subject:(carotenoid)

Royal Holloway, University of London

1. Klompmaker, Martin. Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis.

Degree: PhD, 2018, Royal Holloway, University of London

URL: https://pure.royalholloway.ac.uk/portal/en/publications/insights-into-carotenoid-sequestration-mechanisms-in-tomato-fruit-solanum-lycopersicum-and-the-effect-of-ectopic-expression-approaches-on-carotenoid-biosynthesis(01849284-1c1f-4910-a181-01af1523c398).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792791

► Carotenoids are involved in a wide range of plant processes varying from development to defence, pollination and seed dispersal. The consumption benefits of carotenoids for… (more)

▼ Carotenoids are involved in a wide range of plant processes varying from development to defence, pollination and seed dispersal. The consumption benefits of carotenoids for human health and nutrition have led to development of an interest in the industrial application of carotenoids in food, feed and cosmetics. Tomato (Solanum lycopersicum) is the model plant for carotenoids related studies with its fruits which contain high levels of carotenoids. Carotenoid sequestration is an important process linked to carotenoid biosynthesis which requires further elucidation. A selection of tomato lines perturbed in carotenoid biosynthesis was studied for their differences in sequestration mechanisms. Premature accumulation of carotenes in a tomato line constitutively expressing PSY1 led to the differentiation of chloroplasts to chromoplast in immature fruit to create a higher capacity for carotenoid accumulation. Tomato lines rr, ogC and tan, knock out lines for PSY1, LCY-B and CRT-ISO respectively, demonstrated different distributions between plastoglobules and crystalline membrane structures associated with cis-carotenes and trans-carotenes. The role of carotenoid accumulation and changes in carotenoid profiles suggests the plastid can adapt to changes in carotenoid content through plastid differentiation and preferential sequestration. Ectopic expression of the MEP and bacterial carotenoid (crtE, crtB, crtI) pathway in the cytosol has hereby been attempted to enhance carotenoid content as an alternative to metabolic engineering of endogenous key enzymes. The isoprenoid biosynthesis clusters are spread over various cellular compartments in plants. A modular cloning system (GB Cloning) was used for the assembly of vectors with the genes of the MEP and bacterial carotenoid pathway in single or multi gene combinations. The ectopic expression of crtB led to the accumulation of phytoene (14-fold), phytofluene and lycopene, and increased levels of lutein and β-carotene. The impact of the enzyme on pathway intermediates beyond its function suggest a signalling cascade based on cytosolic apocarotenoids derived from the CRTB activity.

Subjects/Keywords: BIOCHEMISTRY; CAROTENOID

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APA (6^th Edition):

Klompmaker, M. (2018). Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis. (Doctoral Dissertation). Royal Holloway, University of London. Retrieved from https://pure.royalholloway.ac.uk/portal/en/publications/insights-into-carotenoid-sequestration-mechanisms-in-tomato-fruit-solanum-lycopersicum-and-the-effect-of-ectopic-expression-approaches-on-carotenoid-biosynthesis(01849284-1c1f-4910-a181-01af1523c398).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792791

Chicago Manual of Style (16^th Edition):

Klompmaker, Martin. “Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis.” 2018. Doctoral Dissertation, Royal Holloway, University of London. Accessed January 21, 2021. https://pure.royalholloway.ac.uk/portal/en/publications/insights-into-carotenoid-sequestration-mechanisms-in-tomato-fruit-solanum-lycopersicum-and-the-effect-of-ectopic-expression-approaches-on-carotenoid-biosynthesis(01849284-1c1f-4910-a181-01af1523c398).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792791.

MLA Handbook (7^th Edition):

Klompmaker, Martin. “Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis.” 2018. Web. 21 Jan 2021.

Vancouver:

Klompmaker M. Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis. [Internet] [Doctoral dissertation]. Royal Holloway, University of London; 2018. [cited 2021 Jan 21]. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/insights-into-carotenoid-sequestration-mechanisms-in-tomato-fruit-solanum-lycopersicum-and-the-effect-of-ectopic-expression-approaches-on-carotenoid-biosynthesis(01849284-1c1f-4910-a181-01af1523c398).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792791.

Council of Science Editors:

Klompmaker M. Insights into carotenoid sequestration mechanisms in tomato fruit (Solanum lycopersicum) and the effect of ectopic expression approaches on carotenoid biosynthesis. [Doctoral Dissertation]. Royal Holloway, University of London; 2018. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/insights-into-carotenoid-sequestration-mechanisms-in-tomato-fruit-solanum-lycopersicum-and-the-effect-of-ectopic-expression-approaches-on-carotenoid-biosynthesis(01849284-1c1f-4910-a181-01af1523c398).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.792791

Université de Neuchâtel

2. Rottet, Sarah. Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence.

Degree: 2016, Université de Neuchâtel

URL: http://doc.rero.ch/record/288913

► La photosynthèse est le processus clé se déroulant au sein du chloroplaste. La machinerie photosynthétique est intégrée à une matrice très dynamique, la membrane des… (more)

▼ La photosynthèse est le processus clé se déroulant au sein du chloroplaste. La machinerie photosynthétique est intégrée à une matrice très dynamique, la membrane des thylakoïdes. Les galactolipides, les principaux lipides polaires de cette membrane, lui confèrent la propriété de bicouche alors que les lipides neutres tels que les prényllipides et les caroténoïdes contribuent à d’essentielles fonctions telles que le transport d’électrons et la photoprotection. Malgré de nombreuses études, la biogénèse et la dynamique des thylakoïdes restent irrésolus. Les plastoglobules (PG), des gouttelettes lipidiques associées aux thylakoïdes, participent activement aux fonctions des thylakoïdes. Sous un environnement changeant et lors des différents stades de développement, le recrutement d’enzymes spécifiques permet aux PG de participer à la synthèse, la réparation et l’élimination des métabolites. Dans le premier chapitre de cette thèse, nous passons en revue les PG en tant que microdomaines des thylakoïdes et discutons leur implication dans le remodelage lipidique lors de stress et lors de la conversion d’un type de plaste à l’autre. Le chapitre II est dédié à la caractérisation de NAP13 (ABCI10) et -14 (ABCI11). Ces deux protéines appartiennent à la grande famille des ABC. Chaque membre de la sous-famille ABCI présente un seul des domaines typiques des transporteurs ABC. NAP13 et -14 codent deux domaines prédits pour la liaison des nucléotides. En se basant sur de précédents résultats (Shimoni-Shor et al. 2010), notre première hypothèse impliquait NAP14, éventuellement avec NAP13, dans un transport de lipides entre les PG et les thylakoïdes. Cependant, cette thèse démontre que NAP13 est extrinsèquement associée à l’enveloppe interne du chloroplaste, alors que NAP14 cofractionne avec les thylakoïdes. Les mutants nap13 et -14 ont un phénotype albinos, ce qui souligne une fonction essentielle au sein du chloroplaste. Notons que nap13 et -14 ont un profile lipidique similaire, bien que distinct de trois autres mutants albinos. L’observation la plus pertinente étant leur taux réduit de phosphatidyléthanolamine 16:0/18:3. Les similarités partagées par nap13 et -14 suggèrent que les protéines correspondantes contribuent à la même voie métabolique. D’après nos résultats, il s’agirait d’un transport de lipides au niveau de l’enveloppe. Dans le chapitre III, nous décrivons la caractérisation de CCD4, une enzyme qui clive les caroténoïdes, et son association physique et fonctionnelle avec les PG. La fusion d’une protéine fluorescente avec CCD4 a donné lieu à un signal ponctué dans les chloroplastes typique d’une localisation aux PG in vivo. Pour élucider la fonction de CCD4, une étude de lipidomique comparative de mutants ccd4 et de plantes sauvages a été effectuée sous diverses conditions. Les résultats indiquent que CCD4 est impliquée dans la dégradation du β-carotène et de la lutéine lors de la sénescence des feuilles. Pour approfondir, nous avons conçu… Advisors/Committee Members: Felix (Dir.).

Subjects/Keywords: carotenoid cleavage

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rottet, S. (2016). Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence. (Thesis). Université de Neuchâtel. Retrieved from http://doc.rero.ch/record/288913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Rottet, Sarah. “Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence.” 2016. Thesis, Université de Neuchâtel. Accessed January 21, 2021. http://doc.rero.ch/record/288913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Rottet, Sarah. “Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence.” 2016. Web. 21 Jan 2021.

Vancouver:

Rottet S. Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence. [Internet] [Thesis]. Université de Neuchâtel; 2016. [cited 2021 Jan 21]. Available from: http://doc.rero.ch/record/288913.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Rottet S. Investigations of candidate plastoglobule proteins: characterization of chloroplast NON-INTRINSIC ABC PROTEINS 13 and -14 in "Arabidopsis thaliana" & Constitutive expression of CAROTENOID CLEAVAGE DIOXYGENASE 4 leads to a reduced level of photosynthesis-related carotenoids during senescence. [Thesis]. Université de Neuchâtel; 2016. Available from: http://doc.rero.ch/record/288913

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

3. Elsen, Amy. Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes.

Degree: MS, 0191, 2012, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/31935

► Carotenoids are very abundant in nature and their consumption has numerous positive health outcomes. There are two known enzymes that are responsible for cleaving carotenoids… (more)

Subjects/Keywords: carotenoid-15,15'-oxygenase; carotenoid 9'10'-monoxygenase; Lycopene

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APA (6^th Edition):

Elsen, A. (2012). Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/31935

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Elsen, Amy. “Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes.” 2012. Thesis, University of Illinois – Urbana-Champaign. Accessed January 21, 2021. http://hdl.handle.net/2142/31935.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Elsen, Amy. “Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes.” 2012. Web. 21 Jan 2021.

