The gonadal steroid, estrogen, is an essential contributor in controlling the vulnerability of the BFC system. Besides its classical or genomic mechanism, estrogen is known to have non-classical actions on intracellular signaling pathways. In this study, we investigated the ameliorative effects of estrogen treatment and the role of non-classical estrogen actions on BFC neurons in a neurodegenerative mouse model, in vivo.
N-methyl-D-aspartate (NMDA) was injected unilaterally into the substantia innominata - nucleus basalis magnocellularis (SI-NBM) complex of the basal forebrain to elicit
cholinergic cell death in the injected area and thus fiber loss in the ipsilateral cortex. An acute treatment of 17β-estradiol (E2) after the NMDA-induced lesion restored the ipsilateral
cholinergic fiber density in the cortex in a time- and dose-dependent manner. Conversely, it did not have any effect on the
cholinergic cell loss in the SI-NBM. The ameliorative action of E2 on
cholinergic fiber loss was detected in both intact and gonadectomized young male and female mice, but not in aged animals. The E2-induced
cholinergic fiber density restoration was also absent in neuron-specific estrogen receptor α (ERα) knockout mice. Selective blockade of the mitogen activated protein kinase (MAPK) and protein kinase A (PKA) pathways prevented E2’s ability to restore the
cholinergic fiber density. Furthermore, activation of non-classical estrogen signaling by a non-classical
pathway activator (estren) induced E2-like fiber restoration.
Our findings demonstrate that estrogen restores the
cholinergic fiber density in the cortex through a non-classical signaling mechanism after the loss of subcortical
cholinergic input. Similar restorative effects were observed in young animals, irrespective of sex or endogenous estrogen levels. These observations reveal a critical role for non-classical estrogen signaling via ERα and MAPK-PKA pathways in BFC neurons, in vivo. Taken together, our study discloses important aspects relating to the vulnerability of the BFC system in neurodegenerative processes, such as AD or traumatic brain injury and might shed light on future medical treatments through the use of non-classical estrogen
pathway activators.
Advisors/Committee Members: Ábrahám, István (advisor).
Subjects/Keywords: estrogen;
cholinergic;
basal forebrain;
neurodegeneration;
non-classical;
signaling pathway
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Manager
APA (6th Edition):
Kőszegi, Z. (2011). Restorative effect of estrogen on basal forebrain cholinergic neurons
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/1887
Chicago Manual of Style (16th Edition):
Kőszegi, Zsombor. “Restorative effect of estrogen on basal forebrain cholinergic neurons
.” 2011. Doctoral Dissertation, University of Otago. Accessed January 19, 2021.
http://hdl.handle.net/10523/1887.
MLA Handbook (7th Edition):
Kőszegi, Zsombor. “Restorative effect of estrogen on basal forebrain cholinergic neurons
.” 2011. Web. 19 Jan 2021.
Vancouver:
Kőszegi Z. Restorative effect of estrogen on basal forebrain cholinergic neurons
. [Internet] [Doctoral dissertation]. University of Otago; 2011. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10523/1887.
Council of Science Editors:
Kőszegi Z. Restorative effect of estrogen on basal forebrain cholinergic neurons
. [Doctoral Dissertation]. University of Otago; 2011. Available from: http://hdl.handle.net/10523/1887
North-West University
21.
Van Zyl, Petrus Jurgens.
Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
Degree: 2008, North-West University
URL: http://hdl.handle.net/10394/4190
► Despite their acknowledged efficacy, currently available antidepressants still demonstrate undesirable side effects, shortfalls in effectiveness and a delayed onset of action. All these agents act…
(more)
▼ Despite their acknowledged efficacy, currently available antidepressants still demonstrate undesirable side effects, shortfalls in effectiveness and a delayed onset of action. All these agents act via monoaminergic mechanisms,
although recent studies have begun to note the potential role of the cholinergic system as well as the amino acid pathways in affective isorders.
