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Massey University
1.
Hall, Alistair John.
Steady size distributions in cell populations.
Degree: Doctor Philosophy, Mathematics, 1991, Massey University
URL: http://hdl.handle.net/10179/1730 
► In any population of cells, individual cells grow for some period of time and then divide into two or more parts, called daughters. To describe…
(more)
▼ In any population of cells, individual cells grow for some period of time and then divide into two or more parts, called daughters. To describe this process mathematically, we need to specify functions describing the growth rate, size at division, and proportions into which each cell divides. In this thesis, it is assumed that the growth rate of a cell can be determined precisely from its size, but that both its size at division and the proportions into which it divides may be described stochastically, by probability density functions whose parameters are dependent on cell size and age (or birth-size). Special cases are also considered where all cells with the same birth-size divide at the same size, or where all cells divide exactly in half. We consider a population of cells growing and dividing steadily, such that the total cell population is increasing, but the proportion of cells in any size class remains constant. In Chapter 1, equations are derived which need to be solved in order to deduce the shape of the steady size distribution (or steady size/age or size/birth-size distributions) from any given growth rate and probability distributions describing the division rate and division proportions. In the general case, a Fredholm-type integral equation is obtained, but if the probability of cell division depends on cell size only (i.e. not age or birth-size), and all cells divide into equal-sized daughters, then we obtain a functional differential equation. In two special cases, the resulting equations simplify considerably, and it is these cases which are explored further in this thesis. The first is where the probability of a cell dividing in any instant of time is a constant, independent of cell age or size. In Chapter 2, the functional differential equation resulting when cells divide into equal-sized daughters is solved for the special case where the growth rate is constant, and in an appendix the case where the growth rate is described by a power law is dealt with. The second case which simplifies is where the time-independent part of the growth rate of a cell is proportional to cell size. This case is particularly important, as it is a good first-order approximation to the real cell growth rate in some structured tissues, and in some bacteria. The special case in which this leads to a functional differential equation is discussed in Chapter 3, and the integral equation arising in the general case is dealt with in Chapter 4. Finally, the conditions under which the integral operator in Chapter 4 will be both square-integrable and non-factorable are discussed in Chapter 5. It is shown that if these conditions are satisfied then a unique, stable, steady size distribution will exist.
Subjects/Keywords: Cell growth
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APA (6th Edition):
Hall, A. J. (1991). Steady size distributions in cell populations. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/1730
Chicago Manual of Style (16th Edition):
Hall, Alistair John. “Steady size distributions in cell populations.” 1991. Doctoral Dissertation, Massey University. Accessed April 11, 2021.
http://hdl.handle.net/10179/1730.
MLA Handbook (7th Edition):
Hall, Alistair John. “Steady size distributions in cell populations.” 1991. Web. 11 Apr 2021.
Vancouver:
Hall AJ. Steady size distributions in cell populations. [Internet] [Doctoral dissertation]. Massey University; 1991. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10179/1730.
Council of Science Editors:
Hall AJ. Steady size distributions in cell populations. [Doctoral Dissertation]. Massey University; 1991. Available from: http://hdl.handle.net/10179/1730
Rutgers University
2.
Brownstein, Steven Harris.
The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken.
Degree: MS, Biology, 2014, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/43492/
► Heparan sulfate proteoglycans (HSPGs) have been shown to interact with morphogens of many signaling pathway. During Drosophila oogenesis, the major contributors to tissue patterning are…
(more)
▼ Heparan sulfate proteoglycans (HSPGs) have been shown to interact with morphogens of many signaling pathway. During Drosophila oogenesis, the major contributors to tissue patterning are the bone morphogenic protein (BMP) and epidermal
growth factor receptor (EGFR) signaling pathways. It was previously shown that BMP signaling is regulated by the HSPG, dally (division abnormally delayed), in the wing, and also in patterning of the follicle cells (FCs). The EGFR pathway is responsible for axis determination as well as follicle
cell pattering. Using genetic perturbation, we demonstrate that Dally regulates the distribution of EGFR signaling through the restriction of the TGFα-like ligand Gurken (GRK). When dally is perturbed by uniform overexpression or depletion in the FCs, the GRK gradient is either narrowed, or widened, respectively. In these backgrounds, changes in EGFR activation, measured by dpERK, are consistent with the shapes of GRK patterning. These effects on EGFR activation lead to corresponding results on follicle
cell pattering where a decrease in midline clearing of BR in overexpression of dally, corresponding to a reduced gap between the dorsal appendages (DAs). Expressing a mutant form of Dally, lacking an anchor to the membrane, perturbed the GRK gradient, leading to tissue patterning and eggshell morphology defects. Based upon these results, we propose that Dally is required for the formation of the GRK gradient for optimal EGFR signaling activation.
Advisors/Committee Members: Yakoby, Nir (chair), Shain, Daniel (internal member), Nam, Jongmin (internal member).
Subjects/Keywords: Epidermal growth factor; Cell aggregation
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APA (6th Edition):
Brownstein, S. H. (2014). The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/43492/
Chicago Manual of Style (16th Edition):
Brownstein, Steven Harris. “The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken.” 2014. Masters Thesis, Rutgers University. Accessed April 11, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/43492/.
MLA Handbook (7th Edition):
Brownstein, Steven Harris. “The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken.” 2014. Web. 11 Apr 2021.
Vancouver:
Brownstein SH. The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2021 Apr 11].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/43492/.
Council of Science Editors:
Brownstein SH. The glypican Dally shapes follicle cell patterning by regulating the epidermal growth factor receptor ligand Gurken. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/43492/
University of Rochester
3.
Gildner, Candace D. (1979 - ).
Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin.
Degree: PhD, 2011, University of Rochester
URL: http://hdl.handle.net/1802/14776
► Extracellular matrix (ECM) fibronectin directs many cellular activities that are critical for tissue development and regeneration, including cell growth, migration, and contractility. The factors that…
(more)
▼ Extracellular matrix (ECM) fibronectin directs many
cellular activities that are
critical for tissue development and
regeneration, including cell growth, migration, and
contractility.
The factors that regulate the deposition of fibronectin into the
ECM and
govern how cells respond to ECM fibronectin during tissue
repair processes are
unknown. The deposition of other ECM
proteins, including vitronectin and fibrin, into
a provisional ECM
in response to tissue injury may be one mechanism by which
cellular responses to ECM fibronectin are controlled. Vitronectin
has been shown to
modulate the deposition of ECM fibronectin,
suggesting that the presence of
vitronectin during tissue
regenerative processes regulates cellular responses to ECM
fibronectin. The goal of the studies performed in this thesis was
to examine the
effects of vitronectin on the functional properties
of ECM fibronectin and the
deposition of fibronectin into the
ECM.
The data presented in this thesis show that
vitronectin-adherent fibronectin-null
cells produce a fibrillar
detergent-insoluble fibronectin matrix when cultured in a
defined
serum-free media. The fibronectin matrix produced by
vitronectin-adherent
cells was 10-fold less effective at
stimulating cell growth than the fibronectin matrix
produced by
collagen-adherent cells (EC50 = 3.7 ± 1.2 nM and 41.1 ± 1.17 nM
for
collagen- and vitronectin-adherent cells, respectively). In
contrast, vitronectin- and
collagen-adherent cells exhibited a
similar 1.8-fold-increase in cell number when
treated with a
fibronectin fusion protein, GST/III-1H,8-10, demonstrating that
reduced
cell proliferation is not a general property of
vitronectin-adherent cells. Fibronectin-null
cells adhered to a
vitronectin fragment containing the cell binding domain of
vitronectin also exhibited a decreased growth response to
fibronectin, suggesting
that interactions of vitronectin with
integrins on the cell surface play a role in
mediating cellular
responses to ECM fibronectin. However, the reduced growth
response
to fibronectin observed for vitronectin-adherent cells was not a
general
property of [alpha]v[beta]3 integrin-binding substrates,
as cell adhesion to either gelatin or
fibrinogen resulted in a
fibronectin-mediated growth response similar to collagen-adherent
cells. Nor was the less efficient fibronectin growth response of
vitronectin-adherent
cells due to a decreased deposition of
detergent-insoluble fibronectin as
addition of a fibronectin
fragment that enhances the deposition of detergent-insoluble
fibronectin did not enhance the fibronectin growth response of
cells adherent to
vitronectin. Taken together, these data suggest
that fibronectin matrices formed by
cells can differ in their
ability to modulate cell growth and that the presence of
vitronectin regulates the growth-promoting properties of ECM
fibronectin.
Strain-induced conformational changes in the III-1
module of ECM fibronectin
may facilitate the exposure of the
cryptic heparin binding domain in III-1 and may be
one…
Subjects/Keywords: Extracellular matrix; Fibronectin; Vitronectin; Cell growth/proliferation
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APA (6th Edition):
Gildner, C. D. (. -. ). (2011). Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin. (Doctoral Dissertation). University of Rochester. Retrieved from http://hdl.handle.net/1802/14776
Chicago Manual of Style (16th Edition):
Gildner, Candace D (1979 - ). “Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin.” 2011. Doctoral Dissertation, University of Rochester. Accessed April 11, 2021.
http://hdl.handle.net/1802/14776.
MLA Handbook (7th Edition):
Gildner, Candace D (1979 - ). “Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin.” 2011. Web. 11 Apr 2021.
Vancouver:
Gildner CD(-). Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin. [Internet] [Doctoral dissertation]. University of Rochester; 2011. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/1802/14776.
Council of Science Editors:
Gildner CD(-). Effect of vitronectin on the deposition, conformation,
and physiologic properties of extracellular matrix
fibronectin. [Doctoral Dissertation]. University of Rochester; 2011. Available from: http://hdl.handle.net/1802/14776
Vanderbilt University
4.
Bohnert, Kenneth Adam.
Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation.
Degree: PhD, Cell and Developmental Biology, 2013, Vanderbilt University
URL: http://hdl.handle.net/1803/13123
► In many organisms, a cytokinetic ring directs daughter cell separation following mitosis. While conserved molecular participants in this process have been defined, the signaling events…
(more)
▼ In many organisms, a cytokinetic ring directs daughter
cell separation following mitosis. While conserved molecular participants in this process have been defined, the signaling events controlling cytokinetic ring function remain obscure. Using a genetically-tractable fission yeast, Schizosaccharomyces pombe, I have investigated mechanisms involved in such signaling, with a particular interest in kinase and phosphatase networks. Through identification of a new subunit of the S. pombe chromosomal passenger complex, I have found that Aurora B kinase influences cytokinesis by mediating Cdc14-family phosphatase accumulation at the cytokinetic ring. In addition, I have discovered that Sid2, a kinase of the S. pombe septation initiation network, phosphorylates cytokinetic formin Cdc12 to reverse formin multimerization and allow cytokinetic ring maintenance. My studies also indicate that cytokinesis impacts
cell cycle-dependent polarized
growth, and that phosphosignaling at the cytokinetic ring ensures robust
growth following
cell division. These studies advance our understanding of molecular cues regulating cytokinesis, and broaden knowledge concerning the consequences of this control.
Advisors/Committee Members: Kathleen L. Gould (committee member), Stephen R. Hann (committee member), Matthew J. Tyska (committee member), Ellen H. Fanning (committee member), Susan R. Wente (Committee Chair).