Vancouver:

Elsen A. Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2012. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2142/31935.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Elsen A. Genotype effects impact lipids and organ weights in female mice lacking carotenoid cleavage enzymes. [Thesis]. University of Illinois – Urbana-Champaign; 2012. Available from: http://hdl.handle.net/2142/31935

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

4. Mcquinn, Ryan. Carotenoid Biosynthesis And Regulation: More Than Meets The Eye.

Degree: PhD, Plant Biology, 2015, Cornell University

URL: http://hdl.handle.net/1813/40708

► Carotenoids are antioxidants synthesized almost exclusively in plants, providing pigmentation in flowers and fruits for the attraction of pollinators and seed dispersing organisms. While carotenoids… (more)

▼ Carotenoids are antioxidants synthesized almost exclusively in plants, providing pigmentation in flowers and fruits for the attraction of pollinators and seed dispersing organisms. While carotenoids are essential throughout plant development, they are also extremely important in human diets providing necessary nutrition and aiding in the prevention of various cancers, age-related diseases and macular degeneration. The elevated carotenoid content in ripe tomato fruit make them ideal sources of dietary carotenoids and a model system for discovery of how carotenoid accumulation is regulated. Recent efforts to improve nutritional quality of various crops (e.g. maize, rice and potato) by increasing carotenoid biosynthesis have proven useful, but are still limited compared to the high carotenoid content achieved in ripe tomato fruit. Breeding and transgenic efforts to enhance carotenoid content in tomato fruit have provided evidence of feedback mechanisms, which must be understood and overcome to substantially impact fruit carotenoid content. Using tomato as a model system we explored feedback mechanisms limiting the augmentation of carotenoid biosynthesis in ripening fruit with the goal of developing genetic strategies facilitating improvement of nutritional quality of tomato and other fruit and vegetable crops. Recent reports in plants, as well as animals, provide evidence that carotenoid cleavage products (apocarotenoids) play important roles as signaling molecules and logical candidates for roles in the previously mentioned feedback mechanisms. My thesis project focused on building a more complete understanding of signaling involved in ripening-associated carotenogenesis through the achievement of three specific goals: 1. Functionally characterize ZDS, a carotenoid desaturase encoding gene not yet defined by mutation, via the use of transgenic technologies; 2. develop a strategy to circumvent previously observed negative feedback regulation via overexpression of a heterologous phytoene desaturase; 3. Explore the potential for the identification of unique carotenoid derived signals through QTL mapping of ciscarotenoid associated loci in a little explored Solanum habrochaites introgression population. Upon completion, this work will provide a "tool box" for those interested in carotenoid biology and crop improvement to employ in their search for novel carotenoid catabolism pathways essential for carotenoid-derived signal production and eventual targeted manipulation of carotenoid profiles and accumulation. Advisors/Committee Members: Giovannoni,James J. (chair), Gan,Susheng (committee member), Rose,Jocelyn (committee member).

Subjects/Keywords: Carotenoid; Biotechnology; Regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mcquinn, R. (2015). Carotenoid Biosynthesis And Regulation: More Than Meets The Eye. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/40708

Chicago Manual of Style (16^th Edition):

Mcquinn, Ryan. “Carotenoid Biosynthesis And Regulation: More Than Meets The Eye.” 2015. Doctoral Dissertation, Cornell University. Accessed January 21, 2021. http://hdl.handle.net/1813/40708.

MLA Handbook (7^th Edition):

Mcquinn, Ryan. “Carotenoid Biosynthesis And Regulation: More Than Meets The Eye.” 2015. Web. 21 Jan 2021.

Vancouver:

Mcquinn R. Carotenoid Biosynthesis And Regulation: More Than Meets The Eye. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1813/40708.

Council of Science Editors:

Mcquinn R. Carotenoid Biosynthesis And Regulation: More Than Meets The Eye. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/40708

North Carolina State University

5. Gauger, Kelly Ann. Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads.

Degree: MS, Chemistry, 2010, North Carolina State University

URL: http://www.lib.ncsu.edu/resolver/1840.16/6303

► The first part of this thesis discusses the synthesis of chlorins bearing electron donating groups in the 3-position. A 3-dimethylaminochlorin ZnC-(NMe2)3M10 was synthesized in 49%… (more)

Subjects/Keywords: porphyrin; carotenoid; chlorin

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APA (6^th Edition):

Gauger, K. A. (2010). Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/6303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gauger, Kelly Ann. “Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads.” 2010. Thesis, North Carolina State University. Accessed January 21, 2021. http://www.lib.ncsu.edu/resolver/1840.16/6303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gauger, Kelly Ann. “Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads.” 2010. Web. 21 Jan 2021.

Vancouver:

Gauger KA. Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads. [Internet] [Thesis]. North Carolina State University; 2010. [cited 2021 Jan 21]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/6303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gauger KA. Synthesis of Novel Chlorins and Carotenoid-Porphyrin Dyads. [Thesis]. North Carolina State University; 2010. Available from: http://www.lib.ncsu.edu/resolver/1840.16/6303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

6. Haley, Hannah M. Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.

Degree: PhD, Chemistry, 2018, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/101323

► The peroxidation of polyunsaturated fatty acids is a hallmark of many human disorders, yet is has often remained ambiguous whether lipid peroxidation plays a causative… (more)

▼ The peroxidation of polyunsaturated fatty acids is a hallmark of many human disorders, yet is has often remained ambiguous whether lipid peroxidation plays a causative and therefore addressable role in the onset or pathogenesis of a disease. Small molecule inhibitors of this oxidative process in theory possess the capacity to help clarify this ambiguity, but many of the currently available compounds have important limitations. We therefore proposed that context-specific selective pressures may have promoted the evolution of natural products with potent antilipoperoxidant activity in microorganisms that thrive in environments of extreme oxidative stress. Enabled by a modular building-block synthetic platform, the structurally atypical carotenoid peridinin was found to be a potent inhibitor of non-enzymatic bilayer lipid peroxidation. With the goal of maximizing the utility of peridinin as a small molecule probe of the role of lipid peroxidation in pathogenesis, the mechanistic underpinnings of its antilipoperoxidant activity were investigated. Solid-state NMR experiments with a site-selectively 13C-labeled peridinin isotopologue revealed that the potency is linked to a high effective molarity within lipid bilayers. In contrast to the primarily extramembranous localization of the less effective antilipoperoxidant astaxanthin, peridinin is completely embedded within and physically spans the hydrophobic core of lipid membranes. This antilipoperoxidant probe was leveraged in human primary endothelial cell and mouse model experiments to interrogate the contribution of membrane oxidation towards the pathogenesis of atherosclerosis and asthma. The peridinin-mediated mitigation of bilayer lipid peroxidation was shown to attenuate monocyte-endothelial cell adhesion, a key step in atherogenesis. Utilizing mouse models of acute asthma, we found that lipid peroxidation is alternatively not a key driver of the asthmatic phenotype. These results suggest that peridinin may have broad potential as a chemical probe to better understand the role of bilayer lipid peroxidation in human disease. Advisors/Committee Members: Burke, Martin D. (advisor), Burke, Martin D. (Committee Chair), Denmark, Scott E. (committee member), Imlay, James A. (committee member), van der Donk, Wilfred A. (committee member).

Subjects/Keywords: carotenoid; lipid oxidation; atherosclerosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haley, H. M. (2018). Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101323

Chicago Manual of Style (16^th Edition):

Haley, Hannah M. “Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.” 2018. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 21, 2021. http://hdl.handle.net/2142/101323.

MLA Handbook (7^th Edition):

Haley, Hannah M. “Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.” 2018. Web. 21 Jan 2021.

Vancouver:

Haley HM. Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2142/101323.

Council of Science Editors:

Haley HM. Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/101323

Royal Holloway, University of London

7. Nogueira, Marilise. Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration.

Degree: PhD, 2013, Royal Holloway, University of London

URL: https://pure.royalholloway.ac.uk/portal/en/publications/optimisation-of-highvalue-isoprenoid-production-in-plants(520a6d68-0716-4d20-88a2-923e4104a53f).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603452

► Carotenoids and ketocarotenoids are isoprenoid molecules, which represent one of the most widespread classes of natural pigments, found in animals, plants and microorganisms. Moreover, they… (more)

▼ Carotenoids and ketocarotenoids are isoprenoid molecules, which represent one of the most widespread classes of natural pigments, found in animals, plants and microorganisms. Moreover, they have valuable antioxidant properties. Their health benefits and colorant aspects have led to attempts to elevate their level in foodstuffs. In the present study, several metabolic engineering strategies were tested in order to enhance the levels of high-value carotenoid and ketocarotenoid compounds, such as lycopene, -carotene, canthaxanthin and astaxanthin, in tomato and tobacco plants. Biosynthetic bacterial pathway genes have been overexpressed independently (GGPP synthase (CrtE), phytoene synthase (CrtB) and phytoene desaturase (CrtI)) and in combination (CrtE+B, CrtE+I, CrtB+I and -carotene hydroxylase and ketolase (CrtZ+W)), with different promoters (for CrtB, CrtI and CrtB+I) or in association with transcription factors (Phytochrome-interacting factor 5 (CrtZ+W+PIF5) and Arabidopsis Response Regulator 14 (CrtZ+W+ARR14)). The effects of these different strategies on the plant metabolism and especially on carotenoid formation, sequestration and the activation of regulation mechanisms were studied. The combination of the two genes CrtB and CrtI, in their hemizygous state, had a synergistic effect on the production of carotenoids and the expression of CrtZ+W+ARR14 increased the levels of ketocarotenoids in the plants. The important features for the design of metabolic engineering strategies were highlighted. Moreover, regulatory mechanisms that operate across multiple levels of cellular regulation, including transcription, protein localisation, metabolite levels, cell or tissue type, and organelle/sub-organelle structure and organisation were revealed. It was demonstrated how changes to chromoplast and sub-chromoplast structures, such as crystal formation, plastoglobule and membrane composition/structures can arise in response to changes in metabolites. A new carotenoid regulation mechanism at the sub-organellar level was discovered and a schematic model was proposed.

Subjects/Keywords: 572.59; carotenoid; ketocarotenoid; sequestration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Nogueira, M. (2013). Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration. (Doctoral Dissertation). Royal Holloway, University of London. Retrieved from https://pure.royalholloway.ac.uk/portal/en/publications/optimisation-of-highvalue-isoprenoid-production-in-plants(520a6d68-0716-4d20-88a2-923e4104a53f).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603452

Chicago Manual of Style (16^th Edition):

Nogueira, Marilise. “Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration.” 2013. Doctoral Dissertation, Royal Holloway, University of London. Accessed January 21, 2021. https://pure.royalholloway.ac.uk/portal/en/publications/optimisation-of-highvalue-isoprenoid-production-in-plants(520a6d68-0716-4d20-88a2-923e4104a53f).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603452.

MLA Handbook (7^th Edition):

Nogueira, Marilise. “Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration.” 2013. Web. 21 Jan 2021.

Vancouver:

Nogueira M. Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration. [Internet] [Doctoral dissertation]. Royal Holloway, University of London; 2013. [cited 2021 Jan 21]. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/optimisation-of-highvalue-isoprenoid-production-in-plants(520a6d68-0716-4d20-88a2-923e4104a53f).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603452.