It has been suggested that glutamate NMDA receptor activation may be involved in hippocampal degeneration seen in patients with depression, as well as contributing as a molecular target for the antidepressant action of
known antidepressant drugs. Glutamate either separately or via the release of nitric oxide, regulates the release of various transmitters in the brain critical for affective state, e.g. monoamines (noradrenaline, dopamine), indoleamines
(5HT), y-aminobutyric acid (GABA) and acetylcholine. The aim of this study
was to investigate N-methyl-D-aspartate (I\IMDA) and muscarinic M1 receptor characteristics and also GABA and acetylcholine levels in a genetic animal model of depression, the Flinders Sensitive Line (FSL) rat, with respect to its
control, viz. Flinders Resistant Line (FRL) rat, thereby establishing a possible role for the amino acid and cholinergic pathways in the hippocampus and frontal cortex, two brain areas implicated in depression. In addition, anxietylike
behaviours were assessed using the open field and social interaction
tests. A sensitive liquid chromatography tandem mass spectrometer
(LC/MS/MS) method was used in the quantification of acetylcholine as well as
high performance liquid chromatography with electrochemical detection
(HPLG-EGD) for the quantification of GABA in the above-mentioned brain
areas of FSL and FRL rats. NMDA and muscarinic M1 receptor
characteristics were expressed in terms of receptor denSity (Bmax) and affinity
(Kd) values and were performed using [3H]-MK801 (27.5 Gi/mmol) and
quinuclidinyl benzilate (52.0 Gilmmol) for NMDA and M1 receptors,
respectively. In addition, to provide evidence for face validity, behavioural assessments were routinely performed using the open field test and social
interaction test. Significantly elevated levels of acetylcholine were found in the frontal cortex but with significantly reduced levels in the hippocampus of FSL rats. Cortical
and hippocampal muscarinic receptor binding characteristics remained
unchanged, while no differences with regard to GABA levels and NMDA
receptor binding characteristics were noted in these brain areas. In
concordance with studies from the literature, aversive and locomotor
behaviour as measured in the open field test, provided evidence of anxiogenic
behaviour in the FSL rat, evinced by significantly less social interaction than
their FRL counterparts. In addition, evidence for a lack in general activity of
the FSL rat in the open field was also noted. Our data therefore suggest the
presence of a cholinergic dysfunction in both the frontal cortex and
hippocampus of the FSL rat, although this is not accompanied…
Subjects/Keywords: Depression;
Flinders sensitive line;
Glutamate;
GABA;
Cholinergic pathway;
Frontal cortex;
Hippocampus
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APA ·
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Manager
APA (6th Edition):
Van Zyl, P. J. (2008). Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
(Thesis). North-West University. Retrieved from http://hdl.handle.net/10394/4190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Van Zyl, Petrus Jurgens. “Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
” 2008. Thesis, North-West University. Accessed January 19, 2021.
http://hdl.handle.net/10394/4190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Van Zyl, Petrus Jurgens. “Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
” 2008. Web. 19 Jan 2021.
Vancouver:
Van Zyl PJ. Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
[Internet] [Thesis]. North-West University; 2008. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10394/4190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Van Zyl PJ. Regional neurochemical characterization of the flinders sensitive line rat with regard to gaba and cholinergic signalling pathways / P.J. van Zyl.
[Thesis]. North-West University; 2008. Available from: http://hdl.handle.net/10394/4190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
22.
Wen, Yi-shan.
An anti-inflammatory activity of marine-derived compound for cosmeceutical use.
Degree: Master, Marine Biotechnology and Resources, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716113-162938
► Most Asians consider fair skin to be a key element of beauty. According to market statistics (source: Euromonitor International), in 2012, cosmetics accounted for a…
(more)
▼ Most Asians consider fair skin to be a key element of beauty. According to market statistics (source: Euromonitor International), in 2012, cosmetics accounted for a revenue of more than $430 billion in global markets. Some of these cosmetics are used for purposes other than beauty; âcosmeceuticalâ applications can be utilized for functions or mechanisms to soothe skin conditions. For example: postinflammatory hyperpigmentation, which usually accompanies disorders associated with melanogenesis, is caused by dermatological diseases such as contact dermatitis and atopic dermatitis or by skin damage due to UV exposure. Some of the widely used skin-whitening agents in cosmetic products or for hyperpigmentation diseases, such as hydroquinone and arbutin, are toxic and, hence, have their limitations. Findings of our preliminary
anti-
inflammatory screening procedure showed that a marine-derived compound significantly inhibited the expression of lipopolysaccharide-induced proinflammatory protein iNOS in murine macrophage-like RAW264.7 cells. The aim of this study was to find a safer and more effective compound than those currently in use, with
anti-
inflammatory,
anti-melanogenic or other applicable properties for future cosmeceutical or medical use. Our results indicated that the marine-derived compound could effectively inhibit pigmentation likely by the potential downregulation of tyrosinase activity; furthermore, the compound also demonstrated low cytotoxic effect dose on B16-F10 murine melanoma cells. We also found that the marine-derived compound could suppress melanin formation while sustaining delivery treatment in the zebrafish model, without having any toxic effects. Results of our in vitro and in vivo biological assays showed that the synthetic marine-derived compound possesses
anti-
inflammatory,
anti-melanogenic and
anti-oxidative properties, with potential application in medical cosmetology.
