You searched for subject:(cell printing).
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University of Wollongong
1.
Ferris, Cameron.
Bio-inks for drop-on-demand cell printing.
Degree: PhD, 2013, University of Wollongong
URL: 090301
Biomaterials,
090304
Medical
Devices,
100404
Regenerative
Medicine
(incl.
Stem
Cells
and
Tissue
Engineering)
;
https://ro.uow.edu.au/theses/3875 
► A rapidly growing synergy between biological science and engineering technology is currently re-shaping the way we view the challenge of treating injury and disease.…
(more)
▼ A rapidly growing synergy between biological science and engineering technology is currently re-shaping the way we view the challenge of treating injury and disease. In particular, emerging biofabrication techniques that allow the precise construction of complex biological structures have reinvigorated the effort to engineer replacement tissues and organs. In addition to the potential for direct therapeutic approaches, advanced engineered tissues promise to significantly improve in vitro studies of fundamental cell biology and disease processes, and expedite drug development.
Drop-on-demand cell printing technologies are at the forefront of these advances in biofabrication. These approaches offer the ability to place living cells, biomaterials and other factors in defined arrangements in two or three dimensions in order to reproduce the complex spatial interplay that regulates tissue function. Significant progress towards this goal has been made over the last decade, but the design of bioinks remains a key challenge due to the need to simultaneously satisfy disparate engineering and biological requirements. The aim of this thesis was to develop bioinks for drop-on-demand cell printing that enable the robust deposition of living cells. Specifically, a suitable bio-ink should be non-cytotoxic, prevent cell settling and aggregation, possess optimal fluid properties (i.e. viscosity and surface tension) for drop-on-demand printing and contain minimal dry mass.
Bio-inks were developed by forming gellan gum (GG) microgel suspensions in cell culture media by applying shear during gelation. At a low polymer concentration (0.05% w/v) the bio-ink showed a yield stress (~ 43 mPa), while exhibiting a low viscosity (~ 1.7 mPa.s) at high shear rates (103 s-1). These properties were shown to prevent cell settling and aggregation without affecting printability. Surfactants were added to the formulation to achieve surface tension reduction for inkjet printing. Addition of the fluorosurfactant, Novec FC-4430, allowed a suitable surface tension (~ 30 mN/m at 0.05% v/v) to be achieved, while Poloxamer 188 (P188) was included (0.1% v/v) for its reported cell-protecting qualities. Neither surfactant significantly affected the bio-ink structure or rheology, and C2C12 (skeletal muscle) and PC12 (pheocromocytoma) cells exposed to the surfactant-containing bio-ink for 2 hr exhibited normal viability, proliferation and differentiation.
The bio-ink formulations, with and without surfactants, proved suitable for cell deposition by microvalve and inkjet printing, respectively. The bio-ink enabled reproducible cell output over 1 hr printing periods from both a microvalve printer (Deerac Equator GX1) and multiple-nozzle piezoelectric inkjet print heads (Xaar-126). Printed cells exhibited phenotypic responses that were comparable to controls. It was also demonstrated that P188 had a protective effect on cells during inkjet printing.
Inkjet cell printing using the bio-ink was applied to the fabrication of two dimensional…
Subjects/Keywords: IPRI; Biofabrication; cell printing; hydrogel; tisue engineering
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APA (6th Edition):
Ferris, C. (2013). Bio-inks for drop-on-demand cell printing. (Doctoral Dissertation). University of Wollongong. Retrieved from 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875
Chicago Manual of Style (16th Edition):
Ferris, Cameron. “Bio-inks for drop-on-demand cell printing.” 2013. Doctoral Dissertation, University of Wollongong. Accessed April 14, 2021.
090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875.
MLA Handbook (7th Edition):
Ferris, Cameron. “Bio-inks for drop-on-demand cell printing.” 2013. Web. 14 Apr 2021.
Vancouver:
Ferris C. Bio-inks for drop-on-demand cell printing. [Internet] [Doctoral dissertation]. University of Wollongong; 2013. [cited 2021 Apr 14].
Available from: 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875.
Council of Science Editors:
Ferris C. Bio-inks for drop-on-demand cell printing. [Doctoral Dissertation]. University of Wollongong; 2013. Available from: 090301 Biomaterials, 090304 Medical Devices, 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) ; https://ro.uow.edu.au/theses/3875
Virginia Tech
2.
Jiang, Yuanyuan.
Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray.
Degree: PhD, Mechanical Engineering, 2019, Virginia Tech
URL: http://hdl.handle.net/10919/101052
► Perovskite solar cells have attracted much attention both in research and industrial domains. An unprecedented progress in development of hybrid perovskite solar cells (HPSCs) has…
(more)
▼ Perovskite solar cells have attracted much attention both in research and industrial domains. An unprecedented progress in development of hybrid perovskite solar cells (HPSCs) has been seen in past few years. The power conversion efficiencies of HPSCs has been improved from 3.8% to 24.2% in less than a decade, rivaling that of silicon solar cells which currently dominate the solar
cell market. Hybrid perovskite materials have exceptional opto-electrical properties and can be processed using cost-effective solution-based methods. In contrast, fabrication of silicon solar cells requires high-vacuum, high-temperature, and energy intensive processes. The combination of excellent opto-electrical properties and cost-effective manufacturing makes hybrid perovskite a winning candidate for solar cells.
As power conversion efficiencies of HPSCs improves beyond that of the established solar
cell technology and their long-term stability increases, one of the crucial hurdles in the path to commercialization remaining to be adequately addressed is the cost-effective scalable fabrication. Spin-coating is the prevailing method for fabrication of HPSCs in laboratories. However, this technique is limited to small areas and results in excessive material waste. Two types of scalable manufacturing methods have been successfully demonstrated to fabricate HPSCs: (i) meniscus-assisted coating such as doctor-blade coating and slot-die coating; and (ii) dispersed deposition based on the coalescence of individual droplets, such as inkjet
printing and spray coating. Electrospray
printing belongs to the second category with advantages of high material utilization rate and patterning capability along with the scalability and roll-to-roll compatibility.
In Chapter 3 of this dissertation, electrospray
printing process is described for manufacturing of HPSCs in ambient conditions below 150 C. All three functional layers were printed using electrospray
printing including perovskite layer, electron transport layer, and hole transport layer. Strategies for successful electrospray
printing of HPSCs include formulation of the precursor inks with solvents of low vapor pressures, judicial choice of droplet flight time, and tailoring the wetting property of the substrate to suppress coffee ring effects. Implementation of these strategies leads to pin-hole free, low surface roughness, and uniform perovskite layer, hole transport layer and electron transport layer. The power conversion efficiency of the all electrospray printed device reached up to 15.0%, which is among the highest to date for fully printed HPSCs.
The most efficient HPSCs rely on gold and organic hole-transport materials (HTMs) for achieving high performance. Gold is also chosen for its high stability. Unfortunately, the high price of gold and high-vacuum along with high-temperature processing requirements for gold film is not suitable for the large-scale fabrication of HPSCs. Carbon is a cheap alternative electrode material which is inert to hybrid perovskite layer. Due to the…
Advisors/Committee Members: Priya, Shashank (committeechair), Deng, Weiwei (committeechair), Cheng, Jiangtao (committee member), Chen, Cheng (committee member), Li, Zheng (committee member).
Subjects/Keywords: Perovskite Solar Cell; Electrospray Printing; Scalable Preparation
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APA (6th Edition):
Jiang, Y. (2019). Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/101052
Chicago Manual of Style (16th Edition):
Jiang, Yuanyuan. “Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray.” 2019. Doctoral Dissertation, Virginia Tech. Accessed April 14, 2021.
http://hdl.handle.net/10919/101052.
MLA Handbook (7th Edition):
Jiang, Yuanyuan. “Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray.” 2019. Web. 14 Apr 2021.
Vancouver:
Jiang Y. Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray. [Internet] [Doctoral dissertation]. Virginia Tech; 2019. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10919/101052.
Council of Science Editors:
Jiang Y. Scalable Fabrication of High Efficiency Hybrid Perovskite Solar Cells by Electrospray. [Doctoral Dissertation]. Virginia Tech; 2019. Available from: http://hdl.handle.net/10919/101052
University of New South Wales
3.
Tan, Vincent Tit Guan.
Synthesis of Polymeric Hydrogel for 3D Cell Culturing.
Degree: Chemistry, 2018, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/60223
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51255/SOURCE02?view=true
► Over the past decade there has been high interest in understanding cell behaviour in an in vivo setting and with that hydrogel have been drawn…
(more)
▼ Over the past decade there has been high interest in understanding
cell behaviour in an in vivo setting and with that hydrogel have been drawn into light. Hydrogels have been used in a wide scope of applications such as tissue engineering and stem
cell differentiation being the key examples.1, 2 However, the main application which peaked scientist interest is their functionality as an extracellular matrix (ECM) mimic.3 It has been widely accepted that
cell behaviour (proliferation, migration, invasion) is influenced by the surrounding environment. That is, a 2 dimensional environment provides different biological cues to a
cell compared to a 3 dimensional environment. One of the main factors which come into play is mechanotransduction; the influence of mechanical forces on a
cell which translate into electrochemical stimuli.4 Understanding
cell response to different environments will be critical in developing a
cell culturing platform that resembles the in vivo settings. This thesis outlines 3 bodies of work; each contributing to the development of the next hydrogel. The first hydrogel is based on traditional formation whereby a stimulus (light) is used to cause gelation. Understanding the promise and limitation of the light based hydrogel a borax based hydrogel was developed. The premise of the borax hydrogel was to use commercially available chemicals to form the ECM mimics with options to change various components to introduce other biological stimulus i.e. peptide crosslinkers. Finally, a maleimide based hydrogel was developed in conjunction with a 3D printer and commercially available chemicals. Automation and pre-made chemicals remove inconsistencies and provide commercial advantage. This is the first report of an “off the shelf” ECM mimic which can be printed and manipulated specifically for various
cell types.
Advisors/Committee Members: Gooding, J. Justin, Chemistry, Faculty of Science, UNSW.
Subjects/Keywords: Polymer; Hydrogel; 3D printing; Cell culturing
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APA (6th Edition):
Tan, V. T. G. (2018). Synthesis of Polymeric Hydrogel for 3D Cell Culturing. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/60223 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51255/SOURCE02?view=true
Chicago Manual of Style (16th Edition):
Tan, Vincent Tit Guan. “Synthesis of Polymeric Hydrogel for 3D Cell Culturing.” 2018. Doctoral Dissertation, University of New South Wales. Accessed April 14, 2021.
http://handle.unsw.edu.au/1959.4/60223 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51255/SOURCE02?view=true.
MLA Handbook (7th Edition):
Tan, Vincent Tit Guan. “Synthesis of Polymeric Hydrogel for 3D Cell Culturing.” 2018. Web. 14 Apr 2021.
Vancouver:
Tan VTG. Synthesis of Polymeric Hydrogel for 3D Cell Culturing. [Internet] [Doctoral dissertation]. University of New South Wales; 2018. [cited 2021 Apr 14].
Available from: http://handle.unsw.edu.au/1959.4/60223 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51255/SOURCE02?view=true.
Council of Science Editors:
Tan VTG. Synthesis of Polymeric Hydrogel for 3D Cell Culturing. [Doctoral Dissertation]. University of New South Wales; 2018. Available from: http://handle.unsw.edu.au/1959.4/60223 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:51255/SOURCE02?view=true
Penn State University
4.
Guo, Feng.
Acoustic tweezers: manipulating micro-objects with the power of sound
.
Degree: 2015, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/27341
► Sound can be music to please the ear, however the waves produced can be utilized as “Acoustic Tweezers” for the manipulation of cells and particles…
(more)
▼ Sound can be music to please the ear, however the waves produced can be utilized as “Acoustic Tweezers” for the manipulation of cells and particles in a fluid medium.
