subject:(cell division)

Humboldt State University

1. Alkhathlan, Manal. The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus.

Degree: MS, Biology, 2015, Humboldt State University

URL: http://hdl.handle.net/10211.3/149801

► The California Blackworm (Lumbriculus variegatus) has great ability to regenerate from small fragments into a new worm depending on two forms of regeneration: epimorphosis and… (more)

▼ The California Blackworm (Lumbriculus variegatus) has great ability to regenerate from small fragments into a new worm depending on two forms of regeneration: epimorphosis and morphallaxis. Both patterns of regeneration work in concert with each other in order to ensure the survival of the organism. Regeneration of the head segments following amputation produces 7-8 new segments; while the number of regenerated tail segments is dependent on the time following amputation. The nature, origin, and position of cells that are involved in formation of new tissues during regeneration in L. variegatus for both head and tail regeneration are not completely known. In this study I???m trying to find the nature, origin, and position of the neoblasts and investigate if the neoblasts that migrate into the cut site are cells derived from differentiated tissues already in the region of the wound by using EdU staining. The results showed that stem cells (neoblasts) are randomly scattered in the body and few in number. Upon injury these cells undergo hyperproliferation and migrate to the wound site for both head and tail regeneration. During head regeneration the results suggested a reprogramming of cells in the cut site upon injury due to the abundance of Na+ in cells in the injury site. Recent studies showed that a change in the cell???s resting potential influences regeneration. In this study, I exposed the worms to the voltage-gated sodium channel blocker tricaine (TMS) and examined somatic regeneration of heads and tails following body transection. Regeneration of worms was examined for 10 days after amputation either in the presence of TMS or in control pond water. Regeneration of both new head and tail body segments was reduced in the presence of TMS, especially in the tail. Head regeneration in the presence of TMS showed defective morphology with no well defined segments. Both effects of sodium transport into the cells in the bud region were examined by CoroNa Green staining, and somatic cell regeneration in the bud by EdU. The number of dividing cells in the bud region for both head and tail TMS-treated worms decreased significantly, although number of dividing cells that were scattered around the remainder of the body didn???t show a significant change compared to control worms. The number of proliferating cells in the bud region showed a strong relationship with the concentration of Na+ in the cells in the bud region. To further investigate the effects of sodium influx on regeneration, monensin, a sodium ionophore, was used in TMS-treated worms. These worms did not show increased regeneration ability compared to TMS-treated worms. In combination, these result suggest that sodium influx via voltage-gated sodium channels has effects on both head and tail regeneration that affects cell migration of dividing cells that are scattered around the body and dividing cells in the bud region. Advisors/Committee Members: O'Gara, Bruce A..

Subjects/Keywords: Cell division

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APA (6^th Edition):

Alkhathlan, M. (2015). The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus. (Masters Thesis). Humboldt State University. Retrieved from http://hdl.handle.net/10211.3/149801

Chicago Manual of Style (16^th Edition):

Alkhathlan, Manal. “The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus.” 2015. Masters Thesis, Humboldt State University. Accessed January 19, 2021. http://hdl.handle.net/10211.3/149801.

MLA Handbook (7^th Edition):

Alkhathlan, Manal. “The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus.” 2015. Web. 19 Jan 2021.

Vancouver:

Alkhathlan M. The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus. [Internet] [Masters thesis]. Humboldt State University; 2015. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10211.3/149801.

Council of Science Editors:

Alkhathlan M. The role of sodium influx via voltage-gated sodium channels in regeneration of Lumbriculus variegatus. [Masters Thesis]. Humboldt State University; 2015. Available from: http://hdl.handle.net/10211.3/149801

University of Guelph

2. Seidel, Laura. Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis.

Degree: MS, Department of Molecular and Cellular Biology, 2020, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21150

► In Escherichia coli the divisome consists of 10 essential proteins, and is responsible for septum formation, constriction of membranes, and synthesis of septal peptidoglycan. The… (more)

Subjects/Keywords: FtsK; Cell Division

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APA (6^th Edition):

Seidel, L. (2020). Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21150

Chicago Manual of Style (16^th Edition):

Seidel, Laura. “Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis.” 2020. Masters Thesis, University of Guelph. Accessed January 19, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21150.

MLA Handbook (7^th Edition):

Seidel, Laura. “Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis.” 2020. Web. 19 Jan 2021.

Vancouver:

Seidel L. Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis. [Internet] [Masters thesis]. University of Guelph; 2020. [cited 2021 Jan 19]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21150.

Council of Science Editors:

Seidel L. Investigating the Structure of the Escherichia coli Divisome Protein FtsK via Covariance Analysis. [Masters Thesis]. University of Guelph; 2020. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/21150

Rutgers University

3. Li, Dongmeng, 1988-. Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development.

Degree: MS, Plant Biology, 2014, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/45329/

► BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE), a novel intrinsic polarity protein, formulates stomatal stem cell polarization and controls asymmetric cell division (ACD) in… (more)

▼ BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE), a novel intrinsic polarity protein, formulates stomatal stem cell polarization and controls asymmetric cell division (ACD) in Arabidopsis stomatal development. Previous analysis on BASL protein subdomains did not fully elucidate its highly dynamic and dually localized pattern (nucleus and cell peripheral polarity) and its biological function in the stomatal ACD cells. From a genome-wide yeast two-hybrid (Y2H) screening, our lab identified two plant-specific families, BIP1 (BASL Interaction Protein Family 1) and BIP2 (BASL Interaction Protein Family 2), both sharing a common plant-specific BID (BASL Interaction Domain) domain. This work focuses on the BIP2 family, particularly BIP2-4, to investigate where this protein is subcellularly localized and whether this gene family plays a role in stomatal ACD and developmental patterning. The physical interaction between BIP2-4 and BASL was further confirmed with pairwise Y2H tests, Bimolecular Fluorescence Complementation (BiFC) and co-expression assays in tobacco leaves. BiFC assays also demonstrated that in addition to interacting with BASL, BIP2-4 also interacts with the MAPKKK YDA, a recently established polarity protein that interacts with BASL. Intriguingly, the interactions between BASL and BIP2-4 and between YDA and BIP2-4 triggered spontaneous protein co-polarization, suggesting a positive role of BIP2-4 in polarity formation. To genetically investigate the biological functions of the BIP2 family, the new genome editing/mutagenesis technology, CRISPR, was tested and successfully established in the Arabidopsis stomatal system. The employment of this technology provided an efficient solution to overcome genetic redundancy within the BIP2 family for further functional analysis. The BIP2 family has a potential link to the Phosphoinositide (PI) signaling pathway, due to the presence of multiple PI-binding signature domains in their sequences. To connect the possible PI signaling to BIP2, the subcellular localization of BIP2-4 subdomains and three previously established PI biosensors that specifically bind to PI3P, PI4P, or PI(4, 5)P2 were examined and compared in the stomata lineage cells. Advisors/Committee Members: Dong, Juan (chair), Maliga, Pal (internal member), Gallavotti, Andrea (internal member).

Subjects/Keywords: Cell division; Arabidopsis

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APA (6^th Edition):

Li, Dongmeng, 1. (2014). Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/45329/

Chicago Manual of Style (16^th Edition):

Li, Dongmeng, 1988-. “Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development.” 2014. Masters Thesis, Rutgers University. Accessed January 19, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/45329/.

MLA Handbook (7^th Edition):

Li, Dongmeng, 1988-. “Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development.” 2014. Web. 19 Jan 2021.

Vancouver:

Li, Dongmeng 1. Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development. [Internet] [Masters thesis]. Rutgers University; 2014. [cited 2021 Jan 19]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45329/.

Council of Science Editors:

Li, Dongmeng 1. Functional characterization of bip2-4, a basl interacting protein, in arabidopsis stomatal development. [Masters Thesis]. Rutgers University; 2014. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/45329/

University of Alberta

4. Ataeian, Maryam. Identification and Characterization of mel-43 in C. elegans.

Degree: MS, Department of Biological Sciences, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/cv43nx322

► In the newly fertilized embryo, meiotic and mitotic spindles form consecutively within the same egg cytoplasm. The order of events during this transition is controlled,… (more)

Subjects/Keywords: meiosis; C. elegans; cell division

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APA (6^th Edition):

Ataeian, M. (2013). Identification and Characterization of mel-43 in C. elegans. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cv43nx322

Chicago Manual of Style (16^th Edition):

Ataeian, Maryam. “Identification and Characterization of mel-43 in C. elegans.” 2013. Masters Thesis, University of Alberta. Accessed January 19, 2021. https://era.library.ualberta.ca/files/cv43nx322.

MLA Handbook (7^th Edition):

Ataeian, Maryam. “Identification and Characterization of mel-43 in C. elegans.” 2013. Web. 19 Jan 2021.

Vancouver:

Ataeian M. Identification and Characterization of mel-43 in C. elegans. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Jan 19]. Available from: https://era.library.ualberta.ca/files/cv43nx322.

Council of Science Editors:

Ataeian M. Identification and Characterization of mel-43 in C. elegans. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/cv43nx322

Oregon State University

5. Clothier, Galen Edward. Cyclic uptake of calcium-45 by dividing sea urchin eggs.

Degree: PhD, Zoology, 1960, Oregon State University

URL: http://hdl.handle.net/1957/49245

Subjects/Keywords: Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clothier, G. E. (1960). Cyclic uptake of calcium-45 by dividing sea urchin eggs. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/49245

Chicago Manual of Style (16^th Edition):

Clothier, Galen Edward. “Cyclic uptake of calcium-45 by dividing sea urchin eggs.” 1960. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/49245.