Advisors/Committee Members: Zhi-Hong, Wen (committee member), Chang-Yi, Wu (chair), Hui-Min, Wang (chair).
Subjects/Keywords: melanin; anti-inflammatory; anti-melanogenic; zebrafish; tyrosinase
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Manager
APA (6th Edition):
Wen, Y. (2013). An anti-inflammatory activity of marine-derived compound for cosmeceutical use. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716113-162938
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wen, Yi-shan. “An anti-inflammatory activity of marine-derived compound for cosmeceutical use.” 2013. Thesis, NSYSU. Accessed January 19, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716113-162938.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wen, Yi-shan. “An anti-inflammatory activity of marine-derived compound for cosmeceutical use.” 2013. Web. 19 Jan 2021.
Vancouver:
Wen Y. An anti-inflammatory activity of marine-derived compound for cosmeceutical use. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716113-162938.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wen Y. An anti-inflammatory activity of marine-derived compound for cosmeceutical use. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716113-162938
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade Estadual de Campinas
23.
Lazarini, Josy Goldoni, 1990-.
Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil.
Degree: 2020, Universidade Estadual de Campinas
URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/339609
► Abstract: Brazilian native fruits (FNB) can be classified as "superfruits" due to their rich polyphenolic composition that can modulate the inflammatory process as well as…
(more)
▼ Abstract: Brazilian native fruits (FNB) can be classified as "superfruits" due to their rich polyphenolic composition that can modulate the
inflammatory process as well as reduce the production of reactive oxygen and nitrogen species (ROS/RNS). This study determined the polyphenolic composition, evaluated the mechanism of
anti-
inflammatory action, antioxidant activity and systemic toxicity profile of the extract, fraction (F3) and subfraction (S8) of Eugenia neonitida (Ene). The compounds present in Ene, F3, and S8 were quantified and identified by LC-ESI-QTOF-MS. For the studies of
anti-
inflammatory activity in vitro, RAW 264.7 macrophages (NF-kB-pLUC gene) transfected were used stimulated with LPS and quantification of
inflammatory cytokines by ELISA. For the evaluation of the
anti-
inflammatory mechanism of action in vivo, neutrophil migration tests were performed in the peritoneal cavity of mice challenged with carrageenan, quantification of
inflammatory cytokines in vivo using ELISA, intravital microscopy (rolling and leukocyte adhesion) and expression of adhesion molecule (ICAM-1) by Western Blotting. For antioxidant activity, tests against ROS/RNS (ROO, O2 -, HOCl and NO ) were used. Finally, the systemic toxicity profile was tested in Galleria mellonella larvae. Chemical analysis revealed the presence of hydroxybenzoic acid, flavanoids and ellagitannins for Ene and F3 and derivatives of quercetrin, vanillic acid and cumaric acid for S8. Ene reduced the activation of NF-kB and TNF-'alfa' levels in vitro, however, it did not reduce CXCL2/MIP-2; Ene treated in vivo decreased neutrophil migration as well as TNF-'alfa' and CXCL2/MIP-2 levels. Regarding the fraction, F3 reduced the activation of NF-kB, the levels of TNF-'alfa' and CXCL2 / MIP-2 (in vitro and in vivo) and migration of neutrophils. Finally, S8 reduced activation of NF-kB, levels of TNF-'alfa' and CXCL2 / MIP-2 (in vitro and in vivo) and neutrophil migration confirmed by intravital microscopy and reduced expression of ICAM-1 (adhesion molecule) in endothelium. In general, Ene, F3 and S8 exhibited activities against ROS/RNS and none of the samples induced systemic toxicity in G. mellonella. Thus, Ene, F3 and S8 showed no toxicity and exibited
anti-
inflammatory and antioxidant activities due to their phenolic composition. E. neonitida is a promising source of bioactive compounds little explored, which can favor sectors such as food industries, pharmaceutical and agribusiness, providing the development of new products for human health
Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Rosalen, Pedro Luiz, 1960- (advisor), Franchin, Marcelo, 1987- (coadvisor), Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba (institution), Programa de Pós-Graduação em Odontologia (nameofprogram), Souza, Ana Paula de (committee member), Dias, Patricia Correa (committee member), Ikegaki, Masaharu (committee member), Tavassi, Ana Marisa Chudzinski (committee member).
Subjects/Keywords: Anti-inflamatórios; Antioxidantes; Anti-inflammatory agents; Antioxidants
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APA (6th Edition):
Lazarini, Josy Goldoni, 1. (2020). Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/339609
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lazarini, Josy Goldoni, 1990-. “Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil.” 2020. Thesis, Universidade Estadual de Campinas. Accessed January 19, 2021.
http://repositorio.unicamp.br/jspui/handle/REPOSIP/339609.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lazarini, Josy Goldoni, 1990-. “Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil.” 2020. Web. 19 Jan 2021.