Council of Science Editors:

Nogueira M. Optimisation of high-value isoprenoid production in plants : potential strategies and insight into carotenoid sequestration. [Doctoral Dissertation]. Royal Holloway, University of London; 2013. Available from: https://pure.royalholloway.ac.uk/portal/en/publications/optimisation-of-highvalue-isoprenoid-production-in-plants(520a6d68-0716-4d20-88a2-923e4104a53f).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603452

University of Sydney

8. Wang, Erpei. Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/18434

► Diseases caused by rust fungi represent critical constraints to global plant production. A characteristic feature of these pathogens is the striking pigments they produce in… (more)

▼ Diseases caused by rust fungi represent critical constraints to global plant production. A characteristic feature of these pathogens is the striking pigments they produce in one or more spore forms, which give them a rusty appearance. These pigments are thought to protect rust fungi against UV radiation and oxidative stress, and possibly act as virulence factors. The cytoplasmic carotenoid pigment composition and relative abundance of 14 rust species were studied, including Puccinia graminis f. sp. tritici (Pgt), P. striiformis f. sp. tritici, P. triticina, P. hordei, P. psidii, P. thaliae, P. oxalidis, Uromyces viciae-fabae, and Phragmidium rubi-idaei, along with colour mutants of several rust species with white, chocolate, pale yellow, dark brown, black, grey, yellow, yellow-orange, dark red and orange urediniospores. Four carotenoids had been found in rust fungi: phytoene, lycopene, -carotene and -carotene. The carotenoid composition did not vary much within rust species but, Among the 14 species investigated, the ratios of -/-carotene varied from 0.14 to 4.49, which might be used as an additional taxonomic character in rust fungi. Candidate genes for carotenoid biosynthesis in Pgt were identified, cloned and functionally complemented using specifically engineered strains of Escherichia coli. The carotenoid biosynthesis pathway in rust fungi was elucidated, with only two genes, CrtYB and CrtI, catalysing the reactions from GGPP to -carotene. Our understanding of carotenoid pigmentation evolution in rust fungi was improved by phylogenetic analysis. Both CrtYB and CrtI are closely related among rust fungi, other pathogenic fungi, and some aphids. A blackish-brown pigment was extracted from the wall of wild-type Pgt urediniospores. The spectrophotometric analysis results suggested that the spore wall pigment extracted from urediniospores of Pgt resembled the DOPA type melanin. Plausible structures of the fragments from this pigment were tentatively proposed.

Subjects/Keywords: Rusti fungi; Pigments; Carotenoid; Melanin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, E. (2018). Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/18434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Erpei. “Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution .” 2018. Thesis, University of Sydney. Accessed January 21, 2021. http://hdl.handle.net/2123/18434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Erpei. “Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution .” 2018. Web. 21 Jan 2021.

Vancouver:

Wang E. Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution . [Internet] [Thesis]. University of Sydney; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2123/18434.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang E. Pigments in Rust Fungi: Biosynthesis, Role in Plant-Pathogen Interactions, and Evolution . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/18434

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

9. Kha, Tuyen. Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder.

Degree: MPhil, 2010, University of Newcastle

URL: http://hdl.handle.net/1959.13/802339

►

Masters Research - Master of Philosophy (MPhil)

Gac fruit, Momordica cochinchinensis Spreng contains extraordinarily high levels of carotenoids, especially β-carotene and lycopene, and a comparatively… (more)

▼

Masters Research - Master of Philosophy (MPhil)

Gac fruit, Momordica cochinchinensis Spreng contains extraordinarily high levels of carotenoids, especially β-carotene and lycopene, and a comparatively high content of alpha-tocopherol (vitamin E) and of polyunsaturated fatty acids. The aim of this study was therefore to develop an understanding of suitable conditions for the processing of Gac fruit and the preparation of Gac fruit powder. The objectives of this study were to investigate the effect of 1) pre-treatments; blanching, ascorbic acid and bisulfite, and 2) drying techniques; air, vacuum, freeze and spray drying, on the physicochemical and antioxidant properties of powders produced from Gac arils. In addition, Gac arils (mixed with added maltodextrin) and untreated Gac skin and yellow pulp were air-dried and their properties were evaluated. The shelf life of a number of the Gac powder products was periodically evaluated during an extended storage period. The moisture sorption isotherms of various Gac powders were also constructed. Furthermore, the stability of three different types of Gac fruit powders was also tested when used in food and beverage products. Results showed that freeze drying of fresh Gac aril without any pre-treatment produced powders of high quality as determined by colour (hue angle of 33.93, total carotenoid content (TCC) of 7.24 mg/g and total antioxidant activity (TAA) of 0.39 mmole TE/g). However, pre-treatment of fresh Gac fruit aril with 1% (w/v) ascorbic acid or bisulfite solution before vacuum drying at 40 °C for 45 hours was just as effective as freeze drying in preserving colour (hue angle of 34.18 and 36.25, respectively), TCC (7.28 and 6.99 mg/g, respectively) and TAA of 0.36-0.40 mmole TE/g. Pre-treatment with 1% (w/v) ascorbic acid or bisulfite solution before air drying at 40 °C for 48 hours was also effective (TCC of 6.36 and 6.11 mg/g, respectively and TAA of 0.33 mmole TE/g) but not to the extent of vacuum or freeze drying. In respect of the spray drying process, taking into account the dilution effect of the added maltodextrin, the addition of 10% maltodextrin to the feed mixture and drying at 120 °C effectively preserved the physicochemical and antioxidant properties of the powder (hue angle of 66.85, TCC of 2.77 mg/g and TAA of 0.14 mmole TE/g). In addition, in a comparison of fresh and frozen arils, both were found to be equally useful for production of Gac powder in terms of preservation of colour (hue angle of 33.93 and 31.28, respectively), TCC (7.24 and 6.27 mg/g, respectively) and TAA (0.39 and 0.33 mmole TE/g, respectively). However, the dried aril was found to be very difficult to grind due to its stickiness. The addition of maltodextrin (0.5 or 1 g maltodextrin/g of total fruit solids) prior to drying solved this problem and also maintained the quality of the powder (hue angle of 28.04-30.55; TCC of 5.65-6.29 mg/g and TAA of 0.29-0.31 mmole TE/g, respectively). The storage study indicated that the degradation of TCC and TAA of freeze-dried and spray-dried…

Advisors/Committee Members: University of Newcastle. Faculty of Science and Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: Gac fruit powder; antioxidants; carotenoid; drying techniques

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kha, T. (2010). Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder. (Masters Thesis). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/802339

Chicago Manual of Style (16^th Edition):

Kha, Tuyen. “Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder.” 2010. Masters Thesis, University of Newcastle. Accessed January 21, 2021. http://hdl.handle.net/1959.13/802339.

MLA Handbook (7^th Edition):

Kha, Tuyen. “Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder.” 2010. Web. 21 Jan 2021.

Vancouver:

Kha T. Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder. [Internet] [Masters thesis]. University of Newcastle; 2010. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1959.13/802339.

Council of Science Editors:

Kha T. Effects of different drying processes on the physicochemical and antioxidant properties of gac fruit powder. [Masters Thesis]. University of Newcastle; 2010. Available from: http://hdl.handle.net/1959.13/802339

University of Manchester

10. Ogilvy, Victoria. THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION.

Degree: 2011, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:137588

► Amphibians are facing an extinction crisis, and in many cases it has become necessary to conserve species in captivity. Unfortunately, our understanding of the nutritional… (more)

▼ Amphibians are facing an extinction crisis, and in many cases it has become necessary to conserve species in captivity. Unfortunately, our understanding of the nutritional requirements of amphibians in captivity is currently limited. There have been anecdotal reports that captive bred amphibians have duller skin colouration than wild members of the same species. Many amphibians use carotenoid pigments in skin colouration. Carotenoids are yellow-red pigments synthesised by photosynthetic tissues, however vertebrates cannot synthesise them de novo and rely entirely on dietary sources. Reduced colour in captive bred amphibians could therefore result from limited carotenoid availability in the diet. Limited access to carotenoids could have further negative consequences on health and reproductive success since carotenoids are known to function in the immune, antioxidant and reproductive systems of other vertebrate taxa. The role of carotenoids in amphibian fitness is currently unknown, and the aim of this PhD was to assess the importance of carotenoids to anuran amphibians. Skin colour may be influenced by carotenoid availability, and it was therefore important to establish a suitable method for quantifying skin colour in amphibians. In Chapter 1 I assessed two methods for colour quantification, including digital photography and spectrometry. Neither method was significantly more accurate than the other, however I chose to use photography for my studies for reasons of practicality. In Chapters 2 and 3 I assessed the role of carotenoids in the reproductive systems of wild frogs. I found that carotenoid-based skin colouration was involved in the breeding behaviour of wild Agalychnis moreletii frogs. I found positive assortative mating by colour in that frogs were found mating with individuals with similar carotenoid-based colouration. This assortative mating by colour may be driven by mutual mate choice, or may have evolved to prevent mismatched pairing with a sympatric and phenotypically similar species (A. callidryas). I then analysed the carotenoid composition of eggs produced by wild A. moreletii and Phyllomedusa trinitatis, which are tree frogs with similar reproductive ecology but different egg deposition strategies: Agalychnis moreletii deposits green eggs on the surface of leaves while P. trinitatis wraps pale cream eggs in leaves. The concentration and diversity of carotenoids was significantly higher in A. moreletii than P. trinitatis and potential ecological explanations for this are discussed.In Chapter 4 I investigated carotenoid availability in the diet of captive amphibians by analysing the carotenoid composition of commonly used feeder-invertebrates. I assessed interspecific variation in carotenoid accumulation in three feeder-cricket species (Gryllus bimaculatus, Gryllodes sigillatus and Acheta domesticus), which were all fed one of three diets (wheat-bran, fish-food based diet, fresh fruit and vegetables). All three cricket species were a poor source of carotenoids unless they were gut-loaded on a… Advisors/Committee Members: Preziosi, Richard.

Subjects/Keywords: Amphibian; Nutrition; Carotenoid; ex situ conservation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ogilvy, V. (2011). THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:137588

Chicago Manual of Style (16^th Edition):

Ogilvy, Victoria. “THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION.” 2011. Doctoral Dissertation, University of Manchester. Accessed January 21, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:137588.

MLA Handbook (7^th Edition):

Ogilvy, Victoria. “THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION.” 2011. Web. 21 Jan 2021.

Vancouver:

Ogilvy V. THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Jan 21]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:137588.