MLA Handbook (7^th Edition):

Clothier, Galen Edward. “Cyclic uptake of calcium-45 by dividing sea urchin eggs.” 1960. Web. 19 Jan 2021.

Vancouver:

Clothier GE. Cyclic uptake of calcium-45 by dividing sea urchin eggs. [Internet] [Doctoral dissertation]. Oregon State University; 1960. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/49245.

Council of Science Editors:

Clothier GE. Cyclic uptake of calcium-45 by dividing sea urchin eggs. [Doctoral Dissertation]. Oregon State University; 1960. Available from: http://hdl.handle.net/1957/49245

University of Saskatchewan

6. Sharma, Kusum. Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape.

Degree: 2020, University of Saskatchewan

URL: http://hdl.handle.net/10388/12702

► DivIVA plays multifaceted roles in Gram-positive organisms by associating with various cell division and non-cell division proteins. While the interaction of DivIVA with other proteins… (more)

▼ DivIVA plays multifaceted roles in Gram-positive organisms by associating with various cell division and non-cell division proteins. While the interaction of DivIVA with other proteins has been studied in many Gram-positive bacteria, no information is available about DivIVA- associating proteins in E. faecalis. This research reports a novel DivIVAEf interacting protein named EF1025 (encoded by EF1025) (confirmed using Bacterial Two-Hybrid, Glutathione S-Transferase pull-down, and co-immunoprecipitation assays) that affects cell length and morphology in E. faecalis. EF1025 is predominantly conserved in Gram-positive bacteria and contains a conserved N-terminal DNA binding Helix-turn-Helix (HTH) domain and two Cystathionine β-Synthase (CBS) domains located centrally and at the C-terminus. The protein, EF1025, oligomerizes to form a higher-order oligomer and the two CBS domains are responsible for its self-interaction. Viable cells were recovered after insertional inactivation or deletion of EF1025 only through complementation of EF1025 in trans. These cells were longer than the average length of E. faecalis cells and had distorted shapes. Overexpression of EF1025 also resulted in cell elongation but had no effect on cell shape. Immuno-staining revealed comparable localization patterns of EF1025 and DivIVAEf in the later stages of division in E. faecalis cells. The EF1025 homologue in Bacillus subtilis, CcpN, is a transcriptional repressor in Bacillus subtilis. In the presence of glucose, CcpN binds to the promoter region of gapB and pckA and downregulates their expression. CcpN interacted with DivIVA of B. subtilis in B2H and GST-pull down assays. A heterologous interaction between EF1025 and DivIVABs was also identified in a GST-pull down assay. Insertional inactivation of ccpN leads to cell elongation and growth of cells in straight chains. These findings suggest an additional function of CcpN in B. subtilis, therefore, CcpN is a dual function performing protein involved in both gluconeogenesis and cell elongation. E. faecalis contains homologues of divisome proteins FtsZ, FtsA, FtsK, FtsQ, FtsL, FtsI and FtsB, however, the cell division interactome of E. faecalis, by contrast, is not presently known. This thesis also presents the unique interactome of E. faecalis divisome proteins (i.e. FtsZEf, FtsAEf, FtsQEf, FtsLEf, FtsIEf, FtsWEf, DivIVAEf, and FtsBEf), established using Bacterial-two hybrid system. The interaction of FtsA with FtsI, FtsL, and FtsZ, is common among E. faecalis, S. pneumoniae and S. aureus cell division interactomes. One unique interaction i.e. FtsZEf-FtsIEf was identified in E. faecalis cell division interactome. While studying the divisome interactome of E. faecalis, it was observed that EF1025 is not a part of the divisome machinery in E. faecalis as it did not interact with any divisome protein except DivIVAEf. Advisors/Committee Members: Xiao, Wei, White, Aaron, Napper, Scott, Howard, Peter.

Subjects/Keywords: Cell division; Enterococcus faecalis

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APA (6^th Edition):

Sharma, K. (2020). Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/12702

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sharma, Kusum. “Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape.” 2020. Thesis, University of Saskatchewan. Accessed January 19, 2021. http://hdl.handle.net/10388/12702.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sharma, Kusum. “Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape.” 2020. Web. 19 Jan 2021.

Vancouver:

Sharma K. Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape. [Internet] [Thesis]. University of Saskatchewan; 2020. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10388/12702.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sharma K. Characterization of a “hypothetical protein”, EF1025, from Enterococcus faecalis: role in cell length and shape. [Thesis]. University of Saskatchewan; 2020. Available from: http://hdl.handle.net/10388/12702

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

7. Balaban, Murat. Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni.

Degree: 2011, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/868

► Campylobacter jejuni is a worldwide leading cause of bacterial gastrointestinal disease. The natural habitat of this organism is the gastrointestinal tracts of warm-blooded animals, especially… (more)

▼ Campylobacter jejuni is a worldwide leading cause of bacterial gastrointestinal disease. The natural habitat of this organism is the gastrointestinal tracts of warm-blooded animals, especially poultry, where the bacterium promotoes a harmless commensal colonization. The abundance of C. jejuni in poultry creates a risk for food-borne infections to human populations. Flagellar motility by C. jejuni is required to colonize both human and animal hosts. For motility, C. jejuni produces amphitrichous flagella, resulting in the formation of a single flagellum at both poles. This work explored factors that regulate numerical and spatial parameters for amphitrichious flagellation. Two factors that have been identified to control flagellar placement and numbers in polarly-flagellated bacteria are the FlhF GTPase and the FlhG ATPase. FlhF has been shown to be required for regulation of flagellar gene expression and flagellar placement in some Pseudomonas and Vibrio species. Characterization of FlhF in C. jejuni was accomplished by creating point mutants in C-terminal GTPase domain of FlhF to decrease its GTPase activity. GTPase mutants, unlike mutants that lack FlhF, did not have a significant reduction in sigma54-dependent flagellar gene expression. Instead, a significant proportion of the population produced flagella at lateral sites or produced multiple flagella at a pole, whereas wild-type bacteria produced single polar flagella. Further experiments suggested that FlhF functions downstream of the FlgSR-flagellar export apparatus (FEA) pathway to activate sigma54-dependent flagellar gene expression. Thus, our data suggested that FlhF and its GTPase activity are required for distinct processes in flagellar gene regulation. FlhG has been shown to control flagellar numbers in Pseudomonas and Vibrio species. We examined flhG mutants and confirmed that FlhG regulates flagellar numbers. C. jejuni flhG mutants also demonstrated a minicell phenotype, which is the result of division erroneously occurring at polar regions. Further examination revealed that FlhG and the flagellar base components compose a novel division inhibition system to spatially prevent polar division and encourage septation at the cellular midpoint for symmetrical division. This work greatly extends our understanding of factors that govern spatial and numerical patterns of polar flagellation and has identified an unprecedented system to spatially regulate division in bacteria. Advisors/Committee Members: Hendrixson, David R..

Subjects/Keywords: Campylobacter jejuni; Cell Division; Flagella

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APA (6^th Edition):

Balaban, M. (2011). Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balaban, Murat. “Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed January 19, 2021. http://hdl.handle.net/2152.5/868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balaban, Murat. “Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni.” 2011. Web. 19 Jan 2021.

Vancouver:

Balaban M. Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/2152.5/868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balaban M. Determinants Influencing Polar Flagellar Biosynthesis and Cell Division in Campylobacter jejuni. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

8. Weaver, Morris Eugene. Surface motility phenomena accompanying division of tissue cells in vivo.

Degree: PhD, Zoology, 1958, Oregon State University

URL: http://hdl.handle.net/1957/49947

Subjects/Keywords: Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Weaver, M. E. (1958). Surface motility phenomena accompanying division of tissue cells in vivo. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/49947

Chicago Manual of Style (16^th Edition):

Weaver, Morris Eugene. “Surface motility phenomena accompanying division of tissue cells in vivo.” 1958. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/49947.

MLA Handbook (7^th Edition):

Weaver, Morris Eugene. “Surface motility phenomena accompanying division of tissue cells in vivo.” 1958. Web. 19 Jan 2021.

Vancouver:

Weaver ME. Surface motility phenomena accompanying division of tissue cells in vivo. [Internet] [Doctoral dissertation]. Oregon State University; 1958. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/49947.