Vancouver:
Lazarini, Josy Goldoni 1. Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil. [Internet] [Thesis]. Universidade Estadual de Campinas; 2020. [cited 2021 Jan 19].
Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/339609.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lazarini, Josy Goldoni 1. Phytochemical, toxicity, and evaluation of anti-inflammatory and antioxidant activities of "Eugenia neonitida" Sobral (pitangatuba), a Brazilian native fruit : Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil: Fitoquímica, toxicidade e avaliação das atividades anti-inflamatória e antioxidante de "Eugenia neonitida" Sobral (pitangatuba), uma fruta nativa do Brasil. [Thesis]. Universidade Estadual de Campinas; 2020. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/339609
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Sul
24.
Boligon, Caroline Schardong.
Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática.
Degree: 2015, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/132154
► Objetivo: revisar sistematicamente os efeitos da Spirulina platensis em marcadores inflamatórios em humanos. Métodos: foi realizada busca por ensaios clínicos randomizados, realizados em humanos adultos…
(more)
▼ Objetivo: revisar sistematicamente os efeitos da Spirulina platensis em marcadores inflamatórios em humanos. Métodos: foi realizada busca por ensaios clínicos randomizados, realizados em humanos adultos com o objetivo de verificar os efeitos da Spirulina Platensis sob marcadores inflamatórios. A busca foi efetuada até outubro de 2015 nas bases de dados Medline via Pubmed, Cochrane Central, Clinical Trials, Scielo, LILACS, banco de teses da CAPES, assim como em bancos de grey literature. Resultados: Identificados vinte e oito estudos dos quais foram elegíveis três ensaios clínicos randomizados. A amostra foi constituída de 34 a 78 participantes, de ambos os sexos, com idade variando de 18 a 87 anos. Os marcadores inflamatórios avaliados foram: IL-2, IL-6, e TNF- e a intervenção foi feita com cápsulas de Spirulina platensis versus placebo. A IL-2 foi avaliada em dois estudos porém, não foi possível realizar a metanálise, pois um dos artigos não descrevia os valores encontrados em seu grupo controle. A IL-6 ao ser analisada em conjunto, não demonstrou diferença significativa com o uso da Spirulina platensis [-0,36 (IC95%: -0,90 a 0,18), com I2=0%]. Quanto ao TNF- não houve diferença significativa com o uso da Spirulina platensis [-0,03 (IC95%: -0,42 a 0,37)] com teste de inconsistência mostrando moderada heterogeneidade e insignificância estatística [I2= 31%, P= 0,23]. Conclusão: A informação disponível sobre o tema proposto é escassa e o efeito da Spirulina platensis sobre os marcadores inflamatórios se mostrou inconsistente. Ensaios clínicos randomizados adicionais com maior número de participantes e melhores avaliações dos marcadores são necessários para verificar o real papel anti-inflamatório da Spirulina platensis.
Objective: Perform a systematic review on the anti-inflammatory effects of Spirulina platensis in humans. Methods: A search was conducted in Medline via PubMed, Cochrane Central and Clinical Trials until October 2015 and dissertations published in CAPES, and gray literature banks for randomized controlled trials performed in adults to assess the anti-inflammatory effects of Spirulina platensis. Results: The search identified twenty eight articles. Only three randomized clinical trials were elegible for this study. The sample ranged between 34 and 78 participants, both genders, with the age ranged from 18 to 78 years. The measurements of inflammatory outcome were assessed through IL-2, IL-6, and TNF-. The intervention was capsules of Spirulina platensis versus placebo. The IL-2 was evaluated in two studies but it was not possible to evaluate the results together through meta-analysis because one of the articles did not describe the values in the control group. IL-6, when analyzed together, showed no significant difference between Spirulina platensis and placebo [-0.36 (95% CI: -0.90 to 0.18), with I2 = 0%]. Regarding the TNF- no significant difference was found [-0.03 (95% CI: -0.42 to 0.37)]. The inconsistency test showed moderate heterogeneity and no statistical significance [I2 = 31%, P =…
Advisors/Committee Members: Fagundes, Renato Borges.
Subjects/Keywords: Spirulina platensis; Anti-inflamatórios; Spirulina; Anti-inflammatory; Inflammatory markers
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Manager
APA (6th Edition):
Boligon, C. S. (2015). Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/132154
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Boligon, Caroline Schardong. “Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática.” 2015. Thesis, Universidade do Rio Grande do Sul. Accessed January 19, 2021.
http://hdl.handle.net/10183/132154.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Boligon, Caroline Schardong. “Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática.” 2015. Web. 19 Jan 2021.