Council of Science Editors:

Ogilvy V. THE INFLUENCE OF CAROTENOIDS ON FITNESS RELATED TRAITS IN ANURANS: IMPLICATIONS FOR EX SITU CONSERVATION. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:137588

University of Manchester

11. Ogilvy, Victoria. The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation.

Degree: PhD, 2011, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/the-influence-of-carotenoids-on-fitness-related-traits-in-anurans-implications-for-ex-situ-conservation(f1f58dbc-77ba-4a34-9378-886a215191a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606880

► Amphibians are facing an extinction crisis, and in many cases it has become necessary to conserve species in captivity. Unfortunately, our understanding of the nutritional… (more)

▼ Amphibians are facing an extinction crisis, and in many cases it has become necessary to conserve species in captivity. Unfortunately, our understanding of the nutritional requirements of amphibians in captivity is currently limited. There have been anecdotal reports that captive bred amphibians have duller skin colouration than wild members of the same species. Many amphibians use carotenoid pigments in skin colouration. Carotenoids are yellow-red pigments synthesised by photosynthetic tissues, however vertebrates cannot synthesise them de novo and rely entirely on dietary sources. Reduced colour in captive bred amphibians could therefore result from limited carotenoid availability in the diet. Limited access to carotenoids could have further negative consequences on health and reproductive success since carotenoids are known to function in the immune, antioxidant and reproductive systems of other vertebrate taxa. The role of carotenoids in amphibian fitness is currently unknown, and the aim of this PhD was to assess the importance of carotenoids to anuran amphibians. Skin colour may be influenced by carotenoid availability, and it was therefore important to establish a suitable method for quantifying skin colour in amphibians. In Chapter 1 I assessed two methods for colour quantification, including digital photography and spectrometry. Neither method was significantly more accurate than the other, however I chose to use photography for my studies for reasons of practicality. In Chapters 2 and 3 I assessed the role of carotenoids in the reproductive systems of wild frogs. I found that carotenoid-based skin colouration was involved in the breeding behaviour of wild Agalychnis moreletii frogs. I found positive assortative mating by colour in that frogs were found mating with individuals with similar carotenoid-based colouration. This assortative mating by colour may be driven by mutual mate choice, or may have evolved to prevent mismatched pairing with a sympatric and phenotypically similar species (A. callidryas). I then analysed the carotenoid composition of eggs produced by wild A. moreletii and Phyllomedusa trinitatis, which are tree frogs with similar reproductive ecology but different egg deposition strategies: Agalychnis moreletii deposits green eggs on the surface of leaves while P. trinitatis wraps pale cream eggs in leaves. The concentration and diversity of carotenoids was significantly higher in A. moreletii than P. trinitatis and potential ecological explanations for this are discussed. In Chapter 4 I investigated carotenoid availability in the diet of captive amphibians by analysing the carotenoid composition of commonly used feeder-invertebrates. I assessed interspecific variation in carotenoid accumulation in three feeder-cricket species (Gryllus bimaculatus, Gryllodes sigillatus and Acheta domesticus), which were all fed one of three diets (wheat-bran, fish-food based diet, fresh fruit and vegetables). All three cricket species were a poor source of carotenoids unless they were gut-loaded on a…

Subjects/Keywords: 597.8; Amphibian; Nutrition; Carotenoid; ex situ conservation

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❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ogilvy, V. (2011). The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/the-influence-of-carotenoids-on-fitness-related-traits-in-anurans-implications-for-ex-situ-conservation(f1f58dbc-77ba-4a34-9378-886a215191a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606880

Chicago Manual of Style (16^th Edition):

Ogilvy, Victoria. “The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation.” 2011. Doctoral Dissertation, University of Manchester. Accessed January 21, 2021. https://www.research.manchester.ac.uk/portal/en/theses/the-influence-of-carotenoids-on-fitness-related-traits-in-anurans-implications-for-ex-situ-conservation(f1f58dbc-77ba-4a34-9378-886a215191a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606880.

MLA Handbook (7^th Edition):

Ogilvy, Victoria. “The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation.” 2011. Web. 21 Jan 2021.

Vancouver:

Ogilvy V. The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Jan 21]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-influence-of-carotenoids-on-fitness-related-traits-in-anurans-implications-for-ex-situ-conservation(f1f58dbc-77ba-4a34-9378-886a215191a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606880.

Council of Science Editors:

Ogilvy V. The influence of carotenoids on fitness related traits in anurans : implications for ex situ conservation. [Doctoral Dissertation]. University of Manchester; 2011. Available from: https://www.research.manchester.ac.uk/portal/en/theses/the-influence-of-carotenoids-on-fitness-related-traits-in-anurans-implications-for-ex-situ-conservation(f1f58dbc-77ba-4a34-9378-886a215191a0).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606880

12. Bahari, Azlina. Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex.

Degree: PhD, 2019, University of Dundee

URL: https://discovery.dundee.ac.uk/en/studentTheses/73b6fffb-faf0-4fb9-94f1-aa9bbb330154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763006

► Hevea brasiliensis latex contains a large quantity of high molecular weight rubber and is thus the primary commercial source of natural rubber. Rubber and other… (more)

▼ Hevea brasiliensis latex contains a large quantity of high molecular weight rubber and is thus the primary commercial source of natural rubber. Rubber and other non-rubber isoprenoids in Hevea latex are synthesised from isopentenyl diphosphate (IPP) generated from the cytoplasmic mevalonate (MVA) pathway and the plastidic methyl erythritol phosphate pathway (MEP). This study utilised two rubber tree clones (RRIM600 and PB235) that show visibly contrasting levels of yellow carotenoids for the measurement of latex isoprenoids (carotenoids, rubber and isoprenoid intermediates) and transcript levels of the genes involved in isoprenoid biosynthesis. Metabolite extraction and analysis showed that four major carotenoids namely lutein, zeaxanthin, α-carotene and β-carotene were consistently present in both RRIM600 and PB235 latex. β-carotene was found to be the major carotenoid, at 1.2 μg/g in PB235 and 0.8 μg/g in RRIM600 fresh latex samples. However, the analytical method developed to measure isoprenoid intermediates needed to be further optimised to increase extraction efficiency. To enable accurate measurement of transcript levels of key genes involved in the isoprenoid biosynthetic pathway, a set of reference transcripts was constructed by merging short-reads (RNA-seq) and long-reads (Iso-seq and full-length cDNA sequences) data from Hevea brasiliensis. This produced a comprehensive set of 193,997 transcript sequences with good level of coverage of predicted transcripts and highly conserved core plant genes. Not only did the reference transcriptome update the annotation of rubber gene models, additional transcript variants were also discovered. Manual curation of gene models for key steps associated with rubber and carotenoids resulted in a repertoire of 115 genes, with 151 corresponding transcript variants. Subsequently, differential expression analysis on the basis of mapping RRIM600 and PB235 RNA-seq reads to the reference transcriptome revealed isoform-specific expression of genes for biosynthesis of carotenoids (PSY isoform 2), IPP (AACT2 and HMGR1) and rubber (REFSRPP gene members). In addition, the levels of these genes correlated positively with the carotenoid and rubber content measurements from the same latex of PB235 and RRIM600 used for metabolite extraction. Finally, the utility of the reference transcript catalogue was demonstrated by the characterisation of the REFSRPP gene family, which is involved in rubber elongation steps. REFSRPP gene family showed a local expansion which appear to be unique to Hevea. A pilot study has demonstrated there is considerable diversity of the genomic region containing the duplicated REFSRPP genes.

Subjects/Keywords: 678; latex; transcript; carotenoid; rubber biosynthesis

12 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bahari, A. (2019). Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex. (Doctoral Dissertation). University of Dundee. Retrieved from https://discovery.dundee.ac.uk/en/studentTheses/73b6fffb-faf0-4fb9-94f1-aa9bbb330154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763006

Chicago Manual of Style (16^th Edition):

Bahari, Azlina. “Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex.” 2019. Doctoral Dissertation, University of Dundee. Accessed January 21, 2021. https://discovery.dundee.ac.uk/en/studentTheses/73b6fffb-faf0-4fb9-94f1-aa9bbb330154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763006.

MLA Handbook (7^th Edition):

Bahari, Azlina. “Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex.” 2019. Web. 21 Jan 2021.

Vancouver:

Bahari A. Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex. [Internet] [Doctoral dissertation]. University of Dundee; 2019. [cited 2021 Jan 21]. Available from: https://discovery.dundee.ac.uk/en/studentTheses/73b6fffb-faf0-4fb9-94f1-aa9bbb330154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763006.

Council of Science Editors:

Bahari A. Approaches to understanding diversity in rubber and carotenoid synthesis in Hevea brasiliensis latex. [Doctoral Dissertation]. University of Dundee; 2019. Available from: https://discovery.dundee.ac.uk/en/studentTheses/73b6fffb-faf0-4fb9-94f1-aa9bbb330154 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763006

University of Illinois – Urbana-Champaign

13. Ford, Nikki A. Carotenoid metabolism in mice and prostate cancer risk.

Degree: PhD, 0191, 2011, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/18540

► Prostate cancer is the second most abundant cancer with a 32% mortality rate world-wide. Epidemiological studies suggest an inverse relationship between risk of prostate cancer… (more)

▼ Prostate cancer is the second most abundant cancer with a 32% mortality rate world-wide. Epidemiological studies suggest an inverse relationship between risk of prostate cancer and intake of tomato products or higher blood levels of lycopene. ??-carotene is centrally cleaved by carotene monoxygenase I (CMO-I) to form vitamin A and further metabolism results in formation of retinoic acid and other retinoids. The metabolism of ??-carotene has been extensively studied, but very little is known about the metabolism of other carotenoids. We and others have hypothesized that other acyclic carotenoids, like lycopene, are eccentrically cleaved by carotene monoxygenase II (CMO-II). Like retinoids, we propose that carotenoid metabolites produced by CMO-II enzymatic cleavage are bioactive at small concentrations in tissues. The primary aims of this proposal were to delineate tissue specific expression of CMO-I and CMO-II and evaluate resulting carotenoid bioaccumulation in CMO-I KO, CMO-II KO, and wild-type mice. Secondly, we investigated the effects of lycopene metabolties on human prostate cancer cells, in vitro. Thirdly, we investigated the effects of carotenoid metabolism in CMO-I KO, CMO-II KO, and WT mice on sex steroid hormone status. Lycopene preferentially accumulated in CMO-II KO mice while ??-carotene preferentially accumulated in CMO-I KO mice. Phytofluene and phytoene accumulation was not altered by genotype. Together these data suggest that lycopene is eccentrically metabolized by CMO-II and ??-carotene is centrally metabolized to retinal by CMO-I. Phytoene and phytofluene may not be substrates for either CMO-I or CMO-II cleavage or did not accumulate in high enough concentrations in the liver to induce cleavage by these enzymes. We also report that the mRNA expression of CMO-I and CMO-II were not altered by the carotenoid-containing diets used in iii our studies. Interestingly, serum and testes testosterone were reduced and related sex steroid metabolizing genes were altered in CMO-I KO mice. We hypothesize that due to induced expression of CMO-II in the testes of CMO-I KO mice that lycopenoids are at least in part responsible for these effects. Lastly, we demonstrate anti-proliferative effects of the lycopene-metabolite, apo-12???-lycopenal in androgen-dependent DU145 prostate cancer cells. Overall, our findings provide support for previous in vitro data to suggest that lycopene is metabolized by the CMO-II enzyme, in vivo. Furthermore, we have evidence to suggest that lycopene metabolites reduce proliferation of prostate cancer cells in vitro and may beneficially alter sex steroid status in mice. Advisors/Committee Members: Erdman, John W. (advisor), Nakamura, Manabu T. (Committee Chair), Erdman, John W. (committee member), Drackley, James K. (committee member), Jeffery, Elizabeth H. (committee member).

Subjects/Keywords: lycopene; carotenoid; prostate cancer; androgen; estrogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ford, N. A. (2011). Carotenoid metabolism in mice and prostate cancer risk. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/18540

Chicago Manual of Style (16^th Edition):

Ford, Nikki A. “Carotenoid metabolism in mice and prostate cancer risk.” 2011. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 21, 2021. http://hdl.handle.net/2142/18540.

MLA Handbook (7^th Edition):

Ford, Nikki A. “Carotenoid metabolism in mice and prostate cancer risk.” 2011. Web. 21 Jan 2021.

Vancouver:

Ford NA. Carotenoid metabolism in mice and prostate cancer risk. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2011. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2142/18540.

