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Technical University of Lisbon
1.
Pereira, Marta Ornelas Dantas.
Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos.
Degree: 2015, Technical University of Lisbon
URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10380 
► Dissertação de Mestrado Integrado em Medicina Veterinária
O cancro é das principais causas de morte nos animais domésticos. Além disso, muitas formas de neoplasia assemelham-se…
(more)
▼ Dissertação de Mestrado Integrado em Medicina Veterinária
O cancro é das principais causas de morte nos animais domésticos. Além disso, muitas formas de neoplasia assemelham-se às suas homólogas nos humanos, em termos de comportamento biológico, expressão génica e fatores de risco.
À semelhança do cancro mamário na Mulher, os tumores mamários malignos caninos representam um grupo heterogéneo em termos morfológicos e biológicos. Adicionalmente, a grande importância dos tumores mamários caninos na medicina canina prende-se com o facto destes representarem a neoplasia mais comum nas fêmeas desta espécie.
É sabido que na espécie humana, aproximadamente 15–30% do total de cancros mamários sobre-expressam o recetor HER-2, conferindo-lhe uma maior agressividade e pior sobrevivência. A clivagem proteolítica do HER-2 (HER-2 shedding) gera moléculas solúveis formadas apenas pelo domínio extracelular (DEC) do recetor e cuja concentração pode ser quantificada no soro. Recentemente, os níveis séricos do DEC (sHER-2) têm sido propostos como um biomarcador para a determinação do status do HER-2 nos tumores mamários.
Na cadela, alguns estudos demonstraram que a sobre-expressão do HER-2 ocorre em 9,8–48% dos tumores mamários malignos. No entanto, as possíveis analogias em termos prognósticos e preditivos entre os padrões da sobre-expressão proteica na espécie canina e humana é um tema que só recentemente começou a ser estudado e que para o qual existem resultados controversos.
O objetivo deste estudo foi avaliar os níveis séricos de HER-2 (com base na quantificação do DEC) em cadelas com carcinoma mamário e estudar a associação destes níveis com diferentes variáveis clínico-patológicas, no sentido de investigar o potencial deste recetor como alvo terapêutico.
A expressão do HER-2 foi avaliada em vinte e cinco amostras de soro, através da técnica de Dot Blot. Quinze (60%) animais com tumores mamários malignos apresentaram níveis aumentados de sHER-2, mostrando associação positiva com o tamanho tumoral. Os resultados obtidos sugerem que, na espécie canina, níveis aumentados de sHER-2 se associam a algumas características tumorais mais desfavoráveis, como reportado no cancro mamário na Mulher e, que a possibilidade do uso de anticorpos anti-HER-2 em cadelas com tumores mamários que mostrem sobre-expressão de HER-2 deverá ser avaliada.
ABSTRACT - Cancer is a major cause of death in domestic animals. Furthermore, many types of pet neoplasm resemble human counterparts in biological behavior, gene expression and risk factors.
Similarly to breast cancer in woman, canine malignant mammary tumors represent a heterogeneous group in terms of morphology and biology. Additionally, the great importance of canine mammary tumors in canine medicine it´s due to the fact that these represent the most common neoplasm in females of this species.
It is known that in humans, approximately 15–30% of breast cancers overexpress the HER-2 receptor, which confers greater aggressiveness and a worse survival. HER-2 proteolytic cleavage (HER-2 shedding)…
Advisors/Committee Members: Ferreira, Fernando António da Costa, Soares, Maria João da Costa.
Subjects/Keywords: Tumores mamários caninos; HER-2; Sobre-expressão; sHER-2; Dot Blot; Canine mammary tumors; Overexpression
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APA (6th Edition):
Pereira, M. O. D. (2015). Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10380
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pereira, Marta Ornelas Dantas. “Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos.” 2015. Thesis, Technical University of Lisbon. Accessed March 04, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10380.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pereira, Marta Ornelas Dantas. “Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos.” 2015. Web. 04 Mar 2021.
Vancouver:
Pereira MOD. Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos. [Internet] [Thesis]. Technical University of Lisbon; 2015. [cited 2021 Mar 04].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10380.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pereira MOD. Avaliação dos níveis serológicos de HER-2 : relevância clínica em cadelas com tumores mamários malignos. [Thesis]. Technical University of Lisbon; 2015. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/10380
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Georgia
2.