Council of Science Editors:

Weaver ME. Surface motility phenomena accompanying division of tissue cells in vivo. [Doctoral Dissertation]. Oregon State University; 1958. Available from: http://hdl.handle.net/1957/49947

Oregon State University

9. Hodge, Frederick Allen. The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis.

Degree: PhD, General Science, 1971, Oregon State University

URL: http://hdl.handle.net/1957/46023

► Cell division in a heat synchronized Tetrahymena pyriformis (GL-I) cell can be delayed if the cell is exposed to X-irradiation (300 kVp, 20 mA, HVL… (more)

▼ Cell division in a heat synchronized Tetrahymena pyriformis (GL-I) cell can be delayed if the cell is exposed to X-irradiation (300 kVp, 20 mA, HVL = 0. 9 mm Cu, 25 R/ sec) prior to a critical time after the end of the synchronizing treatment (EST). At the critical time, the cells undergo a rapid transition from a state of being sensitive to being delayed to one of relative insensitivity. After this time, the coming division is delayed little if at all; that is, there is an "all or none" transition to insensitivity to division delay. However, the second division is delayed, indicating that damage has occurred. The transition point (median critical time) is determined by interpolating the time after EST at which a given radiation exposure splits a population of cells into 50 percent delayed and 50 percent not delayed. The transition point is dose dependent in that the larger the exposure, the later the transition point (31 minutes after EST for 3kR increasing gradually to 51 minutes after EST for 18.5 kR). The division delay response is correlated with a resorption of the developing new mouth as determined from silver stained cells fixed every ten minutes after EST. The length of time for the oral primordium to be resorbed increases as the dose increases and varies with the time of irradiation relative to the transition point. The all or none" nature of the division delay response and the fact that cells irradiated after the transition point seemingly "ignore" the radiation damage until the second generation, together suggest that the damage produced by the irradiation may or may .not trigger a cellular response that results in cell division delay and oral primordium resorption. These responses might suggest that preformed enzymes, possibly from lysosomes, could be involved. Therefore, cells were treated with a known lysosomal stabilizer (hydrocortisone) and a labilizer (vitamin A) continuously before, during, and after exposure to 7.5 kR of X-rays. Cells treated with hydrocortisone- 21- phosphate (5.0 mM) showed an earlier transition point (36.5 minutes after EST) whereas cells treated with vitamin A-acetate (0.2 mM) showed a later transition point (50.5 minutes after EST) relative to the transition point for untreated, irradiated cells (43 minutes after EST). The time of the transition for cells treated with a lysosomal stabilizer (depresses enzyme release) and 7.5 kR corresponds to the transition point for untreated cells irradiated with only 5.0 kR,suggesting less sensitivity. Conversely, the transition point for cells treated with a lysosomal labilizer (enhances enzyme release) and 7.5 kR corresponds to the time of transition for untreated cells exposed to 18.5 kR, suggesting greater sensitivity. However, in all cases, 7.5 kR delayed the divisions about the same amount, whether the agents were present or not, indicating that the agents do not act in a simple dose - modifying manner. The data are consistent with the hypothesis that irradiation produces damage that may or… Advisors/Committee Members: Nachtwey, D. Stuart (advisor).

Subjects/Keywords: Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hodge, F. A. (1971). The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46023

Chicago Manual of Style (16^th Edition):

Hodge, Frederick Allen. “The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis.” 1971. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/46023.

MLA Handbook (7^th Edition):

Hodge, Frederick Allen. “The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis.” 1971. Web. 19 Jan 2021.

Vancouver:

Hodge FA. The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis. [Internet] [Doctoral dissertation]. Oregon State University; 1971. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/46023.

Council of Science Editors:

Hodge FA. The relationship of lysosomal stability to radiation-induced delay of cell division in Tetrahymena pyriformis. [Doctoral Dissertation]. Oregon State University; 1971. Available from: http://hdl.handle.net/1957/46023

Oregon State University

10. Gruber, Helen Elizabeth. X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell.

Degree: PhD, General Science, 1976, Oregon State University

URL: http://hdl.handle.net/1957/45593

Subjects/Keywords: Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gruber, H. E. (1976). X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/45593

Chicago Manual of Style (16^th Edition):

Gruber, Helen Elizabeth. “X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell.” 1976. Doctoral Dissertation, Oregon State University. Accessed January 19, 2021. http://hdl.handle.net/1957/45593.

MLA Handbook (7^th Edition):

Gruber, Helen Elizabeth. “X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell.” 1976. Web. 19 Jan 2021.

Vancouver:

Gruber HE. X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell. [Internet] [Doctoral dissertation]. Oregon State University; 1976. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1957/45593.

Council of Science Editors:

Gruber HE. X-ray and proton induced ultrastructural changes in Chlamydomonas reinhardi, with special reference to the dividing cell. [Doctoral Dissertation]. Oregon State University; 1976. Available from: http://hdl.handle.net/1957/45593

University of Ottawa

11. McLeod, Laura J. Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator .

Degree: 2013, University of Ottawa

URL: http://hdl.handle.net/10393/24284

► The Min system ensures that gram-negative bacteria undergo symmetric cell division. The three Min proteins, MinC, MinD, and MinE, display a dynamic pattern of subcellular… (more)

Subjects/Keywords: Bacterial Cell Division; Min System

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McLeod, L. J. (2013). Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/24284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McLeod, Laura J. “Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator .” 2013. Thesis, University of Ottawa. Accessed January 19, 2021. http://hdl.handle.net/10393/24284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McLeod, Laura J. “Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator .” 2013. Web. 19 Jan 2021.

Vancouver:

McLeod LJ. Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator . [Internet] [Thesis]. University of Ottawa; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10393/24284.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McLeod LJ. Investigation of the Role of Membrane-Induced Conformational Change in the Function of the MinE Bacterial Cell Division Regulator . [Thesis]. University of Ottawa; 2013. Available from: http://hdl.handle.net/10393/24284

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Victoria

12. Macpherson, Sarah. STAT3 regulation of citrate synthase is essential during the initiation of cell growth.

Degree: Department of Biochemistry and Microbiology, 2016, University of Victoria

URL: http://hdl.handle.net/1828/7501

► To exit a non-proliferative state and enter cell division, metazoan cells require external signals to facilitate activation and metabolic reprogramming. As cell growth is required… (more)

▼ To exit a non-proliferative state and enter cell division, metazoan cells require external signals to facilitate activation and metabolic reprogramming. As cell growth is required before cell division, cells redirect their metabolism for de novo synthesis of cell building blocks, including phospholipids for cell membrane construction. How cells coordinate initial signaling events with metabolism is unknown. Lineage-specific factors transmit activating signals via cell surface receptor-ligand interactions. Among these are PI3K/AKT, MAPK/ERK, and JAK/STAT, all of which have been described to contribute to metabolic regulation. In particular, the signal transducer and activator of transcription (STAT) is a transcription factor with broad roles in cell cycle progression and glucose metabolism. Previous data from our laboratory found that one STAT family member, STAT3, was one of the primary signaling pathways activated when transitioning out of a resting state. Inhibition of STAT3 was found to suppress the initiation of cell growth and citrate levels, a main intermediate for fatty acid synthesis, suggesting a connection to cell metabolism. This thesis investigates the role of STAT3 in the regulation of metabolism in cells transitioning from a resting state to a cell growth state. The first chapter of this thesis provides relevant background information on the metabolic and signaling pathways involved in a resting and cell growth state. It also provides data that supports an important role for STAT3 during initial cell growth. The second chapter demonstrates the importance of STAT3 in multiple cell types using a small molecule inhibitor of STAT3, STAT3 knockdown, and knockout experiments. I also establish a potential link between STAT3 and the metabolic enzyme citrate synthase (CS) for the synthesis of citrate. In the third chapter I show that STAT3 transcriptionally regulates CS through two binding sites, CS1 and CS2. Finally, I determine that CS is essential for initial cell growth and that exogenous citrate can rescue the loss in cell growth and proliferation observed in the CS and STAT3 knockdown cells. Together, these findings describe a novel mechanism for initial cell growth whereby signaling and metabolic events are tightly linked to regulate the transition from a resting state to a state of initial cell growth. These results may uncover new strategies to block the initiation of proliferation in human pathological conditions including tumor recurrence and autoimmunity. Advisors/Committee Members: Lum, Julian J. (supervisor).

Subjects/Keywords: Cell division; Cellular signal transduction

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Macpherson, S. (2016). STAT3 regulation of citrate synthase is essential during the initiation of cell growth. (Masters Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/7501

Chicago Manual of Style (16^th Edition):

Macpherson, Sarah. “STAT3 regulation of citrate synthase is essential during the initiation of cell growth.” 2016. Masters Thesis, University of Victoria. Accessed January 19, 2021. http://hdl.handle.net/1828/7501.

MLA Handbook (7^th Edition):

Macpherson, Sarah. “STAT3 regulation of citrate synthase is essential during the initiation of cell growth.” 2016. Web. 19 Jan 2021.

Vancouver:

Macpherson S. STAT3 regulation of citrate synthase is essential during the initiation of cell growth. [Internet] [Masters thesis]. University of Victoria; 2016. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1828/7501.