Council of Science Editors:

Ford NA. Carotenoid metabolism in mice and prostate cancer risk. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2011. Available from: http://hdl.handle.net/2142/18540

Université Paris-Sud – Paris XI

14. Jallet, Denis. Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie.

Degree: Docteur es, Biologie, 2013, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2013PA112270

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Un excès d’énergie lumineuse peut être délétère pour les organismesphotosynthétiques ; en effet, il en résulte la formation d’espèces réactives de l’oxygène ausein des centres… (more)

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Un excès d’énergie lumineuse peut être délétère pour les organismesphotosynthétiques ; en effet, il en résulte la formation d’espèces réactives de l’oxygène ausein des centres réactionnels. Les cyanobactéries ont adopté divers mécanismes dephotoprotection afin de contrer ce phénomène. L’un d’eux repose sur l’activité de l’OrangeCarotenoid Protein (OCP), protéine soluble qui attache un kéto-caroténoïde (hydroxyechinenone).Subissant de fortes intensités de lumière bleu-verte, l’OCP se convertit d’uneforme inactive/orange vers sa forme active/rouge. L’OCP ainsi photoactivée possède la facultéd’interagir avec les phycobilisomes - principales antennes collectrices de lumière - induisantla dissipation de l’énergie collectée par ces gigantesques complexes sous forme de chaleur. Lapression d’excitation au niveau des centres réactionnels ainsi que la fluorescence du systèmedécroissent alors.L’OCP photoactivée se fixe au coeur des phycobilisomes qui sont majoritairementconstitués de protéines chromophorylées de la famille des allophycocyanines (APC). J’aiconstruit différentes souches mutantes de Synechocystis PCC 6803 en modifiant ousupprimant les sous-unités mineures d’APC (ApcD, ApcF et ApcE). Ces sous-unités jouent lerôle essentiel d’émetteurs terminaux des phycobilisomes, véhiculant l’énergie qu’ellesreçoivent à la Chlorophylle a. J’ai aussi démontré que le mécanisme photoprotectif associé àl’OCP chez ces mutants restait inchangé, aussi bien in vivo que in vitro. Ces résultatssuggèrent qu’aucun émetteur terminal n’est nécessairement requis pour l’attachement del’OCP aux phycobilisomes et sous-entendent que l’OCP interagit probablement avec unesous-unité majeure d’APC.Divers phycobilisomes, contenant 2, 3 ou 5 cylindres d’APC dans leur coeur, ont étéisolés à partir de cyanobactéries variées. Les OCPs de Synechocytis et d’Arthrospira ont étépurifiées à partir de souches mutantes de Synechocystis. J’ai alors mené une étude in vitro desinteractions entre ces OCPs et les phycobilisomes. Le nombre de cylindres d’APC présents ausein des phycobilisomes n’affecte en rien la diminution de fluorescence. De plus, j’ai constatéque l’OCP de Synechocystis est spécifique pour ses propres phycobilisomes alors que l’OCPd’Arthrospira interagit avec tous les phycobilisomes employés ici. Des hypothèses, fondéessur les structures disponibles, ont été formulées pour élucider ces différences.Les domaines N- et C-terminaux de l’OCP d’Arthrospira ont été dissociés parprotéolyse. Le domaine N-terminal isolé conserve le caroténoïde attaché, ayant uneconformation similaire à celle observée lorsque l’OCP est photoactivée. Ce domaine Nterminalest aussi capable d’induire une importante diminution de la fluorescence desphycobilisomes. A l’inverse, le domaine C-terminal isolé est incolore et n’a aucun effet sur lafluorescence des phycobilisomes. Ces résultats suggèrent que seul le domaine N-terminal del’OCP est impliqué dans l’interaction avec les phycobilisomes. Le domaine C-terminal quantà lui module son activité.

Too much light can be lethal for…

Advisors/Committee Members: Kirilovsky, Diana (thesis director).

Subjects/Keywords: Cyanobactéries; Photoprotection; Orange Carotenoid Protein; Phycobilisomes; Cyanobacteria; Photoprotection; Orange Carotenoid Protein; Phycobilisomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jallet, D. (2013). Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2013PA112270

Chicago Manual of Style (16^th Edition):

Jallet, Denis. “Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie.” 2013. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 21, 2021. http://www.theses.fr/2013PA112270.

MLA Handbook (7^th Edition):

Jallet, Denis. “Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie.” 2013. Web. 21 Jan 2021.

Vancouver:

Jallet D. Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2013. [cited 2021 Jan 21]. Available from: http://www.theses.fr/2013PA112270.

Council of Science Editors:

Jallet D. Interactions between the Orange Carotenoid Protein and the phycobilisomes in cyanobacterial photoprotection : Interactions entre l’Orange Carotenoid Protein et les phycobilisomes dans un mécanisme de photoprotection chez les cyanobactérie. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2013. Available from: http://www.theses.fr/2013PA112270

Florida International University

15. Fang, Nan. Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus).

Degree: PhD, Chemistry, 2016, Florida International University

URL: https://digitalcommons.fiu.edu/etd/2600 ; 10.25148/etd.FIDC000724 ; FIDC000724

► This dissertation describes the purification and partial characterization of CBP from the epidermis of the monarch butterfly larvae (Danaus plexippus). A yellow protein-carotenoid complex… (more)

Subjects/Keywords: Carotenoid; carotenoid binding protein; lutein; fluorescence; surface plasmon resonance; Amino Acids, Peptides, and Proteins; Biochemistry; Carbohydrates; Equipment and Supplies

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fang, N. (2016). Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus). (Doctoral Dissertation). Florida International University. Retrieved from https://digitalcommons.fiu.edu/etd/2600 ; 10.25148/etd.FIDC000724 ; FIDC000724

Chicago Manual of Style (16^th Edition):

Fang, Nan. “Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus).” 2016. Doctoral Dissertation, Florida International University. Accessed January 21, 2021. https://digitalcommons.fiu.edu/etd/2600 ; 10.25148/etd.FIDC000724 ; FIDC000724.

MLA Handbook (7^th Edition):

Fang, Nan. “Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus).” 2016. Web. 21 Jan 2021.

Vancouver:

Fang N. Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus). [Internet] [Doctoral dissertation]. Florida International University; 2016. [cited 2021 Jan 21]. Available from: https://digitalcommons.fiu.edu/etd/2600 ; 10.25148/etd.FIDC000724 ; FIDC000724.

Council of Science Editors:

Fang N. Extraction, Purification and partial Characterization of a Carotenoid Binding Protein (CBP) from the Epidermis of the Monarch Butterfly Larvae (Danaus plexippus). [Doctoral Dissertation]. Florida International University; 2016. Available from: https://digitalcommons.fiu.edu/etd/2600 ; 10.25148/etd.FIDC000724 ; FIDC000724

University of Alberta

16. Klassen, Jonathan L. Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis.

Degree: PhD, Department of Biological Sciences, 2009, University of Alberta

URL: https://era.library.ualberta.ca/files/sb397946q

► Many diverse microbes have been detected in or isolated from glaciers, including novel taxa exhibiting previously unrecognized physiological properties with significant biotechnological potential. Of 29… (more)

▼ Many diverse microbes have been detected in or isolated from glaciers, including novel taxa exhibiting previously unrecognized physiological properties with significant biotechnological potential. Of 29 unique phylotypes isolated from Victoria Upper Glacier, Antarctica (VUG), 12 were related to the poorly studied bacterial genus Hymenobacter including several only distantly related to previously described taxa. Further study of these microorganisms revealed genotypic, phenotypic, morphological and chemotaxonomic divergence from named species and suggested that they likely represent novel Hymenobacter species. These studies also indicated, however, that the systematics of Hymenobacter and related microorganisms is more complex than previously realized, and may exhibit poorly defined species boundaries due to cosmopolitan dispersal, significant rates of horizontal gene transfer and reintroduction of archived genotypes, e.g., from glacial ice. These processes are reflected in the carotenoid composition of Hymenobacter and related organisms, which includes several novel methyl- and xylosyl-derivatives of 2'-hydroxyflexixanthin with distributions indicative of horizontal gene transfer or differential gain and/or loss of terminal biosynthetic pathway steps. These processes have been previously underappreciated in assessments of microbial carotenoid diversity and suggest the need for fine-scale phylogenetic study of carotenoid distribution in other microbial taxa. Further comparative genomics-based evaluation of microbial carotenoid biosynthesis indicated its wide phylogenetic distribution and diversification, controlled by several lineage-specific modes of evolution including horizontal transfer, de novo enzyme evolution followed by differential gene loss, co-evolution with biochemically associated structures and elevated mutation rates. The latter especially interacts with horizontal transfer depending on metabolic pathway topology, exemplified by the evolution of purple bacterial carotenoid biosynthesis. Exploration of VUG microbial diversity, therefore, not only revealed novel taxa and biotechnologically interesting compounds but also spurred broader evaluation of the mechanisms of metabolic pathway evolution applicable to many other taxa and biochemical pathways.

Subjects/Keywords: 2'-hydroxyflexixanthin; Hymenobacter; Evolution; Horizontal Gene Transfer; Glacier; Carotenoid; Species

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Klassen, J. L. (2009). Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/sb397946q

Chicago Manual of Style (16^th Edition):

Klassen, Jonathan L. “Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis.” 2009. Doctoral Dissertation, University of Alberta. Accessed January 21, 2021. https://era.library.ualberta.ca/files/sb397946q.

MLA Handbook (7^th Edition):

Klassen, Jonathan L. “Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis.” 2009. Web. 21 Jan 2021.

Vancouver:

Klassen JL. Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis. [Internet] [Doctoral dissertation]. University of Alberta; 2009. [cited 2021 Jan 21]. Available from: https://era.library.ualberta.ca/files/sb397946q.