Johnson, Melanie E.
Survivin expression in canine and feline cancer.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/22411
► Survivin is an inhibitor of apoptosis protein that is expressed during embryonic development and several human malignancies but is absent in most adult tissues. Its…
(more)
▼ Survivin is an inhibitor of apoptosis protein that is expressed during embryonic development and several human malignancies but is absent in most adult tissues. Its expression is often associated with a more malignant phenotype and decreased
response to chemotherapeutics. Analysis of the expression profile of transcripts in various cancers identified survivin as one of the top four transcripts upregulated in malignancy. Four human survivin isoforms have been identified that differ in their
ability to inhibit apoptosis. Thus, these isoforms may represent a regulatory balance between apoptosis and inhibition of apoptosis. Intense research is currently under way in human medicine to investigate the role of survivin in cancer. However, nothing
is known in regards to survivin expression in veterinary species. In order to examine survivin expression in canine and feline tissues, immunohistochemistry (IHC) and RT-PCR were performed on dog and cat embryos, normal adult tissues, inflammatory and
hyperplastic tissues, and a variety of neoplastic tissues. Survivin was expressed in fetal tissues, some hyperplastic, inflammatory, and neoplastic tissues, but was absent in most normal, adult tissues. To determine its significance as a prognostic
parameter in canine cancer, survivin expression was determined in neoplastic lymph nodes from dogs undergoing chemotherapy for lymphosarcoma. Survivin expression was shown to be a negative predictor of survival in canine lymphosarcoma. Canine survivin
isoforms have yet to be identified. To explore the possibility of differential expression of canine survivin isoforms, survivin subcellular localization, and proliferative and apoptotic markers were compared between canine mammary adenomas and carcinomas
that were all positive for survivin by IHC. Differences in nuclear survivin expression, Ki-67, and caspase-3 between the tumor types were not observed, thus potential expression of different canine survivin isoforms was not apparent from our data. In
conclusion, results of this work suggest that survivin expression in veterinary species needs to be further investigated, as this protein may prove to be useful in the diagnosis and prognosis of animal cancers. Furthermore, survivin could potentially
become a therapeutic target in veterinary oncology in the future.
Subjects/Keywords: apoptosis inhibitor; cancer; canine lymphosarcoma; canine mammary tumors; caspase-3; cell division; Ki-67; prognostic indicator; review; survivin
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APA (6th Edition):
Johnson, M. E. (2014). Survivin expression in canine and feline cancer. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22411
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Johnson, Melanie E. “Survivin expression in canine and feline cancer.” 2014. Thesis, University of Georgia. Accessed March 04, 2021.
http://hdl.handle.net/10724/22411.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Johnson, Melanie E. “Survivin expression in canine and feline cancer.” 2014. Web. 04 Mar 2021.
Vancouver:
Johnson ME. Survivin expression in canine and feline cancer. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/10724/22411.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Johnson ME. Survivin expression in canine and feline cancer. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22411
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Swedish University of Agricultural Sciences
3.
Wensman, Helena.
Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas.
Degree: 2009, Swedish University of Agricultural Sciences
URL: http://pub.epsilon.slu.se/1977/
► Mammary tumors are the most common type of tumors affecting female dogs. There is a remarkable heterogeneity among mammary tumors and the underlying cause is…
(more)
▼ Mammary tumors are the most common type of tumors affecting female dogs. There is a remarkable heterogeneity among mammary tumors and the underlying cause is poorly understood. The aims of this thesis were, firstly, to investigate whether tumor cells show plasticity and thereby get insight into how heterogeneity arises and, secondly, to identify embryonic developmental genes expressed in the tumors. A comparison between sarcomas and carcinomas in these respects was of central importance for this investigation. Primary tumors, an osteosarcoma, a spindle-cell tumor, and a simple carcinoma were cloned and resulting clones were subcutaneously injected in mice, to form experimental tumors. By following the expression of lineage markers in the primary tumors, the clones in vitro, and in the experimental tumors, it was found that the sarcoma clones showed distinct signs of plasticity, and that the carcinoma clones transformed in line with an epithelial to mesenchymal transition. The expression of genes coding for the developmentally important bone morphogenetic proteins was studied in the clones from the tumors described above. Further, three groups of tumors, i.e., osteosarcomas, fibrosarcomas and simple carcinomas were studied using gene expression profiling technology. Thereby, extensive expression of homeobox genes, a group of important developmental genes, was identified. Sarcomas displayed the most prominent expression of developmental genes, both for the number of genes expressed and the levels of expression for the respective genes. Interestingly, many homeobox genes expressed in the sarcomas have been linked to craniofacial development, a process dependent on neural crest contribution. In line with this, cell markers for neural crest derived tissues were also expressed in the sarcomas, including tooth, nerve and glial markers. The results show that sarcomas are more plastic and have a more diverse gene expression than carcinomas. Heterogeneity seen in sarcomas of the mammary gland may be related to the expression of homeobox genes.