Vancouver:
Boligon CS. Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2015. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/10183/132154.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Boligon CS. Spirulina platensis e marcadores inflamatórios em humanos : uma revisão sistemática. [Thesis]. Universidade do Rio Grande do Sul; 2015. Available from: http://hdl.handle.net/10183/132154
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
25.
Lin, Chi-Jui.
The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.
Degree: Master, Marine Biotechnology and Resources, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
► New bioactive compounds in microalgae increase their commercial values for healthy food. Nannochloropsis oculata is mostly used for juvenile feed, while application for health is…
(more)
▼ New bioactive compounds in microalgae increase their commercial values for healthy food. Nannochloropsis oculata is mostly used for juvenile feed, while application for health is unavailable so this research isolated the compositions of the
anti-
inflammatory ingredients from N. oculata. The results showed growth of N. oculata yielded well in 110 rpm, 25 °C, 15000 lux, and 2.8% salinity; additional nutrients and CaCO3 slightly increased production. The analysis of N. oculata
anti-
inflammatory ingredients was based on the inhibition ratios of iNOS and COX-2 protein expression in RAW 264.7 under LPS induction. TLC separation showed that highly polar molecules inhibited iNOS and COX-2 expressions. AWP2-11-1 and AWP2-11-3 compounds were from acetone partition. In RP-C18 separation of the extracts, A3-19-21 compound inhibited iNOS and COX-2 expressions by 99% and 95%, respectively. HP2-69-70 compound from n-hexane partition inhibited iNOS expression by 64%. The resulting compounds were identified for their structures via 1H-NMR, 13C-NMR, 2 D NMR, and mass spectrometries.
Advisors/Committee Members: Ping-Jyun Sung (committee member), Zhi-hong, Wen (chair), Chan-Shing Lin (committee member), Ming-Wei Lu (chair), Min-Ying Wang (chair).
Subjects/Keywords: Nannochloropsis oculata; anti-inflammatory; iNOS; COX-2
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lin, C. (2014). The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lin, Chi-Jui. “The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.” 2014. Thesis, NSYSU. Accessed January 19, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lin, Chi-Jui. “The study on anti-inflammatory effects of the extract from Nannochloropsis oculata.” 2014. Web. 19 Jan 2021.
Vancouver:
Lin C. The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lin C. The study on anti-inflammatory effects of the extract from Nannochloropsis oculata. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0522114-222924
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
26.
Wu, Meng-shan.
The effects of anti-inflammatory marine compound on dermatology disorders.
Degree: Master, Marine Biotechnology and Resources, 2016, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0223116-110613
► Skin is a mechanical and a chemical barrier against the invasion of pathogens. In addition, skin also plays an important role in the secretion, temperature…
(more)
▼ Skin is a mechanical and a chemical barrier against the invasion of pathogens. In addition, skin also plays an important role in the secretion, temperature homeostasis, excretion, respiration and skin sensory. Therefore, when skin damage doesnât receive proper treatments, it will result in annoying chronic diseases. In this study, we focus on two different dermatologic disorders which are burn injury and atopic dermatitis. Both of two diseases are very common and high prevalence. Unfortunately, there are no drugs that could effectively treat burn injury and AD without side effects. Thence, developing new drugs for these diseases is imperative. The diversity of marine resources improves marine drugs to generate different bioactivities unlike terrestrial plants. For this reason, ocean may exist a promising hope to treat burn injury and AD. Marine compound (EWH) was extracted from the sea gorgonian. Our previous study also found that EWH has
anti-
inflammatory effects for relieving rheumatoid arthritis and reducing the pro-
inflammatory cytokines. In our study, we used in vitro cell wound healing model to assess whether EWH could promote EA hy 926 endothelial cells and fibroblasts Hs68 migration. Besides, EWH could enhance angiogenesis by using zebrafish model. Next, we found that EWH could improve wound healing of burn injury by rat model and the therapeutic effect is better than silver sulfadiazine cream which is the clinical drug. On the other part, EWH may alleviate the symptoms of AD and reduce the AD score. In a word, EWH may have potential to become a promising drug to treat burn injury and AD in the future.
Advisors/Committee Members: Hui-Min Wang (chair), Zhi-hong, Wen (committee member), Chun-lin Chen (chair).
Subjects/Keywords: Burn; Anti-inflammatory; Angiogenesis; Atopic dermatitis
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wu, M. (2016). The effects of anti-inflammatory marine compound on dermatology disorders. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0223116-110613
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wu, Meng-shan. “The effects of anti-inflammatory marine compound on dermatology disorders.” 2016. Thesis, NSYSU. Accessed January 19, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0223116-110613.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wu, Meng-shan. “The effects of anti-inflammatory marine compound on dermatology disorders.” 2016. Web. 19 Jan 2021.
Vancouver:
Wu M. The effects of anti-inflammatory marine compound on dermatology disorders. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Jan 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0223116-110613.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wu M. The effects of anti-inflammatory marine compound on dermatology disorders. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0223116-110613
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
27.
[No author].
An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
.
Degree: 2008, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-05122008-125709/
► Humin substances have been used as folk remedies for the last 3000 years. Recent studies have shown that humates possess anti-inflammatory properties, but the mechanism…
(more)
▼ Humin substances have been used as folk remedies for
the last 3000 years. Recent studies have shown that humates possess
anti-
inflammatory properties, but the mechanism of how it affects
inflammation is still unclear. In this study the
anti-
inflammatory
properties of potassium humate, a water soluble humic acid salt,
was investigated on different
inflammatory pathways in vitro and in
vivo. The effect of potassium humate on human mononuclear
lymphocyte proliferation showed that potassium humate stimulated
lymphocyte proliferation of resting-, PHA- and PWM-stimulated
lymphocytes in vitro from concentrations of 20 to 80 µg/ml, in a
dose dependant manner, where a maximum proliferation was observed
at 80 µg/ml whereas lymphocyte proliferation decreased at 100
µg/ml. On the contrary potassium humate, at 40 µg/ml, significantly
inhibited the supernatant concentrations of the following
cytokines; TNF-α, IL-1ß, IL-6 and IL-10 by PHA stimulated
lymphocytes. The effect of potassium humate on the alternative as
well as the classical complement
pathway was investigated in vitro
using the haemolytic complement assay. Results indicated that
potassium humate inhibits both the alternative and classical
complement pathways without affecting the red blood cell membrane
stability. Different
inflammatory mechanisms were investigated in
vivo, using the carrageenan-induced paw oedema model and the
delayed type hypersensitivity reaction model. The
carrageenan-induced paw oedema model was used to determine the
effect of potassium humate on acute inflammation in the hind paw.
Carrageenan was injected into the right hind footpad of a rat which
caused an increase in paw volume due to oedema, which was measured
with a plethysmometer. Potassium humate significantly inhibited the
oedema at a dose of 60 mg/kg bodyweight and compared favourably
with indomethacin at 10 mg/kg bodyweight. The effect of potassium
humate on the delayed type hypersensitivity reaction model was also
investigated whereby rats were sensitised with sheep erythrocytes.
Potassium humate was administered daily by oral gavage at a dose of
60 mg/kg bodyweight. After 7 days, rats were challenged by
injecting sheep erythrocytes into the right hind footpad. The
degree of inflammation was determined by measuring the increase of
paw volume with a plethysmometer. It was found that potassium
humate did not have an
anti-
inflammatory effect on the delayed type
hypersensitivity reaction as opposed to the inhibition caused by
dexamethasone at a dose of 30 mg/kg bodyweight. This study showed
that potassium humate selectively inhibited the
inflammatory
pathway of the carrageenan-induced paw oedema as opposed to the
delayed type hypersensitivity. The mechanism of the
anti-
inflammatory property of potassium humate might possibly be
due to the inhibition of the complement cascade. This study clearly
shows that potassium humate possesses
anti-
inflammatory properties
that can be utilised in the future as a potential treatment for
inflammatory disorders associated with the activation of
complement.…
Advisors/Committee Members: Medlen, C.E (advisor).
Subjects/Keywords: Humate;
Anti-inflammatory properties;
Inflammation;
UCTD
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
author], [. (2008). An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05122008-125709/
Chicago Manual of Style (16th Edition):
author], [No. “An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
.” 2008. Masters Thesis, University of Pretoria. Accessed January 19, 2021.
http://upetd.up.ac.za/thesis/available/etd-05122008-125709/.
MLA Handbook (7th Edition):
author], [No. “An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
.” 2008. Web. 19 Jan 2021.
Vancouver:
author] [. An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Jan 19].
Available from: http://upetd.up.ac.za/thesis/available/etd-05122008-125709/.
Council of Science Editors:
author] [. An evaluation of the anti-inflammatory properties of a
brown coal derived potassium humate
. [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-05122008-125709/
Technical University of Lisbon
28.
Garrido, Madalena Frias do Vale.
Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz.