Council of Science Editors:

Klassen JL. Carotenoid diversity in novel Hymenobacter strains isolated from Victoria Upper Glacier, Antarctica, and implications for the evolution of microbial carotenoid biosynthesis. [Doctoral Dissertation]. University of Alberta; 2009. Available from: https://era.library.ualberta.ca/files/sb397946q

Universidade Federal de Viçosa

17. Wilton Soares Cardoso. Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação.

Degree: 2007, Universidade Federal de Viçosa

URL: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1169

► min A deficiency is the leading cause of blindness in the world, reaching approximately 21% of all the mal nourished children, with the largest number… (more)

▼ min A deficiency is the leading cause of blindness in the world, reaching approximately 21% of all the mal nourished children, with the largest number of affected kids in parts of Asia and Africa. Biofortification of the maize with provitamin A carotenoids, through breeding, is an alternative to diminish this deficiency, mainly in poor countries. The maize grain is one of main staple food in poor areas reached by hipovitaminose A. Maize possess carotenoids with variability in content and profile, what makes breeding a possibility. Carotenoids are liposoluble compounds found in plants, microorganisms and some animals, and of the 600 existing types, about 50 carotenoids possess provitamin A activity. Of these substances, the β-carotene, is the main target for genetic improvement. The objective of this work was the characterization of 134 maize genotypes for carotenoids content and profile. All seeds came from the experimental fields and the Active Gene Bank of Embrapa Milho and Sorgo, at Sete Lagoas, MG, Brazil. The genotypes were commercial hybrids and varieties from national trials, inbred lines developed by the Embrapa biofortification breeding program and other accesses chosen due its yellow-orange endosperm color. The analyses were carried out in duplicates by spectrophotometric method to quantify the content of total carotenoids, carotenes, monohidroxylados and xanthophylls. The variations for the following traits: total carotenoids (9.46 to 42.84 μg/g), carotenes (0.88 to 4.93 μg/g), xanthophylls monohidroxylated (1.13 to 7.22 μg/g) and xanthophylls diidroxylated (5.55 to 34.11 μg/g). The average total carotenoid was 22.34 μg/g and the inbred line 541332 averaged 42.84 μg/g. Carotene average was of 2.66 g/g, being the largest concentration found for BRS 1001 (4.93 g/g); the materials analyzed averaged monohidroxylated and xanthophylls levels of 3.88 and 16.93 gμ/g, respectively, with prominence for two elite inbreds, 540755 with 7.22 μg/g of monohidroxylated and 541312-1 with 34.11 μg/g of xanthophylls. Comparing these results to ordinary averages it can be observed that the Embrapa germplasma has good variability and high potential for development of biofortified lines, mainly for total of carotenoids provitamin A. By multivariate analysis, the genotypes were grouped by the Tocher method in 18 groups, having as dissimilarity measure the average Euclidian distance. However, there are great genetic similarities among the entries. For selection of genotypes for genetic improvement, the materials were chosen for greater content of total carotenoids and carotenes and those of divergent groups shown in the Tocher grouping. In addition, for each genotype was established using the ratio monohidroxylated/carotenes and xanthophylls/monohidroxylated, two indices, to indicate tendencies to produce monohidroxilados and xanthophylls. Thus, four ideotypes were established taking in consideration the lower indices, higher contents of total carotenoids and also higher proportion… Advisors/Committee Members: João Carlos Cardoso Galvão, Múcio Silva Reis, Aluízio Borém de Oliveira, Maria Cristina Dias Paes, Flavio Dessaune Tadin.

Subjects/Keywords: Milho; Carotenóides; Biofortificação; Vitamina A; FITOTECNIA; Maize; Carotenoid; Biofortification; Vitamin A

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cardoso, W. S. (2007). Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação. (Thesis). Universidade Federal de Viçosa. Retrieved from http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1169

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cardoso, Wilton Soares. “Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação.” 2007. Thesis, Universidade Federal de Viçosa. Accessed January 21, 2021. http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1169.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cardoso, Wilton Soares. “Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação.” 2007. Web. 21 Jan 2021.

Vancouver:

Cardoso WS. Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação. [Internet] [Thesis]. Universidade Federal de Viçosa; 2007. [cited 2021 Jan 21]. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1169.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cardoso WS. Variabilidade de genótipos de milho quanto à composição de carotenóides nos grãos visando a biofortificação. [Thesis]. Universidade Federal de Viçosa; 2007. Available from: http://www.tede.ufv.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=1169

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Anonymous, Mahmooduzzafar. Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;.

Degree: Botany, 1991, Aligarh Muslim University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/53855

Abstract available newline newline

Bibliography p. i-xxxi

Advisors/Committee Members: Iqbal, Muhammad.

Subjects/Keywords: Pollution; Morphology; Cassia; Carotenoid; Chlorophyll

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APA (6^th Edition):

Anonymous, M. (1991). Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;. (Thesis). Aligarh Muslim University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/53855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anonymous, Mahmooduzzafar. “Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;.” 1991. Thesis, Aligarh Muslim University. Accessed January 21, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/53855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anonymous, Mahmooduzzafar. “Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;.” 1991. Web. 21 Jan 2021.

Vancouver:

Anonymous M. Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;. [Internet] [Thesis]. Aligarh Muslim University; 1991. [cited 2021 Jan 21]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53855.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anonymous M. Air pollution influence on morphology and growth of cassia sophera L and Sida rhombifolia L;. [Thesis]. Aligarh Muslim University; 1991. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/53855

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Kozlova, Tatiana. The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490).

Degree: Biosystems Engineering, 2019, University of Manitoba

URL: http://hdl.handle.net/1993/33964

► The production of biofuels and high-valuae biochemical commodities from microalgae may be considered “third generation biofuels”. Interest in the use of wastewaters for the industrial… (more)

▼ The production of biofuels and high-valuae biochemical commodities from microalgae may be considered “third generation biofuels”. Interest in the use of wastewaters for the industrial production of microalgae for biofuels and bioproducts has stimulated the studies on the influence of a variety of compounds in wastewaters on microalgae physiology. In this study the influence of four phytohormones (brassinolide, BL; 24-epi-brassinolide, EBL; 3-indole acetic acid, IAA, and abscisic acid, ABB) singularly and in some combinations, and two animal sex hormones (17β-estradiol, E2 and 17, 20β-dihydroxy-4-pregnen-3-one, 17,20β-P), on the physiology of Scenedesmus quadricauda was tested. The influence of 17β-estradiol on physiology of S. quadricauda and the Lemna minor grown in three different media (Bolt Basal Medium, natural Fishery Wastewater, and Reconstituted Fishery Wastewater) was also investigated. All the hormones tested had positive stimulatory effects on the growth and biosynthetic activity of S. quadricauda. EBL and IAA most induced pigments accumulation while the effect on fatty acids accumulation followed the order of: IAA ≥ ABA > EBL > BL. Both the quantities of fatty acids and their profiles depended on the hormone type and the specific concentrations tested. Synergistic increases in S. quadricauda biomass production, pigments accumulation, and neutral lipid accumulation were observed when EBL and IAA were assessed in combined-hormones trials. The stimulatory effect was 1.7-, 2.7-, and 3.3-fold greater for chlorophyll-a, total carotenoids, and fatty acids, respectively compared with single hormone treatments. Both animal steroids tested, E2 and 17,20β-P, demonstrated positive stimulatory effects on S. quadricauda cell growth parameters, pigments, and lipid accumulation. A comparison of E2 performance in three types of growth media (BBM, FWW and RFWW) revealed differences in the ability of E2 to induce growth and biosynthetic activities of both S. quadricauda and L. minor. The most effective E2 concentrations were also evidently different for the algae and the plant when they were grown in the same type of medium. Our results highlight the possibility that hormones could be used as a tool for algal biosynthesis manipulation in the biofuel, pharmaceutical and cosmetic industries. Advisors/Committee Members: Levin, David (Biosystems Engineering) (supervisor), Sri Ranjan, Ramanathan (Biosystems Engineering).

Subjects/Keywords: Phytohormones; Fish steroids; Microalgae; Chlorophyll-a; Carotenoid; Lipids

9 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kozlova, T. (2019). The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490). (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/33964

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kozlova, Tatiana. “The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490).” 2019. Thesis, University of Manitoba. Accessed January 21, 2021. http://hdl.handle.net/1993/33964.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kozlova, Tatiana. “The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490).” 2019. Web. 21 Jan 2021.

Vancouver:

Kozlova T. The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490). [Internet] [Thesis]. University of Manitoba; 2019. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1993/33964.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kozlova T. The influence of plant and animal hormones on growth and accumulation of pigments and fatty acids in the microalgae Scenedesmus quadricauda (CPCC-158) and the duckweed Lemna minor (CPCC-490). [Thesis]. University of Manitoba; 2019. Available from: http://hdl.handle.net/1993/33964

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

20. Read, Andrew. Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products.

Degree: MS, Department of Human Health and Nutritional Sciences, 2012, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3288

► Lutein is a lipid soluble plant pigment with recognized health benefits, although intake levels by the general population and bioavailability are generally low. These factors… (more)

Subjects/Keywords: lutein; carotenoid; in vitro; bioaccessibility; bioavailability; functional food; bakery

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APA (6^th Edition):

Read, A. (2012). Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3288

Chicago Manual of Style (16^th Edition):

Read, Andrew. “Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products.” 2012. Masters Thesis, University of Guelph. Accessed January 21, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3288.

MLA Handbook (7^th Edition):

Read, Andrew. “Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products.” 2012. Web. 21 Jan 2021.

Vancouver:

Read A. Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products. [Internet] [Masters thesis]. University of Guelph; 2012. [cited 2021 Jan 21]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3288.

Council of Science Editors:

Read A. Influence of Digestion Model, Product Type, and Enrichment Level on in vitro Bioavailability of Lutein from High Lutein Functional Bakery Products. [Masters Thesis]. University of Guelph; 2012. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3288

University of California – San Diego

21. Gaidarenko, Olga Dmitrievna. Carotenoid biosynthesis and productivity in diatoms.

Degree: Biology, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/6c13j7mg

► Due to their versatility and modest cultivation requirements, microalgae are a promising potential source of sustainable fuel, chemicals, and food. At present, microalgal production at… (more)

▼ Due to their versatility and modest cultivation requirements, microalgae are a promising potential source of sustainable fuel, chemicals, and food. At present, microalgal production at scale is not economically viable. A major hurdle to productivity is the inefficient use of light energy by dense microalgal cultures. Due to extensive photopigmentation, microalgae closest to the light source absorb more light than they can use, and wastefully dissipate the rest. As a result, light penetrance into the culture is steeply attenuated. Reducing light-harvesting or dissipation capacity of microalgal cells is a promising solution to uneven light distribution in mass cultures. Most efforts to do so have focused on chlorophytes, with some successes. Diatoms are a class of microalgae that is very promising in terms of productivity and has evolved light harvesting and photoprotective strategies that differ substantially from those utilized by chlorophytes. This dissertation explores the notion of improving diatom productivity through manipulating their light-harvesting and dissipation capabilities. Because microalgal performance in production conditions can differ substantially from what is observed in the laboratory, the responses of a wild-type production candidate diatom to simulated outdoor conditions are examined in Chapter 1. Substantial diel changes in hypothetical product yields were observed and discussed in terms of what variables need to be optimized to maximize productivity. Main light-harvesting and photoprotective carotenoid-derived photopigments were found to respond differently to chloroplast division and changes in irradiance, suggesting differential regulation. Chapter 2 examined carotenoid biosynthesis in diatoms, because diatom carotenoids play major light-harvesting and photoprotective roles. Targets for genetic manipulation were identified, transgenic lines with two distinct altered photopigmentation phenotypes were generated, and a model for how diatom carotenoid biosynthesis may be differentially regulated in response to chloroplast division and irradiance increase was developed. Chapter 3 focused on examining photosynthetic performance, growth, and productivity of two transgenic strains created in Chapter 2 and identified a strategy that may substantially improve diatom productivity. Overall, the dissertation substantially advances the understanding of diatom carotenoid biosynthesis, identifies strategies for improving light utilization efficiency in diatom cultures, and contributes to the understanding of practices to maximize the productivity of commercial microalgal cultivation.