Subjects/Keywords: dogs; neoplasms; proteins; homeboxes; rheological properties; gene expression; Canine mammary tumors; plasticity; bone morphogenetic proteins; homeobox transcription factors; craniofacial; neural crest.
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APA (6th Edition):
Wensman, H. (2009). Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1977/
Chicago Manual of Style (16th Edition):
Wensman, Helena. “Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas.” 2009. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed March 04, 2021.
http://pub.epsilon.slu.se/1977/.
MLA Handbook (7th Edition):
Wensman, Helena. “Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas.” 2009. Web. 04 Mar 2021.
Vancouver:
Wensman H. Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2009. [cited 2021 Mar 04].
Available from: http://pub.epsilon.slu.se/1977/.
Council of Science Editors:
Wensman H. Differences in plasticity and expression of developmental genes in canine mammary Carcinomas and Sarcomas. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2009. Available from: http://pub.epsilon.slu.se/1977/
Université de Montréal
4.
Sonzogni-Desautels, Karine.
Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo.
Degree: 2009, Université de Montréal
URL: http://hdl.handle.net/1866/7174
Subjects/Keywords: Tumeurs mammaires canines; Canine mammary tumors; COX-2; COX-2; Piroxicam; Piroxicam; Deracoxib; Deracoxib; AINS; NSAID; Xénogreffes mammaires; Mammary xenografts; Coussinet graisseux mammaire; Mammary fat pad; Cancer mammaire; Mammary cancer; Chiens; Dogs; Prostaglandines; Prostaglandins
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APA ·
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APA (6th Edition):
Sonzogni-Desautels, K. (2009). Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo. (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/7174
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sonzogni-Desautels, Karine. “Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo.” 2009. Thesis, Université de Montréal. Accessed March 04, 2021.
http://hdl.handle.net/1866/7174.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sonzogni-Desautels, Karine. “Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo.” 2009. Web. 04 Mar 2021.
Vancouver:
Sonzogni-Desautels K. Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo. [Internet] [Thesis]. Université de Montréal; 2009. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/1866/7174.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sonzogni-Desautels K. Évaluation de l'efficacité d'inhibiteurs de la cyclooxygénase dans le traitement de tumeurs mammaires canines in vivo. [Thesis]. Université de Montréal; 2009. Available from: http://hdl.handle.net/1866/7174
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Erdélyi, Ildikó.
Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.
Degree: 2006, University Utrecht
URL: https://dspace.library.uu.nl/handle/1874/9192
;
URN:NBN:NL:UI:10-1874-9192
;
1874/9192
;
urn:isbn:9039341931
;
URN:NBN:NL:UI:10-1874-9192
;
https://dspace.library.uu.nl/handle/1874/9192
► The extracellular matrix (ECM) research has become fundamental to understand cancer. This thesis focuses on the exploration of ECM composition and organization in canine mammary…
(more)
▼ The extracellular matrix (ECM) research has become fundamental to understand cancer. This thesis focuses on the exploration of ECM composition and organization in canine mammary tumors, with a special interest in the large chondroitin-sulfate proteoglycan (PG), versican.
Chapter 1 gives an overview of the recent concepts in matrix biology, particularly in tumors, and introduces versican and other ECM molecules investigated in the following chapters.
In Chapter 2 we show that versican protein expression is increased in tumors. Versican accumulates at three main locations in tumors: (1) in periductal tissues; (2) in peripheral invasive areas; (3) and the G1-domain of versican in myoepithelial-like spindle cell proliferations and myxoid tissues of complex and mixed tumors.
In Chapter 3 we show up-regulated mRNA expression of versican, collagen type II and aggrecan in tumors, especially in complex and mixed tumors. Expression of cartilage biomarkers was noted not only in the cartilagenous tissues of mixed tumors, but in myoepitheliomas and in myoepithelial-like cell proliferations and myxoid tissues of complex and mixed tumors. Therefore, the latter tissues appear to be the precursor tissues of the ectopic cartilage in mixed tumors. In addition, co-localization of hyaluronan (HA) and link protein (LP) with versican surrounding invading tumor cells was demonstrated. The three molecules may form complexes in canine mammary tumors and provide a viscoelastic microenvironment that promotes tumor cell proliferation and migration.
Chapter 4 shows that versican is present in multiple forms, including splice variants and catabolic products. V1, V0 and V3 versican mRNA and protein have been identified, with a prevalence of V1/V0 variants in canine mammary tumors and cell lines. The tumor cell lines expressed increased mRNA and protein of V0 and V1 versican compared to the normal mammary fibroblasts and the myoepithelial-like tumor cells expressed the highest levels of all mRNA splice variants. The high expression of the hydrophilic V0/V1 variants may contribute towards the formation of a highly hydrated tumor microenvironment. Furthermore, a broad range of versican fragments are present in canine mammary tumors, among which ADAMTS generated G1-bearing V1/V0 fragments were identified. These fragments are abundant in the myxoid tissues of complex and mixed tumors, indicating regulated versican degradation and a distinct biological role for the truncated versican forms in canine mammary tumors.
In Chapter 5 immunohistochemistry, gelatine/casein zymography, in situ zymography with quenched fluorogenic DQ-gelatin/substrate and quenched fluorescent substrate assay was used to measure the in vivo activity of MMPs and cathepsin B. MMPs were secreted by both tumor cells and stromal fibroblasts; however, in situ zymography showed that gelatinases exert their activity primarily in the tumor stroma, particularly surrounding ductal structures. All methods indicated elevated enzyme expression and activity in tumors and their…
Subjects/Keywords: extracellular matrix; versican; splice variants; catabolic products; cartilage markers; hyaluronan; link protein; MMP; cathepsin B; canine mammary tumors
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APA (6th Edition):
Erdélyi, I. (2006). Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/9192 ; URN:NBN:NL:UI:10-1874-9192 ; 1874/9192 ; urn:isbn:9039341931 ; URN:NBN:NL:UI:10-1874-9192 ; https://dspace.library.uu.nl/handle/1874/9192
Chicago Manual of Style (16th Edition):
Erdélyi, Ildikó. “Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.” 2006. Doctoral Dissertation, University Utrecht. Accessed March 04, 2021.
https://dspace.library.uu.nl/handle/1874/9192 ; URN:NBN:NL:UI:10-1874-9192 ; 1874/9192 ; urn:isbn:9039341931 ; URN:NBN:NL:UI:10-1874-9192 ; https://dspace.library.uu.nl/handle/1874/9192.
MLA Handbook (7th Edition):
Erdélyi, Ildikó. “Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.” 2006. Web. 04 Mar 2021.
Vancouver:
Erdélyi I. Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. [Internet] [Doctoral dissertation]. University Utrecht; 2006. [cited 2021 Mar 04].
Available from: https://dspace.library.uu.nl/handle/1874/9192 ; URN:NBN:NL:UI:10-1874-9192 ; 1874/9192 ; urn:isbn:9039341931 ; URN:NBN:NL:UI:10-1874-9192 ; https://dspace.library.uu.nl/handle/1874/9192.
Council of Science Editors:
Erdélyi I. Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. [Doctoral Dissertation]. University Utrecht; 2006. Available from: https://dspace.library.uu.nl/handle/1874/9192 ; URN:NBN:NL:UI:10-1874-9192 ; 1874/9192 ; urn:isbn:9039341931 ; URN:NBN:NL:UI:10-1874-9192 ; https://dspace.library.uu.nl/handle/1874/9192
Technical University of Lisbon
6.
Pedrosa, Angelina Gonçalves.
Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos.