Council of Science Editors:

Macpherson S. STAT3 regulation of citrate synthase is essential during the initiation of cell growth. [Masters Thesis]. University of Victoria; 2016. Available from: http://hdl.handle.net/1828/7501

Drexel University

13. Hwang, Daniel M. Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis.

Degree: 2013, Drexel University

URL: http://hdl.handle.net/1860/idea:7100

►

In the final event of cell division, the thin membrane bridge connecting dividing cells is severed in an event known as abscission. This completion of… (more)

▼

In the final event of cell division, the thin membrane bridge connecting dividing cells is severed in an event known as abscission. This completion of cell division must be coordinated with the segregation of chromatin into two distinct membrane compartments. When chromosome segregation fails to complete successfully, chromatin becomes trapped at the intracellular bridge and premature severing of this bridge can lead to changes in genetic material. In order to monitor the clearance of chromatin at the intracellular bridge, cells employ the NoCut checkpoint which acts through the Aurora B serine/threonine kinase. Leading up to abscission, two events characterize late cytokinesis: the compaction of microtubules at the intracellular bridge and the transition of spastin/ESCRTIII complex to the secondary ingression site where microtubules are disassembled and the membrane is severed. In this work, using immunofluorescence and live cell techniques, I have characterized the spatiotemporal dynamics of septins at the intracellular bridge with respect to microtubules as well as with spastin and ESCRTIII complex proteins. I have found that septins bind microtubules and form two membrane-associated rings. These two populations of septins display distinct spatiotemporal dynamics, suggesting separate roles in late cytokinesis. The microtubule binding population of septins appear to bundle microtubules and mutational analysis of serine residues, which matched the consensus sequence for Aurora B kinase phosphorylation, lead to changes in microtubule compaction, which were coincident with loss of septin association with microtubules at the intracellular bridge. Treatment with hesperadin, a small molecule inhibitor of Aurora B kinase also produced aberrant accumulation of septins at the intracellular bridge. Characterization of the spatiotemporal dynamics of septins with CHMP4B, CHMP2A and spastin showed that these proteins do not begin to accumulate strongly at the intracellular bridge or transition to the secondary ingression zone until septin rings disappear. I hypothesize that septins aid in the compaction of microtubules at the intracellular bridge and form diffusional barriers, which prevent the abscission machinery from transitioning to the abscission site until the NoCut checkpoint has been satisfied. Overall, I identified septins as potential components of the abscission machinery and regulators of abscission timing

M.S., Biological Sciences – Drexel University, 2013

Advisors/Committee Members: Spiliotis, Elias T., College of Arts and Sciences.

Subjects/Keywords: Life sciences; Cytokinesis; Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hwang, D. M. (2013). Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis. (Thesis). Drexel University. Retrieved from http://hdl.handle.net/1860/idea:7100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hwang, Daniel M. “Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis.” 2013. Thesis, Drexel University. Accessed January 19, 2021. http://hdl.handle.net/1860/idea:7100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hwang, Daniel M. “Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis.” 2013. Web. 19 Jan 2021.

Vancouver:

Hwang DM. Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis. [Internet] [Thesis]. Drexel University; 2013. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1860/idea:7100.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hwang DM. Spatiotemporal Dynamics and Roles of Septins in Late Cytokinesis. [Thesis]. Drexel University; 2013. Available from: http://hdl.handle.net/1860/idea:7100

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

14. Chu, Calvin. Development of a semi-automatic method for cellular migration and division analysis.

Degree: Graduate School of Biomedical Engineering, 2005, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/20543 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:635/SOURCE01?view=true

► Binary image processing algorithms have been implemented in this study tocreate a background subtraction mask for the segmentation of cellular time lapseimages. The complexity in… (more)

Subjects/Keywords: cell division

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APA (6^th Edition):

Chu, C. (2005). Development of a semi-automatic method for cellular migration and division analysis. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/20543 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:635/SOURCE01?view=true

Chicago Manual of Style (16^th Edition):

Chu, Calvin. “Development of a semi-automatic method for cellular migration and division analysis.” 2005. Masters Thesis, University of New South Wales. Accessed January 19, 2021. http://handle.unsw.edu.au/1959.4/20543 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:635/SOURCE01?view=true.

MLA Handbook (7^th Edition):

Chu, Calvin. “Development of a semi-automatic method for cellular migration and division analysis.” 2005. Web. 19 Jan 2021.

Vancouver:

Chu C. Development of a semi-automatic method for cellular migration and division analysis. [Internet] [Masters thesis]. University of New South Wales; 2005. [cited 2021 Jan 19]. Available from: http://handle.unsw.edu.au/1959.4/20543 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:635/SOURCE01?view=true.

Council of Science Editors:

Chu C. Development of a semi-automatic method for cellular migration and division analysis. [Masters Thesis]. University of New South Wales; 2005. Available from: http://handle.unsw.edu.au/1959.4/20543 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:635/SOURCE01?view=true

Michigan State University

15. Pfohl, Ronald John. Experimental initiation of cell division.

Degree: PhD, Department of Zoology, 1967, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:36681

Subjects/Keywords: Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pfohl, R. J. (1967). Experimental initiation of cell division. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:36681

Chicago Manual of Style (16^th Edition):

Pfohl, Ronald John. “Experimental initiation of cell division.” 1967. Doctoral Dissertation, Michigan State University. Accessed January 19, 2021. http://etd.lib.msu.edu/islandora/object/etd:36681.

MLA Handbook (7^th Edition):

Pfohl, Ronald John. “Experimental initiation of cell division.” 1967. Web. 19 Jan 2021.

Vancouver:

Pfohl RJ. Experimental initiation of cell division. [Internet] [Doctoral dissertation]. Michigan State University; 1967. [cited 2021 Jan 19]. Available from: http://etd.lib.msu.edu/islandora/object/etd:36681.

Council of Science Editors:

Pfohl RJ. Experimental initiation of cell division. [Doctoral Dissertation]. Michigan State University; 1967. Available from: http://etd.lib.msu.edu/islandora/object/etd:36681

University of Oxford

16. Yates, Luke Alexander. Structural studies in cell adhesion and division.

Degree: PhD, 2012, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580941

► Cell adhesion is a critical process that allows the organisation and functioning of tissues in three-dimensions. However, the replenishing of cells, via cell division, within… (more)

▼ Cell adhesion is a critical process that allows the organisation and functioning of tissues in three-dimensions. However, the replenishing of cells, via cell division, within tissues is equally important for functioning complex life. Both cell adhesion and division are tightly controlled processes and rely on a complex network of signals that, as yet, are not wholly understood. This Thesis presents a structural analysis of several proteins involved in these processes. In the case of cell adhesion, we have made use of high-throughput (HTP) cloning and expression screening technologies in the Oxford Protein Production Facility (OPPF) for the study of the Kindlin protein family – a recently discovered set of proteins essential for integrin-mediated cell adhesion. As a direct result of the HTP pipeline used we were able to determine the high resolution crystal structure of a single domain, the Pleckstrin Homology Domain, from the isoform Kindlin-1. Deletion of this domain in the full-length protein resulted in impaired integrin activation in vivo. This structure, in combination with molecular dynamics simulation demonstrated that, unlike other well characterised PH domains, the binding of secondary messenger lipids (phosphoinositides) is dictated by a, previously unseen, salt bridge that occludes the putative binding site. Mutation of the salt bridge alters the binding characteristics of this domain in vitro. In addition to the PH domain, we have also studied and biophysically characterised full-length Kindlin-3, a blood cell specific isoform. By optimising baculovirus-infected Sf9 cell expression systems we were able to obtain, for the first time, sufficient quantities of protein for characterisation. Furthermore, by using small-angle X-ray scattering (SAXS) in solution we were able to determine a low resolution solution structure of Kindlin-3, revealing a linear arrangement of its FERM domain - a novel conformation known only otherwise in talin. We characterised the interaction of full-length Kindlin-3 with β-integrin cytoplasmic tails using nuclear magnetic resonance spectroscopy, which confirmed that a direct interaction with a membrane distal NPxY motif occurs, and demonstrated the importance of a preceding Serine/Threonine rich region in peptide binding. In the case of cell division, we have determined the crystal structure of the cell cycle checkpoint control related protein, Cid1, a terminal uridine tranferase from Schizzosaccharomyces pombe, alone and in complex with UTP. Structural and biochemical analysis of Cid1 identified a novel Uridine selection mechanism that is suggested to be conserved in metazoan ZCCHC enzymes involved in let-7 miRNA biogenesis, which are important for proliferation, differentiation and cell fate. We have also demonstrated that Cid1 is an RNA binding protein, a property essential for activity that employs a novel mechanism of RNA binding in the absence of RNA binding motifs. The structural work undertaken in this thesis has focussed on two distinct, but interwoven, aspects of cell…

Subjects/Keywords: 571.6; Cell adhesion; Cell division; Proteins – Analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yates, L. A. (2012). Structural studies in cell adhesion and division. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580941

Chicago Manual of Style (16^th Edition):

Yates, Luke Alexander. “Structural studies in cell adhesion and division.” 2012. Doctoral Dissertation, University of Oxford. Accessed January 19, 2021. http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580941.

MLA Handbook (7^th Edition):

Yates, Luke Alexander. “Structural studies in cell adhesion and division.” 2012. Web. 19 Jan 2021.

Vancouver:

Yates LA. Structural studies in cell adhesion and division. [Internet] [Doctoral dissertation]. University of Oxford; 2012. [cited 2021 Jan 19]. Available from: http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580941.

Council of Science Editors:

Yates LA. Structural studies in cell adhesion and division. [Doctoral Dissertation]. University of Oxford; 2012. Available from: http://ora.ox.ac.uk/objects/uuid:d66f5602-7e49-4042-8ebf-9457e61d56c3 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580941

Washington University in St. Louis

17. Arjes, Heidi Ann. Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria.

Degree: PhD, Biology and Biomedical Sciences: Molecular Genetics and Genomics, 2014, Washington University in St. Louis

URL: https://openscholarship.wustl.edu/etd/1280

► During the cell cycle, a cell must replicate its DNA, segregate the genome and divide into two identical daughter cells with full chromosomes. This… (more)

Subjects/Keywords: cell cycle; cell division; DNA replication; FtsZ

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APA (6^th Edition):

Arjes, H. A. (2014). Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria. (Doctoral Dissertation). Washington University in St. Louis. Retrieved from https://openscholarship.wustl.edu/etd/1280

Chicago Manual of Style (16^th Edition):

Arjes, Heidi Ann. “Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria.” 2014. Doctoral Dissertation, Washington University in St. Louis. Accessed January 19, 2021. https://openscholarship.wustl.edu/etd/1280.