Subjects/Keywords: cytokinesis; cell growth; phosphorylation; formin; kinase; morphogenesis
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APA (6th Edition):
Bohnert, K. A. (2013). Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13123
Chicago Manual of Style (16th Edition):
Bohnert, Kenneth Adam. “Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation.” 2013. Doctoral Dissertation, Vanderbilt University. Accessed April 11, 2021.
http://hdl.handle.net/1803/13123.
MLA Handbook (7th Edition):
Bohnert, Kenneth Adam. “Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation.” 2013. Web. 11 Apr 2021.
Vancouver:
Bohnert KA. Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation. [Internet] [Doctoral dissertation]. Vanderbilt University; 2013. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/1803/13123.
Council of Science Editors:
Bohnert KA. Divide and Prosper: Molecular Mechanisms and Consequences of Cytokinetic Ring Regulation. [Doctoral Dissertation]. Vanderbilt University; 2013. Available from: http://hdl.handle.net/1803/13123
Texas A&M University
5.
Whitely Jr, Michael Edward.
Development of PolyHIPE Grafts for Guided Bone Regeneration.
Degree: PhD, Biomedical Engineering, 2018, Texas A&M University
URL: http://hdl.handle.net/1969.1/173994
► A pressing need exists to develop an improved bone replacement to treat the millions of non-union fractures that occur each year as a result of…
(more)
▼ A pressing need exists to develop an improved bone replacement to treat the millions of non-union fractures that occur each year as a result of severe trauma, tumor resection, spinal fusions, and joint replacements. Current bone grafts are often hindered by a lack of biodegradability, porosity or innate ability to promote regeneration. This work employs tissue engineering to design a novel bone replacement that combines the regenerative potential of autologous tissue with the tunability of synthetic grafts. This is accomplished by engineering a biodegradable scaffold with physical and mechanical properties emulating those of cancellous bone and combining this scaffold with technologies that allow for the controlled delivery of stem cells and osteoinductive factors.
In this work, polymerized high internal phase emulsions (polyHIPEs) were developed as an injectable, high porosity bone graft. Thiol-methacrylate polyHIPEs were investigated to increase resistance to oxygen inhibition and improve scaffold function under clinically relevant conditions. Methods were established to modulate and characterize scaffold porosity, cure rate, compressive properties, and degradation rates. Furthermore,
cell-laden poly(ethylene glycol)-dithiothreitol hydrogels were developed to improve loading and distribution of human mesenchymal stem cells (hMSCs) within 3D printed polyHIPEs. This approach allowed for increased
cell retention and supported critical markers of osteoblastic differentiation. Finally, to confer additional osteoinductive character, porous microspheres with tunable release kinetics and requisite compressive properties were fabricated using a solvent-free, in-line loading approach. Bioactivity retention of encapsulated bone morphogenetic protein-2, along with its ability to promote osteoblastic differentiation of hMSCs, was explored.
Overall, these studies highlight the strong potential of polyHIPE scaffolds to serve as an improved bone replacement with the ability to actively guide bone regeneration. Key technologies have been developed that allow for fabrication of a bone graft with improved function in a clinically relevant setting, efficient seeding with mesenchymal stem cells, and targeted delivery of osteoinductive factors. Fundamentally, this work will be an invaluable tool in identifying and evaluating critical design requirements for future bone graft design.
Advisors/Committee Members: Cosgriff-Hernandez, Elizabeth (advisor), Gaharwar, Akhilesh (advisor), Kaunas, Roland (committee member), Saunders, William (committee member).
Subjects/Keywords: PolyHIPE; Bone Graft; Stem Cell; Growth Factor
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APA (6th Edition):
Whitely Jr, M. E. (2018). Development of PolyHIPE Grafts for Guided Bone Regeneration. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173994
Chicago Manual of Style (16th Edition):
Whitely Jr, Michael Edward. “Development of PolyHIPE Grafts for Guided Bone Regeneration.” 2018. Doctoral Dissertation, Texas A&M University. Accessed April 11, 2021.
http://hdl.handle.net/1969.1/173994.
MLA Handbook (7th Edition):
Whitely Jr, Michael Edward. “Development of PolyHIPE Grafts for Guided Bone Regeneration.” 2018. Web. 11 Apr 2021.
Vancouver:
Whitely Jr ME. Development of PolyHIPE Grafts for Guided Bone Regeneration. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/1969.1/173994.
Council of Science Editors:
Whitely Jr ME. Development of PolyHIPE Grafts for Guided Bone Regeneration. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/173994
Loughborough University
6.
Worrallo, Matthew J.
Immobilised growth factors for scalable cell therapy manufacturing platforms.
Degree: PhD, 2018, Loughborough University
URL: http://hdl.handle.net/2134/27912
► Regenerative medicine has the potential to establish or restore normal function in defective tissues and organs. The realisation of such therapies is restricted due to…
(more)
▼ Regenerative medicine has the potential to establish or restore normal function in defective tissues and organs. The realisation of such therapies is restricted due to costs, lack of scalability and inefficient manufacturing process controls. A major contributor to cost is the use of expensive growth factors supplemented into media at high concentrations. In vivo, growth factors exist in soluble, immobilised and transmembrane forms, expressed in a spatiotemporal fashion within the stem cell niche. In comparison to soluble equivalents, immobilised growth factors exhibit increased potency, distinct functional activities, improved cell phenotypic control and act in synergy with other soluble and immobilised ligands. To date, most research into immobilised growth factors has been restricted to planar cell culture surfaces such as tissue culture plastics which have limited scalability. To address the scalability limitations, a novel growth factor immobilisation technology was developed using magnetic microparticles which can be scaled with respect to surface area to volume ratio in standard stirred tank bioreactors. Three clinically relevant growth factors, SCF, TPO and GM-CSF were immobilised and were shown to remain functionally active where surface concentration could be manipulated in a number of ways. Through a series of experiments, it was demonstrated that immobilised growth factors exhibited ~10-fold increase in potency compared with soluble equivalents and remain stable for up to 192 hours following recycling during multiple media passages. Immobilised growth factors were able to expand more cells over a longer period of time after transient exposure and finally, the immobilisation technique was successfully applied to the expansion of umbilical cord derived haematopoietic stem cells using immobilised SCF. The immobilisation method described here has the potential to significantly reduce media costs in large scale cell manufacturing processes.
Subjects/Keywords: 615.5; Growth factors; Cell therapy; Scalable; Immobilised
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APA (6th Edition):
Worrallo, M. J. (2018). Immobilised growth factors for scalable cell therapy manufacturing platforms. (Doctoral Dissertation). Loughborough University. Retrieved from http://hdl.handle.net/2134/27912
Chicago Manual of Style (16th Edition):
Worrallo, Matthew J. “Immobilised growth factors for scalable cell therapy manufacturing platforms.” 2018. Doctoral Dissertation, Loughborough University. Accessed April 11, 2021.
http://hdl.handle.net/2134/27912.
MLA Handbook (7th Edition):
Worrallo, Matthew J. “Immobilised growth factors for scalable cell therapy manufacturing platforms.” 2018. Web. 11 Apr 2021.
Vancouver:
Worrallo MJ. Immobilised growth factors for scalable cell therapy manufacturing platforms. [Internet] [Doctoral dissertation]. Loughborough University; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/2134/27912.
Council of Science Editors:
Worrallo MJ. Immobilised growth factors for scalable cell therapy manufacturing platforms. [Doctoral Dissertation]. Loughborough University; 2018. Available from: http://hdl.handle.net/2134/27912
Hong Kong University of Science and Technology
7.
Chen, Li LIFS.
Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.
Degree: 2017, Hong Kong University of Science and Technology
URL: http://repository.ust.hk/ir/Record/1783.1-105622
;
https://doi.org/10.14711/thesis-991012564169103412
;
http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html
► Herein I describe a genetic study that employed a temperature-sensitive allele encoding the Golgi glycosyltransferase sorting receptor VPS74. Through characterizing genes that act as dosage…
(more)
▼ Herein I describe a genetic study that employed a temperature-sensitive allele encoding the Golgi glycosyltransferase sorting receptor VPS74. Through characterizing genes that act as dosage suppressors of the temperature-sensitivity of vps74-1 cells several genes that function in protein trafficking between the ER and Golgi were identified. In addition to trafficking genes, unexpectedly, genes involved in cell cycle regulation were also identified. In this thesis I focused on establishing the functional significance to vps74-1 cells of a subset of the cell cycle genes involved in the spindle assembly checkpoint. Through a succession of genetic studies, pharmacological and cell biological approaches I establish that vps74-1 cells are defective in the processing of glycosylphosphatidylinositol anchored proteins. The defect in the processing of glycosylphosphatidylinositol anchored proteins in vps74-1 cells is a consequence of mutations in two glycosylphosphatidylinositol anchored protein remodelases termed Ted1p and Dcr2p. I have determined defects in the remodeling of glycosylphosphatidylinositol anchored proteins - specifically the removal of phosphoethanolamine from mannose 2 of yeast glycosylphosphatidylinositol anchored proteins results in activation of the spindle assembly checkpoint.
Subjects/Keywords: Yeast
; Genetics
; Growth
; Proteins
; Cell receptors
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APA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chen, L. L. (2017). Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Chen, Li LIFS. “Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.” 2017. Thesis, Hong Kong University of Science and Technology. Accessed April 11, 2021.
http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Chen, Li LIFS. “Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells.” 2017. Web. 11 Apr 2021.
Vancouver:
Chen LL. Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2017. [cited 2021 Apr 11].
Available from: http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Chen LL. Mutations in TED1 and DCR2, two glycosylphosphatidylinositol anchored protein remodelases, activate the spindle assembly checkpoint in budding yeast cells. [Thesis]. Hong Kong University of Science and Technology; 2017. Available from: http://repository.ust.hk/ir/Record/1783.1-105622 ; https://doi.org/10.14711/thesis-991012564169103412 ; http://repository.ust.hk/ir/bitstream/1783.1-105622/1/th_redirect.html
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
8.
Elmaarouf, Rhizlane.
Modeling and control of psoriasis.
Degree: MS, Mathematics and Computer Science, 2016, Texas Woman's University
URL: http://hdl.handle.net/11274/8296
► In this paper a mathematical model of psoriasis is created and investigated. The model is described by a nonlinear control system of three differential equations…
(more)
▼ In this paper a mathematical model of psoriasis is created and investigated. The model is described by a nonlinear control system of three differential equations involving the concentration of Dendritic Cells (Tissues Macrophages), T-Lymphocytes and Keratinocytes. Analytical and numerical studies techniques will be discussed. An optimal control problem of minimizing the release of Keratinocytes to standardize the
growth of the Dendritic Cells is stated and solved using Pontryagin Maximum Principle. Optimal solution and optimal controls are presented at different values of the model.
Advisors/Committee Members: Grigorieva, Ellina (Committee Chair), Edwards, Don (committee member).
Subjects/Keywords: Mathematics; Pontryagin Maximum Principle; Dendritic cell growth
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APA (6th Edition):
Elmaarouf, R. (2016). Modeling and control of psoriasis. (Masters Thesis). Texas Woman's University. Retrieved from http://hdl.handle.net/11274/8296
Chicago Manual of Style (16th Edition):
Elmaarouf, Rhizlane. “Modeling and control of psoriasis.” 2016. Masters Thesis, Texas Woman's University. Accessed April 11, 2021.
http://hdl.handle.net/11274/8296.