Degree: 2015, Technical University of Lisbon
URL: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10956
► Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
The present work aims to assess the impact of rice bran ingestion, one of the by-products…
(more)
▼ Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia
The present work aims to assess the impact of rice bran ingestion, one of the by-products still undervalued by Rice Industry on preventing diseases, in particular to evaluate the in vivo administration of two rice bran stabilized and defatted doses. Thus, rodent animal testing/experiments to evaluate possible initial signs of toxicity were made, as well as the evaluation of the effect of ingestion on cholesterol levels and the antioxidant and anti-inflammatory effect of ingested rice bran. These studies revealed that a single administration of bran is not associated with macroscopic or microscopic toxicity signs and it is not possible to establish a effect relation with plasmatic cholesterol levels. However, the repeated ingestion of rice bran may be associated with changes in plasmatic cholesterol levels but it needs further confirmation studies. Nevertheless it was possible to observe that defatted rice bran shows toxicity signs. The study of the antioxidant effect of rice bran did not present relevant conclusions but it was possible to observe that the rice bran ingestion has no beneficial effect on protecting the stress induced by ethanol. As for anti-inflammatory activity for lower doses of the two types of bran tested, it was observed inhibition of the edema although not statistically different from higher doses
Advisors/Committee Members: Corvo, Maria Luísa, Raymundo, Anabela Moreira.
Subjects/Keywords: rice bran; toxicity; cholesterol; antioxidant; anti-inflammatory
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Garrido, M. F. d. V. (2015). Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10956
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Garrido, Madalena Frias do Vale. “Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz.” 2015. Thesis, Technical University of Lisbon. Accessed January 19, 2021.
https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10956.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Garrido, Madalena Frias do Vale. “Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz.” 2015. Web. 19 Jan 2021.
Vancouver:
Garrido MFdV. Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz. [Internet] [Thesis]. Technical University of Lisbon; 2015. [cited 2021 Jan 19].
Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10956.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Garrido MFdV. Avaliação in vivo do potencial de redução de colesterol, da capacidade antioxidante e anti-inflamatória de farelo de arroz. [Thesis]. Technical University of Lisbon; 2015. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10956
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
29.
Naude, P.J.W. (Petrus Johan
Wichardt).
An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate.
Degree: Pharmacology, 2008, University of Pretoria
URL: http://hdl.handle.net/2263/24574
► Humin substances have been used as folk remedies for the last 3000 years. Recent studies have shown that humates possess anti-inflammatory properties, but the mechanism…
(more)
▼ Humin substances have been used as folk remedies for the
last 3000 years. Recent studies have shown that humates possess
anti-
inflammatory properties, but the mechanism of how it affects
inflammation is still unclear. In this study the
anti-
inflammatory
properties of potassium humate, a water soluble humic acid salt,
was investigated on different
inflammatory pathways in vitro and in
vivo. The effect of potassium humate on human mononuclear
lymphocyte proliferation showed that potassium humate stimulated
lymphocyte proliferation of resting-, PHA- and PWM-stimulated
lymphocytes in vitro from concentrations of 20 to 80 µg/ml, in a
dose dependant manner, where a maximum proliferation was observed
at 80 µg/ml whereas lymphocyte proliferation decreased at 100
µg/ml. On the contrary potassium humate, at 40 µg/ml, significantly
inhibited the supernatant concentrations of the following
cytokines; TNF-α, IL-1ß, IL-6 and IL-10 by PHA stimulated
lymphocytes. The effect of potassium humate on the alternative as
well as the classical complement
pathway was investigated in vitro
using the haemolytic complement assay. Results indicated that
potassium humate inhibits both the alternative and classical
complement pathways without affecting the red blood cell membrane
stability. Different
inflammatory mechanisms were investigated in
vivo, using the carrageenan-induced paw oedema model and the
delayed type hypersensitivity reaction model. The
carrageenan-induced paw oedema model was used to determine the
effect of potassium humate on acute inflammation in the hind paw.
Carrageenan was injected into the right hind footpad of a rat which
caused an increase in paw volume due to oedema, which was measured
with a plethysmometer. Potassium humate significantly inhibited the
oedema at a dose of 60 mg/kg bodyweight and compared favourably
with indomethacin at 10 mg/kg bodyweight. The effect of potassium
humate on the delayed type hypersensitivity reaction model was also
investigated whereby rats were sensitised with sheep erythrocytes.