MLA Handbook (7^th Edition):

Arjes, Heidi Ann. “Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria.” 2014. Web. 19 Jan 2021.

Vancouver:

Arjes HA. Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria. [Internet] [Doctoral dissertation]. Washington University in St. Louis; 2014. [cited 2021 Jan 19]. Available from: https://openscholarship.wustl.edu/etd/1280.

Council of Science Editors:

Arjes HA. Failsafe Mechanisms Coordinate Cell Division and the Initiation of DNA Replication in Bacteria. [Doctoral Dissertation]. Washington University in St. Louis; 2014. Available from: https://openscholarship.wustl.edu/etd/1280

University of Dundee

18. Fulcher, Luke James. Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning.

Degree: PhD, 2019, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/f2f8ff45-46a0-4cab-987b-d3c8f73de78d ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788031

► The FAM83 family of proteins are classified based on the presence of a conserved domain of unknown function 1669 (DUF1669) within their N-termini. Although structural… (more)

▼ The FAM83 family of proteins are classified based on the presence of a conserved domain of unknown function 1669 (DUF1669) within their N-termini. Although structural and bioinformatic studies suggest that the DUF1669 domain adopts a phospholipase D-like motif, no phospholipase D activity has yet been demonstrated for FAM83 members experimentally. Thus, the role and function of FAM83 proteins remains elusive. This study provides evidence that FAM83 proteins bind to different isoforms of the casein kinase 1 (CK1) family of Ser/Thr protein kinases and direct them to the distinct cellular sites in which the FAM83 proteins reside. FAM83 members mediate CK1 binding through their conserved DUF1669. In this capacity, FAM83 proteins may act as the A Kinase Anchoring proteins (AKAPs) of the CK1 world, and act analogously to the way AKAPs serve to regulate protein kinase A. Regarded as constitutively-active, promiscuous protein kinases, the regulation of CK1 isoforms is critically important, yet poorly understood. This is particularly crucial when considering the reported participation of CK1 kinases in many, diverse signalling processes, from Wnt signalling to the regulation of Circadian rhythm. Mechanisms regulating CK1 isoforms, through controlling their cellular localisation and therefore substrate accessibility, are thus attractive regulatory targets to facilitate the study into specific CK1 functions, which rely on the recruitment of CK1 isoforms to the relevant cellular sites. As FAM83 proteins have the potential to act in such a manner, investigation was focussed on specific FAM83-CK1 interactions in order to further define this emerging regulatory role of FAM83 members. FAM83D is unique amongst the FAM83 proteins, in that it is the only FAM83 member known to localise to the mitotic spindle. RNA interference approaches targeting FAM83D have been reported to impact chromosome alignment and cause a delay in the timings of cell division. Whilst all FAM83 proteins appear to associate robustly with the CK1 alpha isoforms (CK1α), interestingly, the interaction between FAM83D and CK1α in asynchronous cell extracts was weak. Armed with the knowledge that FAM83D localises to the spindle apparatus in mitosis, a hypothesis that the FAM83D-CK1α interaction might only occur during cell division was developed. This study provides evidence that FAM83D binds and recruits CK1α to the mitotic spindle, and that the FAM83D-CK1α interaction is critical for correct and efficient spindle positioning. Cells devoid of FAM83D, or those harbouring a FAM83D CK1-binding- deficient knockin point mutation, fail to localise CK1α to mitotic spindles, and present with spindle orientation defects, and a concurrent delay in the metaphase-to-anaphase transition. As the spindle position determines the axis of cell division, correctly- orientated spindle positioning is critically important in both development and in the maintenance of healthy adult tissues. When one thinks of mitotic kinases regulating spindle positioning, prominent players such as…

Subjects/Keywords: cell division; mitosis; protein phosphorylation; cell biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fulcher, L. J. (2019). Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/f2f8ff45-46a0-4cab-987b-d3c8f73de78d ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788031

Chicago Manual of Style (16^th Edition):

Fulcher, Luke James. “Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning.” 2019. Doctoral Dissertation, University of Dundee. Accessed January 19, 2021. https://discovery.dundee.ac.uk/en/studentTheses/f2f8ff45-46a0-4cab-987b-d3c8f73de78d ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788031.

MLA Handbook (7^th Edition):

Fulcher, Luke James. “Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning.” 2019. Web. 19 Jan 2021.

Vancouver:

Fulcher LJ. Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning. [Internet] [Doctoral dissertation]. University of Dundee; 2019. [cited 2021 Jan 19]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/f2f8ff45-46a0-4cab-987b-d3c8f73de78d ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788031.

Council of Science Editors:

Fulcher LJ. Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells : FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning. [Doctoral Dissertation]. University of Dundee; 2019. Available from: https://discovery.dundee.ac.uk/en/studentTheses/f2f8ff45-46a0-4cab-987b-d3c8f73de78d ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788031

University of North Texas

19. Malhotra, Divya. brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays.

Degree: 2014, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc799473/

► Subsidiary mother cell (SMC) divisions during stomatal complex formation in Zea mays are asymmetric generating a small subsidiary cell (SC) and a larger epidermal cell.… (more)

Subjects/Keywords: preprophase band; phragmoplast; cell division; subsidiary mother cell divisions; Corn – Genetics.; Cell division.; Mutation (Biology)

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APA (6^th Edition):

Malhotra, D. (2014). brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc799473/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Malhotra, Divya. “brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays.” 2014. Thesis, University of North Texas. Accessed January 19, 2021. https://digital.library.unt.edu/ark:/67531/metadc799473/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Malhotra, Divya. “brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays.” 2014. Web. 19 Jan 2021.

Vancouver:

Malhotra D. brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays. [Internet] [Thesis]. University of North Texas; 2014. [cited 2021 Jan 19]. Available from: https://digital.library.unt.edu/ark:/67531/metadc799473/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Malhotra D. brk1 and dcd1 Act Synergistically in Subsidiary Cell Formation in Zea mays. [Thesis]. University of North Texas; 2014. Available from: https://digital.library.unt.edu/ark:/67531/metadc799473/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Pierre, Anaëlle. Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach.

Degree: Docteur es, Sciences de la vie et de la santé, 2017, Université Paris-Saclay (ComUE)

URL: http://www.theses.fr/2017SACLS038

►

Les cellules positionnent leur plan de division de manière précise et prévisible. En particulier au tout début de l’embryogenèse, la cellule-œuf suit un patron de… (more)

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Les cellules positionnent leur plan de division de manière précise et prévisible. En particulier au tout début de l’embryogenèse, la cellule-œuf suit un patron de clivage extrêmement reproductible, mais néanmoins sensible aux perturbations (manipulation de la forme de la cellule,…), ce qui suggère une plasticité intrinsèque du système. Au cours de ma thèse, je me suis intéressée aux signaux qui déterminent la position des plans de division embryonnaires, et à leur compétition. Dans un premier temps, j’ai développé un modèle pour prédire le positionnement du plan de division à partir de la forme de la cellule, et de la présence éventuelle de polarité maternelle à la membrane ou d’une distribution inhomogène de yolk/organelles dans le cytoplasme. Ce modèle est basé sur les forces de traction exercées par les microtubules des astres interphasiques sur le fuseau mitotique/noyau. Sous l’hypothèse que ces forces dépendent de la longueur des microtubules (dynéine dans le cytoplasme) et sont modulées par la polarité membranaire, il est alors possible de trouver la position d’équilibre du fuseau, qui détermine le futur plan de division. J’ai également reproduit les formes et réarrangements des cellules (blastomères) dans l’embryon après la division, à l’aide d’un programme (The Surface Evolver) qui minimise l’énergie de surface sous différentes contraintes : ici les volumes, tensions de surface et éventuels confinements. En bouclant la génération des formes des blastomères avec la prédiction de leurs divisions (les formes permettent de prédire la division, qui permet de générer les formes des cellules filles, etc…), j’ai pu reproduire de manière quantitative quatre patrons de clivage représentatifs (poisson-zèbre, xenope, oursin, ascidie), jusqu’au stade 8 à 16 cellules, in silico. J’ai également testé le modèle sur des expériences classiques de perturbation dans ces quatre systèmes (Hertwig, Hörstadius, ablation de la polarité,…), et reproduit les observations de la littérature. Cette première partie suggère que ces systèmes sont auto-organisés et que la détermination du plan de division dépend principalement d’un nombre restreint de signaux. Dans un second temps, j’ai cherché à caractériser la compétition entre les signaux de forme et de polarité maternelle chez l’embryon d’oursin, de manière quantitative. Ce projet comprend une part importante d’imagerie 3D (position des centrosomes et division, polarité, forme des blastomères), ainsi que des expériences visant à tester le rôle de la forme/taille des blastomères et de la polarité (séparation des blastomères, microchambres de différentes formes, inhibition de la polarité,…). Les résultats obtenus sont comparés aux prédictions du modèle, cette fois basées sur la forme imagée des blastomères. Ces résultats expérimentaux confirment les hypothèses de l’étude in silico, et permettent d’évaluer la robustesse du système biologique pour affiner le modèle.