MLA Handbook (7th Edition):
Elmaarouf, Rhizlane. “Modeling and control of psoriasis.” 2016. Web. 11 Apr 2021.
Vancouver:
Elmaarouf R. Modeling and control of psoriasis. [Internet] [Masters thesis]. Texas Woman's University; 2016. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/11274/8296.
Council of Science Editors:
Elmaarouf R. Modeling and control of psoriasis. [Masters Thesis]. Texas Woman's University; 2016. Available from: http://hdl.handle.net/11274/8296
9.
Hernandez-Sanchez, Arianna Michelle.
Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810).
Degree: 2017, University of Melbourne
URL: http://hdl.handle.net/11343/194830
► Arabinogalactan-proteins (AGPs) are proteoglycans found in all plants and are involved in plant growth and development. AGPs consist of a protein core generally rich in…
(more)
▼ Arabinogalactan-proteins (AGPs) are proteoglycans found in all plants and are involved in plant growth and development. AGPs consist of a protein core generally rich in hydroxyproline (Hyp) that is O-glycosylated with type II arabino-β-(1,3;1,6)-galactans (AGs) and some oligo-arabinosides. For decades little had been known about the enzymes involved in the glycosylation of the AGP protein core. In 2008, Qu et al. sugested that the enzymes responsible of the synthesis of AGs were found in the CAZy GT 31 family. In the present study the role of the two putative galactosyltransferases (GalT), At1g74800 (AtGALT5) and At1g77810 (AtGALT9), in the synthesis of AGPs was investigated through genetic and biochemical approaches. The aims of this thesis were: 1) Characterise the expression patterns of AtGALT5 and AtGALT9, 2) Investigate the consequence of the loss-of-function and over-expression of AtGALT5 and AtGALT9 in Arabidopsis and 3) Characterise the in vitro enzyme activity of AtGALT5 and AtGALT9.
Gene expression analysis carried out using in silico expression data, RT-PCR and GUS reporter promoter fusions indicated that AtGALT5 and AtGALT9 expression is developmentally regulated and located to all organs but in specific cell types. AtGALT5 and AtGALT9 were strongly expressed in mature pollen grains. atgalt5 and atgalt9 mutants had shorter stems and roots compared to wild type and displayed salt root growth hypersensitivity. Lower amounts of AGPs were present in the flowers of atgalt5 and atgalt9 plants suggesting a possible role of AtGALT5 and AtGALT9 in AGP glycosylation. AtGALT5 and AtGALT9 were localised to the Golgi apparatus (GA), an organelle where the synthesis of type II AGs (β-(1,3;1,6)-galactans) takes place. Interestingly, over-expression of AtGALT5-VENUS in Arabidopsis led to alterations in cell size and higher levels of AGP epitopes recognised by the LM2, MAC204, JIM16, JIM17, and CCRCM7 AGP antibodies. This suggested an impairment in cell expansion likely due to abnormal glycosylation of cell surface AGPs in AtGALT5-VENUS overexpressing plants. In vitro GalT assays using β-Gal-NBD as an acceptor indicated that AtGALT9 transiently expressed in Nicotiana benthamiana has β-(1,3)-GalT activity. In conclusion, AtGALT5 is a GA-localised putative GalT involved in the synthesis of type II AGs of AGPs whose function is important in cell expansion. AtGALT9 is a β-(1,3)- GalT likely to be involved in the elongation of the β-(1,3)-D-galactan backbone of AGPs. Both AtGALT5 and AtGALT9 being required for normal root and stem growth.
Subjects/Keywords: Arabinogalactan-proteins; galactosyltransferases; cell expansion; root growth
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APA (6th Edition):
Hernandez-Sanchez, A. M. (2017). Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810). (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/194830
Chicago Manual of Style (16th Edition):
Hernandez-Sanchez, Arianna Michelle. “Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810).” 2017. Doctoral Dissertation, University of Melbourne. Accessed April 11, 2021.
http://hdl.handle.net/11343/194830.
MLA Handbook (7th Edition):
Hernandez-Sanchez, Arianna Michelle. “Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810).” 2017. Web. 11 Apr 2021.
Vancouver:
Hernandez-Sanchez AM. Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810). [Internet] [Doctoral dissertation]. University of Melbourne; 2017. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/11343/194830.
Council of Science Editors:
Hernandez-Sanchez AM. Investigating the function of two putative Arabidopsis thaliana AGP galactosyltransferases: AtGALT5 (At1g74800) and AtGALT9 (At1g77810). [Doctoral Dissertation]. University of Melbourne; 2017. Available from: http://hdl.handle.net/11343/194830
University of Georgia
10.
Johnson, Lisa Klima.
Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/29860
► Fruit size in apple is determined through the combination of cell production and expansion. Cell cycle genes identified in apple include two that may negatively…
(more)
▼ Fruit size in apple is determined through the combination of cell production and expansion. Cell cycle genes identified in apple include two that may negatively regulate cell production. These genes are cyclin dependent kinase inhibitors,
referred to as KRPs (Kip-Related Proteins). Characterization of these genes is needed to determine how they influence fruit growth and size. Two approaches included identification of polymorphisms within the coding region of these KRPs in a population of
Malus × domestica varying in fruit size, and transformation of Arabidopsis thaliana with MdKRP4 and MdKRP5. One polymorphism identified in MdKRP4 resulted in an amino acid substitution that correlated with small fruit size. Preliminary phenotypic
observations of the transformants indicate smaller, serrated leaves and altered floral morphology, similar to plants overexpressing A. thaliana KRP2. These results indicate the KRPs have an important role in cell cycle regulation, potentially impacting
cell production and fruit growth in apple.
Subjects/Keywords: Malus × domestica; cell cycle; KRP; fruit growth
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APA (6th Edition):
Johnson, L. K. (2014). Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/29860
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Johnson, Lisa Klima. “Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5.” 2014. Thesis, University of Georgia. Accessed April 11, 2021.
http://hdl.handle.net/10724/29860.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Johnson, Lisa Klima. “Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5.” 2014. Web. 11 Apr 2021.
Vancouver:
Johnson LK. Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10724/29860.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Johnson LK. Identification of single nucleotide polymorphisms and the functional characterization of two apple Kip-related proteins, MdKRP4 and MdKRP5. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/29860
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Toronto
11.
Samsonoff, Nathan George.
Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production.
Degree: 2013, University of Toronto
URL: http://hdl.handle.net/1807/42922
► Photosynthetic biofilms have much higher cell density than suspended cultures and when grown in a stacked waveguide configuration, can have orders of magnitude higher areal…
(more)
▼ Photosynthetic biofilms have much higher cell density than suspended cultures and when grown in a stacked waveguide configuration, can have orders of magnitude higher areal productivity. Evanescent and plasmonic growth of biofilm cultures have been demonstrated, solving issues with light penetration impeding growth, but thus far the technology has been limited to biofuel production applications.
In this thesis, plasmonically excited cyanobacterial biofilms are used to produce electrical power in a photosynthetic-plasmonic-voltaic device. This approach uses red lasers to deliver light to cells via an optical waveguide through the generation of surface plasmons at the interface between a metal and dielectric, in this case a glass-gold-air interface. This gold film serves a dual purpose as a current collector for electrons generated at the cell surface. Experiments presented here demonstrate positive power output light response under both direct light and plasmonic excitation and produced equivalent power output of 6 uW/m2 under similar light power intensities.
MAST
Advisors/Committee Members: Sinton, David, Mechanical and Industrial Engineering.
Subjects/Keywords: plasmonic cell growth; biophotovoltaics; evanescent cell growth; photosynthetic microbial fuel cells; microbial electrochemical technologies; 0548
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APA (6th Edition):
Samsonoff, N. G. (2013). Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/42922
Chicago Manual of Style (16th Edition):
Samsonoff, Nathan George. “Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production.” 2013. Masters Thesis, University of Toronto. Accessed April 11, 2021.
http://hdl.handle.net/1807/42922.
MLA Handbook (7th Edition):
Samsonoff, Nathan George. “Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production.” 2013. Web. 11 Apr 2021.
Vancouver:
Samsonoff NG. Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production. [Internet] [Masters thesis]. University of Toronto; 2013. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/1807/42922.
Council of Science Editors:
Samsonoff NG. Photosynthetic-plasmonic-voltaics: Plasmonically Excited Biofilms for Electricity Production. [Masters Thesis]. University of Toronto; 2013. Available from: http://hdl.handle.net/1807/42922
Columbia University
12.
Moore, Emily.
The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation.
Degree: 2018, Columbia University
URL: https://doi.org/10.7916/D8JW9RVP
► Primary cilia are sensory organelles that facilitate early skeletal development, as well as maintenance and adaptation of bone later in life. These solitary, immotile organelles…
(more)
▼ Primary cilia are sensory organelles that facilitate early skeletal development, as well as maintenance and adaptation of bone later in life. These solitary, immotile organelles are known to be involved in cell differentiation, proliferation, and mechanotransduction, a process by which cells sense and covert external physical stimuli into intracellular biochemical signals. Bone is a metabolically active tissue that continuously recruits osteogenic precursors and relies on osteocytes, the sensory cells of bone, to coordinate skeletal maintenance. Overall bone quality is dependent on the integrity of the initial structure formed, as well as this organ’s ability to adapt to physical loads. Proper differentiation and controlled proliferation of osteogenic progenitors are critical to the initial formation of the skeleton, while osteocyte mechanotransduction is essential for adaptation of developed bone. These phenomena rely on primary cilia, but little is known about the origin of osteogenic precursors and the ciliary mechanisms that promote osteogenesis.
In this thesis, we first characterize an osteochondroprogenitor (OCP) population that rapidly and extensively populates skeletal tissues during juvenile skeletal development (Chapter 2). We also demonstrate that the primary cilium is critical for these cells to differentiate and contribute to skeletogenesis. We then show this OCP population is required for adult bone adaptation and is mechanoresponsive (Chapter 3). Again, we demonstrate that primary cilia are necessary for these OCPs to sense physical stimuli and differentiate into active bone-forming cells. Finally, we identify a novel link between ciliary calcium and cAMP dynamics in the osteocyte primary cilium (Chapter 4). Specifically, we show that a calcium channel (TRPV4) and adenylyl cyclases, which produce cAMP, bind calcium to mediate calcium entry and cAMP production, respectively, and these phenomena are critical to fluid flow-induced osteogenesis. Collectively, our results demonstrate that an easily extracted progenitor population is pre-programmed towards an osteogenic fate and extensively contributes to bone generation through primary cilium-mediated mechanisms at multiple stages of life. Furthermore, we identified ciliary proteins that are potentially unique to the osteocyte and can be manipulated to encourage osteogenesis by tuning calcium/ cAMP dynamics. For these reasons, we propose that this OCP population and their primary cilia, as well as osteocyte ciliary proteins that coordinate calcium/ cAMP dynamics, are attractive therapeutic targets to encourage bone regeneration.
Subjects/Keywords: Biomedical engineering; Bones – Growth; Stem cells; Cell organelles; Skeleton – Growth
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APA (6th Edition):
Moore, E. (2018). The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8JW9RVP
Chicago Manual of Style (16th Edition):
Moore, Emily. “The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation.” 2018. Doctoral Dissertation, Columbia University. Accessed April 11, 2021.
https://doi.org/10.7916/D8JW9RVP.
MLA Handbook (7th Edition):
Moore, Emily. “The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation.” 2018. Web. 11 Apr 2021.
Vancouver:
Moore E. The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation. [Internet] [Doctoral dissertation]. Columbia University; 2018. [cited 2021 Apr 11].
Available from: https://doi.org/10.7916/D8JW9RVP.
Council of Science Editors:
Moore E. The primary cilium encourages osteogenic behavior in periosteal osteochondroprogenitors and osteocytes during juvenile skeletal development and adult bone adaptation. [Doctoral Dissertation]. Columbia University; 2018. Available from: https://doi.org/10.7916/D8JW9RVP
University of Illinois – Chicago
13.
Elangovan, Indira Maheshwari.
Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications.
Degree: 2017, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/21907
► There are limited therapeutic strategies to mitigate mutant KRAS activity in lung cancer which is the leading cause of cancer deaths in the US. Constitutively…
(more)
▼ There are limited therapeutic strategies to mitigate mutant KRAS activity in lung cancer which is the leading cause of cancer deaths in the US. Constitutively active mutant KRAS in alveolar epithelium promotes lung tumorigenesis. My study focuses on investigating the role of AP-1 transcription factors, FOSL1 and JUN, in human lung adenocarcinoma present downstream of the RAS-ERK1/2 signaling pathway and to further identify if they are the crucial downstream effectors of mutant KRAS and mediate lung tumorigenesis in vivo. In vivo findings in my study suggest that using either Fosl1 or Jun deletion or a combination of both increased survival of mice with activated mutant Kras in lungs, demonstrating its role in promoting mutant KRAS-induced lung cancer in vivo. Mechanistic studies done on mutant KRAS Human Lung adenocarcinoma cells (HLAC) suggest that both FOSL1 and JUN regulates KRAS mutant HLAC
cell proliferation by inducing the expression of amphiregulin (AREG), a
growth factor that activates the EGFR signaling. In addition, FOSL1 promotes KRAS mutant HLAC
cell survival by regulating the expression of antioxidant genes (such as NQO1 and HMOX1) and apoptotic genes (such as BCL2 and BCLXL) but JUN does not alter their expression levels. Furthermore, in non-mutant KRAS HLAC cells FOSL1 regulates various chemokines which are known regulators of tumor survival. These results enable us to potentially target either FOSL1 or JUN individually or as a combinatorial therapy against human lung adenocarcinoma.
Advisors/Committee Members: Reddy, Sekhar (advisor), Natarajan, Viswanathan (committee member), Ackerman, Steven J. (committee member), Winn, Robert A (committee member), Tyner, Angela L (committee member), Reddy, Sekhar (chair).
Subjects/Keywords: AP-1; tumor cell growth; proliferation; survival; cyclins; growth factor; antioxidants
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APA (6th Edition):
Elangovan, I. M. (2017). Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/21907
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Elangovan, Indira Maheshwari. “Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications.” 2017. Thesis, University of Illinois – Chicago. Accessed April 11, 2021.
http://hdl.handle.net/10027/21907.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Elangovan, Indira Maheshwari. “Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications.” 2017. Web. 11 Apr 2021.
Vancouver:
Elangovan IM. Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10027/21907.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Elangovan IM. Regulation of Lung Cancer by FOSL1 and JUN Transcription Factors: Novel Insights and Implications. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/21907
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Ottawa
14.
Jing, Wenyang.
A Microfluidic Volume Sensor for Single-Cell Growth Measurements
.
Degree: 2016, University of Ottawa
URL: http://hdl.handle.net/10393/34770
► The multidisciplinary field of microfluidics has shown great promise for research at the interface of biology, chemistry, engineering, and physics. Laminar flow, versatile fabrication, and…
(more)
▼ The multidisciplinary field of microfluidics has shown great promise for research at the interface of biology, chemistry, engineering, and physics. Laminar flow, versatile fabrication, and small length scales have made microfluidics especially well-suited for single-cell characterization. In particular, the evaluation of single-cell growth rates is of fundamental interest for studying the cell cycle and the effects of environmental factors, such as drugs, on cellular growth. This work presents aspects in the development of a microfluidic cell impedance sensor for measuring the volumetric growth rate of single cells and covers its application in the investigation of a new discovery relating to multidrug resistance in S. cerevisiae. While there are many avenues for the utilization and interpretation of growth rates, this application focused on the quantitative assessment of biological fitness—an important parameter in population genetics and mathematical biology. Through a combination of growth measurements and optics, this work concludes a novel case of bet-hedging in yeast, as well as the first ever case of bet-hedging in eukaryotic multidrug resistance.
Subjects/Keywords: Microfluidics;
Cell Growth;
Impedance Cytometry;
Cell Volume Sensor;
Microfabrication;
Bet-Hedging
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APA (6th Edition):
Jing, W. (2016). A Microfluidic Volume Sensor for Single-Cell Growth Measurements
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/34770
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jing, Wenyang. “A Microfluidic Volume Sensor for Single-Cell Growth Measurements
.” 2016. Thesis, University of Ottawa. Accessed April 11, 2021.
http://hdl.handle.net/10393/34770.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jing, Wenyang. “A Microfluidic Volume Sensor for Single-Cell Growth Measurements
.” 2016. Web. 11 Apr 2021.
Vancouver:
Jing W. A Microfluidic Volume Sensor for Single-Cell Growth Measurements
. [Internet] [Thesis]. University of Ottawa; 2016. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10393/34770.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jing W. A Microfluidic Volume Sensor for Single-Cell Growth Measurements
. [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/34770
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
15.
Dash, Madhumita.
Fuit growth in apple.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/28504
► Fruit size in apple (Malus x domestica) is of great economic significance. A thorough comprehension of mechanisms that regulate fruit growth and development is essential…
(more)
▼ Fruit size in apple (Malus x domestica) is of great economic significance. A thorough comprehension of mechanisms that regulate fruit growth and development is essential to optimize fruit size. In this study, the factors affecting
shade-induced and thinning-induced alteration in fruit growth were determined. The results demonstrate that shade-induced reduction in fruit growth and thinning-induced increase in fruit growth is facilitated by coordinated changes in the expression of
carbohydrate metabolism-related genes, transcription factors associated with fruit growth, and key genes associated with cell production and expansion. The changes in the expression of these genes may regulate fruit growth by altering the key processes
of cell production and expansion. AINTEGUMENTA (ANT), an AP2 domain transcription factor, controls organ size in Arabidopsis by regulating the duration of cell production and is a candidate for fruit growth regulation in apple. Two genes homologous to
the Arabidopsis ANT, MdANT1 and MdANT2, were isolated from apple. The expression of these genes was analyzed during fruit development, in response to factors affecting fruit size, and across genotypes. The results demonstrate that the expression of these
ANTs is closely associated with cell production during fruit development. Additionally, wild-type Arabidopsis plants were transformed with Act7
Subjects/Keywords: Cell division; cell expansion; fruit development; fruit size; organ growth
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APA ·
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MLA ·
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Export
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Manager
APA (6th Edition):
Dash, M. (2014). Fuit growth in apple. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/28504
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dash, Madhumita. “Fuit growth in apple.” 2014. Thesis, University of Georgia. Accessed April 11, 2021.
http://hdl.handle.net/10724/28504.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dash, Madhumita. “Fuit growth in apple.” 2014. Web. 11 Apr 2021.
Vancouver:
Dash M. Fuit growth in apple. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10724/28504.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dash M. Fuit growth in apple. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/28504
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Loughborough University
16.
Al-Saedi, Hayder M.
Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model.
Degree: PhD, 2018, Loughborough University
URL: http://hdl.handle.net/2134/35605
► We present a theoretical framework based on an extension of Dynamical Density Functional Theory (DDFT) to describe the structure and dynamics of cells in living…
(more)
▼ We present a theoretical framework based on an extension of Dynamical Density Functional Theory (DDFT) to describe the structure and dynamics of cells in living tissues and tumours. DDFT is a microscopic statistical mechanical theory for the time evolution of the density distribution of interacting many-particle systems. The theory accounts for cell pair-interactions, different cell types, phenotypes and cell birth and death processes (including cell division), in order to provide a biophysically consistent description of processes bridging across the scales, including the description of the tissue structure down to the level of the individual cells. Analysis of the model is presented for a single species and a two-species cases, the latter describing competition between a cancerous and healthy cells. In suitable parameter regimes, model results are consistent with biological observations. Of particular note, divergent tumour growth behaviour, mirroring metastatic and benign growth characteristics, are shown to be dependent on the cell pair-interaction parameters.
Subjects/Keywords: 616.99; Dynamical Density Functional Theory; Tumour growth; Cell-cell interactions
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APA ·
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MLA ·
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Export
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Manager
APA (6th Edition):
Al-Saedi, H. M. (2018). Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model. (Doctoral Dissertation). Loughborough University. Retrieved from http://hdl.handle.net/2134/35605
Chicago Manual of Style (16th Edition):
Al-Saedi, Hayder M. “Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model.” 2018. Doctoral Dissertation, Loughborough University. Accessed April 11, 2021.
http://hdl.handle.net/2134/35605.
MLA Handbook (7th Edition):
Al-Saedi, Hayder M. “Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model.” 2018. Web. 11 Apr 2021.
Vancouver:
Al-Saedi HM. Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model. [Internet] [Doctoral dissertation]. Loughborough University; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/2134/35605.
Council of Science Editors:
Al-Saedi HM. Mathematical modelling of solid tumour growth : a Dynamical Density Functional Theory-based model. [Doctoral Dissertation]. Loughborough University; 2018. Available from: http://hdl.handle.net/2134/35605
University of Illinois – Urbana-Champaign
17.
Corbin, Elise.
Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.
Degree: PhD, 0133, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/46832
► Cancer is an intricate disease that stems from a number of different mutations in a cell. These mutations often control the cellular growth and proliferation,…
(more)
▼ Cancer is an intricate disease that stems from a number of different mutations in a
cell. These mutations often control the cellular
growth and proliferation, a hallmark of cancer, and give rise to many altered biophysical properties. There exists a complex relationship between the behavior of a
cell, its physical properties, and its surrounding environment. Knowledge gleaned from cellular biomechanics can lead to an improved understanding of disease progression and provide methods to target it. There are many studies that look at biophysical changes on a large population level, though there is much information that is lost by treating populations as homogeneous in properties and
cell cycle phase. Biophysical studies on individual cells can link mechanics with function through coordination with the
cell cycle, which is a fundamental physiological process that is crucial for understanding cellular physiology and metabolism. Development of more precise, reliable, and versatile measurement techniques will provide a greater understanding the physical properties of a
cell and how they affect its behavior. Microelectromechanical systems (MEMS) technology can provide tools for manipulating, processing, and analyzing single cells, thus enabling detailed analyses of their biophysical properties.
Growth is a vital element of the
cell cycle, and
cell mass homeostasis ensures that the
cell mass and
cell cycle transitions are coordinately linked. An accurate measurement of
growth throughout the
cell cycle is fundamental to understanding mechanisms of cellular proliferation in cancer.