Subjects/Keywords: Biology; Microbiology; Molecular biology; Carotenoid; Diatom; Fucoxanthin; Microalgae; Photopigment; Triacylglycerol

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APA (6^th Edition):

Gaidarenko, O. D. (2018). Carotenoid biosynthesis and productivity in diatoms. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/6c13j7mg

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gaidarenko, Olga Dmitrievna. “Carotenoid biosynthesis and productivity in diatoms.” 2018. Thesis, University of California – San Diego. Accessed January 21, 2021. http://www.escholarship.org/uc/item/6c13j7mg.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gaidarenko, Olga Dmitrievna. “Carotenoid biosynthesis and productivity in diatoms.” 2018. Web. 21 Jan 2021.

Vancouver:

Gaidarenko OD. Carotenoid biosynthesis and productivity in diatoms. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Jan 21]. Available from: http://www.escholarship.org/uc/item/6c13j7mg.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gaidarenko OD. Carotenoid biosynthesis and productivity in diatoms. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/6c13j7mg

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Texas

22. Hudelson, Karista. Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA.

Degree: 2011, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc103331/

► Copepods in high elevation lakes and ponds in Colorado are exposed to significant levels of ultraviolet radiation (UV), necessitating development of UV avoidance behavior and… (more)

Subjects/Keywords: Copepod; ultraviolet radiation; carotenoid; astaxanthin; zooplankton; high elevation lake

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APA (6^th Edition):

Hudelson, K. (2011). Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc103331/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hudelson, Karista. “Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA.” 2011. Thesis, University of North Texas. Accessed January 21, 2021. https://digital.library.unt.edu/ark:/67531/metadc103331/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hudelson, Karista. “Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA.” 2011. Web. 21 Jan 2021.

Vancouver:

Hudelson K. Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA. [Internet] [Thesis]. University of North Texas; 2011. [cited 2021 Jan 21]. Available from: https://digital.library.unt.edu/ark:/67531/metadc103331/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hudelson K. Ultraviolet Radiation Tolerance in High Elevation Copepods from the Rocky Mountains of Colorado, USA. [Thesis]. University of North Texas; 2011. Available from: https://digital.library.unt.edu/ark:/67531/metadc103331/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

23. Hoang, Van Chuyen. Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng).

Degree: PhD, 2018, University of Newcastle

URL: http://hdl.handle.net/1959.13/1390685

►

Research Doctorate - Doctor of Philosophy (PhD)

Gac fruit (Momordica cochinchinensis Spreng.) is a rich source of carotenoids for the manufacture of powder, oil and… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

Gac fruit (Momordica cochinchinensis Spreng.) is a rich source of carotenoids for the manufacture of powder, oil and capsules for food, cosmetic and pharmaceutical uses. Currently, only the aril of the Gac fruit is processed and the peel, like the other components, is discarded although it contains high level of carotenoids. Thus, if these carotenoids can be extracted effectively, the peel might become a potential source of these carotenoids and add value to what is otherwise a waste product. This research aimed at: 1) investigating the effects of different drying and treatment conditions for the preservation of carotenoids and antioxidant capacity in Gac peel ; 2) determining optimal conditions for recovering carotenoids and antioxidant capacity from the dried peel ; and 3) identifying optimal conditions for encapsulating the extracted carotenoids in order to increase the storage stability of the carotenoids. The results showed that the pretreatment of Gac peel with ascorbic acid combined with hot air drying at 70°C led to the highest retention of β-carotene, lycopene, lutein, total carotenoid and antioxidant capacity in the dried Gac peel. For the extraction of the dried peel, ethyl acetate was the most suitable solvent for the concurrent extraction of carotenoids and antioxidant capacity. The maximum carotenoid yield of 271 mg/100g DW was achieved by the conventional extraction carried out at 40.7°C for 150 minutes with a ratio of 80:1 of solvent volume to solid weight (mL/g). The optimisation of the ultrasound-assisted extraction of Gac peel determined that 50°C and 250 W were the optimal temperature and ultrasonic power for the extraction with the present sample size (0.5 gram of Gac peel with 40mL of solvent). The extraction at optimal conditions resulted in a significant higher antioxidant capacity yield (822 µM TE/100g DW), shorter extraction time (76 minutes) and a comparable carotenoid extraction yield (269 mg/100g DW) compared to the conventional extraction of Gac peel. The carotenoids recovered in oil from Gac peel were successfully encapsulated by a mixture of whey protein concentrate and gum arabic (7:3, w/w) as the wall material using a spray dryer. The encapsulated powder obtained from the emulsion containing 24.5% total solids with the ratio of 3:10 (w/w) of the carotenoid-rich oil to the wall material showed the highest retention of total carotenoid and antioxidant capacity. The optimal inlet temperature and feeding rate of the emulsion were 160°C and 180mL/h, respectively. A 6-month storage study on the stability of carotenoids observed a significant higher retention of total carotenoid in the encapsulated powder compared to that in the non-encapsulated oil containing carotenoids from Gac peel. In conclusion, carotenoids and antioxidant capacity from Gac peel can be recovered successfully from Gac peel. The encapsulated carotenoids from Gac peel could be preserved for long storage and may be used for the carotenoid fortification of foods,…

Advisors/Committee Members: University of Newcastle. Faculty of Science, School of Environmental and Life Sciences.

Subjects/Keywords: gac fruit; peel; carotenoid; extraction; encapsulation; thesis by publication

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APA (6^th Edition):

Hoang, V. C. (2018). Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng). (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1390685

Chicago Manual of Style (16^th Edition):

Hoang, Van Chuyen. “Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng).” 2018. Doctoral Dissertation, University of Newcastle. Accessed January 21, 2021. http://hdl.handle.net/1959.13/1390685.

MLA Handbook (7^th Edition):

Hoang, Van Chuyen. “Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng).” 2018. Web. 21 Jan 2021.

Vancouver:

Hoang VC. Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng). [Internet] [Doctoral dissertation]. University of Newcastle; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1959.13/1390685.

Council of Science Editors:

Hoang VC. Recovering and encapsulating carotenoids from the peel of gac fruit (Momordica cochinchinensis Spreng). [Doctoral Dissertation]. University of Newcastle; 2018. Available from: http://hdl.handle.net/1959.13/1390685

Australian National University

24. Hou, Xin. Hunting for carotenoid-derived retrograde signals that regulate plastid development .

Degree: 2018, Australian National University

URL: http://hdl.handle.net/1885/143928

► In plants, carotenoids are essential for photosynthesis and photoprotection. However, carotenoids are not the end-products of the pathway: apocarotenoids are produced by carotenoid cleavage dioxygenases… (more)

▼ In plants, carotenoids are essential for photosynthesis and photoprotection. However, carotenoids are not the end-products of the pathway: apocarotenoids are produced by carotenoid cleavage dioxygenases (CCDs) or non-enzymatic processes. Apocarotenoids are more soluble or volatile than carotenoids, but they are not simply breakdown products as there can be modifications post cleavage and functions include hormones, volatiles or signals. Evidence is emerging for a class of apocarotenoids herein referred to as Apocarotenoid Signals (ACSs) that have regulatory roles throughout plant development beyond those ascribed to ABA and strigolactone. In the present study, we provide evidence that ACS2, a cis-carotenoid-derived retrograde signal, regulates plastid development during both skotomorphogenesis and photomorphogenesis. cis-carotenoids produced early in the carotenoid pathway may serve as substrates for the production of novel ACSs that regulate nuclear gene expression, metabolic homeostasis and leaf development. When and where they accumulate and what physiological functions they may serve in higher plants remain unclear. cis-carotenoids are not easily detected in most plant tissues, except in the absence of carotenoid isomerase (CRTISO) activity when photoisomerisation rate-limits the isomerisation of tetra-cis to all-trans-lycopene. The accumulation of cis-carotenoids in Arabidopsis crtiso mutant (carotenoid and chloroplast regulation 2, ccr2) tissues was observed in plant tissues grown under extended darkness (i.e. shorter photoperiod) and coincided with a perturbation in chloroplast development that caused leaf yellowing. A forward genetic screen identified an epistatic interaction between the ζ-carotene isomerase (ziso) and ccr2 which could restore plastid development, and revealed that di-cis-ζ-carotene, tri-cis-neurosporene and tetra-cis-lycopene are likely substrates for the generation of an ACS, named ACS2. Transcriptomics analysis of ccr2 ziso mutant tissues revealed that photosynthesis associated nuclear gene expression (PhANG) was activated through the down-regulation of genes involved in repressing photomorphogenesis. We identified an alternative splice mutant of det1, a repressor of photomorphogenesis, which could restore PLB formation and cotyledon greening following de-etiolation in ccr2. Chemical inhibition of carotenoid cleavage dioxygenase activity provided evidence that ACS2 posttranscriptionally maintains protochlorophyllide oxidoreductase (POR) protein levels acting downstream of DET1 to control PLB formation and plastid development. Phytoene synthase (PSY) is a major rate-controlling enzyme that catalyses the initial step of carotenoid biosynthesis and is hence under multi-level regulation. Alteration of PSY gene expression, protein levels or enzyme activity can…

Subjects/Keywords: Apocarotenoid Signal; cis-Carotenoid; Plastid Development; Prolamellar Body; Photomorphogenesis

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APA (6^th Edition):

Hou, X. (2018). Hunting for carotenoid-derived retrograde signals that regulate plastid development . (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/143928

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hou, Xin. “Hunting for carotenoid-derived retrograde signals that regulate plastid development .” 2018. Thesis, Australian National University. Accessed January 21, 2021. http://hdl.handle.net/1885/143928.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hou, Xin. “Hunting for carotenoid-derived retrograde signals that regulate plastid development .” 2018. Web. 21 Jan 2021.

Vancouver:

Hou X. Hunting for carotenoid-derived retrograde signals that regulate plastid development . [Internet] [Thesis]. Australian National University; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1885/143928.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hou X. Hunting for carotenoid-derived retrograde signals that regulate plastid development . [Thesis]. Australian National University; 2018. Available from: http://hdl.handle.net/1885/143928

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

25. Challa, Sneha. Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum.

Degree: 2015, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/2970

► Carotenoid cleavage dioxygenases (CCDs) are responsible for the cleavage of carotenoids into smaller compounds, including apocarotenoids. The volatile apocarotenoids produced have demonstrated a repellent and… (more)

Subjects/Keywords: beta-ionone; beta-caryophyllene; carotenoid cleavage dioxygenases; apocarotenoids; Biology

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APA (6^th Edition):

Challa, S. (2015). Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/2970

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Challa, Sneha. “Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum.” 2015. Thesis, University of Western Ontario. Accessed January 21, 2021. https://ir.lib.uwo.ca/etd/2970.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Challa, Sneha. “Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum.” 2015. Web. 21 Jan 2021.