Degree: 2016, Technical University of Lisbon
URL: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/11516
► Dissertação de Mestrado Integrado em Medicina Veterinária
À semelhança do que é a realidade no universo da Medicina humana, a identificação de parâmetros relevantes a…
(more)
▼ Dissertação de Mestrado Integrado em Medicina Veterinária
À semelhança do que é a realidade no universo da Medicina humana, a identificação de parâmetros relevantes a nível de prognóstico constitui uma área de investimento considerável na investigação do cancro mamário canino. Este trabalho pretende caracterizar tumores mamários caninos quanto às características epidemiológicas, clinicopatológicas e imunofenotípicas, avaliando o seu valor prognóstico pela investigação de associações significativas ou tendenciais entre si e incluindo as que dizem respeito aos estudos de sobrevivência [análise do tempo de sobrevivência (TS) e do tempo livre de doença (TLD)]. Pretende verificar também, em termos temporais concretos, como as diferenças em cada uma das características se traduz em termos de prognóstico. Os dados foram obtidos a partir de uma amostra de 41 cadelas que apresentavam tumores mamários. Os tumores foram analisados por avaliação histopatológica, pela avaliação da expressão de marcadores moleculares (Ki-67, RP, HER-2 e CK5/6) através de técnica imunohistoquímica e pelo estabelecimento de sua respetiva classificação molecular. Em termos epidemiológicos a nossa amostra mostrou-se perfeitamente concordante com a descrita em estudos anteriores. Em termos associativos foi possível concluir, a nível estatisticamente significativo, do pior prognóstico do maior tamanho tumoral, da metastização regional, do maior estadio tumoral TNM, da infiltração de células linfocíticas, dos tipos histopatológicos considerados de maior malignidade, do maior grau histológico de malignidade e da ausência de diferenciação mesenquimatosa. A expressão de Ki-67 como indicador de proliferação revelou-se de valor prognóstico importante, pela associação (estatisticamente significativa) da sua maior expressão às características anteriormente descritas como indicativas de pior prognóstico em si: o tamanho tumoral, o grau de malignidade, a necrose intratumoral, a metastização regional e, em termos epidemiológicos, a cadelas de raça indeterminada. O valor cut-off de 25% para o Ki-67 surgiu como o mais apropriado para o estudo do seu valor prognóstico (quando comparado com os cut-off alternativos de 20% e 16,43%-mediana) pelo número e significância de associações conseguidas. A maior expressão de RP surgiu associada significativamente aos tumores de cadelas inteiras e cadelas de raça indeterminada, assim como a tumores de tipo histológico simples e complexos e graus I e II de malignidade. Verificou-se uma sobre-expressão do marcador HER-2 em 19,4% dos tumores analisados, com uma maior frequência destes em carcinomas simples e de grau histológico III com infiltração linfocítica, não se chegando no entanto a associações estatisticamente significativas As CK5/6 tiveram expressão positiva quase ubiquitária, sendo os carcinomas de células escamosas o tipo histológico com uma maior percentagem de marcação.
Dentro das associações estatisticamente significativas, no que diz respeito aos subtipos moleculares de acordo com o sistema de classificação…
Advisors/Committee Members: Ferreira, Fernando António da Costa, Correia, Jorge Manuel de Jesus.
Subjects/Keywords: Tumores mamários caninos; prognóstico; caracterização clinicopatológica e imunofenotípica; estudo associativo; TS e TLD; estardardização de metodologias e critérios; Canine mammary tumors; prognosis; clinicopathological and immunophenotypical characterization; correlation studies; OS and DFS; standardization of methodologies and criteria; specificity
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pedrosa, A. G. (2016). Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/11516
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pedrosa, Angelina Gonçalves. “Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos.” 2016. Thesis, Technical University of Lisbon. Accessed March 04, 2021.
http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/11516.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pedrosa, Angelina Gonçalves. “Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos.” 2016. Web. 04 Mar 2021.
Vancouver:
Pedrosa AG. Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos. [Internet] [Thesis]. Technical University of Lisbon; 2016. [cited 2021 Mar 04].
Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/11516.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pedrosa AG. Valor prognóstico de características clinicopatológicas e imunofenotípicas em tumores mamários caninos. [Thesis]. Technical University of Lisbon; 2016. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/11516
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universiteit Utrecht
7.
Erdélyi, Ildikó.
Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.
Degree: 2006, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/9192
► The extracellular matrix (ECM) research has become fundamental to understand cancer. This thesis focuses on the exploration of ECM composition and organization in canine mammary…
(more)
▼ The extracellular matrix (ECM) research has become fundamental to understand cancer. This thesis focuses on the exploration of ECM composition and organization in canine mammary tumors, with a special interest in the large chondroitin-sulfate proteoglycan (PG), versican.