Cells position their cleavage plane in a precise and predictable way. In particular, during the early embryogenesis, the…

Advisors/Committee Members: Minc, Nicolas (thesis director).

Subjects/Keywords: Embryogenèse; Division cellulaire; Polarité; Embryogenesis; Cell division; Polarity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pierre, A. (2017). Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach. (Doctoral Dissertation). Université Paris-Saclay (ComUE). Retrieved from http://www.theses.fr/2017SACLS038

Chicago Manual of Style (16^th Edition):

Pierre, Anaëlle. “Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach.” 2017. Doctoral Dissertation, Université Paris-Saclay (ComUE). Accessed January 19, 2021. http://www.theses.fr/2017SACLS038.

MLA Handbook (7^th Edition):

Pierre, Anaëlle. “Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach.” 2017. Web. 19 Jan 2021.

Vancouver:

Pierre A. Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach. [Internet] [Doctoral dissertation]. Université Paris-Saclay (ComUE); 2017. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2017SACLS038.

Council of Science Editors:

Pierre A. Architecture des plans de clivage pendant l'embryogenèse : une approche quantitative : Cleavage pattern architecture in early embryos : a quantitative approach. [Doctoral Dissertation]. Université Paris-Saclay (ComUE); 2017. Available from: http://www.theses.fr/2017SACLS038

21. Bellec, Karen. Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster.

Degree: Docteur es, Biologie cellulaire, biologie du dévelopement, 2018, Rennes 1

URL: http://www.theses.fr/2018REN1B023

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Notch est le récepteur d’une voie de communication intercellulaire, conservée au cours de l’évolution et impliquée dans de nombreux processus développementaux. Chez Drosophila melanogaster, la… (more)

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Notch est le récepteur d’une voie de communication intercellulaire, conservée au cours de l’évolution et impliquée dans de nombreux processus développementaux. Chez Drosophila melanogaster, la spécification et la division des précurseurs des organes sensoriels (SOPs) sont gouvernées par l’activation différentielle de la voie de signalisation Notch. Cette activation est dépendante de l’interaction entre le récepteur Notch et les ligands Delta/Serrate et LAG-2. Cette interaction favorise le clivage protéolytique du récepteur Notch puis la libération et la translocation du domaine intracellulaire dans le noyau de la cellule receveuse du signal. L’activation de Notch est étroitement régulée dans le temps et dans l’espace et est sous le contrôle du trafic intracellulaire. Toutefois, la localisation exacte de l’interaction entre le ligand et le récepteur demeure encore débattue.Précédemment, la protéine Stratum, prédite pour avoir un rôle de facteur d’échange nucléotidique (GEF), fut identifiée comme régulateur de la voie de signalisation Notch. Ici, nous montrons que la perte de Stratum induit des phénotypes Notch associés à une délocalisation du co-facteur de Notch, Sanpodo, au pôle apical des cellules et dans le réseau trans- golgien, avec Notch et Delta. De plus, nous montrons que Rab8 est délocalisée en absence de Stratum et la perte de Rab8 récapitule les phénotypes Notch observés dans le mutant strat. Ensemble, nous résultats indiquent que Stratum et Rab8 régulent la voie de signalisation Notch en contrôlant à la fois le tri et le transport polarisé de Notch, Sanpodo et Delta à la sortie de l’appareil de Golgi.

Notch is the receptor of an evolutionarily conserved intercellular communication pathway, involved in numerous developmental processes. In Drosophila melanogaster, the specification and the division of sensory organ precursors (SOPs) are governed by the differential activation of the Notch signalling pathway. This activation depends on the interaction between the Notch receptor and its ligands Delta/Serrate and LAG-2. This interaction induces the proteolytic cleavage of the Notch receptor, the release and the translocation of the intracellular domain in the nucleus of the signal-receiving cell. The Notch activation is tightly regulated in time and in space and is controlled by intracellular trafficking. However, the exact localisation of the interaction remains debated.Previously, Stratum, predicted to be a guanine exchange factor (GEF), was identified as a regulator of the Notch signalling pathway. Here we show that the loss of Stratum induces Notch phenotypes associated with a mislocalization of the Notch co- factor, Sanpodo, at the apical pole of cells and in the trans-golgi network, with Notch and Delta. Moreover we show that Rab8 is mislocalized in the absence of Stratum and the loss of Rab8 recapitules Notch phenotypes observed in the strat mutant. Together our results indicate that Stratum and Rab8 regulate the Notch signalling pathway by controlling…

Advisors/Committee Members: Le Borgne, Roland (thesis director).

Subjects/Keywords: Signalisation; Polarité; Division Cellulaire; Développement; Signalling; Polarity; Cell Division; Development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bellec, K. (2018). Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster. (Doctoral Dissertation). Rennes 1. Retrieved from http://www.theses.fr/2018REN1B023

Chicago Manual of Style (16^th Edition):

Bellec, Karen. “Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster.” 2018. Doctoral Dissertation, Rennes 1. Accessed January 19, 2021. http://www.theses.fr/2018REN1B023.

MLA Handbook (7^th Edition):

Bellec, Karen. “Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster.” 2018. Web. 19 Jan 2021.

Vancouver:

Bellec K. Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster. [Internet] [Doctoral dissertation]. Rennes 1; 2018. [cited 2021 Jan 19]. Available from: http://www.theses.fr/2018REN1B023.

Council of Science Editors:

Bellec K. Rôle de Stratum dans la régulation de la voie de signalisation Notch au cours de divisions cellulaires asymétriques chez Drosophila melanogaster : Role of Stratum in the regulation of Notch signalling during asymmetric cell divisions in Drosophila melanogaster. [Doctoral Dissertation]. Rennes 1; 2018. Available from: http://www.theses.fr/2018REN1B023

Texas A&M University

22. Guo, Jinbai. Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae.

Degree: PhD, Genetics, 2007, Texas A&M University

URL: http://hdl.handle.net/1969.1/5912

► Cell cycle progression of Saccharomyces cerevisiae cells was monitored in continuous cultures limited for glucose or nitrogen. The G1 cell cycle phase, before initiation of… (more)

▼ Cell cycle progression of Saccharomyces cerevisiae cells was monitored in continuous cultures limited for glucose or nitrogen. The G1 cell cycle phase, before initiation of DNA replication, did not exclusively expand when growth rate decreased. Especially during nitrogen limitation, non-G1 phases expanded almost as much as G1. In addition, cell size remained constant as a function of growth rate. These results contrast with current views that growth requirements are met before initiation of DNA replication, and suggest that distinct nutrient limitations differentially impinge on cell cycle progression. Therefore, multiple mechanisms are hypothesized to regulate the coordination of cell growth and cell division. Genetic interactions were identified between the dose-dependent cell-cycle regulator 2 (DCR2) phosphatase and genes involving in secretion/unfolded protein response pathway, including IRE1, through a genome-wide dominant negative genetic approach. Accumulation of unfolded proteins in the endoplasmic reticulum triggers the unfolded protein response (UPR). How the UPR is downregulated is not well understood. Inositol requirement 1 (IRE1) is an endoplasmic reticulum transmembrane UPR sensor in Saccharomyces cerevisiae. When the UPR is triggered, Ire1p is autophosphorylated, on Ser 840 and Ser 841, inducing the cytosolic endonuclease activity of Ire1p, thereby initiating the splicing and translational de-repression of HAC1 mRNA. Homologous to Atf/Creb1 (Hac1p) activates UPR transcription. We found that that Dcr2p phosphatase functionally and physically interacts with Ire1p. Overexpression of DCR2, but not of a catalytically inactive DCR2 allele, significantly delays HAC1 splicing and sensitizes cells to the UPR. Furthermore, Dcr2p physically interacts in vivo with Ire1p-S840E, S841E, which mimics phosphorylated Ire1p, and Dcr2p dephosphorylates Ire1p in vitro. Our results are consistent with de-phosphorylation of Ire1p being a mechanism for antagonizing UPR signaling. Advisors/Committee Members: Polymenis, Michael (advisor), Aramayo, Rodolfo (committee member), Bryk, Mary (committee member), Pettigrew, Donald W. (committee member).

Subjects/Keywords: Cell division; UPR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guo, J. (2007). Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/5912

Chicago Manual of Style (16^th Edition):

Guo, Jinbai. “Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae.” 2007. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/5912.

MLA Handbook (7^th Edition):

Guo, Jinbai. “Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae.” 2007. Web. 19 Jan 2021.

Vancouver:

Guo J. Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae. [Internet] [Doctoral dissertation]. Texas A&M University; 2007. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/5912.