Growth can be identified through many ways; however,
cell mass has been unexplored until the recent development of cantilever-type MEMS devices for mass sensing through resonant frequency shift. Measuring the dependency of
growth rate on cellular mass may help explain the coordination and regulation of the
cell cycle. However, MEMS mass sensing devices still require further development and characterization in order to reliably investigate long-term
cell growth over the duration of the
cell cycle.
This dissertation focuses on the use of MEMS resonant pedestal sensors for measuring the mass and
growth rate of single cancer cells. This work included characterization and improvement of the sensors to address current challenges in the measurement of long-term
growth rate. The MEMS resonant pedestal sensors were first used to measure physical properties of biomaterials, including the micromechanical properties of hydrogels through verification of stiffness effect on mass measurements. Before studying live cells, modifications to the fabrication process were introduced to improve
cell capture and retention. These include integration of an on-chip microfluidic system for delivery of fluids during mass measurements and the micro-patterning of sensor surfaces for select functionalization and passivation. These modifications enable long-term measurement of the changes in mass of normal and cancerous cells over time. This is the first investigation of the…
Advisors/Committee Members: Bashir, Rashid (advisor), King, William P. (Committee Chair), Bashir, Rashid (committee member), Wagoner Johnson, Amy J. (committee member), Prasanth, Supriya G. (committee member), Kong, Hyun Joon (committee member).
Subjects/Keywords: Micromechanical Sensors; Breast Cancer; Cell Mass; Cell Growth Rate; Cell Stiffness; Micro-Patterning; Long-Term Growth
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APA (6th Edition):
Corbin, E. (2014). Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/46832
Chicago Manual of Style (16th Edition):
Corbin, Elise. “Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.” 2014. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed April 11, 2021.
http://hdl.handle.net/2142/46832.
MLA Handbook (7th Edition):
Corbin, Elise. “Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors.” 2014. Web. 11 Apr 2021.
Vancouver:
Corbin E. Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/2142/46832.
Council of Science Editors:
Corbin E. Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/46832
University of Pretoria
18.
[No author].
Effect of cytokinin and gibberellin on potato tuber
dormancy
.
Degree: 2008, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-07302008-164519/
► The effect of cytokinin and gibberellin, and in particular a combination of the two, on termination of dormant potato tubers was investigated. The objective was…
(more)
▼ The effect of cytokinin and gibberellin, and in
particular a combination of the two, on termination of dormant
potato tubers was investigated. The objective was to effectively
terminate dormancy through the external application of a
combination of cytokinin and gibberellin. Freshly harvested tubers
were treated and either cut at the stolon end with the apical
portions placed on moist cotton wool, or left intact and dry. Tuber
segments treated with a high concentration of cytokinin (0.1g.Lˉ¹)
or a combination of cytokinin and gibberellin sprouted within 5
days, whereas high gibberellin concentrations (0.1g.Lˉ¹) stimulated
sprouting within 9 days. Untreated tuber segments supplied only
with moisture terminated dormancy later than hormonal treated
tubers, but much earlier than segments that were kept dry. Tuber
segments treated with a combination of cytokinin and gibberellin,
or a high concentration of gibberellin (0.1g.Lˉ¹), produced more
and longer sprouts than tubers treated with only cytokinin
(0.1g.Lˉ¹) or a low concentration of gibberellin (0.005g.Lˉ¹).
Sprouts on tuber segments treated with a combination of cytokinin
and gibberellin attained maximum sprout
growth rate nine days after
treatment, but thereafter the
growth rate decreased. This decrease
may be a consequence of closed plasmodesmata although membrane
permeability and its affect on assimilate availability may play a
role. This phenomenon deserves further research attention. Removal
of wound periderm did not reactivate sprout
growth. The wounding of
tubers by removing a portion at the stolon end and supplying
moisture greatly enhanced the termination of dormancy and
subsequent sprout
growth, indicating that the availability of water
may be a factor in initiation of sprouts. The results are
compatible with the hypothesis that cells in dormant buds are
arrested in the G1 phase of the
cell cycle. Cytokinin is needed to
initiate
cell cycling, but gibberellin is also needed to initiate
and maintain
cell growth. These two
growth regulators are also
involved in the opening of the plasmodesmata as well as the
creation of new plasmodesmata witch would establish communication
between the apical meristem and the rest of the
tuber.
Advisors/Committee Members: Prof P S Hammes (advisor).
Subjects/Keywords: Growth rate;
Plasmodesmata;
Sprout growth;
Cell cycle;
Gibberellin;
Cytokinin;
Solanum tuberosum;
UCTD
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MLA ·
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CSE |
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APA (6th Edition):
author], [. (2008). Effect of cytokinin and gibberellin on potato tuber
dormancy
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-07302008-164519/
Chicago Manual of Style (16th Edition):
author], [No. “Effect of cytokinin and gibberellin on potato tuber
dormancy
.” 2008. Masters Thesis, University of Pretoria. Accessed April 11, 2021.
http://upetd.up.ac.za/thesis/available/etd-07302008-164519/.
MLA Handbook (7th Edition):
author], [No. “Effect of cytokinin and gibberellin on potato tuber
dormancy
.” 2008. Web. 11 Apr 2021.
Vancouver:
author] [. Effect of cytokinin and gibberellin on potato tuber
dormancy
. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Apr 11].
Available from: http://upetd.up.ac.za/thesis/available/etd-07302008-164519/.
Council of Science Editors:
author] [. Effect of cytokinin and gibberellin on potato tuber
dormancy
. [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-07302008-164519/
University of Alberta
19.
Lin, Wan-Ying.
Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina.
Degree: MS, Department of Physiology, 2011, University of Alberta
URL: https://era.library.ualberta.ca/files/7h149r27f
► The developing chick retina is an extrapituitary site of growth hormone (GH) synthesis and action. GH, GH receptor (GHR) and their mRNAs are present in…
(more)
▼ The developing chick retina is an extrapituitary site
of growth hormone (GH) synthesis and action. GH, GH receptor (GHR)
and their mRNAs are present in the neural retina when the neural
cells are undergoing proliferation and differentiation during early
embryogenesis. It is thus likely that GH acts as an autocrine or
paracrine in this location. The present study shows that
intra-vitreal injection of a chick GH (cGH) small interfering RNA
(siRNA) into the eyes of early embryos [embryonic day (ED) 4]
suppresses GH expression in the neural retina and increases the
incidence of spontaneous retinal cell death. Our current work also
demonstrates a reduction of local IGF-1 expression after retinal GH
gene knockdown, suggesting that GH action in retinal cells is
regulated through IGF-1 signalling. These results demonstrate that
retinal GH is an autocrine/paracrine hormone that acts as a
neuroprotective factor in the retina of chick
embryos.
Subjects/Keywords: growth hormone (GH); autocrine; insulin-like growth factor-1 (IGF-1); cell apoptosis; paracrine
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lin, W. (2011). Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/7h149r27f
Chicago Manual of Style (16th Edition):
Lin, Wan-Ying. “Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina.” 2011. Masters Thesis, University of Alberta. Accessed April 11, 2021.
https://era.library.ualberta.ca/files/7h149r27f.
MLA Handbook (7th Edition):
Lin, Wan-Ying. “Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina.” 2011. Web. 11 Apr 2021.
Vancouver:
Lin W. Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina. [Internet] [Masters thesis]. University of Alberta; 2011. [cited 2021 Apr 11].
Available from: https://era.library.ualberta.ca/files/7h149r27f.
Council of Science Editors:
Lin W. Retinal Growth Hormone: An Autocrine/paracrine in the
Developing Chick Retina. [Masters Thesis]. University of Alberta; 2011. Available from: https://era.library.ualberta.ca/files/7h149r27f
University of Pretoria
20.
Rossouw, Jan Adriaan.
Effect of
cytokinin and gibberellin on potato tuber dormancy.
Degree: Plant Production and Soil
Science, 2008, University of Pretoria
URL: http://hdl.handle.net/2263/26858
► The effect of cytokinin and gibberellin, and in particular a combination of the two, on termination of dormant potato tubers was investigated. The objective was…
(more)
▼ The effect of cytokinin and gibberellin, and in
particular a combination of the two, on termination of dormant
potato tubers was investigated. The objective was to effectively
terminate dormancy through the external application of a
combination of cytokinin and gibberellin. Freshly harvested tubers
were treated and either cut at the stolon end with the apical
portions placed on moist cotton wool, or left intact and dry. Tuber
segments treated with a high concentration of cytokinin (0.1g.Lˉ¹)
or a combination of cytokinin and gibberellin sprouted within 5
days, whereas high gibberellin concentrations (0.1g.Lˉ¹) stimulated
sprouting within 9 days. Untreated tuber segments supplied only
with moisture terminated dormancy later than hormonal treated
tubers, but much earlier than segments that were kept dry. Tuber
segments treated with a combination of cytokinin and gibberellin,
or a high concentration of gibberellin (0.1g.Lˉ¹), produced more
and longer sprouts than tubers treated with only cytokinin
(0.1g.Lˉ¹) or a low concentration of gibberellin (0.005g.Lˉ¹).
Sprouts on tuber segments treated with a combination of cytokinin
and gibberellin attained maximum sprout
growth rate nine days after
treatment, but thereafter the
growth rate decreased. This decrease
may be a consequence of closed plasmodesmata although membrane
permeability and its affect on assimilate availability may play a
role. This phenomenon deserves further research attention. Removal
of wound periderm did not reactivate sprout
growth. The wounding of
tubers by removing a portion at the stolon end and supplying
moisture greatly enhanced the termination of dormancy and
subsequent sprout
growth, indicating that the availability of water
may be a factor in initiation of sprouts. The results are
compatible with the hypothesis that cells in dormant buds are
arrested in the G1 phase of the
cell cycle. Cytokinin is needed to
initiate
cell cycling, but gibberellin is also needed to initiate
and maintain
cell growth. These two
growth regulators are also
involved in the opening of the plasmodesmata as well as the
creation of new plasmodesmata witch would establish communication
between the apical meristem and the rest of the tuber.
Advisors/Committee Members: Prof P S Hammes (advisor).
Subjects/Keywords: Growth
rate;
Plasmodesmata; Sprout
growth; Cell
cycle;
Gibberellin;
Cytokinin; Solanum
tuberosum;
UCTD
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rossouw, J. A. (2008). Effect of
cytokinin and gibberellin on potato tuber dormancy. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/26858
Chicago Manual of Style (16th Edition):
Rossouw, Jan Adriaan. “Effect of
cytokinin and gibberellin on potato tuber dormancy.” 2008. Masters Thesis, University of Pretoria. Accessed April 11, 2021.
http://hdl.handle.net/2263/26858.
MLA Handbook (7th Edition):
Rossouw, Jan Adriaan. “Effect of
cytokinin and gibberellin on potato tuber dormancy.” 2008. Web. 11 Apr 2021.
Vancouver:
Rossouw JA. Effect of
cytokinin and gibberellin on potato tuber dormancy. [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/2263/26858.
Council of Science Editors:
Rossouw JA. Effect of
cytokinin and gibberellin on potato tuber dormancy. [Masters Thesis]. University of Pretoria; 2008. Available from: http://hdl.handle.net/2263/26858
University of Guelph
21.
Irwin, David.
THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio).
Degree: MS, Department of Integrative Biology, 2011, University of Guelph
URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3185
► Insulin-like growth factors (IGF) are known paracrine/autocrine regulators of ovarian development in teleosts. Initial studies investigated the hormonal and intracellular signal cascades involved in regulating…
(more)
▼ Insulin-like
growth factors (IGF) are known paracrine/autocrine regulators of ovarian development in teleosts. Initial studies investigated the hormonal and intracellular signal cascades involved in regulating the expression of ovarian-derived IGFs in zebrafish (Danio rerio). Quantitative real-time PCR was used to quantify the expression of igf3, igf2a, and igf2b in full grown immature (FG; 0.57-0.65 mm) and mid-vitellogenic (MV; 0.45-0.56 mm) follicles. Addition of the gonadotropin analogue human chorionic gonadotropin (hCG) and the adenylate cyclase activator forskolin increased igf3 expression in FG and MV follicles, but had no effect on igf2a or igf2b expression. The effects of hCG were blocked by the addition of the protein kinase A inhibitor H-89. Pituitary adenylate cyclase activating peptide stimulated a small increase in igf3 expression in FG follicles, while
growth hormone and salmon gonadotropin releasing hormone had no effect on igf3, igf2a, or igf2b expression. Treatment with melittin, prostaglandin F2α, and prostaglandin E2 inhibited igf3 and igf2b expression in FG follicles whereas the protein kinase C activators, PMA and A23187, significantly inhibited igf3, igf2a, igf2b expression in FG and MV follicles. Secondary studies investigated the involvement of ovarian-derived IGFs in mediating the ovarian actions of gonadotropins on
cell survival and steroidogenesis. Treatment of FG follicles with recombinant human IGF-I, hCG, or forskolin inhibited the induction of caspase-3/7 activity, which was used as a measure of apoptosis. The effects of hCG and forskolin on caspase-3/7 were attenuated by co-treatment with NVP-AEW54, an IGF-I receptor antagonist. hCG increased production of the maturation-inducing steroid 17α, 20β-dihydroxy-4-pregnen-3-one and co-treatment with NVP-AEW541 had no effect. These results suggest there is a high degree of hormonal specificity in regulating IGFs in the zebrafish ovary and the ovarian-derived IGFs, presumably IGF-III, are downstream mediators of gonadotropin-dependent
cell survival, but are not involved in gonadotropin-induced steroidogenesis.
Advisors/Committee Members: Van Der Kraak, Glen (advisor).
Subjects/Keywords: Insulin-like growth factors; IGF-III; Ovarian development; Gonadotropins; Prostaglandins; Growth hormone; Steroidogenesis; Cell survival
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Irwin, D. (2011). THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio). (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3185
Chicago Manual of Style (16th Edition):
Irwin, David. “THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio).” 2011. Masters Thesis, University of Guelph. Accessed April 11, 2021.
https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3185.
MLA Handbook (7th Edition):
Irwin, David. “THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio).” 2011. Web. 11 Apr 2021.
Vancouver:
Irwin D. THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio). [Internet] [Masters thesis]. University of Guelph; 2011. [cited 2021 Apr 11].
Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3185.
Council of Science Editors:
Irwin D. THE REGULATION AND FUNCTION OF THE OVARIAN-DERIVED INSULIN-LIKE GROWTH FACTOR SYSTEM IN ZEBRAFISH (Danio rerio). [Masters Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3185
University of Toronto
22.
Wang, Chongda.
Simulation of Cell Growth in High-pressure Foam Injection Molding.
Degree: 2017, University of Toronto
URL: http://hdl.handle.net/1807/79464
► In this work, visualized experiments were conducted to reveal the fundamental mechanisms of nucleation and growth using high-pressure foam injection molding (HP-FIM) with and without…
(more)
▼ In this work, visualized experiments were conducted to reveal the fundamental mechanisms of nucleation and growth using high-pressure foam injection molding (HP-FIM) with and without mold opening (MO) using a real-time in-situ visualization system. Results from both molding methods showed significant differences in nucleation and growth behavior and final foam morphology when different packing pressures were applied. A simulation strategy is proposed to predict the bubble growth profile in both HP-FIM and HP-FIM + MO. The simulation is composed of three main components 1. The Cell model, 2. Material and transport properties which are functions of temperature, pressure, and blowing agent concentration, 3. The Simha-Somcynsky equation of state. Simulation was conducted on both molding methods. The predicted growth profiles for both trials were in quantitative agreement with experimental findings. Sensitivity analysis was also conducted, the resulted growth profiles under different conditions were in qualitative agreement with the literature.
M.A.S.
Advisors/Committee Members: Park, Chul B., Mechanical and Industrial Engineering.
Subjects/Keywords: Foam injection molding; Simulation of bubble growth; Simulation of cell growth; 0548
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, C. (2017). Simulation of Cell Growth in High-pressure Foam Injection Molding. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/79464
Chicago Manual of Style (16th Edition):
Wang, Chongda. “Simulation of Cell Growth in High-pressure Foam Injection Molding.” 2017. Masters Thesis, University of Toronto. Accessed April 11, 2021.
http://hdl.handle.net/1807/79464.
MLA Handbook (7th Edition):
Wang, Chongda. “Simulation of Cell Growth in High-pressure Foam Injection Molding.” 2017. Web. 11 Apr 2021.
Vancouver:
Wang C. Simulation of Cell Growth in High-pressure Foam Injection Molding. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/1807/79464.
Council of Science Editors:
Wang C. Simulation of Cell Growth in High-pressure Foam Injection Molding. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/79464
Columbia University
23.
Hayano, Miki.
Probing cell death mechanisms with chemical and genetic tools.
Degree: 2015, Columbia University
URL: https://doi.org/10.7916/D8610Z52
► Understanding of cell death mechanisms is important to identifying therapeutic approaches to treat excess cell growth, as seen in tumors, or to inhibit excess cell…
(more)
▼ Understanding of cell death mechanisms is important to identifying therapeutic approaches to treat excess cell growth, as seen in tumors, or to inhibit excess cell death, as seen in neurodegenerative disease and ischemia. In the first part of this work, we aim to extend the understanding of a non-apoptotic cell death phenotype, ferroptosis, through use of a genome-wide siRNA screen. We identified knockdown of CARS, or cysteinyl-tRNA synthetase, as an inhibitor of erastin-induced ferroptosis. Loss of CARS led to upregulation of the transsulfuration pathway, where methionine is used as the source of sulfur for cysteine synthesis, as a suppressive mechanism. Upregulation of the transsulfuration pathway may serve as a biomarker to identify tumor types that may be insensitive to ferroptosis-inducing therapeutics. On the other hand, induction of the transsulfuration pathway may be beneficial in disease contexts that involve excess cell death. In the second part of this work, we elucidate the mechanism of action of a small molecule Mdm2 inhibitor, or MEL. Mdm2 is a negative inhibitor of p53; therefor, an inhibitor of Mdm2 may be useful in treating tumors driven by Mdm2 overexpression. We found MEL to inhibit the E3-ligase activity of Mdm2/MdmX heterocomplex, proving to be a useful tool to probe the importance of the heterocomplex in normal physiology and disease development. We also explored the structural scaffold of MEL compounds, an indole, and identified a novel ferroptosis inducer, increasing the chemical toolbox available to study ferroptosis.
Subjects/Keywords: Cells; Cytology; Death (Biology); Cells – Growth; Cells – Growth – Regulation; Cell death; Biology
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hayano, M. (2015). Probing cell death mechanisms with chemical and genetic tools. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8610Z52
Chicago Manual of Style (16th Edition):
Hayano, Miki. “Probing cell death mechanisms with chemical and genetic tools.” 2015. Doctoral Dissertation, Columbia University. Accessed April 11, 2021.
https://doi.org/10.7916/D8610Z52.
MLA Handbook (7th Edition):
Hayano, Miki. “Probing cell death mechanisms with chemical and genetic tools.” 2015. Web. 11 Apr 2021.
Vancouver:
Hayano M. Probing cell death mechanisms with chemical and genetic tools. [Internet] [Doctoral dissertation]. Columbia University; 2015. [cited 2021 Apr 11].
Available from: https://doi.org/10.7916/D8610Z52.
Council of Science Editors:
Hayano M. Probing cell death mechanisms with chemical and genetic tools. [Doctoral Dissertation]. Columbia University; 2015. Available from: https://doi.org/10.7916/D8610Z52
24.
Poucet, Théo.
The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale.
Degree: Docteur es, Biologie Végétale, 2020, Bordeaux; Universidad del País Vasco. Facultad de ciencias
URL: http://www.theses.fr/2020BORD0079
► L'ammonium (NH4+) est une source d'azote d'un grand intérêt dans le cadre d'une agriculture durable. Son application en champs avec des inhibiteurs de nitrification s’est…
(more)
▼ L'ammonium (NH4+) est une source d'azote d'un grand intérêt dans le cadre d'une agriculture durable. Son application en champs avec des inhibiteurs de nitrification s’est montré efficace pour limiter les pertes de N par rapport à l'utilisation de nitrate (NO3-). NH4+ est un intermédiaire commun impliqué dans de nombreuses voies métaboliques. Cependant, des concentrations élevées peuvent conduire à une situation de stress chez la plante provoquant un « syndrome ammoniacal », caractérisé par une croissance réduite. Ces symptômes sont causés par la combinaison, entre autres, d'une reprogrammation métabolique, d'une perturbation de la photosynthèse, d'une dérégulation du pH et d'un déséquilibre ionique. De nombreuses études ont décrit la façon dont la plante s’adapte à la nutrition ammoniacale. Cependant, le stade de développement des organes a été souvent négligé.Pour combler cette lacune, dans le premier chapitre nous étudions comment le métabolisme s’adapte en fonction de la position des feuilles sur l'axe vertical de plants de tomates (Solanum lycopersicum) cultivées en présence de NH4+, NO3- ou NO3NH4. Nous avons disséqué la composition de la biomasse foliaire et le métabolisme grâce à une analyse complète des métabolites, ions et activités enzymatiques. Nos résultats montrent que l'ajustement métabolique du C et du N en fonction de la source d'azote était plus intense chez les feuilles âgées par rapport au plus jeunes. Surtout, nous révélons un compromis entre l'accumulation de NH4+ et l'assimilation afin de préserver les jeunes feuilles du stress ammoniacal. Par ailleurs, les plantes alimentées en NH4+ présentaient un réarrangement des squelettes carbonés impliquant un coût énergétique élevé. Nous expliquons une telle réallocation par l'action du pH-stat biochimique, pour compenser la production différentielle de protons dépendante de la forme azotée fournie.La nutrition ammoniacale peut limiter l'expansion cellulaire. Entre autres, la croissance cellulaire dépend largement de la pression interne exercée par la vacuole sur la paroi cellulaire. Cependant, l’impact du stress ammoniacal sur la vacuole a été rarement abordé. Dans le second chapitre, nous évaluons l'effet de la nutrition ammoniacale sur le développement des feuilles en se focalisant sur l'expansion et le métabolisme vacuolaire. Pour cela, nous avons suivi le développement d’une feuille depuis son apparition jusqu'à son expansion complète avec du NH4+ ou NO3- comme seule source d'azote. Nous avons d’abord mis en évidence que la réduction de l’expansion cellulaire en nutrition ammoniacal était associée à des vacuole plus petite et aussi plus acide que celles recevant du NO3-. De plus, un modèle a été construit pour prédire l'équilibre thermodynamique de différentes espèces solubles de part et d’autre du tonoplaste. Le modèle intègre les volumes subcellulaires, les gradients électrochimiques et la formation de complexe ionique dans la vacuole afin de prédire les concentrations subcellulaires des ions, acides organiques et sucres mesurée dans la feuille.…
Advisors/Committee Members: Dieuaide-Noubhani, Martine (thesis director), Marino Bilbao, Daniel (thesis director).