Chapter 1 gives an overview of the recent concepts in matrix biology, particularly in tumors, and introduces versican and other ECM molecules investigated in the following chapters.
In Chapter 2 we show that versican protein expression is increased in tumors. Versican accumulates at three main locations in tumors: (1) in periductal tissues; (2) in peripheral invasive areas; (3) and the G1-domain of versican in myoepithelial-like spindle cell proliferations and myxoid tissues of complex and mixed tumors.
In Chapter 3 we show up-regulated mRNA expression of versican, collagen type II and aggrecan in tumors, especially in complex and mixed tumors. Expression of cartilage biomarkers was noted not only in the cartilagenous tissues of mixed tumors, but in myoepitheliomas and in myoepithelial-like cell proliferations and myxoid tissues of complex and mixed tumors. Therefore, the latter tissues appear to be the precursor tissues of the ectopic cartilage in mixed tumors. In addition, co-localization of hyaluronan (HA) and link protein (LP) with versican surrounding invading tumor cells was demonstrated. The three molecules may form complexes in canine mammary tumors and provide a viscoelastic microenvironment that promotes tumor cell proliferation and migration.
Chapter 4 shows that versican is present in multiple forms, including splice variants and catabolic products. V1, V0 and V3 versican mRNA and protein have been identified, with a prevalence of V1/V0 variants in canine mammary tumors and cell lines. The tumor cell lines expressed increased mRNA and protein of V0 and V1 versican compared to the normal mammary fibroblasts and the myoepithelial-like tumor cells expressed the highest levels of all mRNA splice variants. The high expression of the hydrophilic V0/V1 variants may contribute towards the formation of a highly hydrated tumor microenvironment. Furthermore, a broad range of versican fragments are present in canine mammary tumors, among which ADAMTS generated G1-bearing V1/V0 fragments were identified. These fragments are abundant in the myxoid tissues of complex and mixed tumors, indicating regulated versican degradation and a distinct biological role for the truncated versican forms in canine mammary tumors.
In Chapter 5 immunohistochemistry, gelatine/casein zymography, in situ zymography with quenched fluorogenic DQ-gelatin/substrate and quenched fluorescent substrate assay was used to measure the in vivo activity of MMPs and cathepsin B. MMPs were secreted by both tumor cells and stromal fibroblasts; however, in situ zymography showed that gelatinases exert their activity primarily in the tumor stroma, particularly surrounding ductal structures. All methods indicated elevated enzyme expression and activity in tumors and their…
Subjects/Keywords: Diergeneeskunde; extracellular matrix; versican; splice variants; catabolic products; cartilage markers; hyaluronan; link protein; MMP; cathepsin B; canine mammary tumors
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APA (6th Edition):
Erdélyi, I. (2006). Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/9192
Chicago Manual of Style (16th Edition):
Erdélyi, Ildikó. “Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.” 2006. Doctoral Dissertation, Universiteit Utrecht. Accessed March 04, 2021.
http://dspace.library.uu.nl:8080/handle/1874/9192.
MLA Handbook (7th Edition):
Erdélyi, Ildikó. “Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan.” 2006. Web. 04 Mar 2021.
Vancouver:
Erdélyi I. Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2006. [cited 2021 Mar 04].
Available from: http://dspace.library.uu.nl:8080/handle/1874/9192.
Council of Science Editors:
Erdélyi I. Extracellular matrix in canine mammary tumors with special focus on versican, a versatile extracellular proteoglycan. [Doctoral Dissertation]. Universiteit Utrecht; 2006. Available from: http://dspace.library.uu.nl:8080/handle/1874/9192
8.
L. Terzaghi.
ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.
Degree: 2018, Università degli Studi di Milano
URL: http://hdl.handle.net/2434/550339
► La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi…
(more)
▼ La sub-fertilità rappresenta uno dei principali problemi che l'industria lattiero-casearia si trova ad affrontare attualmente. Si tratta di un problema multifattoriale che dipende da diversi aspetti della funzione riproduttiva. La qualità dell'ovocita, determinata durante la follicologenesi, è sicuramente cruciale per ottenere risultati riproduttivi ottimali. Per migliorare la fertilità femminile nel bovino è fondamentale definire i fattori e i meccanismi che determinano la qualità del gamete femminile, in quanto il declino della funzione riproduttiva in questa specie è dovuto principalmente alla scarsa qualità degli ovociti. In particolare, è importante conoscere quali proteine regolano la meiosi, la fecondabilità e lo sviluppo embrionale dell'ovocita. In questo progetto, abbiamo considerato principalmente il ruolo del Progesterone Receptor Membrane Component-1 (PGRMC1) sia nell'ovocita che nel compartimento follicolare, considerato che il dialogo tra compartimento germinale e compartimento somatico è uno dei fattori chiave coinvolti nell'acquisizione della competenza allo sviluppo del gamete femminile.