Council of Science Editors:

Guo J. Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae. [Doctoral Dissertation]. Texas A&M University; 2007. Available from: http://hdl.handle.net/1969.1/5912

Texas A&M University

23. Sung, Min Woo. ARC6 and FtsZ Assembly: Structural and Functional Characterization.

Degree: PhD, Biology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/169542

► Chloroplasts have to divide to maintain their numbers throughout cycles of cell division by binary fission and conduct photosynthesis and fulfill other metabolic functions that… (more)

Subjects/Keywords: ARC6; FtsZ; plastid; Z-ring; cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sung, M. W. (2018). ARC6 and FtsZ Assembly: Structural and Functional Characterization. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/169542

Chicago Manual of Style (16^th Edition):

Sung, Min Woo. “ARC6 and FtsZ Assembly: Structural and Functional Characterization.” 2018. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/169542.

MLA Handbook (7^th Edition):

Sung, Min Woo. “ARC6 and FtsZ Assembly: Structural and Functional Characterization.” 2018. Web. 19 Jan 2021.

Vancouver:

Sung MW. ARC6 and FtsZ Assembly: Structural and Functional Characterization. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/169542.

Council of Science Editors:

Sung MW. ARC6 and FtsZ Assembly: Structural and Functional Characterization. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/169542

Texas A&M University

24. Sung, Min Woo. ARC6 and FtsZ Assembly: Structural and Functional Characterization.

Degree: PhD, Biology, 2018, Texas A&M University

URL: http://hdl.handle.net/1969.1/169548

► Chloroplasts have to divide to maintain their numbers throughout cycles of cell division by binary fission and conduct photosynthesis and fulfill other metabolic functions that… (more)

Subjects/Keywords: ARC6; FtsZ; plastid; Z-ring; cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sung, M. W. (2018). ARC6 and FtsZ Assembly: Structural and Functional Characterization. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/169548

Chicago Manual of Style (16^th Edition):

Sung, Min Woo. “ARC6 and FtsZ Assembly: Structural and Functional Characterization.” 2018. Doctoral Dissertation, Texas A&M University. Accessed January 19, 2021. http://hdl.handle.net/1969.1/169548.

MLA Handbook (7^th Edition):

Sung, Min Woo. “ARC6 and FtsZ Assembly: Structural and Functional Characterization.” 2018. Web. 19 Jan 2021.

Vancouver:

Sung MW. ARC6 and FtsZ Assembly: Structural and Functional Characterization. [Internet] [Doctoral dissertation]. Texas A&M University; 2018. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/1969.1/169548.

Council of Science Editors:

Sung MW. ARC6 and FtsZ Assembly: Structural and Functional Characterization. [Doctoral Dissertation]. Texas A&M University; 2018. Available from: http://hdl.handle.net/1969.1/169548

Victoria University of Wellington

25. Nisa, Shahista Yasmin. ParA: A Novel Target for Anti-Tubercular Drug Discovery.

Degree: 2010, Victoria University of Wellington

URL: http://hdl.handle.net/10063/1364

► Tuberculosis (Tb) continues to be one of the world's greatest challenges in the public health arena. The current treatment for Tb entails a long duration… (more)

▼ Tuberculosis (Tb) continues to be one of the world's greatest challenges in the public health arena. The current treatment for Tb entails a long duration of therapy making adherence to the whole course difficult. This has given rise to drug resistant strains of Mycobacterium tuberculosis which are posing a significant threat to Tb control strategies. To counteract this problem, there is an urgent need to develop alternative anti-tuberculous drugs which target processes that are critical for the growth and/or survival of this microbe. To identify such targets in M. tuberculosis, I used comparative genomics and mutagenesis data to identify conserved essential genes as viable targets for the development of broad-spectrum antibiotics. In addition, I validated the essentiality of three cell division genes in Mycobacterium smegmatis using conditional antisense RNA expression under different culture conditions. Furthermore, I performed high-throughput screens (HTS) using a differential susceptibility assay against one of the validated targets to identify its cognate inhibitor(s). Lastly, I developed a novel biochemical assay of the target to validate the specificity of the inhibitors identified in the HTS and evaluated the potency of the inhibitors against M. tuberculosis. This study identified 261 conserved putative essential genes as broad-spectrum targets. I hypothesized that antisense RNA expression of such genes will lead to its down-regulation and thereby affect the viability of the cells if these genes are essential. I also hypothesized that an essential gene will be required under all culture conditions. One gene, parA, demonstrated that it was essential under various culture conditions. This gene encodes for a protein which contain the conserved Walker A motif thus I theorized that it may posses ATPase activity. The results illustrated that the M. tuberculosis ParA protein possesses ATPase activity. This biochemical activity was used to validate two specific inhibitors of ParA, phenoxybenzamine and octoclothepin, which were identified in the cell-based HTS. Kinetic studies suggest that phenoxybenzamine is a mixed inhibitor while octoclothepin is a competitive inhibitor of ParA. This data is also supported by in silico docking. Both these compounds show low minimum inhibitory concentrations in M. smegmatis under nitrogen starvation conditions. In summary, this thesis illustrates that ParA is a viable target for anti-tubercular drugs. It demonstrates that ParA is an ATPase which has the potential to bind competitive and non-competitive inhibitors that can be exploited to target cell division in M. tuberculosis. Finally, this study presents phenoxybenzamine and octoclothepin as inhibitors of ParA. In conclusion, these compounds can either be developed to increase potency or be used as reference structures to screen for more potent inhibitors of the enzyme. Advisors/Committee Members: O'Toole, Ronan, Kirman, Joanna.

Subjects/Keywords: Mycobacterium tuberculosis; Essential genes; Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nisa, S. Y. (2010). ParA: A Novel Target for Anti-Tubercular Drug Discovery. (Doctoral Dissertation). Victoria University of Wellington. Retrieved from http://hdl.handle.net/10063/1364

Chicago Manual of Style (16^th Edition):

Nisa, Shahista Yasmin. “ParA: A Novel Target for Anti-Tubercular Drug Discovery.” 2010. Doctoral Dissertation, Victoria University of Wellington. Accessed January 19, 2021. http://hdl.handle.net/10063/1364.

MLA Handbook (7^th Edition):

Nisa, Shahista Yasmin. “ParA: A Novel Target for Anti-Tubercular Drug Discovery.” 2010. Web. 19 Jan 2021.

Vancouver:

Nisa SY. ParA: A Novel Target for Anti-Tubercular Drug Discovery. [Internet] [Doctoral dissertation]. Victoria University of Wellington; 2010. [cited 2021 Jan 19]. Available from: http://hdl.handle.net/10063/1364.

Council of Science Editors:

Nisa SY. ParA: A Novel Target for Anti-Tubercular Drug Discovery. [Doctoral Dissertation]. Victoria University of Wellington; 2010. Available from: http://hdl.handle.net/10063/1364

Boston College

26. Szatanek, Tomasz Artur. A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii.

Degree: PhD, Biology, 2010, Boston College

URL: http://dlib.bc.edu/islandora/object/bc-ir:101644

► The length of the tachyzoite cell cycle, in particular G1, is an important virulence factor in Toxoplasma gondii. Cdk and Cyclin activities ultimately control the… (more)

▼ The length of the tachyzoite cell cycle, in particular G1, is an important virulence factor in Toxoplasma gondii. Cdk and Cyclin activities ultimately control the cell cycle; however, the checkpoint control mechanisms diverge from higher eukaryotes and are poorly understood. In order to elucidate these mechanisms, temperature sensitive (ts) mutants were generated by chemical mutagenesis. One of these mutants, called FV-P6, dies within one cell cycle in the G1 phase upon transfer from the permissive (35°C) to the restrictive temperature (40°C). Cosmid complementation identified the gene responsible for this G1 arrest as a `Cactin' ortholog. A single point mutation in this gene that resulted in an amino acid substitution from Tyrosine to Histidine at position 661 in the highly conserved C-terminus was shown to underlay the temperature sensitive effect. Cactin is highly conserved across eukaryotes and plays a role in embryonic development of metazoa although its mechanism of action is poorly understood. In agreement with the predicted nuclear localization signal in the N-terminus, expression of a fluorescent reporter gene fusion resulted in nuclear localization. Genome-wide expression profiling analysis of mutant and wild type at the permissive and restrictive temperatures confirmed the G1 arrest and furthermore demonstrated up-regulation of bradyzoite and Toxoplasma cat life cycle stage genes, hinting at TgCactin's role as a repressor. Since DNA binding domains or enzymatic domains are absent in TgCactin, TgCactin must act in a complex. Native blue gel electrophoresis demonstrated that TgCactin is present in large complexes of 720 and 800 kDa. A yeast two-hybrid screen (YTH) identified 40 potential TgCactin-interacting proteins of which 10 were selected for further validation. Eight out of these ten candidates are involved in DNA/RNA processes pertaining to transcription and translation, respectively. One-on-one YTH interactions between mutated and N-terminal deletion mutants of TgCactin and the above 10 interactors were abolished except for a single RNA helicase. Studies in Toxoplasma of four of these interactors demonstrated that only the RNA helicase localized to the nucleus; however, co-immunoprecipitation experiments to demonstrate that this protein is present in a complex with TgCactin were inconclusive. Furthermore, TgCactin self interactions identified domains necessary for TgCactin-TgCactin binding. Taken together, these findings indicate that TgCactin likely functions as a repressor of gene expression, possibly through an epigenetic mechanism reminiscent of an RNA/DNA helicase- based system in plants. Advisors/Committee Members: Marc Jan Gubbels (Thesis advisor), Thomas Chiles (Thesis advisor).