The ability to dexterously and noninvasively manipulate biological specimens such as organisms, cells, proteins, and DNAs in a compact system is critical for many applications in the fields of life sciences, biomedicine and chemistry. Acoustic tweezer technology is a revolutionary way to satisfy this requirement. Firstly, this technique manipulates cells or particles using gentle mechanical vibrations. These vibrations create a pressure gradient in the medium to move suspended micro-objects yielding a contamination-free, non-contact, and label-free manipulation. Secondly, acoustic tweezers have minimal impact on
cell viability and function, which operates at a power intensity and frequency similar to the widely used medical ultrasound imaging. Thirdly, the acoustic tweezer device can operate in a single micro-device without any external moving parts or complicate setups, which offer additional advantages in ease of use, versatility and portability.
In this dissertation, we have developed a series of acoustic tweezers that can achieve manipulation of micro-objects in a liquid medium: 1) tunable acoustic wells to control
cell-cell distance and geometry of suspended
cell assemblies; 2) 3D acoustic tweezers to dexterously transport single cells in a three-dimensional manner; 3) simple, low-cost and reusable acoustic tweezers used for various disposable devices; and 4) application of the reusable acoustic tweezer technology in precisely manipulating and patterning micrometer-sized protein crystals for X-ray crystallography.
Advisors/Committee Members: Jun Huang, Dissertation Advisor/Co-Advisor, Jun Huang, Committee Chair/Co-Chair, Bernhard R Tittmann, Committee Member, Bruce Gluckman, Committee Member, Corina Stefania Drapaca, Committee Member, Siyang Zheng, Special Member.
Subjects/Keywords: acoustic tweezers; microfluidics; cell-cell interaction; cell printing; crystallography; disposable device.
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APA (6th Edition):
Guo, F. (2015). Acoustic tweezers: manipulating micro-objects with the power of sound
. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27341
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Guo, Feng. “Acoustic tweezers: manipulating micro-objects with the power of sound
.” 2015. Thesis, Penn State University. Accessed April 14, 2021.
https://submit-etda.libraries.psu.edu/catalog/27341.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Guo, Feng. “Acoustic tweezers: manipulating micro-objects with the power of sound
.” 2015. Web. 14 Apr 2021.
Vancouver:
Guo F. Acoustic tweezers: manipulating micro-objects with the power of sound
. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Apr 14].
Available from: https://submit-etda.libraries.psu.edu/catalog/27341.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Guo F. Acoustic tweezers: manipulating micro-objects with the power of sound
. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27341
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Rochester Institute of Technology
5.
Fan, Rong.
Cell Dynamics in Three-dimensional (3D) Culture Environments.
Degree: PhD, Microsystems Engineering, 2017, Rochester Institute of Technology
URL: https://scholarworks.rit.edu/theses/9507
► A three-dimensional (3D) cell culture system provides an effective platform to study cell dynamics in in vivo-mimicking conditions and thus plays an important role…
(more)
▼ A three-dimensional (3D)
cell culture system provides an effective platform to study
cell dynamics in in vivo-mimicking conditions and thus plays an important role in understanding
cell biology, organ function, and disease model. This dissertation investigates
cell dynamics in a variety of 3D environments including solid and liquid matrix. We study
cell dynamics in 3D hydrogel microparticles and show that cells exhibit significant differences with that from 2D monolayer culture, including
cell cycle, survival, morphology and the sensitivity to inflammation. We further develop a 3D printed
cell-laden hybrid hydrogel construct to investigate colon cancer
cell dynamics in physiologically relevant bowel environment. Such system enables in vivo-mimicking
cell environment and offers an effective platform to uncover inflammation mechanisms in bowel area. Long-term
cell culture in 3D solid matrix, however, is challenged by nutrient delivering problems. We thus engineer a novel leaf-inspired artificial microvascular network to support the long-term
cell growth. Apart from the 3D solid environment, we also investigate
cell dynamics cultured in 3D fluidic environment and study the regulatory roles of shear stress in circulating cancer cells. Cancer cells are circulated in suspension for mimicking cancer metastasis through blood stream and a previously unrecognized role of circulatory shear stress in regulating cancer
cell dynamics is revealed. The research presented in this dissertation introduces a comprehensive study of
cell dynamics in 3D environments and paves a new avenue to establish physiologically relevant model systems for tissue engineering and artificial functional organs.
Advisors/Committee Members: Jiandi Wan.
Subjects/Keywords: 3D cell culture; 3D printing; Cell biology; Microfluidics; Vascular system
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APA (6th Edition):
Fan, R. (2017). Cell Dynamics in Three-dimensional (3D) Culture Environments. (Doctoral Dissertation). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/9507
Chicago Manual of Style (16th Edition):
Fan, Rong. “Cell Dynamics in Three-dimensional (3D) Culture Environments.” 2017. Doctoral Dissertation, Rochester Institute of Technology. Accessed April 14, 2021.
https://scholarworks.rit.edu/theses/9507.
MLA Handbook (7th Edition):
Fan, Rong. “Cell Dynamics in Three-dimensional (3D) Culture Environments.” 2017. Web. 14 Apr 2021.
Vancouver:
Fan R. Cell Dynamics in Three-dimensional (3D) Culture Environments. [Internet] [Doctoral dissertation]. Rochester Institute of Technology; 2017. [cited 2021 Apr 14].
Available from: https://scholarworks.rit.edu/theses/9507.
Council of Science Editors:
Fan R. Cell Dynamics in Three-dimensional (3D) Culture Environments. [Doctoral Dissertation]. Rochester Institute of Technology; 2017. Available from: https://scholarworks.rit.edu/theses/9507
Penn State University
6.
Li, Harvey S.
THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS.
Degree: 2018, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/15391hsl5047
► When using the correct material coupled with drugs and specific surface ligands, nanoparticles can have enhanced targeted drug delivery properties. Currently, scientists can alter the…
(more)
▼ When using the correct material coupled with drugs and specific surface ligands, nanoparticles can have enhanced targeted drug delivery properties. Currently, scientists can alter the size, shape, material, and surface properties among other factors in order to change the characteristics of the nanoparticles and how the body reacts to them1. While much research has been done looking at how varying the properties of nanoparticles affect nanoparticle uptake, little research has been done to see how varying
cell properties affect nanoparticle uptake. Additionally, it is known that the extracellular matrix of tumor tissue is different than that of healthy tissue. The differences in ECM organization causes the tumor cells to take on certain patterns, which may affect nanoparticle and drug uptake. The aims of this project are twofold: first, I will compare the rate of nanoparticle uptake at the leading edge to that at the trailing edge, then, I would like to see how
cell morphology affects nanoparticle uptake. The results from these experiments indicate that there is a difference in nanoparticle uptake in elongated and non-elongated cells. The leading edge of a
cell also appears to contain more nanoparticles than the trailing edge of a
cell; however, this is not dependent on cellular elongation.
Advisors/Committee Members: Justin Lee Brown, Thesis Advisor/Co-Advisor, William O Hancock, Committee Member, Xiaojun Lian, Committee Member.
Subjects/Keywords: cell; cell morphology; nanoparticles; endocytosis; immunostaining; live-cell imaging; MC3T3 cells; microcontact printing; y-27632
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APA (6th Edition):
Li, H. S. (2018). THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/15391hsl5047
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, Harvey S. “THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS.” 2018. Thesis, Penn State University. Accessed April 14, 2021.
https://submit-etda.libraries.psu.edu/catalog/15391hsl5047.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, Harvey S. “THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS.” 2018. Web. 14 Apr 2021.
Vancouver:
Li HS. THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS. [Internet] [Thesis]. Penn State University; 2018. [cited 2021 Apr 14].
Available from: https://submit-etda.libraries.psu.edu/catalog/15391hsl5047.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li HS. THE EFFECTS OF CELL MORPHOLOGY ON NANOPARTICLE UPTAKE AND UPTAKE AT THE LEADING EDGE IN MC3T3 CELLS. [Thesis]. Penn State University; 2018. Available from: https://submit-etda.libraries.psu.edu/catalog/15391hsl5047
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Clemson University
7.
Gudapati, Hemanth.
STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION.
Degree: MS, Mechanical Engineering, 2012, Clemson University
URL: https://tigerprints.clemson.edu/all_theses/1560
► Laser-assisted cell printing, developed based on Matrix-assisted pulsed-laser evaporation direct-write (MAPLE DW), a typical LIFT (laser-induced forward transfer) practice, has been emerging as one…
(more)
▼ Laser-assisted
cell printing, developed based on Matrix-assisted pulsed-laser evaporation direct-write (MAPLE DW), a typical LIFT (laser-induced forward transfer) practice, has been emerging as one of the most promising biofabrication techniques. Alginate, particularly sodium alginate, is extensively used as the constituent of bioink in laser-assisted
cell printing. However, thus far, studies investigating the effect of alginate gelation on
cell viability in laser-assisted
cell printing are lacking. The objective of this study is to investigate the effects of gelation, gelation time, sodium alginate concentration, and the effect of operating conditions such as the laser fluence on post-transfer
cell viability during laser-assisted
cell printing. Two experimental setups have been designed in this study. Experimental setup A was characterized by laser fluences 800, 1200, and 1600 mJ/cm2 and a constant alginate concentration of 1% w/v with 5 _ 106 NIH3T3 cells/ml in bioink. Experimental setup B was characterized by alginate concentrations of 1, 2, and 3 % w/v with 5 _ 106 NIH3T3 cells/ml in bioink and a constant laser fluence of 800 mJ/cm2. Experimental setup A was designed to study the effects of gelation, gelation time, and laser fluence on post-transfer
cell viability. Experimental setup B was designed to study effects of gelation and sodium alginate concentration on post-transfer
cell viability. Furthermore,
cell-laden alginate droplets were subjected to no gelation, two-minute gelation, or ten-minute gelation.
Cell viability was evaluated immediately after
printing and after 24 hours of incubation. Process-induced
cell injury during alginate gelation in laser-assisted
cell printing is systematically elucidated through investigating the effects of operating conditions and material properties on the post-transfer
cell viability and
cell injury reversibility. Two-minute gelation is observed to increase
cell viability over 24 hours because of cushion effect. That is, forming gel membrane has minimized the impact of mechanical stresses generated during droplet landing. Despite ten minutes gelation having a cushion effect during droplet landing, it is observed to decrease
cell viability over 24 hours because of the thick gel membrane which reduces nutrient diffusion from culture medium. Also, the longer exposure of encapsulated cells to calcium chloride has resulted in greater
cell injury due to Ca2+ ions. Increase in laser fluence as well as alginate concentration is observed to decrease
cell viability by introducing greater mechanical stresses during droplet formation. The process-induced
cell death is modeled using power-law and Gompertz models. Gompertz model is observed to better predict
cell viability than power-law model. However, the two models ignore molecular signaling pathways that govern the
cell responses. Hence, future studies have to model process-induced
cell death based on molecular signaling pathways.
Advisors/Committee Members: Huang, Yong, Nagatomi , Jiro, Figliola , Richard.
Subjects/Keywords: Alginate; Cell injury; Cell viability; Gelation; laser cell printing; Microspheres; Mechanical Engineering
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APA (6th Edition):
Gudapati, H. (2012). STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/1560
Chicago Manual of Style (16th Edition):
Gudapati, Hemanth. “STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION.” 2012. Masters Thesis, Clemson University. Accessed April 14, 2021.
https://tigerprints.clemson.edu/all_theses/1560.
MLA Handbook (7th Edition):
Gudapati, Hemanth. “STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION.” 2012. Web. 14 Apr 2021.
Vancouver:
Gudapati H. STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION. [Internet] [Masters thesis]. Clemson University; 2012. [cited 2021 Apr 14].
Available from: https://tigerprints.clemson.edu/all_theses/1560.