Subjects/Keywords: Vacuole; Metabolisme; Growth; Ammonium; Nitrate; Modelisation; Vacuole; Metabolism; Cell growth; Ammonium; Nitrate; Modelling
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Manager
APA (6th Edition):
Poucet, T. (2020). The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale. (Doctoral Dissertation). Bordeaux; Universidad del País Vasco. Facultad de ciencias. Retrieved from http://www.theses.fr/2020BORD0079
Chicago Manual of Style (16th Edition):
Poucet, Théo. “The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale.” 2020. Doctoral Dissertation, Bordeaux; Universidad del País Vasco. Facultad de ciencias. Accessed April 11, 2021.
http://www.theses.fr/2020BORD0079.
MLA Handbook (7th Edition):
Poucet, Théo. “The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale.” 2020. Web. 11 Apr 2021.
Vancouver:
Poucet T. The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale. [Internet] [Doctoral dissertation]. Bordeaux; Universidad del País Vasco. Facultad de ciencias; 2020. [cited 2021 Apr 11].
Available from: http://www.theses.fr/2020BORD0079.
Council of Science Editors:
Poucet T. The energy cost of primary metabolism and vacuole expansion : Central to shape tomato leaf development under ammonium nutrition : Le coût énergétique du métabolisme primaire et de l'expansion vacuolaire : Des acteurs centraux pour le développement des feuilles de tomates en nutrition ammoniacale. [Doctoral Dissertation]. Bordeaux; Universidad del País Vasco. Facultad de ciencias; 2020. Available from: http://www.theses.fr/2020BORD0079
Massey University
25.
Zaidi, Ali Ashher.
Mathematics of cell growth.
Degree: PhD, Mathematics, 2014, Massey University
URL: http://hdl.handle.net/10179/6942
► We present a model that describes growth, division and death of cells structured by size. Here, size can be interpreted as DNA content or physical…
(more)
▼ We present a model that describes growth, division and death of cells structured
by size. Here, size can be interpreted as DNA content or physical
size. The model is an extension of that studied by Hall and Wake [24] and
incorporates the symmetric as well as the asymmetric division of cells.
We first consider the case of symmetric cell division. This leads to an
initial boundary value problem that involves a first-order linear PDE with a
functional term. We study the separable solution to this problem which plays
an important role in the long term behaviour of solutions. We also derive a
solution to the problem for arbitrary initial cell distributions. The method
employed exploits the hyperbolic character of the underlying differential operator,
and the advanced nature of the functional argument to reduce the
problem to a sequence of simple Cauchy problems. The existence of solutions
for arbitrary initial distributions is established along with uniqueness.
The asymptotic relationship with the separable solution is established, and
because the solution is known explicitly, higher order terms in the asymptotics
can be obtained. Adding variability to the growth rate of cells leads
to a modified Fokker-Planck equation with a functional term. We find the
steady size distribution solution to this equation. We also obtain a constructive
existence and uniqueness theorem for this equation with an arbitrary
initial size-distribution and with a no-flux condition.
We then proceed to study the binary asymmetric division of cells. This
leads to an initial boundary value problem that involves a first-order linear
PDE with two functional terms. We find and prove the unimodality of the
steady size distribution solution to this equation. The existence of higher
eigenfunctions is also discussed. Adding stochasticity to the growth rate of
cells yields a second-order functional differential equation with two non-local
terms.
These problems, being a particular kind of functional differential equations
exhibit unusual characteristics. Although the associated boundary
value problems are well-posed, the spectral problems that arise by separating
the variables, cannot be easily shown to have a complete set of eigenfunctions
or the usual orthogonality properties.
Subjects/Keywords: Cells;
Growth;
Cell growth;
Mathematical models;
Research Subject Categories::MATHEMATICS::Applied mathematics
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APA (6th Edition):
Zaidi, A. A. (2014). Mathematics of cell growth. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/6942
Chicago Manual of Style (16th Edition):
Zaidi, Ali Ashher. “Mathematics of cell growth.” 2014. Doctoral Dissertation, Massey University. Accessed April 11, 2021.
http://hdl.handle.net/10179/6942.
MLA Handbook (7th Edition):
Zaidi, Ali Ashher. “Mathematics of cell growth.” 2014. Web. 11 Apr 2021.
Vancouver:
Zaidi AA. Mathematics of cell growth. [Internet] [Doctoral dissertation]. Massey University; 2014. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10179/6942.
Council of Science Editors:
Zaidi AA. Mathematics of cell growth. [Doctoral Dissertation]. Massey University; 2014. Available from: http://hdl.handle.net/10179/6942
Virginia Tech
26.
Gudenschwager Basso, Erwin Kristobal Felipe.
Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma.
Degree: PhD, Biomedical and Veterinary Sciences, 2018, Virginia Tech
URL: http://hdl.handle.net/10919/97990
► Throughout gestation, the blood vessel network of the placenta is formed sequentially by processes known as vasculogenesis and angiogenesis, which together meet the needs of…
(more)
▼ Throughout gestation, the blood vessel network of the placenta is formed sequentially by processes known as vasculogenesis and angiogenesis, which together meet the needs of the growing fetus. Normal placental angiogenesis is critical to support adequate fetal
growth and assure the health of the offspring. Proper angiogenesis requires precise regulation of expression of agents that modulate this process; otherwise, pathologies of pregnancy such as preeclampsia may occur. The placenta is composed of different layers of tissue, including the lamellar (LZ), junctional, and glandular zones, each with a vascular morphology attuned to its function. We hypothesized that higher expression of pro-angiogenic factors is associated with increased morphological metrics in the LZ, the major vascularized zone. Thus, we aimed to characterize the major changes in morphology and vascular development in the placenta throughout pregnancy in cats, alongside a compressive analysis of the expression of major angiogenic factors and their receptors in the placenta, with an emphasis on the identification and interaction of different isoforms of the VEGF family.
Microscopic analysis of tissue specimens from different stages of pregnancy revealed increased thickness of the LZ, especially during early to mid-gestation, at which time the tissue is composed of abundant materno-fetal interdigitations that appears rich in capillaries. VEGF proteins were detected in placental tissue in both fetal and maternal cells of the placenta, suggesting stimulatory interactions between different
cell types to promote
growth and angiogenesis. Gene expression analysis of placenta revealed upregulation of the pro-angiogenic factor VEGF-A in mid-pregnancy, followed by a steady decline toward term, consistent with morphologic changes in the LZ. In contrast, another pro-angiogenic factor, PlGF, showed a marked increase toward term; Flt-1, which acts as a receptor or reservoir for PLGF and VEGF A, was also upregulated at late pregnancy. Increased ratios of PLGF:VEGF-A may contribute to LZ proliferation in the last trimester. These findings are consistent with the creation of a proangiogenic placental state during gestation. Overall, we expect that this research will help elucidate mechanisms of placental vascularization, which can be applied to the design of improved strategies to treat vascular complications of pregnancy.
Lastly, we applied the tools developed for placental studies to investigate pathologic angiogenesis in cutaneous squamous
cell carcinoma (CSCC), a common skin cancer with major economic and medical impacts in humans and veterinary species. The creation of a new blood supply is essential for
growth and metastasis of many tumor types. The goal of this study was to measure expression of variants of proteins that stimulate angiogenesis or transmit an angiogenic stimulus in feline CSCC. The results were mixed, with differences detected in expression of some regulatory agents and, for others, unexpectedly lower expression in CSSC compared to…
Advisors/Committee Members: Huckle, William R. (committeechair), Clark-Deener, Sherrie (committee member), Eyestone, Willard H. (committee member), Gutierrez, Juan Claudio (committee member).
Subjects/Keywords: Vascular endothelial growth factor; Placental Growth factor; Placenta; Domestic Cat; Angiogenesis; Cutaneous Squamous Cell Carcinoma
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APA (6th Edition):
Gudenschwager Basso, E. K. F. (2018). Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/97990
Chicago Manual of Style (16th Edition):
Gudenschwager Basso, Erwin Kristobal Felipe. “Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma.” 2018. Doctoral Dissertation, Virginia Tech. Accessed April 11, 2021.
http://hdl.handle.net/10919/97990.
MLA Handbook (7th Edition):
Gudenschwager Basso, Erwin Kristobal Felipe. “Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma.” 2018. Web. 11 Apr 2021.
Vancouver:
Gudenschwager Basso EKF. Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma. [Internet] [Doctoral dissertation]. Virginia Tech; 2018. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/10919/97990.
Council of Science Editors:
Gudenschwager Basso EKF. Characterization of the expression of angiogenic factors in the feline placenta during development and in feline cutaneous squamous cell carcinoma. [Doctoral Dissertation]. Virginia Tech; 2018. Available from: http://hdl.handle.net/10919/97990
University of California – Berkeley
27.
Anderson-Furgeson, James Christian.
Polar growth and cell division in Agrobacterium tumefaciens.
Degree: Microbiology, 2016, University of California – Berkeley
URL: http://www.escholarship.org/uc/item/47s6n82k
► Agrobacterium tumefaciens is a rod-shaped gram-negative bacterium, which elongates by unipolar addition of new cell envelope material. Approaching cell division, the growth pole transitions to…
(more)
▼ Agrobacterium tumefaciens is a rod-shaped gram-negative bacterium, which elongates by unipolar addition of new cell envelope material. Approaching cell division, the growth pole transitions to a non-growing old pole, and the division site creates new growth poles in sibling cells. This thesis describes experiments concerning cell division and polar growth in A. tumefaciens. The cell division proteins FtsZ-GFP and FtsA-GFP localize to the growth pole in addition to the Z-ring at the mid-cell. The A. tumefaciens homolog of the Caulobacter crescentus polar organizing protein PopZCc (PopZAt) localizes specifically to growth poles. In contrast, the A. tumefaciens homolog of the C. crescentus polar organelle development protein PodJCc (PodJAt) localizes to the old pole early in the cell cycle and accumulates at the growth pole as the cell cycle proceeds. FtsA and FtsZ also localize to the growth pole for most of the cell cycle prior to Z-ring formation. To further characterize the function of polar localizing proteins, I created a deletion of podJAt . ΔpodJAt cells display ectopic growth poles (branching), growth poles that fail to transition to an old pole, and elongated cells that fail to divide. In ΔpodJAt cells, PopZAt -GFP persists at non-transitioning growth poles post division and also localizes to ectopic growth poles, as expected for a growth pole specific factor. Even though GFP-PodJAt does not localize to the midcell in wild type, deletion of podJAt impacts localization, stability, and function of Z-rings as assayed by localization of FtsA-GFP and FtsZ-GFP. Z-ring defects are further evidenced by minicell production. Together these data indicate that PodJAt is a critical factor for polar growth, and ΔpodJAt displays a cell division phenotype. These results reveal intimate connections between polar growth and cell division in a model system displaying a novel form of cell growth.
Subjects/Keywords: Microbiology; Cellular biology; Agrobacterium; cell cycle; cell division; FtsZ; polar growth; Rhizobiales
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APA (6th Edition):
Anderson-Furgeson, J. C. (2016). Polar growth and cell division in Agrobacterium tumefaciens. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/47s6n82k
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Anderson-Furgeson, James Christian. “Polar growth and cell division in Agrobacterium tumefaciens.” 2016. Thesis, University of California – Berkeley. Accessed April 11, 2021.
http://www.escholarship.org/uc/item/47s6n82k.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Anderson-Furgeson, James Christian. “Polar growth and cell division in Agrobacterium tumefaciens.” 2016. Web. 11 Apr 2021.
Vancouver:
Anderson-Furgeson JC. Polar growth and cell division in Agrobacterium tumefaciens. [Internet] [Thesis]. University of California – Berkeley; 2016. [cited 2021 Apr 11].
Available from: http://www.escholarship.org/uc/item/47s6n82k.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Anderson-Furgeson JC. Polar growth and cell division in Agrobacterium tumefaciens. [Thesis]. University of California – Berkeley; 2016. Available from: http://www.escholarship.org/uc/item/47s6n82k
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
28.
Paradis, Francois.
Intra-follicular growth factors and preovulatory follicle
development in the sow.
Degree: PhD, Department of Agricultural, Food, and Nutritional
Science, 2009, University of Alberta
URL: https://era.library.ualberta.ca/files/pr76f4056
► Pig follicle development is a complex but poorly understood process involving both gonadotrophins and local ovarian factors. A series of studies sought to investigate these…
(more)
▼ Pig follicle development is a complex but poorly
understood process involving both gonadotrophins and local ovarian
factors. A series of studies sought to investigate these
intrafollicular cell-to-cell interactions. Microarray analysis
combined with gene ontology revealed that the oocyte, granulosa and
theca cells each expressed more than 650 potential secreted factors
and receptors, including members of the TGF-β, IGF1, EGF and FGF
families. Using a well-defined in vivo experimental paradigm that
generates follicles and oocytes of different quality, the temporal
expression of several growth factors in the oocyte, granulosa and
theca cells collected from sows during the recruitment and
mid-selection phases, as well as the final selection of the
preovulatory follicle population before and after the LH surge was
studied. IGF1 expression patterns indicated its potential for
modulating granulosa and theca cell function during the selection
stages, and an involvement in creating differences in follicular
quality between the first and second preovulatory wave
post-weaning. Transient up-regulation of AREG and EREG mRNA around
the LH surge, suggested their involvement in ovulation. Results of
a second study investigating TGF-β superfamily expression,
suggested a role for GDF9 in follicle selection through the
up-regulation of TGFBR1 expression, while BMP15 could be involved
in ovulation through the up-regulation of BMPR1B. Expression of
angiogenesis-related genes during follicle development was also
investigated. During mid-selection, ANGPT2 may allow VEGFA and
similar factors to stimulate vascular development or destabilize
the vasculature and cause follicular atresia, while ANGPT1 may be
required in the preovulatory follicle population. Associations
among the transcription factor HIF1A, VEGFA and ANGPT1, suggested
interactions between the ligands in regulation of angiogenesis.
Finally, the effects of the pig oocyte on cumulus cell function was
assessed by co-culturing cumulus cell complexes with or without
denuded oocytes isolated from large oestrogenic follicles. Presence
of oocytes decreased FSHR and increased HSD3B expression,
potentially stimulating progesterone while attenuating oestradiol
production. In conclusion, oocytes were shown to control cumulus
cell function in a way that reflects their specific environment and
further evidence was obtained for a complex network of growth
factors and receptors in the follicle and their involvement in
regulation of pig follicle development.
Subjects/Keywords: oocyte; follicle; gene expression; pig; granulosa cell; ovary; theca cell; growth factors
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APA ·
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APA (6th Edition):
Paradis, F. (2009). Intra-follicular growth factors and preovulatory follicle
development in the sow. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/pr76f4056
Chicago Manual of Style (16th Edition):
Paradis, Francois. “Intra-follicular growth factors and preovulatory follicle
development in the sow.” 2009. Doctoral Dissertation, University of Alberta. Accessed April 11, 2021.
https://era.library.ualberta.ca/files/pr76f4056.
MLA Handbook (7th Edition):
Paradis, Francois. “Intra-follicular growth factors and preovulatory follicle
development in the sow.” 2009. Web. 11 Apr 2021.
Vancouver:
Paradis F. Intra-follicular growth factors and preovulatory follicle
development in the sow. [Internet] [Doctoral dissertation]. University of Alberta; 2009. [cited 2021 Apr 11].
Available from: https://era.library.ualberta.ca/files/pr76f4056.
Council of Science Editors:
Paradis F. Intra-follicular growth factors and preovulatory follicle
development in the sow. [Doctoral Dissertation]. University of Alberta; 2009. Available from: https://era.library.ualberta.ca/files/pr76f4056
University of Oulu
29.
Zheng, A. (Aiping).
All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria.
Degree: 2000, University of Oulu
URL: http://urn.fi/urn:isbn:9514256735
► Abstract All-trans retinoic acid (ATRA) is a derivative of vitamin A. It is able to stimulate neutrophilic differentiation of normal progenitors and acute promyelocytic leukemia…
(more)
▼ Abstract
All-trans retinoic acid (ATRA) is a derivative of vitamin A. It is able to stimulate neutrophilic differentiation of normal progenitors and acute promyelocytic leukemia (APL) cells. Although ATRA-induced differentiation is not observed in any other acute myeloblastic leukemia (AML) subtypes, ATRA is known to be able to inhibit AML blast cell proliferation. The present in vitro study using AML cell lines representing subtypes other than APL focuses on the following questions: (1) Is the inhibitory effect of ATRA on AML cell growth related to apoptosis of cells? (2) Are the effects of ATRA dependent on two important regulators of apoptosis, p53 and Bcl-2? (3) Do mitochondria have any role in mediating the effects of ATRA? ATRA-induced apoptosis in AML cells was observed by morphology, DNA fragmentation, phosphatidylserine externalization, and poly(ADPribose)polymerase (PARP) cleavage. It was a slow event, manifested as DNA cleavage after 48 hours exposure and as morphological apoptosis after 72 hours exposure. The AML cells expressed constitutively p53 as determined by immunohistochemistry, Western blotting and flow cytometry. However, no mutation of TP53 was observed in exons 5 to 8 as analysed with a single strand conformation polymorphism technique. As the flow cytometer analysis showed, most of p53 was in a aberrant conformation, which was not changed into a wild type conformation by ATRA. Two of the cell lines were analysed more specifically in relation to Bcl-2 and mitochondral function: ATRA-induced apoptosis of the cell lines was associated with down-regulation of Bcl-2. Western blotting showed ATRA-induced apoptosis also to be related to the release of cytochrome c from mitochondria into cytosol, resulting in the activation of caspase-3, an apoptotic effector, which was manifested as a cleavage of its substrate PARP. The process was also accompanied by disruption of the mitochondrial membrane potential as determined fluoricytometrically. These results show that ATRA is able to induce apoptosis in AML cells other than APL, and ATRA-induced apoptosis in the AML cells studied is related to the down-regulation of Bcl-2 and the disruption of mitochondrial function, but is independent of the p53 pathway.
Subjects/Keywords: cell growth; programmed cell death; tretinoin
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Export
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APA (6th Edition):
Zheng, A. (. (2000). All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9514256735
Chicago Manual of Style (16th Edition):
Zheng, A (Aiping). “All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria.” 2000. Doctoral Dissertation, University of Oulu. Accessed April 11, 2021.
http://urn.fi/urn:isbn:9514256735.
MLA Handbook (7th Edition):
Zheng, A (Aiping). “All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria.” 2000. Web. 11 Apr 2021.
Vancouver:
Zheng A(. All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria. [Internet] [Doctoral dissertation]. University of Oulu; 2000. [cited 2021 Apr 11].
Available from: http://urn.fi/urn:isbn:9514256735.
Council of Science Editors:
Zheng A(. All-trans retinoic acid-induced apoptosis in acute myeloblastic leukemia cells:with a special emphasis on p53, Bcl-2, and mitochondria. [Doctoral Dissertation]. University of Oulu; 2000. Available from: http://urn.fi/urn:isbn:9514256735
McMaster University
30.
Athar, Mohammad S.
Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes.
Degree: MSc, 2011, McMaster University
URL: http://hdl.handle.net/11375/10969
► The study of growth arrest specific (GAS) genes is critical for our understanding of quiescence cell states. C/EBP-β is a transcriptional activator which is…
(more)
▼ The study of growth arrest specific (GAS) genes is critical for our understanding of quiescence cell states. C/EBP-β is a transcriptional activator which is central to the expression of GAS genes in growth arrested cells. C/EBP-β is involved in the activation of numerous pathways, including mitogenesis, cytokine signaling, stress response, etc. Thus, it requires signaling cues which confer specificity in terms of gene expression. Here we used the p20K gene in chicken embryonic fibroblasts as a model system to study the control mechanisms of GAS genes. p20K is expressed in conditions such as contact inhibition mediated growth arrest and mild hypoxia. Here we explored the control mechanism mediated by ERK2 at the p20K promoter (QRU), as a mode of regulation which confers C/EBP-β binding specificity. In this study we demonstrate that ERK2 is recruited to the QRU in proliferative cells, i.e. where p20K is repressed. Using ChIP analysis we show that ERK2 binds directly to the QRU in proliferative cell states, but not in growth arrested cell conditions. Using a similar approach we demonstrate that ERK2 binding to the QRU is lost in states of hypoxia, where p20K is strongly induced. Furthermore, we show that this interaction is specific to ERK2 and is not observed with the related ERK1 kinase. Lastly, we employed transient expression assays to illustrate that ERK2 acts as a transcriptional repressor of the QRU. Through these experiments we have illustrated that ERK2 mediated transcriptional repression is a novel control mechanism at the QRU which skews C/EBP-β mediated signaling networks in proliferating cells.
Master of Science (MSc)
Advisors/Committee Members: Bedard, Andre, Cameron, Robin, Elliot, Marie, Biology.
Subjects/Keywords: ERK2; p20K; Quiescence; Growth Arrest; C/EBP-B; Transcription; Cell Biology; Cell Biology
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APA (6th Edition):
Athar, M. S. (2011). Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/10969
Chicago Manual of Style (16th Edition):
Athar, Mohammad S. “Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes.” 2011. Masters Thesis, McMaster University. Accessed April 11, 2021.
http://hdl.handle.net/11375/10969.
MLA Handbook (7th Edition):
Athar, Mohammad S. “Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes.” 2011. Web. 11 Apr 2021.
Vancouver:
Athar MS. Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes. [Internet] [Masters thesis]. McMaster University; 2011. [cited 2021 Apr 11].
Available from: http://hdl.handle.net/11375/10969.
Council of Science Editors:
Athar MS. Characterization of ERK2 as a Transcriptional Repressor of Growth Arrest Specific Genes. [Masters Thesis]. McMaster University; 2011. Available from: http://hdl.handle.net/11375/10969
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Open Access Theses and Dissertations