Subjects/Keywords: Cactin; cell division; G1 phase; Toxoplasma gondii

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Szatanek, T. A. (2010). A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101644

Chicago Manual of Style (16^th Edition):

Szatanek, Tomasz Artur. “A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii.” 2010. Doctoral Dissertation, Boston College. Accessed January 19, 2021. http://dlib.bc.edu/islandora/object/bc-ir:101644.

MLA Handbook (7^th Edition):

Szatanek, Tomasz Artur. “A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii.” 2010. Web. 19 Jan 2021.

Vancouver:

Szatanek TA. A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii. [Internet] [Doctoral dissertation]. Boston College; 2010. [cited 2021 Jan 19]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101644.

Council of Science Editors:

Szatanek TA. A Temperature Sensitive Mutation in Cactin Causes a G1 Phase Arrest in Toxoplasma gondii. [Doctoral Dissertation]. Boston College; 2010. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101644

Columbia University

27. Pernice, Wolfgang Maximilian. Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae.

Degree: 2016, Columbia University

URL: https://doi.org/10.7916/D8N58MP3

► Both an intuitive observation and maybe the most mysterious process of biology, aging describes the progressive deterioration of cellular functions with time. Asymmetric cell divisions… (more)

Subjects/Keywords: Cytology; Cell division; Mitochondria; Saccharomyces cerevisiae; Aging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pernice, W. M. (2016). Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8N58MP3

Chicago Manual of Style (16^th Edition):

Pernice, Wolfgang Maximilian. “Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae.” 2016. Doctoral Dissertation, Columbia University. Accessed January 19, 2021. https://doi.org/10.7916/D8N58MP3.

MLA Handbook (7^th Edition):

Pernice, Wolfgang Maximilian. “Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae.” 2016. Web. 19 Jan 2021.

Vancouver:

Pernice WM. Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae. [Internet] [Doctoral dissertation]. Columbia University; 2016. [cited 2021 Jan 19]. Available from: https://doi.org/10.7916/D8N58MP3.

Council of Science Editors:

Pernice WM. Asymmetric Mitochondrial Inheritance and Retention in the Regulation of Aging in S. cerevisiae. [Doctoral Dissertation]. Columbia University; 2016. Available from: https://doi.org/10.7916/D8N58MP3

Columbia University

28. Thiyagarajan, Sathish. Mechanical Regulation in Cell Division and in Neurotransmitter Release.

Degree: 2018, Columbia University

URL: https://doi.org/10.7916/D80V9QPQ

► During their lifecycle, cells must produce forces which play important roles in several subcellular processes. Force-producing components are organized into macromolecular assemblies of proteins that… (more)

▼ During their lifecycle, cells must produce forces which play important roles in several subcellular processes. Force-producing components are organized into macromolecular assemblies of proteins that are often dynamic, and are constructed or disassembled in response to various signals. The forces themselves may directly be involved in subcellular mechanics, or they may influence mechanosensing proteins either within or outside these structures. These proteins play different roles: they may ensure the stability of the force-producing structure, or they may send signals to a coupled process. The generation and sensing of subcellular forces is an active research topic, and this thesis focusses on the roles of these forces in two key areas: cell division and neurotransmitter release. The first part of the thesis deals with the effect of force on cell wall growth regulation during division in the fission yeast Schizosaccharomyces pombe, a cigar-shaped, unicellular organism. During cytokinesis, the last stage of cell division in which the cell physically divides into two, a tense cytokinetic ring anchored to the cellular membrane assembles and constricts, accompanied by the inward centripetal growth of new cell wall, called septum, in the wake of the inward-moving membrane. The contour of the septum hole maintains its circularity as it reduces in size—an indication of regulated growth. To characterize the cell wall growth process, we performed image analysis on contours of the leading edge of the septum obtained via fluorescence microscopy in the labs of our collaborators. We quantified the deviations from circularity using the edge roughness. The roughness was spatially correlated, suggestive of regulated growth. We hypothesized that the cell wall growers are mechanosensitive and respond to the force exerted by the ring. A mathematical model based on this hypothesis then showed that this leads to corrections of roughness in a curvature-dependent fashion. Thus, one of the roles of ring tension is to communicate with the mechanosensitive septum growth processes and coordinate growth to ensure the daughter cells have a functional cell wall. The second part of the thesis deals with how ring tension is produced and sustained, using experimentally measured ultrastructure of the cytokinetic ring itself. Recent super-resolution experiments have revealed that several key proteins of the fission yeast constricting ring are organized into membrane-anchored complexes called nodes. The force producing protein myosin-II in these nodes exerts pulling forces on polymeric actin filaments that are synthesized from polymerizers residing in the nodes. How these forces are marshalled to generate ring tension, and how such an organization maintains its stability is unclear. Using a mathematical model with coarse-grained representations of actin and myosin, we showed that such a node-based organization reproduces previously measured ring tension values. The model explains the origin of experimentally observed bidirectional motion…

Subjects/Keywords: Physics; Biophysics; Cytology; Cell division; Neurotransmitters

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Thiyagarajan, S. (2018). Mechanical Regulation in Cell Division and in Neurotransmitter Release. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D80V9QPQ

Chicago Manual of Style (16^th Edition):

Thiyagarajan, Sathish. “Mechanical Regulation in Cell Division and in Neurotransmitter Release.” 2018. Doctoral Dissertation, Columbia University. Accessed January 19, 2021. https://doi.org/10.7916/D80V9QPQ.

MLA Handbook (7^th Edition):

Thiyagarajan, Sathish. “Mechanical Regulation in Cell Division and in Neurotransmitter Release.” 2018. Web. 19 Jan 2021.

Vancouver:

Thiyagarajan S. Mechanical Regulation in Cell Division and in Neurotransmitter Release. [Internet] [Doctoral dissertation]. Columbia University; 2018. [cited 2021 Jan 19]. Available from: https://doi.org/10.7916/D80V9QPQ.

Council of Science Editors:

Thiyagarajan S. Mechanical Regulation in Cell Division and in Neurotransmitter Release. [Doctoral Dissertation]. Columbia University; 2018. Available from: https://doi.org/10.7916/D80V9QPQ

Columbia University

29. Yen, Bonnie. Asymmetric Cell Division in the Generation of Immunity and Tolerance.

Degree: 2018, Columbia University

URL: https://doi.org/10.7916/D8NP3GB7

► The immune system relies on the collaboration of heterogeneous cell types to respond to infection, develop immunological memory, and to maintain immunological tolerance. In response… (more)

Subjects/Keywords: Immunology; Cell division; Immunological tolerance; Molecular biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yen, B. (2018). Asymmetric Cell Division in the Generation of Immunity and Tolerance. (Doctoral Dissertation). Columbia University. Retrieved from https://doi.org/10.7916/D8NP3GB7

Chicago Manual of Style (16^th Edition):

Yen, Bonnie. “Asymmetric Cell Division in the Generation of Immunity and Tolerance.” 2018. Doctoral Dissertation, Columbia University. Accessed January 19, 2021. https://doi.org/10.7916/D8NP3GB7.

MLA Handbook (7^th Edition):

Yen, Bonnie. “Asymmetric Cell Division in the Generation of Immunity and Tolerance.” 2018. Web. 19 Jan 2021.

Vancouver:

Yen B. Asymmetric Cell Division in the Generation of Immunity and Tolerance. [Internet] [Doctoral dissertation]. Columbia University; 2018. [cited 2021 Jan 19]. Available from: https://doi.org/10.7916/D8NP3GB7.

Council of Science Editors:

Yen B. Asymmetric Cell Division in the Generation of Immunity and Tolerance. [Doctoral Dissertation]. Columbia University; 2018. Available from: https://doi.org/10.7916/D8NP3GB7

Brunel University

30. Sharma, Chetana Devi. Telomere maintenance using cell lines from Dyskeratosis Congenita patients.

Degree: PhD, 2016, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/12729 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687649

► Cells exposed to DNA damaging agents activate a network of mechanisms called DNA damage response, including telomere length regulation. Telomeres are specialized structures that protect… (more)

Subjects/Keywords: 572.8; Telomere; Shelterin; Dyskerin; Chromosomes; Cell division

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sharma, C. D. (2016). Telomere maintenance using cell lines from Dyskeratosis Congenita patients. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/12729 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687649

Chicago Manual of Style (16^th Edition):

Sharma, Chetana Devi. “Telomere maintenance using cell lines from Dyskeratosis Congenita patients.” 2016. Doctoral Dissertation, Brunel University. Accessed January 19, 2021. http://bura.brunel.ac.uk/handle/2438/12729 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687649.

MLA Handbook (7^th Edition):

Sharma, Chetana Devi. “Telomere maintenance using cell lines from Dyskeratosis Congenita patients.” 2016. Web. 19 Jan 2021.

Vancouver:

Sharma CD. Telomere maintenance using cell lines from Dyskeratosis Congenita patients. [Internet] [Doctoral dissertation]. Brunel University; 2016. [cited 2021 Jan 19]. Available from: http://bura.brunel.ac.uk/handle/2438/12729 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687649.

Council of Science Editors:

Sharma CD. Telomere maintenance using cell lines from Dyskeratosis Congenita patients. [Doctoral Dissertation]. Brunel University; 2016. Available from: http://bura.brunel.ac.uk/handle/2438/12729 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687649

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