Potassium humate was administered daily by oral gavage at a dose of
60 mg/kg bodyweight. After 7 days, rats were challenged by
injecting sheep erythrocytes into the right hind footpad. The
degree of inflammation was determined by measuring the increase of
paw volume with a plethysmometer. It was found that potassium
humate did not have an
anti-
inflammatory effect on the delayed type
hypersensitivity reaction as opposed to the inhibition caused by
dexamethasone at a dose of 30 mg/kg bodyweight. This study showed
that potassium humate selectively inhibited the
inflammatory
pathway of the carrageenan-induced paw oedema as opposed to the
delayed type hypersensitivity. The mechanism of the
anti-
inflammatory property of potassium humate might possibly be
due to the inhibition of the complement cascade. This study clearly
shows that potassium humate possesses
anti-
inflammatory properties
that can be utilised in the future as a potential treatment for
inflammatory disorders associated with the activation of
complement.…
Advisors/Committee Members: Medlen, C.E. (advisor).
Subjects/Keywords: Humate;
Anti-inflammatory properties;
Inflammation;
UCTD
Record Details
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Share »
Record Details
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Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Naude, P. J. W. (. J. (2008). An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24574
Chicago Manual of Style (16th Edition):
Naude, P J W (Petrus Johan. “An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate.” 2008. Masters Thesis, University of Pretoria. Accessed January 19, 2021.
http://hdl.handle.net/2263/24574.
MLA Handbook (7th Edition):
Naude, P J W (Petrus Johan. “An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate.” 2008. Web. 19 Jan 2021.
Vancouver:
Naude PJW(J. An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/2263/24574.
Council of Science Editors:
Naude PJW(J. An evaluation of
the anti-inflammatory properties of a brown coal derived potassium
humate. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/24574
Oregon State University
30.
Sartnurak, Soisurin.
Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets.
Degree: PhD, Pharmacy, 1985, Oregon State University
URL: http://hdl.handle.net/1957/40873
► Dissolution profiles of two commercial products (Motrine and Rufen®) were analyzed and compared at 8 pH levels, ranging from pH 2.0 to pH 8.0. It…
(more)
▼ Dissolution profiles of two commercial products (Motrine and Rufen®) were analyzed and compared at 8 pH levels, ranging from pH 2.0 to pH 8.0. It was demonstrated, as expected, that the rate and extent of ibuprofen dissolution dissolution was pH dependent. In vitro dissolution characteristics of the ibuprofen solid dispersion formulations prepared by freeze-drying method with various proportions of excipients (theobroma oil, lecithin and PEG 20,000) were investigated at 3 pH levels - pH 2.0, 5.4, and 7.2. As the amount of theobroma oil increased from zero to 31%, the dissolution rate and the percent ibuprofen dissolved was decreased. Freeze-dried systems with a combination of lecithin and PEG 20,000 showed a slower dissolution rate and less amount of drug dissolved than the formulation with only PEG 20,000. The optimal ratio of drug to PEG 20,000 was 1:1. Solid dispersions of ibuprofen prepared by the freezedrying method provided the highest dissolution rate and percentage of drug dissolved when compared with the direct melting method, the solvent method or the physical-mixing method. Dissolution characteristics of the ibuprofen freeze-dried formulation (ratio of drug to PEG 20,000 1:1) were unaffected after storage in 98% relative humidity, but commercial formulation dissolution was drastically reduced. Relative bioavailability of ibuprofen solid dispersed tablets were studied in rabbits after a single oral administration of 50 mg ibuprofen preparations. The freeze-dried solid dispersion formulation with ratio of drug to PEG 1:1 exhibited the greatest relative extent of absorption (129.50 +̲ 27.99% over control). Preparations with PEG 20,000 enhanced the extent of absorption when compared to the formulation of ibuprofen drug powder. There appeared no advantage in formulating ibuprofen in PEG by freeze-drying over the direct melting method. A slower rate of absorption of ibuprofen was obtained when the amount of theobroma oil was increased in the formulation.
Advisors/Committee Members: Christensen, John M. (advisor).
Subjects/Keywords: Anti-inflammatory agents
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sartnurak, S. (1985). Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/40873
Chicago Manual of Style (16th Edition):
Sartnurak, Soisurin. “Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets.” 1985. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021.
http://hdl.handle.net/1957/40873.
MLA Handbook (7th Edition):
Sartnurak, Soisurin. “Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets.” 1985. Web. 19 Jan 2021.
Vancouver:
Sartnurak S. Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets. [Internet] [Doctoral dissertation]. Oregon State University; 1985. [cited 2021 Jan 19].
Available from: http://hdl.handle.net/1957/40873.
Council of Science Editors:
Sartnurak S. Evaluation of solid dispersed particles for formulation of oral ibuprofen tablets. [Doctoral Dissertation]. Oregon State University; 1985. Available from: http://hdl.handle.net/1957/40873
◁ [1] [2] [3] [4] [5] … [371] ▶
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Open Access Theses and Dissertations