Il PGRMC1 è una proteina multifunzionale ed altamente conservata in specie anche filogeneticamente distanti. Nei mammiferi è espressa in diversi sistemi, compresi gli organi riproduttivi, e localizza in diversi compartimenti subcellulari. Tuttavia, il suo ruolo e il preciso meccanismo d'azione in ogni sistema e/o compartimento subcellulare non sono ancora del tutto conosciuti.
La parte più considerevole di questo progetto di dottorato riguarda gli studi effettuati sul ruolo del PGRMC1 nella divisione cellulare, data la sua localizzazione a livello di fuso mitotico e meiotico. Abbiamo dimostrato che la deplezione del PGRMC1 determina difetti nella divisione cellulare e la sua interazione con l'Aurora chinasi B (AURKB) indica che potrebbe svolgere la sua azione durante la citodieresi, l'ultima fase della divisione. Durante la maturazione meiotica, abbiamo valutato anche il suo ruolo nel mediare l'azione del progesterone (P4) confrontando il suo effetto con quello del recettore nucleare del progesterone (nPGR). L'inibizione di entrambi i tipi di recettore determina lo stesso effetto sull'organizzazione della piastra metafasica e sulla competenza allo sviluppo dell'ovocita, ma in fasi differenti.
Inoltre, abbiamo testato l'ipotesi che il PGRMC1 possa modulare la funzione del nucleolo. Studi di immunofluorescenza hanno confermato la presenza del PGRMC1 nel nucleolo di cellule della granulosa (bGC) e negli ovociti di bovino, nonchè la colocalizzazione con la nucleolina, la proteina nucleolare più abbondante che svolge importanti funzioni in questo compartimento
subcellulare. Inoltre, la down-regolazione del PGRMC1 determina uno spostamento della nucleolina dal nucleolo al nucleoplasma suggerendo un'associazione funzionale tra queste due proteine. Questa interazione è probabilmente mediata dalla presenza di ulteriori molecole in quanto successivi esperimenti di "in-situ proximity ligation" mostrano che le due…
Advisors/Committee Members: tutor: V. Lodde, cotutor: A. M. Luciano, coordinatore: F. Gandolfi, LODDE, VALENTINA, GANDOLFI, FULVIO.
Subjects/Keywords: Progesterone Receptor Membrane Component 1; PGRMC1; fertility; reproduction; cell division; meiosis; mitosis; bovine granulosa cells; bovine oocyte; nucleolin; Canine Mammary tumors; progesterone; Settore VET/01 - Anatomia degli Animali Domestici; Settore VET/02 - Fisiologia Veterinaria; Settore BIO/06 - Anatomia Comparata e Citologia; Settore BIO/09 - Fisiologia; Settore BIO/11 - Biologia Molecolare; Settore BIO/17 - Istologia
Record Details
Similar Records
Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Terzaghi, L. (2018). ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. (Thesis). Università degli Studi di Milano. Retrieved from http://hdl.handle.net/2434/550339
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Thesis, Università degli Studi di Milano. Accessed March 04, 2021.
http://hdl.handle.net/2434/550339.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Terzaghi, L.. “ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION.” 2018. Web. 04 Mar 2021.
Vancouver:
Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Internet] [Thesis]. Università degli Studi di Milano; 2018. [cited 2021 Mar 04].
Available from: http://hdl.handle.net/2434/550339.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Terzaghi L. ROLE OF PROGESTERONE RECEPTOR MEMBRANE COMPONENT 1 (PGRMC1) IN CONTROLLING GERMINAL AND SOMATIC CELL DIVISION AND FUNCTION. [Thesis]. Università degli Studi di Milano; 2018. Available from: http://hdl.handle.net/2434/550339
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
. 
Open Access Theses and Dissertations