Council of Science Editors:
Gudapati H. STUDY OF CELL VIABILITY DURING LASER DIRECT WRITING OF CELL-ALGINATE SUSPENSION. [Masters Thesis]. Clemson University; 2012. Available from: https://tigerprints.clemson.edu/all_theses/1560
8.
Dzhoyashvili, Nina.
Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
.
Degree: 2017, National University of Ireland – Galway
URL: http://hdl.handle.net/10379/6676
► One promising direction of regenerative medicine is the development of cell sheet-based tissue-like constructs. The cell sheets preserve ECM and cell−cell junctions. This may greatly…
(more)
▼ One promising direction of regenerative medicine is the development of
cell sheet-based tissue-like constructs. The
cell sheets preserve ECM and cell−
cell junctions. This may greatly support
cell adherence to damaged organ after transplantation. Furthermore, the
cell sheets might be used as a building block to engineer large biological tissues with complex organizational architecture. This could be achieved by integrating
cell sheets with three-dimensional biomaterial scaffolds.
In this study, poly (N-isopropylacrylamide) films were used to produce
cell sheets. The pNIPAm films were fabricated by a spin-coating technique. The spin-coating technique allows rapid fabrication of pNIPAm substrates with high reproducibility and uniformity.
Because the method of polymer deposition can significantly impact the biological properties of pNIPAm films, the dynamics of
cell behavior on spin-coated pNIPAm films of different thicknesses were first examined. Next, biological properties of harvested stromal and epithelial
cell sheets after manipulation such as detachment from pNIPAm films, transfer, and re-attachment were assessed. The
cell morphology, the pattern and speed of
cell sheet recovery and total
cell number in
cell sheets were analyzed. In addition, the metabolic activity and
cell viability of
cell sheets before and after detachment and re-attachment were also examined. Next, an integrated-design approach was used to create three-dimensional constructs from
cell sheets and three-dimensional natural (acellular pericardial matrix) or three-dimensional synthetic (two-photon polymerization-generated or surface selective laser sintered) scaffolds.
These findings should promote further development of implantable tissues engineered from tissue-specific
cell sheets and three-dimensional scaffolds.
Advisors/Committee Members: Rochev, Yury (advisor).
Subjects/Keywords: pNIPAm films, cell sheet engineering, 3D printing, tissue decellularization;
Chemistry;
pNIPAm films;
Cell sheet engineering;
3D printing;
Tissue decellularization
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APA (6th Edition):
Dzhoyashvili, N. (2017). Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
. (Thesis). National University of Ireland – Galway. Retrieved from http://hdl.handle.net/10379/6676
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dzhoyashvili, Nina. “Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
.” 2017. Thesis, National University of Ireland – Galway. Accessed April 14, 2021.
http://hdl.handle.net/10379/6676.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dzhoyashvili, Nina. “Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
.” 2017. Web. 14 Apr 2021.
Vancouver:
Dzhoyashvili N. Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
. [Internet] [Thesis]. National University of Ireland – Galway; 2017. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10379/6676.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dzhoyashvili N. Integrating the old and new: smart thermoresponsive surfaces and 3D fabrication technologies for tissue engineering
. [Thesis]. National University of Ireland – Galway; 2017. Available from: http://hdl.handle.net/10379/6676
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Penn State University
9.
O'connor, Joseph William.
Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition.
Degree: 2015, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/27307
► Myofibroblasts are specialized cells that exert large contractile forces to assist in the closure of wounds. Aberrant and chronic activation of myofibroblasts can contribute to…
(more)
▼ Myofibroblasts are specialized cells that exert large contractile forces to assist in the closure of wounds. Aberrant and chronic activation of myofibroblasts can contribute to the development of pathological conditions including cancer, fibrosis, and the foreign-body response to implanted biomaterials. Myofibroblasts can develop from epithelial cells through an epithelial-mesenchymal transition (EMT) which can be mediated by a combination of the chemical signal transforming growth factor (TGF)-beta1 and mechanical stimuli. During EMT, epithelial cells detach from adjacent cells and acquire an elongated, mesenchymal-like morphology. These phenotypic changes are accompanied by changes in the expression of epithelial and mesenchymal markers including up-regulation of a variety of cytoskeletal associated proteins such as alpha smooth muscle actin (alphaSMA). A better understanding of the mechanistic underpinnings of how
cell and tissue level physical properties contribute to EMT in pathological settings is needed. In order to accomplish this goal, we employ a wide-array of molecular biology tools and engineered
cell culture platforms. We find that a combination of TGFbeta1 signaling and biophysical cues, such as
cell-matrix adhesion,
cell-cell interactions, and epigenetic remodeling, regulate the development of myofibroblasts from epithelial cells. Furthermore, we identify myocardin related transcription factor (MRTF)-A as a key mechanosensitive regulator in several of these processes. This dissertation provides insight into how the cellular microenvironment can control EMT and may suggest ways to enhance wound healing or to engineer therapeutic and diagnostic solutions for fibrosis and cancer.
Advisors/Committee Members: Esther Winter Gomez, Dissertation Advisor/Co-Advisor, Manish Kumar, Committee Member, Andrew Zydney, Committee Member, Cheng Dong, Committee Member.
Subjects/Keywords: epithelial mesenchymal transition; cytoskeleton; cell adhesion; TGFbeta signaling; epigenetics; microcontact printing
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APA (6th Edition):
O'connor, J. W. (2015). Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
O'connor, Joseph William. “Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition.” 2015. Thesis, Penn State University. Accessed April 14, 2021.
https://submit-etda.libraries.psu.edu/catalog/27307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
O'connor, Joseph William. “Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition.” 2015. Web. 14 Apr 2021.
Vancouver:
O'connor JW. Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Apr 14].
Available from: https://submit-etda.libraries.psu.edu/catalog/27307.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
O'connor JW. Biophysical Regulation of TGFbeta1-induced epithelial mesenchymal transition. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27307
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Tasmania
10.
Cecil, F.
A step towards fully automated 3D printed instruments.
Degree: 2019, University of Tasmania
URL: https://eprints.utas.edu.au/31441/1/Cecil_whole_thesis.pdf
► In the past few decades, significant advancement has been made towards the development of automated microfluidic platforms because of their unique advantages such as integration…
(more)
▼ In the past few decades, significant advancement has been made towards the development of automated microfluidic platforms because of their unique advantages such as integration of different functions, minimum consumption of samples/reagents, low cost, and quick sample analysis. Various components of an instrument e.g. detectors, flow cell, pumps etc., are usually customised in order to improve instrument performance in terms of analysis time, liquid sample and reagents consumption, and reliability through automation. 3D printing, a rapidly growing manufacturing technique, is an attractive alternative to traditional manufacturing techniques (e.g. subtractive manufacturing) because of its ability to print almost any structure on demand with minimal time and cost. The focus of this study was to fabricate and characterise key components of the microfluidic systems including detectors, flow cell, and pumps, and potentially integrate them to produce automated analytical instrument for application in microfluidics.
First, this study explored the potential of a commonly available low cost FDM 3D printer to manufacture a photometric detector body consisting of integrated slit and hollow structures, to position a LED and photodiode, on either side of capillary tubing (Chapter 2). The spatial orientation for printing was investigated to facilitate the printing of a narrow size slit suitable for capillaries and tubing (i.d. 50 μm to 10 mm). A slit of 70 μm was printed when the slit was positioned in the XY plane in parallel with the print direction. The detection body with integrated slit showed satisfactory performance for both large diameter tubing and narrow capillaries. The performance of the 3D printed housing with a 70 μm slit was compared with a commercial CE interface for the CE separation of Zn(2+) and Cu(2+) complexes with PAR.
The second aim of this study was to develop a flow cell with an integrated channel to avoid the need for external capillaries and tubing. Chapter 3 describes the use of multi-material 3D printing technology for the fabrication of a complex photometric detector flow cell with integrated channel and slit. Multi material 3D printing allowed the fabrication of detection window using transparent material, and channel and flow cell body using an opaque material, all printed in one piece. The flow-cell was optimised by varying the design features including slit dimension and optical path-length. The performance of the printed flow cell devices was characterised with a standard dye solution by determining the stray light %, effective path-length and the signal to noise ratio. A device consisting of 500 μm slit with 10 mm optical path-length showed best performance.
Third, the use of multi-material FDM 3D printing to manufacture an electroosmotic pump (EOP) with multiple functionalities was investigated as fluid pumping is a key feature of a microfluidic system and usually requires a micro pump manipulate liquid flow at a smaller scale (≤ 1 mm). Chapter 4 presents the first use of this…
Subjects/Keywords: 3D printing; Fused Deposition Modelling; FDM; detectors; flow cell; electroosmotic pump
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APA (6th Edition):
Cecil, F. (2019). A step towards fully automated 3D printed instruments. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/31441/1/Cecil_whole_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cecil, F. “A step towards fully automated 3D printed instruments.” 2019. Thesis, University of Tasmania. Accessed April 14, 2021.
https://eprints.utas.edu.au/31441/1/Cecil_whole_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cecil, F. “A step towards fully automated 3D printed instruments.” 2019. Web. 14 Apr 2021.
Vancouver:
Cecil F. A step towards fully automated 3D printed instruments. [Internet] [Thesis]. University of Tasmania; 2019. [cited 2021 Apr 14].
Available from: https://eprints.utas.edu.au/31441/1/Cecil_whole_thesis.pdf.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cecil F. A step towards fully automated 3D printed instruments. [Thesis]. University of Tasmania; 2019. Available from: https://eprints.utas.edu.au/31441/1/Cecil_whole_thesis.pdf
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
California State University – San Bernardino
11.
Minck, Justin Stewart.
DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS.
Degree: MSin Biology, Biology, 2019, California State University – San Bernardino
URL: https://scholarworks.lib.csusb.edu/etd/844
► Organ transplantation has made great progress since the first successful kidney transplant in 1953 and now more than one million tissue transplants are performed…
(more)
▼ Organ transplantation has made great progress since the first successful kidney transplant in 1953 and now more than one million tissue transplants are performed in the United States every year (www.organdonor.gov/statistics-stories, 2015). However, the hope and success of organ transplants are often overshadowed by their reputation as being notoriously difficult to procure because of donor-recipient matching and availability. In addition, those that are fortunate enough to receive a transplant are burdened with a lifetime of immunosuppressants. The field of regenerative medicine is currently making exceptional progress toward making it possible for a patient to be their own donor. Cells from a patient can be collected, reprogrammed into stem cells, and then differentiated into specific
cell types. This technology combined with recent advances in 3D
printing provides a unique opportunity. Cells can now be accurately deposited with computerized precision allowing tissue engineering from the inside out (Gill, 2016). However, more work needs to be done as these techniques have yet to be perfected. Bioprinters can cost hundreds of thousands of dollars, and the bioink they consume costs thousands per liter. The resulting cost in development of protocols required for effective tissue
printing can thus be cost-prohibitive, limiting the research to labs which can afford this exorbitant cost and in turn slowing the progress made in the eventual creation of patient derived stem
cell engineered organs.
The objective of my research is to develop a simple and low-cost introductory system for biological additive manufacturing (Otherwise known as 3D bioprinting). To create an easily accessible and cost-effective system several design constraints were implemented. First, the system had to use mechanical components that could be purchased “off-the-shelf” from commonly available retailers. Second, any mechanical components involved had to be easily sterilizable, modifiable, and compatible with open-source software. Third, any customized components had to be fabricated using only 3D
printing and basic tools (i.e. saw, screwdriver, and wrench). Fourth, the system and any expendable materials should be financially available to underfunded school labs, in addition to being sterilizable, biocompatible, customizable, and biodegradable. Finally, all hardware and expendables had to be simple enough as to be operated by high school science students.
Advisors/Committee Members: Bournias-Vardiabasis, Nicole.
Subjects/Keywords: Bioprinting; 3D-Printing; Cell Culture; Bioink; STEM Education; 3T3 Cells; Biotechnology
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APA (6th Edition):
Minck, J. S. (2019). DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS. (Thesis). California State University – San Bernardino. Retrieved from https://scholarworks.lib.csusb.edu/etd/844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Minck, Justin Stewart. “DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS.” 2019. Thesis, California State University – San Bernardino. Accessed April 14, 2021.
https://scholarworks.lib.csusb.edu/etd/844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Minck, Justin Stewart. “DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS.” 2019. Web. 14 Apr 2021.
Vancouver:
Minck JS. DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS. [Internet] [Thesis]. California State University – San Bernardino; 2019. [cited 2021 Apr 14].
Available from: https://scholarworks.lib.csusb.edu/etd/844.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Minck JS. DEVELOPING A LOW COST BIOLOGICAL ADDITIVE MANUFACTURING SYSTEM FOR FABRICATING GEL EMBEDDED CELLULAR CONSTRUCTS. [Thesis]. California State University – San Bernardino; 2019. Available from: https://scholarworks.lib.csusb.edu/etd/844
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Illinois – Chicago
12.
D'Angelo, Lorenzo.
3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production.
Degree: 2016, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21259
► Human civilization faces major challenges regarding sustainable energy production. Biomass, such as agricultural waste, is an abundant source of underutilized chemical energy, and therefore, has…
(more)
▼ Human civilization faces major challenges regarding sustainable energy production. Biomass,
such as agricultural waste, is an abundant source of underutilized chemical energy, and therefore,
has potential to replace or supplement fossil fuel use in the future. Currently, an effective
way to convert biomass to energy is through a microbial fuel
cell (MFC), a type of biofuel
cells utilizing bacteria to convert the chemical energy of biomass directly into electrical energy.
However, current MFCs produce too little power to be useful in practical applications. One
potential solution is to miniaturize and stack many MFCs together for high power production.
In this thesis a new stack design fabricated with the help of an advanced 3D
printing technology
is presented, different electrode materials are tested and the different performances are
characterized. Also an innovative electrode has been designed but without being able to be
fabricated with the standard technology available.
Advisors/Committee Members: Xu, Jie (advisor), Uslenghi, Piergiorgio (committee member), Asinari, Pietro (committee member).
Subjects/Keywords: MFC; Fuel cell; Bacteria; Electrodes; CNT; Stack; Photolitography; 3D printing
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APA (6th Edition):
D'Angelo, L. (2016). 3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21259
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
D'Angelo, Lorenzo. “3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production.” 2016. Thesis, University of Illinois – Chicago. Accessed April 14, 2021.
http://hdl.handle.net/10027/21259.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
D'Angelo, Lorenzo. “3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production.” 2016. Web. 14 Apr 2021.
Vancouver:
D'Angelo L. 3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/10027/21259.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
D'Angelo L. 3D Printed Microfluidic Microbial Fuel Cell Stack for Portable Electricity Production. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/21259
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Bath
13.
Rymansaib, Zuhayr.
Printing materials and processes for electrochemical applications.
Degree: PhD, 2017, University of Bath
URL: https://researchportal.bath.ac.uk/en/studentthesis/printing-materials-and-processes-for-electrochemical-applications(f347c391-da75-48e7-a208-f0191c55c006).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715249
► 3D printing has revolutionised traditional manufacturing methods, opening up and distributing design and production of low cost, custom objects to virtually anyone. Tailoring of print…
(more)
▼ 3D printing has revolutionised traditional manufacturing methods, opening up and distributing design and production of low cost, custom objects to virtually anyone. Tailoring of print material and part geometry allows for the benefits of this technology to reach multiple engineering and scientific fields, given appropriate design. A multidisciplinary approach concerning development of new print materials and methods was undertaken with the aim of further expansion and application of 3D printing towards electrochemical applications. Specific requirements of materials used in this domain, such as conductivity and chemical stability, led to development of functional printable carbon composites, compatible with consumer grade 3D printers. This allows facile production of cheap, reusable, disposable, electrodes for analytical applications, demonstrating heavy metal detection in aqueous media and allowing further tailoring to specific applications to be easily implemented. A new method for printing of cellulose solutions was developed, with post processing of printed parts resulting in biocompatible, porous, conductive structures. When used as electrodes in microbial fuel cells, improved power and current output over traditionally used carbon cloth electrodes was achieved. Other developments resulting from this work applicable to other fields include a novel trajectory generation method based on exponential functions which can be applied to practically any robotic system, as well as improvements to the production process of metal alloy filaments for 3D printing of metallic components.
Subjects/Keywords: 620.1; 3D printing; Additive manufacturing; Microbial fuel cell; Electroanalysis
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rymansaib, Z. (2017). Printing materials and processes for electrochemical applications. (Doctoral Dissertation). University of Bath. Retrieved from https://researchportal.bath.ac.uk/en/studentthesis/printing-materials-and-processes-for-electrochemical-applications(f347c391-da75-48e7-a208-f0191c55c006).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715249
Chicago Manual of Style (16th Edition):
Rymansaib, Zuhayr. “Printing materials and processes for electrochemical applications.” 2017. Doctoral Dissertation, University of Bath. Accessed April 14, 2021.
https://researchportal.bath.ac.uk/en/studentthesis/printing-materials-and-processes-for-electrochemical-applications(f347c391-da75-48e7-a208-f0191c55c006).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715249.
MLA Handbook (7th Edition):
Rymansaib, Zuhayr. “Printing materials and processes for electrochemical applications.” 2017. Web. 14 Apr 2021.
Vancouver:
Rymansaib Z. Printing materials and processes for electrochemical applications. [Internet] [Doctoral dissertation]. University of Bath; 2017. [cited 2021 Apr 14].
Available from: https://researchportal.bath.ac.uk/en/studentthesis/printing-materials-and-processes-for-electrochemical-applications(f347c391-da75-48e7-a208-f0191c55c006).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715249.
Council of Science Editors:
Rymansaib Z. Printing materials and processes for electrochemical applications. [Doctoral Dissertation]. University of Bath; 2017. Available from: https://researchportal.bath.ac.uk/en/studentthesis/printing-materials-and-processes-for-electrochemical-applications(f347c391-da75-48e7-a208-f0191c55c006).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715249
Clemson University
14.
Shuford, Stephen.
INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS.
Degree: MS, Bioengineering, 2012, Clemson University
URL: https://tigerprints.clemson.edu/all_theses/1407
► ABSTRACT We will review the conversion of a standard thermal inkjet printer into a bioprinting system and the effects of printing F-actin monomers with cells.…
(more)
▼ ABSTRACT We will review the conversion of a standard thermal inkjet printer into a bioprinting system and the effects of
printing F-actin monomers with cells. The use of any
printing system along with biological material or for biological or medical use has been termed bioprinting. Bioprinting has been used in vascular grafts, scaffold design, gene transfection, micro patterning and many other applications and is very diverse. Specifically we will look at the internalization of F-actin monomers into 3T3 fibroblasts as a result of
cell membrane disruption from thermal inkjet
printing. If the actin monomers were internalized and then incorporated into the cytoskeleton, further investigation of cytoskeleton organization, construction and response to mechanical loading from atomic for microscopy could be conducted. First, a bioprinter had to be modified from a standard printer. An HP Deskjet 500C and an HP Deskjet 500 were used. The only difference is that the HP Deskjet 500C is a color printer and has a different type of cartridge. Both the printers themselves and the ink cartridges that accompanied them had to be modified to accommodate cells and F-actin monomer solution. The printer and cartridges were customized for the application of
printing cells. A proof of concept was performed first to see if the converted HP Deskjet 500 could indeed print viable cells without any marked decrease in viability and function. After finding that the cells that were being printed were not only viable, but also continued to grow until confluence it was decided to print the cells along with the fluorescently tagged F-actin monomers to see if monomers could be internalized by the printed cells. Fluorescence microscopy of the confirmed that the monomers could be internalized by the
cell before the damage to the
cell membrane could be repaired.
Advisors/Committee Members: Dean, Delphine, Nagatomi , Jiro, Webb , Ken.
Subjects/Keywords: actin; bioprinting; cytoskeleton; firboblasts; thermal inkjet printing; transfection; Cell Biology
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Export
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APA (6th Edition):
Shuford, S. (2012). INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS. (Masters Thesis). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_theses/1407
Chicago Manual of Style (16th Edition):
Shuford, Stephen. “INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS.” 2012. Masters Thesis, Clemson University. Accessed April 14, 2021.
https://tigerprints.clemson.edu/all_theses/1407.
MLA Handbook (7th Edition):
Shuford, Stephen. “INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS.” 2012. Web. 14 Apr 2021.
Vancouver:
Shuford S. INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS. [Internet] [Masters thesis]. Clemson University; 2012. [cited 2021 Apr 14].
Available from: https://tigerprints.clemson.edu/all_theses/1407.
Council of Science Editors:
Shuford S. INTERNALIZATION OF F-ACTIN MONOMERS INTO 3T3 FIBROBLASTS VIA THERMAL INKJET PRINTING FOR INVESTIGATION OF CYTOSKELETON INCORPORATION AND MECHANICS. [Masters Thesis]. Clemson University; 2012. Available from: https://tigerprints.clemson.edu/all_theses/1407
University of New South Wales
15.
Ngalim, Siti Hawa.
Cell migration in the context of immobilised and soluble cues.
Degree: Centre for Vascular Research, 2013, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/52893
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11571/SOURCE01?view=true
► Cells respond to adhesive and soluble cues in the extracellular matrix by modulating their behaviour including cell adhesion, migration speed and direction. In vivo, cells…
(more)
▼ Cells respond to adhesive and soluble cues in the extracellular matrix by modulating their behaviour including
cell adhesion, migration speed and direction. In vivo, cells are exposed to multiple, even conflicting directional cues, however, how
cell migration is influenced by multiple spatial cues is still unclear. The effects of adhesive and soluble cues on
cell adhesion and migration have often been studied separately. In this thesis, surface-patterning techniques and microfluidics are combined to independently present adhesive and soluble cues. Microcontact
printing was used to pattern adhesive cues onto a layer of polyethylene glycol (PEG) that passivated the underlying surfaces. A microfluidic device was employed to deliver a gradient of soluble cues to migrating cells. Migration of Hela cells derived from human cervical cancer and J774 cells, immortalized mouse macrophages, was recorded with live-
cell fluorescence microscopy and analysis by single
cell tracking. In the first chapter reporting results (Chapter 3), surface passivation and surface patterning of fibronectin via microcontact
printing were established and
cell migration was observed with a modified wound assay. It is shown that HeLa cells required fibronectin for adhesion and migration and did not migrate into passivated PEG regions of the surface. Only when the
cell density on the fibronectin tracks was high did individual HeLa cells migrate into PEG regions. Conversely, J774 cells migrated faster on PEG regions than on fibronectin tracks. The distinct
cell morphology indicated that J774 may adopt different migrating modes on fibronectin and PEG surfaces. In Chapter 4, the modified surfaces from Chapter 3 were integrated with a microfluidic device to create a gradient of fetal bovine serum (FBS) or complement 5a (C5a) as a soluble cue for chemotaxis. Hence this chapter describes the role of patterned surfaces and directional cue of soluble gradients on chemotaxis. HeLa cells were found to migrate towards higher concentrations of FBS, but only when a fibronectin track was accessible in that direction. It was concluded that chemotaxis of HeLa cells is strongly adhesion dependent. In contrast, J774 cells migrated faster and more directed towards the source of C5a on fibronectin tracks than on PEG regions, suggesting that chemotaxis of macrophages is enhanced on adhesive cues.In Chapter 5, two competing directional cues were presented to migrating cells. These were (i) gradients of arginine-glycine-aspartic acid (RGD) peptides immobilised onto streptavidin tracks printed on PEG passivated surfaces and (ii) soluble chemoattractant gradient introduced with the microfluidic device used in Chapter 4. When RGD gradients and FBS gradients opposed each other, a greater fraction of HeLa cells was found to migrate towards the source of FBS rather than towards higher concentrations of RGD peptides. Hence for HeLa cells, in the presence of any adhesive cues, soluble cues were dominant in determining the direction of migration. J774 cells on RGD gradients and PEG…
Advisors/Committee Members: Boecking, Till, Medical Sciences, Faculty of Medicine, UNSW, Gaus, Katharina, Medical Sciences, Faculty of Medicine, UNSW, Gooding, J Justin, Chemistry, Faculty of Science, UNSW.
Subjects/Keywords: Microfluidic; Cell migration; Microcontact printing; Soluble cue; Adhesive cue; Chemotaxis; Haptotaxis
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APA (6th Edition):
Ngalim, S. H. (2013). Cell migration in the context of immobilised and soluble cues. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52893 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11571/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Ngalim, Siti Hawa. “Cell migration in the context of immobilised and soluble cues.” 2013. Doctoral Dissertation, University of New South Wales. Accessed April 14, 2021.
http://handle.unsw.edu.au/1959.4/52893 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11571/SOURCE01?view=true.
MLA Handbook (7th Edition):
Ngalim, Siti Hawa. “Cell migration in the context of immobilised and soluble cues.” 2013. Web. 14 Apr 2021.
Vancouver:
Ngalim SH. Cell migration in the context of immobilised and soluble cues. [Internet] [Doctoral dissertation]. University of New South Wales; 2013. [cited 2021 Apr 14].
Available from: http://handle.unsw.edu.au/1959.4/52893 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11571/SOURCE01?view=true.
Council of Science Editors:
Ngalim SH. Cell migration in the context of immobilised and soluble cues. [Doctoral Dissertation]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52893 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11571/SOURCE01?view=true
University of Canterbury
16.
Lang, Michael.
Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs.
Degree: M. Eng., Mechanical Engineering, 2012, University of Canterbury
URL: http://dx.doi.org/10.26021/3003
► Tissue engineering is a field devoted to the design and creation of replacement tissues with the ultimate goal of one day providing replacement organs. Traditional…
(more)
▼ Tissue engineering is a field devoted to the design and creation of replacement
tissues with the ultimate goal of one day providing replacement organs. Traditional
strategies to accomplish this through the bulk seeding of cells onto a single
monolithic porous bio-scaffold are unable to realise a precise architecture, thus
the inability to mimic the cells natural micro-environment found within the body.
Bio-printing approaches are the current state of the art with the ability to
accurately mimic the complex 3D hierarchical structure of tissue. However, a
functional construct also requires high strength to provide adequate support in
load bearing applications such as bone and cartilage tissue engineering, and to
maintain the open geometry of a large intricate channel network, which is crucial
for the transport of nutrients and wastes. Typical approaches utilise materials
which have processing parameters more amendable for cell incorporation, thus
they can be simultaneously deposited with scaffolding material. However, the
resulting construct is typically of low strength.
This thesis explores the automation of a printing and “tissue assembly” process
with the ability to incorporate delicate cell aggregates or spheroids within a high
strength bio-scaffold requiring harsh processing parameters, at precise locations.
The 3D printed bio-scaffold has a lattice architecture which enables a frictional fit
to be formed between the particle and scaffold, thus preventing egress. To achieve
this the pore must be expanded before the delivery of a single 1mm particle.
Novel subsystems were developed to automate this process and provide the ability
to achieve scalable, flexible, complex constructs with accurate architecture.
A system architecture employing the benefits of modularity was devised. The
main subsystems developed were the singulation device, to ensure the separation
of a single particle; the injection device, to deliver and seed particles into the
scaffold, and the control system, to facilitate the operation of the devices.
Three generations of singulation devices have been developed ranging from
mechanical to fluid manipulation methods alone. The first prototype utilised
mechanical methods, with simple control methods. However the inability to
correctly position the lead particle within the singulation chamber, resulted in
damage to the test alginate particles. In the second prototype a fully fluidics based
device utilised two trapping sites to capture the leading particles. Singulation
success rates of up to 88% was achieved. Higher rates were limited by the trapped
particle’s interaction with the lagging particles during capture. In a similar
concept to the second prototype, the third prototype utilised only a single trapped
particle, and achieved much higher throughput, and 100% singulation accuracy.
The injection device, utilised a conical expanding rod within a thin outer
sheath. It was able to expand the pore, with minimal damage to the scaffold,
providing an unobstructed path…
Subjects/Keywords: Bio-printing; Tissue engineering; micro-assembly; cell singulation
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APA (6th Edition):
Lang, M. (2012). Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs. (Masters Thesis). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/3003
Chicago Manual of Style (16th Edition):
Lang, Michael. “Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs.” 2012. Masters Thesis, University of Canterbury. Accessed April 14, 2021.
http://dx.doi.org/10.26021/3003.
MLA Handbook (7th Edition):
Lang, Michael. “Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs.” 2012. Web. 14 Apr 2021.
Vancouver:
Lang M. Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs. [Internet] [Masters thesis]. University of Canterbury; 2012. [cited 2021 Apr 14].
Available from: http://dx.doi.org/10.26021/3003.
Council of Science Editors:
Lang M. Systems for the automated 3D assembly of micro-tissue and bio-printing of tissue engineered constructs. [Masters Thesis]. University of Canterbury; 2012. Available from: http://dx.doi.org/10.26021/3003
University of California – San Francisco
17.
Mendelsohn, Adam.
Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters.
Degree: Bioengineering, 2011, University of California – San Francisco
URL: http://www.escholarship.org/uc/item/2b8701k8
► Transplantation of encapsulated insulin-secreting cell clusters represents a potential cure for type I diabetes, but development efforts so far have yet to live up to…
(more)
▼ Transplantation of encapsulated insulin-secreting cell clusters represents a potential cure for type I diabetes, but development efforts so far have yet to live up to its promise. Recent studies have elucidated the importance of cluster size on the insulin response in a manner that affects viability and efficacy of transplanted clusters. Using microfabrication techniques, a method to fabricate uniformly-sized insulin-secreting cell clusters was developed and thoroughly characterized using water contact angle, x-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and fluorescent microscopy. This technique enabled the formation of both monolayered and multilayered cell clusters of a predetermined size and shape. Subsequent evaluation of the impact that cluster size has on insulin expression, content and secretion using RT-PCR, ELISA, and confocal quantitative immunocytochemistry suggested that two cluster-size dependent behavioral changes relevant to transplant efficacy exist: First, glucose stimulation causes increased insulin production for clusters exceeding 40 µm in size. Second, cluster sizes greater than 60 µm secrete insulin more efficiently after production than smaller sized clusters. These results suggest that an optimal cluster size exists between 100-120 µm. Lastly, human embryonic stem cells were differentiated in patterned 120 µm clusters along the pancreatic lineage, an effort that could produce optimally sized insulin-secreting cell clusters from a renewable cell supply. The studies presented here may help overcome two remaining challenges preventing encapsulated cell transplantation therapy from truly providing a cure for type I diabetes.
Subjects/Keywords: Biomedical Engineering; Materials Science; Cell Patterning; Diabetes; Islet; Microcontact Printing; Stem Cell Differentiation
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APA (6th Edition):
Mendelsohn, A. (2011). Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/2b8701k8
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mendelsohn, Adam. “Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters.” 2011. Thesis, University of California – San Francisco. Accessed April 14, 2021.
http://www.escholarship.org/uc/item/2b8701k8.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mendelsohn, Adam. “Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters.” 2011. Web. 14 Apr 2021.
Vancouver:
Mendelsohn A. Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters. [Internet] [Thesis]. University of California – San Francisco; 2011. [cited 2021 Apr 14].
Available from: http://www.escholarship.org/uc/item/2b8701k8.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mendelsohn A. Development of a Bioartificial Pancreas Using Size-Controlled Insulin-Secreting Cell Clusters. [Thesis]. University of California – San Francisco; 2011. Available from: http://www.escholarship.org/uc/item/2b8701k8
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Texas – Austin
18.
Robinson, Michael Mayes.
Applications of micro-3D printing to microfluidic cell dosing.
Degree: PhD, Biomedical Engineering, 2014, University of Texas – Austin
URL: http://hdl.handle.net/2152/25912
► Cellular growth, development, differentiation, and death are mediated to some degree by the interaction of soluble factors with plasma membrane receptors. Traditionally the cellular response…
(more)
▼ Cellular growth, development, differentiation, and death are mediated to some degree by the interaction of soluble factors with plasma membrane receptors. Traditionally the cellular response to chemical cues has been studied by exposing entire culture dishes to a desired reagent. While the addition of soluble reagents homogenously to
cell culture dishes provides a basis for understanding much of
cell biology, greater spatial resolution of reagent delivery is necessary in order to elucidate mechanisms on the subcellular scale. This dissertation explores techniques that may improve the quality and precision of delivering soluble factors to cultured cells in order to better understand the complex processes of
cell biology. These advancements were made possible by applying high intensity, focused laser light to soluble materials to achieve microscopic three-dimensional (µ-3D)
printing. In combination with a previously developed microfluidic
cell dosing platform, microstructures were designed and µ-3D printed to hydrodynamically focus reagent streams for
cell dosing. Structures were also µ-3D printed within micrometers of living cells from a solution of gelatin and bovine serum albumin with minimal cytotoxicity. When µ-3D printed, these proteins displayed both temperature and pH-responsive properties. In order to allow for on-the-fly control of reagent stream size and temporal pulse width, microstructures were µ-3D printed from temperature-responsive N- isoproplyacrylamide. To further improve the temporal resolution of the system, a technique for cycling between reagents with millisecond exchange times using laminar flow microfluidics was developed. The utility of these techniques was demonstrated by staining rat Schwann cells and mouse neuroblastoma rat glioma hybrid cells (NG108-15) with focused streams of fluorescent dyes. These advancements may allow future experiments to determine the placement of soluble factors necessary for bacterial quorum sensing or stem
cell differentiation.
Advisors/Committee Members: Shear, Jason B. (advisor).
Subjects/Keywords: 3D printing; Microfluidic; Multiphoton; Hydrodynamic focusing; Cell dosing; Thermoresponsive; N-isoproplyacrylamide; Gelatin; Schwann cell; NG108-15 cell
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Robinson, M. M. (2014). Applications of micro-3D printing to microfluidic cell dosing. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/25912
Chicago Manual of Style (16th Edition):
Robinson, Michael Mayes. “Applications of micro-3D printing to microfluidic cell dosing.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed April 14, 2021.
http://hdl.handle.net/2152/25912.
MLA Handbook (7th Edition):
Robinson, Michael Mayes. “Applications of micro-3D printing to microfluidic cell dosing.” 2014. Web. 14 Apr 2021.
Vancouver:
Robinson MM. Applications of micro-3D printing to microfluidic cell dosing. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/2152/25912.
Council of Science Editors:
Robinson MM. Applications of micro-3D printing to microfluidic cell dosing. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/25912
NSYSU
19.
Jian, Siang-he.
The fabrication of a heterogeneous composite bipolar plate with 3D printing technique.
Degree: Master, Mechanical and Electro-Mechanical Engineering, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219114-190522
► Bipolar plates used in a PEMFC must have high electric conductivity, good mechanical and chemical stability, low gas permeability, and low cost. For portable applications,…
(more)
▼ Bipolar plates used in a PEMFC must have high electric conductivity, good mechanical and chemical stability, low gas permeability, and low cost. For portable applications, light weight and low volume should also be considered. Our laboratory has developed a heterogeneous composite bipolar plate, which has many advantages such as low contact resistance, good chemical stability, low cost and lightweight.
The laboratory used to produce bipolar plate with injection molding technology. It needs a long development time and the cost is high. 3D
printing technology is adopted in this research. The development time and cost are lowed of the bipolar plates and the complexity of the parts increased.
To produce more parts while enhancing their quality within the limited space inside the 3D
printing machine are studied in this research. Parts made by 3D
printing together with the laboratory produced carbon fiber were assembled to produce the heterogeneous composite bipolar plates. Subsequently, fuel
cell stack composed of 6 cells is fabricated with the bipolar plates and its performance evaluated.
Advisors/Committee Members: Ming-San Lee (committee member), Long-Jeng Chen (chair), Chorng-Fuh Liu (chair).
Subjects/Keywords: Heterogeneous composite bipolar plate; 3D Printer; fuel cell stack; fiber; the parts by 3D printing
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APA (6th Edition):
Jian, S. (2014). The fabrication of a heterogeneous composite bipolar plate with 3D printing technique. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219114-190522
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jian, Siang-he. “The fabrication of a heterogeneous composite bipolar plate with 3D printing technique.” 2014. Thesis, NSYSU. Accessed April 14, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219114-190522.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jian, Siang-he. “The fabrication of a heterogeneous composite bipolar plate with 3D printing technique.” 2014. Web. 14 Apr 2021.
Vancouver:
Jian S. The fabrication of a heterogeneous composite bipolar plate with 3D printing technique. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Apr 14].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219114-190522.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jian S. The fabrication of a heterogeneous composite bipolar plate with 3D printing technique. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0219114-190522
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
20.
Shukla, Shantanu T.
Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes.
Degree: PhD, Department of Mechanical Engineering, 2016, University of Alberta
URL: https://era.library.ualberta.ca/files/cbk128b067
► The success of commercial applicability of polymer electrolyte fuel cells (PEFCs) depends on the cost competitiveness with respect to current energy sources. A major fraction…
(more)
▼ The success of commercial applicability of polymer
electrolyte fuel cells (PEFCs) depends on the cost competitiveness
with respect to current energy sources. A major fraction of the
system cost can be mitigated by reducing the amount of platinum
(Pt) catalyst in the electrodes and by improving the catalyst
utilization. The electrode fabrication process governs the
electrode microstructure and Pt loading. Application of inkjet
printing (IJP) to PEFC electrode fabrication is a relatively recent
introduction and has not been extensively studied. The
drop-on-demand nature of this method allows for a precise control
over the deposition process, thickness and Pt loading of the
electrodes. A detailed analysis of this method is therefore
essential to understand its feasibility as a fabrication tool. In
this work, a comprehensive analysis of inkjet printed electrodes
has been carried out. By studying the effect of Nafion (from 10 wt%
to 50 wt%) in thin, low Pt loading IJP electrodes, the performance
is not found to be affected by Nafion loading in the range of 20 -
40 wt%. The effect of Pt loading on active area, Tafel slope,
reaction order and evaluation of oxygen transport resistance for
IJP electrodes has been carried out to understand their lower
performance compared to a conventional spray coated electrode. The
reduced performance of IJP electrodes at higher Pt loadings is
associated to a reduction in the active area and porosity. In an
attempt to improve reactant transport, a novel electrode coated
membrane (ECM) architecture is developed where the carbon
micro-porous layer (MPL) is fabricated directly over the catalyst
layer. Results indicated a lower transport resistance in ECMs as
compared to using an MPL based diffusion media. Inkjet printing is
also implemented to study patterned electrode structures. However,
fine enough patterns to show the advantage of patterned structures
could not be observed.Lastly, a study of particle interactions
using colloidal science is carried out to understand the effect of
dispersion solvents on ink stability. A semi-empirical model based
on diffusion limited aggregation is developed to evaluate the rate
of particle aggregation and predict the stability time.
Experimental determination of stability was carried out for carbon
based inks in non-aqueous dispersion media based on visual
inspection and measurement of particle size by dynamic light
scattering. A qualitative comparison of the stability time between
the model and experimental observation could be made. Overall, this
work presents an improved performance of IJP electrodes compared to
previous literature, possible reasons for the reduced performance
compared to conventional electrodes based on detailed analysis of
electrode parameters, a novel electrode coated membrane
architecture using IJP that improves the transport resistance and a
simple semi-empirical model for determination of ink dispersion
stability.
Subjects/Keywords: Electrode fabrication; fuel cell testing; catalyst ink dispersion; inkjet printing; colloidal interactions
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APA ·
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Manager
APA (6th Edition):
Shukla, S. T. (2016). Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cbk128b067
Chicago Manual of Style (16th Edition):
Shukla, Shantanu T. “Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes.” 2016. Doctoral Dissertation, University of Alberta. Accessed April 14, 2021.
https://era.library.ualberta.ca/files/cbk128b067.
MLA Handbook (7th Edition):
Shukla, Shantanu T. “Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes.” 2016. Web. 14 Apr 2021.
Vancouver:
Shukla ST. Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes. [Internet] [Doctoral dissertation]. University of Alberta; 2016. [cited 2021 Apr 14].
Available from: https://era.library.ualberta.ca/files/cbk128b067.
Council of Science Editors:
Shukla ST. Experimental Analysis of Inkjet Printed Polymer Electrolyte
Fuel Cell Electrodes. [Doctoral Dissertation]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/cbk128b067
Rochester Institute of Technology
21.
Hurst, Wilson.
Direct diffusion resist for gravure.
Degree: School of Print Media (CIAS), 1982, Rochester Institute of Technology
URL: https://scholarworks.rit.edu/theses/3886
► One of the most attractive attributes of the gravure process is its ability to print a variable ink film by virtue of cell depth variation.…
(more)
▼ One of the most attractive attributes of the gravure
process is its ability to print a variable ink film by
virtue of
cell depth variation. This
cell depth variation
is achieved through the use of a diffusion resist. All
diffusion resists are negative-working and, after exposure,
require transfer to plate or cylinder. A "negative-working"
resist is one where the emulsion becomes insoluble to the
developing solution in those areas struck by actinic
radiation. Presently available resists, which are directly
applied to plate or cylinder prior to exposure, are not
capable of controlling
cell depth variation. Thus, the tone
range obtainable in the print is limited. This sacrifice
has been justified by advantages inherent in the direct
system, such as ease of application and seamless image
capability. It has long been recognized, however, that a
direct system capable of variable
cell depth control would
be most advantageous.
The purpose of this study is to examine the possible
application of a radiation-sensitive/positive-working iron
salt system for use as a direct diffusion resist.
"Positive-working" refers to an emulsion which becomes
soluble in those areas struck by actinic radiation. The
is sensitized by immersing it in a potassium dichromate
solution, and is then dried in the dark. The concentration
of dichromate ranges from 2.5 to 5 percent. The time of
immersion varies with the temperature, the pH, and the
strength of the bath. The water content of the gelatin
before sensitizing is also most important. In general
practice, gravure tissues are immersed in the dichromate
bath for approximately three minutes.
"Carbon tissue is a long-scale process which can
accurately reproduce a tone range of 1.30 to as much as 1.50
without going into the under- or over-exposed regions of the
2
photographic reproduction curve." Despite many disadvan
tages associated with the instability of dichromated colloid
layers, no other radiation-sensitive material has been
found, up to the present, which can do the job so well.
The sensitized and dried pigment paper is then exposed
behind a photogravure screen which consists of opaque
squares separated by narrow, clear lines (about 150 to 175
lines to the inch). The clear lines are usually about 1/3
the width of the opaque squares which they separate. The
effect of this screen exposure is to form a network of
insoluble lines of hardened gelatin. The next step is to
print the screened tissue behind a continuous- tone positive
transparency. This results in a negative resist in which
the shadows are represented by gelatin hardened to only a
historical roots of this concept are explored and the
results of empirical tests explained. It is concluded that
the relief characteristics of such a resist system are
manifest in the solarization region of the characteristic
curve.
Advisors/Committee Members: Silver, Julius.
Subjects/Keywords: Intaglio printing; Gravure; Cell depth variation
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hurst, W. (1982). Direct diffusion resist for gravure. (Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/3886
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hurst, Wilson. “Direct diffusion resist for gravure.” 1982. Thesis, Rochester Institute of Technology. Accessed April 14, 2021.
https://scholarworks.rit.edu/theses/3886.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hurst, Wilson. “Direct diffusion resist for gravure.” 1982. Web. 14 Apr 2021.
Vancouver:
Hurst W. Direct diffusion resist for gravure. [Internet] [Thesis]. Rochester Institute of Technology; 1982. [cited 2021 Apr 14].
Available from: https://scholarworks.rit.edu/theses/3886.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hurst W. Direct diffusion resist for gravure. [Thesis]. Rochester Institute of Technology; 1982. Available from: https://scholarworks.rit.edu/theses/3886
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Texas A&M University
22.
Omoragbon, Oghogho Cynthia.
Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications.
Degree: MS, Mechanical Engineering, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/174093
► Porous metals play essential roles in lowering the value of elastic modulus, achieving a modulus like that of the human body. Solid freeform fabrication has…
(more)
▼ Porous metals play essential roles in lowering the value of elastic modulus, achieving a modulus like that of the human body. Solid freeform fabrication has the potential to overcome the limitations of traditional manufacturing methods with controlled internal pore architecture. The use of 3D
printing has been solely focused on polymer materials with limited investigations on producing porous metallic parts, such as implants. This research studies the influence of 3D
printing on the pore architecture and its impact on
cell growth. Selective laser melting (SLM), an additive manufacturing process, was used to fabricate 316L stainless steel porous structures. The stainless steel was selected for this research as a model system due to its known properties.
Four cubic models of 15×15×4 mm
3 were designed using Autodesk inventor with interconnected pore sizes of 0.4 mm, 0.6 mm, 0.8 mm, and 1.0 mm. The CAD files were converted to STL models and then extracted into QuantAM software, which produces
printing instructions for the Renishaw AM400 SLM machine. The results show that 316L stainless steel porous structures with fully interconnected pores were successfully fabricated using the SLM process, with a mean pore size reduction of 0.220 mm for all samples.
The samples were subjected to an accelerated corrosion test for 336 hours using the salt spray chamber. 5 % wt. of NaCl formed part of the composition of the corrosive media, and by comparing the corrosion rates and weight loss of the interconnected structures, the samples experienced an insignificant percentage weight loss and an average corrosion rate of 3.0 mpy.
The
cell culture experiment reveals the
cell growth viability of all samples of the selective-laser-melted 316L stainless steel structures seeded with Pseudomonas aeruginosa cells, for pore
sizes ranging between 0.182 mm to 0.783 mm. The 0.783 mm porous structure with the highest porosity of 61.2% was most conducive to biofilm formation, allowing
cell ingrowth into the pores.
Advisors/Committee Members: Liang, Hong (advisor), Ma, Chao (advisor), Pate, Michael (committee member).
Subjects/Keywords: 3D printing; Scaffolds; Cell growth; Additive Manufacturing; Selective Laser Melting Technique; 316L Stainless steel
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APA (6th Edition):
Omoragbon, O. C. (2018). Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/174093
Chicago Manual of Style (16th Edition):
Omoragbon, Oghogho Cynthia. “Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications.” 2018. Masters Thesis, Texas A&M University. Accessed April 14, 2021.
http://hdl.handle.net/1969.1/174093.
MLA Handbook (7th Edition):
Omoragbon, Oghogho Cynthia. “Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications.” 2018. Web. 14 Apr 2021.
Vancouver:
Omoragbon OC. Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications. [Internet] [Masters thesis]. Texas A&M University; 2018. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1969.1/174093.
Council of Science Editors:
Omoragbon OC. Fabrication and Characterization of Porous 316L Stainless Steel Using Selective Laser Melting Technique for Biomedical Applications. [Masters Thesis]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/174093
Iowa State University
23.
Lara-Rodriguez, Laura M.
Design and fabrication of engineered platforms to control multiple cue directed cell migration.
Degree: 2014, Iowa State University
URL: https://lib.dr.iastate.edu/etd/14186
► Cell migration is essential during a variety of physiological events, and as such it has been extensively studied in an attempt to unravel the details…
(more)
▼ Cell migration is essential during a variety of physiological events, and as such it has been extensively studied in an attempt to unravel the details of its mechanisms. Cells receive information from the environment and migrate accordingly. Within the last few decades, different studies have evaluated the effect of different types of signals and the way in which they regulate cell migration. However, cells do not respond to individual cues, but they are constantly receiving information from a variety of signals in vivo. One particular scenario in which the concerted action of multiple signals is thought to be relevant is during the early stages of cancer invasion and metastasis. Different studies suggest that the architecture of the tissue surrounding the tumor guides the migration of the cells to blood vessels in the vicinity, initiating the metastatic cascade. Other evidence suggests the role of a variety of chemokines that can also direct cancer cell invasion. In that regard, the main goal of this research is to analyze the response of a highly metastatic cell line to contact guidance and chemotactic signals when they are presented simultaneously. To achieve this purpose we develop 2 different platforms that combine microcontact printed surfaces with the Dunn chemotaxis chamber and an agarose sphere assay. Our results provide evidence to suggest that the synergy of these two signals increases the efficiency of cell migration during metastatic invasion.
Subjects/Keywords: Chemical Engineering; Chemotaxis; Contact Guidance; Microcontact Printing; Migration; Biomedical; Cell Biology; Chemical Engineering
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APA (6th Edition):
Lara-Rodriguez, L. M. (2014). Design and fabrication of engineered platforms to control multiple cue directed cell migration. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/14186
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lara-Rodriguez, Laura M. “Design and fabrication of engineered platforms to control multiple cue directed cell migration.” 2014. Thesis, Iowa State University. Accessed April 14, 2021.
https://lib.dr.iastate.edu/etd/14186.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lara-Rodriguez, Laura M. “Design and fabrication of engineered platforms to control multiple cue directed cell migration.” 2014. Web. 14 Apr 2021.
Vancouver:
Lara-Rodriguez LM. Design and fabrication of engineered platforms to control multiple cue directed cell migration. [Internet] [Thesis]. Iowa State University; 2014. [cited 2021 Apr 14].
Available from: https://lib.dr.iastate.edu/etd/14186.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lara-Rodriguez LM. Design and fabrication of engineered platforms to control multiple cue directed cell migration. [Thesis]. Iowa State University; 2014. Available from: https://lib.dr.iastate.edu/etd/14186
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Cambridge
24.
Gill, Elisabeth Lauren.
Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.
Degree: PhD, 2020, University of Cambridge
URL: https://www.repository.cam.ac.uk/handle/1810/303298
► Lab-grown tissues have tremendous potential to accelerate drug discovery and identify some of the underlying mechanisms behind diseases. The native extracellular matrix (ECM) of tissues…
(more)
▼ Lab-grown tissues have tremendous potential to accelerate drug discovery and identify some of the underlying mechanisms behind diseases. The native extracellular matrix (ECM) of tissues is a complex, hierarchical fibrous protein structure with delicate mechanical properties that guides tissue assembly and regeneration. Existing biomaterial fabrication techniques struggle to simultaneously attain: micro/nano-scale fibril feature resolution, low bulk stiffness and the 3D organisation crucially provided by the ECM without comprising cell motility. This work utilises 3D printing and low voltage electrospinning patterning synergistically to address these conflicting engineering challenges and act as a minimalist guide for 3D cell growth. A version of low voltage electrospinning patterning was adapted as a sequential process on a modified 3D printer. Applied voltage and 3D printed geometry can modulate the suspended behaviour of electrospun fibres that span between 3D printed support pillars, a parametric study characterised threshold conditions and established a predictive model for patterning suspended fibres. The accuracy with which suspended fibres followed the in-plane tool path was also assessed. Scanning Electron Microscopy imaging measured fibre diameters 1-5 μm and mechanical testing examines the properties for a given layer of dry fibres. The configuration demonstrated unique patterning of stacked suspended fibre layers in multiple orientations. Tissue scaffolding applications were explored in 2D and 3D. In 2D, gelatin fibres were patterned as a topographic cue to direct mesenchymal stem cells towards the osteogenic lineage. For 3D cell culture, the use of suspended fibre devices was investigated to improve the efficiency of cerebral organoid assembly. Pursuing these applications led to further refinement of the fibre fabrication technique and the development of targeted cell seeding strategies on suspended fibre structures. Glioblastoma cell aggregates were cultured on suspended fibre devices. Fibres guided the outgrowth of cancer cells from the aggregates, mimicking the topography of white matter tracts that assist migration in vivo. Cells assemble into dense (~200 μm depth) tissue structures with necrotic cores, that can remodel the fibre network yet are guided by the underlying fibre organisation. This novel method of patterning suspended microfibres from solution offers several avenues of inquiry to mimic ECM topography and complex material functionality.
Subjects/Keywords: electrospinning; biofabrication; 3d printing; 3d cell culture; suspended fibre patterning; low voltage
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APA ·
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MLA ·
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APA (6th Edition):
Gill, E. L. (2020). Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/303298
Chicago Manual of Style (16th Edition):
Gill, Elisabeth Lauren. “Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.” 2020. Doctoral Dissertation, University of Cambridge. Accessed April 14, 2021.
https://www.repository.cam.ac.uk/handle/1810/303298.
MLA Handbook (7th Edition):
Gill, Elisabeth Lauren. “Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly.” 2020. Web. 14 Apr 2021.
Vancouver:
Gill EL. Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Apr 14].
Available from: https://www.repository.cam.ac.uk/handle/1810/303298.
Council of Science Editors:
Gill EL. Fabrication of Designable and Suspended Microfibres via Low Voltage Electrospinning Patterning towards Replicating Extracellular Matrix Cues for Tissue Assembly. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://www.repository.cam.ac.uk/handle/1810/303298
Univerzitet u Beogradu
25.
Vasiljević, Zorka Ž., , 35406439.
Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5.
Degree: Tehnološko-metalurški fakultet, 2020, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:21082/bdef:Content/get
► Tehnološko inženjerstvo - Inženjerstvo materijala / Technological Engineering - Materials Engineering
U poređenju sa fotonaponskim ćelijama, fotoelektrohemijske ćelije predstavljaju efikasan vid pretvaranja sunčeve energije u…
(more)
▼ Tehnološko inženjerstvo - Inženjerstvo materijala /
Technological Engineering - Materials Engineering
U poređenju sa fotonaponskim ćelijama,
fotoelektrohemijske ćelije predstavljaju efikasan vid pretvaranja
sunčeve energije u električnu, jer se pored energije dobija i
vodonik koji se može iskoristiti kao gorivo. Fotoelektrohemijske
ćelije sastoje se od poluprovodničke anode (p- ili n-tipa) koja
apsorbuje svetlost, pri čemu se konverzija energije odigrava na
granici faza poluprovodnik-elektrolit. U ovoj doktorskoj
disertaciji primenjena su tri istraživačka pravca za dobijanje
filmova u svojstvu fotoaktivnih anoda. Prvi istraživački pravac bio
je priprema nanočestičnog poluprovodnika - pseudobrukita, Fe2TiO5,
reakcijom u čvrstom stanju između anatasa, TiO2 i hematita,
α-Fe2O3. Analiza dobijanja najpre je praćena na tabletama radi
optimizacije sastava, temperature i vremena sinterovanja. Ispitan
je uticaj promene temperature sinterovanja (750 – 1250 C) na fazni
sastav, temperaturu faznog prelaza, morfologiju, kao i električna
svojstva sinterovanih uzoraka. Izabrane su dve oksidne smeše
maseni odnos prahova 40%α-Fe2O3/60%TiO2 (4F6T) i 60%α-Fe2O3/40%TiO2
(6F4T) što približno odgovara molskom odnosu početnih prahova
hematita i anatasa od 1:3 i 1,5:2, sa ciljem da se dobije
pseudobrukit i analizira uticaj viška anatasa. Uzorci sinterovani
na temperaturi 850 C sadržali su rutil i pseudobrukit sa rombičnom
strukturom čime je potvrđeno da gvožđe ubrzava faznu transformaciju
anatasa u rutil pri čemu se formira i pseudobrukitna faza. Dalje
povišenje temperature uticalo je na povećanje gustine uzoraka,
promene u veličini zrna i smanjenje električne otpornosti. Drugi
istraživački pravac bio je priprema elektrodnih filmova
sitoštampanjem pasti koje su dobijene mešanjem homogenizovanih
prahova sa organskom smolom (butil celuloza), rastvaračem
(terpinol) i malom količinom veziva – ROSiO2B2O3 staklo (RO-oksid
retkih zemalja). Dobijene paste deponovane su sitoštampom na
supstrat od alumine i potom sinterovane u hibridnoj peći 60 minuta
u temperaturnom opsegu 800 – 950 C. Rendgenskom difrakcionom
analizom utvrđeno je da se na 800 C formira monoklinični
pseudobrukit i da je završena fazna transformacija anatasa u rutil,
kao i da je dalje povišenje temperature utiče na prelaz
monokliničnog u rombični pseudobrukit. Uzorci sinterovani na 850 C
imali su malu veličinu zrna sa homogenom strukturom, dok je dalje
povišenje temperature uticalo na formiranje pseudobrukita sa većim
zrnima štapićastog oblika. Strujno-naponska analiza pokazala je da
uzorak 60%α-Fe2O3/40%TiO2 sinterovan na 850 C ima mogućnost za
primenu u fotoelektrohemijskim ćelijama...
Advisors/Committee Members: Rogan, Jelena, 1971-, 12829543.
Subjects/Keywords: iron(III) oxide; titanium(IV) oxide; iron(III)
titanate; photoanode; screen printing; photoelectrochemical
cell
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APA ·
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APA (6th Edition):
Vasiljević, Zorka Ž., , 3. (2020). Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:21082/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vasiljević, Zorka Ž., , 35406439. “Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5.” 2020. Thesis, Univerzitet u Beogradu. Accessed April 14, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:21082/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vasiljević, Zorka Ž., , 35406439. “Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5.” 2020. Web. 14 Apr 2021.
Vancouver:
Vasiljević, Zorka Ž., 3. Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5. [Internet] [Thesis]. Univerzitet u Beogradu; 2020. [cited 2021 Apr 14].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:21082/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vasiljević, Zorka Ž., 3. Sinteza, struktura, karakterizacija i fotoelektrohemijska
primena debelih slojeva pseudobrukita, Fe2TiO5. [Thesis]. Univerzitet u Beogradu; 2020. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:21082/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Clemson University
26.
He, Ping.
Fluid Dynamics of Cell Printing.
Degree: PhD, Mechanical Engineering, 2011, Clemson University
URL: https://tigerprints.clemson.edu/all_dissertations/727
► Cell printing is an emerging technology that uses droplets to deliver cells to desired positions with resolution potentially comparable to the size of single…
(more)
▼ Cell printing is an emerging technology that uses droplets to deliver cells to desired positions with resolution potentially comparable to the size of single cells. In particular, ink–jet based
cell printing technique has been successfully used to build simple bio–constructs and has shown a promise in building complex bio–structures or even organs. Two important issues in ink–jet based
cell printing are the moderate survival rate of delicate cells and the limited
cell placement resolution. Resolving these issues is critical for the ink–jet based
cell printing techniques to realize their full potential. In this work, we use numerical simulations to reconstruct the impact of a droplet loaded with a single
cell onto a pool of viscous fluids to gain insights into the droplet and
cell dynamics during
cell printing. We developed a mathematical model for this process: the droplet, pool and air are modeled as Newtonian fluids, and their flow is modeled as a laminar flow governed by the Navier–Stokes equation. The
cell is modeled as an axisymmetric solid object governed by the neo–Hookean law and also has a shear viscosity that is the same as that of its host droplet. To numerically solve the coupled fluid and
cell motion, we used a hybrid method in which fluid flow is solved on a fixed Cartesian grid and the deformation of solid body is solved on a Lagrangian mesh. We also developed a new full Eulerian method, termed the solid level set (SLS) method, to simulate
cell printing. The key idea is to track the deformation of the solid body using four level set functions on a fixed Cartesian grid instead of using a Lagrangian mesh. The SLS method is easy to implement and addresses several challenges in simulations of fluid–tructure interactions using hybrid Eulerian/Lagrangian meshes. Using codes developed based on the above methods, we systematically investigated the fluid and
cell dynamics during the
cell printing process. We studied how the droplet penetration depth, droplet lateral spreading,
cell stress and
cell surface area change are affected by
printing conditions such as impact velocity, pool depth, and
cell stiffness. Our simulations indicate that
cell experiences significant stress (∼20kPa) and local surface area dilation (∼100%) during the impact process. The latter suggests that
cell membrane is temporally ruptured during the
printing process, and is consistent with the gene transfection observed during
cell printing. We speculate that the survival of
cell through the rather violent
cell printing process may be related to the briefness of the impact process, which only lasts about 0.1 milliseconds. Based on our simulation results, several strategies have been proposed to reduce the stress and membrane dilation of cells during
cell printing.
Advisors/Committee Members: Qiao, Rui, Tong , Chenning, Miller , Richard S, Zumbrunnen , David A.
Subjects/Keywords: cell printing; computational fluid dynamics; droplet impact; fluid struture interactions; multi-phase flow; Mechanical Engineering
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
He, P. (2011). Fluid Dynamics of Cell Printing. (Doctoral Dissertation). Clemson University. Retrieved from https://tigerprints.clemson.edu/all_dissertations/727
Chicago Manual of Style (16th Edition):
He, Ping. “Fluid Dynamics of Cell Printing.” 2011. Doctoral Dissertation, Clemson University. Accessed April 14, 2021.
https://tigerprints.clemson.edu/all_dissertations/727.
MLA Handbook (7th Edition):
He, Ping. “Fluid Dynamics of Cell Printing.” 2011. Web. 14 Apr 2021.
Vancouver:
He P. Fluid Dynamics of Cell Printing. [Internet] [Doctoral dissertation]. Clemson University; 2011. [cited 2021 Apr 14].
Available from: https://tigerprints.clemson.edu/all_dissertations/727.
Council of Science Editors:
He P. Fluid Dynamics of Cell Printing. [Doctoral Dissertation]. Clemson University; 2011. Available from: https://tigerprints.clemson.edu/all_dissertations/727
Wright State University
27.
Al-wattar, Tahseen Abdulridha Ali.
Developing equivalent solid model for lattice cell structure
using numerical approaches.
Degree: PhD, Engineering PhD, 2020, Wright State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=wright1610335304435815
► Lattice cell structures (LCS) are the engineered porous structures that are composed of periodic unit cells in three dimensions. Such structures have many scientific and…
(more)
▼ Lattice
cell structures (LCS) are the engineered
porous structures that are composed of periodic unit cells in three
dimensions. Such structures have many scientific and engineering
applications, such as in vessel gas technology, thermal systems,
mechanical and aerospace structures, etc. for which lightweight,
high strength, and energy absorption capabilities are essential
properties. To have an optimized design, finite element analysis
(FEA) based computational approach can be used for detailed
analysis of such structures, sometime in full scale. However,
developing a large-scale model for a lattice-based structure is
computationally expensive. If an equivalent solid FE model can be
developed using the equivalent solid mechanical properties of a
lattice structure, the computational time will be greatly reduced.
The main objective of this research is to develop a material model
which is equivalent to the mechanical response of a lattice
structure. In this study, the mechanical behavior of body centered
cubic (BCC) configuration and its derivative such as a BCC placed
inside boxed frame (here, termed as `InsideBCC’) under compression
and within elastic limit is considered. The BCC and InsideBCC
configurations are chosen because they provide the bounds of the
mechanical properties of LCS involving BCC derivatives. First, the
finite element analysis approach and theoretical calculations are
used on a single unit
cell of BCC and InsideBCC for several cases
(different strut diameters and
cell sizes) to predict equivalent
solid properties. The equivalent quasi-isotropic properties
required to describe the material behavior of both BCC and
InsideBCC unit cells are equivalent Young’s modulus (E_e),
equivalent shear modulus (G_e), and equivalent Poisson’s ratio
(ν_e). The results are then used to develop two separate neural
networks (NN) models so that the equivalent solid properties of a
BCC or InsideBCC lattice of any geometrical parameters can be
predicted. The input data of NN are bulk material properties and
geometrical parameters and output data are equivalent solid
mechanical properties. For each unit
cell configurations, two
separate FEA models are then developed for compression loading: (a)
one with 5 x 5 x 4
cell for BCC and the other with 6 x 6 x 4
cell
for InsideBCC, and (b) one completely solid with equivalent solid
properties obtained from NN. In addition, the BCC and InsideBCC LCS
specimens are fabricated on a Fused Deposition Modeling uPrint
SEplus 3D printer using Acrylonitrile Butadiene Styrene (ABS) and
tested under compression. Experimental load-displacement behavior
and the results obtained from both the FE models are in good
agreement with the experimental data within the elastic
limit.
Advisors/Committee Members: Mian, Ahsan (Advisor).
Subjects/Keywords: Mechanical Engineering; lattice cell structures; BCC unit cell; InsideBCC; equivalent solid properties; three-dimensional printing; Equivalent solid material model; Numerical Approaches
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Al-wattar, T. A. A. (2020). Developing equivalent solid model for lattice cell structure
using numerical approaches. (Doctoral Dissertation). Wright State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=wright1610335304435815
Chicago Manual of Style (16th Edition):
Al-wattar, Tahseen Abdulridha Ali. “Developing equivalent solid model for lattice cell structure
using numerical approaches.” 2020. Doctoral Dissertation, Wright State University. Accessed April 14, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=wright1610335304435815.
MLA Handbook (7th Edition):
Al-wattar, Tahseen Abdulridha Ali. “Developing equivalent solid model for lattice cell structure
using numerical approaches.” 2020. Web. 14 Apr 2021.
Vancouver:
Al-wattar TAA. Developing equivalent solid model for lattice cell structure
using numerical approaches. [Internet] [Doctoral dissertation]. Wright State University; 2020. [cited 2021 Apr 14].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1610335304435815.
Council of Science Editors:
Al-wattar TAA. Developing equivalent solid model for lattice cell structure
using numerical approaches. [Doctoral Dissertation]. Wright State University; 2020. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=wright1610335304435815
28.
LI ERQIANG.
The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing.
Degree: 2010, National University of Singapore
URL: http://scholarbank.nus.edu.sg/handle/10635/22832
Subjects/Keywords: Drop-on-demand; Inkjet printing; Interchangeable nozzle; Cell printing; Fine jetting
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❌
APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
ERQIANG, L. (2010). The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/22832
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
ERQIANG, LI. “The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing.” 2010. Thesis, National University of Singapore. Accessed April 14, 2021.
http://scholarbank.nus.edu.sg/handle/10635/22832.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
ERQIANG, LI. “The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing.” 2010. Web. 14 Apr 2021.
Vancouver:
ERQIANG L. The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing. [Internet] [Thesis]. National University of Singapore; 2010. [cited 2021 Apr 14].
Available from: http://scholarbank.nus.edu.sg/handle/10635/22832.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
ERQIANG L. The Generation And Experimental Study Of Microscale Droplets In Drop-On-Demand Inkjet Printing. [Thesis]. National University of Singapore; 2010. Available from: http://scholarbank.nus.edu.sg/handle/10635/22832
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Georgia Tech
29.
Lai, Jiun-Hong.
Development of low-cost high-efficiency commercial-ready advanced silicon solar cells.
Degree: PhD, Electrical and Computer Engineering, 2014, Georgia Tech
URL: http://hdl.handle.net/1853/52234
► The objective of the research in this thesis is to develop manufacturable high-efficiency silicon solar cells at low-cost through advanced cell design and technological innovations…
(more)
▼ The objective of the research in this thesis is to develop manufacturable high-efficiency silicon solar cells at low-cost through advanced
cell design and technological innovations using industrially feasible processes and equipment on commercial grade Czochralski (Cz) large-area (239 cm2) silicon wafers. This is accomplished by reducing both the electrical and optical losses in solar cells through fundamental understanding, applied research and demonstrating the success by fabricating large-area commercial ready cells with much higher efficiency than the traditional Si cells. By developing and integrating multiple efficiency enhancement features, namely low-cost high sheet resistance homogeneous emitter, optimized surface passivation, optimized rear reflector, back line contacts, and improved screen-
printing with narrow grid lines, 20.8% efficient screen-printed PERC (passivated emitter and rear
cell) solar cells were achieved on commercial grade 239 cm2 p-type Cz silicon wafers.
Advisors/Committee Members: Rohatgi, Ajeet (advisor), Melkote, Shreyes N. (committee member), Chang, Gee-Kung (committee member), Gaylord, Thomas (committee member), Begovic, Miroslav (committee member).
Subjects/Keywords: Silicon solar cells; Ion implantation; Selective emitter; Passivation; Passivated emitter and rear cell; Rear reflector; Light induced degradation; Local rear contacts; Fine line printing; Screen printing
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MLA ·
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Manager
APA (6th Edition):
Lai, J. (2014). Development of low-cost high-efficiency commercial-ready advanced silicon solar cells. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/52234
Chicago Manual of Style (16th Edition):
Lai, Jiun-Hong. “Development of low-cost high-efficiency commercial-ready advanced silicon solar cells.” 2014. Doctoral Dissertation, Georgia Tech. Accessed April 14, 2021.
http://hdl.handle.net/1853/52234.
MLA Handbook (7th Edition):
Lai, Jiun-Hong. “Development of low-cost high-efficiency commercial-ready advanced silicon solar cells.” 2014. Web. 14 Apr 2021.
Vancouver:
Lai J. Development of low-cost high-efficiency commercial-ready advanced silicon solar cells. [Internet] [Doctoral dissertation]. Georgia Tech; 2014. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/1853/52234.
Council of Science Editors:
Lai J. Development of low-cost high-efficiency commercial-ready advanced silicon solar cells. [Doctoral Dissertation]. Georgia Tech; 2014. Available from: http://hdl.handle.net/1853/52234
Brno University of Technology
30.
Křístek, Tomáš.
Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis.
Degree: 2019, Brno University of Technology
URL: http://hdl.handle.net/11012/59782
► Technological development, broad availability and ubiquity of mobile phones made it possible in recent years to start a progression leading to the integration of mobile…
(more)
▼ Technological development, broad availability and ubiquity of mobile phones made it possible in recent years to start a progression leading to the integration of mobile phones into biomedical analytical methods, the development of telemedicine and mobile personal health applications. This paper deals with the literature review of the mobile phone usage in biomedicine, with emphasis on the methods of optical analysis and technical solutions using 3D
printing technology. It provides a draft and practical implementation of salivary cortiol level measuring system using a mobile phone, ELISA analytical methods, 3D
printing technology and a set of custom Matlab functions connected with Matlab Mobile application and Mathworks Cloud. Also provides critical evaluation of results and method possibilities, and presents set of proposals and findings for quality of measurment optimization.
Advisors/Committee Members: Čmiel, Vratislav (advisor), Sekora, Jiří (referee).
Subjects/Keywords: Mobilní telefon; biomedicína; 3D tisk; Matlab Mobile; kortizol; ELISA; Cell phone; biomedicine; 3D printing; Matlab Mobile; cortisol; ELISA
Record Details
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Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Křístek, T. (2019). Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/59782
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Křístek, Tomáš. “Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis.” 2019. Thesis, Brno University of Technology. Accessed April 14, 2021.
http://hdl.handle.net/11012/59782.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Křístek, Tomáš. “Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis.” 2019. Web. 14 Apr 2021.
Vancouver:
Křístek T. Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Apr 14].
Available from: http://hdl.handle.net/11012/59782.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Křístek T. Využití mobilního telefonu k analýze tekutých vzorků: Use of cell phone in liquids analysis. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/59782
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