Vancouver:

Challa S. Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum. [Internet] [Thesis]. University of Western Ontario; 2015. [cited 2021 Jan 21]. Available from: https://ir.lib.uwo.ca/etd/2970.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Challa S. Effect of carotenoid volatiles on oviposition and feeding choice of T.ni and T.vaporariorum. [Thesis]. University of Western Ontario; 2015. Available from: https://ir.lib.uwo.ca/etd/2970

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

26. Johnson, Ashley. The maintenance of variation in Cyprinodontiformes.

Degree: PhD, 0314, 2013, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/42447

► Maintenance of genetic variation in the face of strong natural selection is a long-standing problem in evolutionary biology. Two extreme examples of this are the… (more)

▼ Maintenance of genetic variation in the face of strong natural selection is a long-standing problem in evolutionary biology. Two extreme examples of this are the guppy (Poecilia reticulata) and the bluefin killifish (Lucania goodei), two freshwater species in the order Cyprinodontiformes with extensive within-population color pattern variation found among males. I use these two species as case studies to examine the evolution and maintenance of color pattern variation. First, I examine a potential reason behind the evolution of a female mating preference for males with novel color patterns in guppies. This preference has been implicated as a factor in maintaining the genetically-determined color pattern polymorphisms found in male guppies, and inbreeding avoidance has been proposed as a mechanism to explain its evolution. Inbreeding avoidance is advantageous when populations exhibit inbreeding depression and the opportunity for mating between relatives exists. To determine whether these conditions are met in a natural guppy population, I assessed mating and reproductive patterns using parentage analysis. Females produced more offspring with less-related males than with more-related ones. In addition, females were more likely to have mated with less-related males, but this trend was only marginally significant. Male heterozygosity was positively correlated with mating success and with the number of offspring sired, consistent with strong inbreeding depression for adult male fitness. These results show that strong inbreeding depression occurs in guppies, and individuals tend to avoid mating with relatives. Thus, the preference for novel male phenotypes may have evolved due to the advantage inherent to avoiding inbreeding. In my remaining chapters, I focus the bluefin killifish (Lucania goodei). These fish exhibit extensive color variation in their fins, but the function of this variation has not yet been determined. I collected males from multiple populations across Florida and used absorption spectroscopy to identify the pigments responsible for the fin coloration. I determined that orange coloration in the caudal fin was caused by a carotenoid pigment. Color in the anal fin was either pterin based (yellow and red) or structural (blue) with a melanic fin border. Using a behavioral assay designed to measure dominance, I sought to determine the informational content of each pigment. Black melanic markings on the anal fin were strongly related to dominance. Aggression was greater between males of similar sized melanic stripes, indicating that they functioned as badges of status in territorial interactions with other males. In keeping with their dietary origin, caudal carotenoid levels positively correlated with condition but did not influence dominance interactions. However, the highly labile ornament predicted parasite infection and spawning success, suggesting a role in intersexual selection, with caudal carotenoid as a signal of health to potential mates. Similarly, pterin pigmentation in the… Advisors/Committee Members: Fuller, Rebecca C. (advisor), Fuller, Rebecca C. (Committee Chair), Cáceres, Carla E. (committee member), Robinson, Gene E. (committee member), Hughes, Kimberly A. (committee member).

Subjects/Keywords: color variation; Lucania goodei; Poecilia reticulata; carotenoid; pterin; Melanin; opsin

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APA (6^th Edition):

Johnson, A. (2013). The maintenance of variation in Cyprinodontiformes. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/42447

Chicago Manual of Style (16^th Edition):

Johnson, Ashley. “The maintenance of variation in Cyprinodontiformes.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 21, 2021. http://hdl.handle.net/2142/42447.

MLA Handbook (7^th Edition):

Johnson, Ashley. “The maintenance of variation in Cyprinodontiformes.” 2013. Web. 21 Jan 2021.

Vancouver:

Johnson A. The maintenance of variation in Cyprinodontiformes. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2142/42447.

Council of Science Editors:

Johnson A. The maintenance of variation in Cyprinodontiformes. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/42447

27. Robinett, Sheldon J. (Sheldon Jay). Studies on the carotenoid pigments of Corynebacterium poinsettiae.

Degree: 1980, North Texas State University

URL: https://digital.library.unt.edu/ark:/67531/metadc798229/

Our research has shown the carotenoid pigment to be found in the cell membrane of C. poinsettiae. These results suggest that the pigment is bound to protein and that protein tertiary structure is important in the binding.

Subjects/Keywords: carotenoids; Corynebacterium poinsettiae; carotenoid pigments

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APA (6^th Edition):

Robinett, S. J. (. J. (1980). Studies on the carotenoid pigments of Corynebacterium poinsettiae. (Thesis). North Texas State University. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc798229/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Robinett, Sheldon J (Sheldon Jay). “Studies on the carotenoid pigments of Corynebacterium poinsettiae.” 1980. Thesis, North Texas State University. Accessed January 21, 2021. https://digital.library.unt.edu/ark:/67531/metadc798229/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Robinett, Sheldon J (Sheldon Jay). “Studies on the carotenoid pigments of Corynebacterium poinsettiae.” 1980. Web. 21 Jan 2021.

Vancouver:

Robinett SJ(J. Studies on the carotenoid pigments of Corynebacterium poinsettiae. [Internet] [Thesis]. North Texas State University; 1980. [cited 2021 Jan 21]. Available from: https://digital.library.unt.edu/ark:/67531/metadc798229/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Robinett SJ(J. Studies on the carotenoid pigments of Corynebacterium poinsettiae. [Thesis]. North Texas State University; 1980. Available from: https://digital.library.unt.edu/ark:/67531/metadc798229/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Kansas State University

28. Shen, Yanting. Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A.

Degree: MS, Food Science Institute - Food, Nutrition, Dietetics and Health, 2016, Kansas State University

URL: http://hdl.handle.net/2097/34473

► Sorghum is a staple crop consumed in certain regions of Africa and Asia, where vitamin A deficiency is prevalent. However, the correlation of sorghum intake… (more)

Subjects/Keywords: Sorghum; Vitamin A deficiency; Pericarp pigment; Pro-vitamin A; Carotenoid

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APA (6^th Edition):

Shen, Y. (2016). Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A. (Masters Thesis). Kansas State University. Retrieved from http://hdl.handle.net/2097/34473

Chicago Manual of Style (16^th Edition):

Shen, Yanting. “Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A.” 2016. Masters Thesis, Kansas State University. Accessed January 21, 2021. http://hdl.handle.net/2097/34473.

MLA Handbook (7^th Edition):

Shen, Yanting. “Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A.” 2016. Web. 21 Jan 2021.

Vancouver:

Shen Y. Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A. [Internet] [Masters thesis]. Kansas State University; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2097/34473.

Council of Science Editors:

Shen Y. Sorghum pericarp pigments are associated with the contents of carotenoids and provitamin A. [Masters Thesis]. Kansas State University; 2016. Available from: http://hdl.handle.net/2097/34473

University of Kentucky

29. Shenoy, Kausalya. THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES.

Degree: 2012, University of Kentucky

URL: https://uknowledge.uky.edu/biology_etds/26

► Male mating signals convey important mate-quality information to females and are regulated by androgens. Endocrine disrupting compounds (EDCs) are chemicals that interfere with proper hormonal… (more)

▼ Male mating signals convey important mate-quality information to females and are regulated by androgens. Endocrine disrupting compounds (EDCs) are chemicals that interfere with proper hormonal functioning in exposed animals, causing altered hormone levels and resulting in changed reproductive characteristics, including mating signals. Altered signals can have ecological implications by influencing population and community dynamics and evolutionary implications via trans-generational reduction in signal reliability leading to reduced preference and eventual loss of the signal trait. I examined the effects of exposure to environmentally relevant concentrations of atrazine, a widely used herbicide and EDC, on mating signals and behaviors in male guppies, a sexually dimorphic freshwater fish. Guppies were exposed either during adulthood or embryonic development. Prolonged atrazine exposure during adulthood reduced the size of the carotenoid-based ornament, the number of courtship displays performed, and aggression towards competing males. Embryonic exposure did not affect survival to adulthood and the time to develop male-specific morphologies. But there was a trend for smaller genitalia, and the ornament size was significantly increased. Possible increases in immunocompetence as a result of slight estrogenecity may have allowed for greater carotenoid allocation to the ornament. Embryonic exposure also resulted in reduced courtship behavior, forced copulatory attempts and aggression towards competitors; female guppies found these males less attractive. The low dose had the strongest effects with embryonic exposure, indicating the importance of low-dose exposures. These studies highlight the effects of low and environmentally relevant doses of atrazine on mating signals and behaviors in exposed wildlife. A mathematical model was used to understand the evolutionary effects of EDCs on the optimal allocation of carotenoids between ornament and immunocompetence. Animals obtain carotenoids through their diet, and allocate some of this to enhance immune function and the rest to ornaments for mate attraction. The model replicates the disruption of carotenoid-based ornaments as a result of EDC-exposure, and predicts that signal reliability will be reduced. The model simulates an evolutionary shift in the optimal allocation if exposure spanned multiple generations, but signal reliability is not restored. Including additional selective forces like predation further suppresses signal reliability.

Subjects/Keywords: atrazine; carotenoid-based ornament; mating behaviors; Poecilia reticulata; sexual signaling; Biology

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APA (6^th Edition):

Shenoy, K. (2012). THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/biology_etds/26

Chicago Manual of Style (16^th Edition):

Shenoy, Kausalya. “THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES.” 2012. Doctoral Dissertation, University of Kentucky. Accessed January 21, 2021. https://uknowledge.uky.edu/biology_etds/26.

MLA Handbook (7^th Edition):

Shenoy, Kausalya. “THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES.” 2012. Web. 21 Jan 2021.

Vancouver:

Shenoy K. THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES. [Internet] [Doctoral dissertation]. University of Kentucky; 2012. [cited 2021 Jan 21]. Available from: https://uknowledge.uky.edu/biology_etds/26.

Council of Science Editors:

Shenoy K. THE ECOLOGICAL AND EVOLUTIONARY EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON SEXUALLY SELECTED TRAITS IN MALE GUPPIES. [Doctoral Dissertation]. University of Kentucky; 2012. Available from: https://uknowledge.uky.edu/biology_etds/26

University of Georgia

30. Pierce, Emily Coleen. Genetic engineering of ketocarotenoid production in soybean.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/28579

► Carotenoids are a group of natural pigments that can bioaccummulate and provide color to many animal products. The pink or red ketocarotenoids, canthaxanthin and astaxanthin,… (more)

Subjects/Keywords: metabolic engineering; carotenoid; soybean; transgenic plants; astaxanthin; canthaxanthin; E. coli

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APA (6^th Edition):

Pierce, E. C. (2014). Genetic engineering of ketocarotenoid production in soybean. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/28579

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pierce, Emily Coleen. “Genetic engineering of ketocarotenoid production in soybean.” 2014. Thesis, University of Georgia. Accessed January 21, 2021. http://hdl.handle.net/10724/28579.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pierce, Emily Coleen. “Genetic engineering of ketocarotenoid production in soybean.” 2014. Web. 21 Jan 2021.

Vancouver:

Pierce EC. Genetic engineering of ketocarotenoid production in soybean. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10724/28579.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pierce EC. Genetic engineering of ketocarotenoid production in soybean. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/28579

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations